##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211462_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3798693 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05491230799646 33.0 31.0 34.0 30.0 34.0 2 32.189198758625665 33.0 31.0 34.0 30.0 34.0 3 32.263707543620924 34.0 31.0 34.0 30.0 34.0 4 35.91047289159719 37.0 35.0 37.0 35.0 37.0 5 35.767748012276854 37.0 35.0 37.0 35.0 37.0 6 35.80700046042152 37.0 35.0 37.0 35.0 37.0 7 35.73838949343893 37.0 35.0 37.0 33.0 37.0 8 35.72809016153714 37.0 35.0 37.0 33.0 37.0 9 37.31134076904872 39.0 37.0 39.0 34.0 39.0 10-11 37.28999934977635 39.0 37.0 39.0 34.0 39.0 12-13 37.20531588101487 39.0 37.0 39.0 33.0 39.0 14-15 38.40694852150463 40.0 38.0 41.0 33.5 41.0 16-17 38.391058319269284 40.0 38.0 41.0 33.5 41.0 18-19 38.400143680997644 40.0 38.0 41.0 33.5 41.0 20-21 38.36321137296433 40.0 38.0 41.0 34.0 41.0 22-23 38.260049048449034 40.0 38.0 41.0 34.0 41.0 24-25 38.141121564706594 40.0 37.0 41.0 33.0 41.0 26-27 38.008702466874794 40.0 37.0 41.0 33.0 41.0 28-29 37.92669347062266 40.0 37.0 41.0 33.0 41.0 30-31 37.95857272488195 40.0 37.0 41.0 33.0 41.0 32-33 37.78561915901074 40.0 37.0 41.0 33.0 41.0 34-35 37.669640584274646 40.0 37.0 41.0 33.0 41.0 36-37 37.51646408383094 40.0 36.0 41.0 32.0 41.0 38-39 37.39454149098124 40.0 36.0 41.0 32.0 41.0 40-41 37.31598828860348 40.0 36.0 41.0 32.0 41.0 42-43 37.17084744674024 39.5 36.0 41.0 31.0 41.0 44-45 37.0091659157505 39.0 36.0 41.0 31.0 41.0 46-47 36.772293917934405 39.0 35.0 41.0 30.5 41.0 48-49 36.539716950014125 39.0 35.0 41.0 30.0 41.0 50-51 36.35096229150395 39.0 35.0 41.0 29.5 41.0 52-53 36.14097954217411 39.0 35.0 40.0 29.0 41.0 54-55 35.935293007358055 38.5 35.0 40.0 28.5 41.0 56-57 35.70477266786234 38.0 34.5 40.0 28.0 41.0 58-59 35.43753311994415 38.0 34.0 40.0 27.5 41.0 60-61 35.17949463144297 37.5 34.0 40.0 27.0 41.0 62-63 34.88508863443295 37.0 34.0 40.0 26.5 41.0 64-65 34.538867710552026 36.5 33.5 39.5 26.0 41.0 66-67 34.18321117289552 36.0 33.0 39.0 26.0 40.5 68-69 33.810997493085125 35.5 33.0 39.0 25.5 40.0 70-71 33.435285768026006 35.0 32.5 38.0 25.0 40.0 72-73 33.075704854274875 35.0 32.0 37.5 24.5 39.5 74-75 32.70411941686259 35.0 32.0 37.0 24.0 39.0 76-77 31.666624283668092 34.0 30.5 36.0 22.5 38.0 78-79 32.13614090425312 34.5 31.0 36.0 24.0 38.0 80-81 32.02287681578901 35.0 31.0 35.5 24.0 37.0 82-83 31.748822292298954 34.0 31.0 35.0 23.0 37.0 84-85 31.479704993270055 34.0 31.0 35.0 23.0 36.5 86-87 31.22502910869607 34.0 31.0 35.0 21.5 36.0 88-89 30.990539377622778 34.0 31.0 35.0 20.0 36.0 90-91 30.768627130436705 34.0 30.0 35.0 20.0 35.0 92-93 30.58556337666666 34.0 30.0 35.0 20.0 35.0 94-95 30.41838008493974 34.0 30.0 35.0 19.0 35.0 96-97 30.244516732465613 34.0 29.5 35.0 19.0 35.0 98-99 30.0367955504696 34.0 29.0 35.0 18.0 35.0 100 28.222232752159755 32.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.23578385725871343 1101 2 -0.21315375014754423 1101 3 -0.1992421075555697 1101 4 -0.13482976250628553 1101 5 -0.1266166745275541 1101 6 -0.14106598842655416 1101 7 -0.18338016005665025 1101 8 -0.21444847329550498 1101 9 -0.4614106409367196 1101 10-11 -0.38405389998401773 1101 12-13 -0.4368833536410861 1101 14-15 -0.5654730634313054 1101 16-17 -0.5678601573251143 1101 18-19 -0.5426004729033167 1101 20-21 -0.459487064870963 1101 22-23 -0.5707671466688424 1101 24-25 -0.5691647949803311 1101 26-27 -0.6145288394259225 1101 28-29 -0.7268864441582679 1101 30-31 -0.48069200935594836 1101 32-33 -0.49168215634517765 1101 34-35 -0.5431391606810436 1101 36-37 -0.5125910656905504 1101 38-39 -0.5037293239664535 1101 40-41 -0.5813777302900149 1101 42-43 -0.6243625021713939 1101 44-45 -0.599650561871492 1101 46-47 -0.6069347683374886 1101 48-49 -0.5217908776460973 1101 50-51 -0.547450591519727 1101 52-53 -0.6144071525309798 1101 54-55 -0.7095542246385449 1101 56-57 -0.6322109284643389 1101 58-59 -0.6065070772648085 1101 60-61 -0.5862928483227847 1101 62-63 -0.6283166457368736 1101 64-65 -0.6967702596725474 1101 66-67 -0.669535071681139 1101 68-69 -0.7166252318811743 1101 70-71 -0.8124659212958534 1101 72-73 -0.7408274427465642 1101 74-75 -0.8105363030888562 1101 76-77 -0.6651092104529042 1101 78-79 -0.6285699542677072 1101 80-81 -0.527555024401714 1101 82-83 -0.5293609625901929 1101 84-85 -0.7053469006896513 1101 86-87 -0.8392393183203701 1101 88-89 -0.8550400201759558 1101 90-91 -1.0273787871843147 1101 92-93 -1.024703292948562 1101 94-95 -1.1312318376412271 1101 96-97 -1.1980821291775499 1101 98-99 -1.1410577049773671 1101 100 -1.1485363262904187 