##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211461_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3994294 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.333684500940592 31.0 31.0 34.0 28.0 34.0 2 31.379216702626298 31.0 31.0 34.0 28.0 34.0 3 31.436004460362707 31.0 31.0 34.0 28.0 34.0 4 35.08286570793236 37.0 35.0 37.0 32.0 37.0 5 35.13823218821649 37.0 35.0 37.0 32.0 37.0 6 35.20661248270658 37.0 35.0 37.0 32.0 37.0 7 35.13838465571137 37.0 35.0 37.0 32.0 37.0 8 35.144560715861175 37.0 35.0 37.0 32.0 37.0 9 36.71016830508721 39.0 37.0 39.0 32.0 39.0 10-11 36.639460565496684 39.0 37.0 39.0 32.0 39.0 12-13 36.546666820219045 39.0 37.0 39.0 32.0 39.0 14-15 37.71625373595434 40.0 37.0 41.0 32.0 41.0 16-17 37.70827435336508 40.0 37.0 41.0 32.0 41.0 18-19 37.709871005990045 40.0 37.0 41.0 32.0 41.0 20-21 37.698375607804536 40.0 37.0 41.0 32.0 41.0 22-23 37.569985834793336 40.0 37.0 41.0 32.0 41.0 24-25 37.47331330643162 40.0 37.0 41.0 31.5 41.0 26-27 37.32922689216167 40.0 36.5 41.0 31.0 41.0 28-29 37.19883376136058 40.0 36.0 41.0 31.0 41.0 30-31 37.03101336556598 39.0 36.0 41.0 30.5 41.0 32-33 36.8259221779869 39.0 35.5 41.0 30.0 41.0 34-35 36.691449102144205 39.0 35.0 41.0 30.0 41.0 36-37 36.54437955743869 39.0 35.0 41.0 30.0 41.0 38-39 36.38443414530828 39.0 35.0 40.0 29.5 41.0 40-41 36.211911166278696 39.0 35.0 40.0 29.0 41.0 42-43 35.999892847146455 38.0 35.0 40.0 28.0 41.0 44-45 35.83614087996527 38.0 35.0 40.0 27.5 41.0 46-47 35.59115828729683 38.0 34.0 40.0 26.5 41.0 48-49 35.32290186951687 38.0 34.0 40.0 26.0 41.0 50-51 34.63488866868588 37.5 33.5 39.5 25.0 40.5 52-53 34.923206579185205 38.0 33.5 39.5 26.0 40.5 54-55 35.321817697946116 38.0 34.0 40.0 26.0 41.0 56-57 35.23162466258117 38.0 34.0 40.0 26.0 41.0 58-59 35.00719238493711 38.0 34.0 40.0 26.0 41.0 60-61 34.77096440572477 37.0 34.0 40.0 26.0 41.0 62-63 34.47963332193374 37.0 33.0 40.0 25.0 41.0 64-65 34.2627906208206 36.0 33.0 40.0 25.0 41.0 66-67 34.056283538467625 36.0 33.0 39.0 25.0 41.0 68-69 33.70843833728814 35.5 33.0 39.0 24.0 41.0 70-71 33.3357970144411 35.0 32.0 39.0 23.5 40.5 72-73 32.95673252895256 35.0 32.0 37.5 22.5 40.0 74-75 32.57334913253756 35.0 32.0 37.0 22.0 39.5 76-77 32.19159067910374 35.0 31.0 36.5 20.5 39.0 78-79 31.79556036686333 35.0 31.0 36.0 20.0 39.0 80-81 31.400466640662906 35.0 31.0 36.0 19.5 37.5 82-83 31.03500092882497 34.5 30.0 35.0 18.0 37.0 84-85 30.706928558588828 34.0 30.0 35.0 18.0 37.0 86-87 30.367405854451377 34.0 29.0 35.0 16.5 36.0 88-89 30.047121844310908 34.0 29.0 35.0 14.5 36.0 90-91 29.73492987747021 34.0 29.0 35.0 11.5 35.5 92-93 29.434226549172394 34.0 28.5 35.0 10.0 35.0 94-95 29.14558142189834 34.0 27.0 35.0 10.0 35.0 96-97 28.845397709833076 34.0 26.5 35.0 10.0 35.0 98-99 28.51767846833508 33.5 25.5 35.0 9.0 35.0 100 27.240648034421103 32.0 24.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3648255086308474 1101 2 -0.31355042785668985 1101 3 -0.3745281227915527 1101 4 -0.30820408823917944 1101 5 -0.1921625237961493 1101 6 -0.25034252440814697 1101 7 -0.1844981908514356 1101 8 -0.2732632194595581 1101 9 -0.4636828842234664 1101 10-11 -0.4624723960376613 1101 12-13 -0.4033359865345929 1101 14-15 -0.5813937730290206 1101 16-17 -0.5563777631126499 1101 18-19 -0.588549275219691 1101 20-21 -0.5864907685737109 1101 22-23 -0.5617963360061253 1101 24-25 -0.6021919021000599 1101 26-27 -0.6602613466339946 1101 28-29 -0.6965099986896846 1101 30-31 -0.7568023581469916 1101 32-33 -0.6814416019555907 1101 34-35 -0.7680829268949125 1101 36-37 -0.8346334691294786 1101 38-39 -0.8313011455004542 1101 40-41 -1.0474915832574396 1101 42-43 -0.9846022877559406 1101 44-45 -1.0467399369706598 1101 46-47 -1.0304195791465958 1101 48-49 -1.107465813392544 1101 50-51 -1.0330651687201922 1101 52-53 -0.8812015214820121 1101 54-55 -0.8286260405862862 1101 56-57 -0.8195100476649699 1101 58-59 -0.8274016383711427 1101 60-61 -0.9030420563749999 1101 62-63 -0.901735573345654 1101 64-65 -0.8760899399098747 1101 66-67 -0.9383182608936025 1101 68-69 -0.840254391353632 1101 70-71 -0.8430275359707977 1101 72-73 -0.8985291557278714 1101 74-75 -1.1343852517079611 1101 76-77 -0.9910548373467591 1101 78-79 -0.9987142228241517 1101 80-81 -1.3195777369809498 1101 82-83 -1.2462632612691031 1101 84-85 -1.3601684546331292 1101 86-87 -1.4463353872096718 1101 88-89 -1.5680889785802634 1101 90-91 -1.6763175549701081 1101 92-93 -1.6524536941329124 1101 94-95 -1.6411714877215218 1101 96-97 -1.8005701602517554 1101 98-99 -1.8765050690426683 1101 100 -1.85702838429701 1102 1 -0.4205644549589742 1102 2 -0.493852342712529 1102 3 -0.514447484693001 1102 4 -0.3779143069842803 1102 5 -0.3265275128519889 1102 6 -0.31083683920846283 1102 7 -0.512549478984603 1102 8 -0.45027667768705726 1102 9 -0.5072280438657728 1102 10-11 -0.425783048860886 1102 12-13 -0.5180380605277222 1102 14-15 -0.7494633977531393 1102 16-17 -0.6763142609765538 1102 18-19 -0.7386098583470542 1102 20-21 -0.7835589279292137 1102 22-23 -0.8085406536035364 1102 24-25 -0.7435236228668956 1102 26-27 -0.7760236157107343 1102 28-29 -0.7957889348587628 1102 30-31 -0.7633008756099997 1102 32-33 -0.7875629245389248 1102 34-35 -0.9702095053915158 1102 36-37 -1.0248560398803264 1102 38-39 -0.9656246604036482 1102 40-41 -1.074696351141462 1102 42-43 -1.0457344943143525 1102 44-45 -1.1674534868425397 1102 46-47 -1.2475069840521513 1102 48-49 -1.2933297400542045 1102 50-51 -1.0988300188395428 1102 52-53 -0.7548521936835044 1102 54-55 -0.9500397513161474 1102 56-57 -0.8980144071578309 1102 58-59 -0.8490814704127843 1102 60-61 -1.0486304526864245 1102 62-63 -0.8532258611793608 1102 64-65 -1.0497877421810173 1102 66-67 -1.075273821975614 1102 68-69 -1.0616417978255654 1102 70-71 -0.9857283667424426 1102 72-73 -0.9650787353960197 1102 74-75 -1.1571180642596843 1102 76-77 -1.123348009219736 1102 78-79 -1.1668601985697613 1102 80-81 -1.3363853398914394 1102 82-83 -1.4155066623841925 1102 84-85 -1.4402600974640514 1102 86-87 -1.5263739045445917 1102 88-89 -1.5875175164547315 1102 90-91 -1.6934588415515641 1102 92-93 -1.8329636356223382 1102 94-95 -1.856094983535023 1102 96-97 -1.9922205992720556 1102 98-99 -1.9625614666371192 1102 100 -1.9266664573067978 1103 1 -0.3952892396133727 1103 2 -0.4932618541485567 1103 3 -0.5460941513649296 1103 4 -0.4138104233044473 1103 5 -0.3007269276518585 1103 6 -0.4055220474738803 1103 7 -0.40878948523599945 1103 8 -0.33327359066556994 1103 9 -0.6020162580897548 1103 10-11 -0.5973111363362236 1103 12-13 -0.575173808173389 1103 14-15 -0.6535075064042957 1103 16-17 -0.7494774200469578 1103 18-19 -0.8385230334011453 1103 20-21 -0.749006703908222 1103 22-23 -0.7332139747445368 1103 24-25 -0.7644556570885541 1103 26-27 -0.7309043221816367 1103 28-29 -0.8618110578549789 1103 30-31 -0.8712393593465038 1103 32-33 -0.947497790043137 1103 34-35 -0.852875812655526 1103 36-37 -0.8435301124517807 1103 38-39 -0.8279989911609178 1103 40-41 -0.8437595573341312 1103 42-43 -0.9371091463239196 1103 44-45 -0.8756803872630101 1103 46-47 -0.9638442816020287 1103 48-49 -0.8965247402002277 1103 50-51 -0.8898503066721304 1103 52-53 -0.6714682387756383 1103 54-55 -0.7309317118786751 1103 56-57 -0.7523702568306945 1103 58-59 -0.7495304343149201 1103 60-61 -0.744348483149345 1103 62-63 -0.7206615337609108 1103 64-65 -0.6212871379583262 1103 66-67 -0.7740472617510719 1103 68-69 -0.8086591241381811 1103 70-71 -0.8674663180052278 1103 72-73 -0.837043986474022 1103 74-75 -0.9477142911632583 1103 76-77 -0.9714349004683314 1103 78-79 -0.9014575715626236 1103 80-81 -0.9252514613500686 1103 82-83 -1.092240339491692 1103 84-85 -1.2806340919719794 1103 86-87 -1.3463407610947833 1103 88-89 -1.4180574413274698 1103 90-91 -1.4910758125133547 1103 92-93 -1.4774970186300642 1103 94-95 -1.4767328390535788 1103 96-97 -1.6739671146317825 1103 98-99 -1.746990122973724 1103 100 -1.709531791026368 1104 1 -0.47134028672166295 1104 2 -0.35518937368047077 1104 3 -0.38585800751613064 1104 4 -0.41479142120065404 1104 5 -0.35824945615809867 1104 6 -0.2594817201347652 1104 7 -0.24906847726877146 1104 8 -0.41662628342833585 1104 9 -0.3594377674456055 1104 10-11 -0.4833674815436879 1104 12-13 -0.4890657949142465 1104 14-15 -0.66061074365102 1104 16-17 -0.6091899325198895 1104 18-19 -0.729667252912364 1104 20-21 -0.7656282157355037 1104 22-23 -0.5922349539815528 1104 24-25 -0.5874494847257878 1104 26-27 -0.5941386804320032 1104 28-29 -0.7292279796439161 1104 30-31 -0.7933417007047368 1104 32-33 -0.8167016232101645 1104 34-35 -0.6995214053475465 1104 36-37 -0.796094979412338 1104 38-39 -0.7389859822421911 1104 40-41 -0.7535258231164406 1104 42-43 -0.8320048988856925 1104 44-45 -0.8481956096441579 1104 46-47 -0.8035344586481301 1104 48-49 -0.867828891253204 1104 50-51 -0.702810723847243 1104 52-53 -0.6440232355164639 1104 54-55 -0.5751814207149195 1104 56-57 -0.5840104896381391 1104 58-59 -0.6011437286822456 1104 60-61 -0.6070242240336796 1104 62-63 -0.6502829281958995 1104 64-65 -0.6663846198938259 1104 66-67 -0.5759718001368057 1104 68-69 -0.6910166855914994 1104 70-71 -0.5469876200710289 1104 72-73 -0.6103643500193954 1104 74-75 -0.6640766904144222 1104 76-77 -0.786421261338397 1104 78-79 -0.7529198005949702 1104 80-81 -0.6198419167394569 1104 82-83 -0.7399354346566724 1104 84-85 -0.7495608947931025 1104 86-87 -0.7747628234300876 1104 88-89 -0.7905900408560456 1104 90-91 -0.8797149424511659 1104 92-93 -0.9852401982927752 1104 94-95 -1.1222480676132705 1104 96-97 -1.1733766777780197 1104 98-99 -1.2094048977195548 1104 100 -1.1536841106052407 1105 1 -0.4769606663628494 1105 2 -0.4916529590417156 1105 3 -0.470730381843115 1105 4 -0.2964186652686678 1105 5 -0.3909379043126364 1105 6 -0.34606172105716126 1105 7 -0.3226067367036407 1105 8 -0.41744937491981204 1105 9 -0.47845017667494716 1105 10-11 -0.4729351967190709 1105 12-13 -0.4952254798706761 1105 14-15 -0.728722289843077 1105 16-17 -0.7184711166409556 1105 18-19 -0.6803805864738166 1105 20-21 -0.755261800546279 1105 22-23 -0.6967023360522191 1105 24-25 -0.6662135382784342 1105 26-27 -0.7661968257275475 1105 28-29 -0.70369276561037 1105 30-31 -0.7101515870266084 1105 32-33 -0.7090126171535829 1105 34-35 -0.744104957502465 1105 36-37 -0.7882205267142481 1105 38-39 -0.8889012555174887 1105 40-41 -0.8413840892163833 1105 42-43 -0.6535246687027438 1105 44-45 -0.67558763328136 1105 46-47 -0.7788526487841239 1105 48-49 -0.8959372983163973 1105 50-51 -0.9887811237960662 1105 52-53 -0.6929993364803693 1105 54-55 -0.734285154790534 1105 56-57 -0.7676234558768797 1105 58-59 -0.6830424478879138 1105 60-61 -0.6339994576458707 1105 62-63 -0.7252810685223992 1105 64-65 -0.8325617709037729 1105 66-67 -0.7043258328759805 1105 68-69 -0.8201600381513785 1105 70-71 -0.7583037705171236 1105 72-73 -0.7816656082001003 1105 74-75 -0.7136391836266114 1105 76-77 -0.8635438554787882 1105 78-79 -0.9452018952121826 1105 80-81 -0.8845112072814523 1105 82-83 -0.8447081751161782 1105 84-85 -0.9760404510303715 1105 86-87 -0.9955361203039033 1105 88-89 -1.0247258728962905 1105 90-91 -1.0885328075162342 1105 92-93 -1.0031307408859966 1105 94-95 -1.0719979415274068 1105 96-97 -1.3520769817230587 1105 98-99 -1.2843154846176326 1105 100 -1.1591953646053987 1106 1 -0.6118140353153869 1106 2 -0.585324221156398 1106 3 -0.5649504928641065 1106 4 -0.379751212023443 1106 5 -0.47132776922966 1106 6 -0.5573043798097501 1106 7 -0.5473985952131528 1106 8 -0.4974330726219378 1106 9 -0.5982856024901579 1106 10-11 -0.8366162307003648 1106 12-13 -0.6757029659802427 1106 14-15 -0.825100077303702 1106 16-17 -0.7354125187336962 1106 18-19 -0.7766211800003759 1106 20-21 -0.905796948351707 1106 22-23 -0.762365438237552 1106 24-25 -0.7300806072342994 1106 26-27 -0.8933539076601349 1106 28-29 -0.8865337922307504 1106 30-31 -0.883973484422377 1106 32-33 -1.0418283452125152 1106 34-35 -0.9463416576481123 1106 36-37 -1.0369953117130635 1106 38-39 -1.0576319043130908 1106 40-41 -0.9394529474006603 1106 42-43 -0.8862135929815764 1106 44-45 -0.9271803451005596 1106 46-47 -1.0784954689499529 1106 48-49 -1.0764589059951604 1106 50-51 -0.9722330475659859 1106 52-53 -1.012143195112813 1106 54-55 -0.8831257928776921 1106 56-57 -0.891650357595168 1106 58-59 -0.7972028008897354 1106 60-61 -0.8260536240061356 1106 62-63 -0.9532721798188746 1106 64-65 -0.8125905048013209 1106 66-67 -0.979567998462116 1106 68-69 -0.9757280814036804 1106 70-71 -0.8964651230224874 1106 72-73 -0.965914025073566 1106 74-75 -1.0939114276108413 1106 76-77 -1.025814060212383 1106 78-79 -1.0153631291819885 1106 80-81 -0.997397120098551 1106 82-83 -0.9950513977541284 1106 84-85 -0.9825633091861121 1106 86-87 -1.0769276472797031 1106 88-89 -1.0637126572790088 1106 90-91 -1.0775905120165952 1106 92-93 -1.040867340971534 1106 94-95 -1.0875800992916496 1106 96-97 -1.123440729671092 1106 98-99 -1.126059464639269 1106 100 -1.1981890269773459 1107 1 -0.48005489933095546 1107 2 -0.4984023059574305 1107 3 -0.5043959731941676 1107 4 -0.359032462710573 1107 5 -0.34810837954363194 1107 6 -0.3501344204973975 1107 7 -0.4977972668285986 1107 8 -0.49272446520783575 1107 9 -0.6028194373472004 1107 10-11 -0.5588390067460836 1107 12-13 -0.6076697433574125 1107 14-15 -0.8118452898117852 1107 16-17 -0.8026654158256719 1107 18-19 -0.7708794914358919 1107 20-21 -0.8701289590838144 1107 22-23 -0.8457177370799585 1107 24-25 -0.8338435409868055 1107 26-27 -0.8383923219187395 1107 28-29 -0.9460150373042282 1107 30-31 -0.9132530135432546 1107 32-33 -0.7927562764901381 1107 34-35 -0.8008624870796339 1107 36-37 -0.8136258382146835 1107 38-39 -0.9633523967559796 1107 40-41 -0.9880747151953742 1107 42-43 -1.0116564897603482 1107 44-45 -0.8736837167898202 1107 46-47 -0.7881424333337463 1107 48-49 -0.822335966934439 1107 50-51 -0.796870880578588 1107 52-53 -0.7432695441473243 1107 54-55 -0.7745048961177261 1107 56-57 -0.6687551809006109 1107 58-59 -0.6871046447472864 1107 60-61 -0.6144476444939713 1107 62-63 -0.6501051413009691 1107 64-65 -0.596878748865258 1107 66-67 -0.5230065792354139 1107 68-69 -0.4387275220890814 1107 70-71 -0.5887565432524227 1107 72-73 -0.5313689282745244 1107 74-75 -0.4869242866148511 1107 76-77 -0.3839000871959364 1107 78-79 -0.5101274114122347 1107 80-81 -0.6411426699205016 1107 82-83 -0.6786812187317395 1107 84-85 -0.7208723719703976 1107 86-87 -0.7003919094379754 1107 88-89 -0.7512651394204966 1107 90-91 -0.7188761663782692 1107 92-93 -0.804459389300952 1107 94-95 -0.9221724030633212 1107 96-97 -0.9443076255924119 1107 98-99 -0.8252399778950839 1107 100 -0.7120070529708045 1108 1 -0.5301254097182486 1108 2 -0.490782211317633 1108 3 -0.5679495657021008 1108 4 -0.4465583894796481 1108 5 -0.3568925066098174 1108 6 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58-59 1.0535478022066016 2311 60-61 1.0154750376583053 2311 62-63 0.9618726381868967 2311 64-65 0.9457531638951195 2311 66-67 1.0203176131582552 2311 68-69 0.9848376832959147 2311 70-71 1.0823180084215736 2311 72-73 1.0887001246672625 2311 74-75 1.0588120359381747 2311 76-77 0.9971185492640515 2311 78-79 1.078962551602661 2311 80-81 1.0381452257844437 2311 82-83 1.1717951593336728 2311 84-85 1.1878635363649899 2311 86-87 1.1812232170900927 2311 88-89 1.275418171886976 2311 90-91 1.3020497975667347 2311 92-93 1.3543199827162162 2311 94-95 1.4418510587126718 2311 96-97 1.4558634510150732 2311 98-99 1.6360073367050205 2311 100 1.863460205916887 2312 1 0.6799695400080132 2312 2 0.5692526474027702 2312 3 0.7039137926373478 2312 4 0.4963330183970669 2312 5 0.5259793091393661 2312 6 0.43580565011443895 2312 7 0.5196956085182194 2312 8 0.5669065733106251 2312 9 0.7510925447640915 2312 10-11 0.8491065682967758 2312 12-13 0.8463863638966913 2312 14-15 1.06582025113876 2312 16-17 1.0450905311498886 2312 18-19 1.0129526024937547 2312 20-21 1.090596995298874 2312 22-23 1.0783268619237774 2312 24-25 1.0703608064814532 2312 26-27 1.0954140074794623 2312 28-29 1.145410939536987 2312 30-31 1.135751962205724 2312 32-33 1.2600547078209772 2312 34-35 1.262379519568647 2312 36-37 1.2676523447929995 2312 38-39 1.3296226045343431 2312 40-41 1.3009416432594207 2312 42-43 1.3267636297301664 2312 44-45 1.406731891466542 2312 46-47 1.3502936442639424 2312 48-49 1.3466189862862734 2312 50-51 1.2987527480215704 2312 52-53 1.1363832833251664 2312 54-55 1.1551713802867383 2312 56-57 1.1105706727674587 2312 58-59 1.1560243718064598 2312 60-61 1.1924814102811112 2312 62-63 1.2360430542751146 2312 64-65 1.2322041760995575 2312 66-67 1.2319879316778923 2312 68-69 1.2631335224136748 2312 70-71 1.295883053219498 2312 72-73 1.2350536797879457 2312 74-75 1.1976792367231823 2312 76-77 1.2064960616552654 2312 78-79 1.1637692821628143 2312 80-81 1.0662736372005277 2312 82-83 1.2675878901948927 2312 84-85 1.292222900998329 2312 86-87 1.485715386513771 2312 88-89 1.4593196889391749 2312 90-91 1.5228297887576865 2312 92-93 1.5599282253107596 2312 94-95 1.652551601194034 2312 96-97 1.7478226840701971 2312 98-99 1.6647747945135727 2312 100 1.7426174363069933 2313 1 0.7298588312382712 2313 2 0.6807322253336991 2313 3 0.7147876953918733 2313 4 0.6221772214366936 2313 5 0.618792713596271 2313 6 0.677518888875916 2313 7 0.726039882940988 2313 8 0.7049569481802962 2313 9 0.8633005336196717 2313 10-11 0.8153642960630876 2313 12-13 0.9183749469196201 2313 14-15 1.1027901663874928 2313 16-17 1.068005537617637 2313 18-19 1.1164410751972724 2313 20-21 1.1223300915536356 2313 22-23 1.1639313906435902 2313 24-25 1.229489304796374 2313 26-27 1.2682868762599355 2313 28-29 1.2214432696126494 2313 30-31 1.2591233881814006 2313 32-33 1.2687389866565226 2313 34-35 1.2485933142534122 2313 36-37 1.3064547735504632 2313 38-39 1.3882230189892084 2313 40-41 1.4369325711105247 2313 42-43 1.484622697769126 2313 44-45 1.4054513210935795 2313 46-47 1.452965147679521 2313 48-49 1.5091259839976843 2313 50-51 1.3706272261675139 2313 52-53 1.3451311580542367 2313 54-55 1.2985911953827625 2313 56-57 1.2599471301733516 2313 58-59 1.2606291254913486 2313 60-61 1.211170036999178 2313 