##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211457_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7440605 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.018059418555346 33.0 31.0 34.0 30.0 34.0 2 32.16419619103554 33.0 31.0 34.0 30.0 34.0 3 32.23556001158508 34.0 31.0 34.0 30.0 34.0 4 35.891297011466136 37.0 35.0 37.0 35.0 37.0 5 35.74569151298853 37.0 35.0 37.0 35.0 37.0 6 35.783837739001065 37.0 35.0 37.0 33.0 37.0 7 35.71312601596241 37.0 35.0 37.0 33.0 37.0 8 35.70313852166591 37.0 35.0 37.0 33.0 37.0 9 37.27169201966775 39.0 37.0 39.0 34.0 39.0 10-11 37.25632599768433 39.0 37.0 39.0 33.5 39.0 12-13 37.16948453788368 39.0 37.0 39.0 33.0 39.0 14-15 38.35647443991449 40.0 38.0 41.0 33.0 41.0 16-17 38.35504955578209 40.0 38.0 41.0 33.0 41.0 18-19 38.36627639822299 40.0 38.0 41.0 33.5 41.0 20-21 38.32884348786153 40.0 38.0 41.0 34.0 41.0 22-23 38.22473763894199 40.0 38.0 41.0 33.5 41.0 24-25 38.097773635342826 40.0 37.0 41.0 33.0 41.0 26-27 37.95751494670124 40.0 37.0 41.0 33.0 41.0 28-29 37.89253998834772 40.0 37.0 41.0 33.0 41.0 30-31 37.930620493898004 40.0 37.0 41.0 33.0 41.0 32-33 37.76386671513943 40.0 37.0 41.0 33.0 41.0 34-35 37.646918698143494 40.0 36.5 41.0 33.0 41.0 36-37 37.49462597463513 40.0 36.0 41.0 32.0 41.0 38-39 37.36683569414046 40.0 36.0 41.0 32.0 41.0 40-41 37.28365395018282 39.0 36.0 41.0 32.0 41.0 42-43 37.12935755896194 39.0 36.0 41.0 31.0 41.0 44-45 36.93483896806778 39.0 35.5 41.0 31.0 41.0 46-47 36.67546711591329 39.0 35.0 41.0 30.0 41.0 48-49 36.414717015619026 39.0 35.0 41.0 30.0 41.0 50-51 36.20636567859737 39.0 35.0 40.0 29.0 41.0 52-53 35.98195355082012 38.5 35.0 40.0 28.5 41.0 54-55 35.76962693221854 38.0 35.0 40.0 28.0 41.0 56-57 35.513253223360195 38.0 34.0 40.0 28.0 41.0 58-59 35.221300284049484 37.5 34.0 40.0 27.0 41.0 60-61 34.96709837439295 37.0 34.0 40.0 27.0 41.0 62-63 34.67523259197337 36.5 34.0 40.0 26.0 41.0 64-65 34.32173808447028 36.0 33.0 39.0 26.0 41.0 66-67 33.96965757488806 35.5 33.0 39.0 26.0 40.0 68-69 33.61547925202319 35.0 33.0 38.5 25.0 40.0 70-71 33.28093609323436 35.0 32.0 38.0 25.0 40.0 72-73 32.9551064059979 35.0 32.0 37.0 24.0 40.0 74-75 32.60821337781 35.0 32.0 37.0 24.0 39.0 76-77 31.611511295116458 34.0 30.5 35.5 22.5 38.0 78-79 32.106897758985994 34.5 31.0 36.0 24.0 38.0 80-81 32.011873093653 35.0 31.0 35.5 24.0 37.5 82-83 31.76827704198785 34.0 31.0 35.0 23.5 37.0 84-85 31.519638255222524 34.0 31.0 35.0 23.0 36.5 86-87 31.271523552184263 34.0 31.0 35.0 22.0 36.0 88-89 31.04420420113687 34.0 31.0 35.0 20.0 36.0 90-91 30.827746265256657 34.0 30.0 35.0 20.0 35.0 92-93 30.65346305844753 34.0 30.0 35.0 20.0 35.0 94-95 30.501380936093234 34.0 30.0 35.0 19.5 35.0 96-97 30.339641736122264 34.0 29.5 35.0 19.0 35.0 98-99 30.14010144336381 34.0 29.0 35.0 18.5 35.0 100 28.26015035067713 32.0 25.0 34.0 14.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3326140963954778 1101 2 -0.2688737863850683 1101 3 -0.3622961790270338 1101 4 -0.2206566219989483 1101 5 -0.2573856408480353 1101 6 -0.28590289099807364 1101 7 -0.33903490857082375 1101 8 -0.42533855332093395 1101 9 -0.5087706223656454 1101 10-11 -0.4627812203311521 1101 12-13 -0.5391729032534087 1101 14-15 -0.7408106075437146 1101 16-17 -0.6652330592304878 1101 18-19 -0.7280238473884495 1101 20-21 -0.7203210099892132 1101 22-23 -0.831348766935271 1101 24-25 -0.7874662493974611 1101 26-27 -0.8197177164995679 1101 28-29 -0.9099056946573185 1101 30-31 -0.822278339593332 1101 32-33 -0.7028968290383091 1101 34-35 -0.7618326123830101 1101 36-37 -0.7741464456201328 1101 38-39 -0.8472988117883844 1101 40-41 -0.8583584704794163 1101 42-43 -0.8404849611631846 1101 44-45 -0.8089205667634971 1101 46-47 -0.8407831428256216 1101 48-49 -0.7717256088920905 1101 50-51 -0.8448359116917814 1101 52-53 -0.8281086352164806 1101 54-55 -0.8350622547386664 1101 56-57 -0.8953893842495191 1101 58-59 -0.8985100738141654 1101 60-61 -0.8450547332845915 1101 62-63 -0.9874110489819969 1101 64-65 -1.0171569047258018 1101 66-67 -1.0029123173359835 1101 68-69 -1.0902895904186494 1101 70-71 -0.9947408875509822 1101 72-73 -1.181986987770351 1101 74-75 -1.299108247294697 1101 76-77 -1.017278321507888 1101 78-79 -0.9125251614134342 1101 80-81 -0.8385031720397258 1101 82-83 -0.8861350927289529 1101 84-85 -0.90066438742085 1101 86-87 -1.006918019779718 1101 88-89 -0.9649775313010842 1101 90-91 -1.1015444814283661 1101 92-93 -1.1300733592132453 1101 94-95 -1.1483998279096532 1101 96-97 -1.2094042480578224 1101 98-99 -1.328096308918873 1101 100 -1.3052211342727738 1102 1 -0.2835869419624544 1102 2 -0.2588311924102804 1102 3 -0.32209388174743125 1102 4 -0.18750339547838735 1102 5 -0.2549093716810873 1102 6 -0.2956428064434391 1102 7 -0.2464311754164541 1102 8 -0.34046860453623395 1102 9 -0.4508902645933688 1102 10-11 -0.4192310725128223 1102 12-13 -0.4168681451098948 1102 14-15 -0.5612746448207346 1102 16-17 -0.5964811730759152 1102 18-19 -0.597578019982123 1102 20-21 -0.6017054377055757 1102 22-23 -0.6590254082033695 1102 24-25 -0.776798124051389 1102 26-27 -0.8058085495537455 1102 28-29 -0.7799962422066713 1102 30-31 -0.730239423344635 1102 32-33 -0.670523729134537 1102 34-35 -0.7218277488414842 1102 36-37 -0.6608613288935388 1102 38-39 -0.7387955497744372 1102 40-41 -0.8243904188015065 1102 42-43 -0.8240694231886465 1102 44-45 -0.7931477977056787 1102 46-47 -0.8316836341148175 1102 48-49 -0.8538052140475969 1102 50-51 -0.8265755732983351 1102 52-53 -0.9069371754587792 1102 54-55 -0.8230414928174596 1102 56-57 -0.8973234687695424 1102 58-59 -0.9171196959524863 1102 60-61 -0.8927218134608168 1102 62-63 -0.9178980932747649 1102 64-65 -0.9204443791655592 1102 66-67 -1.0368475538347823 1102 68-69 -1.0898489796043123 1102 70-71 -1.0334831966529805 1102 72-73 -1.0343406437228637 1102 74-75 -1.0451027045938872 1102 76-77 -0.7432584882434732 1102 78-79 -0.7279522283052628 1102 80-81 -0.6651664371640891 1102 82-83 -0.6938986022880975 1102 84-85 -0.7930388995264934 1102 86-87 -0.8951441888891054 1102 88-89 -0.7548724735690335 1102 90-91 -0.8146362312007547 1102 92-93 -1.010242476133154 1102 94-95 -0.9533049055351093 1102 96-97 -1.0121787650665865 1102 98-99 -0.9803210088786436 1102 100 -1.0733123053105302 1103 1 -0.4102148854534349 1103 2 -0.3917579904433204 1103 3 -0.3815550315105831 1103 4 -0.27252113344100337 1103 5 -0.22309497000775025 1103 6 -0.34556074264823167 1103 7 -0.3192498334714813 1103 8 -0.41882327544890785 1103 9 -0.4947774510259606 1103 10-11 -0.5284838633324256 1103 12-13 -0.5432252650220804 1103 14-15 -0.7259845890133008 1103 16-17 -0.88062553466645 1103 18-19 -0.7704440133638002 1103 20-21 -0.7273651723373078 1103 22-23 -0.8894506797654458 1103 24-25 -0.7972204897245874 1103 26-27 -0.8931122448056499 1103 28-29 -0.9279522707803167 1103 30-31 -0.8065899652496356 1103 32-33 -0.8458891602514313 1103 34-35 -0.8471054368733988 1103 36-37 -0.7566068072068006 1103 38-39 -0.7987826272324199 1103 40-41 -0.900589883398986 1103 42-43 -0.8909887513588757 1103 44-45 -0.821995385441376 1103 46-47 -0.8978064807345305 1103 48-49 -0.8587580208110808 1103 50-51 -0.821496546908989 1103 52-53 -0.8820038418406213 1103 54-55 -0.8959292862996691 1103 56-57 -0.9852549607398942 1103 58-59 -1.0446295584858376 1103 60-61 -0.9719294135761558 1103 62-63 -0.9915761215203034 1103 64-65 -1.0160400424754243 1103 66-67 -1.0765869059378659 1103 68-69 -1.12011218210273 1103 70-71 -1.0002861835364811 1103 72-73 -1.0023322356120374 1103 74-75 -1.0223579258560562 1103 76-77 -0.9881059299402892 1103 78-79 -0.8613267343316622 1103 80-81 -0.743202467985288 1103 82-83 -0.6895933359746138 1103 84-85 -0.733495020291496 1103 86-87 -0.8684503078081249 1103 88-89 -0.79154905504139 1103 90-91 -1.0058890654781472 1103 92-93 -0.874840332032953 1103 94-95 -1.0065879719752715 1103 96-97 -1.13518453219978 1103 98-99 -1.2774974740140337 1103 100 -1.2411417914536997 1104 1 -0.39579332101447307 1104 2 -0.3639927028689165 1104 3 -0.37324785373494507 1104 4 -0.1863540109857169 1104 5 -0.249695011409365 1104 6 -0.29782595183417726 1104 7 -0.36396653827792846 1104 8 -0.3693129252176135 1104 9 -0.5448808421002411 1104 10-11 -0.4912118242695982 1104 12-13 -0.6493259988840521 1104 14-15 -0.7334234103845958 1104 16-17 -0.6826665089741653 1104 18-19 -0.6885852470312841 1104 20-21 -0.7141034659509273 1104 22-23 -0.7227135076629878 1104 24-25 -0.8780284748492093 1104 26-27 -0.9352107359929391 1104 28-29 -0.8528788750235847 1104 30-31 -0.8541164552473788 1104 32-33 -0.7099921325297203 1104 34-35 -0.8431847864160531 1104 36-37 -0.8084891578628799 1104 38-39 -0.9559682047815485 1104 40-41 -0.9186395429219303 1104 42-43 -0.8203151033685998 1104 44-45 -0.9122638993700107 1104 46-47 -0.9276153432536915 1104 48-49 -0.8672385309374491 1104 50-51 -0.8403377386707973 1104 52-53 -0.8306785315631089 1104 54-55 -0.8393567182277124 1104 56-57 -1.0278567088720507 1104 58-59 -1.0902136854661038 1104 60-61 -0.9915754014775686 1104 62-63 -1.049998933135278 1104 64-65 -1.0848094788154796 1104 66-67 -1.1222570690310576 1104 68-69 -0.9512273009420653 1104 70-71 -0.946246694611844 1104 72-73 -0.9261576210625115 1104 74-75 -0.9427283313103629 1104 76-77 -0.8709815366686975 1104 78-79 -0.837014460524081 1104 80-81 -0.9307303645431126 1104 82-83 -0.8997188565800016 1104 84-85 -0.7945511344348226 1104 86-87 -0.7785338467011442 1104 88-89 -0.8199970695886201 1104 90-91 -0.8950372914482614 1104 92-93 -0.9891820979771815 1104 94-95 -1.0073355410640055 1104 96-97 -1.0403507922082156 1104 98-99 -1.0696378100380635 1104 100 -1.1832726417790198 1105 1 -0.39851910707287885 1105 2 -0.36601674509006443 1105 3 -0.35979777767518684 1105 4 -0.23466987673192108 1105 5 -0.2640334562709725 1105 6 -0.3837961536659975 1105 7 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-0.9444186319980261 1105 70-71 -0.8978194271376339 1105 72-73 -0.9776712493133957 1105 74-75 -1.0145683693844063 1105 76-77 -0.9940005519479094 1105 78-79 -0.862697219145943 1105 80-81 -0.7105019084101265 1105 82-83 -0.8428611301111388 1105 84-85 -0.802123352138981 1105 86-87 -0.8049739757139847 1105 88-89 -0.8334993751606525 1105 90-91 -0.7777123545822775 1105 92-93 -0.856378393129809 1105 94-95 -0.8675286229551276 1105 96-97 -0.9216450004563654 1105 98-99 -0.9769687698585372 1105 100 -1.077982615968299 1106 1 -0.40393005357552525 1106 2 -0.3964587072777519 1106 3 -0.4087744218425655 1106 4 -0.28153707440899467 1106 5 -0.3402751388551337 1106 6 -0.40909838568899204 1106 7 -0.3573514304818559 1106 8 -0.40943457263002614 1106 9 -0.5326866541339186 1106 10-11 -0.5222963391510262 1106 12-13 -0.5825290729367936 1106 14-15 -0.735431264192691 1106 16-17 -0.7801314302995905 1106 18-19 -0.7839379240124202 1106 20-21 -0.7502627595058797 1106 22-23 -0.7362071956163305 1106 24-25 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1.1314269011151055 2208 80-81 1.0828196799449294 2208 82-83 1.0473504815844237 2208 84-85 1.1139229018141457 2208 86-87 1.0989018890455924 2208 88-89 1.1251512620212019 2208 90-91 1.2489487848968714 2208 92-93 1.3288361744623138 2208 94-95 1.3070176933952062 2208 96-97 1.2574681701840689 2208 98-99 1.3320973156083191 2208 100 1.461718517805192 2209 1 0.7065719833796926 2209 2 0.6550669307110795 2209 3 0.6240212365157305 2209 4 0.4302862697199501 2209 5 0.49220605591956 2209 6 0.5432758130368001 2209 7 0.5786198750113343 2209 8 0.5868699489111364 2209 9 0.7944133976092331 2209 10-11 0.7434560976191165 2209 12-13 0.8154255350832642 2209 14-15 1.0115436124643438 2209 16-17 1.0085900312437843 2209 18-19 0.95485043209721 2209 20-21 0.9601240731435539 2209 22-23 0.9534979262970609 2209 24-25 0.9803728295676919 2209 26-27 0.9845675806607801 2209 28-29 1.049342684636386 2209 30-31 1.1119286172471377 2209 32-33 1.1476571161774558 2209 34-35 1.1026591760682933 2209 36-37 1.1296689828612827 2209 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0.9500719093681909 2311 34-35 0.9451943025876162 2311 36-37 1.0176763886386766 2311 38-39 1.0465817749004245 2311 40-41 1.0401673876652637 2311 42-43 1.0929743590590846 2311 44-45 1.0215201065970732 2311 46-47 1.0921714778300142 2311 48-49 1.1294247672136137 2311 50-51 1.1098556836341231 2311 52-53 1.0500901182502815 2311 54-55 1.0070695807895262 2311 56-57 1.0752346393896914 2311 58-59 1.0894927457252805 2311 60-61 1.1299639165851616 2311 62-63 1.1678151007423665 2311 64-65 1.1573975538965513 2311 66-67 1.1528080288697709 2311 68-69 1.159699054217107 2311 70-71 1.197608103651497 2311 72-73 1.256746360202122 2311 74-75 1.186080497408632 2311 76-77 1.1007917729246195 2311 78-79 1.0247568306332937 