##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211457_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7440605 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42664070999603 31.0 31.0 34.0 30.0 34.0 2 31.484522427947727 31.0 31.0 34.0 28.0 34.0 3 31.544593752792952 31.0 31.0 34.0 28.0 34.0 4 35.18441672417767 37.0 35.0 37.0 32.0 37.0 5 35.238026880878635 37.0 35.0 37.0 32.0 37.0 6 35.303863193920385 37.0 35.0 37.0 32.0 37.0 7 35.2342281306426 37.0 35.0 37.0 32.0 37.0 8 35.2486469850234 37.0 35.0 37.0 32.0 37.0 9 36.8197172138556 39.0 37.0 39.0 32.0 39.0 10-11 36.75780383450002 39.0 37.0 39.0 32.0 39.0 12-13 36.6652260804061 39.0 37.0 39.0 32.0 39.0 14-15 37.84838921028599 40.0 37.0 41.0 32.0 41.0 16-17 37.85445760123001 40.0 37.0 41.0 32.0 41.0 18-19 37.8646427273051 40.0 37.0 41.0 32.0 41.0 20-21 37.8548088495492 40.0 37.0 41.0 32.0 41.0 22-23 37.729340557656265 40.0 37.0 41.0 32.0 41.0 24-25 37.63064455108153 40.0 37.0 41.0 32.0 41.0 26-27 37.48359837674491 40.0 37.0 41.0 31.5 41.0 28-29 37.3692825381807 40.0 36.5 41.0 31.0 41.0 30-31 37.20737399714136 39.5 36.0 41.0 31.0 41.0 32-33 37.017628405217046 39.0 36.0 41.0 30.5 41.0 34-35 36.890630130211186 39.0 35.0 41.0 30.0 41.0 36-37 36.748329067327184 39.0 35.0 41.0 30.0 41.0 38-39 36.585929437189584 39.0 35.0 41.0 30.0 41.0 40-41 36.40687309701295 39.0 35.0 40.0 30.0 41.0 42-43 36.19324940646628 39.0 35.0 40.0 29.0 41.0 44-45 36.012491188552545 38.5 35.0 40.0 28.0 41.0 46-47 35.746334673054136 38.0 34.5 40.0 27.0 41.0 48-49 35.4696110732931 38.0 34.0 40.0 26.5 41.0 50-51 34.76780167741737 37.5 33.5 39.5 25.5 40.5 52-53 35.04519410719962 38.0 34.0 39.5 26.0 40.5 54-55 35.429547395675485 38.0 34.0 40.0 27.0 41.0 56-57 35.317253502907356 38.0 34.0 40.0 26.0 41.0 58-59 35.076178012406245 38.0 34.0 40.0 26.0 41.0 60-61 34.84538488469687 37.0 34.0 40.0 26.0 41.0 62-63 34.573948422204914 37.0 33.5 40.0 26.0 41.0 64-65 34.360771200728976 36.0 33.0 40.0 25.5 41.0 66-67 34.16160822943833 36.0 33.0 39.5 25.0 41.0 68-69 33.82939713907673 35.5 33.0 39.0 25.0 41.0 70-71 33.49374755144239 35.0 33.0 39.0 24.0 41.0 72-73 33.14167725608334 35.0 32.0 38.0 23.5 40.0 74-75 32.7792780963376 35.0 32.0 37.0 23.0 40.0 76-77 32.4173655905669 35.0 32.0 37.0 21.5 39.0 78-79 32.03370035097952 35.0 31.0 36.0 20.0 39.0 80-81 31.65364866163437 35.0 31.0 36.0 20.0 38.5 82-83 31.297570426060783 35.0 31.0 35.0 19.0 37.0 84-85 30.983467540609936 34.0 30.0 35.0 18.5 37.0 86-87 30.662062224778765 34.0 30.0 35.0 18.0 36.5 88-89 30.357091728427996 34.0 29.5 35.0 16.5 36.0 90-91 30.050186510371134 34.0 29.0 35.0 15.0 36.0 92-93 29.754434148836015 34.0 29.0 35.0 11.5 35.5 94-95 29.486682668949634 34.0 29.0 35.0 10.0 35.0 96-97 29.203312902647028 34.0 27.0 35.0 10.0 35.0 98-99 28.89074349464862 34.0 27.0 35.0 10.0 35.0 100 27.63155066019497 32.0 24.0 35.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.46965090044190205 1101 2 -0.42212667900575696 1101 3 -0.5005537410248664 1101 4 -0.33571553673327514 1101 5 -0.3103243152677848 1101 6 -0.29736322087426004 1101 7 -0.3784196592372311 1101 8 -0.3877940008096843 1101 9 -0.6015599318077562 1101 10-11 -0.5428803307138281 1101 12-13 -0.5863930378611997 1101 14-15 -0.7492874727167163 1101 16-17 -0.6659502176178194 1101 18-19 -0.8065126744584532 1101 20-21 -0.8164974291934541 1101 22-23 -0.7187912653312978 1101 24-25 -0.9180671646172698 1101 26-27 -0.9350399709397621 1101 28-29 -0.967227196663579 1101 30-31 -0.9118988483158645 1101 32-33 -0.8715276088088402 1101 34-35 -0.8932814085626717 1101 36-37 -0.9247710457981739 1101 38-39 -0.9651586351691606 1101 40-41 -1.1209007549665344 1101 42-43 -1.1276238574370012 1101 44-45 -1.1128976041245195 1101 46-47 -1.0686861318677359 1101 48-49 -1.1722195261803776 1101 50-51 -0.963374612279388 1101 52-53 -0.8321622398762614 1101 54-55 -0.8021450081853772 1101 56-57 -0.7570731386356115 1101 58-59 -0.847303557941224 1101 60-61 -0.8718053498627185 1101 62-63 -0.9941442859089094 1101 64-65 -0.768123914092449 1101 66-67 -0.9101842402897589 1101 68-69 -0.9521129791784446 1101 70-71 -1.0038300210890867 1101 72-73 -1.0228712508570936 1101 74-75 -1.283549713238493 1101 76-77 -1.2532119208167316 1101 78-79 -1.24443645341114 1101 80-81 -1.506317180792724 1101 82-83 -1.4820066817564523 1101 84-85 -1.4960211965538797 1101 86-87 -1.6210776611942137 1101 88-89 -1.6844651302434954 1101 90-91 -1.7266198906338523 1101 92-93 -1.8925396054848882 1101 94-95 -1.9713662327886396 1101 96-97 -1.9828319038187274 1101 98-99 -2.171676529556148 1101 100 -2.027639094774049 1102 1 -0.3949527633800507 1102 2 -0.35540697177333413 1102 3 -0.4658045093246095 1102 4 -0.3651192140635331 1102 5 -0.2565434288582935 1102 6 -0.3278741870495381 1102 7 -0.3786295103923081 1102 8 -0.2582170733791713 1102 9 -0.4959884570597737 1102 10-11 -0.41419400979784626 1102 12-13 -0.4782827280405826 1102 14-15 -0.6684779554465123 1102 16-17 -0.6775572784484112 1102 18-19 -0.6361958937015828 1102 20-21 -0.7020670371791127 1102 22-23 -0.6409101516607265 1102 24-25 -0.643374045596417 1102 26-27 -0.7882504605862906 1102 28-29 -0.7968802821235741 1102 30-31 -0.7649780134032156 1102 32-33 -0.7398345492256055 1102 34-35 -0.7791445974843185 1102 36-37 -0.8147420375629153 1102 38-39 -0.8689685029999623 1102 40-41 -1.040697233182776 1102 42-43 -1.040047262882652 1102 44-45 -0.9700829759859388 1102 46-47 -1.0600652842567655 1102 48-49 -1.122864571091533 1102 50-51 -0.9492031154290359 1102 52-53 -0.8272122871741701 1102 54-55 -0.7533466981287944 1102 56-57 -0.743587057666268 1102 58-59 -0.7907377545372256 1102 60-61 -0.8748389344109242 1102 62-63 -0.8023461398643761 1102 64-65 -0.8252500654088593 1102 66-67 -0.9061123489996774 1102 68-69 -0.9303859150254752 1102 70-71 -0.9489594025938288 1102 72-73 -0.9122564852905839 1102 74-75 -1.0342161199374402 1102 76-77 -1.0532185030053824 1102 78-79 -1.0780763916036875 1102 80-81 -1.2154806216442893 1102 82-83 -1.323753661020259 1102 84-85 -1.3193569131374758 1102 86-87 -1.4094761291467108 1102 88-89 -1.4527160358690239 1102 90-91 -1.5389745565788395 1102 92-93 -1.6015074959598188 1102 94-95 -1.6384576772649417 1102 96-97 -1.7769449053778317 1102 98-99 -1.7973021646719118 1102 100 -1.8374006912210206 1103 1 -0.5007470781908943 1103 2 -0.5913312501825629 1103 3 -0.647063552196407 1103 4 -0.49855637137358144 1103 5 -0.3681644748820574 1103 6 -0.5007389073381532 1103 7 -0.5820287854154316 1103 8 -0.5178710945849758 1103 9 -0.5976612517275726 1103 10-11 -0.7004799081364439 1103 12-13 -0.8064308180244382 1103 14-15 -0.9153558727042181 1103 16-17 -0.9119071614953924 1103 18-19 -1.0076632195628932 1103 20-21 -0.9245903818759871 1103 22-23 -0.9097978737634165 1103 24-25 -0.9458914706262931 1103 26-27 -1.039821754132781 1103 28-29 -1.0532037192297352 1103 30-31 -0.9950553960999358 1103 32-33 -1.0811045605494485 1103 34-35 -0.9761194348860585 1103 36-37 -1.018688397361494 1103 38-39 -0.9897122211002198 1103 40-41 -1.14202378969064 1103 42-43 -1.2422591520485469 1103 44-45 -1.137156212477457 1103 46-47 -1.2397175757822225 1103 48-49 -1.3029008296453384 1103 50-51 -1.0990236966961433 1103 52-53 -0.8637532302897171 1103 54-55 -0.9191025314358612 1103 56-57 -0.9234628849151321 1103 58-59 -0.836022322450404 1103 60-61 -0.937269099052287 1103 62-63 -0.9296857855384104 1103 64-65 -0.8957145370170778 1103 66-67 -1.030023037161932 1103 68-69 -1.0126749398217285 1103 70-71 -1.064572836125194 1103 72-73 -1.059149092747674 1103 74-75 -1.2041578724769337 1103 76-77 -1.2839623283475419 1103 78-79 -1.2602717081186832 1103 80-81 -1.1283963731134747 1103 82-83 -1.1567429571342203 1103 84-85 -1.2966486980642387 1103 86-87 -1.4472028289919123 1103 88-89 -1.3455995060134676 1103 90-91 -1.3824117607128166 1103 92-93 -1.4958556488948958 1103 94-95 -1.4542911487504533 1103 96-97 -1.6436319825315664 1103 98-99 -1.7119553321501293 1103 100 -1.7591624543798332 1104 1 -0.4466560219142366 1104 2 -0.40061039269973264 1104 3 -0.36808569858711593 1104 4 -0.32953831891136787 1104 5 -0.3315297982961525 1104 6 -0.29179471036098903 1104 7 -0.31018208716858453 1104 8 -0.40781815282777956 1104 9 -0.418032578376625 1104 10-11 -0.47937637505911823 1104 12-13 -0.5066223178998115 1104 14-15 -0.6797635757694351 1104 16-17 -0.7372471931601581 1104 18-19 -0.715392428023705 1104 20-21 -0.7351807495161111 1104 22-23 -0.7229498688974232 1104 24-25 -0.7275830031780117 1104 26-27 -0.7118172535750276 1104 28-29 -0.895475951222032 1104 30-31 -0.9360051726493808 1104 32-33 -0.8657523460841858 1104 34-35 -0.9228324577813964 1104 36-37 -0.9394404033934123 1104 38-39 -1.0102430665240831 1104 40-41 -0.8813198000657323 1104 42-43 -0.9311893720680899 1104 44-45 -0.9532508382099394 1104 46-47 -1.0214915487456793 1104 48-49 -0.9886989223550771 1104 50-51 -1.0200488239339975 1104 52-53 -0.8507678497372169 1104 54-55 -0.7699491419953972 1104 56-57 -0.7703142260703899 1104 58-59 -0.7212984344975766 1104 60-61 -0.8835709627509161 1104 62-63 -0.9027350492083315 1104 64-65 -0.7921300335636374 1104 66-67 -0.863589750693663 1104 68-69 -0.959986587216882 1104 70-71 -0.8738398553719122 1104 72-73 -0.8949486715357224 1104 74-75 -0.9709623004862564 1104 76-77 -1.007833997316041 1104 78-79 -1.02578894028305 1104 80-81 -1.1129563887772633 1104 82-83 -1.1827131533556532 1104 84-85 -1.0598224455609362 1104 86-87 -1.1394391495975782 1104 88-89 -1.148176945902037 1104 90-91 -1.116069928894472 1104 92-93 -1.1716321421950262 1104 94-95 -1.1853452471284918 1104 96-97 -1.2675374662995935 1104 98-99 -1.5012444292193763 1104 100 -1.367288752091202 1105 1 -0.4600246114422468 1105 2 -0.4916140757720733 1105 3 -0.46162641960236783 1105 4 -0.3273352950688917 1105 5 -0.3717080087890636 1105 6 -0.3625248076047001 1105 7 -0.3610795345637001 1105 8 -0.34858822985229665 1105 9 -0.5187184240599052 1105 10-11 -0.49129655103945424 1105 12-13 -0.541485262564052 1105 14-15 -0.668173804533005 1105 16-17 -0.640617903291286 1105 18-19 -0.6552054523145827 1105 20-21 -0.6718789203451792 1105 22-23 -0.6273247020177593 1105 24-25 -0.6829451991343092 1105 26-27 -0.7334691677399476 1105 28-29 -0.6923673100997618 1105 30-31 -0.7107453371952701 1105 32-33 -0.8994844581131787 1105 34-35 -0.9194265203304397 1105 36-37 -0.9249053972209893 1105 38-39 -0.8980075771564842 1105 40-41 -0.8132453612060218 1105 42-43 -0.7647655089389644 1105 44-45 -0.9161290520659335 1105 46-47 -0.9605770966103293 1105 48-49 -0.9718707858157458 1105 50-51 -1.0079042733659804 1105 52-53 -0.7265127147693633 1105 54-55 -0.718214642355818 1105 56-57 -0.675578489568025 1105 58-59 -0.6202044555157542 1105 60-61 -0.6347307976275331 1105 62-63 -0.7432742505570928 1105 64-65 -0.7466005486026788 1105 66-67 -0.6453142324579986 1105 68-69 -0.6711334139725196 1105 70-71 -0.6327323756981116 1105 72-73 -0.7282781407344103 1105 74-75 -0.7046225523497043 1105 76-77 -0.8110035771155992 1105 78-79 -0.8216212502576461 1105 80-81 -0.8034192034027505 1105 82-83 -0.8723862407703606 1105 84-85 -0.7875179172707156 1105 86-87 -0.9285302855954143 1105 88-89 -1.0102534749613454 1105 90-91 -1.0257245712233747 1105 92-93 -1.0497623035748802 1105 94-95 -1.1336800471344688 1105 96-97 -1.2268599544082512 1105 98-99 -1.164902102728366 1105 100 -1.3129084454878317 1106 1 -0.5440617233057417 1106 2 -0.5584177095074025 1106 3 -0.547386870929067 1106 4 -0.43948230104550134 1106 5 -0.4868031591119717 1106 6 -0.48786551882398754 1106 7 -0.5280105500107766 1106 8 -0.4846411410224647 1106 9 -0.6711602081503258 1106 10-11 -0.7147950013302165 1106 12-13 -0.7360922844308107 1106 14-15 -0.8455607285280919 1106 16-17 -0.8014244664052086 1106 18-19 -0.8441503504144379 1106 20-21 -0.8796639919283038 1106 22-23 -0.7705348814664106 1106 24-25 -0.9264483227040472 1106 26-27 -0.836421839664446 1106 28-29 -0.8912979917292674 1106 30-31 -0.9526874691097333 1106 32-33 -0.9631410492588444 1106 34-35 -0.93822984070475 1106 36-37 -0.8988801303704506 1106 38-39 -0.912415295406106 1106 40-41 -0.8617388758311861 1106 42-43 -0.8553159459723787 1106 44-45 -0.9656978404237933 1106 46-47 -1.0044840534377713 1106 48-49 -0.900467287085327 1106 50-51 -0.9506230300182708 1106 52-53 -0.9293555245597744 1106 54-55 -0.9291367161177249 1106 56-57 -1.0125267152395594 1106 58-59 -0.8875530330734236 1106 60-61 -0.7999467710403607 1106 62-63 -0.8840661730402744 1106 64-65 -0.9171608072870825 1106 66-67 -0.899339623133308 1106 68-69 -0.8969271731620552 1106 70-71 -0.9596579334199689 1106 72-73 -0.8753517074720207 1106 74-75 -0.8943613317521937 1106 76-77 -0.998456868852827 1106 78-79 -0.9210981970359136 1106 80-81 -0.97098405892541 1106 82-83 -0.9921866282175031 1106 84-85 -0.9402629175948896 1106 86-87 -0.9364599140463525 1106 88-89 -1.0009619931499145 1106 90-91 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1.3023116905293861 2311 46-47 1.347639548000231 2311 48-49 1.3814133882327653 2311 50-51 1.3213451533739686 2311 52-53 1.1855326512626476 2311 54-55 1.1019648565930353 2311 56-57 1.1488814338913258 2311 58-59 