##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211460_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2720724 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.461648076026822 31.0 31.0 34.0 30.0 34.0 2 31.508623072388087 31.0 31.0 34.0 28.0 34.0 3 31.57117884798311 31.0 31.0 34.0 28.0 34.0 4 35.21165175151908 37.0 35.0 37.0 32.0 37.0 5 35.26963300944895 37.0 35.0 37.0 33.0 37.0 6 35.33481271896745 37.0 35.0 37.0 32.0 37.0 7 35.27199157283135 37.0 35.0 37.0 32.0 37.0 8 35.280251874133505 37.0 35.0 37.0 32.0 37.0 9 36.85961163278598 39.0 37.0 39.0 33.0 39.0 10-11 36.790122408594186 39.0 37.0 39.0 32.0 39.0 12-13 36.70671795448565 39.0 37.0 39.0 32.0 39.0 14-15 37.90497529334104 40.0 38.0 41.0 32.0 41.0 16-17 37.90139334236034 40.0 37.5 41.0 32.0 41.0 18-19 37.90925595540011 40.0 37.5 41.0 32.0 41.0 20-21 37.90041823426411 40.0 37.5 41.0 32.0 41.0 22-23 37.77022917429332 40.0 37.0 41.0 32.0 41.0 24-25 37.68593433218511 40.0 37.0 41.0 32.0 41.0 26-27 37.55242372986014 40.0 37.0 41.0 32.0 41.0 28-29 37.4255049391265 40.0 36.5 41.0 31.5 41.0 30-31 37.25703544350695 39.5 36.0 41.0 31.0 41.0 32-33 37.04758953866691 39.0 36.0 41.0 31.0 41.0 34-35 36.91926248307436 39.0 36.0 41.0 30.0 41.0 36-37 36.783813426132156 39.0 35.0 41.0 30.0 41.0 38-39 36.628059295981515 39.0 35.0 41.0 30.0 41.0 40-41 36.46139795877862 39.0 35.0 40.5 30.0 41.0 42-43 36.25621250079023 39.0 35.0 40.0 29.0 41.0 44-45 36.10753442833599 39.0 35.0 40.0 28.5 41.0 46-47 35.869646829299846 38.5 35.0 40.0 27.5 41.0 48-49 35.61000674820379 38.0 34.5 40.0 26.5 41.0 50-51 34.932399243730714 37.5 33.5 39.5 25.5 40.5 52-53 35.22355226035423 38.0 34.0 40.0 26.5 40.5 54-55 35.62770185435936 39.0 35.0 40.0 27.0 41.0 56-57 35.55127348455778 38.5 34.5 40.0 26.5 41.0 58-59 35.350736054079725 38.0 34.0 40.0 26.0 41.0 60-61 35.128907967144045 38.0 34.0 40.0 26.0 41.0 62-63 34.847415430598616 37.5 34.0 40.0 26.0 41.0 64-65 34.64273020710664 37.0 34.0 40.0 26.0 41.0 66-67 34.446878661709164 37.0 33.5 40.0 25.5 41.0 68-69 34.10546347222284 36.0 33.0 39.0 25.0 41.0 70-71 33.72346717270844 35.5 33.0 39.0 24.0 41.0 72-73 33.32608177088157 35.0 32.5 38.5 23.5 40.5 74-75 32.92481045486422 35.0 32.0 37.5 22.5 40.0 76-77 32.51262531590856 35.0 32.0 37.0 21.5 39.0 78-79 32.09419386163389 35.0 31.0 36.5 20.0 39.0 80-81 31.674428203669315 35.0 31.0 36.0 19.5 38.5 82-83 31.274137692761194 35.0 31.0 35.5 18.5 37.0 84-85 30.90945535085514 34.5 30.0 35.0 18.0 37.0 86-87 30.548952778745658 34.0 30.0 35.0 16.0 36.5 88-89 30.20989707151479 34.0 29.0 35.0 14.0 36.0 90-91 29.88435320892527 34.0 29.0 35.0 11.5 36.0 92-93 29.561108182968944 34.0 29.0 35.0 10.0 35.5 94-95 29.245553757014676 34.0 27.5 35.0 10.0 35.0 96-97 28.928321101295097 34.0 27.0 35.0 10.0 35.0 98-99 28.59279423418178 34.0 25.5 35.0 8.0 35.0 100 27.341015112153972 32.0 24.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3309524116836471 1101 2 -0.3211078507182279 1101 3 -0.27201355681888373 1101 4 -0.24369013370063186 1101 5 -0.2617265314259498 1101 6 -0.31668244497560494 1101 7 -0.2442937054315948 1101 8 -0.20703816164724032 1101 9 -0.43266484874026645 1101 10-11 -0.4234627122954109 1101 12-13 -0.4413285498611401 1101 14-15 -0.6212688204374359 1101 16-17 -0.49820365617277673 1101 18-19 -0.511567363456237 1101 20-21 -0.5221693997536789 1101 22-23 -0.5267140817202005 1101 24-25 -0.6299471090059541 1101 26-27 -0.5658843857467062 1101 28-29 -0.5525338439879803 1101 30-31 -0.4764444947779367 1101 32-33 -0.5951819481349006 1101 34-35 -0.5291458536353062 1101 36-37 -0.5143861408320802 1101 38-39 -0.6183564748317423 1101 40-41 -0.7230284749696168 1101 42-43 -0.7382861161711958 1101 44-45 -0.8195520424081124 1101 46-47 -0.9879561354612036 1101 48-49 -0.9867473534102018 1101 50-51 -1.1225334356617154 1101 52-53 -0.8242923782456799 1101 54-55 -0.6715393838904404 1101 56-57 -0.8010752628021436 1101 58-59 -0.60220432346091 1101 60-61 -0.6080734206339287 1101 62-63 -0.671054738499052 1101 64-65 -0.7119894405815188 1101 66-67 -1.013487385429535 1101 68-69 -0.9711855047834419 1101 70-71 -0.8431916217397486 1101 72-73 -1.0211312624671791 1101 74-75 -0.9925113727116894 1101 76-77 -1.0771930817407664 1101 78-79 -1.116943814073565 1101 80-81 -1.2017063939789274 1101 82-83 -1.250806648233901 1101 84-85 -1.2354311989313302 1101 86-87 -1.412525086622896 1101 88-89 -1.4350712786254505 1101 90-91 -1.4255441952718009 1101 92-93 -1.4738340422119052 1101 94-95 -1.5830003342209942 1101 96-97 -1.9601854619904096 1101 98-99 -2.0538539334712844 1101 100 -2.0582256919594286 1102 1 -0.37916393987897123 1102 2 -0.32590466728359146 1102 3 -0.48146288875389587 1102 4 -0.4720854742875602 1102 5 -0.24686182432159853 1102 6 -0.26541147883926897 1102 7 -0.34593294564175636 1102 8 -0.34621514288630806 1102 9 -0.5803046287845746 1102 10-11 -0.5029081144839935 1102 12-13 -0.49350423203321725 1102 14-15 -0.6965778690064823 1102 16-17 -0.7426968702762764 1102 18-19 -0.7269300165516057 1102 20-21 -0.7701537248096102 1102 22-23 -0.7041554913382129 1102 24-25 -0.689577489394722 1102 26-27 -0.7160908067289284 1102 28-29 -0.7980911013002299 1102 30-31 -0.8094390149918382 1102 32-33 -0.7876048076334285 1102 34-35 -0.8685630076342576 1102 36-37 -0.8250654655892689 1102 38-39 -0.7824607104248145 1102 40-41 -0.8244147185254889 1102 42-43 -0.9606423254215315 1102 44-45 -0.955968890868057 1102 46-47 -1.0408768017013799 1102 48-49 -1.0904625424873444 1102 50-51 -0.9550610657299217 1102 52-53 -0.7806027300915659 1102 54-55 -0.8120297618407264 1102 56-57 -0.7407641797352014 1102 58-59 -0.5407922799134255 1102 60-61 -0.6375825455958193 1102 62-63 -0.6214418112362807 1102 64-65 -0.6719951627586482 1102 66-67 -0.5427618914141021 1102 68-69 -0.5925699801962949 1102 70-71 -0.694670117995642 1102 72-73 -0.8729565867765032 1102 74-75 -0.9270132510398099 1102 76-77 -0.9886351043797958 1102 78-79 -0.9920849862066561 1102 80-81 -1.0003056749664836 1102 82-83 -1.130001029166067 1102 84-85 -1.2401778209604828 1102 86-87 -1.4463244681290597 1102 88-89 -1.4789686673923335 1102 90-91 -1.3095999979949688 1102 92-93 -1.4224421234350828 1102 94-95 -1.572724379233513 1102 96-97 -1.6989528558139888 1102 98-99 -1.7634358761326112 1102 100 -1.6319850247024092 1103 1 -0.4001530501647359 1103 2 -0.3473417574243811 1103 3 -0.49587398709352826 1103 4 -0.29363202632099217 1103 5 -0.30657370201662104 1103 6 -0.38376303921107535 1103 7 -0.4861607725491126 1103 8 -0.37909008724179216 1103 9 -0.4058220397035228 1103 10-11 -0.4250106963893998 1103 12-13 -0.5356290385231972 1103 14-15 -0.7060067071490508 1103 16-17 -0.6280347072162655 1103 18-19 -0.6667847760676437 1103 20-21 -0.5608914049483431 1103 22-23 -0.6617895863092826 1103 24-25 -0.749475651630874 1103 26-27 -0.7689702026454555 1103 28-29 -0.6458294425289921 1103 30-31 -0.5952088028450575 1103 32-33 -0.7091809260050894 1103 34-35 -0.5656140976022996 1103 36-37 -0.8278521760185455 1103 38-39 -0.8102618718171612 1103 40-41 -0.9514400957454541 1103 42-43 -1.0695912919285178 1103 44-45 -0.8941561421287716 1103 46-47 -0.9855977984524031 1103 48-49 -0.8849637018864556 1103 50-51 -0.7452878129676321 1103 52-53 -0.4399461912799367 1103 54-55 -0.665208392354522 1103 56-57 -0.6031142717562759 1103 58-59 -0.45602567127208005 1103 60-61 -0.5669246447393874 1103 62-63 -0.6501366068963463 1103 64-65 -0.5567744782813051 1103 66-67 -0.5949836854596384 1103 68-69 -0.6438166664409621 1103 70-71 -0.6425302127408088 1103 72-73 -0.7491629099864099 1103 74-75 -0.7112621165561563 1103 76-77 -0.8365610589738779 1103 78-79 -0.8474845802508675 1103 80-81 -0.9252075911309667 1103 82-83 -0.7503395139078428 1103 84-85 -0.9296338501680381 1103 86-87 -1.0540975913309971 1103 88-89 -1.0836797644031435 1103 90-91 -1.115518747039875 1103 92-93 -1.17977324649085 1103 94-95 -1.1609014151582748 1103 96-97 -1.2541250548186973 1103 98-99 -1.3735369332122538 1103 100 -1.3113473884148767 1104 