2311 80-81 1.0253633069250263 2311 82-83 1.0841336559048926 2311 84-85 1.1502969080833232 2311 86-87 1.1701451471285935 2311 88-89 1.1234432809508768 2311 90-91 1.190337736622702 2311 92-93 1.2474708941201698 2311 94-95 1.324896942654835 2311 96-97 1.362949952073329 2311 98-99 1.3980392776395867 2311 100 1.52062387528278 2312 1 0.20935573905262217 2312 2 0.21251838826429292 2312 3 0.35571562925743905 2312 4 0.2593809958426476 2312 5 0.3537074647181697 2312 6 0.43290992932543304 2312 7 0.49492846794966994 2312 8 0.3037315766335027 2312 9 0.459822443132353 2312 10-11 0.32401201257482626 2312 12-13 0.5124911242765009 2312 14-15 0.8688089772048286 2312 16-17 0.9005331155997993 2312 18-19 0.9144438614941492 2312 20-21 0.9223924477574101 2312 22-23 0.5416779913162486 2312 24-25 0.48390831904784903 2312 26-27 0.807305216529258 2312 28-29 0.9892980998117693 2312 30-31 0.9769967762808989 2312 32-33 0.8099649809863649 2312 34-35 1.0187332670222204 2312 36-37 1.0526391143083842 2312 38-39 1.028836568584289 2312 40-41 1.0672730631108536 2312 42-43 0.999665122262499 2312 44-45 0.9101388071411307 2312 46-47 1.0867059879612242 2312 48-49 1.0972983524310607 2312 50-51 1.1302117141480394 2312 52-53 1.1579921403094886 2312 54-55 1.1096913603355034 2312 56-57 1.1385244703165114 2312 58-59 1.1625342223075066 2312 60-61 1.1753278956094633 2312 62-63 1.1180164542900144 2312 64-65 1.2268036851264625 2312 66-67 1.194383130788502 2312 68-69 1.210196071433998 2312 70-71 1.015134516760689 2312 72-73 1.1483118663862726 2312 74-75 1.1714827966198484 2312 76-77 1.1051797951469844 2312 78-79 1.0357335665984237 2312 80-81 0.9329310209125232 2312 82-83 0.8991459342617247 2312 84-85 0.7419009017914568 2312 86-87 0.967039214402277 2312 88-89 0.5914681754762334 2312 90-91 0.9912050393793699 2312 92-93 1.206144340029514 2312 94-95 1.2146952125987944 2312 96-97 1.2804689657468593 2312 98-99 1.3017560429305348 2312 100 1.3300449518039237 2313 1 -0.3136294288564052 2313 2 0.03785405258730634 2313 3 0.300464585388994 2313 4 0.11425164555112133 2313 5 0.27190951775632755 2313 6 0.3728351552482536 2313 7 0.21447815185020858 2313 8 0.1746911708315011 2313 9 0.3080355620415105 2313 10-11 0.4224009181284032 2313 12-13 0.6304671446916501 2313 14-15 0.6857679864592896 2313 16-17 0.7988686360122443 2313 18-19 0.7203291001699696 2313 20-21 0.8475055674158298 2313 22-23 0.9183804831912425 2313 24-25 0.7765194473403838 2313 26-27 0.9372184516261797 2313 28-29 0.8453801885252759 2313 30-31 0.8354064313114762 2313 32-33 1.020466874947779 2313 34-35 1.0482926229022311 2313 36-37 0.9000532129979746 2313 38-39 0.7262283378809045 2313 40-41 0.8643551662929312 2313 42-43 0.9233788665496832 2313 44-45 1.0990644552360962 2313 46-47 1.1113020436228354 2313 48-49 1.132609138567794 2313 50-51 1.007550751972751 2313 52-53 0.9650081428042867 2313 54-55 1.1161389354163944 2313 56-57 1.1149779542572986 2313 58-59 1.0400575341420932 2313 60-61 1.066574915665747 2313 62-63 1.0692428140060102 2313 64-65 1.1824309699006932 2313 66-67 1.1433323704006497 2313 68-69 1.2242196601091422 2313 70-71 1.0697567334021727 2313 72-73 1.1777488982731867 2313 74-75 0.8244055636611805 2313 76-77 1.0132637652910965 2313 78-79 0.8878072289211616 2313 80-81 0.9903599460090042 2313 82-83 1.0112823242867819 2313 84-85 1.03789445191045 2313 86-87 1.1390767621453222 2313 88-89 1.1235447844779038 2313 90-91 1.1866547328136612 2313 92-93 0.9135862932965537 2313 94-95 0.9580814492182803 2313 96-97 1.2367333584196665 2313 98-99 1.3294035777577946 2313 100 1.4191300060571805 2314 1 -0.25042663675467125 2314 2 -0.09050712702968156 2314 3 0.1755451157299177 2314 4 -0.11210379681802607 2314 5 0.21092899897676887 2314 6 0.2572177304178851 2314 7 0.18446957344466597 2314 8 0.16847971537054462 2314 9 0.2589148326406985 2314 10-11 0.45878907085851495 2314 12-13 0.5886489826166326 2314 14-15 0.6386562838661618 2314 16-17 0.8505028446239891 2314 18-19 0.8530170090893989 2314 20-21 0.5022773454197207 2314 22-23 0.7102691951465232 2314 24-25 0.8969587281340168 2314 26-27 0.8823694807474141 2314 28-29 0.7596136987283444 2314 30-31 0.7380847078887243 2314 32-33 0.8231222204186253 2314 34-35 0.8631042013863421 2314 