FastQCFastQC Report
Mon 6 Jun 2016
ERR1211456_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211456_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729782
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82780.478557413593158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69930.40427059594792863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40510.234191360529824No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22350.12920703302497077No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21310.12319471470971487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31750.057.612111
GTATCAA60350.042.1834681
TACCTGG15000.039.864312
GTGGTAT11500.036.8952521
ATCAACG72700.034.4019173
TATCAAC74450.033.8345872
CAACGCA74050.033.647775
ACCTGGG17250.033.5213473
ATAACGC2150.032.798823
TCAACGC76800.032.6256264
AACGCAG78800.031.7994046
TGGTATC13650.031.3892232
TAACGCA2400.029.3814284
GTACATG55850.029.1220341
ACGCAGA86400.028.7301677
TACATGG57200.027.9868952
CGCAGAG88800.027.7419078
ACATGGG57700.026.4796943
GTATAAC4350.026.0104471
GCAGAGT94800.025.885419
Produced by FastQC (version 0.11.5)