1.0356244665963814 2311 60-61 1.1024337905988801 2311 62-63 1.101203774805441 2311 64-65 1.0761774477491173 2311 66-67 1.1195444793064482 2311 68-69 1.0749745278152503 2311 70-71 1.0431488253462788 2311 72-73 1.0544646453293112 2311 74-75 1.0467021552578686 2311 76-77 1.0813185135648027 2311 78-79 1.1883641052828047 2311 80-81 1.2296481154093186 2311 82-83 1.2219729598050861 2311 84-85 1.2306264091437562 2311 86-87 1.3488157588108791 2311 88-89 1.283556409353384 2311 90-91 1.407967571239201 2311 92-93 1.5039847506434008 2311 94-95 1.5319415873223363 2311 96-97 1.5413565511097538 2311 98-99 1.6183210991028893 2311 100 1.6913815664741314 2312 1 0.5402002450024632 2312 2 0.5632842863241478 2312 3 0.6704479701389694 2312 4 0.5344667645470338 2312 5 0.5477826528244449 2312 6 0.4466547972778727 2312 7 0.5824684077672657 2312 8 0.6301494802878125 2312 9 0.7921331851720197 2312 10-11 0.8230159802819657 2312 12-13 0.8576352756402201 2312 14-15 1.0510777078990188 2312 16-17 1.041976825791906 2312 18-19 1.0050114258460425 2312 20-21 1.0367562482127681 2312 22-23 1.0884221164030876 2312 24-25 1.071762267548202 2312 26-27 1.098916416277497 2312 28-29 1.1771579770141471 2312 30-31 1.1152347618133334 2312 32-33 1.1980425894459543 2312 34-35 1.1819416254849813 2312 36-37 1.201635490704156 2312 38-39 1.2313595316879855 2312 40-41 1.2317419912834353 2312 42-43 1.295224067529567 2312 44-45 1.259118622257951 2312 46-47 1.2299714474641306 2312 48-49 1.2743066878476768 2312 50-51 1.2181067367590757 2312 52-53 1.1331591057877546 2312 54-55 1.1223925544325084 2312 56-57 1.0772865861994276 2312 58-59 1.0251968315083175 2312 60-61 0.9871100822841115 2312 62-63 1.0583096385052215 2312 64-65 1.0396354262755878 2312 66-67 1.0172761470085092 2312 68-69 1.0321376260191002 2312 70-71 1.0376193551981672 2312 72-73 1.0791540259102632 2312 74-75 1.0993848559525219 2312 76-77 1.044391303094521 2312 78-79 1.0351029674326249 2312 80-81 0.917836498243048 2312 82-83 1.127957456026408 2312 84-85 1.1827874104050657 2312 86-87 1.3013622889723386 2312 88-89 1.3408537171092476 2312 90-91 1.3702331424756302 2312 92-93 1.379601858347808 2312 94-95 1.3943925889392297 2312 96-97 1.4830322905826812 2312 98-99 1.352476031258167 2312 100 1.437917689316265 2313 1 0.5575859349167587 2313 2 0.625421115573598 2313 3 0.6453084711803179 2313 4 0.5752148515310367 2313 5 0.5950903106500363 2313 6 0.6023125629330579 2313 7 0.6298097696057425 2313 8 0.6523206749704116 2313 9 0.79099045248838 2313 10-11 0.676832801491031 2313 12-13 0.8023152024912719 2313 14-15 1.0519076611422875 2313 16-17 1.0237502149429147 2313 18-19 1.0134181871629835 2313 20-21 1.090721972949808 2313 22-23 1.1117611506106542 2313 24-25 1.1641964336251505 2313 26-27 1.164584241463082 2313 28-29 1.1699945759808301 2313 30-31 1.1522945459371243 2313 32-33 1.2227435809820975 2313 34-35 1.2252306449526955 2313 36-37 1.2382162166593957 2313 38-39 1.326635789620184 2313 40-41 1.3399507528282513 2313 42-43 1.2831129522845828 2313 44-45 1.2276480718658647 2313 46-47 1.230131589969183 2313 48-49 1.2483292262597843 2313 50-51 1.2375653397309705 2313 52-53 1.1108255724781415 2313 54-55 1.046758430676121 2313 56-57 1.066055521615091 2313 58-59 1.055063599248264 2313 60-61 1.0737281875269318 2313 62-63 1.0965019691822846 2313 64-65 1.0557396015168266 2313 66-67 1.0437860519060962 2313 68-69 1.1032818430228204 2313 70-71 1.0989652382058424 2313 72-73 1.1756063523655982 2313 74-75 1.1650360487438647 2313 76-77 1.1251066136042027 2313 78-79 1.1726321209516293 2313 80-81 1.2670447768994038 2313 82-83 1.248852561749004 2313 84-85 1.3448664558102728 2313 86-87 1.4181179437926055 2313 88-89 1.4705896476539202 2313 90-91 1.5754790060799202 2313 92-93 1.5503516776844783 2313 94-95 1.6154911589021204 2313 96-97 1.6751498856792821 2313 98-99 1.787438047391138 2313 100 1.8887221933815468 2314 1 0.5370217147244851 2314 2 0.5458842855978538 2314 3 0.5733205742054999 2314 4 0.4996527040808303 2314 5 0.4952425885265015 2314 6 0.5276745582331444 2314 7 0.54536127548959 2314 8 0.5365312349045936 2314 9 0.6671714843115168 2314 10-11 0.7051594247610566 2314 12-13 0.49266970206618765 2314 14-15 0.8757454404913929 2314 16-17 0.8699758442548671 2314 18-19 0.9043609111356972 2314 20-21 0.8978916484459134 2314 22-23 0.8051897598166917 2314 24-25 0.8137215233854178 