FastQCFastQC Report
Mon 6 Jun 2016
ERR1211460_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211460_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2720724
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104760.38504456901912876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94830.34854693089045413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52900.19443354048407702No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33990.12492998187247219No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51600.052.6776471
TACCTGG23500.046.801162
ACCTGGG24350.044.7814033
GTATCAA89150.039.0881461
ATCAACG108000.031.8128263
CAACGCA108200.031.7881135
TAACGCA2550.031.334694
TATCAAC112300.030.8458232
TCAACGC114300.030.142164
AACGCAG117200.029.6655986
CCTGGGG23350.029.5901414
GTACATG95250.028.8334141
TACATGG97600.027.6902372
TGGGGGG50200.026.7677156
ACGCAGA128250.026.7066357
ACATGGG97100.026.3322723
GTACCTG42900.026.1992511
CGCAGAG130400.026.158218
GTACACG3100.025.7890591
GTACTAG10650.025.6110081
Produced by FastQC (version 0.11.5)