##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211461_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3994294 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00528704196536 33.0 31.0 34.0 30.0 34.0 2 32.13997617601508 33.0 31.0 34.0 30.0 34.0 3 32.21443789565816 34.0 31.0 34.0 30.0 34.0 4 35.875143642405895 37.0 35.0 37.0 35.0 37.0 5 35.727664012714136 37.0 35.0 37.0 35.0 37.0 6 35.76696357353765 37.0 35.0 37.0 33.0 37.0 7 35.696717116967356 37.0 35.0 37.0 33.0 37.0 8 35.68634056481571 37.0 35.0 37.0 33.0 37.0 9 37.260565947323855 39.0 37.0 39.0 34.0 39.0 10-11 37.23999410158591 39.0 37.0 39.0 33.5 39.0 12-13 37.15419283107353 39.0 37.0 39.0 33.0 39.0 14-15 38.33594697836464 40.0 38.0 41.0 33.0 41.0 16-17 38.322171327398536 40.0 38.0 41.0 33.0 41.0 18-19 38.331551708512194 40.0 38.0 41.0 33.5 41.0 20-21 38.29231573840083 40.0 38.0 41.0 34.0 41.0 22-23 38.18362857115676 40.0 37.5 41.0 33.0 41.0 24-25 38.06111568151969 40.0 37.0 41.0 33.0 41.0 26-27 37.92510566322859 40.0 37.0 41.0 33.0 41.0 28-29 37.83892384987184 40.0 37.0 41.0 33.0 41.0 30-31 37.87937355137103 40.0 37.0 41.0 33.0 41.0 32-33 37.70968398921061 40.0 37.0 41.0 33.0 41.0 34-35 37.585828309082906 40.0 36.0 41.0 32.5 41.0 36-37 37.43321623295631 40.0 36.0 41.0 32.0 41.0 38-39 37.30921421908353 40.0 36.0 41.0 31.5 41.0 40-41 37.23195350667727 39.0 36.0 41.0 31.0 41.0 42-43 37.08319279952853 39.0 36.0 41.0 31.0 41.0 44-45 36.90949351750272 39.0 35.5 41.0 31.0 41.0 46-47 36.67006184321935 39.0 35.0 41.0 30.0 41.0 48-49 36.42718300155171 39.0 35.0 41.0 30.0 41.0 50-51 36.22962944139815 39.0 35.0 40.0 29.0 41.0 52-53 36.01236839351334 38.5 35.0 40.0 28.5 41.0 54-55 35.8032662342832 38.0 35.0 40.0 28.0 41.0 56-57 35.55749739503402 38.0 34.0 40.0 28.0 41.0 58-59 35.27833517012017 38.0 34.0 40.0 27.0 41.0 60-61 35.01326204330478 37.0 34.0 40.0 26.5 41.0 62-63 34.71274861089344 37.0 34.0 40.0 26.0 41.0 64-65 34.35376351865936 36.0 33.0 39.0 26.0 41.0 66-67 33.99078422870224 36.0 33.0 39.0 26.0 40.5 68-69 33.61092348234757 35.0 32.5 38.5 25.0 40.0 70-71 33.232725608079924 35.0 32.0 38.0 24.5 40.0 72-73 32.86823841710199 35.0 32.0 37.0 24.0 39.5 74-75 32.49889254521575 35.0 31.0 37.0 23.5 39.0 76-77 31.47315332822271 34.0 30.5 35.5 22.5 38.0 78-79 31.958058169979473 34.0 31.0 36.0 23.0 38.0 80-81 31.84335692365159 34.5 31.0 35.0 23.0 37.0 82-83 31.572780821842358 34.0 31.0 35.0 23.0 37.0 84-85 31.309291954973773 34.0 31.0 35.0 21.5 36.0 86-87 31.060749784567687 34.0 31.0 35.0 20.0 36.0 88-89 30.827089843661984 34.0 30.0 35.0 20.0 35.5 90-91 30.60669219641819 34.0 30.0 35.0 19.5 35.0 92-93 30.42332812757398 34.0 30.0 35.0 19.0 35.0 94-95 30.25936673664983 34.0 29.0 35.0 19.0 35.0 96-97 30.08770448544849 34.0 29.0 35.0 18.0 35.0 98-99 29.879325107265515 34.0 29.0 35.0 18.0 35.0 100 27.991332886362397 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.23340535126709128 1101 2 -0.19779958635136197 1101 3 -0.23568971007495065 1101 4 -0.12002505380797857 1101 5 -0.11244370002091841 1101 6 -0.23386235155832225 1101 7 -0.22382833150935966 1101 8 -0.26263799508149077 1101 9 -0.38081613284798266 1101 10-11 -0.3884340570455862 1101 12-13 -0.3670336550836666 1101 14-15 -0.5146445474764292 1101 16-17 -0.6014021989253351 1101 18-19 -0.621655835239018 1101 20-21 -0.5978130910498081 1101 22-23 -0.5280069344805511 1101 24-25 -0.6801427446583403 1101 26-27 -0.5900028234958228 1101 28-29 -0.6310644153086571 1101 30-31 -0.5628487499928525 1101 32-33 -0.5393688817642683 1101 34-35 -0.5532074696540121 1101 36-37 -0.6109318005027831 1101 38-39 -0.6591518089047312 1101 40-41 -0.6027069027894356 1101 42-43 -0.5781520853581696 1101 44-45 -0.6907297527696841 1101 46-47 -0.6885716300794726 1101 48-49 -0.6580765324487743 1101 50-51 -0.7124535185108556 1101 52-53 -0.7440062809250705 1101 54-55 -0.7346526325973386 1101 56-57 -0.7760559510043166 1101 58-59 -0.7887366147548391 1101 60-61 -0.7084435275866952 1101 62-63 -0.817384586221074 1101 64-65 -0.7271180508529937 1101 66-67 -0.8288806701188918 1101 68-69 -0.8154088021333834 1101 70-71 -0.828031448554583 1101 72-73 -0.9200238922136705 1101 74-75 -0.9229373077993017 1101 76-77 -0.6725730149984486 1101 78-79 -0.6758175192634432 1101 80-81 -0.6005439247460913 1101 82-83 -0.5961856976392959 1101 84-85 -0.6465209776541236 