##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211459_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3868213 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.36898691979992 31.0 31.0 34.0 28.0 34.0 2 31.407348561209012 31.0 31.0 34.0 28.0 34.0 3 31.470947696003297 31.0 31.0 34.0 28.0 34.0 4 35.11108178375906 37.0 35.0 37.0 32.0 37.0 5 35.16514680034424 37.0 35.0 37.0 32.0 37.0 6 35.23059407535211 37.0 35.0 37.0 32.0 37.0 7 35.165766724841674 37.0 35.0 37.0 32.0 37.0 8 35.16939113745804 37.0 35.0 37.0 32.0 37.0 9 36.742405343242474 39.0 37.0 39.0 32.0 39.0 10-11 36.66825379057461 39.0 37.0 39.0 32.0 39.0 12-13 36.58394935852809 39.0 37.0 39.0 32.0 39.0 14-15 37.7662543401824 40.0 37.0 41.0 32.0 41.0 16-17 37.75481662979779 40.0 37.0 41.0 32.0 41.0 18-19 37.75594880633513 40.0 37.0 41.0 32.0 41.0 20-21 37.734542927186276 40.0 37.0 41.0 32.0 41.0 22-23 37.60821069056952 40.0 37.0 41.0 32.0 41.0 24-25 37.51404459888843 40.0 37.0 41.0 32.0 41.0 26-27 37.37354987949216 40.0 36.5 41.0 31.0 41.0 28-29 37.23503760004943 40.0 36.0 41.0 31.0 41.0 30-31 37.06679156499396 39.0 36.0 41.0 30.5 41.0 32-33 36.84851015184531 39.0 35.5 41.0 30.0 41.0 34-35 36.716626126844616 39.0 35.0 41.0 30.0 41.0 36-37 36.575679260681866 39.0 35.0 41.0 30.0 41.0 38-39 36.410025895678444 39.0 35.0 40.5 30.0 41.0 40-41 36.236120141264195 39.0 35.0 40.0 29.0 41.0 42-43 36.0257456091482 38.5 35.0 40.0 28.0 41.0 44-45 35.87855451600002 38.0 35.0 40.0 27.5 41.0 46-47 35.64150991685308 38.0 34.5 40.0 26.5 41.0 48-49 35.37760071640316 38.0 34.0 40.0 26.0 41.0 50-51 34.69659232829216 37.5 33.5 39.5 25.0 40.5 52-53 34.98481779571084 38.0 33.5 39.5 26.0 40.5 54-55 35.38390737531775 38.0 34.0 40.0 26.0 41.0 56-57 35.296612285828104 38.0 34.0 40.0 26.0 41.0 58-59 35.086525871248554 38.0 34.0 40.0 26.0 41.0 60-61 34.85351698575027 37.5 34.0 40.0 26.0 41.0 62-63 34.56005356995594 37.0 33.0 40.0 25.0 41.0 64-65 34.34854686130262 37.0 33.0 40.0 25.0 41.0 66-67 34.14089710158153 36.0 33.0 39.5 25.0 41.0 68-69 33.78894220664684 36.0 33.0 39.0 24.0 41.0 70-71 33.40436746890619 35.0 32.5 39.0 23.5 40.5 72-73 33.01243868938965 35.0 32.0 38.0 22.5 40.0 74-75 32.625249178367376 35.0 32.0 37.0 21.5 39.5 76-77 32.23201294758071 35.0 31.0 37.0 20.5 39.0 78-79 31.830004836858777 35.0 31.0 36.0 20.0 39.0 80-81 31.426586514237968 35.0 31.0 36.0 19.0 37.5 82-83 31.0561855047796 34.5 30.0 35.0 18.0 37.0 84-85 30.716835008827076 34.0 30.0 35.0 17.5 37.0 86-87 30.37881031887334 34.0 29.0 35.0 16.0 36.0 88-89 30.057862506537255 34.0 29.0 35.0 14.0 36.0 90-91 29.749395263394234 34.0 29.0 35.0 11.5 35.5 92-93 29.44467742598456 34.0 28.5 35.0 10.0 35.0 94-95 29.147220822638257 34.0 27.0 35.0 10.0 35.0 96-97 28.844344662509535 34.0 26.5 35.0 10.0 35.0 98-99 28.515601390099253 33.5 25.0 35.0 9.0 35.0 100 27.25630879168236 32.0 24.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2967330323791266 1101 2 -0.3133811255368357 1101 3 -0.4029333366013823 1101 4 -0.22350695920130192 1101 5 -0.3271103734971277 1101 6 -0.21873749061237646 1101 7 -0.21350171810049545 1101 8 -0.24857356484570658 1101 9 -0.43382351778564043 1101 10-11 -0.36218777212114617 1101 12-13 -0.4161236987415222 1101 14-15 -0.5730023953582659 1101 16-17 -0.615046735278753 1101 18-19 -0.6548706403175046 1101 20-21 -0.6600586738864536 1101 22-23 -0.5513248832319348 1101 24-25 -0.6563884798943391 1101 26-27 -0.7385810143885578 1101 28-29 -0.7563336269478214 1101 30-31 -0.7013564469896849 1101 32-33 -0.748143309570878 1101 34-35 -0.8502749750828613 1101 36-37 -1.0053269754185408 1101 38-39 -0.9241598331230634 1101 40-41 -1.0902751675177669 1101 42-43 -1.0268227444647096 1101 44-45 -1.052185666598625 1101 46-47 -1.0573480504128199 1101 48-49 -1.105754342417434 1101 50-51 -1.0045232312825405 1101 52-53 -0.8074953287702087 1101 54-55 -0.8041765038457029 1101 56-57 -0.7986346969598088 1101 58-59 -0.7431050660273044 1101 60-61 -0.7682011589312694 1101 62-63 -0.8899155012927551 1101 64-65 -0.8585998463373983 1101 66-67 -0.9281774937717273 1101 68-69 -0.957639381480476 1101 70-71 -0.9116527167628519 1101 72-73 -1.0214025426350446 1101 74-75 -1.2288394426828866 1101 76-77 -1.2561280348569106 1101 78-79 -1.3301888351595856 1101 80-81 -1.460152875323537 1101 82-83 -1.4563402715159377 1101 84-85 -1.6051318189002721 1101 86-87 -1.5485700093142363 1101 88-89 -1.7033865411539253 1101 90-91 -1.7488308627068996 1101 92-93 -1.9379982433961054 1101 94-95 -2.044090070020559 1101 96-97 -2.12080515508649 1101 98-99 -2.236770435998153 1101 100 -2.0890577614443693 1102 1 -0.430000289227749 1102 