##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211458_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4601148 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.39004678832326 31.0 31.0 34.0 29.0 34.0 2 31.43526376460831 31.0 31.0 34.0 28.0 34.0 3 31.50065440190144 31.0 31.0 34.0 28.0 34.0 4 35.13632684712598 37.0 35.0 37.0 32.0 37.0 5 35.193956377843094 37.0 35.0 37.0 32.0 37.0 6 35.26038936369793 37.0 35.0 37.0 32.0 37.0 7 35.19619603629355 37.0 35.0 37.0 32.0 37.0 8 35.20542156001068 37.0 35.0 37.0 32.0 37.0 9 36.77819622407277 39.0 37.0 39.0 32.0 39.0 10-11 36.708825927790194 39.0 37.0 39.0 32.0 39.0 12-13 36.62414097525226 39.0 37.0 39.0 32.0 39.0 14-15 37.80429775351716 40.0 37.0 41.0 32.0 41.0 16-17 37.79941907976009 40.0 37.0 41.0 32.0 41.0 18-19 37.81375745792137 40.0 37.0 41.0 32.0 41.0 20-21 37.80019258237292 40.0 37.0 41.0 32.0 41.0 22-23 37.668658669532036 40.0 37.0 41.0 32.0 41.0 24-25 37.57088100621845 40.0 37.0 41.0 32.0 41.0 26-27 37.42725619780107 40.0 37.0 41.0 31.5 41.0 28-29 37.29536998157851 40.0 36.0 41.0 31.0 41.0 30-31 37.121141397755515 39.0 36.0 41.0 31.0 41.0 32-33 36.90188492089366 39.0 35.5 41.0 30.0 41.0 34-35 36.75752573053508 39.0 35.0 41.0 30.0 41.0 36-37 36.60711707165255 39.0 35.0 41.0 30.0 41.0 38-39 36.4399358594855 39.0 35.0 41.0 30.0 41.0 40-41 36.26470459111509 39.0 35.0 40.0 29.0 41.0 42-43 36.04525957434971 38.5 35.0 40.0 28.0 41.0 44-45 35.88356318901283 38.0 35.0 40.0 27.5 41.0 46-47 35.62481298145593 38.0 34.5 40.0 26.5 41.0 48-49 35.35460139513008 38.0 34.0 40.0 26.0 41.0 50-51 34.67812478537965 37.5 33.5 39.5 25.0 40.5 52-53 34.97150602414875 38.0 34.0 39.5 26.0 40.5 54-55 35.36799022765623 38.0 34.0 40.0 26.0 41.0 56-57 35.28273759070562 38.0 34.0 40.0 26.0 41.0 58-59 35.07886586130244 38.0 34.0 40.0 26.0 41.0 60-61 34.84975445258444 38.0 34.0 40.0 25.0 41.0 62-63 34.57370432335583 37.0 33.5 40.0 25.0 41.0 64-65 34.36843044388053 37.0 33.0 40.0 24.5 41.0 66-67 34.16957268055711 36.5 33.0 40.0 24.0 41.0 68-69 33.821957476699296 36.0 33.0 39.0 23.0 41.0 70-71 33.446535408119885 35.5 32.5 39.0 23.0 41.0 72-73 33.051801311324915 35.0 32.0 38.5 22.0 40.0 74-75 32.65818421837333 35.0 32.0 37.5 20.5 40.0 76-77 32.25590385269068 35.0 31.0 37.0 20.0 39.0 78-79 31.845052582529405 35.0 31.0 36.5 19.5 39.0 80-81 31.43181842879212 35.0 31.0 36.0 18.5 38.5 82-83 31.043075880193378 35.0 30.0 35.5 18.0 37.0 84-85 30.692606279997946 34.0 30.0 35.0 16.5 37.0 86-87 30.339233165288313 34.0 29.0 35.0 14.5 36.5 88-89 30.009398198014928 34.0 29.0 35.0 11.5 36.0 90-91 29.684970359571132 34.0 29.0 35.0 10.0 36.0 92-93 29.375870326275095 34.0 28.5 35.0 10.0 35.0 94-95 29.07471200665573 34.0 27.0 35.0 10.0 35.0 96-97 28.771925941091226 34.0 26.5 35.0 9.0 35.0 98-99 28.443233623434846 34.0 25.0 35.0 8.0 35.0 100 27.199750149310564 32.0 23.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.38547561327834856 1101 2 -0.285475888059036 1101 3 -0.4126431286867671 1101 4 -0.29850865297117224 1101 5 -0.2800778151023735 1101 6 -0.2490990924632328 1101 7 -0.31559228437385656 1101 8 -0.25768411730634 1101 9 -0.3775199265623499 1101 10-11 -0.3876967159891649 1101 12-13 -0.4725484751636415 1101 14-15 -0.615386010496465 1101 16-17 -0.4699962102695565 1101 18-19 -0.5395985917893782 1101 20-21 -0.6433311222679237 1101 22-23 -0.6650233092152504 1101 24-25 -0.7791337342084077 1101 26-27 -0.7873093709305508 1101 28-29 -0.8082277680024319 1101 30-31 -0.8822083553973954 1101 32-33 -0.8060544595272532 1101 34-35 -0.7580258553903505 1101 36-37 -0.8276540455223227 1101 38-39 -0.9625685187491015 1101 40-41 -1.049093091576836 1101 42-43 -1.0924355020290974 1101 44-45 -0.9876954775759756 1101 46-47 -0.9172940236471661 1101 48-49 -1.0718917683781584 1101 50-51 -0.9843423775625126 1101 52-53 -0.7525296066270641 1101 54-55 -0.6251354998518153 1101 56-57 -0.6121569401434925 1101 58-59 -0.7068333874763226 1101 60-61 -0.6478411337862582 1101 62-63 -0.811003288235284 1101 64-65 -0.7248754978801557 1101 66-67 -0.6710169451652703 1101 68-69 -0.7656552451554361 1101 70-71 -0.7081989205826318 1101 72-73 -0.9075893291030326 1101 74-75 -0.9699199952036253 1101 76-77 -0.9241022833408437 1101 78-79 -1.0989358661178343 1101 80-81 -1.0382181076815016 1101 82-83 -1.1216634592266708 1101 84-85 -1.213637558287239 1101 86-87 -1.3720193048506069 1101 88-89 -1.5980908035878265 1101 90-91 -1.7197972573210336 1101 92-93 -1.6153963937340592 1101 94-95 -1.830551974592634 