1101 86-87 -0.6613468778036697 1101 88-89 -0.7732383496737647 1101 90-91 -0.9572673361680728 1101 92-93 -1.0420125230938702 1101 94-95 -1.0999551195864257 1101 96-97 -1.202394973065271 1101 98-99 -1.1126050488313197 1101 100 -1.0686070461045887 1102 1 -0.3387376618542497 1102 2 -0.25164075261533725 1102 3 -0.26620774191768604 1102 4 -0.1289431926270197 1102 5 -0.2574594829614867 1102 6 -0.25455984418151445 1102 7 -0.2315454560288117 1102 8 -0.2837851061000407 1102 9 -0.4867723690134653 1102 10-11 -0.5139044028721145 1102 12-13 -0.5057470257872012 1102 14-15 -0.6074037468485827 1102 16-17 -0.7421971862859422 1102 18-19 -0.7512002931772415 1102 20-21 -0.6883009729196914 1102 22-23 -0.7377079664114845 1102 24-25 -0.7016852683192525 1102 26-27 -0.8188307701147721 1102 28-29 -0.8021984590755693 1102 30-31 -0.6901221990736701 1102 32-33 -0.6650335195282437 1102 34-35 -0.6421127364392945 1102 36-37 -0.7446774936531639 1102 38-39 -0.71930877487641 1102 40-41 -0.6986404373540722 1102 42-43 -0.6628973210196989 1102 44-45 -0.6596759134552883 1102 46-47 -0.7555252258653553 1102 48-49 -0.7961126205943501 1102 50-51 -0.9203273728886643 1102 52-53 -0.8373970834163558 1102 54-55 -0.9697687166286784 1102 56-57 -0.9059608675548532 1102 58-59 -0.9641772187913489 1102 60-61 -0.963849770191274 1102 62-63 -0.9154481561047163 1102 64-65 -0.954589889131725 1102 66-67 -0.9700133535336022 1102 68-69 -1.005581758223478 1102 70-71 -0.9644694507921088 1102 72-73 -0.9311129617952645 1102 74-75 -0.9028409781962239 1102 76-77 -0.7207581099364475 1102 78-79 -0.7906111064566588 1102 80-81 -0.7027726569742221 1102 82-83 -0.7881511473167215 1102 84-85 -0.845659565290628 1102 86-87 -0.9668847612977984 1102 88-89 -0.9698720913931105 1102 90-91 -0.8652027034658687 1102 92-93 -0.9629231272746601 1102 94-95 -0.9901695266808126 1102 96-97 -1.0099009690703227 1102 98-99 -0.9964964416444779 1102 100 -0.9685033337165336 1103 1 -0.35046755418342457 1103 2 -0.31486257377646965 1103 3 -0.32538667787007824 1103 4 -0.1614180903511766 1103 5 -0.1747202032770261 1103 6 -0.29152327588224836 1103 7 -0.3039266775295104 1103 8 -0.32812415909246795 1103 9 -0.4229540604739128 1103 10-11 -0.39685356213037437 1103 12-13 -0.51599998632053 1103 14-15 -0.6482903637303465 1103 16-17 -0.7835856908209635 1103 18-19 -0.7058077773297171 1103 20-21 -0.68173842778058 1103 22-23 -0.7565927868061877 1103 24-25 -0.7382821408847065 1103 26-27 -0.7944561267877006 1103 28-29 -0.7826315892648452 1103 30-31 -0.6783269512397538 1103 32-33 -0.7522627934270076 1103 34-35 -0.7144881959208575 1103 36-37 -0.4866088119636771 1103 38-39 -0.7455434058743293 1103 40-41 -0.7579314958843497 1103 42-43 -0.7648144947572035 1103 44-45 -0.7469539277214139 1103 46-47 -0.7458792901016125 1103 48-49 -0.6832289036657713 1103 50-51 -0.6378308083939075 1103 52-53 -0.921137967783686 1103 54-55 -0.7948684666517636 1103 56-57 -0.9303377999032207 1103 58-59 -0.9896658261744662 1103 60-61 -0.8847877637614943 1103 62-63 -0.9841623654806995 1103 64-65 -0.8240355197452587 1103 66-67 -0.9087421337141564 1103 68-69 -0.9044699647910761 1103 70-71 -0.8178943791812543 1103 72-73 -1.1482068992828829 1103 74-75 -1.1472202863908407 1103 76-77 -1.0176263341374039 1103 78-79 -0.8226309230665869 1103 80-81 -0.8855530995762244 1103 82-83 -0.846019381778806 1103 84-85 -0.897440108135978 1103 86-87 -0.8312749305038345 1103 88-89 -0.7789001887349265 1103 90-91 -0.9455429309630148 1103 92-93 -0.8976653795295455 1103 94-95 -0.9517235984945138 1103 96-97 -1.065262093753315 1103 98-99 -1.1278082797522657 1103 100 -1.0919517522001456 1104 1 -0.3401002707953573 1104 2 -0.2425669985388268 1104 3 -0.24033473178403142 1104 4 -0.13241215586225508 1104 5 -0.08940273437825397 1104 6 -0.2618792525397211 1104 7 -0.18868761431993875 1104 8 -0.24464295267048897 1104 9 -0.33281145974051896 1104 10-11 -0.3962120750790561 1104 12-13 -0.40699213648380095 1104 14-15 -0.5303698908247938 1104 16-17 -0.5973022069886653 1104 18-19 -0.6527751175603598 1104 20-21 -0.5903978851193017 1104 22-23 -0.6622989786313624 1104 24-25 -0.7585292979833866 1104 26-27 -0.7857681709271986 1104 28-29 -0.7381960607987565 1104 30-31 -0.8603453880070191 1104 32-33 -0.710677348834615 1104 34-35 -0.7149860318867596 1104 36-37 -0.710010346095487 1104 38-39 -0.9276472609751778 1104 