1102 1 -0.30312399720888905 1102 2 -0.2725395252608216 1102 3 -0.336185934995612 1102 4 -0.1537664063378017 1102 5 -0.1857404723673568 1102 6 -0.2847717048824805 1102 7 -0.2460095013515371 1102 8 -0.25241600888008264 1102 9 -0.3452069472169086 1102 10-11 -0.4987663998941656 1102 12-13 -0.4844553914009566 1102 14-15 -0.5971144069593919 1102 16-17 -0.6856385101749609 1102 18-19 -0.7030719361502236 1102 20-21 -0.6658456846021608 1102 22-23 -0.696860083106607 1102 24-25 -0.7990358750055933 1102 26-27 -0.7592221622801603 1102 28-29 -0.6723819925413892 1102 30-31 -0.670214595710604 1102 32-33 -0.6807970006324382 1102 34-35 -0.7307480350014472 1102 36-37 -0.5723630176688417 1102 38-39 -0.6005638717401638 1102 40-41 -0.8143170593885785 1102 42-43 -0.7115805671018265 1102 44-45 -0.7128203510996016 1102 46-47 -0.7715547375551566 1102 48-49 -0.7544318881116041 1102 50-51 -0.9660591247904478 1102 52-53 -0.8387902879037483 1102 54-55 -0.7255072827839228 1102 56-57 -0.6458397370188536 1102 58-59 -0.7722576221855206 1102 60-61 -0.7983726363767829 1102 62-63 -0.7674269773058597 1102 64-65 -0.756435583705688 1102 66-67 -0.660396617490818 1102 68-69 -0.8164337808066335 1102 70-71 -0.7676614178426249 1102 72-73 -0.8505004743427804 1102 74-75 -0.8964145299819393 1102 76-77 -0.7634804785746603 1102 78-79 -0.6697846502342983 1102 80-81 -0.6964317249576979 1102 82-83 -0.6827438639405727 1102 84-85 -0.7863054659487503 1102 86-87 -0.7959140280088164 1102 88-89 -0.8148752300812525 1102 90-91 -0.8530153400426279 1102 92-93 -0.9123621665900572 1102 94-95 -1.0081368636911954 1102 96-97 -1.089244150140523 1102 98-99 -1.1745440713765447 1102 100 -1.306117253705942 1103 1 -0.24223111952616705 1103 2 -0.2103494158734378 1103 3 -0.21544313518552372 1103 4 -0.16247637777293988 1103 5 -0.1256258256197995 1103 6 -0.29928355007073293 1103 7 -0.2561591099437095 1103 8 -0.4069257445580874 1103 9 -0.5016372199319079 1103 10-11 -0.49940276581511256 1103 12-13 -0.6259572762240353 1103 14-15 -0.7073243824928568 1103 16-17 -0.6988639774580463 1103 18-19 -0.6896419130275717 1103 20-21 -0.5868878060813074 1103 22-23 -0.6992808406874005 1103 24-25 -0.7127861797901929 1103 26-27 -0.8944392918636268 1103 28-29 -0.8544936158977947 1103 30-31 -0.6840989021343518 1103 32-33 -0.6882476160698872 1103 34-35 -0.7306348132091429 1103 36-37 -0.6375874620826565 1103 38-39 -0.6960647871973435 1103 40-41 -0.7033555595718326 1103 42-43 -0.772040733972915 1103 44-45 -0.6087028248975841 1103 46-47 -0.7663771599376545 1103 48-49 -0.7886878754682343 1103 50-51 -0.7389616550588087 1103 52-53 -0.8098728836966274 1103 54-55 -0.7771831229378066 1103 56-57 -0.883997678806999 1103 58-59 -0.9486726221184085 1103 60-61 -0.8392295352904426 1103 62-63 -0.9307989078310897 1103 64-65 -0.8327236076953781 1103 66-67 -0.9176553548669801 1103 68-69 -0.8669443462373394 1103 70-71 -0.857918326826443 1103 72-73 -0.9595523359585485 1103 74-75 -0.9286191942694195 1103 76-77 -0.9098558995196875 1103 78-79 -0.7890906952241501 1103 80-81 -0.6262063361282095 1103 82-83 -0.5420792078839689 1103 84-85 -0.7552196437401122 1103 86-87 -0.8195540536868329 1103 88-89 -0.7969611015598375 1103 90-91 -1.0287869933951477 1103 92-93 -0.9680831412558462 1103 94-95 -0.938083964704397 1103 96-97 -0.9874490217761043 1103 98-99 -1.1800338871348721 1103 100 -1.0003756297991941 1104 1 -0.3313807665767001 1104 2 -0.3065929380316774 1104 3 -0.3033637094713555 1104 4 -0.1689416146608167 1104 5 -0.16570498335566697 1104 6 -0.339373208251736 1104 7 -0.34954087028867065 1104 8 -0.2534482878422679 1104 9 -0.4706025034643204 1104 10-11 -0.4074887247153285 1104 12-13 -0.48656712125048074 1104 14-15 -0.541775449160184 1104 16-17 -0.6405374325323265 1104 18-19 -0.5925150951597047 1104 20-21 -0.6294572615360394 1104 22-23 -0.6525220113533905 1104 24-25 -0.7061893402159072 1104 26-27 -0.7608822209463071 1104 28-29 -0.6930546956420827 1104 30-31 -0.7535587392767837 1104 32-33 -0.5452685217619262 1104 34-35 -0.7349982254580922 1104 36-37 -0.6300114343038601 1104 38-39 -0.8127471220902152 1104 40-41 -0.7236192057897952 1104 42-43 -0.7507438786653182 1104 44-45 -0.8272390003629724 1104 46-47 -0.7178204114100382 1104 48-49 -0.8749746325584908 1104 50-51 -0.7933458035174041 1104 52-53 -0.8380052684492512 1104 54-55 -0.7566647324495079 1104 56-57 -0.7622309589525429 1104 58-59 -0.8958983828170872 1104 60-61 -0.8926134946542348 1104 62-63 -0.8590639453146451 1104 64-65 -0.7627373598072964 1104 66-67 -0.9189219602145116 1104 68-69 -0.7572117873682487 1104 70-71 -0.8236631411902877 1104 