1 -0.4793811342150782 1104 2 -0.4283557749053237 1104 3 -0.47018436248384887 1104 4 -0.32527430784328004 1104 5 -0.30076115173503837 1104 6 -0.259805487088677 1104 7 -0.4028378932385337 1104 8 -0.4129996417640669 1104 9 -0.5375781791692731 1104 10-11 -0.4759899087924069 1104 12-13 -0.5253189290286713 1104 14-15 -0.7521242876336558 1104 16-17 -0.7584012002552427 1104 18-19 -0.7796466329748029 1104 20-21 -0.7918009390170866 1104 22-23 -0.7235469529863963 1104 24-25 -0.669089727745245 1104 26-27 -0.6689971331171094 1104 28-29 -0.8336916403281904 1104 30-31 -0.8519410798675722 1104 32-33 -0.8686877233924903 1104 34-35 -0.8367390072658978 1104 36-37 -1.0081598651795431 1104 38-39 -0.9744767292561107 1104 40-41 -0.957770290599349 1104 42-43 -0.9047645332664445 1104 44-45 -0.9501834543995287 1104 46-47 -0.758370580267858 1104 48-49 -0.9325593902838563 1104 50-51 -0.8269336738564093 1104 52-53 -0.8545714827426352 1104 54-55 -0.781332415698806 1104 56-57 -0.5298858877209369 1104 58-59 -0.5385152399754674 1104 60-61 -0.7081896269448222 1104 62-63 -0.6279005596077951 1104 64-65 -0.5525917328731182 1104 66-67 -0.5511340597591072 1104 68-69 -0.566248215318069 1104 70-71 -0.5402193055588924 1104 72-73 -0.5596482379927394 1104 74-75 -0.7629084546937932 1104 76-77 -0.7410282787947011 1104 78-79 -0.6716471789391143 1104 80-81 -0.720255622820293 1104 82-83 -0.8286073050637413 1104 84-85 -0.9225005136530484 1104 86-87 -1.0939857105613982 1104 88-89 -0.9318496854525478 1104 90-91 -0.9923636547944881 1104 92-93 -1.1174179551645054 1104 94-95 -1.0356926187799154 1104 96-97 -0.9886640665044766 1104 98-99 -1.2886977660797 1104 100 -1.3021235314259094 1105 1 -0.4073785623830979 1105 2 -0.47980210729845396 1105 3 -0.4578030593146174 1105 4 -0.30132040696220486 1105 5 -0.3259512120567791 1105 6 -0.4088583356510682 1105 7 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1105 70-71 -0.7167099826679788 1105 72-73 -0.719589531727614 1105 74-75 -0.7160720341928055 1105 76-77 -0.8983565460067524 1105 78-79 -0.8549202551538713 1105 80-81 -0.7873247036488316 1105 82-83 -1.0203738886951257 1105 84-85 -0.912711074318878 1105 86-87 -0.9500083530218255 1105 88-89 -0.9907733931554397 1105 90-91 -1.090631060701302 1105 92-93 -0.9792218885165802 1105 94-95 -1.258354787971566 1105 96-97 -1.3157248438051532 1105 98-99 -1.4454585178559114 1105 100 -1.2068310178478647 1106 1 -0.5254962423324621 1106 2 -0.5596812641291393 1106 3 -0.6117672147238942 1106 4 -0.37163624206640833 1106 5 -0.373145856885607 1106 6 -0.3683362944161246 1106 7 -0.4704190068411549 1106 8 -0.4495624694693774 1106 9 -0.6742868057495386 1106 10-11 -0.7081787859806141 1106 12-13 -0.6880528435648614 1106 14-15 -0.869917838440351 1106 16-17 -0.7667139227959652 1106 18-19 -0.8161341857970044 1106 20-21 -0.8076171594408308 1106 22-23 -0.8073398537704861 1106 24-25 -0.8610620744118052 1106 26-27 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2311 40-41 1.3558875632451972 2311 42-43 1.3494522313000559 2311 44-45 1.3084870180544357 2311 46-47 1.3480458999666638 2311 48-49 1.32888690072663 2311 50-51 1.247181370391381 2311 52-53 1.165703702128262 2311 54-55 1.077532019659749 2311 56-57 1.1428457932541676 2311 58-59 1.1155984817979672 2311 60-61 1.1411309518581163 2311 62-63 1.1418056857773138 2311 64-65 1.0860242426170217 2311 66-67 1.0919039393372003 2311 68-69 1.0594626146447936 2311 70-71 1.0474786523202937 2311 72-73 1.09731982425172 2311 74-75 1.1963124977081492 2311 76-77 1.1512038374948759 2311 78-79 1.0859749570773758 2311 80-81 1.223668709605679 2311 82-83 1.2646271365765642 2311 84-85 1.2453669413552717 2311 86-87 1.2336628198389796 2311 88-89 1.2981671760954114 2311 90-91 1.3123592317261945 2311 92-93 1.2917941807417748 2311 94-95 1.3303324641502492 2311 96-97 1.5814752892301023 2311 98-99 1.4979085994301684 2311 100 1.6756230824887233 2312 1 0.7373252765799272 2312 2 0.7568783254808231 2312 3 0.7808501370402077 2312 4 0.5807567211915838 