2 -0.3947979919132578 1102 3 -0.5493053262502734 1102 4 -0.43112152736318166 1102 5 -0.44951824042401256 1102 6 -0.29044541054193473 1102 7 -0.528411711815103 1102 8 -0.4047008750009198 1102 9 -0.6188410809781573 1102 10-11 -0.5199042453136684 1102 12-13 -0.5941527959748285 1102 14-15 -0.7536522819514246 1102 16-17 -0.7418666878619362 1102 18-19 -0.7780832412760574 1102 20-21 -0.8193394200843684 1102 22-23 -0.8451049428579651 1102 24-25 -0.7919602916974071 1102 26-27 -0.8058531376314235 1102 28-29 -0.8495633024682689 1102 30-31 -0.8863125711034385 1102 32-33 -1.0012880341024157 1102 34-35 -1.040217751589502 1102 36-37 -0.8932364197184484 1102 38-39 -1.0160962749855855 1102 40-41 -1.2902365748682953 1102 42-43 -1.111184756616808 1102 44-45 -1.2112747595508395 1102 46-47 -1.3032003885955206 1102 48-49 -1.3097779339756812 1102 50-51 -1.0294819072795605 1102 52-53 -0.8678104260941666 1102 54-55 -0.9991854498471824 1102 56-57 -0.9278288965867674 1102 58-59 -0.8923012882968564 1102 60-61 -1.0598062227689624 1102 62-63 -0.9041801095799826 1102 64-65 -1.0085062205173685 1102 66-67 -0.9779327985580224 1102 68-69 -1.1082642915816052 1102 70-71 -1.0749584431207921 1102 72-73 -1.0685872311258144 1102 74-75 -1.2689140970585946 1102 76-77 -1.3026585085089764 1102 78-79 -1.4713408611164844 1102 80-81 -1.5012743520412748 1102 82-83 -1.4589715408892268 1102 84-85 -1.4236437932326247 1102 86-87 -1.5386418995863984 1102 88-89 -1.5621876171369031 1102 90-91 -1.7530856171614921 1102 92-93 -1.788256274426903 1102 94-95 -1.8147533160108402 1102 96-97 -1.92967892306266 1102 98-99 -1.8526390365416994 1102 100 -1.8957905936758586 1103 1 -0.4475741307922547 1103 2 -0.5045675319593244 1103 3 -0.543218705563973 1103 4 -0.38513963973529997 1103 5 -0.4058494135530495 1103 6 -0.36835846643319314 1103 7 -0.4828544055805679 1103 8 -0.6405010557454034 1103 9 -0.6052171790581582 1103 10-11 -0.6038574120272031 1103 12-13 -0.7705626083397235 1103 14-15 -0.8534366014012065 1103 16-17 -0.9014753932205153 1103 18-19 -0.9404994877439989 1103 20-21 -0.8459623817895832 1103 22-23 -0.8894584933136613 1103 24-25 -0.9051814450275444 1103 26-27 -0.9977074000457975 1103 28-29 -0.9544670163166913 1103 30-31 -1.0023209621984464 1103 32-33 -0.941664803355529 1103 34-35 -0.9971908502841487 1103 36-37 -1.0038315545720096 1103 38-39 -1.0300880073206002 1103 40-41 -1.0818951470297762 1103 42-43 -1.0686795707669674 1103 44-45 -0.9583014376088457 1103 46-47 -1.080391978109482 1103 48-49 -0.9252987764768221 1103 50-51 -0.9895086915947218 1103 52-53 -0.810122587064086 1103 54-55 -0.9210714623991194 1103 56-57 -0.8903306764834937 1103 58-59 -0.8166631908508251 1103 60-61 -0.9095207774227063 1103 62-63 -0.8778497128079081 1103 64-65 -0.8997979997915735 1103 66-67 -0.8898236177255328 1103 68-69 -0.8600915925855759 1103 70-71 -0.9895740247838845 1103 72-73 -0.9298072975562093 1103 74-75 -1.028330197914876 1103 76-77 -1.1566402984661366 1103 78-79 -1.0422290045357308 1103 80-81 -1.1551570670438487 1103 82-83 -1.0478453617747299 1103 84-85 -1.2516686516816407 1103 86-87 -1.5042385084448817 1103 88-89 -1.4438249772227145 1103 90-91 -1.3382949974139784 1103 92-93 -1.452813092665636 1103 94-95 -1.478033035992837 1103 96-97 -1.81753724578817 1103 98-99 -1.8714627859808992 1103 100 -1.9397519854799619 1104 1 -0.5166682351570238 1104 2 -0.3862967637457082 1104 3 -0.44252185603332705 1104 4 -0.27662474744153087 1104 5 -0.2857897281167254 1104 6 -0.24414322976804215 1104 7 -0.3636742083706963 1104 8 -0.4315643838002927 1104 9 -0.4737126781375238 1104 10-11 -0.5332224085708788 1104 12-13 -0.5737392102059218 1104 14-15 -0.7319144581883776 1104 16-17 -0.6846461767722118 1104 18-19 -0.739939796704725 1104 20-21 -0.7877277596140786 1104 22-23 -0.7467560131673636 1104 24-25 -0.7133828434540987 1104 26-27 -0.6453136442546281 1104 28-29 -0.7990113878371687 1104 30-31 -0.8951374711537099 1104 32-33 -0.8515146337612052 1104 34-35 -0.9312182900366608 1104 36-37 -1.0536248494504221 1104 38-39 -1.0736071350952017 1104 40-41 -0.952815804690168 1104 42-43 -0.975826239917609 1104 44-45 -0.922223948095116 1104 46-47 -1.013910993825057 1104 48-49 -0.9675149117152486 1104 50-51 -0.9191857462494042 1104 52-53 -0.7509756906437417 1104 54-55 -0.7663622958310796 1104 56-57 -0.6685856583824545 1104 58-59 -0.7773250464501658 1104 60-61 -0.7991770468798265 1104 62-63 -0.8512268530476774 1104 64-65 -0.7729562922642188 1104 66-67 -0.7555732330479188 1104 68-69 -0.69301929560676 1104 70-71 -0.8824702882458482 1104 72-73 -0.7591620249411264 1104 74-75 -0.893005797553208 1104 76-77 -0.9266455039578716 1104 78-79 -0.8923890650896276 1104 80-81 -0.9794175300297034 1104 82-83 -0.9892072395757694 1104 84-85 -1.0419726923198454 1104 86-87 -1.1424329767213273 1104 88-89 -1.2156312688465114 1104 90-91 -1.2251960156803001 1104 92-93 -1.1233468753089575 1104 94-95 -1.286075450682656 1104 96-97 -1.2935004543601245 1104 98-99 -1.2888143112792712 1104 100 -1.3609467412578304 1105 1 -0.41037213331895117 1105 2 -0.4561204937746375 1105 3 -0.4083818374389736 1105 4 -0.3368115860670855 1105 5 -0.38352781350723575 1105 6 -0.33248808291021703 1105 7 -0.31867131154316297 1105 8 -0.30523725755710984 1105 9 -0.4545481246699268 1105 10-11 -0.4865140612975125 1105 12-13 -0.5900645372332534 1105 14-15 -0.7681288932996395 1105 16-17 -0.6799786884718131 1105 18-19 -0.5720095470424553 1105 20-21 -0.6855593813195782 1105 22-23 -0.6227891815766711 1105 24-25 -0.6429435426887835 1105 26-27 -0.6877701065446047 1105 28-29 -0.7252164144977229 1105 30-31 -0.7027814139450257 1105 32-33 -0.8660287992519144 1105 34-35 -0.7546104034820118 1105 36-37 -0.752299639834412 1105 38-39 -0.9188411072033418 1105 40-41 -0.7991428474772277 1105 42-43 -0.6701097244988006 1105 44-45 -0.6046819862330892 1105 46-47 -0.6551755799305425 1105 48-49 -0.812064625423929 1105 50-51 -0.8017914117256737 1105 52-53 -0.6506289059013852 1105 54-55 -0.469340722177499 1105 56-57 -0.5380965261289603 1105 58-59 -0.49339945691242093 1105 60-61 -0.5462350344041198 1105 62-63 -0.547871777757571 1105 64-65 -0.6293342342869224 1105 66-67 -0.6555630955834104 1105 68-69 -0.6605848501878384 1105 70-71 -0.6163057165324517 1105 72-73 -0.45737543386934476 1105 74-75 -0.600989668593531 1105 76-77 -0.5219836530368553 1105 78-79 -0.6439280737503275 1105 80-81 -0.5868777629819313 1105 82-83 -0.62484475559096 1105 84-85 -0.7203167117189757 1105 86-87 -0.6896956935732668 1105 88-89 -0.6497935914991793 1105 90-91 -0.6951698930951977 1105 92-93 -0.702255009016465 1105 94-95 -0.8987655598473552 1105 96-97 -1.0472688051959231 1105 98-99 -0.9751977736791382 1105 100 -0.9019982769691559 1106 1 -0.48716752458429013 1106 2 -0.5238284141867702 1106 3 -0.5507661660953715 1106 4 -0.4109178366753454 1106 5 -0.43187126266818154 1106 6 -0.4997150960073853 1106 7 -0.49652568002197484 1106 8 -0.4133017519912343 1106 9 -0.6038146974971568 1106 10-11 -0.7677491417375322 1106 12-13 -0.7062722135520829 1106 14-15 -0.8892585507540645 1106 16-17 -0.9235502125555612 1106 18-19 -0.9778917999167831 1106 20-21 -0.9543125907310568 1106 22-23 -0.9078796306054784 1106 24-25 -0.9646335070123939 1106 26-27 -0.9598751085978421 1106 28-29 -0.8643607507815432 1106 30-31 -0.8950491554134388 1106 32-33 -1.0474669083477792 1106 34-35 -0.9089689524047131 1106 36-37 -0.9801675778434173 1106 38-39 -1.1373891912094223 1106 40-41 -1.0073151464879544 1106 42-43 -0.8775766848686573 1106 44-45 -1.0283454270703345 1106 46-47 -0.876182755561409 1106 48-49 -0.8879984899865647 1106 50-51 -1.0072408638410622 1106 52-53 -0.9831397595474698 1106 54-55 -0.8095802738370494 1106 56-57 -0.9251991821963585 1106 58-59 -0.959879196377436 1106 60-61 -0.8439014708239441 1106 62-63 -0.9028476809621182 1106 64-65 -0.8393502487062321 1106 66-67 -0.8700019460582595 1106 68-69 -0.8087725860227124 1106 70-71 -0.8992232604712243 1106 72-73 -0.7312388175800564 1106 74-75 -0.8130066941999843 1106 76-77 -0.8347254023273223 1106 78-79 -0.8751710664528467 1106 80-81 -0.8684196018392107 1106 82-83 -0.859558106188846 1106 84-85 -0.9770282883876149 1106 86-87 -0.8806613931240896 1106 88-89 -0.9147523963044542 1106 90-91 -0.9944909418497687 1106 92-93 -1.1328795001590848 1106 94-95 -1.1965003977765285 1106 96-97 -1.193755760644347 1106 98-99 -1.31548721215378 1106 100 -1.3745021361751917 1107 1 -0.584454263699282 1107 2 -0.5648352672211452 1107 3 -0.583374023294418 1107 4 -0.4577419405989005 1107 5 -0.39504313383754663 1107 6 -0.4226424025197204 1107 7 -0.4911211539490594 1107 8 -0.3953114866146734 1107 9 -0.5566756274110745 1107 10-11 -0.5707044698514636 1107 12-13 -0.7409593896788138 1107 14-15 -0.7796948903951204 1107 16-17 -0.864233736480081 1107 18-19 -0.8620617334790879 1107 20-21 -0.8146082953838345 1107 22-23 -0.7263239391042973 1107 24-25 -0.8153214453511168 1107 26-27 -0.8636773092367847 1107 28-29 -0.8157623272925747 1107 30-31 -0.7953678638994219 1107 32-33 -0.8958685543591116 1107 34-35 -0.808406801958732 1107 36-37 -0.8025142896012625 1107 38-39 -0.6914544619130538 1107 40-41 -0.8078688194256856 1107 42-43 -0.7713696977216244 1107 44-45 -0.8871216720229711 1107 46-47 -0.7658865926649412 1107 48-49 -0.8680255951007965 1107 50-51 -0.9384670017556118 1107 52-53 -0.7450833085073754 1107 54-55 -0.6298124200901114 1107 56-57 -0.6698656838953028 1107 58-59 -0.6900299127268923 1107 60-61 -0.7215702629776004 1107 62-63 -0.7351454599313243 1107 64-65 -0.7106835545449428 1107 66-67 -0.7321261194057413 1107 68-69 -0.5938407154032319 1107 70-71 -0.536467130889072 1107 72-73 -0.5091355477822006 1107 74-75 -0.5333865094819537 1107 76-77 -0.5726869969237143 1107 78-79 -0.651605426481165 1107 80-81 -0.705573732867883 1107 82-83 -0.7409002179437927 1107 84-85 -0.8327642010219272 1107 86-87 -0.7870836784311912 1107 88-89 -0.7621521306758616 1107 90-91 -0.80315251131913 1107 92-93 -0.8862088314975303 1107 94-95 -0.9454583516268045 1107 96-97 -0.9531709118609442 1107 98-99 -0.9140383601687851 1107 100 -0.7301806537874072 1108 1 -0.5365321189241818 1108 2 -0.5296313099231256 1108 3 -0.5507090509798545 1108 4 -0.4123885508998626 1108 5 -0.4367745953351658 1108 6 -0.46097962466489406 1108 7 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50-51 1.487493518207522 2311 52-53 1.3767422288659503 2311 54-55 1.2400432189336428 2311 56-57 1.2680455910723651 2311 58-59 1.2028539384520442 2311 60-61 1.2413768386488115 2311 62-63 1.2530426135511306 2311 64-65 1.2188950906719285 2311 66-67 1.2633471751087129 2311 68-69 1.3566840686677324 2311 70-71 1.3138798016769258 2311 72-73 1.2796104769764653 2311 74-75 1.3618388112764563 2311 76-77 1.3664721414098153 2311 78-79 1.3322161577318248 2311 80-81 1.3225511898781583 2311 82-83 1.3801116712145536 2311 84-85 1.3632913589291746 2311 86-87 1.45455559276704 2311 88-89 1.5363116226694018 2311 90-91 1.523799490663766 2311 92-93 1.4808213557628065 2311 94-95 1.6557781482173475 2311 96-97 1.7424374528002495 2311 98-99 1.7622302168439425 2311 100 1.7979292237974924 2312 1 0.5496617267385453 2312 2 0.5939155659827833 2312 3 0.6807798799028717 2312 4 0.542001363927497 2312 5 0.5642632390765385 2312 6 0.4908617864757616 2312 7 0.6289621505984684 2312 8 0.6592917604246153 2312 9 0.8598502792890983 2312 10-11 0.9448063277320102 2312 12-13 0.9201933420152599 2312 14-15 1.0836646829199594 2312 16-17 1.1006121881359618 2312 18-19 1.0319850970712636 2312 20-21 1.0928944485111955 2312 22-23 1.1534570140915648 2312 24-25 1.1443512203838395 2312 26-27 1.197469031479443 2312 28-29 1.2803933674912287 2312 30-31 1.1275815037106796 2312 32-33 1.2286554234277531 2312 34-35 1.1939026650337112 2312 36-37 1.1872020460550061 2312 38-39 1.1909285699879604 2312 40-41 1.2189677416155504 2312 42-43 1.2869860040341479 2312 44-45 1.3091360143162873 2312 46-47 1.1925332301412368 2312 48-49 1.1646341502953206 2312 50-51 1.0992595742542477 2312 52-53 0.9696205356922007 2312 54-55 0.9723806360332432 2312 56-57 1.0012704418199903 2312 58-59 0.9477569286194623 2312 60-61 0.8571412313809148 2312 62-63 0.919072532625556 2312 64-65 0.9728341664217979 2312 66-67 0.9940550759078235 2312 68-69 0.9869288322739891 2312 70-71 0.9787790485209982 2312 72-73 0.9799974130785998 2312 74-75 1.0653828839667128 2312 76-77 1.0269074136643894 2312 78-79 1.0755196264688784 2312 80-81 0.97504269543116 2312 82-83 1.160518156592861 2312 84-85 1.2288538044225525 2312 86-87 1.28355907435839 2312 88-89 1.329137441889202 2312 90-91 1.4745399653907718 2312 92-93 1.6243935894682586 2312 94-95 1.542513669622931 2312 96-97 1.6941709695372928 2312 98-99 1.5353044254735941 2312 100 1.7706781668711926 2313 1 0.6011113953488056 2313 2 0.6034526268216709 2313 3 0.6844460446905458 2313 4 0.5479161913912947 2313 5 0.5318893242708356 2313 6 0.592031885369039 2313 7 0.5327345396376444 2313 8 0.6069576151082074 2313 9 0.7605046261187596 2313 10-11 0.6740025596952961 2313 12-13 0.7820098039180223 2313 14-15 1.0147046741064756 2313 16-17 1.0102508488671234 2313 18-19 0.9641552000448641 2313 20-21 0.9770551341132574 2313 22-23 0.9959380100153297 2313 24-25 1.1024216792970876 2313 26-27 1.0871112828895377 2313 28-29 1.0410283736185022 2313 30-31 1.0960025026538815 2313 32-33 1.080494558291754 2313 34-35 1.135624517987253 2313 36-37 1.1358581936645535 2313 38-39 1.1793083033256408 2313 40-41 1.2555945027823512 2313 42-43 1.3715534972370165 2313 44-45 1.3108782416525102 2313 46-47 1.2414447375871376 2313 48-49 1.307068018558212 2313 50-51 1.2622001634792213 2313 52-53 1.2039160757697545 2313 54-55 1.1427223761796128 2313 56-57 1.1104412262065182 2313 58-59 1.0750770988502865 2313 60-61 1.1618918084057341 2313 62-63 1.1812870294476738 2313 64-65 1.0920335538302126 2313 66-67 1.1474327622865061 2313 68-69 1.0969445822974535 2313 70-71 1.1249624560480882 