FastQCFastQC Report
Mon 6 Jun 2016
ERR1211460_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211460_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2720724
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123290.4531514405724359No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101960.37475319069482976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54980.20207856438212768No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31560.11599853568388413No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28100.10328133246885755No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52500.050.074291
TACCTGG19600.046.347372
GTATCAA93900.040.138791
ACCTGGG24800.036.7751433
ATCAACG112650.032.9268463
CAACGCA113500.032.8873565
TATCAAC117950.031.644562
TCAACGC119250.031.1433324
AACGCAG121050.030.9138156
GTACATG93950.028.08221
ACGCAGA132300.027.9297497
CGCAGAG134900.027.2146958
GTACTAG10250.027.1186491
TACATGG95900.027.0431212
ACATGGG94200.026.5500343
CATGGGG65100.026.068894
ATGGGGG49600.025.8752945
CCTGGGG25500.025.4410634
GCAGAGT144400.024.6420659
CGCCTAC4950.023.7431937
Produced by FastQC (version 0.11.5)