FastQCFastQC Report
Mon 6 Jun 2016
ERR1211458_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211458_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4601148
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198760.4319791495513728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171860.3735154791804132No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101170.21987990823159786No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59550.12942422195504252No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47530.10330030679300037No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86750.056.0277821
GTATCAA159600.041.6161231
TACCTGG32950.038.2203642
GTGGTAT32200.033.575641
ATCAACG195550.033.3306273
CAACGCA197650.032.9246525
TATCAAC203300.032.452282
TCAACGC205850.031.6635724
AACGCAG210550.031.2191646
ACCTGGG39700.031.012423
ACGCAGA234450.027.7160117
TGGTATC40000.027.607262
CGCAGAG238050.027.2376568
GTACATG152700.024.7188851
GCAGAGT257650.024.6365159
TACATGG155700.023.6917152
ACATGGG155650.023.0960243
GTACTAG17150.022.4751241
CATGGGG110350.022.1018874
GAGTACT173400.022.06872412-13
Produced by FastQC (version 0.11.5)