2313 72-73 1.2058016775538647 2313 74-75 1.2299666168310779 2313 76-77 1.1406229230851679 2313 78-79 1.2813439389014682 2313 80-81 1.278414125323831 2313 82-83 1.273033440780143 2313 84-85 1.3401269041169357 2313 86-87 1.3618483198669828 2313 88-89 1.4833940965461387 2313 90-91 1.592380811267681 2313 92-93 1.5907983677914395 2313 94-95 1.657549705622209 2313 96-97 1.6583128040750026 2313 98-99 1.7732449806959245 2313 100 1.8474750364520212 2314 1 0.4167736619142772 2314 2 0.47811919685655724 2314 3 0.46245063872635583 2314 4 0.3865356185393196 2314 5 0.4350689795235425 2314 6 0.42703782084182507 2314 7 0.5011657428442078 2314 8 0.5105593633381318 2314 9 0.7174400029457857 2314 10-11 0.6601807258791439 2314 12-13 0.3709822390321378 2314 14-15 0.747519349789421 2314 16-17 0.7822287067687554 2314 18-19 0.7772463231410001 2314 20-21 0.7637991330449339 2314 22-23 0.714386694179062 2314 24-25 0.6597982070354362 2314 26-27 0.781951749862607 2314 28-29 0.8179257285248767 2314 30-31 0.8672436774547307 2314 32-33 0.9196700207989537 2314 34-35 1.0513767057911139 2314 36-37 0.9762688653023872 2314 38-39 1.0972928194252347 2314 40-41 1.1313358329322085 2314 42-43 1.195842968466593 2314 44-45 1.180008750251055 2314 46-47 1.2781391791795969 2314 48-49 1.2750590103290804 2314 50-51 1.1434462168427757 2314 52-53 1.0095556808410464 2314 54-55 1.0341651624495825 2314 56-57 0.9879972877368033 2314 58-59 0.9817556297519943 2314 60-61 0.9514736947788478 2314 62-63 0.8887137184738592 2314 64-65 0.8662465584544421 2314 66-67 0.9698431480553253 2314 68-69 0.9310576132941009 2314 70-71 0.9658714523995684 2314 72-73 0.9972896928646335 2314 74-75 1.0820530915360393 2314 76-77 1.0881801186967408 2314 78-79 1.1937199868361965 2314 80-81 1.2307859993025723 2314 82-83 1.1524432890346965 2314 84-85 1.154610732721686 2314 86-87 1.4163193850243125 2314 88-89 1.5061097447876755 2314 90-91 1.645718667505097 2314 92-93 1.7008104937375386 2314 94-95 1.75980458133294 2314 96-97 1.806423820567499 2314 98-99 1.9108034714417563 2314 100 1.7837453047998117 2315 1 0.4230335847311295 2315 2 0.44266319659353215 2315 3 0.5002836738366057 2315 4 0.45375139395491004 2315 5 0.3941078390276189 2315 6 -0.2027106089739732 2315 7 0.31704510454738255 2315 8 0.41039525115534303 2315 9 0.6145687932038584 2315 10-11 0.72170533897242 2315 12-13 0.7434589374398826 2315 14-15 0.8600303660577708 2315 16-17 0.801471773849876 2315 18-19 0.8521250199019264 2315 20-21 0.8825786451875928 2315 22-23 0.7991085203392814 2315 24-25 0.8617757064716756 2315 26-27 0.9024714600950361 2315 28-29 1.0556599039092163 2315 30-31 1.018842289733037 2315 32-33 1.1124368526825847 2315 34-35 1.075196901731438 2315 36-37 1.071258872166574 2315 38-39 1.0090534987260256 2315 40-41 1.0369461831402802 2315 42-43 1.1319301843896028 2315 44-45 1.1768415579101372 2315 46-47 1.1538787655773106 2315 48-49 1.1991396813855886 2315 50-51 1.0624874547074228 2315 52-53 1.0261837002530996 2315 54-55 0.7579198849779303 2315 56-57 0.7521207259765177 2315 58-59 0.7738140884585576 2315 60-61 0.692808686866222 2315 62-63 0.818901521903193 2315 64-65 0.8725282674430659 2315 66-67 1.0115210226688802 2315 68-69 1.0794797089267405 2315 70-71 1.0871870428624888 2315 72-73 1.126409770598407 2315 74-75 1.1594193227841885 2315 76-77 1.1614058062460089 2315 78-79 1.0623712235019447 2315 80-81 1.1908651595565694 2315 82-83 1.3619166235779723 2315 84-85 1.3063840541888538 2315 86-87 1.4511907160263924 2315 88-89 1.6355807654892622 2315 90-91 1.7578731285463611 2315 92-93 1.8649171968388174 2315 94-95 2.016482363366311 2315 96-97 2.0581498443883106 2315 98-99 2.220191172630752 2315 100 2.1908428595207745 2316 1 0.09268630406628375 2316 2 -0.03044828323738713 2316 3 0.15149767982325457 2316 4 0.07473090689410355 2316 5 0.15422345978388563 2316 6 0.20436751632487926 2316 7 0.1895266592592364 2316 8 0.2851729035471351 2316 9 0.4029646986607034 2316 10-11 0.34702825105665625 2316 12-13 0.39317045337239875 2316 14-15 0.5870782054606352 2316 16-17 0.6400258927795548 2316 18-19 0.6132969412243483 2316 20-21 0.5629352799924092 2316 22-23 0.4175869215404404 2316 24-25 0.5047824259120262 2316 26-27 0.6454069924382395 2316 28-29 0.7236623497329049 2316 30-31 0.6683407225410178 2316 32-33 0.840533820148103 2316 34-35 0.8477620459943935 2316 36-37 0.8620639916604844 2316 38-39 0.8910871843886667 2316 40-41 0.9366417103671054 2316 42-43 1.0211102237905507 2316 44-45 0.8999880036955759 2316 46-47 0.9061025405137073 2316 48-49 0.9191855136828977 2316 50-51 0.7165894393391383 2316 52-53 0.5638752060962986 2316 