FastQCFastQC Report
Mon 6 Jun 2016
ERR1211461_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211461_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3994294
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154260.3862009156061121No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134710.3372560958206882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73560.18416270810310909No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73950.056.3294531
GTATCAA127650.038.6362231
GTGGTAT26350.035.68531
TACCTGG26700.035.1981122
ATCAACG155350.031.3976863
CAACGCA156050.031.2855
TATCAAC159200.030.8737432
TCAACGC166250.029.3963974
AACGCAG169900.028.982516
TGGTATC33850.026.5140062
GTACATG135650.026.4797291
ACCTGGG34900.026.3901713
ACGCAGA185300.026.2188227
TACATGG136700.025.7118762
CGCAGAG188150.025.6468628
GTACTAG13400.025.6128581
ACATGGG132500.025.5345483
CATGGGG101350.023.7857064
GCAGAGT202050.023.1615989
GAGTACT133400.022.20626812-13
Produced by FastQC (version 0.11.5)