##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211458_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4601148 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04699001205786 33.0 31.0 34.0 30.0 34.0 2 32.175860241835295 33.0 31.0 34.0 30.0 34.0 3 32.255252602176675 34.0 31.0 34.0 30.0 34.0 4 35.902923140051136 37.0 35.0 37.0 35.0 37.0 5 35.76171924919607 37.0 35.0 37.0 35.0 37.0 6 35.80385003916414 37.0 35.0 37.0 35.0 37.0 7 35.73747008355306 37.0 35.0 37.0 33.0 37.0 8 35.72576909067042 37.0 35.0 37.0 33.0 37.0 9 37.307732113811596 39.0 37.0 39.0 34.0 39.0 10-11 37.28760604962066 39.0 37.0 39.0 34.0 39.0 12-13 37.20627047858491 39.0 37.0 39.0 33.0 39.0 14-15 38.400997859664585 40.0 38.0 41.0 33.5 41.0 16-17 38.394203033677684 40.0 38.0 41.0 33.5 41.0 18-19 38.41305180794011 40.0 38.0 41.0 33.5 41.0 20-21 38.37851912174962 40.0 38.0 41.0 34.0 41.0 22-23 38.267289598161156 40.0 38.0 41.0 34.0 41.0 24-25 38.1467824986286 40.0 37.0 41.0 33.0 41.0 26-27 38.011799446572894 40.0 37.0 41.0 33.0 41.0 28-29 37.93095766534786 40.0 37.0 41.0 33.0 41.0 30-31 37.95267474552003 40.0 37.0 41.0 33.0 41.0 32-33 37.76973398812644 40.0 37.0 41.0 33.0 41.0 34-35 37.64233599962444 40.0 36.5 41.0 33.0 41.0 36-37 37.48210740015318 40.0 36.0 41.0 32.0 41.0 38-39 37.354074461417014 40.0 36.0 41.0 32.0 41.0 40-41 37.26945253662782 40.0 36.0 41.0 32.0 41.0 42-43 37.11468996433064 39.0 36.0 41.0 31.0 41.0 44-45 36.93964191110567 39.0 35.5 41.0 31.0 41.0 46-47 36.697269681392555 39.0 35.0 41.0 30.0 41.0 48-49 36.454922010767746 39.0 35.0 41.0 30.0 41.0 50-51 36.26452843942425 39.0 35.0 41.0 29.0 41.0 52-53 36.056694220659715 39.0 35.0 40.0 28.5 41.0 54-55 35.85479449911196 38.5 35.0 40.0 28.0 41.0 56-57 35.62318534417932 38.0 34.5 40.0 27.5 41.0 58-59 35.36506378408171 38.0 34.0 40.0 27.0 41.0 60-61 35.11203106268262 38.0 34.0 40.0 26.5 41.0 62-63 34.825918553369725 37.0 34.0 40.0 26.0 41.0 64-65 34.47923300880563 36.5 33.5 39.5 26.0 41.0 66-67 34.1329219360038 36.0 33.0 39.0 25.5 41.0 68-69 33.765649137997734 35.5 33.0 39.0 25.0 40.0 70-71 33.398993033912404 35.0 32.5 38.5 24.5 40.0 72-73 33.05144813859498 35.0 32.0 37.5 24.0 40.0 74-75 32.69258030821873 35.0 32.0 37.0 23.5 39.0 76-77 31.667850066983284 34.0 30.5 36.0 22.5 38.5 78-79 32.140541556150765 35.0 31.5 36.0 23.5 38.5 80-81 32.03106757270142 35.0 31.5 36.0 23.5 37.5 82-83 31.76624920563303 34.5 31.0 35.0 23.0 37.0 84-85 31.49210077571945 34.0 31.0 35.0 22.5 36.5 86-87 31.238899400758243 34.0 31.0 35.0 20.5 36.0 88-89 31.008085590813423 34.0 31.0 35.0 20.0 36.0 90-91 30.79409464768358 34.0 30.5 35.0 20.0 35.0 92-93 30.62207909852063 34.0 30.0 35.0 19.5 35.0 94-95 30.46043237470301 34.0 30.0 35.0 19.0 35.0 96-97 30.29719474357269 34.0 30.0 35.0 19.0 35.0 98-99 30.103917652724927 34.0 29.0 35.0 18.0 35.0 100 28.28022397888527 32.0 25.0 34.0 12.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.18544501884245435 1101 2 -0.14892755415958447 1101 3 -0.2446287052993732 1101 4 -0.10356915455829352 1101 5 -0.1123377766284932 1101 6 -0.24979417965678152 1101 7 -0.31068288607462335 1101 8 -0.23435417455223018 1101 9 -0.42453759006073 1101 10-11 -0.40649176305590373 1101 12-13 -0.41666987379964837 1101 14-15 -0.6357671706972496 1101 16-17 -0.6081505345147775 1101 18-19 -0.614991729706297 1101 20-21 -0.6381531347634208 1101 22-23 -0.665872295165272 1101 24-25 -0.7062314957937517 1101 26-27 -0.7634323153920093 1101 28-29 -0.7068349883648963 1101 30-31 -0.6332153427245899 1101 32-33 -0.48817354590141804 1101 34-35 -0.5941158338306138 1101 36-37 -0.5987825978879044 1101 38-39 -0.611677295335042 1101 40-41 -0.582964499185934 1101 42-43 -0.5609179921400553 1101 44-45 -0.5557544044944152 1101 46-47 -0.623032926453682 1101 48-49 -0.6404561406326508 