2312 5 0.5584798075836304 2312 6 0.5058664748278616 2312 7 0.5690498781553615 2312 8 0.7036114540267562 2312 9 0.9113762552448108 2312 10-11 0.9038661488382189 2312 12-13 0.8954654141904257 2312 14-15 1.1939808890802013 2312 16-17 1.155809914882191 2312 18-19 1.0925271214158556 2312 20-21 1.114588407581337 2312 22-23 1.1282151084512648 2312 24-25 1.101300250910164 2312 26-27 1.2018730670418662 2312 28-29 1.1749814173057302 2312 30-31 1.1360186903128664 2312 32-33 1.2599056763220204 2312 34-35 1.347886206490152 2312 36-37 1.2900048525181305 2312 38-39 1.3380460137800583 2312 40-41 1.332967630870101 2312 42-43 1.3568001977510136 2312 44-45 1.3055332301070521 2312 46-47 1.4049000462257055 2312 48-49 1.4106436294089235 2312 50-51 1.2880325164124997 2312 52-53 1.1686301492609275 2312 54-55 1.2248031711607936 2312 56-57 1.1198983240728495 2312 58-59 1.1386193007994265 2312 60-61 1.0735444578678042 2312 62-63 1.0157526234296768 2312 64-65 1.0738879097513205 2312 66-67 1.116021526791549 2312 68-69 1.0916101699839729 2312 70-71 1.056011877387924 2312 72-73 1.0485599310906082 2312 74-75 1.1089263540722527 2312 76-77 1.14515024793009 2312 78-79 1.2028553230797456 2312 80-81 1.0284153102310505 2312 82-83 1.2771791184230494 2312 84-85 1.3667355127849703 2312 86-87 1.4311954044063775 2312 88-89 1.5612615644274896 2312 90-91 1.5764578805215699 2312 92-93 1.613638884130193 2312 94-95 1.662426543207733 2312 96-97 1.8224389539733075 2312 98-99 1.705598590653672 2312 100 1.8134214746528272 2313 1 0.6073713463427133 2313 2 0.6226783153196642 2313 3 0.6374551534995341 2313 4 0.46558990590335725 2313 5 0.4598301322443561 2313 6 0.5186490966756381 2313 7 0.6041125941165006 2313 8 0.5738026414983963 2313 9 0.7572346775904819 2313 10-11 0.6270360159781418 2313 12-13 0.7534435011244085 2313 14-15 0.9443829556498216 2313 16-17 0.9343735765903247 2313 18-19 0.9183273273253292 2313 20-21 0.9694977075533657 2313 22-23 0.9999071108595672 2313 24-25 0.9659576942806822 2313 26-27 1.062460936836537 2313 28-29 0.9867764503674579 2313 30-31 1.003718641784566 2313 32-33 1.0446213653274654 2313 34-35 1.000426671555907 2313 36-37 0.9245845922172506 2313 38-39 1.0795715191663504 2313 40-41 1.0821881706612615 2313 42-43 1.0247673760379001 2313 44-45 1.024844046544537 2313 46-47 1.0690083198038351 2313 48-49 1.1675139455669807 2313 50-51 1.1291386987772825 2313 52-53 0.9871407451895706 2313 54-55 0.9236193960798076 2313 56-57 0.9133280833059274 2313 58-59 0.8055886123458365 2313 60-61 0.7231469463007301 2313 62-63 0.7381821566645499 2313 64-65 0.8041994952834628 2313 66-67 0.8192899746978028 2313 68-69 0.8298803084969322 2313 70-71 0.8013533653437364 2313 72-73 0.8545556575638784 2313 74-75 0.8129859416810064 2313 76-77 0.9734859259217785 2313 78-79 0.9421615786749484 2313 80-81 0.9552619695746749 2313 82-83 1.0324705130305176 2313 84-85 1.052996282008099 2313 86-87 1.1855990115011998 2313 88-89 1.265693289903325 2313 90-91 1.2405515547326047 2313 92-93 1.3894572808510581 2313 94-95 1.5183624706734058 2313 96-97 1.5169606290696471 2313 98-99 1.6273477349847632 2313 100 1.752005441160712 2314 1 0.413070640058784 2314 2 0.45942394009062326 2314 3 0.4311552905618221 2314 4 0.30876715719496417 2314 5 0.35979837707265716 2314 6 0.4013211480115615 2314 7 0.4004398509847178 2314 8 0.4144737538890979 2314 9 0.5445765548065395 2314 10-11 0.5006477461977639 2314 12-13 0.284648885941877 2314 14-15 0.6486015023745679 2314 16-17 0.6615905377797304 2314 18-19 0.7995672463552026 2314 20-21 0.7978321024802426 2314 22-23 0.7226479638323866 2314 24-25 0.8051524876182441 2314 26-27 0.7780124115252178 2314 28-29 0.8631630527234151 2314 30-31 0.750299773283956 2314 32-33 0.7533283406825646 2314 34-35 0.8412421087935016 2314 36-37 0.8448999540501987 2314 38-39 0.8899105139613965 2314 40-41 0.8319330547252406 2314 42-43 0.8982718352866073 2314 44-45 0.9869420999932714 2314 46-47 0.9663346232758272 2314 48-49 1.0001666918335275 