FastQCFastQC Report
Mon 6 Jun 2016
ERR1211458_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211458_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4601148
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223890.4865959538793362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179110.3892724163621774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103460.2248569270103896No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57900.12583816038953755No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57880.12579469297662238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83650.057.4509051
GTATCAA159600.043.8975941
TACCTGG36450.041.064492
ATCAACG196500.035.0210763
TATCAAC198650.034.949452
CAACGCA198050.034.746995
TATCACG2450.034.581462
TCAACGC208300.033.1048624
AACGCAG210500.032.9598466
ACCTGGG44850.032.8044553
ACGCAGA231700.029.7009747
TAACGCA4150.029.4493834
CGCAGAG234600.029.2126548
GTACTAG17300.027.234321
GTACATG153250.026.5629271
GCAGAGT251750.026.419169
GTGGTAT32500.026.384611
TACATGG155350.025.4813022
CATGGGG107200.024.6429394
ACATGGG154050.024.288593
Produced by FastQC (version 0.11.5)