40-41 -0.7988081845569184 1104 42-43 -0.5700898877896634 1104 44-45 -0.6979144591995379 1104 46-47 -0.664649775827634 1104 48-49 -0.5433811738163143 1104 50-51 -0.6976401695018453 1104 52-53 -0.6150812209710423 1104 54-55 -0.5162353298990183 1104 56-57 -0.6591723571026122 1104 58-59 -0.7001645580288596 1104 60-61 -0.787304669795148 1104 62-63 -0.8666415669660239 1104 64-65 -0.6842522784207006 1104 66-67 -0.7415103364691618 1104 68-69 -0.825300839579846 1104 70-71 -0.7028637645090754 1104 72-73 -0.7131422099553859 1104 74-75 -0.584397187296311 1104 76-77 -0.5490802839243969 1104 78-79 -0.5934507537127693 1104 80-81 -0.7158750633637929 1104 82-83 -0.6938739605413069 1104 84-85 -0.7442162997448989 1104 86-87 -0.6555250100525534 1104 88-89 -0.7346186604109484 1104 90-91 -0.5935496563052283 1104 92-93 -0.8497174275615258 1104 94-95 -0.9035658078173192 1104 96-97 -0.9521728539919287 1104 98-99 -1.0010528789348427 1104 100 -1.1129774827354204 1105 1 -0.4359102032371389 1105 2 -0.39984918242036827 1105 3 -0.42975423098139487 1105 4 -0.23387363607309908 1105 5 -0.2569937579574315 1105 6 -0.31689226281553573 1105 7 -0.3383809169661802 1105 8 -0.3551043599821284 1105 9 -0.5091932343451191 1105 10-11 -0.517134220219873 1105 12-13 -0.5765650513062823 1105 14-15 -0.6754867973397509 1105 16-17 -0.697335309176971 1105 18-19 -0.7068661389079267 1105 20-21 -0.7742102827442707 1105 22-23 -0.6555361643651381 1105 24-25 -0.6572719727294611 1105 26-27 -0.7607676874120912 1105 28-29 -0.841199545083029 1105 30-31 -0.7735103142672415 1105 32-33 -0.7107621499462553 1105 34-35 -0.79157564360181 1105 36-37 -0.9411473802286778 1105 38-39 -0.8521100013007867 1105 40-41 -0.8270465456043468 1105 42-43 -0.7718004154757381 1105 44-45 -0.7952370128734358 1105 46-47 -0.9853345150493382 1105 48-49 -0.9162779202803293 1105 50-51 -0.9271726029515648 1105 52-53 -0.8964346839062571 1105 54-55 -0.9998528337411017 1105 56-57 -1.0158258597604615 1105 58-59 -0.9453477805008603 1105 60-61 -0.9589673741174778 1105 62-63 -0.8813271854413642 1105 64-65 -0.9263688620963393 1105 66-67 -0.9752361105024647 1105 68-69 -1.0019688923231627 1105 70-71 -0.9674646190662486 1105 72-73 -1.0327594965876052 1105 74-75 -0.8702371869392493 1105 76-77 -0.9130844808355576 1105 78-79 -0.8175180530651538 1105 80-81 -0.7858404436819022 1105 82-83 -0.7838322815388032 1105 84-85 -0.6739890030109308 1105 86-87 -0.7559633350966983 1105 88-89 -0.9684659455107969 1105 90-91 -0.8370103376003328 1105 92-93 -1.031334108684053 1105 94-95 -0.9201474378137 1105 96-97 -0.9636189956157359 1105 98-99 -0.947226712287268 1105 100 -0.9849495995348185 1106 1 -0.3475421634969962 1106 2 -0.3694292994705748 1106 3 -0.35470227106543817 1106 4 -0.25619549157897836 1106 5 -0.2806526506670153 1106 6 -0.3023741446187529 1106 7 -0.3254371266014431 1106 8 -0.33677627477408834 1106 9 -0.4894404541557762 1106 10-11 -0.5032595964513717 1106 12-13 -0.5979219029725726 1106 14-15 -0.7312672533870241 1106 16-17 -0.7056958343480417 1106 18-19 -0.7756535311146493 1106 20-21 -0.7650185452581013 1106 22-23 -0.7352790958592692 1106 24-25 -0.8013999443043431 1106 26-27 -0.8336570684918385 1106 28-29 -0.7604116714170601 1106 30-31 -0.8740026026876748 1106 32-33 -0.8092776384097107 1106 34-35 -0.8215153477824231 1106 36-37 -0.8585174493414911 1106 38-39 -0.8681574477192413 1106 40-41 -0.864365600524728 1106 42-43 -0.808633364848987 1106 44-45 -0.8243186147278649 1106 46-47 -0.7746539124057819 1106 48-49 -0.802015202311182 1106 50-51 -0.8099206303771851 1106 52-53 -0.8758940446849621 1106 54-55 -0.8934189138396817 1106 56-57 -1.0231001016542223 1106 58-59 -1.1102041457689182 1106 60-61 -1.1170827611671754 1106 62-63 -0.9841717095924309 1106 64-65 -1.0627457056626923 1106 66-67 -1.0264479719607422 1106 68-69 -1.0148696952552356 1106 70-71 -0.9284389813614027 1106 72-73 -0.9946083147258875 1106 74-75 -1.0560321635925014 1106 76-77 -0.9912365114704116 1106 78-79 -0.8962481274754879 1106 80-81 -0.8128349050713233 1106 82-83 -0.8610294058076065 1106 84-85 -0.9137314149973612 1106 86-87 -0.852674009918907 1106 88-89 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0.824035634620742 2311 30-31 0.9412466568821145 2311 32-33 1.056040415258444 2311 34-35 0.9911536456478771 2311 36-37 1.0729370944175756 2311 38-39 1.0681343285531426 