1101 50-51 -0.7111760112838823 1101 52-53 -0.7387289280388885 1101 54-55 -0.6533589773589128 1101 56-57 -0.7059806647708342 1101 58-59 -0.7158490363735837 1101 60-61 -0.6691787159799816 1101 62-63 -0.6655066552490183 1101 64-65 -0.6847606741340186 1101 66-67 -0.7171620380470713 1101 68-69 -0.7954241406137399 1101 70-71 -0.7994609495660825 1101 72-73 -0.7996380677054447 1101 74-75 -0.8580626056326182 1101 76-77 -0.6647889616249962 1101 78-79 -0.7111660500011148 1101 80-81 -0.5022733524287304 1101 82-83 -0.5958329796935935 1101 84-85 -0.6003433165767582 1101 86-87 -0.7025798742100378 1101 88-89 -0.7590628326083966 1101 90-91 -0.7639087497355774 1101 92-93 -0.7884112828291912 1101 94-95 -0.9395658856728737 1101 96-97 -1.000552896563228 1101 98-99 -0.9363028588652895 1101 100 -1.074568155574731 1102 1 -0.32625532470759566 1102 2 -0.2767726519245244 1102 3 -0.36874240335465913 1102 4 -0.19227389668719752 1102 5 -0.25054795234830607 1102 6 -0.2827561175839861 1102 7 -0.37146917601609886 1102 8 -0.36635391605734213 1102 9 -0.5731210546105459 1102 10-11 -0.48839970225147766 1102 12-13 -0.5187074822068283 1102 14-15 -0.6898594413220209 1102 16-17 -0.7405296598002096 1102 18-19 -0.7346274586618833 1102 20-21 -0.7297467758364107 1102 22-23 -0.7188231655416644 1102 24-25 -0.7778182172846542 1102 26-27 -0.8129280908672243 1102 28-29 -0.7504884308363202 1102 30-31 -0.731766459970693 1102 32-33 -0.6883129476582326 1102 34-35 -0.6753143297873194 1102 36-37 -0.7002304114559053 1102 38-39 -0.6866380071836531 1102 40-41 -0.7193551981428783 1102 42-43 -0.7562434877697441 1102 44-45 -0.788345075002745 1102 46-47 -0.8108279863186425 1102 48-49 -0.8619021135469254 1102 50-51 -0.7956893084121788 1102 52-53 -0.7320568536150418 1102 54-55 -0.8994985369535655 1102 56-57 -0.8195499451815493 1102 58-59 -0.7880386074043884 1102 60-61 -0.9565984207098239 1102 62-63 -0.961672300616712 1102 64-65 -0.9516994141533743 1102 66-67 -0.9404119815751528 1102 68-69 -1.0333657025750114 1102 70-71 -0.9933278334409152 1102 72-73 -1.0747226160907672 1102 74-75 -1.0014466368863992 1102 76-77 -0.8359559951622266 1102 78-79 -0.760655416268051 1102 80-81 -0.6275814079233761 1102 82-83 -0.5510534068386761 1102 84-85 -0.7099870012205542 1102 86-87 -0.8344349211927806 1102 88-89 -0.672203115468907 1102 90-91 -0.641541605239297 1102 92-93 -0.7748027319883981 1102 94-95 -0.7840330967540332 1102 96-97 -0.9147638420968889 1102 98-99 -1.0720560926505236 1102 100 -0.9505886669314947 1103 1 -0.2783614136442054 1103 2 -0.2248667351591216 1103 3 -0.32751480412480305 1103 4 -0.17593938469075 1103 5 -0.15874182083172172 1103 6 -0.267483096627231 1103 7 -0.32726213550958505 1103 8 -0.3839739267949014 1103 9 -0.4684916392521359 1103 10-11 -0.37656952693305357 1103 12-13 -0.4683555031635862 1103 14-15 -0.6997040938323877 1103 16-17 -0.8161557474409094 1103 18-19 -0.6741730331212992 1103 20-21 -0.6031327864628864 1103 22-23 -0.7715457384830131 1103 24-25 -0.7254797897816943 1103 26-27 -0.7683034657561407 1103 28-29 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-0.9331441987224096 1104 50-51 -0.9912274410322865 1104 52-53 -0.8809076597624355 1104 54-55 -0.8241232724187668 1104 56-57 -0.8677994887293536 1104 58-59 -0.9100351825982855 1104 60-61 -1.0234700232531324 1104 62-63 -1.0448745650534335 1104 64-65 -0.9257042729172511 1104 66-67 -1.0140163719096833 1104 68-69 -1.030488297241689 1104 70-71 -0.9849359857337419 1104 72-73 -0.9695212890786067 1104 74-75 -0.9681236224534544 1104 76-77 -0.7422883970438683 1104 78-79 -0.7400143932870051 1104 80-81 -0.8899672712970492 1104 82-83 -0.8573221034601772 1104 84-85 -0.7876082180428909 1104 86-87 -0.7434714368969964 1104 88-89 -0.8549787409215845 1104 90-91 -0.8418476121124883 1104 92-93 -0.8907783702670073 1104 94-95 -1.0320728396884569 1104 96-97 -1.1635308788922174 1104 98-99 -1.1809561324486282 1104 100 -1.3579302855637962 1105 1 -0.40870485105842036 1105 2 -0.435716730281392 1105 3 -0.45701121842765957 1105 4 -0.2583952185666831 1105 5 -0.3090444668289223 1105 6 -0.374756696322315 1105 7 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0.8972218550507094 2311 36-37 0.9331788917922239 2311 38-39 1.0309632312406691 2311 40-41 0.9663249471094986 2311 42-43 1.0980608297709509 2311 44-45 1.1290084460415812 2311 46-47 1.091835955511037 2311 48-49 1.0815987510161804 2311 50-51 1.110603142972856 2311 52-53 1.0364553824999234 2311 54-55 1.1266145293298493 2311 56-57 1.1699947302947749 2311 58-59 1.0940615673869303 2311 60-61 1.0964076883154519 2311 62-63 1.1230879863474286 2311 64-65 1.1137920536601627 2311 66-67 1.2079019126367143 2311 68-69 1.2143737611718706 2311 70-71 1.2361689153249245 2311 72-73 1.1694605957774087 2311 74-75 1.1490799228678057 2311 76-77 1.0381462935274293 2311 78-79 1.030806580529486 2311 80-81 0.9618444217392685 2311 82-83 1.0088354716932812 2311 84-85 1.0114767713093897 2311 86-87 1.0415389768053984 2311 88-89 1.0900731025064907 2311 90-91 1.1701072896517957 2311 92-93 1.1708891208140422 2311 94-95 1.0908217104769378 2311 96-97 1.2312417354731693 2311 98-99 1.190861141238905 2311 100 1.3051908633358025 2312 1 0.07345839359827977 2312 2 0.12676395348798764 2312 3 0.33391864834799634 2312 4 0.23667802716616393 2312 5 0.3356130503279573 2312 6 0.37821528633005386 2312 7 0.4503196132338516 2312 8 0.2933121601296733 2312 9 0.36212580409538475 2312 10-11 0.22401151852013612 2312 12-13 0.37401366648157364 2312 14-15 0.72119906555033 2312 16-17 0.8002054603288755 2312 18-19 0.779827892352678 2312 20-21 0.774626621793594 2312 22-23 0.36628025491445726 2312 24-25 0.33008481932364475 2312 26-27 0.6465478548374861 2312 28-29 0.7932437430228063 2312 30-31 0.7411000447646785 2312 32-33 0.5417154209596902 2312 34-35 0.7648149358694027 2312 36-37 0.870465873280736 2312 38-39 0.9085042432575818 2312 40-41 0.8142991228848331 2312 42-43 0.6767976338387811 2312 44-45 0.6768008992814671 2312 46-47 0.8268513745790642 2312 48-49 0.9426944983687946 2312 50-51 0.9984476035594838 2312 52-53 0.9310029484913613 2312 54-55 0.9275680246668472 2312 56-57 0.8723418824070421 2312 58-59 1.0042416552184648 2312 60-61 0.9986496294095701 2312 62-63 1.066624535090071 2312 64-65 1.0951380950751215 2312 66-67 1.0652241160536988 2312 68-69 1.055034787905214 2312 70-71 0.9404735126940764 2312 72-73 0.9818136926399674 2312 74-75 1.0131288918776704 2312 76-77 1.0103490549888896 2312 78-79 0.9366039001886719 2312 80-81 0.7786266853248272 2312 82-83 0.800817761171885 2312 84-85 0.6745670622743916 2312 86-87 0.8720865534490372 2312 88-89 0.44142023782105255 2312 90-91 0.7687035952209733 2312 92-93 1.0645428233364456 2312 94-95 1.0843156708810504 2312 96-97 1.1682379322259706 2312 98-99 1.1551472652248442 2312 100 1.2879387965005833 2313 1 -0.4826018454198042 2313 2 -0.10800902547103419 2313 3 0.14958517658720183 2313 4 0.017403156365126904 2313 5 0.20321294881448182 2313 6 0.28207741257589447 2313 7 0.06114233379494749 2313 8 0.028353342300214024 2313 9 0.182562312031898 2313 10-11 0.3129237670435785 2313 12-13 0.5068477957728206 2313 14-15 0.5189233757201279 2313 16-17 0.6844368418349589 2313 18-19 0.5569019971884117 2313 20-21 0.7254704525429503 2313 22-23 0.7894832933972893 2313 24-25 0.6094285577880214 2313 26-27 0.7669768646196999 2313 28-29 0.7115902846433286 2313 30-31 0.7475383637938435 2313 32-33 0.899478317785082 2313 34-35 0.8789728931414587 2313 36-37 0.7444319931589263 2313 38-39 0.5267948371114031 2313 40-41 0.59256283873939 2313 42-43 0.6354943392651435 2313 44-45 0.8719389229987087 