FastQCFastQC Report
Mon 6 Jun 2016
ERR1211456_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211456_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729782
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73850.42693241113620095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65210.37698392051715185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39820.2302024185706638No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23210.13417875778566316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32350.060.029441
TACCTGG16150.042.769282
GTATCAA60450.041.3812751
ACCTGGG18850.036.3949553
ATCAACG73150.033.8529053
CAACGCA73850.033.593715
TATCAAC74650.033.2976042
TCAACGC76450.032.5769654
AACGCAG80200.031.2844776
CCTGGGG16550.031.2325124
GTGGTAT11200.031.0672071
ACGCAGA88650.028.0904797
CGCAGAG89600.027.7926458
CTGGGGG11950.027.1303715
GTACATG58650.027.0178281
TACATGG59200.026.3513872
GGGTTAG4500.025.0776751
GTACCTG28800.024.9797151
GCAGAGT97550.024.9014879
ACATGGG59350.024.702083
Produced by FastQC (version 0.11.5)