2311 40-41 1.0271559825109762 2311 42-43 1.0651263703848741 2311 44-45 1.0616619703314072 2311 46-47 1.0597201630278192 2311 48-49 1.1250699766227044 2311 50-51 1.1589154311246546 2311 52-53 1.1191861246708754 2311 54-55 1.0916627560159 2311 56-57 1.1712799757783472 2311 58-59 1.0975482384290984 2311 60-61 1.1727855876416555 2311 62-63 1.1880227585651824 2311 64-65 1.2575313301071276 2311 66-67 1.2149030034064765 2311 68-69 1.188785646453205 2311 70-71 1.1957885791735947 2311 72-73 1.2222952246167367 2311 74-75 1.1901427347705393 2311 76-77 1.1206904269082116 2311 78-79 0.9925741384712801 2311 80-81 1.0816801890800924 2311 82-83 1.095443526844253 2311 84-85 1.060839070483528 2311 86-87 1.1643315973158188 2311 88-89 1.2051195189042438 2311 90-91 1.2135608819069539 2311 92-93 1.2063255517325224 2311 94-95 1.249233126491081 2311 96-97 1.2918922986740817 2311 98-99 1.2910500980839572 2311 100 1.4647323375081207 2312 1 0.165266666479976 2312 2 0.12520997145188062 2312 3 0.32165080882746366 2312 4 0.2649871736744771 2312 5 0.4139273678068065 2312 6 0.444517613246461 2312 7 0.495942621149986 2312 8 0.3544398051145592 2312 9 0.3856533409380205 2312 10-11 0.34886042381023685 2312 12-13 0.4853791224523505 2312 14-15 0.8400434942069026 2312 16-17 0.9433492732811359 2312 18-19 0.9295866498777414 2312 20-21 0.8740653403744645 2312 22-23 0.5450026086702238 2312 24-25 0.5264965512027686 2312 26-27 0.8315511876898682 2312 28-29 0.9786796207715796 2312 30-31 0.9550813255113013 2312 32-33 0.7838208134498004 2312 34-35 0.9237356450791907 2312 36-37 1.0740398982601036 2312 38-39 1.09354018601379 2312 40-41 1.072601201928805 2312 42-43 0.9837317528258609 2312 44-45 0.8453185065906794 2312 46-47 1.0028785976931047 2312 48-49 1.0789165764967024 2312 50-51 1.0950117954401932 2312 52-53 1.157870231026216 2312 54-55 1.13140249876934 2312 56-57 1.2080789454540337 2312 58-59 1.1964500063719612 2312 60-61 1.1188335021827172 2312 62-63 1.1568286862752188 2312 64-65 1.1731476569563313 2312 66-67 1.1922070996146843 2312 68-69 1.1585617962293568 2312 70-71 0.957952706269225 2312 72-73 1.1012143876454914 2312 74-75 1.153733593429891 2312 76-77 1.0571051435969068 2312 78-79 1.002205775226205 2312 80-81 0.8285508019780998 2312 82-83 0.9442558104510574 2312 84-85 0.7261980950206386 2312 86-87 0.9412319268188902 2312 88-89 0.6090262767836059 2312 90-91 0.9463128179465627 2312 92-93 1.1155925607255632 2312 94-95 1.19598688009415 2312 96-97 1.1754296339922412 2312 98-99 1.3429008348661995 2312 100 1.449572297142602 2313 1 -0.3095385007945275 2313 2 0.039566117108108756 2313 3 0.28496484708017533 2313 4 0.06881036843798682 2313 5 0.26080359808450737 2313 6 0.35628397837341197 2313 7 0.14806408498036205 2313 8 0.16646734603008184 2313 9 0.3563126616300494 2313 10-11 0.49356757986244304 2313 12-13 0.6449847630187477 2313 14-15 0.7251927931378859 2313 16-17 0.8326851937127273 2313 18-19 0.6872465589399397 2313 20-21 0.8958765254644661 2313 22-23 0.9666725200253339 2313 24-25 0.8370137112783951 2313 26-27 1.0706866512693267 2313 28-29 0.9094266129453885 2313 30-31 0.9257906576678891 2313 32-33 1.0269156189199151 2313 34-35 0.9691877599671201 2313 36-37 0.8442271600458895 2313 38-39 0.6400397512255438 2313 40-41 0.8103263292287437 2313 42-43 0.9325238202115571 2313 44-45 1.1304504139915963 2313 46-47 1.1178122546300315 2313 48-49 1.0883569542649596 2313 50-51 1.1045753632130868 2313 52-53 1.082682514984043 2313 54-55 1.1259204029601761 2313 56-57 1.261742523177297 2313 58-59 1.1948428472913761 2313 60-61 1.214877469874267 2313 62-63 1.2048422880091891 2313 64-65 1.3018004406827117 2313 66-67 1.3693491131385471 2313 68-69 1.3595463861223678 2313 70-71 1.2576738144817483 2313 72-73 1.3353289770626091 2313 74-75 0.9690100625166664 2313 76-77 1.1196146721653122 2313 78-79 0.9101907155455642 2313 80-81 1.025770990862231 2313 82-83 1.011763111000068 2313 84-85 1.052128476985363 2313 86-87 1.2236524643988709 2313 88-89 1.176465222562701 2313 90-91 1.3332953874802342 2313 92-93 1.0282579641268583 2313 94-95 1.0700325090661913 2313 96-97 1.390048015368592 2313 98-99 1.471689657563445 2313 100 1.5383605574271826 2314 1 -0.43837545555648916 2314 2 -0.15918565664591 2314 3 0.0646853456224008 2314 4 -0.19389229528817964 2314 5 0.1395944658532713 2314 6 0.21121130717808967 