36-37 0.8081082910882955 2314 38-39 0.8242576360421268 2314 40-41 0.8522039794005778 2314 42-43 0.600923468829123 2314 44-45 0.6725341232588278 2314 46-47 0.8704344046517107 2314 48-49 0.7616806714835107 2314 50-51 0.8921748063839843 2314 52-53 0.9579608836463791 2314 54-55 1.021709054460132 2314 56-57 0.9099892249919037 2314 58-59 0.8670697067909714 2314 60-61 0.8959553478308706 2314 62-63 0.6213943138856024 2314 64-65 0.7932541966203459 2314 66-67 0.9341404779200744 2314 68-69 0.9509391577454522 2314 70-71 1.0137913881165588 2314 72-73 1.06061102611379 2314 74-75 1.030097236731308 2314 76-77 0.84288797619595 2314 78-79 0.8922118537229125 2314 80-81 0.8991228543375946 2314 82-83 0.8873365962752615 2314 84-85 0.7841060606712809 2314 86-87 0.9416120227135849 2314 88-89 0.9883092551877262 2314 90-91 1.0316250277690102 2314 92-93 1.1538025674835986 2314 94-95 1.1710411971421841 2314 96-97 1.2422319375214421 2314 98-99 1.2293374671864292 2314 100 1.270322391652357 2315 1 -0.06108464390984736 2315 2 0.13630805391065337 2315 3 0.07657334726471987 2315 4 -0.027872773243558413 2315 5 0.23751235198550802 2315 6 0.3339256887513855 2315 7 0.430213158596473 2315 8 0.40690282523132026 2315 9 0.58413217914255 2315 10-11 0.5640190679772346 2315 12-13 0.6082169708033831 2315 14-15 0.7204042174486673 2315 16-17 0.7284996877223264 2315 18-19 0.8058820436647522 2315 20-21 0.7881960433215838 2315 22-23 0.907476680269923 2315 24-25 0.8193688282184652 2315 26-27 0.9099103839084108 2315 28-29 0.9171250712777592 2315 30-31 0.8399827381141449 2315 32-33 0.8838271734177567 2315 34-35 0.918084800272247 2315 36-37 0.9209245189934805 2315 38-39 0.9570655027349417 2315 40-41 0.886601952100456 2315 42-43 0.9082498342353631 2315 44-45 0.7892278852891721 2315 46-47 0.7807776845679939 2315 48-49 0.8471424790260684 2315 50-51 0.7736079627053911 2315 52-53 0.7902097896606506 2315 54-55 0.6463314743145432 2315 56-57 0.7556381055948975 2315 58-59 0.8438219222746426 2315 60-61 0.8801571334236726 2315 62-63 0.844750546552909 2315 64-65 0.9023650418356226 2315 66-67 0.9117365930952062 2315 68-69 0.8031153114577023 2315 70-71 0.44037679203589875 2315 72-73 0.7940365545484198 2315 74-75 0.9448602665165353 2315 76-77 0.8624904171797532 2315 78-79 0.7191296927434365 2315 80-81 0.7478485635524841 2315 82-83 0.7562002592416164 2315 84-85 0.6774364618694086 2315 86-87 0.6960404389303889 2315 88-89 0.7744211638751857 2315 90-91 0.8142126216606975 2315 92-93 0.8926453136629036 2315 94-95 0.9663238026190122 2315 96-97 0.983108236598234 2315 98-99 0.9048799705579249 2315 100 0.8436772862255495 2316 1 0.49143660040177295 2316 2 0.5014090772169908 2316 3 0.4995581075356341 2316 4 0.2938677355794894 2316 5 0.3969481009724518 2316 6 0.4524055514461054 2316 7 0.48506939432457585 2316 8 0.494795935742502 2316 9 0.5158280146401992 2316 10-11 0.47797472890835024 2316 12-13 0.6320234304561865 2316 14-15 0.8303111987318559 2316 16-17 0.8156281403475774 2316 18-19 0.8075177435614123 2316 20-21 0.8462720942497057 2316 22-23 0.8946261234086563 2316 24-25 0.9326311737752562 2316 26-27 0.9753591737522953 2316 28-29 0.9462273833739587 2316 30-31 0.797652229170744 2316 32-33 0.7148154701692206 2316 34-35 0.6687454640063066 2316 36-37 0.6484101126734387 2316 38-39 0.5817360989538471 2316 40-41 0.7220863095942107 2316 42-43 0.8928553877657066 2316 44-45 0.8295563126996015 2316 46-47 0.8382901992912011 2316 48-49 0.5272122473098122 2316 50-51 0.6200374741784245 2316 52-53 0.7960778283125052 2316 54-55 0.8035195932811448 2316 56-57 0.819611044695769 2316 58-59 0.8163307430945181 2316 60-61 0.8113587176675736 2316 62-63 0.828666283532236 2316 64-65 0.8164929506631822 2316 66-67 0.829360449984506 2316 68-69 0.8286907028988679 2316 70-71 0.7377127171189599 2316 72-73 0.670888856356818 2316 74-75 0.7119451274914397 2316 76-77 0.616227376419868 2316 78-79 0.6175962788352578 2316 80-81 0.6043200618757716 2316 82-83 0.6024929356657793 2316 84-85 0.6112786507682166 2316 86-87 0.59517411863267 2316 88-89 0.4913830759078799 2316 90-91 0.6258806927094263 2316 92-93 0.6243672860015259 2316 94-95 0.5625969589089763 2316 96-97 0.6634984917590216 2316 98-99 0.7883179458835059 2316 100 0.4552074230804948 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 42.0 13 202.0 14 942.0 15 2727.0 16 6399.0 17 12735.0 18 20853.0 19 30596.0 20 42745.0 21 57728.0 22 67967.0 23 67859.0 24 67316.0 25 72552.0 26 84802.0 27 102596.0 28 126397.0 29 152952.0 30 187072.0 31 231261.0 32 291888.0 33 377693.0 34 523582.0 35 726396.0 36 1095608.0 37 1577552.0 38 1306350.0 39 205769.