FastQCFastQC Report
Mon 6 Jun 2016
ERR1211461_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211461_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3994294
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169590.4245806643176492No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141110.3532789524256352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75570.18919488650560023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68000.057.7166561
GTATCAA127850.043.2645761
GTGGTAT23250.036.273971
ATCAACG156650.034.7819633
TACCTGG29700.034.7121962
CAACGCA157800.034.5582735
TATCAAC161550.034.093642
TCAACGC167250.032.773484
AACGCAG170200.032.4824526
ACGCAGA182450.029.7092677
CGCAGAG185700.029.08558
GTACTAG14900.028.775321
GTACATG132200.028.689931
TACATGG134950.027.6278292
ACCTGGG36700.027.5406043
TGGTATC31900.026.8580932
ACATGGG134700.026.4197883
GCAGAGT197150.026.2746499
CATGGGG103000.024.3265294
GAGTACT133050.022.80274812-13
Produced by FastQC (version 0.11.5)