72-73 -0.7714937892947802 1104 74-75 -0.7083746571260008 1104 76-77 -0.6708698034107599 1104 78-79 -0.6162315429016587 1104 80-81 -0.689941261155468 1104 82-83 -0.5915778289846045 1104 84-85 -0.5524537917778183 1104 86-87 -0.49920812216240407 1104 88-89 -0.6930993328908883 1104 90-91 -0.5714158501283784 1104 92-93 -0.7814667569687153 1104 94-95 -0.7765317524558633 1104 96-97 -0.7373447332641376 1104 98-99 -0.7587597162600055 1104 100 -0.8651029944191677 1105 1 -0.36945978644989097 1105 2 -0.33400414087036623 1105 3 -0.3865616314308049 1105 4 -0.2178799559511404 1105 5 -0.22448332960967576 1105 6 -0.3007901059142739 1105 7 -0.29940269199506986 1105 8 -0.2602736080084185 1105 9 -0.3955031878157911 1105 10-11 -0.3587073432342365 1105 12-13 -0.41707719032286406 1105 14-15 -0.59094705016701 1105 16-17 -0.53914705537602 1105 18-19 -0.6598490925537703 1105 20-21 -0.7217914266222039 1105 22-23 -0.6118675761487822 1105 24-25 -0.6312054805809098 1105 26-27 -0.6370570299030831 1105 28-29 -0.6326431959883649 1105 30-31 -0.6252424183073941 1105 32-33 -0.5152114729081774 1105 34-35 -0.5331046881440855 1105 36-37 -0.6803609046483459 1105 38-39 -0.707908576047835 1105 40-41 -0.5944371743341108 1105 42-43 -0.5748155771012478 1105 44-45 -0.6343584524793258 1105 46-47 -0.6934950786669845 1105 48-49 -0.6873466205986958 1105 50-51 -0.6679052763605071 1105 52-53 -0.7150481355817675 1105 54-55 -0.7868456219219695 1105 56-57 -0.8187314595134723 1105 58-59 -0.8589430258089834 1105 60-61 -0.7404083854112145 1105 62-63 -0.674641619458697 1105 64-65 -0.8108600467043345 1105 66-67 -0.8576406835409927 1105 68-69 -0.75033548880252 1105 70-71 -0.6626799372314167 1105 72-73 -0.7186151866395463 1105 74-75 -0.7649939675425337 1105 76-77 -0.7246114754016517 1105 78-79 -0.7128584162320735 1105 80-81 -0.5756011547380595 1105 82-83 -0.6034988510344412 1105 84-85 -0.4551327569833852 1105 86-87 -0.5468990018919513 1105 88-89 -0.5663757096011395 1105 90-91 -0.6340064667490104 1105 92-93 -0.6924364617132248 1105 94-95 -0.6858500672843739 1105 96-97 -0.7525145802115567 1105 98-99 -0.6273355994523904 1105 100 -0.6031732104569052 1106 1 -0.34906223404993497 1106 2 -0.3551834179451987 1106 3 -0.4130546110153226 1106 4 -0.1729939526243669 1106 5 -0.2575632816669966 1106 6 -0.3174933493495473 1106 7 -0.2427557911706799 1106 8 -0.2810528093842777 1106 9 -0.42159160952546415 1106 10-11 -0.4713462491849043 1106 12-13 -0.5888242096758987 1106 14-15 -0.6550101260021037 1106 16-17 -0.679852267648549 1106 18-19 -0.7714385053223296 1106 20-21 -0.6470679934746926 1106 22-23 -0.6496224376495476 1106 24-25 -0.7197636476033793 1106 26-27 -0.705327706844443 1106 28-29 -0.7872019008719491 1106 30-31 -0.7705338095415826 1106 32-33 -0.7994016765317724 1106 34-35 -0.6482596718256417 1106 36-37 -0.7468943234197027 1106 38-39 -0.7803950714179138 1106 40-41 -0.7720342632438744 1106 42-43 -0.7862248728020163 1106 44-45 -0.8071503168320859 1106 46-47 -0.8406543751977793 1106 48-49 -0.8306532379503508 1106 50-51 -0.8789542549224265 1106 52-53 -0.828072674664682 1106 54-55 -0.9144742520694109 1106 56-57 -0.9243771750513474 1106 58-59 -0.8174003106728378 1106 60-61 -0.9639465320480838 1106 62-63 -0.9162250891940502 1106 64-65 -0.898979956529594 1106 66-67 -0.821074511684408 1106 68-69 -0.8698963683714354 1106 70-71 -0.8032711030287629 1106 72-73 -0.8527225530126046 1106 74-75 -0.8507353360338357 1106 76-77 -0.8780256483737965 1106 78-79 -0.7727069186873088 1106 80-81 -0.7728713436202703 1106 82-83 -0.76403918563717 1106 84-85 -0.7878053290551108 1106 86-87 -0.6738395350384714 1106 88-89 -0.6526996779536596 1106 90-91 -0.7102335869012073 1106 92-93 -0.7955707477026088 1106 94-95 -0.8615834010603898 1106 96-97 -0.9389324252477955 1106 98-99 -1.00610959154384 1106 100 -0.9599711772091197 1107 1 -0.3374025568253707 1107 2 -0.38326169029991064 1107 3 -0.3911668882258539 1107 4 -0.2338394193750517 1107 5 -0.298676321624221 1107 6 -0.3329131018941496 1107 7 -0.4168441551030426 1107 8 -0.3828525840520598 1107 9 -0.5098091432171756 1107 10-11 -0.5382983345330885 1107 12-13 -0.522143778757659 1107 14-15 -0.702916849224998 1107 16-17 -0.7434121163549676 1107 18-19 -0.7759552407408421 1107 20-21 -0.7174746229960363 1107 22-23 -0.7727364335228302 1107 24-25 -0.7190762625880112 1107 26-27 -0.8131906129766264 1107 28-29 -0.6732918005488102 1107 30-31 -0.7448614247840908 1107 32-33 -0.7167444092792294 1107 34-35 -0.713264606774338 1107 36-37 -0.6529226331429143 1107 38-39 -0.8074262088458966 1107 40-41 -0.8399947215069616 1107 42-43 -0.6235407091981386 