2314 50-51 0.9520876833713956 2314 52-53 0.8385406220965947 2314 54-55 0.7982421413051313 2314 56-57 0.7883085837037029 2314 58-59 0.783784907476992 2314 60-61 0.7280393548137809 2314 62-63 0.834434231218431 2314 64-65 0.74087914562827 2314 66-67 0.8192743194352019 2314 68-69 0.8312487081363216 2314 70-71 0.828905793799187 2314 72-73 0.9052816726825981 2314 74-75 0.9980658835657081 2314 76-77 1.0198485692415389 2314 78-79 1.0540743812708016 2314 80-81 1.1140977627295605 2314 82-83 0.9225182310119777 2314 84-85 1.090089342447044 2314 86-87 1.3316497352930874 2314 88-89 1.4366743246179894 2314 90-91 1.479300793738627 2314 92-93 1.5978450370647188 2314 94-95 1.6258308461166173 2314 96-97 1.789579056036274 2314 98-99 1.7933274227910374 2314 100 1.7617741397507736 2315 1 0.3594840098570806 2315 2 0.3899696627084026 2315 3 0.4815935515189622 2315 4 0.3611538804312744 2315 5 0.28914331238244273 2315 6 -0.35162452234908415 2315 7 0.20912379689858085 2315 8 0.23098315321901453 2315 9 0.46249156195403884 2315 10-11 0.5595223303019097 2315 12-13 0.5646992485814053 2315 14-15 0.750761871921263 2315 16-17 0.5673390781276808 2315 18-19 0.6807988615004064 2315 20-21 0.7507841729315956 2315 22-23 0.5876097472797284 2315 24-25 0.7645735796057664 2315 26-27 0.8649381882558984 2315 28-29 0.9054849510519389 2315 30-31 0.9743012755005651 2315 32-33 0.9249156089565886 2315 34-35 0.9341742870234384 2315 36-37 0.9591006373017024 2315 38-39 1.0662689310138376 2315 40-41 1.0638928980735542 2315 42-43 1.201756521815085 2315 44-45 1.144252764269865 2315 46-47 1.221027773699788 2315 48-49 1.2023594227742365 2315 50-51 1.191879230568226 2315 52-53 1.0773422680555527 2315 54-55 0.9131462623121038 2315 56-57 0.8734883769534818 2315 58-59 0.8067769032259946 2315 60-61 0.6336414061528899 2315 62-63 0.8179559784097421 2315 64-65 0.831189531540538 2315 66-67 0.9414550002463926 2315 68-69 0.9523230183834883 2315 70-71 1.0390153577179575 2315 72-73 1.041934613634325 2315 74-75 0.9977442063792381 2315 76-77 1.040094820434625 2315 78-79 1.091656294948983 2315 80-81 1.1857578705504004 2315 82-83 1.3694243285641505 2315 84-85 1.3755101030736228 2315 86-87 1.4231917645215155 2315 88-89 1.5441810587987206 2315 90-91 1.6670974141738064 2315 92-93 1.7817684141575327 2315 94-95 1.8744447026739444 2315 96-97 1.948399948045175 2315 98-99 2.1740536772660377 2315 100 2.1515390477805028 2316 1 0.04003711942479171 2316 2 -0.07266270841932965 2316 3 0.12001872217387088 2316 4 0.10286911783255448 2316 5 0.1222857949478211 2316 6 0.16302120332007775 2316 7 0.11584504866893042 2316 8 0.13459455268058917 2316 9 0.30532304484624007 2316 10-11 0.1406587559359096 2316 12-13 0.27871287718674154 2316 14-15 0.4799127317012122 2316 16-17 0.426198967158129 2316 18-19 0.3829131379958852 2316 20-21 0.37772359350815776 2316 22-23 0.21355133224756884 2316 24-25 0.32929288880160357 2316 26-27 0.4958091006703924 2316 28-29 0.6111653291547938 2316 30-31 0.5968807351325154 2316 32-33 0.6554022542027766 2316 34-35 0.6893553140528823 2316 36-37 0.6916147268288526 2316 38-39 0.7538079092991552 2316 40-41 0.7098189754076643 2316 42-43 0.8098570643613883 2316 44-45 0.6956004986168054 2316 46-47 0.7086577299897385 2316 48-49 0.8232902512008025 2316 50-51 0.6132849473579256 2316 52-53 0.4063311446798039 2316 54-55 0.3790892762182523 2316 56-57 0.520591756732415 2316 58-59 0.47358077175788793 2316 60-61 0.5806130976276123 2316 62-63 0.624312876397056 2316 64-65 0.632016981972221 2316 66-67 0.5903797241669295 2316 68-69 0.6528108368542931 2316 70-71 0.5335507306101164 2316 72-73 0.6608296297404621 2316 74-75 0.6758653597890216 2316 76-77 0.8558779113369326 2316 78-79 0.9102037273328847 2316 80-81 1.0525503211685638 2316 82-83 1.100539426316871 2316 84-85 1.1722927745544993 2316 86-87 1.3234776116521338 2316 88-89 1.3804810957455231 2316 90-91 1.5465332078211844 2316 92-93 1.594477418686214 2316 94-95 1.8042015954110546 2316 96-97 1.9299511820676365 2316 98-99 2.03699270942964 2316 100 1.965211183916697 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 16.0 1 162.0 2 67.0 3 63.0 4 35.0 5 35.0 6 47.0 7 178.0 8 689.0 9 1382.0 10 1879.0 11 2467.0 12 3210.0 13 3837.0 14 4725.0 15 5933.0 16 8210.0 17 11204.0 18 14885.0 19 18705.0 20 22943.0 21 27481.0 22 29738.0 23 29757.0 24 29171.0 25 30297.0 26 33794.0 27 39380.0 28 46389.0 29 55140.0 30 66241.0 31 81996.0 32 102063.0 33 131254.0 34 177648.0 35 240456.0 36 345417.0 37 505542.0 38 504295.0 39 143820.0 40 173.