FastQCFastQC Report
Mon 6 Jun 2016
ERR1211459_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211459_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3868213
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143040.36978315309937687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123800.3200444236136945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70830.1831078071450564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38750.10017545569491648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69750.055.541291
TACCTGG29450.042.127012
GTATCAA121900.039.4938241
ATCAACG149500.031.6541233
CAACGCA151000.031.3647175
ACCTGGG38250.031.2064463
TATCAAC153400.031.1557082
TCAACGC156600.030.2789944
AACGCAG160100.029.934136
TAACGCA4400.026.701114
GTACTAG10950.026.6201521
ATAACGC4950.026.582443
GTGGTAT27900.026.4562551
GTACATG126600.026.2925381
ACGCAGA179950.026.1099457
CGCAGAG184950.025.3786778
TACATGG129900.025.2515622
ACATGGG126850.024.5620773
CATGGGG94600.023.8943884
CCTGGGG37350.023.4026134
Produced by FastQC (version 0.11.5)