1101 96-97 -1.8987055878769645 1101 98-99 -2.047384687633965 1101 100 -2.0081204032337894 1102 1 -0.40482303418930954 1102 2 -0.3679650567924 1102 3 -0.437394664061344 1102 4 -0.36537778367223694 1102 5 -0.35131767142269155 1102 6 -0.30974497053303907 1102 7 -0.43268597955592725 1102 8 -0.4117744208653491 1102 9 -0.49932955854502126 1102 10-11 -0.47311604479088487 1102 12-13 -0.4998031755647574 1102 14-15 -0.6968450688284094 1102 16-17 -0.7168788245271287 1102 18-19 -0.7204391881044785 1102 20-21 -0.7225261351595904 1102 22-23 -0.6773841909994402 1102 24-25 -0.7968271954218906 1102 26-27 -0.823991780424258 1102 28-29 -0.8018024924005758 1102 30-31 -0.6885254187536844 1102 32-33 -0.8011785288038809 1102 34-35 -0.9552233714765705 1102 36-37 -0.7511598198350242 1102 38-39 -0.976171841802504 1102 40-41 -1.1742347439404313 1102 42-43 -1.1294256125911843 1102 44-45 -0.9857303906895609 1102 46-47 -1.1292309466412078 1102 48-49 -1.227988479018883 1102 50-51 -0.9721738310977059 1102 52-53 -0.8331510080190583 1102 54-55 -0.8926830555865237 1102 56-57 -0.8478599753555045 1102 58-59 -0.798793918803824 1102 60-61 -0.8975378925816955 1102 62-63 -0.877344766835499 1102 64-65 -1.0265299360530875 1102 66-67 -0.9761125965440343 1102 68-69 -0.9834103115607391 1102 70-71 -0.9631338928145112 1102 72-73 -0.9855377362168838 1102 74-75 -1.2203964917803418 1102 76-77 -1.2167932812917854 1102 78-79 -1.351974214803338 1102 80-81 -1.3667087279085592 1102 82-83 -1.481083116935963 1102 84-85 -1.5222083296511073 1102 86-87 -1.6632225394864655 1102 88-89 -1.7867074235332332 1102 90-91 -1.8085809183647683 1102 92-93 -1.861364824514137 1102 94-95 -1.9044292348870648 1102 96-97 -2.180933184120235 1102 98-99 -2.1852374244663224 1102 100 -2.176929556977285 1103 1 -0.416451070326751 1103 2 -0.4914821628350552 1103 3 -0.5783901561377647 1103 4 -0.3967157205798202 1103 5 -0.325034486810722 1103 6 -0.37248656561597926 1103 7 -0.44034204442559854 1103 8 -0.4617677098908217 1103 9 -0.4923008540870839 1103 10-11 -0.504272553973351 1103 12-13 -0.6300688607321163 1103 14-15 -0.7494765807878281 1103 16-17 -0.7821096126269538 1103 18-19 -0.8355547325727883 1103 20-21 -0.7350321518953749 1103 22-23 -0.7298252501300695 1103 24-25 -0.7740447096769216 1103 26-27 -0.8105170692275792 1103 28-29 -0.8417563949479714 1103 30-31 -0.8628590652658303 1103 32-33 -0.8897648967319043 1103 34-35 -0.8053184821845107 1103 36-37 -0.9039245099268527 1103 38-39 -0.869376054443201 1103 40-41 -0.9424677674890063 1103 42-43 -0.983754191160493 1103 44-45 -0.955853942044719 1103 46-47 -1.0695474732259243 1103 48-49 -1.1113251292706607 1103 50-51 -1.1728929467301086 1103 52-53 -0.8576365771097016 1103 54-55 -0.8245552092076736 1103 56-57 -0.8292233676837029 1103 58-59 -0.8256990256240258 1103 60-61 -0.9143811658710916 1103 62-63 -0.7840024006847344 1103 64-65 -0.8742468162367416 1103 66-67 -1.0182185234916048 1103 68-69 -1.004393214774602 1103 70-71 -0.9541539146598907 1103 72-73 -0.9096159028712094 1103 74-75 -0.9820450079607355 1103 76-77 -1.1028071475536336 1103 78-79 -1.0278604105601516 1103 80-81 -0.9928082863727212 1103 82-83 -0.9590535331089498 1103 84-85 -1.2069940875746852 1103 86-87 -1.361500827401155 1103 88-89 -1.2201741278430696 1103 90-91 -1.4307442588564747 1103 92-93 -1.5436637035499778 1103 94-95 -1.4087906740902625 1103 96-97 -1.5726336756412636 1103 98-99 -1.6818627299313675 1103 100 -1.5829219944032324 1104 1 -0.4560824272965043 1104 2 -0.5147757385282752 1104 3 -0.5515012296169317 1104 4 -0.3381222119375664 1104 5 -0.24068448074638837 1104 6 -0.30357956508277084 1104 7 -0.4368841137604207 1104 8 -0.48752226214824645 1104 9 -0.49194179957441264 1104 10-11 -0.4555912039833032 1104 12-13 -0.5266761502939019 1104 14-15 -0.6656789442773174 1104 16-17 -0.7514141132802976 1104 18-19 -0.7681472251525534 1104 20-21 -0.690534164568696 1104 22-23 -0.725529582812726 1104 24-25 -0.7066347994062241 1104 26-27 -0.7150746472458422 1104 28-29 -0.9308403024862812 1104 30-31 -0.8943217604402207 1104 32-33 -0.9139423209219686 1104 34-35 -0.8048566772090098 1104 36-37 -1.0024578557702597 1104 38-39 -1.0057192821807988 1104 40-41 -0.9772272879258281 1104 42-43 -0.9675049720868998 1104 44-45 -1.0235342874144209 1104 46-47 -1.0542637423543297 1104 48-49 -1.0658537586618664 1104 50-51 -0.9764214182197151 1104 52-53 -0.8775995732125068 1104 54-55 -0.890042953526077 1104 56-57 -0.7519741280142398 1104 58-59 -0.8362131555289878 1104 60-61 -0.9118223589664467 1104 62-63 -0.8968078201423211 1104 64-65 -0.9251039473248142 1104 66-67 -0.7721722423031778 1104 68-69 -0.9308005414089351 1104 70-71 -0.9772201349303344 1104 72-73 -0.9137262646489503 1104 74-75 -0.7913206400820378 1104 76-77 -0.9321851983155653 1104 78-79 -0.968890283712529 1104 80-81 -0.9059574278685716 1104 82-83 -1.1153559810414677 1104 84-85 -1.2770867803919934 1104 86-87 -1.2211787653435415 1104 88-89 -1.3026194744370834 1104 90-91 -1.2695615644143814 1104 92-93 -1.1715816793887441 1104 94-95 -1.3493709853623805 1104 96-97 -1.4263653024168015 1104 98-99 -1.4274608795076702 1104 100 -1.4751360723608542 1105 1 -0.5454246602663808 1105 2 -0.5070651420604726 1105 3 -0.5399172479456773 1105 4 -0.352696495525997 1105 5 -0.45532052584616167 1105 6 -0.36908247876066014 1105 7 -0.4134749523000636 1105 8 -0.44603930631442523 1105 9 -0.5555182487451802 1105 10-11 -0.5030856212927972 1105 12-13 -0.5985619150745407 1105 14-15 -0.7630802801473706 1105 16-17 -0.8242721249063436 1105 18-19 -0.769431573347866 1105 20-21 -0.7278392615807192 1105 22-23 -0.6865850435866534 1105 24-25 -0.7329506466657563 1105 26-27 -0.7707988090563802 1105 28-29 -0.6889854485099605 1105 30-31 -0.7429954828356315 1105 32-33 -0.9516642272353977 1105 34-35 -0.8885587227585816 1105 36-37 -0.8693455032137365 1105 38-39 -0.8167217764490147 1105 40-41 -0.8106199480602214 1105 42-43 -0.8965735405396629 1105 44-45 -0.9849960404659228 1105 46-47 -1.0384387997183424 1105 48-49 -0.8818373927859326 1105 50-51 -1.07121574568378 1105 52-53 -0.9039654630139751 1105 54-55 -0.7459429114348666 1105 56-57 -0.6631655473247449 1105 58-59 -0.6207766609015692 1105 60-61 -0.7338993371088378 1105 62-63 -0.7096418367740185 1105 64-65 -0.7453951694931291 1105 66-67 -0.7012769269091947 1105 68-69 -0.7131562229941366 1105 70-71 -0.6224950788260628 1105 72-73 -0.6582106635109 1105 74-75 -0.7119514349910574 1105 76-77 -0.688558283610476 1105 78-79 -0.7347080031178734 1105 80-81 -0.8713634693555719 1105 82-83 -1.0240981473033628 1105 84-85 -0.9826737932775416 1105 86-87 -0.9819026047090595 1105 88-89 -1.037365282594088 1105 90-91 -1.0553929490163938 1105 92-93 -1.1227198178385436 1105 94-95 -1.26730202558387 1105 96-97 -1.1645822485088502 1105 98-99 -1.184622105945202 1105 100 -1.2577361302853873 1106 1 -0.46842572099742696 1106 2 -0.6552041596521789 1106 3 -0.5868564736208448 1106 4 -0.37638663399511785 1106 5 -0.4206851399997191 1106 6 -0.4765641875533362 1106 7 -0.4656620808013159 1106 8 -0.413175301573375 1106 9 -0.6098099480168813 1106 10-11 -0.7273069753720591 1106 12-13 -0.5452462142236598 1106 14-15 -0.7464619211981045 1106 16-17 -0.6954597639009705 1106 18-19 -0.8016330627873671 1106 20-21 -0.834202002894834 1106 22-23 -0.6809557611389678 1106 24-25 -0.763348849163819 1106 26-27 -0.8048028678320023 1106 28-29 -0.8791022790703167 1106 30-31 -0.7583554932751326 1106 32-33 -0.7223602955531945 1106 34-35 -0.705636879029619 1106 36-37 -0.7284096973142695 1106 38-39 -0.788413737414821 1106 40-41 -0.763291117902611 1106 42-43 -0.729432279161756 1106 44-45 -0.7830445735481888 1106 46-47 -0.7463274569617795 1106 48-49 -0.8076206645008952 1106 50-51 -0.850139103670358 1106 52-53 -0.8205538923018025 1106 54-55 -0.7517774290009598 1106 56-57 -0.8470617886626997 1106 58-59 -0.7316097520891134 1106 60-61 -0.6134847798337688 1106 62-63 -0.7043684313592493 1106 64-65 -0.8030142389820512 1106 66-67 -0.7337732969303303 1106 68-69 -0.6155030021165473 1106 70-71 -0.664149914205197 1106 72-73 -0.6572330090778138 1106 74-75 -0.801037221283341 1106 76-77 -0.7164563492115263 1106 78-79 -0.7572562107385323 1106 80-81 -0.8579516469295463 1106 82-83 -0.8216944234396522 1106 84-85 -0.8840905007189122 1106 86-87 -0.8141536972355432 1106 88-89 -0.8298590537800017 1106 90-91 -0.9019831756295282 1106 92-93 -0.8852315976056957 1106 94-95 -0.9255451467297782 1106 96-97 -0.9380533517574605 1106 98-99 -1.122298692051654 1106 100 -1.0516043364399223 1107 1 -0.5504624752053822 1107 2 -0.5478745537786232 1107 3 -0.6621738443318677 1107 4 -0.5103626699380257 1107 5 -0.5154875260258791 1107 6 -0.48482053920410806 1107 7 -0.49821119303181405 1107 8 -0.5118923147193968 1107 9 -0.6986235969168675 1107 10-11 -0.6797138400692972 1107 12-13 -0.7159901474810155 1107 14-15 -0.8139077515834998 1107 16-17 -0.8315605896049902 1107 18-19 -0.922528902916838 1107 20-21 -0.8330448681173976 1107 22-23 -0.7905615340767227 1107 24-25 -0.8518468219736306 