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.61529450638588 19.684004743133674 12.574829785376519 23.125870965103932 2 16.067082373208276 21.33296032628301 40.27051600126626 22.329441299242454 3 18.601018421061337 27.571998457405684 30.621930941331787 23.205052180201193 4 12.452777888066867 17.574566453588144 39.355374975552586 30.6172806827924 5 12.411056527496832 37.9818499065804 35.201431183836974 14.40566238208579 6 28.254907953011337 38.03484763399945 19.567712522281298 14.142531890707923 7 25.58220103823599 33.64388468703051 23.658136066656002 17.115778208077497 8 25.030817388950755 35.07059315015497 21.74269760271605 18.15589185817823 9 26.84392171743914 15.942475025592149 21.587345711697083 35.62625754527163 10-11 24.100962008708894 26.314129930377366 29.556064714408954 20.028843346504786 12-13 24.815618659952364 24.94440752068114 28.853687213132233 21.386286606234265 14-15 22.532265600845832 26.23284244255019 26.996548360995337 24.238343595608637 16-17 21.242440840964484 28.708096066112542 28.000062495175005 22.04940059774797 18-19 20.730339732524673 28.128251697890626 30.218014137493256 20.923394432091452 20-21 21.780576663615342 27.544639410616405 29.924672717848043 20.75011120792021 22-23 21.92138116504551 27.31968209821794 29.551262903156132 21.207673833580422 24-25 21.34178004900904 27.30620686181683 30.0080088658163 21.34400422335784 26-27 21.257666309705126 27.438174150911284 29.830055426458753 21.474104112924838 28-29 21.018710686245985 27.93285820607323 29.821719747950826 21.22671135972996 30-31 21.811462806947567 27.501656567958804 29.842945409997125 20.843935215096504 32-33 21.661778506125444 27.638802466537314 29.485966490901223 21.21345253643602 34-35 21.731496992382933 27.55662156451234 29.809719484538753 20.90216195856597 36-37 21.543836171677462 27.29086034501299 29.684407504662154 21.480895978647393 38-39 21.34758656439045 27.431128071365418 29.9196900096872 21.301595354556934 40-41 21.360792185495225 26.848132584823187 30.3545680809685 21.436507148713087 42-43 21.023371516221967 27.087638038569178 29.81611034555784 22.072880099651016 44-45 20.59528528261316 27.510135480469206 30.098479438928628 21.796099797989008 46-47 20.645987572834386 27.07547197563105 29.791423211250784 22.487117240283787 48-49 20.791807257356847 27.100044803946428 29.798816124199405 22.30933181449732 50-51 20.666146190169023 27.226117013092367 29.612949680756234 22.494787115982373 52-53 20.382671493418986 27.586893492157756 29.445168620716917 22.585266393706345 54-55 20.30372776355697 27.311156963332746 29.653150189190974 22.73196508391931 56-57 20.754958766194072 27.124480957804636 29.194435481572796 22.926124794428496 58-59 20.474075719293573 27.112135995696175 29.461002802416235 22.95278548259402 60-61 20.849695513182848 27.192587245505027 29.028643947753523 22.9290732935586 62-63 20.90626440698903 26.83108005267581 29.117577206755502 23.145078333579654 64-65 20.683643540070015 26.595083701775064 29.032791742983676 23.688481015171245 66-67 20.962932936990498 26.591056365797233 28.57425959168967 23.871751105522605 68-69 20.759188406734943 26.693662663165412 28.697625474945454 23.84952345515419 70-71 21.06253113871643 26.405980839692834 28.75355050309135 23.777937518499385 72-73 21.366355710645433 26.489224399445497 28.422471199491095 23.72194869041797 74-75 21.542671012167034 26.533158336871864 28.29113749538153 23.633033155579575 76-77 