2313 46-47 0.8903722771261258 2313 48-49 0.8865563939022039 2313 50-51 0.7844228827215431 2313 52-53 0.8021341892628655 2313 54-55 0.8346976158443979 2313 56-57 0.8602511085975237 2313 58-59 0.8016660392162507 2313 60-61 0.8037264566520932 2313 62-63 0.9277745789254368 2313 64-65 1.0047867777070891 2313 66-67 1.0278241875745522 2313 68-69 1.006869240193879 2313 70-71 0.956075441450075 2313 72-73 0.9965749629426597 2313 74-75 0.7231002835351177 2313 76-77 0.8405576767127734 2313 78-79 0.69273075742381 2313 80-81 0.8074715081376773 2313 82-83 0.8684539968677143 2313 84-85 0.8657959520861311 2313 86-87 0.8894505484656463 2313 88-89 0.8623853656357454 2313 90-91 0.9995659983217493 2313 92-93 0.7925573697557873 2313 94-95 0.8041442515381156 2313 96-97 1.105851846493227 2313 98-99 1.16427771003843 2313 100 1.1793395670801345 2314 1 -0.36060683442043384 2314 2 -0.09306664122632924 2314 3 0.13491293166786278 2314 4 -0.16659734122548286 2314 5 0.19637068503907784 2314 6 0.20044269646443524 2314 7 0.11924441843773081 2314 8 0.16882441657216418 2314 9 0.27306252154373567 2314 10-11 0.43310064086507083 2314 12-13 0.4837637487897908 2314 14-15 0.5639249143226834 2314 16-17 0.8041439835697233 2314 18-19 0.8342320307103037 2314 20-21 0.4337627346506352 2314 22-23 0.6942204542500008 2314 24-25 0.8906841229462046 2314 26-27 0.8767847029813112 2314 28-29 0.8143677241467842 2314 30-31 0.8135966277263762 2314 32-33 0.9032946413761138 2314 34-35 0.9183807275747284 2314 36-37 0.8463591419646974 2314 38-39 0.8716310577797444 2314 40-41 0.9063049493092592 2314 42-43 0.7179080465771008 2314 44-45 0.7372560388063718 2314 46-47 0.8626039710293298 2314 48-49 0.72258994198517 2314 50-51 0.9273770978378195 2314 52-53 0.9793210672745971 2314 54-55 1.0397302865965443 2314 56-57 0.8862654405166523 2314 58-59 0.8965423944959525 2314 60-61 0.9117522004191159 2314 62-63 0.6823622661766393 2314 64-65 0.8331964191799628 2314 66-67 0.9863992779265374 2314 68-69 0.9781426465944705 2314 70-71 1.0266315644335222 2314 72-73 0.9952171625550648 2314 74-75 1.045567809200307 2314 76-77 0.870080480766827 2314 78-79 0.8487919021441144 2314 80-81 0.9291265207083832 2314 82-83 0.8573076397547936 2314 84-85 0.7867881870536593 2314 86-87 0.8022382998798534 2314 88-89 0.9453317240581178 2314 90-91 0.9817897045861521 2314 92-93 1.0748150889575463 2314 94-95 1.1384799680686015 2314 96-97 1.1737182630551395 2314 98-99 1.242217292062314 2314 100 1.2102971016495871 2315 1 -0.002768996582595662 2315 2 0.15789815617019087 2315 3 0.1293067087048101 2315 4 -0.054696718135431865 2315 5 0.28589959721116287 2315 6 0.3797505505740517 2315 7 0.4702393781551564 2315 8 0.4318088988517985 2315 9 0.6670872070221634 2315 10-11 0.6002360141238015 2315 12-13 0.6699394839963659 2315 14-15 0.8312551520494011 2315 16-17 0.7759846788905946 2315 18-19 0.7929834693736311 2315 20-21 0.834257237964529 2315 22-23 0.915351144411531 2315 24-25 0.8340353155484976 2315 26-27 0.8947574775179632 2315 28-29 0.928990893571715 2315 30-31 0.9288373309846989 2315 32-33 0.9722187196394003 2315 34-35 0.9799324996110812 2315 36-37 1.0661805492750958 2315 38-39 0.9837257515925018 2315 40-41 0.9919516532819586 2315 42-43 1.032779261404265 2315 44-45 0.8896872806208833 2315 46-47 0.904168442990283 2315 48-49 0.9852200169940986 2315 50-51 0.858566525150593 2315 52-53 0.9171704144190969 2315 54-55 0.8129767048209331 2315 56-57 0.9033976387942175 2315 58-59 0.903330107318908 2315 60-61 0.9784605387133567 2315 62-63 0.9256694059154995 2315 64-65 0.9919776232915609 2315 66-67 1.1068136129664197 2315 68-69 1.0087597835552202 2315 70-71 0.6443201781573791 2315 72-73 0.7891525516886944 2315 74-75 0.930508079867451 2315 76-77 0.8840881007215806 2315 78-79 0.7381275373387766 2315 