2314 7 0.1943952882216209 2314 8 0.2078532958574968 2314 9 0.2066846908046429 2314 10-11 0.40573708000053443 2314 12-13 0.5689226874102218 2314 14-15 0.6408688162011131 2314 16-17 0.7874942609119842 2314 18-19 0.8129206875046222 2314 20-21 0.5193205367403024 2314 22-23 0.6614174463745783 2314 24-25 0.8652067916309818 2314 26-27 0.8664420221896876 2314 28-29 0.7737533074841991 2314 30-31 0.8164002032195299 2314 32-33 0.8382208433822811 2314 34-35 0.8594424696836853 2314 36-37 0.8262931974352057 2314 38-39 0.807951418615545 2314 40-41 0.8411804657659729 2314 42-43 0.6362853011448095 2314 44-45 0.6461205868977515 2314 46-47 0.8146839001431658 2314 48-49 0.6828664968234932 2314 50-51 0.8997641702300072 2314 52-53 0.9578114225148298 2314 54-55 0.9882566835935762 2314 56-57 0.8794784647736833 2314 58-59 0.9339190394649535 2314 60-61 0.8983542964497673 2314 62-63 0.5892987097810192 2314 64-65 0.7886608008347054 2314 66-67 0.8608992925206849 2314 68-69 1.0035463017712658 2314 70-71 1.0796050669731727 2314 72-73 1.0147061870274712 2314 74-75 1.034127667680039 2314 76-77 0.8253259220173561 2314 78-79 0.8602779917260399 2314 80-81 0.7969754293627922 2314 82-83 0.7893734714921798 2314 84-85 0.7277978097817517 2314 86-87 0.9207968054603057 2314 88-89 1.0850805822181115 2314 90-91 1.1242520990389195 2314 92-93 1.1391660581592937 2314 94-95 1.2604808011604511 2314 96-97 1.3042833522219972 2314 98-99 1.254520688020996 2314 100 1.1071903763580195 2315 1 -0.03498478766160673 2315 2 0.13107939420352466 2315 3 0.15228758501461215 2315 4 -0.0411728092501491 2315 5 0.29107522314237855 2315 6 0.44625599754312617 2315 7 0.4799948683524491 2315 8 0.4140460204637293 2315 9 0.5960751519355441 2315 10-11 0.5770477637956688 2315 12-13 0.6448483272570513 2315 14-15 0.6677603819846141 2315 16-17 0.7433268350426019 2315 18-19 0.8638236693245744 2315 20-21 0.7724396835572378 2315 22-23 0.9058861304010932 2315 24-25 0.8411231937150845 2315 26-27 0.9077336410302337 2315 28-29 0.9685293210409043 2315 30-31 0.9188329518009368 2315 32-33 0.9849545880398907 2315 34-35 0.9749708958522447 2315 36-37 1.0295190865793487 2315 38-39 0.9745602034538337 2315 40-41 0.941133395760005 2315 42-43 0.9930489203307502 2315 44-45 0.9377201381895546 2315 46-47 0.9246538999711476 2315 48-49 1.0506939917446232 2315 50-51 0.9858523820521228 2315 52-53 1.0567181368800007 2315 54-55 0.9044053721938639 2315 56-57 1.0382318837156248 2315 58-59 1.0073552477171006 2315 60-61 1.0700452421436921 2315 62-63 1.1216908552119875 2315 64-65 1.1081295143748378 2315 66-67 1.1503253765896417 2315 68-69 1.01511694742468 2315 70-71 0.6647689754937716 2315 72-73 0.9822192817985709 2315 74-75 1.106666556510838 2315 76-77 0.9622960134218772 2315 78-79 0.9106991847980588 2315 80-81 0.9566531726874068 2315 82-83 0.9721519038164423 2315 84-85 0.797506194988788 2315 86-87 0.8116804448976502 2315 88-89 0.8986085415655474 2315 90-91 0.9329046404313495 2315 92-93 0.9807641885811549 2315 94-95 1.0253596283600679 2315 96-97 1.1574891468078832 2315 98-99 1.0542062963034269 2315 100 0.9924301264468127 2316 1 0.5121449733582857 2316 2 0.5177473535544834 2316 3 0.498361183798707 2316 4 0.2857831249052154 2316 5 0.419660057017758 2316 6 0.428000474990192 2316 7 0.4912681329972415 2316 8 0.5019898396211175 2316 9 0.5003531338986846 2316 10-11 0.5321776540839878 2316 12-13 0.6234743605475828 2316 14-15 0.8142141866384733 2316 16-17 0.8383067151951025 2316 18-19 0.8532535465881139 2316 20-21 0.9298831457574792 2316 22-23 0.9197576121208826 2316 24-25 0.9882402825117182 2316 26-27 1.0078198787411665 2316 28-29 0.9983069499641246 2316 30-31 0.8919849371975204 2316 32-33 0.8526314874778649 2316 34-35 0.7896707727534462 2316 36-37 0.8058779474894564 2316 38-39 0.7848169285948785 2316 40-41 0.8607953588186135 2316 42-43 0.9406675706312484 2316 44-45 0.8815733950194797 2316 46-47 0.9596027752868466 2316 48-49 0.7173614810285684 2316 50-51 0.7462701345246288 2316 52-53 0.9362737276905833 2316 54-55 0.9856606078100612 2316 56-57 1.021605515502337 2316 58-59 0.9751939677246213 2316 60-61 0.9718401734502606 2316 62-63 1.0531295834456813 2316 64-65 1.0272079284079112 2316 66-67 1.0734769408845253 2316 68-69 1.0686147062822684 2316 70-71 1.0093444707044696 2316 72-73 1.039889335885654 2316 74-75 1.0721646953392536 