1107 26-27 -0.8817968805247176 1107 28-29 -1.000629047724921 1107 30-31 -1.0224958068223557 1107 32-33 -0.9472326164635447 1107 34-35 -0.9067454523283232 1107 36-37 -0.8639162936259268 1107 38-39 -0.8221976192404341 1107 40-41 -0.8017954455406056 1107 42-43 -0.8013748918858212 1107 44-45 -0.9404610287402946 1107 46-47 -0.946212090351743 1107 48-49 -0.9115627762526657 1107 50-51 -0.9770069465451101 1107 52-53 -0.8230573768659326 1107 54-55 -0.7658361359628927 1107 56-57 -0.7450759313328277 1107 58-59 -0.7912140951905187 1107 60-61 -0.8760736150361339 1107 62-63 -0.7460415940725156 1107 64-65 -0.8057114398920646 1107 66-67 -0.8310558135270298 1107 68-69 -0.7478484685821201 1107 70-71 -0.7972271920933593 1107 72-73 -0.8376329448940538 1107 74-75 -0.6930634211808169 1107 76-77 -0.838758089163484 1107 78-79 -0.755153901182144 1107 80-81 -0.8611835536745609 1107 82-83 -0.7839832048377993 1107 84-85 -0.9299555199316742 1107 86-87 -0.9091925093486921 1107 88-89 -0.7885748330302214 1107 90-91 -0.8843329051256745 1107 92-93 -0.8978487117934826 1107 94-95 -0.8779440414133255 1107 96-97 -0.9315355751847463 1107 98-99 -0.8875984969584287 1107 100 -0.9705420421774846 1108 1 -0.5081201090066187 1108 2 -0.4576094355032936 1108 3 -0.48368833172590797 1108 4 -0.4212541520639945 1108 5 -0.3837765549969916 1108 6 -0.42988356703052943 1108 7 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48-49 1.3846690969094837 2311 50-51 1.287061106428304 2311 52-53 1.140723416087262 2311 54-55 1.144595357215799 2311 56-57 1.1261726027405956 2311 58-59 1.1235969558464518 2311 60-61 1.1782321845750516 2311 62-63 1.1363963400509434 2311 64-65 1.061200457548928 2311 66-67 1.1665768504390641 2311 68-69 1.151422422756454 2311 70-71 1.1082311305680719 2311 72-73 1.1235257880356784 2311 74-75 1.1597734998233165 2311 76-77 1.1265858561827855 2311 78-79 1.1844308736545095 2311 80-81 1.1964206816161607 2311 82-83 1.3628775141914886 2311 84-85 1.296127290637692 2311 86-87 1.3497274813237432 2311 88-89 1.3939690130938942 2311 90-91 1.4153602537703591 2311 92-93 1.449349281846274 2311 94-95 1.5294854295193794 2311 96-97 1.5771915142039639 2311 98-99 1.6094097810402914 2311 100 1.6512222686851743 2312 1 0.5366430568498188 2312 2 0.5409345313858935 2312 3 0.6396911386160724 2312 4 0.4927841735767444 2312 5 0.5275354966916979 2312 6 0.3610546932035774 2312 7 0.5176975077779602 2312 8 0.5515191398062527 2312 9 0.7333863846939508 2312 10-11 0.7750350112953086 2312 12-13 0.7976634306119905 2312 14-15 0.9894265226115806 2312 16-17 0.9641052163003252 2312 18-19 0.9750081795386762 2312 20-21 0.9989666392151761 2312 22-23 0.9892909922006581 2312 24-25 1.0067529299270674 2312 26-27 1.0035041676885115 2312 28-29 1.0512419745383355 2312 30-31 1.008695731016175 2312 32-33 1.0876044216188987 2312 34-35 0.9883925809908973 2312 36-37 1.093256993189847 2312 38-39 1.1787680990630136 2312 40-41 1.2095321485360344 2312 42-43 1.2588523389418995 2312 44-45 1.343238647340506 2312 46-47 1.218124566088349 2312 48-49 1.324505540482086 2312 50-51 1.246145881189463 2312 52-53 1.125200079357512 2312 54-55 1.0871204585244243 2312 56-57 1.07953355806319 2312 58-59 1.1757096979741561 2312 60-61 1.065898015862956 2312 62-63 1.0829192961691874 2312 64-65 1.0622350530457467 2312 66-67 1.0866214954727411 2312 68-69 1.050761535321179 2312 70-71 1.1206874514026808 2312 72-73 1.2212680734828467 2312 74-75 1.2363617334786312 2312 76-77 1.1560713669294245 2312 78-79 1.1456339010114185 2312 80-81 0.9483800729491207 2312 82-83 1.2293140112709438 2312 84-85 1.223494200543385 2312 86-87 1.267602661233358 2312 88-89 1.430984814002528 2312 90-91 1.436163704593426 2312 92-93 1.507229886186881 2312 94-95 1.5523564325351842 2312 96-97 1.5577256321951722 2312 98-99 1.4479266416385599 2312 100 1.5893143111186454 2313 1 0.5891359047641771 2313 2 0.6084611786727159 2313 3 0.6460616858659805 2313 4 0.5217270491765973 2313 5 0.5182147141150963 2313 6 0.574498307083239 2313 7 0.6088519928277947 2313 8 0.6185728265910484 2313 9 0.7294642732137007 2313 10-11 0.6776152415463201 2313 12-13 0.7746988911140207 2313 14-15 0.9794727227777003 2313 16-17 0.911649859157464 2313 18-19 0.9143235980510411 2313 20-21 0.9941231199276146 2313 22-23 1.0128496162316694 2313 24-25 1.0312041227103776 2313 26-27 1.090184273354815 2313 28-29 1.1132819916110535 2313 30-31 1.1144672910937032 2313 32-33 1.1289149259562947 2313 34-35 1.1308560707472637 2313 36-37 1.0932771805283465 