21.510009319116318 26.41772801046976 28.419297739333103 23.652964931080817 78-79 21.673166434179812 26.49616732142243 28.22631800194506 23.604348242452694 80-81 21.87620110055965 26.400289100481718 28.151288076094012 23.57222172286462 82-83 22.322661568541168 25.99845490182671 28.402363709786965 23.276519819845156 84-85 22.505867714063584 26.179488640436404 27.916865014088028 23.397778631411985 86-87 22.28671352706702 26.21670487560514 28.202718069865657 23.29386352746219 88-89 22.844062953378305 25.58850835976059 28.097548584140856 23.469880102720257 90-91 23.554843674953307 25.34966876291159 27.877680220970547 23.21780734116455 92-93 23.76588578721211 25.229641864750946 27.809950115057593 23.194522232979352 94-95 23.48246760969543 24.990317328755502 27.93629418115115 23.590920880397917 96-97 23.828192065657642 24.753561424812567 27.75532720182614 23.662919307703646 98-99 23.891549412545224 24.809943264698134 27.510472307646776 23.78803501510987 100 24.58675976788912 24.590840789777 27.242916761492214 23.57948268084166 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1765.0 1 1158.5 2 1219.0 3 1858.5 4 1343.0 5 940.0 6 1294.0 7 2098.5 8 3045.0 9 3538.0 10 3026.0 11 2413.5 12 2502.0 13 2694.5 14 2937.0 15 3217.0 16 3395.5 17 3653.5 18 4050.0 19 4427.5 20 4802.0 21 5321.0 22 6152.5 23 7196.5 24 8352.0 25 9833.5 26 12147.0 27 15070.0 28 18089.5 29 22586.0 30 28998.0 31 35080.5 32 42267.5 33 50392.5 34 58481.0 35 65922.0 36 73347.5 37 82267.5 38 86976.0 39 87920.5 40 87490.0 41 86672.0 42 87568.0 43 90395.0 44 95326.5 45 101309.0 46 105892.0 47 109728.0 48 112695.5 49 113445.0 50 112465.5 51 110204.5 52 106178.5 53 99936.0 54 92099.5 55 82639.5 56 73161.5 57 61844.5 58 50798.5 59 41962.5 60 32958.0 61 24779.0 62 18659.5 63 14217.5 64 10295.5 65 7066.5 66 4993.0 67 3801.0 68 2878.5 69 2138.5 70 1620.5 71 1153.5 72 896.5 73 687.0 74 508.0 75 374.5 76 262.5 77 188.0 78 126.5 79 89.5 80 74.0 81 54.0 82 34.0 83 27.5 84 27.5 85 22.5 86 14.5 87 11.5 88 10.5 89 9.5 90 7.0 91 3.5 92 5.0 93 7.5 94 5.5 95 3.5 96 2.5 97 1.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22284509564365956 2 0.14937200539268225 3 0.023192356152259473 4 0.022052953552069227 5 0.02712513286904515 6 0.0556837077189748 7 0.06207906424907488 8 0.054397285428437435 9 0.04190061174893153 10-11 0.04432643663965915 12-13 0.04250706797161344 14-15 0.02084004110670542 16-17 0.018892030209605972 18-19 0.018763387980552236 20-21 0.021832424016548536 22-23 0.025783578194627607 24-25 0.022549145006990786 26-27 0.019957922964622652 28-29 0.019866035658155697 30-31 0.018065044451403375 32-33 0.026334902033429336 34-35 0.02622463726566899 36-37 0.025048479742891965 38-39 0.02348639553295373 40-41 0.02403771937175546 42-43 0.030580095592202667 44-45 0.03932776716785679 46-47 0.04403239725896489 48-49 0.0403385275389933 50-51 0.02868721707898339 52-53 0.04259895527808039 54-55 0.04107362599072894 56-57 0.04305839181041517 58-59 0.04723007552401493 60-61 0.024092851755635632 62-63 0.02536089658487961 64-65 0.026095995036615253 66-67 0.031480591195578826 68-69 0.03447611738640156 70-71 0.03818836456766655 72-73 0.04241518066514648 74-75 0.024589043210557188 76-77 0.018708255596672062 78-79 0.01832232890951085 80-81 0.0206746439550649 82-83 0.018689878135378674 84-85 0.012092369531051294 86-87 0.013764718508749876 88-89 0.019130937206420055 90-91 0.019939545503329263 92-93 0.020748153800238468 94-95 0.02420311652339598 96-97 0.024809572746077882 98-99 0.029863374601760417 100 0.03002877175340093 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2720724.