1107 44-45 -0.6628825136162462 1107 46-47 -0.6351050325982968 1107 48-49 -0.651781529638022 1107 50-51 -0.6283694610994814 1107 52-53 -0.7092995435943052 1107 54-55 -0.67590616243114 1107 56-57 -0.712601003565652 1107 58-59 -0.6702278392614289 1107 60-61 -0.7371043514190347 1107 62-63 -0.6487251620730419 1107 64-65 -0.46001362726327955 1107 66-67 -0.4645241481383806 1107 68-69 -0.5049897479799839 1107 70-71 -0.5369871338115644 1107 72-73 -0.6079853770718273 1107 74-75 -0.5514930052260141 1107 76-77 -0.6273355404071772 1107 78-79 -0.5785793154257917 1107 80-81 -0.5395866603491548 1107 82-83 -0.5762360370726292 1107 84-85 -0.6418703588970551 1107 86-87 -0.6522059276694634 1107 88-89 -0.5528646727816522 1107 90-91 -0.5617593045350056 1107 92-93 -0.5118771091894416 1107 94-95 -0.6445130114893551 1107 96-97 -0.6256129170320079 1107 98-99 -0.7024163566681523 1107 100 -0.8450568684847504 1108 1 -0.4006291203252488 1108 2 -0.40711273332595255 1108 3 -0.3600593324108239 1108 4 -0.3016194305858022 1108 5 -0.3162215150439778 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1.310244742791859 2210 72-73 1.3128743225169828 2210 74-75 1.3545382144342497 2210 76-77 1.4061778972937482 2210 78-79 1.2178957050580053 2210 80-81 1.1517134771514286 2210 82-83 1.162184237253765 2210 84-85 1.1046218780872543 2210 86-87 1.1868532877136424 2210 88-89 1.2494973007615755 2210 90-91 1.2807114210196033 2210 92-93 1.2699004887025325 2210 94-95 1.340192926175341 2210 96-97 1.3388048637945715 2210 98-99 1.348720364757174 2210 100 1.68259172294729 2211 1 0.5883866333057526 2211 2 0.5704048613600108 2211 3 0.5048628074822616 2211 4 0.3723516719125115 2211 5 0.40970454977409787 2211 6 0.44147835678256087 2211 7 0.3811790686043892 2211 8 0.4497654106263411 2211 9 0.5350677055246464 2211 10-11 0.6385830868504812 2211 12-13 0.6517119124037904 2211 14-15 0.8549594862305483 2211 16-17 0.7793497957063096 2211 18-19 0.7712491215933284 2211 20-21 0.8334548720411163 2211 22-23 0.8800738338004592 2211 24-25 0.8277611724585583 2211 26-27 0.8380055862084035 2211 28-29 0.8385682705223658 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2311 40-41 0.9516827774579539 2311 42-43 1.0699131508898034 2311 44-45 1.0123212385190428 2311 46-47 1.0541670665863023 2311 48-49 1.0648237601774966 2311 50-51 1.005057912630484 2311 52-53 1.0573931205881593 2311 54-55 1.0004671693023397 2311 56-57 1.0799448692114453 2311 58-59 1.0783837748880387 2311 60-61 1.0080553703556987 2311 62-63 1.09793602116698 2311 64-65 1.057727516977998 2311 66-67 1.1125006522078564 2311 68-69 1.1741180138215341 2311 70-71 1.197287578703559 2311 72-73 1.121985672608119 2311 74-75 1.0891205954002956 2311 76-77 1.0348651332568473 2311 78-79 0.9752854757657659 2311 80-81 0.9933181583755761 2311 82-83 0.9006710461270764 2311 84-85 1.0163337213550285 2311 86-87 1.0776096430402013 2311 88-89 1.150205428914802 2311 90-91 1.220169187730054 2311 92-93 1.1529580040972647 2311 94-95 1.1958265659386775 2311 96-97 1.2194044232863348 2311 98-99 1.3173732338080164 2311 100 1.4486798781520527 2312 1 0.1737958100002288 2312 2 0.11230143554757177 2312 3 0.2891959521343068 2312 4 0.2571486752519121 2312 5 0.3885971688373502 2312 6 0.40584692257264265 2312 7 0.48135900750676797 2312 8 0.2399217540587486 2312 9 0.3799637036922334 2312 10-11 0.2787574072137531 2312 12-13 0.4323793643224789 2312 14-15 0.7705269447135166 2312 16-17 0.8283407481823701 2312 18-19 0.8033952060042964 2312 20-21 0.8972650705674923 2312 22-23 0.48969944276648647 2312 24-25 0.5115580070023569 2312 26-27 0.769497293376368 2312 28-29 0.9042047436600171 2312 30-31 0.8393669670589432 2312 32-33 0.7071921697280317 2312 34-35 0.8863815669460919 2312 36-37 0.9630945627198102 2312 38-39 0.9559868971947196 2312 40-41 0.9173628104646241 2312 42-43 0.8242402695256743 2312 44-45 0.8083383685231595 2312 46-47 0.9354561883845278 2312 48-49 0.9531808634238743 2312 50-51 0.9594819025288004 2312 52-53 0.9259872382856997 2312 54-55 1.0341598260581293 2312 56-57 0.9935438225795608 2312 58-59 0.9747819871056578 2312 60-61 0.9452234205381558 2312 62-63 0.9728058556492201 2312 64-65 1.0684728623676065 2312 66-67 1.0144761639735407 2312 68-69 1.0235681196952484 2312 70-71 0.8338253490741678 2312 72-73 0.8967248902170368 2312 74-75 1.0123240004858474 2312 76-77 1.0058195643007117 2312 78-79 0.9300536057814313 2312 80-81 0.791859821157626 2312 82-83 0.7357591660244758 2312 84-85 0.6078899637602966 2312 86-87 0.8219785261241128 2312 88-89 0.5306802362205048 2312 90-91 0.9232169377941055 2312 92-93 