54-55 0.5013289519786852 2316 56-57 0.6000548357732001 2316 58-59 0.7004499870776115 2316 60-61 0.754502527359314 2316 62-63 0.7797445839405768 2316 64-65 0.7817520172757142 2316 66-67 0.7472413153548345 2316 68-69 0.7527224156861081 2316 70-71 0.6923103919912776 2316 72-73 0.8361774053936486 2316 74-75 0.9393855940865166 2316 76-77 0.9388938281651775 2316 78-79 1.0033210482537775 2316 80-81 1.1849562120975499 2316 82-83 1.2559165853246306 2316 84-85 1.2866692329276361 2316 86-87 1.4648265417249924 2316 88-89 1.570137947077928 2316 90-91 1.6223391117531385 2316 92-93 1.7260778513536685 2316 94-95 1.8132683047813032 2316 96-97 1.9711893660779616 2316 98-99 2.0532218661640265 2316 100 2.0952791127005845 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 21.0 1 239.0 2 120.0 3 74.0 4 64.0 5 47.0 6 92.0 7 450.0 8 1495.0 9 2637.0 10 3472.0 11 4231.0 12 5276.0 13 6138.0 14 7465.0 15 9624.0 16 12882.0 17 17266.0 18 21634.0 19 27088.0 20 32810.0 21 38822.0 22 42628.0 23 43202.0 24 43732.0 25 45814.0 26 50440.0 27 59068.0 28 69562.0 29 82120.0 30 98823.0 31 121363.0 32 151812.0 33 194018.0 34 263062.0 35 357251.0 36 510685.0 37 719066.0 38 655486.0 39 167982.0 40 152.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.36416248471035 18.811509994333665 12.330848730074875 23.493478790881113 2 16.528118119012206 20.881258341326767 39.48567589503811 23.10494764462291 3 19.13705258236746 26.666328791323362 30.202151162670425 23.994467463638752 4 12.94480382049625 17.155101773798137 38.10605760894214 31.79403679676347 5 12.982167339768006 37.31440581675404 34.650651871366534 15.052774972111418 6 29.393030167430144 36.885509649283904 19.070533795126345 14.650926388159613 7 26.25147963280273 33.13826495981193 22.98193088976632 17.628324517619014 8 25.76455403493434 34.30461109487314 21.270269081841956 18.660565788350564 9 27.062290335119837 15.765195507045359 20.934172695454674 36.23834146238013 10-11 24.587706728555325 25.729342468350364 29.122593261345138 20.560357541749173 12-13 25.277943335056218 24.18429975607583 28.439931458640157 22.09782545022779 14-15 22.948210416354225 25.526183100703836 26.597916418867552 24.927690064074387 16-17 22.0149786789963 27.97321115402074 27.365184713726592 22.646625453256366 18-19 21.537092322657415 27.33255280341765 29.535123587638186 21.595231286286754 20-21 22.504946208088132 26.779119018378573 29.207211696154722 21.508723077378566 22-23 22.471896166278555 26.55265544008793 28.990749560116996 21.984698833516518 24-25 22.04801544421112 26.47254352411309 29.332184222795053 22.14725680888073 26-27 21.92827972597908 26.55914426124555 29.193262626821554 22.31931338595382 28-29 21.825732042038197 27.33130614994176 28.98007335611329 21.862888451906752 30-31 22.613775665474943 26.770814021767546 29.018919452121665 21.596490860635846 32-33 22.337737763803283 26.8644184707584 28.76190023051535 22.035943534922968 34-35 22.481899048407115 26.860105502689287 28.96397910633016 21.694016342573438 36-37 22.299216572857276 26.57797979385293 28.96915603095645 22.153647602333347 38-39 22.16752156731124 26.680980660722657 29.046164117651774 22.10533365431433 40-41 22.09777438059836 26.164748922396097 29.524646710507348 22.212829986498196 42-43 21.888856291448867 26.504856364890085 28.882851825757616 22.72343551790343 44-45 21.388744134202465 26.85641096595413 29.270423449483346 22.484421450360063 46-47 21.411343681336906 26.331531392195767 29.076742570791485 23.180382355675842 48-49 21.58211655352872 26.44457619984521 28.97034016385228 23.002967082773786 50-51 21.507049831028773 26.65631173507339 28.904805009776762 22.931833424121077 52-53 21.306522149008746 26.927694530749097 28.56681195850169 23.198971361740462 54-55 21.09052208788601 26.620837608793902 28.983426407442824 23.305213895877262 56-57 21.477043043081878 26.561105399621106 28.547656457108868 23.414195100188156 58-59 21.251768793046235 26.54209914966026 28.683156693273244 23.52297536402026 60-61 21.49103039000834 26.494411367324116 28.561190517748514 23.45336772491903 62-63 21.61827886573059 26.297907530209702 28.45409305729904 23.629720546760666 64-65 21.349513556369303 26.18962962120156 28.330185881123278 24.13067094130586 66-67 21.595504566135652 26.08813259869675 28.0039726440452 24.3123901911224 68-69 21.393599529117008 26.150040162636696 28.173171565577338 24.283188742668962 70-71 21.629695569054554 25.95491570941472 28.081215034096928 24.33417368743379 72-73 21.860707210212958 26.06882005762377 27.871489683069267 24.198983049094004 74-75 22.082045193989874 26.082654424478243 27.750022076847596 