FastQCFastQC Report
Mon 6 Jun 2016
ERR1211462_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211462_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3798693
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145220.38228938216381264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131360.3458031486092717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77580.20422813846762555No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39140.10303543876801838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69600.058.697651
TACCTGG30300.041.56312
GTATCAA120650.038.2253841
GTGGTAT24200.033.2193341
ACCTGGG38150.032.7648773
ATAACGC3300.031.3278223
ATCAACG146300.030.9959143
CAACGCA147350.030.7411255
TATCAAC151700.030.078032
TCAACGC154750.029.4564024
AACGCAG159650.028.7538286
TGGTATC27750.028.6180132
GTACATG119200.028.2782841
TAACGCA4150.027.1770154
TACATGG121650.026.9238012
ACATGGG121800.025.9650523
ACGCAGA176200.025.7597267
CCTGGGG35500.025.6810044
CGCAGAG179250.025.2689918
TGGGGGG73100.024.6821986
Produced by FastQC (version 0.11.5)