##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211456_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1729782 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.374003198090858 31.0 31.0 34.0 29.0 34.0 2 31.418238830095355 31.0 31.0 34.0 28.0 34.0 3 31.483597355042427 31.0 31.0 34.0 28.0 34.0 4 35.11928555158974 37.0 35.0 37.0 32.0 37.0 5 35.16641461178345 37.0 35.0 37.0 32.0 37.0 6 35.23929315948484 37.0 35.0 37.0 32.0 37.0 7 35.166551623268134 37.0 35.0 37.0 32.0 37.0 8 35.18019785152117 37.0 35.0 37.0 32.0 37.0 9 36.74882846508982 39.0 37.0 39.0 32.0 39.0 10-11 36.67590309067848 39.0 37.0 39.0 32.0 39.0 12-13 36.58281043507216 39.0 37.0 39.0 32.0 39.0 14-15 37.76079644718236 40.0 37.0 41.0 32.0 41.0 16-17 37.744131919513556 40.0 37.0 41.0 32.0 41.0 18-19 37.751232814308395 40.0 37.0 41.0 32.0 41.0 20-21 37.74289621466751 40.0 37.0 41.0 32.0 41.0 22-23 37.613178134585745 40.0 37.0 41.0 32.0 41.0 24-25 37.52013259474315 40.0 37.0 41.0 32.0 41.0 26-27 37.37004345056198 40.0 36.5 41.0 31.0 41.0 28-29 37.226702266528385 40.0 36.0 41.0 31.0 41.0 30-31 37.04703396150498 39.0 36.0 41.0 30.5 41.0 32-33 36.81811176206019 39.0 35.5 41.0 30.0 41.0 34-35 36.681795740734955 39.0 35.0 41.0 30.0 41.0 36-37 36.53913932507102 39.0 35.0 41.0 30.0 41.0 38-39 36.368206224830644 39.0 35.0 40.5 29.5 41.0 40-41 36.17903094147124 39.0 35.0 40.0 29.0 41.0 42-43 35.97117787096871 38.0 35.0 40.0 28.0 41.0 44-45 35.81639420458763 38.0 35.0 40.0 27.0 41.0 46-47 35.56966802753179 38.0 34.5 40.0 26.5 41.0 48-49 35.29033485144371 38.0 34.0 40.0 25.5 41.0 50-51 34.604083346918856 37.5 33.5 39.5 24.5 40.5 52-53 34.897169123045565 38.0 33.5 39.5 25.5 40.5 54-55 35.29422262458506 38.0 34.0 40.0 26.0 41.0 56-57 35.209299206489604 38.0 34.0 40.0 26.0 41.0 58-59 34.99460481147335 38.0 34.0 40.0 25.5 41.0 60-61 34.76117481856095 37.5 34.0 40.0 25.0 41.0 62-63 34.45513567605629 37.0 33.0 40.0 24.5 41.0 64-65 34.24170213356365 37.0 33.0 40.0 24.5 41.0 66-67 34.031994494103884 36.0 33.0 39.5 23.5 41.0 68-69 33.68344074571247 36.0 33.0 39.0 23.0 41.0 70-71 33.29598296201486 35.0 32.5 39.0 22.5 41.0 72-73 32.903790477644 35.0 32.0 38.0 21.5 40.0 74-75 32.51474318729181 35.0 32.0 37.0 20.5 39.5 76-77 32.1171803730181 35.0 31.0 37.0 20.0 39.0 78-79 31.71178911562266 35.0 31.0 36.0 19.5 39.0 80-81 31.31468416251296 35.0 30.5 36.0 18.0 37.5 82-83 30.937168094014158 34.5 30.0 35.0 17.5 37.0 84-85 30.596454929002615 34.0 30.0 35.0 16.0 37.0 86-87 30.24628623722527 34.0 29.0 35.0 14.5 36.0 88-89 29.932226719898807 34.0 29.0 35.0 11.5 36.0 90-91 29.613813185707794 34.0 29.0 35.0 10.0 36.0 92-93 29.29884257091356 34.0 27.5 35.0 10.0 35.0 94-95 28.99527454904722 34.0 27.0 35.0 10.0 35.0 96-97 28.686032690824625 34.0 26.0 35.0 9.0 35.0 98-99 28.350117529260913 33.5 25.0 35.0 8.0 35.0 100 27.093558610275746 32.0 23.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3491144347758919 1101 2 -0.34967468518267 1101 3 -0.37479203580985754 1101 4 -0.40108308858407327 1101 5 -0.40878909368413474 1101 6 -0.4017814103126085 1101 7 -0.42899942817334136 1101 8 -0.47425075354423996 1101 9 -0.7928878950112406 1101 10-11 -0.6017914289380784 1101 12-13 -0.528851512666229 1101 14-15 -0.7723537378150809 1101 16-17 -0.8972810068830768 1101 18-19 -0.7615303823111788 1101 20-21 -0.9175972533607251 1101 22-23 -0.89030749954334 1101 24-25 -0.846958928692338 1101 26-27 -0.8405015680294952 1101 28-29 -0.8942652207855133 1101 30-31 -1.1484846907879316 1101 32-33 -0.924365577138957 1101 34-35 -1.0424325153276826 1101 36-37 -1.0017351643434864 1101 38-39 -0.9535731029824603 1101 40-41 -1.238598916079333 1101 42-43 -1.1465726851091702 1101 44-45 -0.9953444020717868 1101 46-47 -0.9160694665834228 1101 48-49 -1.1363138942178992 1101 50-51 -1.015036663788429 1101 52-53 -0.8231114380419129 1101 54-55 -0.8628213092340289 1101 56-57 -0.9520172084250618 1101 58-59 -0.811589105181433 1101 60-61 -0.8189609570462295 1101 62-63 -0.9306414837450987 1101 64-65 -0.762983332288222 1101 66-67 -0.837252224238533 1101 68-69 -0.9665638601961035 1101 70-71 -1.168774916608264 1101 72-73 -1.1665611220911067 1101 74-75 -1.2777424292309192 1101 76-77 -1.0497267501522565 1101 78-79 -1.1110571718148314 1101 80-81 -1.231489499658661 1101 82-83 -1.422490050687788 1101 84-85 -1.5302425728603168 1101 86-87 -1.617152247322199 1101 88-89 -1.6056471987630196 1101 90-91 -1.710643017986321 1101 92-93 -1.7926959978855592 1101 94-95 -1.946314602804069 1101 96-97 -2.074109376816395 1101 98-99 -2.3371005937069 1101 100 -2.1438893324166663 1102 1 -0.364534153762321 1102 2 -0.3961019699190693 1102 3 -0.4315282982264357 1102 4 -0.4143943479983676 1102 5 -0.3214818830612032 1102 6 -0.36451365824961357 1102 7 -0.27366909140310725 1102 8 -0.35793978578016805 1102 9 -0.37414250565819174 1102 10-11 -0.4741717236763634 1102 12-13 -0.5750236034552287 1102 14-15 -0.8912762617904448 1102 16-17 -0.7254998817994434 1102 18-19 -0.7896051477043002 1102 20-21 -0.7128969668572651 1102 22-23 -0.9391516210757018 1102 24-25 -0.7817553739907623 1102 26-27 -1.0975311962612295 1102 28-29 -1.0073371554558577 1102 30-31 -0.9831263041283549 1102 32-33 -0.9986746234427173 1102 34-35 -1.0774961637004523 1102 36-37 -0.9601930208858889 1102 38-39 -1.0673630118846518 1102 40-41 -1.192836851195736 1102 42-43 -1.1232934214402093 1102 44-45 -1.0825606864479198 1102 46-47 -1.120169296740059 1102 48-49 -1.3950942815980198 1102 50-51 -1.143435246711455 1102 52-53 -0.9639939373127859 1102 54-55 -0.9399036060653359 1102 56-57 -0.946116867022738 1102 58-59 -0.9727442974132359 1102 60-61 -1.1859380024578812 1102 62-63 -1.1139041919747754 1102 64-65 -1.0651466907263512 1102 66-67 -1.0314016349335233 1102 68-69 -1.039753055413044 1102 70-71 -0.8649152689903588 1102 72-73 -0.8507991429869861 1102 74-75 -1.0696332662670684 1102 76-77 -1.2729331012648188 1102 78-79 -1.2786063663455316 1102 80-81 -1.5188953566814227 1102 82-83 -1.615123738004435 1102 84-85 -1.5803914002548503 1102 86-87 -1.6996296444297165 1102 88-89 -1.812440075635159 1102 90-91 -1.8064698463758972 1102 92-93 -2.096169208954546 1102 94-95 -2.3680854343503306 1102 96-97 -2.2483107698751184 1102 98-99 -2.563710674511171 1102 100 -2.310528835581927 1103 1 -0.4379998044209543 1103 2 -0.3486862926788312 1103 3 -0.47961400069810267 1103 4 -0.24177593327468827 1103 5 -0.33330732207962654 1103 6 -0.41985487323994874 1103 7 -0.44531326918763625 1103 8 -0.39967754769336494 1103 9 -0.35964709203997103 1103 10-11 -0.38474592854881706 1103 12-13 -0.6390660749538384 1103 14-15 -0.8225490987650375 1103 16-17 -0.8087226694081906 1103 18-19 -0.8709140597149698 1103 20-21 -0.7285393978505752 1103 22-23 -0.8448858640906494 1103 24-25 -0.6101209006019062 1103 26-27 -0.6156087976721665 1103 28-29 -0.7996550714579911 1103 30-31 -0.9064744472133341 1103 32-33 -0.7919093938704691 1103 34-35 -0.754569528931718 1103 36-37 -1.0531328620713438 1103 38-39 -0.9242936381135252 1103 40-41 -0.9157909114304985 1103 42-43 -0.9184587337107857 1103 44-45 -1.06460333515723 1103 46-47 -0.9074685634252972 1103 48-49 -1.0813967380759166 1103 50-51 -1.0031744516260943 1103 52-53 -0.8705317464821789 1103 54-55 -0.8728095240589724 1103 56-57 -0.9489267114992543 1103 58-59 -0.8363298184548995 1103 60-61 -0.974446943159812 1103 62-63 -0.8298046333138132 1103 64-65 -0.9409907621844624 1103 66-67 -0.8570016197440324 1103 68-69 -0.8961206157279733 1103 70-71 -0.9147239236235762 1103 72-73 -0.7903968746899821 1103 74-75 -0.943679281979751 1103 76-77 -1.049263025401757 1103 78-79 -1.0019114725453733 1103 80-81 -0.9433934706532234 1103 82-83 -0.9993230831686901 1103 84-85 -1.0106051923107486 1103 86-87 -1.274069321062374 1103 88-89 -1.182483450064499 1103 90-91 -1.3476034041761231 1103 92-93 -1.3053470356762311 1103 94-95 -1.5289151179870366 1103 96-97 -1.6402550666439737 1103 98-99 -1.3790856017281996 1103 100 -1.4530716404054367 1104 1 -0.4500547291661654 1104 2 -0.4239524592481594 1104 3 -0.3939097559386333 1104 4 -0.1610347374403176 1104 5 -0.2686941390283266 1104 6 -0.21448754946864312 1104 7 -0.31950516194735457 1104 8 -0.4147044867082883 1104 9 -0.5204569564473474 1104 10-11 -0.43120972458596185 1104 12-13 -0.5081476454314071 1104 14-15 -0.5524579789060127 1104 16-17 -0.5250071838359318 1104 18-19 -0.7035034413691434 1104 20-21 -0.6888065630976072 1104 22-23 -0.5692748376775185 1104 24-25 -0.6198169233363586 1104 26-27 -0.4528211721737705 1104 28-29 -0.5823990585333263 1104 30-31 -0.7509177539236944 1104 32-33 -0.7638648806563211 1104 34-35 -0.8025079547628238 1104 36-37 -0.675453182255005 1104 38-39 -0.666823149671643 1104 40-41 -0.9206237530098704 1104 42-43 -0.7682228435097116 1104 44-45 -0.6485611580904092 1104 46-47 -0.6040816554578683 1104 48-49 -0.7719172640630276 1104 50-51 -0.8251620477962334 1104 52-53 -0.7455611379461331 1104 54-55 -0.7511977593828405 1104 56-57 -0.8130762047019147 1104 58-59 -0.8312940809630192 1104 60-61 -1.0960997926780394 1104 62-63 -0.980933012700099 1104 64-65 -1.000718641473405 1104 66-67 -1.0776423915828417 1104 68-69 -0.9542152145250071 1104 70-71 -0.9953880063697156 1104 72-73 -0.90392377451629 1104 74-75 -0.8054869391829982 1104 76-77 -1.1312057948487961 1104 78-79 -0.9988832036457147 1104 80-81 -0.9429516505820779 1104 82-83 -0.99867394266359 1104 84-85 -1.1759179554117551 1104 86-87 -1.1220516128633555 1104 88-89 -1.1817698379610313 1104 90-91 -1.2947259482506759 1104 92-93 -1.2532059465360277 1104 94-95 -1.3567146391274 1104 96-97 -1.3313817520706124 1104 98-99 -1.3583124734904004 1104 100 -1.2912378153181443 1105 1 -0.5826058844535567 1105 2 -0.4478604727291966 1105 3 -0.629034361315675 1105 4 -0.3481945185098212 1105 5 -0.3993778876302514 1105 6 -0.36965466128736324 1105 7 -0.3382635472977569 1105 8 -0.2822240101599931 1105 9 -0.3027242807982873 1105 10-11 -0.42392485454804074 1105 12-13 -0.6073502156575543 1105 14-15 -0.6974343325278696 1105 16-17 -0.6577393758936196 1105 18-19 -0.6488160769917144 1105 20-21 -0.6656456362885379 1105 22-23 -0.6658405949444131 1105 24-25 -0.8121684917843837 1105 26-27 -0.9427692914993315 1105 28-29 -0.8799050910257051 1105 30-31 -0.7801620122061266 1105 32-33 -0.8193495508098039 1105 34-35 -0.8133397815230339 1105 36-37 -0.8652293166487581 1105 38-39 -0.9764319862499917 1105 40-41 -1.03084662663381 1105 42-43 -1.0079186273369274 1105 44-45 -1.2402755019065168 1105 46-47 -1.2757119535733494 1105 48-49 -1.2450847627548498 1105 50-51 -1.389438781099308 1105 52-53 -1.1203425918449952 1105 54-55 -0.9586900777198721 1105 56-57 -1.095953598169217 1105 58-59 -0.8591780835124183 1105 60-61 -1.053087440018409 1105 62-63 -1.1388547353765617 1105 64-65 -0.8753463381152855 1105 66-67 -0.8508070383255415 1105 68-69 -0.966750770890684 1105 70-71 -0.9139846986047218 1105 72-73 -0.9673151636139075 1105 74-75 -1.0253071175910158 1105 76-77 -1.1911097461623044 1105 78-79 -1.020907070739927 1105 80-81 -1.01266506689473 1105 82-83 -1.2545749973887723 1105 84-85 -1.0559352159038298 1105 86-87 -1.00153072295603 1105 88-89 -1.0021113030692916 1105 90-91 -1.2680174447303791 1105 92-93 -1.321589486573675 1105 94-95 -1.3824324003225392 1105 96-97 -1.3545147793774035 1105 98-99 -1.369639051424251 1105 100 -1.3259348763225773 1106 1 -0.34576564507371543 1106 2 -0.4487309021423478 1106 3 -0.5419530302246152 1106 4 -0.41033620577182006 1106 5 -0.4343623338949456 1106 6 -0.5557851403016727 1106 7 -0.47836860209524446 1106 8 -0.43759918599887726 1106 9 -0.6739792700001814 1106 10-11 -0.731386220918516 1106 12-13 -0.528718356765836 1106 14-15 -0.690653052792932 1106 16-17 -0.6778977298027016 1106 18-19 -0.9124114440508606 1106 20-21 -0.7147949306021886 1106 22-23 -0.6149506376706668 1106 24-25 -0.7044072150814529 1106 26-27 -0.6620891030133578 1106 28-29 -0.8703840348211145 1106 30-31 -0.7656383372703814 1106 32-33 -0.7964108761267283 1106 34-35 -0.9306712768479102 1106 36-37 -0.858475654048668 1106 38-39 -1.0593652628604744 1106 40-41 -0.9540611984526564 1106 42-43 -0.8331464295261597 1106 44-45 -0.9527903338748587 1106 46-47 -0.7892776469025051 1106 48-49 -0.9110163435557297 1106 50-51 -0.8674294915569547 1106 52-53 -0.9974500632397181 1106 54-55 -0.9456982723701444 1106 56-57 -0.9032659103580158 1106 58-59 -0.8556231118993054 1106 60-61 -0.770555336568151 1106 62-63 -0.71651157230189 1106 64-65 -0.7483733288700165 1106 66-67 -0.8432893016613932 1106 68-69 -0.8543678352245081 1106 70-71 -0.8731941904376868 1106 72-73 -0.7914512075788238 1106 74-75 -0.7643823862313077 1106 76-77 -1.0017944826615377 1106 78-79 -0.7924412868030686 1106 80-81 -0.9934952497201657 1106 82-83 -0.8977894281839518 1106 84-85 -0.866737276259606 1106 86-87 -0.8889648482547763 1106 88-89 -1.0283481687712772 1106 90-91 -1.1392937833485774 1106 92-93 -1.271370958649225 1106 94-95 -1.3173977692189034 1106 96-97 -1.2156754119939066 1106 98-99 -1.5126995313826335 1106 100 -1.2438516590399686 1107 1 -0.4639014531296226 1107 2 -0.37676575058285167 1107 3 -0.3537616204597569 1107 4 -0.2823153159193339 1107 5 -0.2588833704626907 1107 6 -0.18766828140423542 1107 7 -0.15296202069603737 1107 8 -0.1760272538676375 1107 9 -0.37733375884944564 1107 10-11 -0.44387701838166294 1107 12-13 -0.3521825556141849 1107 14-15 -0.5005974961116806 1107 16-17 -0.8214539277534882 1107 18-19 -0.674420332519496 1107 20-21 -0.6678891968494938 1107 22-23 -0.6939440508681329 1107 24-25 -0.6930829353183228 1107 26-27 -0.660107508837541 1107 28-29 -0.6959984941171982 1107 30-31 -0.7800044260064709 1107 32-33 -0.7356987104668065 1107 34-35 -0.7480950092992984 1107 36-37 -0.7447640054568723 