62-63 1.1700807662073487 2313 64-65 1.1671226262632999 2313 66-67 1.205580169517873 2313 68-69 1.254432609142036 2313 70-71 1.2147184480115527 2313 72-73 1.3457266930652096 2313 74-75 1.3343467253775927 2313 76-77 1.328042653218212 2313 78-79 1.345355285874735 2313 80-81 1.4245741775493137 2313 82-83 1.4325449954136538 2313 84-85 1.5446766425567269 2313 86-87 1.5930266862494378 2313 88-89 1.6481859947964885 2313 90-91 1.7548987102665095 2313 92-93 1.8208894318157114 2313 94-95 1.9704505140070907 2313 96-97 1.9653474626742877 2313 98-99 2.074078095010659 2313 100 2.115344575313774 2314 1 0.5393715947844413 2314 2 0.5035534159631077 2314 3 0.5824772098696513 2314 4 0.46442297429068446 2314 5 0.5261519050773558 2314 6 0.5489557350698462 2314 7 0.5424735183510379 2314 8 0.5181323522152752 2314 9 0.702455396800822 2314 10-11 0.7175387008096052 2314 12-13 0.5347680733932165 2314 14-15 0.8961593024553451 2314 16-17 0.952354394728232 2314 18-19 0.9556404948072696 2314 20-21 0.9525900800165843 2314 22-23 0.7948746570447796 2314 24-25 0.9169592887288545 2314 26-27 1.0285482898483522 2314 28-29 0.9945880385518535 2314 30-31 0.9771717519740974 2314 32-33 1.007658040890199 2314 34-35 1.0417458754657858 2314 36-37 1.01219323343539 2314 38-39 1.1350783044700776 2314 40-41 1.1502194342427003 2314 42-43 1.2757495570646782 2314 44-45 1.296328375281 2314 46-47 1.2668988613184098 2314 48-49 1.3004486704986462 2314 50-51 1.1924629884497833 2314 52-53 1.0084342961873318 2314 54-55 1.0787849784344488 2314 56-57 0.9941463491093501 2314 58-59 1.0582460878176931 2314 60-61 1.058514014670621 2314 62-63 0.9909213215369164 2314 64-65 1.0118429615453337 2314 66-67 1.1009544469214418 2314 68-69 1.0918342108606183 2314 70-71 1.144464694047393 2314 72-73 1.1021486009510824 2314 74-75 1.1644766158708109 2314 76-77 1.1358201819486027 2314 78-79 1.2113633053516786 2314 80-81 1.2484860653405754 2314 82-83 1.1796768028536952 2314 84-85 1.2955884337523784 2314 86-87 1.5822402040577224 2314 88-89 1.6737346851095793 2314 90-91 1.6487044993370539 2314 92-93 1.7610490424673522 2314 94-95 1.8317139540627103 2314 96-97 1.9588848247704398 2314 98-99 1.9422120683693187 2314 100 1.827497970513651 2315 1 0.442190668205221 2315 2 0.38931269006974745 2315 3 0.4775397669363386 2315 4 0.37372602114178477 2315 5 0.40430210443763315 2315 6 -0.37622171767210233 2315 7 0.2699474595120108 2315 8 0.40268835607801634 2315 9 0.5441575416482678 2315 10-11 0.6593840752497329 2315 12-13 0.7273802748966958 2315 14-15 0.8744222480561774 2315 16-17 0.7992942793653981 2315 18-19 0.8662463149603852 2315 20-21 0.9009686313060286 2315 22-23 0.8651465204807138 2315 24-25 0.8929123087815825 2315 26-27 1.0319356943802305 2315 28-29 1.0517033209398505 2315 30-31 1.0376546647692848 2315 32-33 1.043088760958419 2315 34-35 1.0666708434139878 2315 36-37 0.9946520669964585 2315 38-39 1.031129420202923 2315 40-41 1.1542510367512548 2315 42-43 1.1669135969630418 2315 44-45 1.1970496628303309 2315 46-47 1.1372774923789422 2315 48-49 1.2156159047824815 2315 50-51 1.086101871782617 2315 52-53 0.9757972720438417 2315 54-55 0.8522940515955 2315 56-57 0.7675689684480957 2315 58-59 0.7557985950755182 2315 60-61 0.7354146200539731 2315 62-63 0.8670664288703591 2315 64-65 0.9552561626666503 2315 66-67 0.965596916411954 2315 68-69 1.0819166019224795 2315 70-71 1.0488107177871413 2315 72-73 1.0654702137236356 2315 74-75 1.0312655687040078 2315 76-77 1.0155044781398601 2315 78-79 1.0013240075464473 2315 80-81 1.1016515316743032 2315 82-83 1.3460837988631162 2315 84-85 1.2302472419823296 2315 86-87 1.3263033498789483 2315 88-89 1.4917194328086154 2315 90-91 1.5479562827185624 2315 92-93 1.6974803121634743 2315 94-95 1.7783269163429551 2315 96-97 1.8492813840896112 2315 98-99 2.005395814595367 2315 100 1.8839815138883829 2316 1 0.08126698652284858 2316 2 0.04400765085342684 2316 3 0.16810518900265592 2316 4 0.08600865676835667 2316 5 0.07662113108553825 2316 6 0.1814049139745677 2316 7 0.16582397290745376 2316 8 0.23123599590091715 2316 9 0.4481617879202915 2316 10-11 0.30673711810123905 2316 12-13 0.3728275877292262 2316 14-15 0.5741156272602197 2316 16-17 0.6586953160590241 2316 18-19 0.5708223977548528 2316 20-21 0.49006789476977275 2316 22-23 0.3820351665016446 2316 24-25 0.42253932039909614 2316 26-27 0.5403737498305077 2316 28-29 0.6025952045473417 2316 30-31 0.6720114515086877 2316 32-33 0.7398476684834989 2316 34-35 0.7931915738589552 2316 36-37 0.8464607183839874 2316 38-39 0.9823854294710728 