2314 26-27 0.9071064012794849 2314 28-29 0.9539860767802324 2314 30-31 0.868098741115972 2314 32-33 0.9773096816528479 2314 34-35 1.0852980074730425 2314 36-37 1.1109162554314906 2314 38-39 1.1393540263650195 2314 40-41 1.1348014738806071 2314 42-43 1.227842470622761 2314 44-45 1.2852567279433416 2314 46-47 1.2346378078760196 2314 48-49 1.271972761891469 2314 50-51 1.1931112501693448 2314 52-53 1.0632579624423641 2314 54-55 1.0164114427951176 2314 56-57 1.0534868161239572 2314 58-59 1.0368572269556822 2314 60-61 1.0514164126273542 2314 62-63 0.9949613054321489 2314 64-65 0.9438685382097205 2314 66-67 1.002021001850487 2314 68-69 1.00160301775977 2314 70-71 1.0882460318392972 2314 72-73 1.025108000019891 2314 74-75 1.1060098159413982 2314 76-77 1.1126459206504435 2314 78-79 1.1954072127878312 2314 80-81 1.3211370851420128 2314 82-83 1.1799976990450176 2314 84-85 1.2326451342248212 2314 86-87 1.349031981526096 2314 88-89 1.4979051943691815 2314 90-91 1.5855431748528659 2314 92-93 1.6719947776890152 2314 94-95 1.7569070046160142 2314 96-97 1.8350959177355648 2314 98-99 1.8959142849522905 2314 100 1.8691410725167295 2315 1 0.43933181540918653 2315 2 0.4874770527864847 2315 3 0.4848931124124647 2315 4 0.4180525236006929 2315 5 0.36284390351035967 2315 6 -0.4205917970285924 2315 7 0.2414986854996286 2315 8 0.263029800267617 2315 9 0.5120669428951317 2315 10-11 0.6056765043443875 2315 12-13 0.6080270457267716 2315 14-15 0.759619580609602 2315 16-17 0.6703920290409187 2315 18-19 0.7200542539799173 2315 20-21 0.7978037094009736 2315 22-23 0.6942758140247633 2315 24-25 0.7614640710765883 2315 26-27 0.8733380331240213 2315 28-29 0.9575775964149429 2315 30-31 0.9848483404380701 2315 32-33 0.9861273967183379 2315 34-35 0.9695819545455464 2315 36-37 0.9872940296632535 2315 38-39 1.0231962773244447 2315 40-41 1.0609136927057676 2315 42-43 1.0730054479847126 2315 44-45 1.0997700218458348 2315 46-47 1.1299994859610862 2315 48-49 1.097701285355889 2315 50-51 1.0682037662464907 2315 52-53 0.8989445823015743 2315 54-55 0.7494562646960432 2315 56-57 0.7260410051839017 2315 58-59 0.6858879716063129 2315 60-61 0.6277049597422675 2315 62-63 0.8372480432437328 2315 64-65 0.8788147668352337 2315 66-67 0.8756102879935383 2315 68-69 0.8818876266261029 2315 70-71 0.9021447028492275 2315 72-73 0.979802421843182 2315 74-75 1.0097120722431967 2315 76-77 0.9788321073173165 2315 78-79 0.9907199691516588 2315 80-81 1.0491787981626004 2315 82-83 1.183991985007978 2315 84-85 1.158401682632313 2315 86-87 1.305969874450554 2315 88-89 1.4488661210206146 2315 90-91 1.5113525467139688 2315 92-93 1.575606481285412 2315 94-95 1.7192485217496234 2315 96-97 1.7852007392763305 2315 98-99 1.877455939446886 2315 100 1.8529515843840016 2316 1 0.05214584086703411 2316 2 -0.036020578689687 2316 3 0.08802056547096981 2316 4 0.05820339907325689 2316 5 0.0563996824212154 2316 6 0.164630731045456 2316 7 0.18806449579410867 2316 8 0.19711371612348216 2316 9 0.2943435393873344 2316 10-11 0.17337388262416198 2316 12-13 0.243245720727721 2316 14-15 0.43112083494504105 2316 16-17 0.5127766872490724 2316 18-19 0.4732755250010072 2316 20-21 0.4020992162324646 2316 22-23 0.32090361609211016 2316 24-25 0.3873243157477049 2316 26-27 0.447988048885712 2316 28-29 0.5263939430339093 2316 30-31 0.5179517098485888 2316 32-33 0.6078095804965287 2316 34-35 0.6274308828283068 2316 36-37 0.6707566374813538 2316 38-39 0.7289489979628598 2316 40-41 0.7418324351791696 2316 42-43 0.7367199764190318 2316 44-45 0.5918844699576553 2316 46-47 0.5750990781953149 2316 48-49 0.6652107171353308 2316 50-51 0.4594836552236288 2316 52-53 0.2830521261334411 2316 54-55 0.07854718984509645 2316 56-57 0.2595472366804117 2316 58-59 0.2571201562385852 2316 60-61 0.35347070706763617 2316 62-63 0.36467273992825966 2316 64-65 0.3386356460075177 2316 66-67 0.3562955094879854 2316 68-69 0.4296443203943383 2316 70-71 0.34579384841813265 2316 72-73 0.4260078557632667 2316 74-75 0.559918767292551 2316 76-77 0.6437669292374082 2316 78-79 0.6835868171359927 2316 80-81 0.8237041786560013 2316 82-83 0.8808651797898506 2316 84-85 0.9102090238332892 2316 86-87 1.116105463316586 2316 88-89 1.2443206400056894 2316 90-91 1.2728127580564852 2316 92-93 1.4534350445724762 2316 94-95 1.4792593904244704 2316 96-97 1.5742783009584969 2316 98-99 1.6722018132617507 2316 100 1.524025603762336 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 32.0 1 350.0 2 187.0 3 148.0 4 136.0 5 109.0 6 100.0 7 485.0 8 1659.0 9 3338.0 10 4949.0 11 6621.0 12 8569.0 13 10821.0 14 12942.0 15 16613.0 16 22006.0 17 29055.0 18 38226.0 19 48717.0 20 61429.0 21 75130.0 22 80914.0 23 81460.0 24 80291.0 25 83346.0 26 93227.0 27 107216.0 28 128156.0 29 151997.0 30 183654.0 31 228022.0 32 288435.0 33 371444.0 34 507956.0 35 700516.0 36 1017754.0 37 1393741.0 38 1234216.0 39 366233.0 40 405.