FastQCFastQC Report
Mon 6 Jun 2016
ERR1211457_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211457_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7440605
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280350.3767838771175194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254310.34178672298825163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137060.18420545103523167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140200.058.0169071
GTATCAA255400.040.6843951
GTGGTAT40700.037.6599431
GTACTAG41100.036.8358351
CAACGCA305000.033.4623685
ATCAACG305150.033.359133
TATCAAC320150.032.3975872
AACGCAG332400.031.3110546
TCAACGC337900.030.3762444
ACGCAGA366150.027.8474667
CGCAGAG371700.027.38118
CTGTGCG52550.027.0032639
TGGTATC57250.026.843312
TAGTACT55650.025.9264374
GCAGAGT396750.025.1784139
GCATCGT55450.024.0658828
CTAACGC22300.024.0253473
GTACATG274500.023.9624841
CTAGTAC59700.023.9314423
TACATGG279400.023.4645182
Produced by FastQC (version 0.11.5)