2313 38-39 1.1513291801815129 2313 40-41 1.1203805358560786 2313 42-43 1.1668759408245108 2313 44-45 1.1902921610909587 2313 46-47 1.1500716060477458 2313 48-49 1.0898882924200706 2313 50-51 0.9818056610897941 2313 52-53 0.8574092663268686 2313 54-55 0.8301861077492347 2313 56-57 0.8803263436851907 2313 58-59 0.8893277881365833 2313 60-61 0.9149933347075461 2313 62-63 0.9451508045671133 2313 64-65 0.881275750720171 2313 66-67 0.8955858273295192 2313 68-69 0.9527894976689097 2313 70-71 0.9234542703395618 2313 72-73 0.9478147120025966 2313 74-75 0.9012735436484363 2313 76-77 0.8992019040279828 2313 78-79 0.940157076076023 2313 80-81 0.978281847127775 2313 82-83 1.0185308002151992 2313 84-85 1.0673912797238927 2313 86-87 1.204510018653071 2313 88-89 1.2494083558999058 2313 90-91 1.4046844341061622 2313 92-93 1.4827570641123167 2313 94-95 1.423179095418167 2313 96-97 1.4863230159161027 2313 98-99 1.6112912365075154 2313 100 1.6659858227465527 2314 1 0.40894878450438554 2314 2 0.4335052416310248 2314 3 0.470842156743835 2314 4 0.3601208790529853 2314 5 0.3806010539433018 2314 6 0.4108698239489428 2314 7 0.4873566020882194 2314 8 0.47440282082946794 2314 9 0.6765049185101617 2314 10-11 0.6176430346010022 2314 12-13 0.3887646647364775 2314 14-15 0.7189284830437757 2314 16-17 0.7340899518664514 2314 18-19 0.7723766110931578 2314 20-21 0.8034104850543784 2314 22-23 0.7178642165878344 2314 24-25 0.7150757377847512 2314 26-27 0.7881270962649296 2314 28-29 0.8986750554597833 2314 30-31 0.8554860096669188 2314 32-33 0.8981307951685267 2314 34-35 0.9603802497227534 2314 36-37 0.9195437450579931 2314 38-39 1.0488516044953542 2314 40-41 0.9949665862960586 2314 42-43 1.0735507915473264 2314 44-45 1.046025354313251 2314 46-47 1.0329844425761294 2314 48-49 1.0348323789484581 2314 50-51 0.94469276694565 2314 52-53 0.8154503961194592 2314 54-55 0.8137326521133659 2314 56-57 0.7374727597233246 2314 58-59 0.7252477742564238 2314 60-61 0.6975081642172896 2314 62-63 0.7143488652731733 2314 64-65 0.6787257297689777 2314 66-67 0.7469593083686945 2314 68-69 0.7929416556408384 2314 70-71 0.8375753027091406 2314 72-73 0.8666451449064283 2314 74-75 0.90814477898261 2314 76-77 0.9367714357690247 2314 78-79 1.0005706982738012 2314 80-81 1.0883831657429042 2314 82-83 1.0569739635820525 2314 84-85 1.0222871130572244 2314 86-87 1.2695603997027227 2314 88-89 1.4541706909093328 2314 90-91 1.4898750571419868 2314 92-93 1.6810907902803471 2314 94-95 1.7161486965600083 2314 96-97 1.8559133548015012 2314 98-99 1.8744570258433626 2314 100 1.8135632111814992 2315 1 0.4116427560089271 2315 2 0.4307866640225768 2315 3 0.48378972986762037 2315 4 0.40775908995111365 2315 5 0.3414162303531896 2315 6 -0.27772008754845956 2315 7 0.27836664296609825 2315 8 0.2959926926396079 2315 9 0.5826683506647825 2315 10-11 0.6163238299337834 2315 12-13 0.6505545603589766 2315 14-15 0.7614684679626151 2315 16-17 0.6506300161598233 2315 18-19 0.7805894935791642 2315 20-21 0.7679927765970191 2315 22-23 0.6214594858659197 2315 24-25 0.8507553821868683 2315 26-27 0.8744445150052584 2315 28-29 0.9595481752756356 2315 30-31 0.9612986064046254 2315 32-33 1.0465468764480477 2315 34-35 0.9588753086583424 2315 36-37 1.048486079462883 2315 38-39 1.106861288187865 2315 40-41 1.02050653152763 2315 42-43 1.0847509344214785 2315 44-45 1.1227364707159992 2315 46-47 1.1672940826126492 2315 48-49 1.1447807855047998 2315 50-51 1.01478404765367 2315 52-53 0.8748443715927294 2315 54-55 0.6589884365321481 2315 56-57 0.6687760894848083 2315 58-59 0.5985132423677086 2315 60-61 0.5748983297131787 2315 62-63 0.7223406896967148 2315 64-65 0.8054642303841391 2315 66-67 0.7621746117778301 2315 68-69 0.7933254314871689 2315 70-71 0.9238165219018981 2315 72-73 0.9519136292498018 2315 74-75 0.9060203197215841 2315 76-77 0.9080311095397064 2315 78-79 0.9524288559909913 2315 80-81 0.9866218449507471 2315 82-83 1.1269735667094096 2315 84-85 1.2265308722349069 2315 86-87 1.2735430166809358 2315 88-89 1.4540921101259094 2315 90-91 1.5624575074336917 2315 92-93 1.6772145351706094 2315 94-95 1.791389399818332 2315 96-97 1.8661284597743162 2315 98-99 2.0053230019516306 2315 100 2.0368278855474955 2316 1 -0.09813441243663235 2316 2 -0.09521219618594046 2316 3 0.003170314285263487 2316 4 -0.04964708796616435 2316 5 -0.04366710046703304 2316 6 0.08676391709422404 2316 7 0.1400248153251482 2316 8 0.13244854427803432 2316 9 0.20838371673966805 2316 10-11 0.05392035457501265 