2316 76-77 0.8546942855338742 2316 78-79 0.8831627570527552 2316 80-81 0.894150065838236 2316 82-83 0.9305222010652763 2316 84-85 0.8848178327708105 2316 86-87 0.8723292353075003 2316 88-89 0.830263911934285 2316 90-91 0.9444908717767859 2316 92-93 1.064748300316083 2316 94-95 0.9353979682008919 2316 96-97 1.072549486764263 2316 98-99 1.2002507313465287 2316 100 0.9343111975336775 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 6.0 12 24.0 13 143.0 14 600.0 15 1791.0 16 4171.0 17 7924.0 18 12451.0 19 17438.0 20 23228.0 21 30228.0 22 35592.0 23 36635.0 24 37192.0 25 40629.0 26 47671.0 27 57502.0 28 69232.0 29 85044.0 30 103740.0 31 127580.0 32 159449.0 33 204605.0 34 282382.0 35 386033.0 36 577332.0 37 848317.0 38 698645.0 39 98697.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.96445418329887 19.929007558633515 13.111634123663412 23.99490413440421 2 16.118467994594287 21.261779929068815 39.07746900954211 23.542283066794784 3 18.719102800144405 26.43806389815071 30.65412811375427 24.188705187950614 4 12.799313315553587 17.472343197995436 38.241467838083295 31.486875648367686 5 12.838311335684613 37.79375229555106 34.82279910639064 14.545137262373691 6 29.761730107999064 37.46829852784998 18.349525598265927 14.420445765885034 7 26.12264773294845 33.310742723666436 22.802028398073748 17.764581145311364 8 25.739044703448283 34.78075386840876 20.766831953108923 18.713369475034035 9 26.72141588879108 15.50676328647553 20.700027940429802 37.07179288430359 10-11 24.720818333695103 25.88369869485309 28.719741288943666 20.675741682508136 12-13 25.30996145244445 24.20906409579482 28.264631459882576 22.21634299187815 14-15 22.83035105716469 25.627304460051437 26.22757346889343 25.314771013890446 16-17 21.911708246631473 28.115812278598096 27.195035715345263 22.77744375942517 18-19 21.53415597349619 27.495935451922165 29.324055765549556 21.645852809032085 20-21 22.427445554514208 26.923409609233772 29.069245912950564 21.579898923301457 22-23 22.474985566911197 26.64323156983437 28.84710038870449 22.034682474549946 24-25 22.11415690189901 26.542526184865807 29.208893591154578 22.13442332208061 26-27 22.059993580843074 26.801907921650237 28.92896717167039 22.209131325836307 28-29 21.711742127082044 27.443423934902366 28.89448610645337 21.950347831562222 30-31 22.46589472366527 26.88536020529511 28.959166301558493 21.68957876948113 32-33 22.38417804370666 27.04854326146376 28.53653089002823 22.030747804801344 34-35 22.391348960333417 26.980721697102677 28.965459472542104 21.6624698700218 36-37 22.192161316121734 26.67341096159546 28.970678595528753 22.163749126754052 38-39 22.05849523336605 26.715161240200015 29.128646467772807 22.09769705866113 40-41 21.987174191157877 26.277035468130755 29.52220544827742 22.21358489243395 42-43 21.712284113691943 26.539081856036407 29.020810508753627 22.72782352151803 44-45 21.380403389665002 26.87750459763064 29.252836509631553 22.48925550307281 46-47 21.416872719078054 26.49540216214186 28.954159337460638 23.133565781319447 48-49 21.579160974886733 26.511918687332003 28.939571323844586 22.969349013936682 50-51 21.39054625249429 26.639757138208957 28.74663250103001 23.22306410826674 52-53 21.07216648181124 26.922887680891332 28.579312493059316 23.425633344238115 54-55 20.890602389366695 26.736637477595544 28.744485188487907 23.62827494454986 56-57 21.30787856953027 26.51431630172022 28.266857912049577 23.910947216699938 58-59 20.84360874375197 26.641525373122743 28.46151082365487 24.05335505947042 60-61 21.187115061866123 26.76036767541775 28.06789144189136 23.984625820824768 62-63 21.146317544891396 26.489534583580248 27.93670202498541 24.427445846542945 64-65 20.718491995902358 26.309415985959244 27.9352510581525 25.036840959985895 66-67 21.0378789823714 26.318507057263997 27.583172772567682 25.060441187796922 68-69 20.940090921513093 26.360362759227574 27.536916746960173 25.16262957229916 70-71 20.981308118601085 26.137098844672657 27.43487272613419 25.446720310592063 72-73 21.220248639156893 26.258076239272953 27.10583325099461 25.415841870575544 74-75 21.39272434407706 26.351275884313946 26.843493560976388 25.4125062106326 76-77 21.342421917983863 26.093406168226966 26.924806812481705 25.639365101307465 78-79 21.257439487867025 26.073433953297435 26.908877533250546 