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.97813856422889 21.005386696500125 13.380199116829191 23.63627562244179 2 15.690605804232316 21.172458422399792 39.5348899719848 23.60204580138309 3 18.434253666200533 26.69891762833802 30.790977346600172 24.075851358861275 4 12.448952625697753 17.522014169666345 39.251322651397366 30.777710553238542 5 12.528360801135763 38.36778070523355 35.524409206257765 13.579449287372919 6 29.45666380623619 38.63008451597686 17.81751618315984 14.095735494627117 7 25.612192469067992 33.406557783768044 23.278870380358125 17.702379366805847 8 25.610356300563993 35.19224059655628 20.478721259602082 18.71868184327764 9 26.02388131274644 15.197214904128264 21.26699928042103 37.51190450270427 10-11 24.71870985673348 26.352986840467718 28.301958809967775 20.626344492831027 12-13 25.24788553152734 24.461475222064298 28.470214323974545 21.82042492243382 14-15 22.545263651771116 25.798932492075068 26.178680067556375 25.47712378859744 16-17 21.338041556737675 28.531757223562053 27.3832474632249 22.746953756475367 18-19 20.909899127826296 27.911520635754755 29.865185693905268 21.31339454251368 20-21 21.96175202980833 27.15626312057462 29.534940980597813 21.347043869019235 22-23 22.27324928550837 26.925169391467495 29.2321995321617 21.569381790862437 24-25 21.863946831696403 27.058623039531028 29.67512626840435 21.40230386036822 26-27 21.768515029156234 27.198887348392688 29.241288744838457 21.791308877612625 28-29 21.248419550898777 27.69134312049784 29.36478630043367 21.69545102816971 30-31 21.820607349368046 27.314109874620062 29.328505460857063 21.536777315154826 32-33 22.006522560970488 27.54308469080003 28.735412868700276 21.714979879529206 34-35 22.01484805156039 27.295220246949526 29.39142722733376 21.29850447415632 36-37 21.882879388678752 26.896703083964578 29.334197328292767 21.886220199063903 38-39 21.780671057668105 26.810958751269897 29.760773429232323 21.647596761829675 40-41 21.48866404882135 26.36981102660102 30.14944028352562 21.992084641052013 42-43 21.373150656946862 26.645948917274154 29.569604380362556 22.411296045416425 44-45 21.21920403446554 27.2601226226983 29.531525646531303 21.989147696304855 46-47 21.339513184494205 26.98524850987361 29.198452568423626 22.47678573720856 48-49 21.640914801309055 26.834234902871213 29.060775871743843 22.464074424075893 50-51 21.2929869677758 26.87822399108044 28.835130922054308 22.993658119089456 52-53 20.824319430752393 27.250406415326754 28.783345614733598 23.141928539187255 54-55 20.755948993087063 27.22499992775434 28.683804411985196 23.335246667173397 56-57 21.356940491068286 26.807831679887446 28.38816964827222 23.447058180772046 58-59 20.75267244767336 27.01214489900263 28.664995249997443 23.57018740332656 60-61 20.825646224501167 27.26220743346504 28.179318101086665 23.732828240947132 62-63 20.915280527074753 26.845570384455375 28.02300532414421 24.216143764325658 64-65 20.35041371506368 26.892594853893804 28.142121347277886 24.614870083764632 66-67 20.871115691042093 26.735283612143878 27.944838962262875 24.44876173455115 68-69 20.743087948001875 26.766142106259654 27.930259840012393 24.560510105726078 70-71 20.64855702536213 26.55000320692405 27.86753131255435 24.93390845515947 72-73 20.956722084155057 26.63844431620721 27.874951395637627 24.529882204000106 74-75 21.062723771606677 26.85122156233206 27.537076390454967 24.548978275606295 76-77 20.918560701540702 26.778322467545472 27.45157111965676 24.851545711257064 78-79 20.86069891546673 26.809051926570138 27.456961290633036 24.8732878673301 80-81 20.690536190687393 26.93409000799683 27.58162476731918 24.7937490339966 82-83 21.003036540998345 26.18516682490737 28.051098582555433 24.760698051538856 84-85 21.220776814456464 26.387375645697116 27.399521884622764 24.992325655223656 86-87 