1.0089468215289195 2312 94-95 1.155425113037456 2312 96-97 1.2029241245372972 2312 98-99 1.1877960074634153 2312 100 1.2653102439927508 2313 1 -0.25603916327054677 2313 2 0.027994018772950824 2313 3 0.23030864375844118 2313 4 0.1290143150490053 2313 5 0.20249136802785728 2313 6 0.3919336219772944 2313 7 0.08977962024533781 2313 8 0.08898821577489002 2313 9 0.3141014913673317 2313 10-11 0.32951691592052157 2313 12-13 0.5815120390150668 2313 14-15 0.6021889786279644 2313 16-17 0.7739488363581373 2313 18-19 0.6557230194334593 2313 20-21 0.8842037132093168 2313 22-23 0.9565528945148145 2313 24-25 0.7657004832931946 2313 26-27 0.9231819876921534 2313 28-29 0.8490803336327346 2313 30-31 0.8124701505153382 2313 32-33 0.942065307586077 2313 34-35 1.0305598331344044 2313 36-37 0.8115809258456466 2313 38-39 0.6417256403516802 2313 40-41 0.7744996892303888 2313 42-43 0.8335421587516407 2313 44-45 0.9433234098396284 2313 46-47 0.9423325226161765 2313 48-49 0.8835228716763837 2313 50-51 0.8195384834576984 2313 52-53 0.8046910879097169 2313 54-55 0.8711024820531037 2313 56-57 0.9258923603959346 2313 58-59 0.7628096597391334 2313 60-61 0.7803750625631238 2313 62-63 0.8542753662200724 2313 64-65 0.9984179945867666 2313 66-67 0.9485865344192632 2313 68-69 1.0166615015534077 2313 70-71 0.9869480514417504 2313 72-73 1.0192666746626315 2313 74-75 0.6654752471538856 2313 76-77 0.8109015621528108 2313 78-79 0.5772988529226311 2313 80-81 0.7140412466732222 2313 82-83 0.7548947260190566 2313 84-85 0.878049712856626 2313 86-87 0.9823391565312356 2313 88-89 0.8741165858116915 2313 90-91 1.0157492538624773 2313 92-93 0.7506496181745241 2313 94-95 0.8124211667205365 2313 96-97 1.0423568235942184 2313 98-99 1.1635969580841738 2313 100 1.2315081078892156 2314 1 -0.41154348227708226 2314 2 -0.18448656454694756 2314 3 0.10419181075262429 2314 4 -0.21244055098610914 2314 5 0.1455631528165 2314 6 0.20993630057229495 2314 7 0.232009330277549 2314 8 0.1783262149988829 2314 9 0.3026622916184749 2314 10-11 0.47233883588287284 2314 12-13 0.6058407427319565 2314 14-15 0.6932818146208106 2314 16-17 0.8344368158212276 2314 18-19 0.8439972533209144 2314 20-21 0.5179218714207678 2314 22-23 0.736039778534348 2314 24-25 0.8131515354070942 2314 26-27 0.8538750276182938 2314 28-29 0.76613614105041 2314 30-31 0.7474258152480786 2314 32-33 0.8899740511362353 2314 34-35 0.8800648229291923 2314 36-37 0.8288917068676582 2314 38-39 0.8286873788327611 2314 40-41 0.8564395511262504 2314 42-43 0.6478644518067256 2314 44-45 0.7544457608954787 2314 46-47 0.8997838795475062 2314 48-49 0.7840609456518735 2314 50-51 0.8851782097715315 2314 52-53 0.9338766774712539 2314 54-55 1.0454674030180229 2314 56-57 0.9405129139785657 2314 58-59 0.9474033419089452 2314 60-61 1.0108886352949327 2314 62-63 0.6684243359086821 2314 64-65 0.852450852575835 2314 66-67 0.9402599621453476 2314 68-69 1.043723710137023 2314 70-71 1.0822258122852233 2314 72-73 1.0616224803520637 2314 74-75 1.163460104849726 2314 76-77 1.029306547522527 2314 78-79 1.0074248469740397 2314 80-81 0.9828948691671862 2314 82-83 0.9697457633573912 2314 84-85 0.8056055072649677 2314 86-87 1.0358671143665354 2314 88-89 1.1108317687771674 2314 90-91 1.2206672076468372 2314 92-93 1.2333010910694178 2314 94-95 1.2620164717345297 2314 96-97 1.3863301367811154 2314 98-99 1.402557724378088 2314 100 1.411943501123634 2315 1 -0.12647210304458767 2315 2 0.11638088459319818 2315 3 0.13525771938796538 2315 4 -0.03690315553508583 2315 5 0.24823888233802904 2315 6 0.3883157338868273 2315 7 0.40461742316502836 2315 8 0.36096367417354003 2315 9 0.5832253965391629 2315 10-11 0.5933806317329058 2315 12-13 0.63342668165307 2315 14-15 0.6758072670954647 2315 16-17 0.7399064485170612 2315 18-19 0.7817682254000573 2315 20-21 0.796366303490089 2315 22-23 0.8795283087209143 2315 24-25 0.8450144183592201 2315 26-27 0.9091437915342198 2315 28-29 0.9690797991671616 2315 30-31 0.9170464600173602 2315 32-33 0.9105191383538767 2315 34-35 0.8784783163702201 2315 36-37 0.9491730432528058 2315 38-39 1.026305038611433 2315 40-41 0.9163189004914116 2315 42-43 0.9875810140208188 2315 44-45 0.9409627434082708 2315 46-47 0.9279723251169898 2315 48-49 1.134555022896528 2315 50-51 0.9895172313691702 2315 52-53 0.9833279183664771 2315 54-55 0.9344072375313246 2315 56-57 1.0740205788748796 2315 58-59 1.0449565361656 2315 60-61 1.1483737760686168 2315 62-63 1.1034657358681699 2315 64-65 1.049787359586901 2315 66-67 1.0765553678999993 2315 68-69 0.9190210395636313 2315 70-71 0.5347597737636605 2315 72-73 0.8105507404687557 