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.99206177655041 #Duplication Level Percentage of deduplicated Percentage of total 1 83.63102023530206 59.37138554980502 2 11.023579555362288 15.651732815859953 3 2.6829969042023754 5.714144459082857 4 0.9897421044689693 2.8105533049325637 5 0.48860418533556793 1.7343509254811866 6 0.2883542410265187 1.2282517255490955 7 0.18513909941652434 0.9200384468122963 8 0.1331044916586107 0.7559489835651545 9 0.09215905134020333 0.5888304959410796 >10 0.4324159872507234 5.326976281030817 >50 0.0338101380256727 1.689800788132955 >100 0.017961404657190726 2.3288325198914004 >500 6.675611719897578E-4 0.3041747263239935 >1k 2.781504883179473E-4 0.4682679472744334 >5k 5.563009766358946E-5 0.21713225724169982 >10k+ 1.1126019532717893E-4 0.8895787730755343 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12329 0.4531514405724359 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10196 0.37475319069482976 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5498 0.20207856438212768 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3156 0.11599853568388413 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2810 0.10328133246885755 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.188730646695512E-5 0.0 12-13 0.0 0.0 0.0 1.6539715164051922E-4 0.0 14-15 0.0 0.0 0.0 2.5728445810747436E-4 1.8377461293391025E-5 16-17 0.0 0.0 0.0 3.859266871612115E-4 3.675492258678205E-5 18-19 0.0 0.0 0.0 5.329463775083398E-4 3.675492258678205E-5 20-21 0.0 0.0 0.0 0.0012129124453638075 3.675492258678205E-5 22-23 0.0 0.0 0.0 0.004226816097479935 3.675492258678205E-5 24-25 0.0 0.0 0.0 0.012956110211840672 5.513238388017308E-5 26-27 0.0 0.0 0.0 0.027143510330338544 7.35098451735641E-5 28-29 0.0 0.0 0.0 0.06362277099771974 7.35098451735641E-5 30-31 0.0 0.0 0.0 0.1828373624079473 7.35098451735641E-5 32-33 0.0 0.0 0.0 0.43639119587286324 7.35098451735641E-5 34-35 0.0 0.0 0.0 0.8141766676811025 7.35098451735641E-5 36-37 0.0 0.0 0.0 1.3659231880925813 9.188730646695512E-5 38-39 0.0 0.0 0.0 2.106185706451665 1.1026476776034614E-4 40-41 0.0 0.0 0.0 2.867508795452975 1.1026476776034614E-4 42-43 0.0 0.0 0.0 3.5885852442217585 1.1026476776034614E-4 44-45 0.0 0.0 0.0 4.4351797536244035 1.1026476776034614E-4 46-47 0.0 0.0 0.0 5.399978094066139 1.1026476776034614E-4 48-49 0.0 0.0 0.0 6.372053909180057 1.1026476776034614E-4 50-51 0.0 0.0 0.0 7.309892513904387 1.1026476776034614E-4 52-53 0.0 0.0 0.0 8.196660153694385 1.2864222905373718E-4 54-55 0.0 0.0 0.0 9.142511331542632 1.470196903471282E-4 56-57 0.0 0.0 0.0 10.201383896345238 1.470196903471282E-4 58-59 0.0 0.0 0.0 11.311364181004762 1.470196903471282E-4 60-61 0.0 0.0 0.0 12.393906915953252 1.470196903471282E-4 62-63 0.0 0.0 0.0 13.467720356787385 1.470196903471282E-4 64-65 0.0 0.0 0.0 14.507939798377198 1.6539715164051922E-4 66-67 0.0 0.0 0.0 15.62848712328042 1.8377461293391024E-4 68-69 0.0 0.0 0.0 16.81328205286534 1.8377461293391024E-4 70-71 0.0 0.0 0.0 18.00274485761878 1.8377461293391024E-4 72-73 0.0 3.675492258678205E-5 0.0 19.123090030447777 2.205295355206923E-4 74-75 0.0 3.675492258678205E-5 0.0 20.19570893629784 2.205295355206923E-4 76-77 0.0 3.675492258678205E-5 0.0 21.318884238166017 2.205295355206923E-4 78-79 0.0 3.675492258678205E-5 0.0 22.495960634007712 2.205295355206923E-4 80-81 0.0 7.35098451735641E-5 0.0 23.664234225889874 2.205295355206923E-4 82-83 0.0 7.35098451735641E-5 0.0 24.790846113019917 2.940393806942564E-4 84-85 0.0 7.35098451735641E-5 0.0 25.864365514473352 2.940393806942564E-4 86-87 0.0 7.35098451735641E-5 0.0 26.939722662056127 2.940393806942564E-4 88 0.0 7.35098451735641E-5 0.0 27.75312747636291 2.940393806942564E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5250 0.0 50.07429 1 TACCTGG 1960 0.0 46.34737 2 GTATCAA 9390 0.0 40.13879 1 ACCTGGG 2480 0.0 36.775143 3 ATCAACG 11265 0.0 32.926846 3 CAACGCA 11350 0.0 32.887356 5 TATCAAC 11795 0.0 31.64456 2 TCAACGC 11925 0.0 31.143332 4 AACGCAG 12105 0.0 30.913815 6 GTACATG 9395 0.0 28.0822 1 ACGCAGA 13230 0.0 27.929749 7 CGCAGAG 13490 0.0 27.214695 8 GTACTAG 1025 0.0 27.118649 1 TACATGG 9590 0.0 27.043121 2 ACATGGG 9420 0.0 26.550034 3 CATGGGG 6510 0.0 26.06889 4 ATGGGGG 4960 0.0 25.875294 5 CCTGGGG 2550 0.0 25.441063 4 GCAGAGT 14440 0.0 24.642065 9 CGCCTAC 495 0.0 23.743193 7 >>END_MODULE