20.667904161181756 26.70172527621765 27.64124850243443 24.989122060166167 88-89 21.027135920179127 26.060418486440174 27.50230778266547 25.410137810715227 90-91 20.937211282087688 25.892981983464498 27.59204152267743 25.577765211770387 92-93 21.12281731936278 25.81786563158575 27.606672478208566 25.452644570842903 94-95 20.487000628490232 25.628611197878726 28.099897688887122 25.784490484743923 96-97 20.730482856799746 25.55448787325638 27.896405950207864 25.818623319736005 98-99 20.524425417245183 25.902502392309444 27.774986535382755 25.798085655062618 100 20.75386352130969 25.626257023942635 27.648504583424184 25.971374871323494 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3046.0 1 2080.0 2 1343.0 3 1806.5 4 1596.5 5 1417.5 6 2238.5 7 3813.0 8 5840.5 9 6850.5 10 5839.5 11 4760.0 12 4919.5 13 5497.5 14 6290.0 15 7370.5 16 8492.0 17 9535.5 18 10672.5 19 11960.0 20 13339.5 21 14922.0 22 17107.0 23 20140.5 24 24013.5 25 28788.0 26 34637.5 27 41408.5 28 50240.5 29 63452.0 30 80110.0 31 95984.5 32 115935.0 33 134600.5 34 149042.5 35 165006.5 36 181503.5 37 206899.0 38 222375.0 39 217723.0 40 211294.0 41 209359.0 42 216135.0 43 229416.0 44 246298.0 45 274285.5 46 300310.0 47 322243.0 48 339633.5 49 337031.5 50 322805.5 51 315767.5 52 307794.5 53 290499.0 54 273168.0 55 242914.5 56 211619.5 57 177126.0 58 142669.5 59 125057.5 60 101507.0 61 74788.5 62 56465.5 63 42695.0 64 29577.0 65 18031.5 66 11780.0 67 8552.0 68 6086.0 69 4184.5 70 2964.5 71 2193.5 72 1658.5 73 1165.5 74 864.5 75 595.5 76 362.5 77 231.5 78 154.5 79 82.0 80 55.0 81 40.0 82 21.5 83 14.5 84 10.0 85 6.5 86 3.5 87 2.0 88 3.0 89 3.0 90 2.0 91 1.0 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0506007240002661 2 0.0 3 0.0 4 0.01602020265825158 5 0.004273846011177854 6 0.0 7 1.6127720796897563E-4 8 4.43512321914683E-4 9 0.0019218867282969597 10-11 9.00464411160114E-4 12-13 3.1583453227257733E-4 14-15 6.0478952988365867E-5 16-17 8.735848764986182E-5 18-19 0.0 20-21 1.343976733074797E-5 22-23 0.0 24-25 8.735848764986182E-5 26-27 1.343976733074797E-5 28-29 0.0010415819681329676 30-31 8.131059235102523E-4 32-33 0.01401095744230476 34-35 0.023069360623228893 36-37 0.03077706718741285 38-39 0.020529244597717523 40-41 1.0079825498060978E-4 42-43 0.0016799709163434961 44-45 0.009542234804831058 46-47 0.01413191534828149 48-49 0.005805979486883123 50-51 0.010449419099656547 52-53 0.015737967544305873 54-55 0.009562394455827182 56-57 0.0031516254390603986 58-59 0.008184818304425513 60-61 0.0015858925450282606 62-63 8.9374452749474E-4 64-65 0.019178547980977356 66-67 0.044707386025733126 68-69 0.05414882257558357 70-71 0.04800684890543175 72-73 0.04033274175957466 74-75 0.02792111662962891 76-77 0.028492306741185698 78-79 0.01218986896898841 80-81 0.0020831639362659353 82-83 0.02745072477305273 84-85 0.04687790844964892 86-87 0.047509577514194073 88-89 0.05852346684174204 90-91 0.03425796692607658 92-93 0.026335224084600643 94-95 0.007432191333903627 96-97 0.003944571711574529 98-99 0.02235705295469925 100 0.018909752634362394 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7440605.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.270180339398955 #Duplication Level Percentage of deduplicated Percentage of total 1 71.84904945255589 41.86657068814826 2 15.936247105244322 18.572159855116226 3 5.676610206553404 9.923313013570189 4 2.5385464743137898 5.916862434328397 5 1.2585962893508968 3.6669316377487555 6 0.754398430594205 2.637535955909032 7 0.4698418633875047 1.9164439077412405 8 0.3157044642828704 1.4716924854172957 9 0.22163506929505053 1.1623243911622 >10 0.9407352749690142 9.008660140831878 >50 0.029310256159052295 1.142695732173216 >100 0.008329761496635994 0.7969576242105066 >500 4.2658644862619225E-4 0.16503489412879357 >1k 4.5501227903487645E-4 0.5500919025967939 >5k 2.843826743159351E-5 0.08961612871380885 >10k+ 8.531480229478053E-5 1.1131092082033964 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28035 0.3767838771175194 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25431 0.34178672298825163 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13706 0.18420545103523167 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.343976733074797E-5 