2316 40-41 0.9665593106162191 2316 42-43 0.9180771793045892 2316 44-45 0.8146637985288905 2316 46-47 0.7845839594238271 2316 48-49 0.9211301434061241 2316 50-51 0.708129329572067 2316 52-53 0.5359830072727192 2316 54-55 0.4203151576044277 2316 56-57 0.585288869224776 2316 58-59 0.6547867366557796 2316 60-61 0.7038547712196959 2316 62-63 0.7012374412086331 2316 64-65 0.7706628832539124 2316 66-67 0.7588570368078678 2316 68-69 0.757083372885262 2316 70-71 0.6394119237048912 2316 72-73 0.762962582479453 2316 74-75 0.9069914060353526 2316 76-77 1.064241633313877 2316 78-79 1.060111587200769 2316 80-81 1.1206099611020655 2316 82-83 1.224482300132781 2316 84-85 1.285524028212496 2316 86-87 1.4523636496837753 2316 88-89 1.5193610968066729 2316 90-91 1.5503614197675795 2316 92-93 1.6905562446344327 2316 94-95 1.8160942743019248 2316 96-97 1.8320646900513324 2316 98-99 2.023463545376238 2316 100 1.9631925455777548 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 19.0 1 229.0 2 102.0 3 69.0 4 63.0 5 62.0 6 61.0 7 368.0 8 1314.0 9 2419.0 10 3221.0 11 4029.0 12 5068.0 13 6057.0 14 7545.0 15 9791.0 16 13116.0 17 17720.0 18 22370.0 19 27488.0 20 34451.0 21 40859.0 22 44602.0 23 45252.0 24 45585.0 25 47526.0 26 53248.0 27 61832.0 28 72891.0 29 86859.0 30 104196.0 31 127671.0 32 159594.0 33 204553.0 34 277732.0 35 376321.0 36 533904.0 37 739685.0 38 649089.0 39 167134.0 40 199.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.885365446095996 19.15733604441164 12.373325920019509 23.58397258947286 2 16.451608454902964 20.877933936825162 39.499004000295855 23.17145360797602 3 19.17184896486755 26.480404427102506 30.093338962618837 24.254407645411106 4 12.931950427540057 17.08444902085352 38.27627248476058 31.707328066845847 5 13.010531482383463 37.51654348008459 34.794560340974115 14.67836469655783 6 29.62589916884573 37.16452676358833 18.73034115124172 14.479232916324225 7 26.163006250663845 33.14044253657911 23.012122560307184 17.684428652449864 8 25.588577434616983 34.530210151476595 21.072810563082516 18.808401850823913 9 26.90987388896458 15.582447042982745 20.856333803528063 36.65134526452461 10-11 24.727755075307623 25.788883182485158 28.820486348399616 20.662875393807603 12-13 25.42612323592932 24.170088133718604 28.251003147507582 22.152785482844493 14-15 22.95496639704758 25.50219752303352 26.36067323346455 25.182162846454347 16-17 21.939795905847152 28.052604337645537 27.21410204306996 22.793497713437347 18-19 21.544328086522118 27.424019395185034 29.417286820045778 21.614365698247067 20-21 22.490959569034185 26.877017835960842 29.170173451570307 21.461849143434662 22-23 22.58400036460926 26.51758588601553 28.93174494580444 21.966668803570766 24-25 22.181867262174997 26.514085185970732 29.246383641617236 22.057663910237036 26-27 22.020552408742454 26.732354312138344 29.04027785828962 22.20681542082958 28-29 21.766374382676055 27.332791208818723 28.934737848992658 21.966096559512565 30-31 22.46364355124145 26.86189901761999 29.01030206322964 21.66415536790892 32-33 22.373601293070255 27.020450448521537 28.579642357241337 22.02630590116687 34-35 22.48052493615785 26.901488074555857 28.965559985951284 21.652427003335003 36-37 22.270719940700896 26.58811037947169 28.98027077753696 22.16089890229045 38-39 22.1421369333233 26.737190023041872 29.100713267690555 22.019959775944272 40-41 22.0876379629865 26.20613415907765 29.498477021600912 22.207750856334936 42-43 21.804143068167402 26.52181442329256 28.967067845849726 22.70697466269031 44-45 21.443078180749875 26.86394144145272 29.225626026555318 22.467354351242086 46-47 21.50242514486374 26.509807654015173 28.958746864468655 23.029020336652437 48-49 21.67015197828742 26.495735914605334 28.970572231857144 22.863539875250098 50-51 21.52138700762201 26.684437835385 28.78489832143469 23.0092768355583 52-53 21.18224084005673 27.012883249271308 28.64049942549788 23.16437648517409 54-55 21.0745000133996 26.797894234340692 28.85743754096583 23.270168211293882 56-57 21.55611300859711 26.561330834187107 28.424294316170553 23.458261841045232 58-59 21.215658977677442 26.597746879773894 28.669836507641104 23.51675763490756 60-61 21.459547315781744 26.56953930614263 28.438463446458684 23.532449931616938 62-63 21.63079891542181 26.256708965049214 28.39245903876722 23.72003308076176 64-65 21.289938543469457 26.162799578987332 28.349238172917207 24.198023704626006 66-67 21.66156006838405 26.07579469909679 27.97500097983076 24.2876442526884 68-69 21.441240430136396 26.19915534503602 