80-81 0.7158058872996413 2315 82-83 0.7511739730484379 2315 84-85 0.7055170039763112 2315 86-87 0.7069269532909743 2315 88-89 0.8876439040795496 2315 90-91 0.9622643642678597 2315 92-93 0.9426136364036743 2315 94-95 1.003300780070031 2315 96-97 1.1007000466007995 2315 98-99 1.044555665678221 2315 100 0.925691791493616 2316 1 0.4262287215146472 2316 2 0.4300832385112301 2316 3 0.4393976059155733 2316 4 0.26334444890587605 2316 5 0.3611370627912436 2316 6 0.34830591513334497 2316 7 0.40502678848550033 2316 8 0.42015982781278893 2316 9 0.4785506637143371 2316 10-11 0.435449957005865 2316 12-13 0.610611497011952 2316 14-15 0.7589539718161973 2316 16-17 0.7244533450639139 2316 18-19 0.7800440987024686 2316 20-21 0.8004919282992589 2316 22-23 0.8186947389612556 2316 24-25 0.8921742386543272 2316 26-27 0.9212787705085574 2316 28-29 0.9120907552347504 2316 30-31 0.7441224141763385 2316 32-33 0.6711959075712173 2316 34-35 0.5925032861522652 2316 36-37 0.6754741337337222 2316 38-39 0.6257573641809344 2316 40-41 0.6868548831293424 2316 42-43 0.8151844224790707 2316 44-45 0.7632296427420684 2316 46-47 0.8737349123972393 2316 48-49 0.6464980681207848 2316 50-51 0.7437260534284817 2316 52-53 0.8851211327429098 2316 54-55 0.9738106702977873 2316 56-57 0.970163082234194 2316 58-59 0.8959750499001728 2316 60-61 0.9734545188512911 2316 62-63 0.9223621238542279 2316 64-65 0.9288695804929361 2316 66-67 0.9627182509279777 2316 68-69 0.9678861341578653 2316 70-71 0.8583588367295079 2316 72-73 0.8155208311525683 2316 74-75 0.91919926055688 2316 76-77 0.7919102252032069 2316 78-79 0.782327475632286 2316 80-81 0.7419326918083904 2316 82-83 0.7178105597363853 2316 84-85 0.7067089134672742 2316 86-87 0.6553250359049798 2316 88-89 0.6151556712280382 2316 90-91 0.6792521907690201 2316 92-93 0.7813915232117061 2316 94-95 0.6818686173814221 2316 96-97 0.8037844429791363 2316 98-99 0.8588537389357285 2316 100 0.5957492836894467 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 6.0 12 23.0 13 172.0 14 778.0 15 2218.0 16 5120.0 17 10332.0 18 16179.0 19 22285.0 20 29268.0 21 37567.0 22 42861.0 23 42552.0 24 41976.0 25 44328.0 26 50613.0 27 61338.0 28 74456.0 29 91056.0 30 111047.0 31 136936.0 32 172127.0 33 223071.0 34 307497.0 35 428269.0 36 638902.0 37 975547.0 38 900904.0 39 133706.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.98369274613911 20.216532706380494 13.106471735477744 23.69330281200265 2 15.869756851985633 21.679002718452004 39.410316729650944 23.040923699911414 3 18.49505818982567 27.033470777292973 30.94047398605739 23.530997046823966 4 12.430821293086433 17.825499673506936 38.82176434939193 30.921914684014705 5 12.442156231804113 37.913975115252846 35.161164893236666 14.482703759706375 6 28.81714059559497 38.09261038606243 18.836303208743384 14.253945809599214 7 25.634277592075005 33.620472317729956 23.2011414562478 17.54410863394724 8 25.383650237300348 35.05718485302853 21.207198838720306 18.351966070950816 9 26.54190287994005 15.72969580106184 21.229918792767243 36.498482526230866 10-11 24.30928653768915 26.371831418144136 29.05601950137271 20.262862542794004 12-13 24.898704341428797 24.75704364249256 28.65951553258055 21.684736483498092 14-15 22.42648896600977 26.058105958819826 26.899505036353986 24.615900038816417 16-17 21.33829108586908 28.590324493212993 27.78125396640488 22.29013045451305 18-19 20.89270981937551 27.797769165434367 30.19706168982176 21.112459325368366 20-21 21.82311043060454 27.285693405829743 29.853683238800755 21.037512924764965 22-23 21.82455335059859 27.177097976418057 29.544452819166 21.45389585381735 24-25 21.349223149721023 27.135240714048507 30.00549428576893 21.510041850461537 26-27 21.313085485740242 27.260460569890444 29.749165833088377 21.677288111280934 