2315 74-75 0.9896414808656644 2315 76-77 0.9027020907260415 2315 78-79 0.8573554826658523 2315 80-81 0.9464421736593671 2315 82-83 0.9062740249022205 2315 84-85 0.82700383644735 2315 86-87 0.8421946119232864 2315 88-89 0.9060916494990252 2315 90-91 0.9402890185729795 2315 92-93 1.0461002491366251 2315 94-95 1.1292088718972444 2315 96-97 1.1689818680464583 2315 98-99 1.1289396515526846 2315 100 0.9894291992414672 2316 1 0.4927441723303261 2316 2 0.4850881052561178 2316 3 0.46020542987605495 2316 4 0.25098557158490564 2316 5 0.37660262312144965 2316 6 0.43854361505572825 2316 7 0.4594251631200237 2316 8 0.44412541671074734 2316 9 0.41698881107015495 2316 10-11 0.46618479441912797 2316 12-13 0.6293243849804426 2316 14-15 0.7854263318977956 2316 16-17 0.7352226278531546 2316 18-19 0.7623814225958228 2316 20-21 0.8306355372542953 2316 22-23 0.8300058572208755 2316 24-25 0.8634793366028006 2316 26-27 0.9951191753945139 2316 28-29 0.8830566999360769 2316 30-31 0.6930192999654423 2316 32-33 0.6396532044331522 2316 34-35 0.5598658140082762 2316 36-37 0.5392326631888764 2316 38-39 0.4481063072458369 2316 40-41 0.5220523277156417 2316 42-43 0.7173753355876613 2316 44-45 0.611365654449763 2316 46-47 0.6727749119026996 2316 48-49 0.4482974263281463 2316 50-51 0.49793008880789813 2316 52-53 0.6386708555205018 2316 54-55 0.7032846679748843 2316 56-57 0.7693644707901086 2316 58-59 0.6862137960263652 2316 60-61 0.7289424616074882 2316 62-63 0.7438215314410925 2316 64-65 0.7383960329207966 2316 66-67 0.7833373579817362 2316 68-69 0.6782880997032379 2316 70-71 0.6270313630290119 2316 72-73 0.6411070830871708 2316 74-75 0.6859355053856078 2316 76-77 0.6072554349223545 2316 78-79 0.6131113676523441 2316 80-81 0.5998946700241099 2316 82-83 0.4474517546071404 2316 84-85 0.5728640572295269 2316 86-87 0.5469031275037715 2316 88-89 0.5155439714292527 2316 90-91 0.6254087067835492 2316 92-93 0.6364992313176181 2316 94-95 0.5278092391717344 2316 96-97 0.7197533141883987 2316 98-99 0.81463201496965 2316 100 0.37070577849545217 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 3.0 12 25.0 13 135.0 14 532.0 15 1545.0 16 3641.0 17 7139.0 18 11575.0 19 16066.0 20 21592.0 21 28007.0 22 33032.0 23 33829.0 24 34561.0 25 37052.0 26 42851.0 27 51186.0 28 63217.0 29 77013.0 30 93311.0 31 116333.0 32 145169.0 33 188375.0 34 261127.0 35 359260.0 36 537349.0 37 818096.0 38 713358.0 39 103304.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.205269218572376 19.70204588626099 13.15574189320598 23.936943001960653 2 16.075766059536793 21.21674481196559 39.40207855701948 23.305410571478138 3 18.609032106569288 26.49669241499642 30.945801621768332 23.948473856665963 4 12.84545678823165 17.490655075458438 37.98887903295664 31.675009103353275 5 12.698988946996392 37.73037621280948 34.79243862404124 14.778196216152892 6 29.61771325032057 37.39367724635815 18.528793982561897 14.459815520759378 7 26.016363535850516 33.40280465107708 22.834529798430513 17.74630201464189 8 25.598049848894878 35.15244769235629 20.814783029894596 18.434719428854233 9 26.861788455225437 15.725794031988993 20.623784591189587 36.78863292159598 10-11 24.728105172724472 25.884274358982122 28.906454121848846 20.48116634644456 12-13 25.275108432288658 24.228048450463373 28.35820247222459 22.138640645023383 14-15 22.814961415003037 25.673221950242603 26.389823969648496 25.121992665105864 16-17 21.904620001187254 28.20464794503473 27.280237039642223 22.610495014135797 18-19 21.467252025894172 27.49972161477645 29.54484608258683 21.488180276742554 20-21 22.311753016103168 27.00297181162047 29.17342886092664 21.511846311349718 22-23 22.35951511198001 26.764163396790714 28.937398575272567 21.93892291595672 24-25 21.98065359628535 26.601877673371916 29.353997120849932 22.063471609492797 26-27 21.9297268823777 26.789938012890225 29.071657014662676 22.2086780900694 28-29 21.697161280234102 27.553611823572012 28.9950321811781 21.75419471501579 30-31 22.48716419497814 26.938678107217783 29.063313652226324 21.510844045577755 32-33 22.351452687572714 27.036357274443173 28.691826873331266 21.920363164652844 34-35 22.386716828671545 27.00762119822172 29.06919428683974 21.53646768626699 36-37 22.124298001527826 26.85536020451075 29.040939805680026 21.979401988281396 38-39 22.066359560818714 26.80170161091679 29.20522110660003 21.92671772166447 40-41 22.01819840286836 26.29477042713465 29.65975067687897 22.027280493118013 42-43 21.76092372079775 26.619995540505712 29.02036967470174 22.598711063994795 44-45 21.209502034252743 27.003874209239946 