28.101044035209732 24.25856018961785 70-71 21.576352730456225 25.94453419743317 28.10193279140923 24.377180280701378 72-73 21.923596347628504 26.065588100338026 27.848481083929606 24.162334468103865 74-75 22.09082553035483 26.09440052427902 27.78172954229385 24.033044403072303 76-77 22.10429422428255 25.992011846135952 27.72102985860785 24.18266407097365 78-79 22.246586360164308 26.103970265234818 27.616650196459336 24.03279317814154 80-81 22.436915449047813 25.97955401970727 27.575517409758483 24.008013121486435 82-83 22.730105731496486 25.614233787202846 27.953653325694276 23.702007155606395 84-85 22.948994514143177 25.733086568470675 27.44964057553485 23.868278341851294 86-87 22.754023522671847 25.817687274460884 27.65863230776781 23.769656895099462 88-89 23.253866333529025 25.235364223436097 27.52429577284039 23.986473670194485 90-91 23.76566697040926 25.041110420824975 27.371815241367685 23.821407367398077 92-93 24.069828762252374 24.889495762126167 27.2946594376078 23.74601603801366 94-95 23.719269742132965 24.774797842366443 27.404778411343937 24.10115400415665 96-97 24.053800860887897 24.50450364721676 27.230293898383774 24.211401593511564 98-99 24.138877494238812 24.598645363930096 27.053503767246617 24.20897337458447 100 24.813705131711146 24.368440612531717 26.76495542847068 24.05289882728645 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2287.0 1 1591.5 2 1725.5 3 2548.0 4 1855.0 5 1261.0 6 1743.5 7 2784.5 8 4225.5 9 4969.5 10 4130.0 11 3268.0 12 3262.5 13 3550.0 14 3961.5 15 4388.5 16 4806.5 17 5228.5 18 5730.0 19 6285.0 20 6912.5 21 7576.5 22 8525.5 23 9907.5 24 11473.0 25 13456.0 26 16496.5 27 20063.5 28 24235.0 29 30327.0 30 37852.0 31 45029.0 32 53964.5 33 62815.5 34 70892.5 35 79077.5 36 87453.0 37 98736.5 38 105681.0 39 107081.5 40 107767.0 41 109218.5 42 113530.0 43 120493.0 44 129963.5 45 141863.5 46 152285.0 47 162080.5 48 170463.5 49 173920.5 50 173898.5 51 174491.5 52 172829.0 53 166771.5 54 158101.0 55 142711.0 56 127241.0 57 110596.5 58 92853.5 59 78637.5 60 62825.0 61 48249.0 62 37251.0 63 28174.0 64 20305.0 65 13872.0 66 9806.5 67 7225.0 68 5324.0 69 3962.5 70 3013.5 71 2266.5 72 1650.0 73 1232.0 74 913.5 75 673.5 76 483.5 77 329.5 78 231.5 79 168.5 80 135.5 81 98.0 82 68.0 83 61.5 84 55.5 85 39.5 86 34.5 87 23.0 88 13.5 89 14.5 90 17.0 91 16.5 92 8.5 93 5.5 94 4.0 95 2.5 96 4.0 97 4.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21710970699703128 2 0.14618353080669574 3 0.022331856393144823 4 0.021705963557014082 5 0.02576174913514128 6 0.05345124820556524 7 0.06003564084166063 8 0.053225926784558175 9 0.04060792720816244 10-11 0.042648337853948656 12-13 0.04033253436026492 14-15 0.020541802881810903 16-17 0.01865160651669607 18-19 0.01816341010451409 20-21 0.02115517786121903 22-23 0.02448492774943457 24-25 0.021105106434328568 26-27 0.01871419580030914 28-29 0.01868916008686391 30-31 0.01693666014569784 32-33 0.025223481296068842 34-35 0.0261998741204328 36-37 0.0245099634628798 38-39 0.02284508851877203 40-41 0.024222052758259657 42-43 0.029805016856545862 44-45 0.03935614153590096 46-47 0.043787462815706606 48-49 0.03969412366741156 50-51 0.028090070485547635 52-53 0.04272344499428435 54-55 0.04098346290984089 56-57 0.04332430211696986 58-59 0.04694196270980554 60-61 0.024547517033047642 62-63 0.025949516985980504 64-65 0.026663034819169543 66-67 0.03148240965737625 68-69 0.03557574880567129 70-71 0.03869269512960238 72-73 0.042811069991342654 74-75 0.025048231301952235 76-77 0.018914481507870978 78-79 0.018013195823842712 80-81 0.021130142147773798 82-83 0.019577927914169565 84-85 0.011979588883542374 86-87 0.013268928125971698 88-89 0.0187642672271996 90-91 0.019552892200724332 92-93 0.019577927914169565 94-95 0.02347098135490277 96-97 0.02440982060909888 98-99 0.02984257042671371 100 0.02909149902335682 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3994294.