28-29 21.09379567524875 27.844207587886103 29.701590729341287 21.360406007523856 30-31 21.766126321653058 27.390690081194652 29.704004702350208 21.139178894802082 32-33 21.640709891658204 27.517463737353907 29.400136897181262 21.441689473806623 34-35 21.679700173723333 27.49510041073835 29.724678462926214 21.100520952612108 36-37 21.63699254125831 27.23508705488676 29.5979766816627 21.529943722192225 38-39 21.45028076334937 27.240571741413167 29.823140230160405 21.486007265077056 40-41 21.446337970911365 26.682404779941166 30.26084053652303 21.610416712624435 42-43 21.237108158995223 27.01172271052662 29.693751604246753 22.057417526231404 44-45 20.823462567818382 27.400375637918263 29.93897459673046 21.837187197532902 46-47 20.81246647781028 27.03178297564557 29.60514596519181 22.550604581352342 48-49 20.917876758627134 27.023467198737634 29.576679729356712 22.48197631327852 50-51 20.85036027877223 27.148546837761906 29.32092442552161 22.680168457944255 52-53 20.532632266299395 27.450061037958307 29.127463690240184 22.88984300550211 54-55 20.39455373175967 27.13274732722244 29.348218631916527 23.12448030910136 56-57 20.743004011183196 26.996596291924067 28.879877826864806 23.38052187002793 58-59 20.344425930699668 27.17518267530809 29.045852165260456 23.434539228731786 60-61 20.652077409225377 27.316122227403373 28.618338370375906 23.41346199299534 62-63 20.665384003073175 26.988922763620245 28.525000258090316 23.820692975216264 64-65 20.211032600266353 26.822351965405616 28.49536805821466 24.471247376113368 66-67 20.365824782179594 26.7014627033583 28.266284155964 24.66642835849811 68-69 20.09186004689227 26.75032716293954 28.293983898668863 24.86382889149933 70-71 20.009837064379933 26.52593547242371 28.27929869819804 25.184928764998315 72-73 19.896408543224076 26.60112070975748 28.121944179761453 25.38052656725699 74-75 19.778001842665578 26.585277197687407 28.002810086746155 25.633910872900863 76-77 19.599040619325553 26.452731805491165 28.13868083526773 25.80954673991555 78-79 19.386229123071743 26.4875093276019 28.111213054491017 26.01504849483534 80-81 19.252727419729226 26.480172360581882 28.008344613362336 26.258755606326556 82-83 19.45347995811652 25.97577841018221 28.317305283185775 26.253436348515496 84-85 19.557300681370254 26.081394782481638 27.93611523280875 26.42518930333936 86-87 19.071535599499622 26.226237120093447 28.152392938613723 26.549834341793204 88-89 19.244413525665728 25.67158820362089 28.15464328244348 26.929354988269903 90-91 19.36092377200379 25.369596526984605 28.15054820807959 27.118931492932013 92-93 19.280772163068338 25.29931232025663 28.25929393749208 27.16062157918295 94-95 18.74073601532946 25.01713555284613 28.68358417841015 27.558544253414258 96-97 18.8249635024122 24.942822075862388 28.6524592864708 27.579755135254615 98-99 18.727486533902546 25.12942203032566 28.429109095796267 27.713982339975523 100 18.66413756119426 24.962518822105213 28.57134162074765 27.802001995952878 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3414.0 1 2274.5 2 2295.5 3 3334.5 4 2306.5 5 1562.5 6 2235.0 7 3545.5 8 5074.5 9 5793.5 10 4890.0 11 3914.0 12 3976.5 13 4245.0 14 4760.5 15 5360.5 16 5830.5 17 6422.0 18 7161.5 19 7891.0 20 8609.5 21 9645.5 22 11056.5 23 12903.5 24 15164.5 25 18264.5 26 22806.0 27 28074.5 28 33651.5 29 41294.5 30 50995.0 31 60313.5 32 71633.0 33 83348.0 34 94445.5 35 105067.5 36 115108.5 37 127765.0 38 135892.5 39 137087.5 40 137243.5 41 139730.5 42 145009.5 43 151528.0 44 160051.0 45 172333.5 46 181383.5 47 188251.5 48 196488.0 49 198817.0 50 196211.0 51 192452.5 52 184369.5 53 171533.0 54 158963.0 55 142804.5 56 124795.0 57 105812.5 58 87152.5 59 72748.5 60 57338.5 61 43366.0 62 33401.0 63 25361.5 64 18329.0 65 12628.0 