0.0 2 0.0 0.0 0.0 2.687953466149594E-5 0.0 3 0.0 0.0 0.0 2.687953466149594E-5 0.0 4 0.0 0.0 0.0 2.687953466149594E-5 0.0 5 0.0 2.687953466149594E-5 0.0 2.687953466149594E-5 2.687953466149594E-5 6 0.0 2.687953466149594E-5 0.0 2.687953466149594E-5 2.687953466149594E-5 7 0.0 2.687953466149594E-5 0.0 2.687953466149594E-5 2.687953466149594E-5 8 0.0 2.687953466149594E-5 0.0 2.687953466149594E-5 2.687953466149594E-5 9 0.0 2.687953466149594E-5 0.0 6.719883665373985E-5 2.687953466149594E-5 10-11 0.0 2.687953466149594E-5 0.0 9.407837131523579E-5 4.031930199224391E-5 12-13 0.0 5.375906932299188E-5 0.0 1.343976733074797E-4 1.5455732430360165E-4 14-15 0.0 6.047895298836586E-5 0.0 1.5455732430360165E-4 1.6127720796897565E-4 16-17 0.0 6.719883665373985E-5 0.0 2.0831639362659353E-4 1.6127720796897565E-4 18-19 0.0 6.719883665373985E-5 0.0 2.822351139457074E-4 1.6799709163434963E-4 20-21 0.0 6.719883665373985E-5 0.0 5.980696462182847E-4 2.7551523028033337E-4 22-23 0.0 6.719883665373985E-5 0.0 0.002163802540250423 3.023947649418293E-4 24-25 0.0 6.719883665373985E-5 0.0 0.005342307513972318 3.091146486072033E-4 26-27 0.0 6.719883665373985E-5 0.0 0.01218986896898841 3.3599418326869926E-4 28-29 0.0 6.719883665373985E-5 0.0 0.03882076793486551 3.3599418326869926E-4 30-31 0.0 6.719883665373985E-5 0.0 0.12710659953054892 3.3599418326869926E-4 32-33 0.0 6.719883665373985E-5 0.0 0.3160226890152078 3.494339505994472E-4 34-35 0.0 8.063860398448781E-5 0.0 0.6134245803936642 3.7631348526094316E-4 36-37 0.0 1.0079825498060977E-4 0.0 1.0760482514526708 3.7631348526094316E-4 38-39 0.0 1.0751813864598376E-4 0.0 1.6613635584740756 4.031930199224391E-4 40-41 0.0 1.0751813864598376E-4 0.0 2.2155926836594606 4.031930199224391E-4 42-43 0.0 1.0751813864598376E-4 0.0 2.7615563519364352 4.031930199224391E-4 44-45 0.0 1.1423802231135774E-4 0.0 3.431595952210875 4.2335267091856105E-4 46-47 0.0 1.2095790597673173E-4 0.0 4.2185077691935 4.50232205580057E-4 48-49 0.0 1.2095790597673173E-4 0.0 5.017004665615229 4.56952089245431E-4 50-51 0.0 1.2095790597673173E-4 0.0 5.789777847365906 4.7039185657617893E-4 52-53 0.0 1.2767778964210572E-4 0.0 6.498369420228597 5.107111585684229E-4 54-55 0.0 1.8815674263047158E-4 0.0 7.2618019636844044 5.107111585684229E-4 56-57 0.0 2.150362772919675E-4 0.0 8.147160076364758 5.174310422337968E-4 58-59 0.0 2.284760446227155E-4 0.0 9.112552003499715 5.241509258991708E-4 60-61 0.0 2.3519592828808946E-4 0.0 10.043632204639273 5.443105768952927E-4 62-63 0.0 2.4191581195346347E-4 0.0 10.92939351033955 5.644702278914147E-4 64-65 0.0 2.4191581195346347E-4 0.0 11.820060331115549 5.644702278914147E-4 66-67 0.0 2.8223511394570735E-4 0.0 12.790048658677621 5.644702278914147E-4 68-69 0.0 2.889549976110813E-4 0.0 13.823882600944412 5.644702278914147E-4 70-71 0.0 3.091146486072033E-4 0.0 14.870638879499719 5.644702278914147E-4 72-73 0.0 3.225544159379513E-4 0.0 15.880214041734508 6.316690645451546E-4 74-75 0.0 3.225544159379513E-4 0.0 16.83225356002637 6.988679011988944E-4 76-77 0.0 3.225544159379513E-4 0.0 17.847708620468364 7.055877848642683E-4 78-79 0.0 3.225544159379513E-4 0.0 18.904202010454796 7.257474358603904E-4 80-81 0.0 3.225544159379513E-4 0.0 19.98133216317759 7.459070868565123E-4 82-83 0.0 3.225544159379513E-4 0.0 21.034136337031732 8.063860398448782E-4 84-85 0.0 3.225544159379513E-4 0.0 22.021824838168403 8.063860398448782E-4 86-87 0.0 3.225544159379513E-4 0.0 23.007584732693108 8.198258071756262E-4 88 0.0 3.225544159379513E-4 0.0 23.765715287936935 8.332655745063741E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14020 0.0 58.016907 1 GTATCAA 25540 0.0 40.684395 1 GTGGTAT 4070 0.0 37.659943 1 GTACTAG 4110 0.0 36.835835 1 CAACGCA 30500 0.0 33.462368 5 ATCAACG 30515 0.0 33.35913 3 TATCAAC 32015 0.0 32.397587 2 AACGCAG 33240 0.0 31.311054 6 TCAACGC 33790 0.0 30.376244 4 ACGCAGA 36615 0.0 27.847466 7 CGCAGAG 37170 0.0 27.3811 8 CTGTGCG 5255 0.0 27.003263 9 TGGTATC 5725 0.0 26.84331 2 TAGTACT 5565 0.0 25.926437 4 GCAGAGT 39675 0.0 25.178413 9 GCATCGT 5545 0.0 24.065882 8 CTAACGC 2230 0.0 24.025347 3 GTACATG 27450 0.0 23.962484 1 CTAGTAC 5970 0.0 23.931442 3 TACATGG 27940 0.0 23.464518 2 >>END_MODULE