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.946374364987584 20.134260729585122 12.588121904066154 23.33124300136114 2 16.022595737120646 20.74099696893019 39.79871381424872 23.437693479700446 3 18.903498471610703 26.716156215514786 30.123538808344325 24.256806504530186 4 12.55348022204702 17.109100526753384 39.29211926659153 31.04529998460807 5 12.771754108088357 38.15031232952143 35.36652910527198 13.711404457118245 6 29.411853297750827 38.285609552949765 18.141307320777976 14.16122982852142 7 25.754323415904018 33.2077662936144 23.41820359880551 17.61970669167608 8 25.50160832280325 34.873327686509576 20.73314400920094 18.891919981486236 9 26.186688150388925 15.261088670236203 21.442933840344878 37.10928933903 10-11 24.718010601787583 26.14355102740532 28.431110229682986 20.707328141124112 12-13 25.322376482488124 24.443826408428006 28.434461213209183 21.79933589587469 14-15 22.674585657379353 25.66767755541332 26.27778647424539 25.379950312961935 16-17 21.428093270992083 28.436799368325033 27.414770187769793 22.72033717291309 18-19 20.987282422697803 27.855755439684593 29.865632699806838 21.291329437810766 20-21 22.05350551806615 27.115190357604778 29.549300184183103 21.28200394014597 22-23 22.398097221516462 26.859619675523195 29.219279276808667 21.523003826151673 24-25 21.937137876844503 27.00995217586602 29.650815197391754 21.402094749897717 26-27 21.778554738604097 27.149108259342263 29.246660530289205 21.825676471764428 28-29 21.33062850222834 27.63856294455318 29.307353136186602 21.72345541703187 30-31 21.803278611398675 27.31141807883822 29.33147359541587 21.553829714347238 32-33 21.96223218683524 27.575903698031716 28.706682343097672 21.75518177203537 34-35 22.05947433556606 27.25194784384125 29.348829062133923 21.33974875845876 36-37 21.890254427312705 26.855615887672567 29.317638110540052 21.936491574474676 38-39 21.832684195396176 26.848023351624622 29.643047957522604 21.676244495456594 40-41 21.60866323438682 26.394985699804856 30.02436050727767 21.971990558530653 42-43 21.435510774615484 26.657914822780203 29.46437303827117 22.442201364333144 44-45 21.29419216364532 27.29308779651464 29.45167244261163 21.961047597228404 46-47 21.373418476345602 27.050715111755245 29.145871342406167 22.42999506949299 48-49 21.711876971090327 26.85691137132863 28.988346008791872 22.442865648789173 50-51 21.415253308210247 26.955352277414907 28.815392299857372 22.814002114517475 52-53 20.95377710215892 27.375410775263227 28.7728836494067 22.897928473171152 54-55 20.907822167963236 27.30941381470855 28.764033462497213 23.018730554831006 56-57 21.54058593748687 26.921702114214014 28.49385151190319 23.04386043639593 58-59 21.09146013306849 26.962165265647887 28.793747871747872 23.152626729535754 60-61 21.044448755128627 27.084745676130588 28.48621318179441 23.38459238694637 62-63 21.24741431315251 26.7027321320847 28.37030900533185 23.67954454943094 64-65 20.866211858826745 26.75199556484386 28.440788272647026 23.941004303682366 66-67 21.378504916332243 26.54269556450217 28.172297519621196 23.906501999544385 68-69 21.23619223702633 26.53540737824794 28.270202335942358 23.958198048783373 70-71 21.34301490513357 26.32533958753514 28.180652999224364 24.15099250810693 72-73 21.8165834013784 26.24478771217052 28.240351259090286 23.6982776273608 74-75 21.911394163712625 26.398260799778566 28.058711988182537 23.631633048326268 76-77 21.933448165172884 26.438982568752582 27.838747653672048 23.788821612402483 78-79 22.070741215619453 26.499246941238564 27.707924904223518 23.722086938918462 80-81 22.07770307055383 26.498027856543132 27.908303327519917 23.51596574538312 82-83 22.442612222369863 25.818906281578762 28.471396224599122 23.267085271452252 84-85 22.6760860698334 26.02532518063948 27.807132484769813 