FastQCFastQC Report
Mon 6 Jun 2016
ERR1211459_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211459_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3868213
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158890.41075814594491045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132780.3432592776044132No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72030.18621001480528607No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41610.10756905061846388No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70100.056.6483231
GTATCAA128650.040.8997731
TACCTGG30200.039.4268072
ATCAACG151400.034.058453
CAACGCA152100.033.8707965
TATCAAC154350.033.631552
TCAACGC158400.032.553344
AACGCAG162000.032.1196756
ACGCAGA179000.028.728577
ACCTGGG40850.028.6517643
CGCAGAG182850.027.9433658
GTACATG128800.027.2469521
ATAACGC3800.027.2134233
GTGGTAT25650.025.8946041
TACATGG131800.025.8167112
GCAGAGT196000.025.1562949
ACATGGG128650.025.1375373
GTACTAG11200.024.3942531
TAACGCA4500.024.024784
CCTGGGG39550.023.5322154
Produced by FastQC (version 0.11.5)