2316 12-13 0.1776018431471229 2316 14-15 0.39271667882249517 2316 16-17 0.4308884565322586 2316 18-19 0.3605309265460477 2316 20-21 0.32911125691628484 2316 22-23 0.2020906705299339 2316 24-25 0.28501268895393395 2316 26-27 0.2860118056188341 2316 28-29 0.4195960712623119 2316 30-31 0.43568402759005664 2316 32-33 0.4985491281934955 2316 34-35 0.4719063730024331 2316 36-37 0.6019294482967794 2316 38-39 0.5994145370962016 2316 40-41 0.6308164171695836 2316 42-43 0.6131301339228798 2316 44-45 0.48346938602269063 2316 46-47 0.4892378523840435 2316 48-49 0.5337731680788238 2316 50-51 0.35879478365430373 2316 52-53 0.23874748467720508 2316 54-55 0.19228698844020897 2316 56-57 0.32268114346251764 2316 58-59 0.35899028453921744 2316 60-61 0.47131305915962685 2316 62-63 0.43514364109164916 2316 64-65 0.5287372104439712 2316 66-67 0.5059784222693224 2316 68-69 0.5139409962136412 2316 70-71 0.40730825841365714 2316 72-73 0.515204177873045 2316 74-75 0.6037413708351238 2316 76-77 0.6944721222374852 2316 78-79 0.8350981641282793 2316 80-81 0.9836222911242061 2316 82-83 0.9793526455815886 2316 84-85 1.1239739657867318 2316 86-87 1.1711084406346508 2316 88-89 1.2121850085445054 2316 90-91 1.2576750846576559 2316 92-93 1.4021718239206216 2316 94-95 1.558986226032605 2316 96-97 1.684914156551116 2316 98-99 1.845287524588553 2316 100 1.7261529573257484 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 10.0 1 275.0 2 120.0 3 77.0 4 85.0 5 79.0 6 94.0 7 687.0 8 2406.0 9 3302.0 10 4111.0 11 4941.0 12 6098.0 13 7306.0 14 8910.0 15 11431.0 16 15620.0 17 21394.0 18 27375.0 19 34030.0 20 41519.0 21 49330.0 22 53303.0 23 53193.0 24 51918.0 25 53460.0 26 59315.0 27 67896.0 28 79536.0 29 95172.0 30 114861.0 31 140821.0 32 176331.0 33 225082.0 34 305979.0 35 416370.0 36 589465.0 37 841995.0 38 813491.0 39 223484.0 40 276.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.86024444262446 19.457849852843033 12.40470518943955 23.277200515092954 2 16.193810383995796 21.283114541394525 39.813544908268895 22.709530166340787 3 18.9014472501725 27.15299495612424 30.365971208038893 23.579586585664366 4 12.509486237754302 17.4705467398972 38.938932650157554 31.081034372190945 5 12.667853080618178 37.649309021847635 35.09444643484492 14.588391462689268 6 28.646351946449823 37.74694184299015 19.207311437843188 14.399394772716844 7 25.65706290153723 33.463179381097135 23.48400462261209 17.395753094753545 8 25.288506347259244 34.60693137192436 21.573679287974446 18.530882992841953 9 26.640666396618705 15.848140854582216 21.412911814989748 36.09828093380933 10-11 24.256069575563494 26.18807302146557 29.237842040788102 20.318015362182834 12-13 24.989454618985597 24.729896916182703 28.747676211142448 21.532972253689252 14-15 22.51281437596959 25.94295536209314 27.08535022736489 24.458880034572374 16-17 21.368863164875997 28.4588159710557 27.88164514607738 22.290675717990922 18-19 20.879296691333828 27.74929662394711 30.312350485716948 21.05905619900211 20-21 21.869519533571175 27.18604817739965 29.978927835853852 20.965504453175328 22-23 21.919818191640296 27.056373917569935 29.652325642441745 21.37148224834802 24-25 21.428060572332896 26.996426180186607 30.093280175299775 21.482233072180726 26-27 21.318334488712605 27.157369178567727 29.87820585141453 21.646090481305137 28-29 21.139219360168667 27.720325488140368 29.779086777237218 21.361368374453743 30-31 21.805819937096214 27.3300844564522 29.791446711006685 21.0726488954449 32-33 21.66597644552416 27.445703509258202 29.452116587590147 21.436203457627485 34-35 21.755161792992624 27.345785347325364 29.763571445962917 21.135481413719102 36-37 21.668095826032193 27.109285419954922 29.692533092493985 21.530085661518903 38-39 21.499655766033072 27.180027082202464 29.855780424163203 21.46453672760126 40-41 21.494464653860295 26.676715155134367 30.233508335872266 21.59531185513307 42-43 21.245766621432363 26.997813361323235 29.672641189072284 22.083778828172115 44-45 20.90314670581477 27.36647601388563 29.93762510093958 21.792752179360015 46-47 20.865347149501982 27.019240470218236 29.661684919687865 22.453727460591914 48-49 21.029332547696715 26.974898583842883 29.595482299530847 22.400286568929555 50-51 20.966138435507325 27.150101841008023 29.429214249175565 22.454545474309086 52-53 20.682457711411413 27.493945261050378 29.187239153192152 22.636357874346054 54-55 20.569363912055824 27.213838156579424 29.443350345470133 22.773447585894623 56-57 