29.40118186352479 22.38544189298252 46-47 21.306713166522208 26.57425408383244 29.03113570087316 23.08789704877219 48-49 21.462940502670417 26.58193173941213 29.040959353929857 22.914168403987603 50-51 21.288705046095043 26.72688405990743 28.906231179870645 23.078179714126883 52-53 21.026899757969975 26.985682907098518 28.679458647883504 23.307958687048004 54-55 20.77915461854209 26.726143133694496 28.98693866138853 23.50776358637488 56-57 21.2373511744144 26.560572467457526 28.430524191446583 23.7715521666815 58-59 20.75511412169321 26.723001216703068 28.6801384643109 23.841746197292817 60-61 21.20192174800092 26.855427950903287 28.243509164500313 23.69914113659548 62-63 21.14124679738407 26.643237305860435 28.09670007983094 24.118815816924556 64-65 20.654045928715362 26.48398640416922 28.094364059991644 24.767603607123778 66-67 20.916418551181142 26.602632899257433 27.721582162548675 24.75936638701275 68-69 20.713896356569414 26.858732497740377 27.67144500177859 24.755926143911616 70-71 20.813925772582238 26.712164968546254 27.52344380663419 24.95046545223732 72-73 20.841827685165455 27.13978000758994 27.063722584235308 24.954669723009292 74-75 21.069572282472812 27.250312466138254 26.79743570399447 24.882679547394464 76-77 20.93445983576448 27.08308811234479 26.82221247921382 25.160239572676918 78-79 20.820515966375936 27.245097192588553 26.777537549049136 25.156849291986376 80-81 20.688373395826417 27.48702353257391 26.56925288665029 25.25535018494938 82-83 21.04386781671545 27.061966971031453 26.79161457285532 25.102550639397776 84-85 21.206657741708046 27.246846919927954 26.26812591408178 25.278369424282225 86-87 20.727505648452336 27.419853343698104 26.437785772445032 25.414855235404527 88-89 20.800592660882735 26.94699965428115 26.515950809146734 25.736456875689377 90-91 21.039431355901854 26.66314494399302 26.48356078816364 25.81386291194148 92-93 21.064183613698905 26.759236962569748 26.4248626895741 25.75171673415725 94-95 20.660044771376647 26.528199043336304 26.7245707347471 26.087185450539952 96-97 20.72297821946076 26.43219395603824 26.71678928138792 26.12803854311308 98-99 20.599250443244845 26.634647627094793 26.50494247460632 26.261159455054045 100 20.580327862809575 26.72033714666517 26.568204084738483 26.13113090578677 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2172.0 1 1536.0 2 1738.5 3 2583.5 4 1847.0 5 1244.5 6 1672.0 7 2767.0 8 4106.5 9 4685.0 10 3954.0 11 3092.5 12 3127.5 13 3456.0 14 3818.0 15 4231.0 16 4677.0 17 5124.0 18 5694.5 19 6307.5 20 6945.5 21 7745.5 22 8754.0 23 10176.0 24 11917.0 25 14098.5 26 17119.0 27 20709.0 28 24748.0 29 30286.5 30 37446.0 31 44359.5 32 52316.5 33 60440.0 34 67755.0 35 74911.5 36 83087.5 37 93830.0 38 100496.0 39 103024.5 40 105542.0 41 108249.5 42 112975.5 43 120361.0 44 129082.0 45 139275.5 46 148493.0 47 156972.0 48 164748.0 49 168466.5 50 170096.0 51 170009.5 52 165413.0 53 155790.5 54 144899.0 55 130021.0 56 113436.0 57 96522.0 58 79990.0 59 67288.5 60 53793.5 61 41218.0 62 32039.5 63 24449.5 64 17639.0 65 12283.0 66 8667.0 67 6480.5 68 4765.5 69 3279.0 70 2504.5 71 1956.5 72 1463.5 73 1106.5 74 821.0 75 588.0 76 399.5 77 248.0 78 155.5 79 101.0 80 71.0 81 39.0 82 25.0 83 18.0 84 11.0 85 8.0 86 6.5 87 4.5 88 2.5 89 1.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05267601251272477 2 0.0 3 0.0 4 0.016900549741713795 5 0.004685822202531239 6 0.0 7 7.897453150333549E-5 8 3.4222296984778714E-4 9 0.0019480384437489421 10-11 7.23933205447242E-4 12-13 2.3692359451000646E-4 14-15 3.9487265751667745E-5 16-17 9.213695342055807E-5 18-19 0.0 20-21 0.0 22-23 3.9487265751667745E-5 24-25 6.58121095861129E-5 26-27 0.0 28-29 0.0011582931287155871 30-31 0.0010924810191294742 32-33 0.013846867856918155 34-35 0.022770989916795065 36-37 0.030062971658936375 38-39 0.020072693423764436 40-41 2.105987506755613E-4 42-43 0.001474191254728929 44-45 0.009727029796827488 46-47 0.014347039889772614 48-49 0.005791465643577936 50-51 0.010516775111860843 52-53 0.016071317160928772 54-55 0.010108740032426943 56-57 0.0031326564162989746 58-59 0.008252838542098557 60-61 0.001790089380742271 62-63 0.001066156175295029 64-65 0.020112180689516104 66-67 0.04575257858426569 68-69 0.056532602134470984 70-71 0.04864831140605466 72-73 0.04111940606940334 74-75 0.028470318606952445 76-77 0.02890467853022079 78-79 0.012135753007679221 80-81 0.002263936569762284 82-83 0.027825359933008536 84-85 0.04870096109372355 86-87 0.048872072578647445 88-89 0.05937568526859107 90-91 0.03445922057928872 92-93 0.026390655944031276 94-95 0.007476255648982426 96-97 0.0037249654025739904 98-99 0.02233662999352672 100 0.018480040371780505 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3798693.