23.4914562647573 86-87 22.334809942443094 26.21788664177178 28.006404307281745 23.440899108503384 88-89 23.08243694354539 25.582811307429232 27.68277762140076 23.651974127624616 90-91 23.34250212136742 25.53637746057617 27.576486007376477 23.54463441067993 92-93 23.67908918095904 25.37999913834757 27.57414798067183 23.366763700021558 94-95 23.139757011133568 25.182626115243778 27.87064432438638 23.806972549236274 96-97 23.49275838569305 24.99738030585526 27.580072741126294 23.9297885673254 98-99 23.601508888707997 25.216878049062473 27.333547580347506 23.84806548188202 100 24.42159272443442 24.85875151761373 26.85902388483034 23.86063187312151 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3207.0 1 2175.0 2 1573.5 3 2144.0 4 1889.0 5 1729.5 6 2525.0 7 4178.5 8 6458.0 9 7733.0 10 6711.5 11 5428.5 12 5632.0 13 6360.5 14 7121.5 15 8158.0 16 9096.5 17 10080.5 18 11144.5 19 12151.5 20 13309.0 21 14663.5 22 16833.0 23 19878.0 24 23476.5 25 27937.5 26 33780.0 27 40535.5 28 49050.0 29 61968.0 30 78574.5 31 94695.5 32 115279.0 33 133921.0 34 148646.5 35 164950.0 36 180849.0 37 205469.0 38 220909.0 39 217117.0 40 210177.0 41 206490.0 42 211071.5 43 224021.0 44 242702.0 45 268846.0 46 292112.5 47 313812.5 48 329407.0 49 327843.5 50 316695.0 51 313095.0 52 309908.5 53 295999.5 54 278480.5 55 248100.5 56 218662.5 57 184371.5 58 149062.0 59 130114.5 60 106050.5 61 79135.5 62 60199.5 63 46084.5 64 32786.0 65 20417.5 66 13422.0 67 9656.5 68 6910.0 69 4930.5 70 3635.0 71 2815.5 72 2156.0 73 1530.0 74 1120.5 75 837.5 76 585.5 77 379.5 78 270.5 79 220.5 80 164.5 81 114.0 82 87.0 83 73.0 84 63.0 85 46.5 86 37.5 87 29.0 88 23.0 89 23.5 90 21.5 91 21.0 92 17.5 93 12.5 94 9.5 95 5.5 96 2.5 97 1.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21432396962343786 2 0.14629186739519165 3 0.023223917947532494 4 0.02204121842242667 5 0.026704817686196217 6 0.05363811141701515 7 0.060089199735774174 8 0.05343651490705393 9 0.04080313361615084 10-11 0.042738460111778544 12-13 0.04121304651973865 14-15 0.0193734246072732 16-17 0.01795552915387929 18-19 0.017404498693318622 20-21 0.020703961573017248 22-23 0.0241781414280156 24-25 0.02121467273158567 26-27 0.018311682988144107 28-29 0.017787532062244937 30-31 0.01620163951721668 32-33 0.024688852586584018 34-35 0.025092045606506456 36-37 0.023936225616062134 38-39 0.02199417923676905 40-41 0.02312311969255188 42-43 0.029197894526049963 44-45 0.038451174333269944 46-47 0.04349108708230043 48-49 0.03949275630140291 50-51 0.02726256803042226 52-53 0.04225462848787162 54-55 0.040655296175512605 56-57 0.04310805371337412 58-59 0.04646799554606111 60-61 0.0231701588782095 62-63 0.024426777123634436 64-65 0.02498452746786048 66-67 0.030716588234424483 68-69 0.03466115994599901 70-71 0.03806142108067825 72-73 0.042093351279902644 74-75 0.023969825034389002 76-77 0.017753932643918068 78-79 0.01718274253236128 80-81 0.020038693090145224 82-83 0.018392321592128596 84-85 0.011497720951454888 86-87 0.012425064897276497 88-89 0.017727053109256572 90-91 0.019050870191335247 92-93 0.01895679182002001 94-95 0.022908083415259916 96-97 0.023647270618451052 98-99 0.02890221964477351 100 0.0284654272065242 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7440605.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.924131595981535 #Duplication Level Percentage of deduplicated Percentage of total 1 72.53413674635722 42.740110188432716 2 15.608630553263184 18.394500015070754 3 5.523495043697901 9.764014464738207 4 2.4332630229396957 5.735116422853379 5 1.2397740711174443 3.6526305257905025 6 0.715657994020444 2.5301715490426084 7 0.47155828722289395 1.9450313800048222 8 0.31234833610678614 1.4723883568433709 9 0.21912476913280085 1.1620563059088207 >10 0.9067293904554234 8.753990556489729 >50 0.026495151226532867 1.0460345494016838 >100 0.007748518303626505 0.7422332485909499 >500 4.4892323750692054E-4 0.1735269576734913 >1k 4.7696569610241593E-4 0.6276956736254081 >5k 2.8056805630253288E-5 0.09092725989159561 >10k+ 8.417041689075987E-5 1.1695725456419876 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30528 0.4102892170730741 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26219 0.35237725964488104 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13998 0.1881298630958101 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.031930199224391E-5 