24.085278304684287 76-77 22.113119028752745 25.914497175715358 27.764312796619055 24.20807099891285 78-79 22.21329158674236 26.080880184586196 27.72949388119313 23.976334347478314 80-81 22.478257997128 25.92371271065666 27.572674924104838 24.025354368110495 82-83 22.735572849343992 25.607182372299004 27.884027384714276 23.773217393642724 84-85 22.966616473391532 25.81371021036705 27.400434778561817 23.819238537679603 86-87 22.80375747984142 25.83096098129986 27.606304662617088 23.75897687624163 88-89 23.182517728790998 25.205601227479278 27.555929791246815 24.05595125248291 90-91 23.824693973750303 25.01927006717955 27.379281206454888 23.77675475261526 92-93 24.063479824440286 24.996560998830738 27.22260318425651 23.717355992472466 94-95 23.811354194625753 24.727752841936066 27.358557406824165 24.102335556614015 96-97 24.058920163331248 24.52103351414306 27.240070613795446 24.179975708730247 98-99 24.179739350556357 24.61565494786313 26.97745273960683 24.22715296197369 100 24.817636222018333 24.333513662801824 26.814897144967308 24.03395297021254 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2066.0 1 1410.5 2 1856.5 3 2807.0 4 1943.5 5 1318.0 6 1744.5 7 2688.5 8 4076.5 9 4847.0 10 4119.5 11 3304.0 12 3392.5 13 3623.0 14 3940.0 15 4357.0 16 4772.0 17 5145.5 18 5548.0 19 6061.0 20 6518.5 21 7171.0 22 8192.5 23 9574.0 24 11215.5 25 13318.0 26 15999.0 27 19266.0 28 23532.0 29 29360.5 30 36548.0 31 43959.0 32 53131.0 33 61636.5 34 69049.0 35 76657.5 36 84482.0 37 94927.5 38 102218.5 39 104677.0 40 105703.5 41 107826.5 42 112575.5 43 118882.5 44 126684.0 45 136198.5 46 145324.5 47 153230.0 48 160511.5 49 165744.5 50 167335.5 51 168376.5 52 166561.5 53 160347.0 54 151726.5 55 137371.0 56 122113.5 57 106666.0 58 90604.5 59 76250.5 60 61172.5 61 47623.0 62 37191.0 63 28200.0 64 20128.5 65 13856.5 66 9986.0 67 7579.0 68 5747.0 69 4256.0 70 3203.5 71 2488.0 72 1944.5 73 1468.5 74 1081.5 75 753.0 76 564.0 77 407.5 78 276.5 79 190.0 80 129.5 81 99.5 82 82.5 83 67.5 84 50.0 85 36.5 86 35.0 87 34.0 88 25.0 89 17.5 90 14.0 91 11.0 92 9.0 93 7.5 94 9.0 95 6.0 96 3.0 97 3.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22170444078441387 2 0.14652760848484817 3 0.023499223026239763 4 0.02228419169264981 5 0.026859947991488577 6 0.05488322385556328 7 0.061759784169072386 8 0.054366189245525 9 0.04203491379611205 10-11 0.04393501598800273 12-13 0.042461467349393635 14-15 0.021534491508094306 16-17 0.020228979117747653 18-19 0.01951805652894502 20-21 0.022142007174889282 22-23 0.025631990792647663 24-25 0.022594412458672778 26-27 0.019931684216975644 28-29 0.02011264633048904 30-31 0.01840643211736272 32-33 0.0260714702111802 34-35 0.026187802998438814 36-37 0.025179585508864167 38-39 0.023318260912726367 40-41 0.02462377330307302 42-43 0.030362857474497917 44-45 0.03924292690190535 46-47 0.044839826555569715 48-49 0.04189272927835153 50-51 0.029328788254421357 52-53 0.04503371453433407 54-55 0.04318531580344722 56-57 0.044930307612326414 58-59 0.04849784642159054 60-61 0.025541509735890967 62-63 0.026795318665233794 64-65 0.027506241254036425 66-67 0.03275414254592495 68-69 0.03709723327024649 70-71 0.04107839976754124 72-73 0.045318083569855123 74-75 0.026872873856739532 76-77 0.019996313543230428 78-79 0.019427575472188322 80-81 0.02252978313241799 82-83 0.020681384401531146 84-85 0.012848310059451225 86-87 0.014670857059836156 88-89 0.0200350911389833 90-91 0.021095012089561768 92-93 0.020965753437052197 94-95 0.024688402629327803 96-97 0.02533469589187565 98-99 0.03050504199225844 100 0.03146155602082926 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3868213.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.13596669162222 #Duplication Level Percentage of deduplicated Percentage of total 1 80.85784704215054 53.476118787359525 2 12.175796980680968 16.10516207116542 3 3.168921565064035 6.287390732263153 4 1.306268846768528 3.455654117607486 5 0.6540068184437315 2.1626686580344225 6 0.4216586076104631 1.6732079776896835 7 0.2923329892935494 1.3533607389946376 8 0.20092574028317883 1.0630734453486281 9 0.1453040902985286 0.864884382852596 >10 0.728789071567636 8.312791448529918 >50 0.0328507915413842 1.4814606441994722 >100 0.013859431366265938 1.71096585265126 >500 9.88642143941317E-4 0.4207214918021161 >1k 3.1456795094793305E-4 0.5516099750931988 >5k 4.4938278706847574E-5 0.21407578936402538 >10k+ 8.987655741369515E-5 0.8668538870443604 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15889 0.41075814594491045 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13278 0.3432592776044132 