25.76024902558499 80-81 21.23293090040924 26.197096494271953 26.74266174553841 25.827310859780393 82-83 21.547484384066202 25.6877759850065 27.016297968770388 25.748441662156914 84-85 21.727341175574768 25.726718992805324 26.596710346368447 25.94922948525146 86-87 21.28587081528289 25.87024419775626 26.85004343878913 25.99384154817172 88-89 21.414349989892187 25.307712174542786 26.83230121737161 26.44563661819341 90-91 21.645843017198544 25.003055426437353 26.79248345060828 26.558618105755823 92-93 21.755218466979187 24.955697075049084 26.789693548845534 26.4993909091262 94-95 21.25969111128081 24.741105084007366 27.146353463136535 26.85285034157529 96-97 21.41372734757563 24.598483301709894 27.137104603210233 26.85068474750425 98-99 21.299566079257648 24.807793244579244 26.989266671942097 26.90337400422101 100 21.366833496390253 24.631945016555683 27.070712281203445 26.930509205850615 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2132.0 1 1488.5 2 1424.0 3 2124.5 4 1656.5 5 1187.0 6 1654.0 7 2666.5 8 3800.5 9 4318.5 10 3683.0 11 2912.5 12 2902.0 13 3154.0 14 3574.0 15 4042.5 16 4510.5 17 5064.0 18 5645.0 19 6135.0 20 6633.0 21 7420.5 22 8520.0 23 9809.5 24 11418.0 25 13489.5 26 16352.0 27 19862.5 28 24420.0 29 30320.5 30 37515.5 31 44857.0 32 53984.0 33 62470.5 34 70072.0 35 78199.0 36 85944.0 37 96520.0 38 102638.0 39 103186.5 40 104272.0 41 105861.0 42 109284.0 43 116836.5 44 127254.5 45 139917.5 46 151822.0 47 161304.0 48 168755.5 49 172697.5 50 174911.5 51 176693.5 52 176286.0 53 172839.0 54 166219.0 55 150582.5 56 132657.0 57 115437.5 58 97552.5 59 82504.0 60 65436.5 61 49289.0 62 37051.0 63 27372.0 64 19363.0 65 12954.5 66 8940.0 67 6446.5 68 4741.0 69 3430.0 70 2525.5 71 1919.5 72 1434.5 73 1031.5 74 703.5 75 488.5 76 334.5 77 195.0 78 112.5 79 63.5 80 43.5 81 29.5 82 20.0 83 17.5 84 8.5 85 3.5 86 4.0 87 3.5 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05102278400137797 2 0.0 3 0.0 4 0.015296820915035298 5 0.003880535584010591 6 0.0 7 2.253214210070666E-4 8 4.0057141512367394E-4 9 0.0020529285025088287 10-11 7.761071168021183E-4 12-13 1.7524999411660733E-4 14-15 3.755357016784443E-5 16-17 6.258928361307405E-5 18-19 0.0 20-21 1.251785672261481E-5 22-23 0.0 24-25 5.007142689045924E-5 26-27 0.0 28-29 0.001189196388648407 30-31 0.0011015713915901032 32-33 0.01447064237134272 34-35 0.024297159898595346 36-37 0.032283552487623594 38-39 0.0214681242792844 40-41 1.0014285378091849E-4 42-43 0.0015522142336042365 44-45 0.009738892530194321 46-47 0.014245320950335654 48-49 0.005758214092402813 50-51 0.010452410363383367 52-53 0.015835088754107732 54-55 0.009776446100362166 56-57 0.0030919106104858583 58-59 0.00834941043398408 60-61 0.0016148035172173106 62-63 0.0010765356781448736 64-65 0.019452749346943417 66-67 0.04447594493545042 68-69 0.05431498031942566 70-71 0.04804353410139564 72-73 0.041071087906899194 74-75 0.028590784754452227 76-77 0.028866177602349753 78-79 0.01220491030454944 80-81 0.0022031427831802064 82-83 0.027464177649416895 84-85 0.047517784119045815 86-87 0.04743015912198752 88-89 0.05840831946772071 90-91 0.034999927396431006 92-93 0.026224909833878027 94-95 0.007485678320123656 96-97 0.003943124867623665 98-99 0.023483499211625382 100 0.020053606469628924 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3994294.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.94765274610724 #Duplication Level Percentage of deduplicated Percentage of total 1 79.4390561055749 52.38819286528985 2 13.148673192577123 17.34248267552251 3 3.4432789602569716 6.812284955370118 4 1.3981621394540906 3.6882204502189095 5 0.7275714397627988 2.399081432873117 6 0.4466102730212498 1.767173951882972 7 0.28965942721227034 1.3371651524301795 8 0.20909988041228408 1.103171704214549 9 0.14963171604501646 0.8881074404586784 >10 0.7096083491748225 7.954798126717906 >50 0.025894888017728844 1.148478032457557 >100 0.011762939610299268 1.390956945680575 >500 5.168811581559309E-4 0.24764277433060525 >1k 3.445874344492885E-4 0.5024438348615903 >5k 4.3073429306160864E-5 0.20895391504081018 >10k+ 8.614685861232173E-5 0.8208457426501212 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15426 0.3862009156061121 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13471 0.3372560958206882 