20.967499775225832 27.105970882531334 29.039898474916782 22.886630867326055 58-59 20.750775942710053 27.105112893847732 29.204072171695888 22.940038991746327 60-61 20.952346998036635 27.09326242341192 28.97162606770044 22.982764510851 62-63 21.1069089033977 26.785867625456987 28.936374053443476 23.170849417701838 64-65 20.839232883478367 26.690597481220895 28.886219281305987 23.583950353994748 66-67 21.135191196681415 26.55553126639722 28.518757142489104 23.790520394432257 68-69 20.944335077705343 26.679075373764107 28.60537822006656 23.77121132846399 70-71 21.146454493311747 26.461866240163083 28.545126192094468 23.8465530744307 72-73 21.45152926160794 26.544519978300173 28.3364408152438 23.667509944848085 74-75 21.613191796486074 26.537289292562143 28.208003078274253 23.641515832677534 76-77 21.654974315811074 26.41679316201266 28.217131878979224 23.711100643197042 78-79 21.751310066497272 26.494645285923497 28.172695533959562 23.58134911361967 80-81 21.96735409289009 26.4021711331419 28.057998184200894 23.572476589767113 82-83 22.36804564597178 26.06231432493865 28.338743698881448 23.23089633020812 84-85 22.565522383112285 26.111314284099596 27.88120520646934 23.441958126318777 86-87 22.3302272953777 26.21917070666792 28.11005105796463 23.340550939989747 88-89 22.888668588216994 25.623742127786393 27.937134675650615 23.550454608345994 90-91 23.415238302266182 25.484517145807295 27.787294168795174 23.31295038313135 92-93 23.658081703896116 25.423600780264742 27.65418320613508 23.264134309704062 94-95 23.415037322331163 25.179796528785165 27.81450072075131 23.590665428132365 96-97 23.699917815690842 24.976214810936415 27.612395276418518 23.711472096954225 98-99 23.858357227503735 25.010658079041313 27.393513235475393 23.73747145797956 100 24.483878226066803 24.758409812525883 27.19201884415419 23.56569311725312 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3532.0 1 2318.5 2 2637.5 3 3906.5 4 2645.5 5 1799.0 6 2429.0 7 3773.5 8 5573.5 9 6461.0 10 5465.0 11 4463.5 12 4513.0 13 4906.0 14 5425.5 15 5898.5 16 6445.5 17 6892.0 18 7476.5 19 8241.0 20 8931.0 21 9940.5 22 11309.5 23 13118.0 24 15384.0 25 18508.0 26 22979.5 27 28109.5 28 33589.5 29 40998.0 30 50700.5 31 60272.5 32 71553.5 33 83363.0 34 94543.0 35 104308.0 36 114084.0 37 127555.5 38 135583.0 39 136286.0 40 136446.5 41 137765.0 42 140978.5 43 146981.5 44 156130.0 45 167537.0 46 177010.0 47 184743.5 48 191628.5 49 193905.0 50 191421.0 51 188354.0 52 182226.5 53 172456.5 54 161604.0 55 145724.0 56 128883.0 57 110528.5 58 91549.0 59 75936.5 60 60339.5 61 46373.5 62 35702.5 63 27206.5 64 19953.0 65 13964.0 66 9808.5 67 7461.5 68 5783.5 69 4419.0 70 3474.0 71 2677.0 72 2073.0 73 1556.0 74 1182.0 75 897.0 76 630.0 77 420.0 78 302.0 79 226.5 80 155.0 81 121.0 82 100.5 83 81.0 84 64.0 85 46.0 86 33.5 87 23.5 88 20.5 89 19.5 90 13.5 91 10.5 92 12.0 93 11.5 94 9.5 95 6.5 96 3.5 97 1.5 98 1.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22020591382846197 2 0.15007124308976805 3 0.02421134899377286 4 0.02310292996443496 5 0.02807994874322669 6 0.05531228293460676 7 0.06263654201081992 8 0.05539921776043717 9 0.04270673318919539 10-11 0.04481490271558315 12-13 0.04298927137314427 14-15 0.020505752042751072 16-17 0.018767055526142606 18-19 0.018147644892100842 20-21 0.02180977443020742 22-23 0.02476555850844181 24-25 0.022342250238418758 26-27 0.020147145886200573 28-29 0.01863665328739697 30-31 0.017147894395050975 32-33 0.025493637674771603 34-35 0.025993512923296536 36-37 0.02443955291157772 38-39 0.023450669267756657 40-41 0.02433088437928969 42-43 0.030296786801902485 44-45 0.040468161424061996 46-47 0.045466913909311325 48-49 0.041413577654967844 50-51 0.02892756329507332 52-53 0.04486923698172717 54-55 0.04304360563928829 56-57 0.045369112230252105 58-59 0.048379230574630505 60-61 0.024635156269696173 62-63 0.02575444215226287 64-65 0.026536855584736677 66-67 0.032024616465282145 68-69 0.036719097060124994 70-71 0.041076705204874955 72-73 0.04418462522831258 74-75 0.025384969142483574 76-77 0.01840844936959211 78-79 0.01758256852420309 80-81 0.02107082841064882 82-83 0.018984392590718664 84-85 0.011421062743471848 86-87 0.012877221076131436 88-89 0.01889745776488824 90-91 0.019516868398930007 92-93 0.019875474555480502 94-95 0.0236245389194175 96-97 0.0243743517922049 98-99 0.029362237424225432 100 0.028536356578836415 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4601148.