FastQCFastQC Report
Mon 6 Jun 2016
ERR1211457_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211457_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7440605
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305280.4102892170730741No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262190.35237725964488104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139980.1881298630958101No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131650.055.890981
GTATCAA241650.041.541121
GTGGTAT39100.038.6731721
CAACGCA282600.034.564575
ATCAACG283950.034.433583
GTACTAG41400.033.6800651
TATCAAC298400.033.496432
AACGCAG311600.031.921186
TCAACGC317900.030.8741384
ACGCAGA340900.028.4881367
GCATCGT47700.028.1836178
CGCAGAG344400.027.9931378
GTACATG270050.026.7411161
TAGGCAT57900.026.6288915
TGGTATC56350.026.5568162
TACATGG273050.026.0242122
CTAGGCA63000.025.6668624
CTGTGCG49100.025.6562759
ACATGGG270400.025.4851993
GCAGAGT367100.025.442159
Produced by FastQC (version 0.11.5)