1107 38-39 -0.681226925748561 1107 40-41 -0.7942548776137599 1107 42-43 -0.8492258223726097 1107 44-45 -0.6871782910655213 1107 46-47 -0.5041436557144223 1107 48-49 -0.662793249296989 1107 50-51 -0.9147500430262525 1107 52-53 -0.5944301677490174 1107 54-55 -0.6329827491444817 1107 56-57 -0.4731093190151938 1107 58-59 -0.6658484155173525 1107 60-61 -0.7454645869844683 1107 62-63 -0.6755425536131412 1107 64-65 -0.6554674810471468 1107 66-67 -0.5896184181503088 1107 68-69 -0.539406180066166 1107 70-71 -0.6309464600215478 1107 72-73 -0.5174602596481108 1107 74-75 -0.5022534238872254 1107 76-77 -0.6852457885775038 1107 78-79 -0.5174203301594922 1107 80-81 -0.5377265255125927 1107 82-83 -0.6736013714820679 1107 84-85 -0.6712311160945674 1107 86-87 -0.7953922151922868 1107 88-89 -0.7943193075143888 1107 90-91 -0.8734140133200654 1107 92-93 -0.952018914804249 1107 94-95 -0.8099935262584275 1107 96-97 -0.9089806555450011 1107 98-99 -0.8555438413443355 1107 100 -0.9511937262468209 1108 1 -0.34613301328639423 1108 2 -0.4321585550709486 1108 3 -0.5295622305185113 1108 4 -0.20019009627903728 1108 5 -0.13962218666592463 1108 6 -0.2299688303392884 1108 7 -0.3708186848735693 1108 8 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1.148231595140338 2311 38-39 1.1465125004605028 2311 40-41 1.178820605747724 2311 42-43 1.0851939141043303 2311 44-45 1.2311509406104264 2311 46-47 1.3773559702658815 2311 48-49 1.5143009393375948 2311 50-51 1.5087485328807517 2311 52-53 1.2970420913850589 2311 54-55 1.2192635438945914 2311 56-57 1.175273421063487 2311 58-59 1.1184884913734905 2311 60-61 1.1672545024782224 2311 62-63 1.0966642912229077 2311 64-65 1.1095064784827642 2311 66-67 1.1264330918048628 2311 68-69 1.2328023495432632 2311 70-71 1.2833287474046884 2311 72-73 1.2974857632758372 2311 74-75 1.226422246208415 2311 76-77 1.195508554921446 2311 78-79 1.0347157480678995 2311 80-81 1.0837522215306272 2311 82-83 1.334502388503278 2311 84-85 1.3279672380962637 2311 86-87 1.1851601392938385 2311 88-89 1.265848544926417 2311 90-91 1.2602431947224915 2311 92-93 1.3282552376475252 2311 94-95 1.495009423626616 2311 96-97 1.4436759563940988 2311 98-99 1.5832998037210864 2311 100 1.7321904872427645 2312 1 0.6485516593234912 2312 2 0.5916312178141183 2312 3 0.7700655033673662 2312 4 0.5695090359050141 2312 5 0.5029938914145688 2312 6 0.4587693650334117 2312 7 0.5849187171846992 2312 8 0.5676669898266127 2312 9 0.7408393593236724 2312 10-11 0.7714014468968102 2312 12-13 0.8309346275030052 2312 14-15 0.9624947169805367 2312 16-17 1.040556434256871 2312 18-19 1.009975601876441 2312 20-21 1.0742677953074917 2312 22-23 1.0779404710163831 2312 24-25 1.033000290764896 2312 26-27 1.0289524302223896 2312 28-29 1.1079235598048527 2312 30-31 1.012378616376573 2312 32-33 1.0826801079120116 2312 34-35 1.08105556985128 2312 36-37 1.0897380790452118 2312 38-39 1.1402621457405075 2312 40-41 1.1028812695223849 2312 42-43 1.1198558967091117 2312 44-45 1.011806407919174 2312 46-47 0.9988675972968366 2312 48-49 1.038099951596216 2312 50-51 0.9549108266346238 2312 52-53 0.9226693493504925 2312 54-55 0.804806688644959 2312 56-57 0.6905460946402258 2312 58-59 0.6742114245424844 2312 60-61 0.7815239400040568 2312 62-63 0.8833515052809204 2312 64-65 0.7747951992155393 2312 66-67 0.7243823958505047 2312 68-69 0.6894517987918078 2312 70-71 0.7378014087263196 2312 72-73 0.7759457837579404 2312 74-75 0.742089820456016 2312 76-77 0.7055268521951419 2312 78-79 0.6980121352729718 2312 80-81 0.7206389828529147 2312 82-83 1.0382890904083943 2312 84-85 0.9961120512486019 2312 86-87 1.072992653192582 2312 88-89 1.1610398978448124 2312 90-91 1.2050325651265155 2312 92-93 1.2619395491542136 2312 94-95 1.401550435285536 2312 96-97 1.231239234674888 2312 98-99 1.2587627729622781 2312 100 1.376161101108007 2313 1 0.5706052919034406 2313 2 0.562417793863851 2313 3 0.6653028459731267 2313 4 0.5389521632428824 2313 5 0.5924254282145256 2313 6 0.5808651450239282 2313 7 0.6387356920543041 2313 8 0.6081021618407263 2313 9 0.7261404498879216 2313 10-11 0.6375570762103351 2313 12-13 0.8113755947860781 2313 14-15 1.1043339246092145 2313 16-17 1.0203256771840117 2313 18-19 1.0856828881100498 2313 20-21 1.0502306002176667 2313 22-23 1.024492285989254 2313 24-25 1.0661518633901537 2313 26-27 1.0821240228676743 2313 28-29 1.0958720345954305 2313 30-31 1.1364368851716833 2313 32-33 1.2021548458078044 2313 34-35 1.2437709167771587 2313 36-37 1.2394667550896727 2313 38-39 1.1924459084505799 2313 40-41 