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.02945396877345 #Duplication Level Percentage of deduplicated Percentage of total 1 80.5106644230877 54.77096539365811 2 12.738830628013591 17.332313836489043 3 3.35032060876466 6.8376144490376545 4 1.2853890061029727 3.4977724889059854 5 0.6306462768640873 2.1451260931251888 6 0.3608259904538397 1.4728077064989946 7 0.24965587225250557 1.1888766868605067 8 0.16601918792072293 0.9035355762068774 9 0.12128630512242317 0.7425937000231648 >10 0.5474585853555519 6.242012461833559 >50 0.025496427713111797 1.1806107029284374 >100 0.012302504203135512 1.4668377964889825 >500 6.411392214893773E-4 0.2974877385651114 >1k 2.849507225627229E-4 0.41367679764465354 >5k 7.123768064067064E-5 0.28055002127046924 >10k+ 1.0685652096100595E-4 1.2272185504633084 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22389 0.4865959538793362 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17911 0.3892724163621774 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10346 0.2248569270103896 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5790 0.12583816038953755 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5788 0.12579469297662238 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 2.1733706457605797E-5 0.0 8 0.0 0.0 0.0 2.1733706457605797E-5 2.1733706457605797E-5 9 0.0 0.0 0.0 2.1733706457605797E-5 4.3467412915211594E-5 10-11 0.0 0.0 0.0 3.2600559686408696E-5 4.3467412915211594E-5 12-13 0.0 0.0 0.0 5.433426614401449E-5 8.693482583042319E-5 14-15 0.0 0.0 0.0 7.606797260162028E-5 1.6300279843204347E-4 16-17 0.0 0.0 0.0 1.630027984320435E-4 2.3907077103366378E-4 18-19 0.0 0.0 0.0 3.26005596864087E-4 2.825381839488754E-4 20-21 0.0 0.0 0.0 0.001097552176109093 3.26005596864087E-4 22-23 0.0 0.0 0.0 0.004248939612461933 3.6947300977929856E-4 24-25 0.0 0.0 0.0 0.011095057146607759 3.6947300977929856E-4 26-27 0.0 0.0 0.0 0.02305946255151975 3.912067162369044E-4 28-29 0.0 0.0 0.0 0.0577029906449434 3.912067162369044E-4 30-31 0.0 0.0 0.0 0.1597318756101738 3.912067162369044E-4 32-33 0.0 0.0 0.0 0.3757649177987754 3.912067162369044E-4 34-35 0.0 0.0 0.0 0.702400792150133 4.3467412915211594E-4 36-37 0.0 0.0 0.0 1.1904855049218153 4.3467412915211594E-4 38-39 0.0 0.0 0.0 1.8383564275698152 5.107421017537363E-4 40-41 0.0 0.0 0.0 2.4980613533839815 5.216089549825391E-4 42-43 0.0 0.0 0.0 3.1392056938833526 5.542095146689478E-4 44-45 0.0 0.0 0.0 3.8906811952147597 5.759432211265537E-4 46-47 0.0 0.0 0.0 4.743479236051525 5.976769275841594E-4 48-49 0.0 0.0 0.0 5.611403936582784 6.302774872705681E-4 50-51 0.0 0.0 0.0 6.45892068675035 6.302774872705681E-4 52-53 0.0 0.0 0.0 7.25276604882086 6.737449001857798E-4 54-55 0.0 0.0 0.0 8.112757946495092 6.737449001857798E-4 56-57 0.0 0.0 0.0 9.06737840208574 6.954786066433855E-4 58-59 0.0 0.0 0.0 10.083374844712667 7.172123131009913E-4 60-61 0.0 0.0 0.0 11.082821069872129 7.280791663297942E-4 62-63 0.0 0.0 0.0 12.070313756479905 8.041471389314145E-4 64-65 0.0 0.0 0.0 13.035442459142805 8.041471389314145E-4 66-67 0.0 0.0 0.0 14.069456144423087 8.041471389314145E-4 68-69 0.0 0.0 0.0 15.159792729988254 8.041471389314145E-4 70-71 0.0 0.0 0.0 16.254671660203062 8.584814050754291E-4 72-73 0.0 0.0 0.0 17.282328236344497 9.888836438210638E-4 74-75 0.0 0.0 0.0 18.279155549875814 0.0010540847631938812 76-77 0.0 0.0 0.0 19.33446826748455 0.0010649516164226841 78-79 0.0 0.0 0.0 20.434824091726675 0.0010649516164226841 80-81 2.1733706457605797E-5 0.0 0.0 21.534212766031434 0.001075818469651487 82-83 2.1733706457605797E-5 0.0 0.0 22.615355993765036 0.0012388212680835304 84-85 2.1733706457605797E-5 0.0 0.0 23.633493206478036 0.0012388212680835304 86-87 2.1733706457605797E-5 0.0 0.0 24.658400468752582 0.0012822886809987421 88 2.1733706457605797E-5 0.0 0.0 25.433587443829236 0.0013040223874563478 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8365 0.0 57.450905 1 GTATCAA 15960 0.0 43.897594 1 TACCTGG 3645 0.0 41.06449 2 ATCAACG 19650 0.0 35.021076 3 TATCAAC 19865 0.0 34.94945 2 CAACGCA 19805 0.0 34.74699 5 TATCACG 245 0.0 34.58146 2 TCAACGC 20830 0.0 33.104862 4 AACGCAG 21050 0.0 32.959846 6 ACCTGGG 4485 0.0 32.804455 3 ACGCAGA 23170 0.0 29.700974 7 TAACGCA 415 0.0 29.449383 4 CGCAGAG 23460 0.0 29.212654 8 GTACTAG 1730 0.0 27.23432 1 GTACATG 15325 0.0 26.562927 1 GCAGAGT 25175 0.0 26.41916 9 GTGGTAT 3250 0.0 26.38461 1 TACATGG 15535 0.0 25.481302 2 CATGGGG 10720 0.0 24.642939 4 ACATGGG 15405 0.0 24.28859 3 >>END_MODULE