2 0.0 0.0 0.0 1.343976733074797E-5 4.031930199224391E-5 3 0.0 0.0 0.0 1.343976733074797E-5 4.031930199224391E-5 4 0.0 0.0 0.0 1.343976733074797E-5 4.031930199224391E-5 5 0.0 2.687953466149594E-5 0.0 1.343976733074797E-5 4.031930199224391E-5 6 0.0 2.687953466149594E-5 0.0 1.343976733074797E-5 5.375906932299188E-5 7 0.0 2.687953466149594E-5 0.0 1.343976733074797E-5 5.375906932299188E-5 8 0.0 2.687953466149594E-5 0.0 1.343976733074797E-5 5.375906932299188E-5 9 0.0 2.687953466149594E-5 0.0 2.687953466149594E-5 5.375906932299188E-5 10-11 0.0 2.687953466149594E-5 0.0 6.719883665373985E-5 5.375906932299188E-5 12-13 0.0 4.031930199224391E-5 0.0 1.2767778964210572E-4 1.6799709163434963E-4 14-15 0.0 4.031930199224391E-5 0.0 1.5455732430360165E-4 2.0831639362659353E-4 16-17 0.0 4.031930199224391E-5 0.0 2.1503627729196754E-4 2.687953466149594E-4 18-19 0.0 4.031930199224391E-5 0.0 2.687953466149594E-4 2.8223511394570735E-4 20-21 0.0 5.375906932299188E-5 0.0 5.644702278914148E-4 3.2255441593795125E-4 22-23 0.0 5.375906932299188E-5 0.0 0.002150362772919675 3.3599418326869926E-4 24-25 0.0 6.719883665373985E-5 0.0 0.005308708095645448 3.3599418326869926E-4 26-27 0.0 8.063860398448783E-5 0.0 0.012169709317992286 3.628737179301952E-4 28-29 0.0 9.407837131523579E-5 0.0 0.038706529912554154 3.7631348526094316E-4 30-31 0.0 9.407837131523579E-5 0.0 0.12681764453293787 3.7631348526094316E-4 32-33 0.0 9.407837131523579E-5 0.0 0.3154447790199856 3.7631348526094316E-4 34-35 0.0 1.0751813864598376E-4 0.0 0.6117177299426593 3.7631348526094316E-4 36-37 0.0 1.2095790597673172E-4 0.0 1.072943665199268 3.7631348526094316E-4 38-39 0.0 1.343976733074797E-4 0.0 1.6568813960692712 3.7631348526094316E-4 40-41 0.0 1.343976733074797E-4 0.0 2.209040797085721 3.7631348526094316E-4 42-43 0.0 1.343976733074797E-4 0.0 2.7530624189834025 3.897532525916911E-4 44-45 0.0 1.343976733074797E-4 0.0 3.420555183348666 3.9647313625706514E-4 46-47 0.0 1.343976733074797E-4 0.0 4.205699670927297 4.300725545839351E-4 48-49 0.0 1.343976733074797E-4 0.0 5.003692576074123 4.905515075723009E-4 50-51 0.0 1.343976733074797E-4 0.0 5.775860968294917 5.107111585684229E-4 52-53 0.0 1.343976733074797E-4 0.0 6.484257664531311 5.107111585684229E-4 54-55 0.0 1.4783744063822768E-4 0.0 7.247287014967197 5.107111585684229E-4 56-57 0.0 1.4783744063822768E-4 0.0 8.13335743531608 5.241509258991708E-4 58-59 0.0 1.6127720796897565E-4 0.0 9.098830001055022 5.241509258991708E-4 60-61 0.0 1.6127720796897565E-4 0.0 10.031294498229645 5.241509258991708E-4 62-63 0.0 1.6127720796897565E-4 0.0 10.919219606470172 5.644702278914147E-4 64-65 0.0 1.747169752997236E-4 0.0 11.812648299432642 5.644702278914147E-4 66-67 0.0 1.8815674263047158E-4 0.0 12.784874348255283 5.779099952221626E-4 68-69 0.0 2.0159650996121956E-4 0.0 13.820334502369095 5.913497625529107E-4 70-71 0.0 2.1503627729196754E-4 0.0 14.869234423813655 5.913497625529107E-4 72-73 0.0 2.284760446227155E-4 0.0 15.87876254686279 6.518287155412765E-4 74-75 0.0 2.284760446227155E-4 0.0 16.831111179803255 7.190275521950164E-4 76-77 0.0 2.3519592828808946E-4 0.0 17.846492321524927 7.391872031911384E-4 78-79 0.0 2.4191581195346347E-4 0.0 18.902333882795823 7.660667378526343E-4 80-81 0.0 2.8223511394570735E-4 0.0 19.97909444191702 7.862263888487563E-4 82-83 1.343976733074797E-5 2.889549976110813E-4 0.0 21.03121990752096 8.467053418371221E-4 84-85 1.343976733074797E-5 2.9567488127645535E-4 0.0 22.018653053078346 8.467053418371221E-4 86-87 1.343976733074797E-5 2.9567488127645535E-4 0.0 23.00405007388512 8.73584876498618E-4 88 2.687953466149594E-5 2.9567488127645535E-4 0.0 23.761911833782335 9.00464411160114E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13165 0.0 55.89098 1 GTATCAA 24165 0.0 41.54112 1 GTGGTAT 3910 0.0 38.673172 1 CAACGCA 28260 0.0 34.56457 5 ATCAACG 28395 0.0 34.43358 3 GTACTAG 4140 0.0 33.680065 1 TATCAAC 29840 0.0 33.49643 2 AACGCAG 31160 0.0 31.92118 6 TCAACGC 31790 0.0 30.874138 4 ACGCAGA 34090 0.0 28.488136 7 GCATCGT 4770 0.0 28.183617 8 CGCAGAG 34440 0.0 27.993137 8 GTACATG 27005 0.0 26.741116 1 TAGGCAT 5790 0.0 26.628891 5 TGGTATC 5635 0.0 26.556816 2 TACATGG 27305 0.0 26.024212 2 CTAGGCA 6300 0.0 25.666862 4 CTGTGCG 4910 0.0 25.656275 9 ACATGGG 27040 0.0 25.485199 3 GCAGAGT 36710 0.0 25.44215 9 >>END_MODULE