FastQCFastQC Report
Mon 6 Jun 2016
ERR1211455_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211455_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3995908
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163150.4082926834151337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140670.3520351319399746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76130.19051990185960238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71000.055.13171
GTATCAA128350.040.3331
TACCTGG29350.033.3532452
ATCAACG154850.032.816433
TATCAAC156400.032.6795432
CAACGCA155150.032.601075
TCAACGC163150.031.0893174
AACGCAG165900.030.8856646
GTGGTAT27900.030.7274061
ACGCAGA186400.026.9851957
TAACGCC7600.026.5969414
CGCAGAG189100.026.4997948
ACCTGGG36750.026.094543
GTACATG129950.025.6634881
CTAACGC7700.025.6410243
TCTAACG7800.025.3418052
GTACTAG15900.025.1814371
TACATGG131200.024.8230482
GCAGAGT201400.024.1329679
ACATGGG133200.023.7160243
Produced by FastQC (version 0.11.5)