66 8844.5 67 6724.0 68 5154.0 69 3743.0 70 2871.5 71 2301.5 72 1782.5 73 1276.0 74 889.5 75 610.0 76 413.5 77 261.5 78 165.0 79 107.0 80 66.0 81 43.5 82 31.5 83 19.0 84 10.5 85 8.5 86 6.0 87 2.5 88 2.0 89 2.5 90 1.5 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05209569437888109 2 0.0 3 0.0 4 0.01616987760445871 5 0.0038468660429962264 6 2.1733706457605797E-5 7 1.7386965166084638E-4 8 3.6947300977929856E-4 9 0.0017386965166084638 10-11 7.824134324738086E-4 12-13 2.825381839488754E-4 14-15 4.3467412915211594E-5 16-17 8.693482583042319E-5 18-19 0.0 20-21 1.0866853228802899E-5 22-23 0.0 24-25 8.693482583042319E-5 26-27 1.0866853228802899E-5 28-29 0.0011084190293378956 30-31 9.67149937363458E-4 32-33 0.013366229471427566 34-35 0.0221140463206139 36-37 0.029938180645351982 38-39 0.020027610500683742 40-41 7.60679726016203E-5 42-43 0.001716962810150858 44-45 0.009584564547804156 46-47 0.014137776050672572 48-49 0.005607296266062296 50-51 0.010312643714133951 52-53 0.015909073126967446 54-55 0.009921436997897047 56-57 0.0029666509314631912 58-59 0.007867601737653298 60-61 0.0014887588923459972 62-63 9.888836438210638E-4 64-65 0.018767055526142606 66-67 0.04479316900912555 68-69 0.054812407686081824 70-71 0.048140159803596844 72-73 0.03978354967064741 74-75 0.02803648133031148 76-77 0.02837335378040437 78-79 0.012062207083971217 80-81 0.0020864358199301565 82-83 0.027025863980032813 84-85 0.04737948007758064 86-87 0.04827056204234247 88-89 0.05911568156468777 90-91 0.03415451969812751 92-93 0.02579790956517808 94-95 0.007248191103611534 96-97 0.003455659326759322 98-99 0.021396834007512907 100 0.018234579717931264 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4601148.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.54352720476518 #Duplication Level Percentage of deduplicated Percentage of total 1 80.09051385436116 54.09595801365666 2 13.034793737762138 17.608318908700795 3 3.422701023087614 6.935438990000878 4 1.2872346882962835 3.4777748475142984 5 0.6456730711617699 2.1805518323699644 6 0.3731911249573837 1.5123986940681584 7 0.25143999054246663 1.1888200689099697 8 0.16934366204168985 0.9150454595253953 9 0.12444589182652205 0.7564963032095362 >10 0.560869736017579 6.4110454812770685 >50 0.025540042729669952 1.1807820869660681 >100 0.012924191838033916 1.5056094025786257 >500 8.620446817772135E-4 0.41075687345132605 >1k 3.2326663563228444E-4 0.5319386171886153 >5k 3.591851507024535E-5 0.14447206354820225 >10k+ 1.0775554521073604E-4 1.1445923570345313 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19876 0.4319791495513728 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17186 0.3735154791804132 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10117 0.21987990823159786 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5955 0.12942422195504252 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4753 0.10330030679300037 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.693482583042319E-5 2 0.0 0.0 0.0 0.0 8.693482583042319E-5 3 0.0 0.0 0.0 0.0 8.693482583042319E-5 4 0.0 0.0 0.0 0.0 8.693482583042319E-5 5 0.0 0.0 0.0 0.0 1.0866853228802899E-4 6 0.0 0.0 0.0 0.0 1.521359452032406E-4 7 0.0 0.0 0.0 2.1733706457605797E-5 1.521359452032406E-4 8 0.0 0.0 0.0 2.1733706457605797E-5 1.521359452032406E-4 9 0.0 0.0 0.0 4.3467412915211594E-5 1.521359452032406E-4 10-11 2.1733706457605797E-5 0.0 0.0 5.433426614401449E-5 1.521359452032406E-4 12-13 2.1733706457605797E-5 0.0 0.0 7.606797260162028E-5 3.368724500928898E-4 14-15 2.1733706457605797E-5 0.0 0.0 9.78016790592261E-5 3.8033986300810144E-4 16-17 2.1733706457605797E-5 0.0 0.0 1.630027984320435E-4 4.5640783560972175E-4 18-19 2.1733706457605797E-5 0.0 0.0 3.4773930332169275E-4 4.7814154206732756E-4 20-21 