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.03466158108976 #Duplication Level Percentage of deduplicated Percentage of total 1 79.25734802261054 52.33732154487741 2 13.21549658304639 17.453616889750336 3 3.5486010329504776 7.029920048915711 4 1.4855213366376923 3.9238359494543253 5 0.7350581559154185 2.4269658284147284 6 0.41536623816955914 1.6457141381842302 7 0.2933850181211965 1.356150626921657 8 0.19045490131706444 1.006129996394576 9 0.14224806294276165 0.8453972427291737 >10 0.6784007561878804 7.53462997359086 >50 0.02579027958040263 1.1588020324010895 >100 0.011127206323213514 1.3389681357364054 >500 6.680034461896913E-4 0.30592713373913083 >1k 4.008020633849932E-4 0.5951213452386129 >5k 4.453356259833257E-5 0.228144062675355 >10k+ 8.906712519666513E-5 0.8133550509764075 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14522 0.38228938216381264 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13136 0.3458031486092717 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7758 0.20422813846762555 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3914 0.10303543876801838 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.6324843834445164E-5 0.0 6 0.0 0.0 0.0 2.6324843834445164E-5 0.0 7 0.0 0.0 0.0 2.6324843834445164E-5 0.0 8 0.0 0.0 0.0 2.6324843834445164E-5 0.0 9 0.0 0.0 0.0 5.264968766889033E-5 2.6324843834445164E-5 10-11 0.0 0.0 0.0 5.264968766889033E-5 2.6324843834445164E-5 12-13 0.0 0.0 0.0 1.0529937533778065E-4 1.447866410894484E-4 14-15 0.0 0.0 0.0 1.5794906300667095E-4 1.7111148492389357E-4 16-17 0.0 0.0 0.0 2.105987506755613E-4 2.105987506755613E-4 18-19 0.0 0.0 0.0 3.290605479305645E-4 2.3692359451000646E-4 20-21 0.0 0.0 0.0 8.160701588678E-4 2.895732821788968E-4 22-23 0.0 0.0 0.0 0.0034617169642295388 3.4222296984778714E-4 24-25 0.0 0.0 0.0 0.009595405577655262 3.4222296984778714E-4 26-27 0.0 0.0 0.0 0.020572865456618895 3.553853917650097E-4 28-29 0.0 0.0 0.0 0.05114917157032695 3.6854781368223226E-4 30-31 0.0 0.0 0.0 0.14631348203184621 3.6854781368223226E-4 32-33 0.0 0.0 0.0 0.3621903638962138 3.6854781368223226E-4 34-35 0.0 0.0 0.0 0.6910798003418543 3.6854781368223226E-4 36-37 0.0 0.0 0.0 1.1685861426548554 3.6854781368223226E-4 38-39 0.0 0.0 0.0 1.8059764239963587 3.6854781368223226E-4 40-41 0.0 0.0 0.0 2.463334099386289 4.0803507943390006E-4 42-43 0.0 0.0 0.0 3.08803580599959 4.6068476710279036E-4 44-45 0.0 0.0 0.0 3.832436577528113 4.738471890200129E-4 46-47 0.0 0.0 0.0 4.683439804164221 4.738471890200129E-4 48-49 0.0 0.0 0.0 5.541945611293148 4.738471890200129E-4 50-51 0.0 0.0 0.0 6.383340269929684 5.001720328544581E-4 52-53 0.0 0.0 0.0 7.180548678190104 5.791465643577936E-4 54-55 0.0 0.0 0.0 8.037711918283472 6.054714081922388E-4 56-57 0.0 0.0 0.0 8.985169372728988 6.054714081922388E-4 58-59 0.0 0.0 0.0 10.003611768574086 6.054714081922388E-4 60-61 0.0 0.0 0.0 10.999270012080471 6.317962520266839E-4 62-63 0.0 0.0 0.0 11.983779684222968 7.107707835300194E-4 64-65 0.0 0.0 0.0 12.941424853232416 7.634204711989098E-4 66-67 0.0 0.0 0.0 13.98935107417209 7.897453150333549E-4 68-69 0.0 0.0 0.0 15.09151963583264 8.160701588678E-4 70-71 0.0 0.0 0.0 16.218209789524977 8.160701588678E-4 72-73 0.0 0.0 0.0 17.284273827866585 8.555574246194678E-4 74-75 0.0 0.0 0.0 18.308468202089507 8.687198465366904E-4 76-77 0.0 0.0 0.0 19.399988364419023 8.687198465366904E-4 78-79 0.0 0.0 0.0 20.546330014033774 8.687198465366904E-4 80-81 0.0 0.0 0.0 21.662042707847146 8.687198465366904E-4 82-83 0.0 0.0 0.0 22.759749208477757 8.950446903711356E-4 84-85 0.0 0.0 0.0 23.81684963749374 8.950446903711356E-4 86-87 1.3162421917222582E-5 0.0 0.0 24.885756758969467 9.082071122883582E-4 88 2.6324843834445164E-5 0.0 0.0 25.693258181169156 9.213695342055807E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6960 0.0 58.69765 1 TACCTGG 3030 0.0 41.5631 2 GTATCAA 12065 0.0 38.225384 1 GTGGTAT 2420 0.0 33.219334 1 ACCTGGG 3815 0.0 32.764877 3 ATAACGC 330 0.0 31.327822 3 ATCAACG 14630 0.0 30.995914 3 CAACGCA 14735 0.0 30.741125 5 TATCAAC 15170 0.0 30.07803 2 TCAACGC 15475 0.0 29.456402 4 AACGCAG 15965 0.0 28.753828 6 TGGTATC 2775 0.0 28.618013 2 GTACATG 11920 0.0 28.278284 1 TAACGCA 415 0.0 27.177015 4 TACATGG 12165 0.0 26.923801 2 ACATGGG 12180 0.0 25.965052 3 ACGCAGA 17620 0.0 25.759726 7 CCTGGGG 3550 0.0 25.681004 4 CGCAGAG 17925 0.0 25.268991 8 TGGGGGG 7310 0.0 24.682198 6 >>END_MODULE