1.290977260249953 2313 42-43 1.2586921013347876 2313 44-45 1.1573203429068002 2313 46-47 1.1661664751220258 2313 48-49 1.340507622424937 2313 50-51 1.4326122122307439 2313 52-53 1.2524596659829186 2313 54-55 1.1380083113202701 2313 56-57 1.1198174712800153 2313 58-59 1.0472028370075819 2313 60-61 1.0095019179820355 2313 62-63 1.203105469771721 2313 64-65 1.1248821282498298 2313 66-67 1.145211119310808 2313 68-69 1.0533551758530848 2313 70-71 1.0634823528283164 2313 72-73 1.2391853391027539 2313 74-75 1.2092806955416293 2313 76-77 1.375538969575679 2313 78-79 1.3123238153741248 2313 80-81 1.261179523393455 2313 82-83 1.3336238989010987 2313 84-85 1.3984354272561887 2313 86-87 1.5811455429244177 2313 88-89 1.7184525736785439 2313 90-91 1.6495692070315755 2313 92-93 1.7628351816287946 2313 94-95 1.8510262268665905 2313 96-97 1.962259728853276 2313 98-99 2.05980065294753 2313 100 2.0538387787856855 2314 1 0.41345697062802955 2314 2 0.442190573690052 2314 3 0.5897675156744988 2314 4 0.4290070065678009 2314 5 0.427409607275429 2314 6 0.4581053370615038 2314 7 0.4700941294263288 2314 8 0.44438300018205723 2314 9 0.7074594351752594 2314 10-11 0.6467504546769405 2314 12-13 0.430515963309567 2314 14-15 0.7740625680602236 2314 16-17 0.8222291170895346 2314 18-19 0.8873430611675346 2314 20-21 0.8639459461504586 2314 22-23 0.7642437390016283 2314 24-25 0.6375556677949064 2314 26-27 0.8338538758923306 2314 28-29 0.8399694045863271 2314 30-31 0.8019616987339404 2314 32-33 0.8109033770970981 2314 34-35 0.8792055806644115 2314 36-37 0.8398098037423622 2314 38-39 0.9397842391243643 2314 40-41 1.0279519419347665 2314 42-43 0.9809747487656111 2314 44-45 1.0258799304508912 2314 46-47 1.010444181130545 2314 48-49 0.952225773890234 2314 50-51 0.9548434562192583 2314 52-53 0.7950844178747971 2314 54-55 0.7745129826625217 2314 56-57 0.747977619930623 2314 58-59 0.8345267664672988 2314 60-61 0.7836474990391693 2314 62-63 0.8170973584322851 2314 64-65 0.8151383456485846 2314 66-67 0.8301456022662066 2314 68-69 0.9626047245546943 2314 70-71 0.789395898334341 2314 72-73 0.8916565888114221 2314 74-75 0.9284125617570069 2314 76-77 0.9558041957542613 2314 78-79 0.9848587934342738 2314 80-81 0.9724405669406231 2314 82-83 0.8994229405465965 2314 84-85 0.9686104075072279 2314 86-87 1.26056773431527 2314 88-89 1.4020512088174613 2314 90-91 1.4951756565020844 2314 92-93 1.5921600548217967 2314 94-95 1.600223492102625 2314 96-97 1.7770649056818044 2314 98-99 1.9251751979537275 2314 100 1.7327366229807737 2315 1 0.5189051193062326 2315 2 0.5589042158238584 2315 3 0.627271606702049 2315 4 0.5068133040398806 2315 5 0.5216979544640097 2315 6 -0.22488589980439855 2315 7 0.33862103517608233 2315 8 0.48745655884189176 2315 9 0.7580541339293418 2315 10-11 0.7275445914491527 2315 12-13 0.7650824073804827 2315 14-15 0.9061322775819605 2315 16-17 0.7872656023644495 2315 18-19 0.9564950918027861 2315 20-21 0.9675617227403208 2315 22-23 0.832868557244332 2315 24-25 0.9223912067865072 2315 26-27 1.1436533582738662 2315 28-29 1.163929002131276 2315 30-31 1.207807621167376 2315 32-33 1.2398951596601506 2315 34-35 1.1650670801884573 2315 36-37 1.1605670027601036 2315 38-39 1.2606023357662863 2315 40-41 1.2173661203986015 2315 42-43 1.197595649601375 2315 44-45 1.3329292670523571 2315 46-47 1.2330736475516915 2315 48-49 1.3009230750481535 2315 50-51 1.2601002253247273 2315 52-53 1.1688346840892692 2315 54-55 0.9294745287046382 2315 56-57 1.0201859174757288 2315 58-59 0.9840117222592468 2315 60-61 0.9447604989468559 2315 62-63 1.0374475299776051 2315 64-65 1.1003152288650853 2315 66-67 0.9850885501512181 2315 68-69 1.1675477971834454 2315 70-71 1.179154188065958 2315 72-73 1.1610156260075044 2315 74-75 1.276298007995969 2315 76-77 1.2261781933532845 2315 78-79 1.1852303483241293 2315 80-81 1.2609427779666582 2315 82-83 1.4400757902945749 2315 84-85 1.4165698154417008 2315 86-87 1.4592316608534581 2315 88-89 1.6378561087060746 2315 90-91 1.8040352515294842 2315 92-93 1.9582574061947362 2315 94-95 2.007784073898339 2315 96-97 2.052507241301882 2315 98-99 2.253253039859864 2315 100 2.251491616982868 2316 1 0.06483457877096654 2316 2 0.03306949153087402 2316 3 0.11486328487400854 2316 4 0.16953627833731844 2316 5 0.12153965236539221 2316 6 0.2617644939779069 2316 7 0.25561744484544846 2316 8 0.2223166666281884 2316 9 0.42636711130712257 2316 10-11 0.23180805603759325 2316 12-13 0.36292231138941844 2316 14-15 0.5788042905685913 2316 16-17 0.5474082227542354 2316 18-19 0.4379388273080238 