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7203 0.18621001480528607 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4161 0.10756905061846388 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 2.5851730501913934E-5 0.0 2.5851730501913934E-5 0.0 5 0.0 2.5851730501913934E-5 0.0 2.5851730501913934E-5 0.0 6 0.0 2.5851730501913934E-5 0.0 2.5851730501913934E-5 0.0 7 0.0 2.5851730501913934E-5 0.0 2.5851730501913934E-5 0.0 8 0.0 2.5851730501913934E-5 0.0 2.5851730501913934E-5 0.0 9 0.0 2.5851730501913934E-5 0.0 5.170346100382787E-5 0.0 10-11 0.0 2.5851730501913934E-5 0.0 6.462932625478483E-5 6.462932625478483E-5 12-13 0.0 2.5851730501913934E-5 0.0 1.4218451776052662E-4 1.4218451776052662E-4 14-15 0.0 2.5851730501913934E-5 0.0 2.0681384401531144E-4 2.0681384401531144E-4 16-17 0.0 2.5851730501913934E-5 0.0 3.360724965248811E-4 2.5851730501913933E-4 18-19 0.0 2.5851730501913934E-5 0.0 5.687380710421065E-4 2.8436903552105325E-4 20-21 0.0 2.5851730501913934E-5 0.0 0.0012925865250956966 3.2314663127392415E-4 22-23 0.0 2.5851730501913934E-5 0.0 0.005364234079147141 3.3607249652488114E-4 24-25 0.0 2.5851730501913934E-5 0.0 0.013701417166014383 3.489983617758381E-4 26-27 0.0 2.5851730501913934E-5 0.0 0.02688579972199049 3.6192422702679505E-4 28-29 0.0 2.5851730501913934E-5 0.0 0.06518513846057598 3.74850092277752E-4 30-31 0.0 2.5851730501913934E-5 0.0 0.17417603425664513 3.8777595752870897E-4 32-33 0.0 3.87775957528709E-5 0.0 0.403390402751865 4.1362768803062294E-4 34-35 0.0 5.170346100382787E-5 0.0 0.7378859437161294 4.1362768803062294E-4 36-37 0.0 5.170346100382787E-5 0.0 1.2250876567551994 4.1362768803062294E-4 38-39 0.0 5.170346100382787E-5 0.0 1.8876675095192534 4.3947941853253686E-4 40-41 0.0 5.170346100382787E-5 0.0 2.5813340682118593 4.3947941853253686E-4 42-43 0.0 5.170346100382787E-5 0.0 3.2309234263987014 4.524052837834938E-4 44-45 0.0 5.170346100382787E-5 0.0 4.01566563164955 4.7825701428540776E-4 46-47 0.0 5.170346100382787E-5 0.0 4.900389404616551 5.558122057911496E-4 48-49 0.0 5.170346100382787E-5 0.0 5.772510458963868 5.945898015440205E-4 50-51 0.0 5.170346100382787E-5 0.0 6.63456226428069 5.945898015440205E-4 52-53 1.2925865250956967E-5 5.170346100382787E-5 0.0 7.461235976405643 6.075156667949774E-4 54-55 2.5851730501913934E-5 5.170346100382787E-5 0.0 8.352668273437889 6.462932625478483E-4 56-57 2.5851730501913934E-5 5.170346100382787E-5 0.0 9.335499363659654 6.462932625478483E-4 58-59 2.5851730501913934E-5 5.170346100382787E-5 0.0 10.37847450489412 6.462932625478483E-4 60-61 2.5851730501913934E-5 5.170346100382787E-5 0.0 11.396438096867985 6.721449930497623E-4 62-63 2.5851730501913934E-5 5.170346100382787E-5 0.0 12.394806077121398 7.238484540535901E-4 64-65 2.5851730501913934E-5 5.170346100382787E-5 0.0 13.379588972996057 7.497001845555041E-4 66-67 2.5851730501913934E-5 5.170346100382787E-5 0.0 14.446502816675297 7.497001845555041E-4 68-69 2.5851730501913934E-5 5.170346100382787E-5 0.0 15.584715733078815 7.497001845555041E-4 70-71 2.5851730501913934E-5 5.170346100382787E-5 0.0 16.734717038591207 7.75551915057418E-4 72-73 2.5851730501913934E-5 5.170346100382787E-5 0.0 17.81725825335885 8.272553760612459E-4 74-75 2.5851730501913934E-5 5.170346100382787E-5 0.0 18.857441407699113 8.272553760612459E-4 76-77 2.5851730501913934E-5 5.170346100382787E-5 0.0 19.94961239207872 8.401812413122028E-4 78-79 2.5851730501913934E-5 5.170346100382787E-5 0.0 21.10133283766949 8.531071065631597E-4 80-81 2.5851730501913934E-5 5.170346100382787E-5 0.0 22.238110983030147 8.531071065631597E-4 82-83 2.5851730501913934E-5 5.170346100382787E-5 0.0 23.355500330514374 9.306622980689016E-4 84-85 2.5851730501913934E-5 5.170346100382787E-5 0.0 24.430609172762722 9.306622980689016E-4 86-87 2.5851730501913934E-5 5.170346100382787E-5 0.0 25.484093042446215 9.565140285708155E-4 88 2.5851730501913934E-5 7.75551915057418E-5 0.0 26.288598895665775 9.823657590727294E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7010 0.0 56.648323 1 GTATCAA 12865 0.0 40.899773 1 TACCTGG 3020 0.0 39.426807 2 ATCAACG 15140 0.0 34.05845 3 CAACGCA 15210 0.0 33.870796 5 TATCAAC 15435 0.0 33.63155 2 TCAACGC 15840 0.0 32.55334 4 AACGCAG 16200 0.0 32.119675 6 ACGCAGA 17900 0.0 28.72857 7 ACCTGGG 4085 0.0 28.651764 3 CGCAGAG 18285 0.0 27.943365 8 GTACATG 12880 0.0 27.246952 1 ATAACGC 380 0.0 27.213423 3 GTGGTAT 2565 0.0 25.894604 1 TACATGG 13180 0.0 25.816711 2 GCAGAGT 19600 0.0 25.156294 9 ACATGGG 12865 0.0 25.137537 3 GTACTAG 1120 0.0 24.394253 1 TAACGCA 450 0.0 24.02478 4 CCTGGGG 3955 0.0 23.532215 4 >>END_MODULE