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7356 0.18416270810310909 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0014285378091849E-4 2 0.0 0.0 0.0 0.0 1.0014285378091849E-4 3 0.0 0.0 0.0 0.0 1.0014285378091849E-4 4 0.0 0.0 0.0 0.0 1.0014285378091849E-4 5 0.0 0.0 0.0 0.0 1.0014285378091849E-4 6 0.0 0.0 0.0 0.0 1.0014285378091849E-4 7 0.0 0.0 0.0 0.0 1.0014285378091849E-4 8 0.0 0.0 0.0 0.0 1.0014285378091849E-4 9 0.0 0.0 0.0 1.0014285378091849E-4 1.0014285378091849E-4 10-11 0.0 0.0 0.0 1.6273213739399255E-4 1.0014285378091849E-4 12-13 0.0 0.0 0.0 2.753928478975258E-4 3.5049998823321465E-4 14-15 0.0 0.0 0.0 2.753928478975258E-4 4.7567855545936275E-4 16-17 0.0 0.0 0.0 3.5049998823321465E-4 5.88339265962896E-4 18-19 0.0 0.0 0.0 5.007142689045924E-4 6.884821197438145E-4 20-21 0.0 0.0 0.0 0.0010139463945317995 7.761071168021183E-4 22-23 0.0 0.0 0.0 0.0035175177390547614 8.011428302473479E-4 24-25 0.0 0.0 0.0 0.00913803540750881 8.011428302473479E-4 26-27 0.0 0.0 0.0 0.019177356499045888 9.012856840282664E-4 28-29 0.0 0.0 0.0 0.05176133754801224 9.26321397473496E-4 30-31 0.0 3.755357016784443E-5 0.0 0.15448286981378936 9.26321397473496E-4 32-33 0.0 7.510714033568885E-5 0.0 0.3724938624948489 9.388392541961108E-4 34-35 0.0 7.510714033568885E-5 0.0 0.7091741369062968 9.513571109187256E-4 36-37 0.0 7.510714033568885E-5 0.0 1.202352656063875 9.513571109187256E-4 38-39 0.0 7.510714033568885E-5 0.0 1.8636334731494477 9.763928243639552E-4 40-41 0.0 7.510714033568885E-5 0.0 2.5333513256660627 9.763928243639552E-4 42-43 0.0 7.510714033568885E-5 0.0 3.1771697326235877 9.763928243639552E-4 44-45 0.0 7.510714033568885E-5 0.0 3.954265760106792 0.001051499964699644 46-47 0.0 7.510714033568885E-5 0.0 4.833983177001993 0.0011266071050353328 48-49 0.0 7.510714033568885E-5 0.0 5.710270701155197 0.0012017142453710217 50-51 0.0 7.510714033568885E-5 0.0 6.563450261798456 0.0012017142453710217 52-53 2.503571344522962E-5 7.510714033568885E-5 0.0 7.362502609973126 0.0013519285260423994 54-55 2.503571344522962E-5 7.510714033568885E-5 0.0 8.228650670181013 0.0013519285260423994 56-57 2.503571344522962E-5 7.510714033568885E-5 0.0 9.194115405626125 0.0013894820962102438 58-59 2.503571344522962E-5 7.510714033568885E-5 0.0 10.228828924460743 0.0014270356663780882 60-61 2.503571344522962E-5 7.510714033568885E-5 0.0 11.23530215852914 0.0014771070932685477 62-63 2.503571344522962E-5 7.510714033568885E-5 0.0 12.226941732381242 0.0015772499470494662 64-65 2.503571344522962E-5 7.510714033568885E-5 0.0 13.20416073528889 0.0016648749441077698 66-67 2.503571344522962E-5 7.510714033568885E-5 0.0 14.257963985625494 0.0017024285142756143 68-69 2.503571344522962E-5 7.510714033568885E-5 0.0 15.388263858393998 0.0017274642277208439 70-71 2.503571344522962E-5 7.510714033568885E-5 0.0 16.540945658982537 0.0017524999411660735 72-73 2.503571344522962E-5 7.510714033568885E-5 0.0 17.60724173032831 0.001852642794946992 74-75 2.503571344522962E-5 7.510714033568885E-5 0.0 18.63386871372012 0.0019027142218374512 76-77 2.503571344522962E-5 7.510714033568885E-5 0.0 19.721194784359888 0.0019277499352826808 78-79 2.503571344522962E-5 7.510714033568885E-5 0.0 20.84587413945994 0.0019277499352826808 80-81 2.503571344522962E-5 7.510714033568885E-5 0.0 21.979428654976324 0.0019402677920052956 82-83 2.503571344522962E-5 7.510714033568885E-5 0.0 23.08914917129285 0.0020529285025088287 84-85 2.503571344522962E-5 7.510714033568885E-5 0.0 24.14140020739585 0.0020779642159540583 86-87 2.503571344522962E-5 7.510714033568885E-5 0.0 25.194477422042546 0.002165589213012362 88 2.503571344522962E-5 7.510714033568885E-5 0.0 25.99670930582476 0.0022532142100706656 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7395 0.0 56.329453 1 GTATCAA 12765 0.0 38.636223 1 GTGGTAT 2635 0.0 35.6853 1 TACCTGG 2670 0.0 35.198112 2 ATCAACG 15535 0.0 31.397686 3 CAACGCA 15605 0.0 31.285 5 TATCAAC 15920 0.0 30.873743 2 TCAACGC 16625 0.0 29.396397 4 AACGCAG 16990 0.0 28.98251 6 TGGTATC 3385 0.0 26.514006 2 GTACATG 13565 0.0 26.479729 1 ACCTGGG 3490 0.0 26.390171 3 ACGCAGA 18530 0.0 26.218822 7 TACATGG 13670 0.0 25.711876 2 CGCAGAG 18815 0.0 25.646862 8 GTACTAG 1340 0.0 25.612858 1 ACATGGG 13250 0.0 25.534548 3 CATGGGG 10135 0.0 23.785706 4 GCAGAGT 20205 0.0 23.161598 9 GAGTACT 13340 0.0 22.206268 12-13 >>END_MODULE