2316 20-21 0.42861626674456943 2316 22-23 0.31405814384292086 2316 24-25 0.46180454710080454 2316 26-27 0.5448738670299065 2316 28-29 0.7150983204606263 2316 30-31 0.7307237698483036 2316 32-33 0.7866985476899515 2316 34-35 0.8902664534292555 2316 36-37 0.8753075544881028 2316 38-39 1.0314204831013711 2316 40-41 0.929393134008933 2316 42-43 1.0438979315106351 2316 44-45 1.089846020768917 2316 46-47 1.1190712095890518 2316 48-49 1.2547659847179418 2316 50-51 1.089685746219665 2316 52-53 1.0188969060210908 2316 54-55 0.958497676196707 2316 56-57 1.1639480680422025 2316 58-59 1.1518282819061767 2316 60-61 1.1575315027204809 2316 62-63 1.1516761137953964 2316 64-65 1.178951616030183 2316 66-67 1.33551025064925 2316 68-69 1.2788191951212298 2316 70-71 1.1780363995942196 2316 72-73 1.1733830039799713 2316 74-75 1.3586586949362882 2316 76-77 1.4438924782189915 2316 78-79 1.4184674459181572 2316 80-81 1.574841286422302 2316 82-83 1.47372633597222 2316 84-85 1.6273597093949945 2316 86-87 1.7366764545346136 2316 88-89 1.8366639848613069 2316 90-91 1.878500562961598 2316 92-93 1.997809913189137 2316 94-95 2.2145752593863115 2316 96-97 2.2573073461860353 2316 98-99 2.4242064716338234 2316 100 2.359864062659071 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 9.0 1 86.0 2 53.0 3 37.0 4 40.0 5 25.0 6 40.0 7 275.0 8 929.0 9 1468.0 10 1846.0 11 2163.0 12 2512.0 13 2911.0 14 3354.0 15 4465.0 16 5916.0 17 8133.0 18 10227.0 19 12577.0 20 15382.0 21 18370.0 22 19756.0 23 20139.0 24 19951.0 25 20818.0 26 22866.0 27 26355.0 28 30695.0 29 36671.0 30 43881.0 31 53686.0 32 67706.0 33 86248.0 34 118088.0 35 159726.0 36 224627.0 37 318681.0 38 292231.0 39 76745.0 40 94.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.249049137778705 18.81012592225669 12.424387262356863 23.516437677607737 2 16.546241101245478 20.771962612493688 39.8372981134872 22.844498172773633 3 19.04893524624906 26.684098863919576 30.48947967666782 23.777486213163545 4 13.008726183117428 16.892516622884447 37.783141252451 32.31561594154712 5 12.863722709412162 37.48939602904057 34.424580973281174 15.222300288266094 6 29.527130244958 36.63136913063019 19.043200968055217 14.798299656356592 7 26.121752691761042 33.56150080320426 22.812967656060824 17.503778848973884 8 25.784333289951267 34.84430176565008 20.991656182667416 18.379708761731234 9 27.558354264853957 15.678253562214186 20.84391958363287 35.919472589298984 10-11 24.808733814380997 25.839934436241908 28.918874681608603 20.432457067768496 12-13 25.183454190239452 24.234778565240173 28.368223559635915 22.213543684884463 14-15 23.07151887810078 25.6256344234744 26.609256431836144 24.693590266588682 16-17 21.9164707957131 28.01093408038949 27.5232370891408 22.549358034756615 18-19 21.420007626677876 27.35196135431065 29.7576896312163 21.470341387795173 20-21 22.385625497464286 26.911368741274046 29.420713769638002 21.282291991623662 22-23 22.30211438434783 26.60131437046974 29.260962965747144 21.835608279435277 24-25 21.883443154880908 26.29998383829272 29.655027057126578 22.161545949699793 26-27 21.721072367146686 26.596306907707383 29.448782701965083 22.233838023180848 28-29 21.532898628407082 27.572828108836976 29.23222945573995 21.66204380701599 30-31 22.599404328591824 26.762281313946357 29.401258299474346 21.237056057987473 32-33 22.428965925213166 26.78967587708177 29.048211487869423 21.733146709835637 34-35 22.33122665111544 26.938537481239216 29.299324568324824 21.43091129932052 36-37 22.015365319550884 26.718464578289286 29.329822956499818 21.936347145660015 38-39 22.071047779688218 26.90059561618853 29.27204489687233 21.756311707250926 40-41 22.034053155906047 26.182263111528986 29.77654766768177 22.007136064883202 42-43 21.74628852968092 26.576426145752464 29.114813063912898 22.562472260653717 44-45 21.034410865134014 27.035137836914842 29.659667900552343 22.270783397398805 46-47 21.0212322212933 26.403535686389727 29.361575870901063 23.213656221415913 48-49 21.368187459732315 26.54674174432956 29.24908649469594 22.83598430124218 50-51 21.2812692817238 26.828102582963094 29.195804296900523 22.694823838412574 52-53 21.045797714350822 27.094159041136656 28.838022782460786 23.02202046205174 54-55 20.728599367222397 26.746348486894632 29.323235650230895 23.201816495652075 56-57 21.279734320339593 26.560649670517545 28.787660146807447 23.371955862335415 58-59 20.90818389248935 26.674243541581717 29.06677193317312 23.350800632755814 60-61 21.34009959730861 26.611054699878107 28.855434714727497 