2.1733706457605797E-5 0.0 0.0 0.0011084190293378956 4.998752485249334E-4 22-23 2.1733706457605797E-5 0.0 0.0 0.004292407025377145 4.998752485249334E-4 24-25 2.1733706457605797E-5 0.0 0.0 0.011214592532124592 4.998752485249334E-4 26-27 2.1733706457605797E-5 0.0 0.0 0.023222465349951794 5.433426614401449E-4 28-29 2.1733706457605797E-5 0.0 0.0 0.05785512659014663 5.433426614401449E-4 30-31 2.1733706457605797E-5 0.0 0.0 0.15997094638120748 5.759432211265537E-4 32-33 2.1733706457605797E-5 0.0 0.0 0.3771558750120622 6.302774872705681E-4 34-35 2.1733706457605797E-5 0.0 0.0 0.7051609728702488 6.628780469569768E-4 36-37 2.1733706457605797E-5 0.0 0.0 1.1941476344599216 6.737449001857798E-4 38-39 2.1733706457605797E-5 6.520111937281739E-5 0.0 1.8454850832879097 6.954786066433855E-4 40-41 2.1733706457605797E-5 6.520111937281739E-5 0.0 2.5089282066127847 6.954786066433855E-4 42-43 2.1733706457605797E-5 6.520111937281739E-5 0.0 3.1538976794486944 7.172123131009913E-4 44-45 2.1733706457605797E-5 6.520111937281739E-5 0.0 3.9080898940873015 7.498128727874E-4 46-47 2.1733706457605797E-5 6.520111937281739E-5 0.0 4.76476740152675 7.824134324738087E-4 48-49 2.1733706457605797E-5 6.520111937281739E-5 0.0 5.63458293451982 7.824134324738087E-4 50-51 2.1733706457605797E-5 6.520111937281739E-5 0.0 6.48315376945058 8.258808453890203E-4 52-53 2.1733706457605797E-5 6.520111937281739E-5 0.0 7.276477522566108 9.128156712194435E-4 54-55 2.1733706457605797E-5 6.520111937281739E-5 0.0 8.137393102764788 9.345493776770493E-4 56-57 2.1733706457605797E-5 6.520111937281739E-5 0.0 9.092600368429792 9.454162309058522E-4 58-59 2.1733706457605797E-5 6.520111937281739E-5 0.0 10.108412074551829 9.562830841346551E-4 60-61 2.1733706457605797E-5 6.520111937281739E-5 0.0 11.109249256924578 9.780167905922609E-4 62-63 2.1733706457605797E-5 6.520111937281739E-5 0.0 12.096991881156615 0.0010866853228802898 64-65 2.1733706457605797E-5 6.520111937281739E-5 0.0 13.061349037240273 0.0010866853228802898 66-67 2.1733706457605797E-5 6.520111937281739E-5 0.0 14.095101918043063 0.0010866853228802898 68-69 3.2600559686408696E-5 6.520111937281739E-5 0.0 15.184732158148357 0.0010866853228802898 70-71 4.3467412915211594E-5 6.520111937281739E-5 0.0 16.28027396641012 0.0010975521761090927 72-73 4.3467412915211594E-5 6.520111937281739E-5 0.0 17.31015824746346 0.0011518864422531074 74-75 4.3467412915211594E-5 6.520111937281739E-5 0.0 18.309213265906685 0.0012170875616259245 76-77 4.3467412915211594E-5 6.520111937281739E-5 0.0 19.366503750803062 0.0012388212680835304 78-79 4.3467412915211594E-5 6.520111937281739E-5 0.0 20.468185331139097 0.0012388212680835304 80-81 4.3467412915211594E-5 6.520111937281739E-5 0.0 21.568443353702165 0.0012388212680835304 82-83 4.3467412915211594E-5 6.520111937281739E-5 0.0 22.650684133611872 0.0012822886809987421 84-85 4.3467412915211594E-5 6.520111937281739E-5 0.0 23.670168836125246 0.0012822886809987421 86-87 4.3467412915211594E-5 6.520111937281739E-5 0.0 24.697401604990752 0.0013800903600579683 88 4.3467412915211594E-5 6.520111937281739E-5 0.0 25.474164273785586 0.0014778920391171943 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8675 0.0 56.027782 1 GTATCAA 15960 0.0 41.616123 1 TACCTGG 3295 0.0 38.220364 2 GTGGTAT 3220 0.0 33.57564 1 ATCAACG 19555 0.0 33.330627 3 CAACGCA 19765 0.0 32.924652 5 TATCAAC 20330 0.0 32.45228 2 TCAACGC 20585 0.0 31.663572 4 AACGCAG 21055 0.0 31.219164 6 ACCTGGG 3970 0.0 31.01242 3 ACGCAGA 23445 0.0 27.716011 7 TGGTATC 4000 0.0 27.60726 2 CGCAGAG 23805 0.0 27.237656 8 GTACATG 15270 0.0 24.718885 1 GCAGAGT 25765 0.0 24.636515 9 TACATGG 15570 0.0 23.691715 2 ACATGGG 15565 0.0 23.096024 3 GTACTAG 1715 0.0 22.475124 1 CATGGGG 11035 0.0 22.101887 4 GAGTACT 17340 0.0 22.068724 12-13 >>END_MODULE