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.49013563499562 #Duplication Level Percentage of deduplicated Percentage of total 1 79.9323090484979 53.147100702530125 2 12.742059161804622 16.94442483875056 3 3.437026181575304 6.85585010981919 4 1.3900876514231721 3.6970846595063693 5 0.7166227262260506 2.382417113294522 6 0.42877742788261003 1.7105681602283576 7 0.2792844544631842 1.2998762880602013 8 0.2064891743115665 1.0983594565707449 9 0.15373034553215645 0.9199396373083042 >10 0.6772735701484066 7.638292963488534 >50 0.024733877000796548 1.1080185745404088 >100 0.010583983130669708 1.30475674685838 >500 5.111990059343617E-4 0.23053508011570173 >1k 3.8339924832117377E-4 0.5686758501137691 >5k 4.2599916480130315E-5 0.21405007716838914 >10k+ 8.519983296026063E-5 0.8800497416464008 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16959 0.4245806643176492 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14111 0.3532789524256352 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7557 0.18919488650560023 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.510714033568885E-5 2 0.0 0.0 0.0 2.503571344522962E-5 7.510714033568885E-5 3 0.0 0.0 0.0 2.503571344522962E-5 1.251785672261481E-4 4 0.0 0.0 0.0 2.503571344522962E-5 1.251785672261481E-4 5 0.0 0.0 0.0 5.007142689045924E-5 1.251785672261481E-4 6 0.0 0.0 0.0 5.007142689045924E-5 1.502142806713777E-4 7 0.0 0.0 0.0 5.007142689045924E-5 1.502142806713777E-4 8 0.0 0.0 0.0 7.510714033568885E-5 1.502142806713777E-4 9 0.0 0.0 0.0 7.510714033568885E-5 1.502142806713777E-4 10-11 0.0 0.0 0.0 1.6273213739399255E-4 1.502142806713777E-4 12-13 0.0 0.0 0.0 2.753928478975258E-4 3.004285613427554E-4 14-15 0.0 0.0 0.0 3.004285613427554E-4 3.5049998823321465E-4 16-17 0.0 0.0 0.0 3.880535584010591E-4 3.755357016784443E-4 18-19 0.0 0.0 0.0 5.25749982349822E-4 4.0057141512367394E-4 20-21 0.0 0.0 0.0 0.0010139463945317995 4.3812498529151837E-4 22-23 0.0 0.0 0.0 0.003530035595777376 4.506428420141332E-4 24-25 0.0 0.0 0.0 0.009112999694063581 4.63160698736748E-4 26-27 0.0 3.7553570167844434E-5 0.0 0.019089731501987582 5.88339265962896E-4 28-29 0.0 5.007142689045924E-5 0.0 0.051385801846333796 6.258928361307405E-4 30-31 0.0 5.007142689045924E-5 0.0 0.1539070484045491 6.634464062985849E-4 32-33 0.0 5.007142689045924E-5 0.0 0.37085402326418637 6.759642630211997E-4 34-35 0.0 5.007142689045924E-5 0.0 0.7056566191672421 6.759642630211997E-4 36-37 0.0 5.007142689045924E-5 0.0 1.1964942991176915 6.759642630211997E-4 38-39 0.0 5.007142689045924E-5 0.0 1.8546706877360555 6.759642630211997E-4 40-41 0.0 5.007142689045924E-5 0.0 2.5210963439346226 6.759642630211997E-4 42-43 0.0 5.007142689045924E-5 0.0 3.162023125989224 6.759642630211997E-4 44-45 0.0 5.007142689045924E-5 0.0 3.9342246714938858 6.884821197438145E-4 46-47 0.0 5.007142689045924E-5 0.0 4.809773642100456 7.635892600795034E-4 48-49 0.0 5.007142689045924E-5 0.0 5.6837078091898094 8.762499705830367E-4 50-51 0.0 5.007142689045924E-5 0.0 6.5360236377192065 9.012856840282664E-4 52-53 0.0 5.007142689045924E-5 0.0 7.33398693235901 0.0011015713915901032 54-55 0.0 5.007142689045924E-5 0.0 8.19862033190346 0.0011015713915901032 56-57 0.0 5.007142689045924E-5 0.0 9.163221335234713 0.0012267499588162513 58-59 0.0 7.510714033568885E-5 0.0 10.197546800510928 0.0012768213857067105 60-61 0.0 7.510714033568885E-5 0.0 11.204332980997393 0.0013018570991519401 62-63 0.0 7.510714033568885E-5 0.0 12.195859894138989 0.001376964239487629 64-65 0.0 7.510714033568885E-5 0.0 13.172490557780673 0.0013894820962102438 66-67 0.0 7.510714033568885E-5 0.0 14.226306325974 0.0014019999529328586 68-69 0.0 7.510714033568885E-5 0.0 15.355629805918142 0.0014019999529328586 70-71 0.0 7.510714033568885E-5 0.0 16.508511892214244 0.0014270356663780882 72-73 0.0 7.510714033568885E-5 0.0 17.574507534998677 0.0014771070932685477 74-75 0.0 7.510714033568885E-5 0.0 18.599507197016543 0.0015021428067137773 76-77 0.0 7.510714033568885E-5 0.0 19.683303232060535 0.0015522142336042365 78-79 0.0 7.510714033568885E-5 0.0 20.80441499799464 0.0015772499470494662 80-81 0.0 7.510714033568885E-5 0.0 21.9357413350144 0.0016022856604946958 82-83 0.0 1.0014285378091849E-4 0.0 23.044660708500675 0.0017024285142756143 84-85 0.0 1.251785672261481E-4 0.0 24.097813030287703 0.0017024285142756143 86-87 0.0 1.251785672261481E-4 0.0 25.148862352145336 0.0017650177978886883 88 0.0 1.502142806713777E-4 0.0 25.950393235951083 0.0018276070815017623 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6800 0.0 57.716656 1 GTATCAA 12785 0.0 43.264576 1 GTGGTAT 2325 0.0 36.27397 1 ATCAACG 15665 0.0 34.781963 3 TACCTGG 2970 0.0 34.712196 2 CAACGCA 15780 0.0 34.558273 5 TATCAAC 16155 0.0 34.09364 2 TCAACGC 16725 0.0 32.77348 4 AACGCAG 17020 0.0 32.482452 6 ACGCAGA 18245 0.0 29.709267 7 CGCAGAG 18570 0.0 29.0855 8 GTACTAG 1490 0.0 28.77532 1 GTACATG 13220 0.0 28.68993 1 TACATGG 13495 0.0 27.627829 2 ACCTGGG 3670 0.0 27.540604 3 TGGTATC 3190 0.0 26.858093 2 ACATGGG 13470 0.0 26.419788 3 GCAGAGT 19715 0.0 26.274649 9 CATGGGG 10300 0.0 24.326529 4 GAGTACT 13305 0.0 22.802748 12-13 >>END_MODULE