23.193410988085795 62-63 21.529080051603295 26.394008836861545 28.696149471447896 23.380761640087268 64-65 21.18872970667746 26.218669133910634 28.622930912350192 23.969670247061714 66-67 21.405004009001676 26.046395700754122 28.2153046422013 24.333295648042906 68-69 21.043783408350013 26.317842480587515 28.435446812374256 24.202927298688216 70-71 21.28292731591861 26.081600608411637 28.429074789231922 24.206397286437827 72-73 21.564022706307277 26.32612989326416 28.019039522045535 24.09080787838303 74-75 21.916743212303043 26.131220182258126 27.948683921021672 24.00335268441716 76-77 21.836949045817914 26.024601242406973 27.996019579459663 24.142430132315447 78-79 21.93715769899715 26.096924379232505 28.005252470118048 23.960665451652297 80-81 22.27289417002761 25.991413331020425 27.72933200341154 24.006360495540424 82-83 22.631401525686474 25.72625648254851 28.061419490925154 23.58092250083986 84-85 22.9098219311181 25.904126629330126 27.50526355044437 23.680787889107407 86-87 22.579919632274294 26.07533751553872 27.757942817495877 23.586800034691105 88-89 22.930079571793136 25.384104549177493 27.71861138070635 23.967204498323017 90-91 23.77564676525579 25.017903261385882 27.530199918065655 23.676250055292673 92-93 23.96579453999768 24.999110979532873 27.43112404278516 23.60397043768429 94-95 23.756166654571643 24.81606650778336 27.450044423910214 23.97772241373478 96-97 24.008617100509326 24.51984905328701 27.372651721569696 24.098882124633967 98-99 24.07179949805119 24.56663543943652 27.16821067971271 24.19335438279958 100 24.671346529397375 24.416959230372886 26.973265334800633 23.938428905429106 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1350.0 1 954.0 2 1123.0 3 1613.0 4 1092.0 5 728.5 6 1024.0 7 1636.5 8 2442.5 9 2812.0 10 2353.0 11 1854.5 12 1814.5 13 1941.5 14 2164.0 15 2349.0 16 2531.0 17 2757.0 18 2969.0 19 3193.5 20 3477.5 21 3820.5 22 4252.5 23 4863.5 24 5810.5 25 6876.0 26 8140.5 27 9854.5 28 11677.5 29 14055.0 30 16944.5 31 19699.5 32 23258.5 33 26908.5 34 30085.0 35 33262.5 36 36807.5 37 41208.0 38 44210.5 39 45341.0 40 46015.5 41 46965.0 42 48874.0 43 51893.5 44 55331.5 45 59731.5 46 64464.5 47 68646.0 48 72483.0 49 76024.0 50 78510.5 51 79171.0 52 77094.5 53 72829.5 54 67769.5 55 60734.5 56 53765.5 57 46621.0 58 38965.5 59 32094.5 60 25118.5 61 19134.0 62 14869.5 63 11526.5 64 8445.5 65 5941.0 66 4318.5 67 3294.0 68 2551.5 69 1922.0 70 1495.0 71 1151.5 72 840.5 73 652.5 74 502.0 75 381.5 76 288.5 77 191.5 78 131.5 79 101.5 80 82.5 81 60.0 82 48.5 83 38.5 84 26.0 85 19.5 86 17.0 87 14.0 88 8.5 89 7.0 90 8.0 91 8.5 92 7.5 93 4.0 94 2.5 95 2.5 96 2.0 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21488256901736752 2 0.1488048783025838 3 0.023991462507992337 4 0.02248838292917836 5 0.02757572919593336 6 0.05520926914489802 7 0.06249342402684269 8 0.05532489065096064 9 0.04225966046588529 10-11 0.04497676585835672 12-13 0.042779957243167056 14-15 0.020667344208692193 16-17 0.018904116241237334 18-19 0.01887521086472168 20-21 0.02234385604660009 22-23 0.025263299074681084 24-25 0.022286045293568785 26-27 0.019482223771550403 28-29 0.019250980759425176 30-31 0.017632279674548584 32-33 0.026217176499697646 34-35 0.02563906896938458 36-37 0.02503205606255586 38-39 0.02292196357691316 40-41 0.02297977432994447 42-43 0.029743632434607363 44-45 0.03997613572114868 46-47 0.0452369142469976 48-49 0.04208622820679138 50-51 0.02826945823230904 52-53 0.04489004972880976 54-55 0.0426932411136201 56-57 0.0452369142469976 58-59 0.04838760028720382 60-61 0.024164894767086255 62-63 0.025147677568618472 64-65 0.026419514135307225 66-67 0.0317091980376718 68-69 0.03532237010212848 70-71 0.03986051421508607 72-73 0.04318463251438621 74-75 0.02436723240269583 76-77 0.018297103334408614 78-79 0.017921333439705117 80-81 0.020638438832176538 82-83 0.019135359253362565 84-85 0.011475434476714407 86-87 0.013412094703263187 88-89 0.019511129148066058 90-91 0.020147047431410433 92-93 0.020262668937473045 94-95 0.024309421649664525 96-97 0.02485862380346194 98-99 0.030177213082342166 100 0.030581888353561317 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1729782.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.9315791900242 #Duplication Level Percentage of deduplicated Percentage of total 1 84.9158908786564 61.93050020108183 2 9.757187803240361 14.232142298879252 3 2.466054120271764 5.395596640784568 4 1.0371252125351298 3.02556718271906 5 0.5399498205481125 1.9689696547972009 6 0.3387159573850764 1.482185380137274 7 0.22271174999577634 1.1369903741956082 8 0.15534222222190064 0.9063482865224707 9 0.09687347107712822 0.6358621704546688 >10 0.44311927125704725 5.331789042820077 >50 0.016286144594321397 0.8076563465659242 >100 0.009744177071551023 1.2649813086931223 >500 3.330570484933689E-4 0.17895934891524784 >1k 4.995855727400534E-4 0.7751059143380467 >5k 1.6652852424668446E-4 0.9273458490956056 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8278 0.478557413593158 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6993 0.40427059594792863 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4051 0.234191360529824 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2235 0.12920703302497077 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2131 0.12319471470971487 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.7810753031306836E-5 0.0 10-11 0.0 0.0 0.0 5.7810753031306836E-5 0.0 12-13 0.0 0.0 0.0 8.671612954696025E-5 8.671612954696025E-5 14-15 0.0 0.0 0.0 1.734322590939205E-4 1.1562150606261367E-4 16-17 0.0 0.0 0.0 2.8905376515653416E-4 1.1562150606261367E-4 18-19 0.0 0.0 0.0 4.3358064773480127E-4 1.734322590939205E-4 20-21 0.0 0.0 0.0 9.249720485009093E-4 2.0233763560957392E-4 22-23 5.7810753031306836E-5 0.0 0.0 0.002659294639440114 2.3124301212522734E-4 24-25 5.7810753031306836E-5 0.0 0.0 0.007891167788773383 2.3124301212522734E-4 26-27 5.7810753031306836E-5 0.0 0.0 0.01826819795789296 2.3124301212522734E-4 28-29 5.7810753031306836E-5 0.0 0.0 0.05309917665925533 2.3124301212522734E-4 30-31 5.7810753031306836E-5 0.0 0.0 0.16114747407476782 2.3124301212522734E-4 32-33 5.7810753031306836E-5 0.0 0.0 0.38828592273477236 2.3124301212522734E-4 34-35 5.7810753031306836E-5 0.0 0.0 0.7282709613118878 3.46864518187841E-4 36-37 5.7810753031306836E-5 0.0 0.0 1.2231310072598744 3.46864518187841E-4 38-39 5.7810753031306836E-5 0.0 0.0 1.899459006973133 3.757698947034944E-4 40-41 5.7810753031306836E-5 0.0 0.0 2.6252441059046747 4.0467527121914785E-4 42-43 5.7810753031306836E-5 0.0 0.0 3.3064571142490786 4.0467527121914785E-4 44-45 5.7810753031306836E-5 0.0 0.0 4.100140942615891 4.3358064773480127E-4 46-47 5.7810753031306836E-5 0.0 0.0 4.988432068318435 4.624860242504547E-4 48-49 5.7810753031306836E-5 0.0 0.0 5.88224412093547 5.202967772817615E-4 50-51 5.7810753031306836E-5 0.0 0.0 6.762846416484852 5.202967772817615E-4 52-53 5.7810753031306836E-5 0.0 0.0 7.60410849459643 5.781075303130683E-4 54-55 5.7810753031306836E-5 0.0 0.0 8.525033790385146 5.781075303130683E-4 56-57 5.7810753031306836E-5 0.0 0.0 9.534120484546607 5.781075303130683E-4 58-59 5.7810753031306836E-5 0.0 0.0 10.608735667269055 5.781075303130683E-4 60-61 5.7810753031306836E-5 0.0 0.0 11.647016791711327 5.781075303130683E-4 62-63 5.7810753031306836E-5 0.0 0.0 12.681193352688373 6.359182833443752E-4 64-65 5.7810753031306836E-5 0.0 0.0 13.685915334995972 6.359182833443752E-4 66-67 5.7810753031306836E-5 0.0 0.0 14.78625630281735 6.359182833443752E-4 68-69 5.7810753031306836E-5 0.0 0.0 15.96565347540904 6.359182833443752E-4 70-71 5.7810753031306836E-5 0.0 0.0 17.131320594155795 6.359182833443752E-4 72-73 5.7810753031306836E-5 0.0 0.0 18.213508985525344 6.359182833443752E-4 74-75 5.7810753031306836E-5 0.0 0.0 19.273729290742995 6.359182833443752E-4 76-77 5.7810753031306836E-5 0.0 0.0 20.404478714658843 6.93729036375682E-4 78-79 5.7810753031306836E-5 0.0 0.0 21.56132969356832 6.93729036375682E-4 80-81 5.7810753031306836E-5 0.0 0.0 22.692512698131903 7.226344128913355E-4 82-83 5.7810753031306836E-5 5.7810753031306836E-5 0.0 23.79692932404199 7.515397894069888E-4 84-85 5.7810753031306836E-5 5.7810753031306836E-5 0.0 24.86674621426284 7.515397894069888E-4 86-87 5.7810753031306836E-5 5.7810753031306836E-5 0.0 25.93219839262982 7.804451659226422E-4 88 5.7810753031306836E-5 5.7810753031306836E-5 5.7810753031306836E-5 26.753544666322114 8.093505424382957E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3175 0.0 57.61211 1 GTATCAA 6035 0.0 42.183468 1 TACCTGG 1500 0.0 39.86431 2 GTGGTAT 1150 0.0 36.895252 1 ATCAACG 7270 0.0 34.401917 3 TATCAAC 7445 0.0 33.834587 2 CAACGCA 7405 0.0 33.64777 5 ACCTGGG 1725 0.0 33.521347 3 ATAACGC 215 0.0 32.79882 3 TCAACGC 7680 0.0 32.625626 4 AACGCAG 7880 0.0 31.799404 6 TGGTATC 1365 0.0 31.389223 2 TAACGCA 240 0.0 29.381428 4 GTACATG 5585 0.0 29.122034 1 ACGCAGA 8640 0.0 28.730167 7 TACATGG 5720 0.0 27.986895 2 CGCAGAG 8880 0.0 27.741907 8 ACATGGG 5770 0.0 26.479694 3 GTATAAC 435 0.0 26.010447 1 GCAGAGT 9480 0.0 25.88541 9 >>END_MODULE