##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211455_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3995908 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.37220226291496 31.0 31.0 34.0 28.0 34.0 2 31.41756291686395 31.0 31.0 34.0 28.0 34.0 3 31.482583182595796 31.0 31.0 34.0 28.0 34.0 4 35.11781602579439 37.0 35.0 37.0 32.0 37.0 5 35.16931245664315 37.0 35.0 37.0 32.0 37.0 6 35.23711707076339 37.0 35.0 37.0 32.0 37.0 7 35.162839584895345 37.0 35.0 37.0 32.0 37.0 8 35.174603118990724 37.0 35.0 37.0 32.0 37.0 9 36.74425061838261 39.0 37.0 39.0 32.0 39.0 10-11 36.67532010246482 39.0 37.0 39.0 32.0 39.0 12-13 36.58763540101524 39.0 37.0 39.0 32.0 39.0 14-15 37.77169444341561 40.0 37.0 41.0 32.0 41.0 16-17 37.76900781999986 40.0 37.0 41.0 32.0 41.0 18-19 37.76988471706556 40.0 37.0 41.0 32.0 41.0 20-21 37.75106121061846 40.0 37.0 41.0 32.0 41.0 22-23 37.62295565863879 40.0 37.0 41.0 32.0 41.0 24-25 37.527144518842775 40.0 37.0 41.0 32.0 41.0 26-27 37.38063926897216 40.0 36.5 41.0 31.0 41.0 28-29 37.25126967387638 40.0 36.0 41.0 31.0 41.0 30-31 37.08967423674419 39.0 36.0 41.0 31.0 41.0 32-33 36.88549523662707 39.0 35.5 41.0 30.0 41.0 34-35 36.75062538977374 39.0 35.0 41.0 30.0 41.0 36-37 36.60577908700601 39.0 35.0 41.0 30.0 41.0 38-39 36.439727591325926 39.0 35.0 40.5 30.0 41.0 40-41 36.25910531473697 39.0 35.0 40.0 29.0 41.0 42-43 36.04494397768917 38.5 35.0 40.0 28.0 41.0 44-45 35.8888439123223 38.0 35.0 40.0 27.5 41.0 46-47 35.63937695762765 38.0 34.5 40.0 27.0 41.0 48-49 35.372291604311215 38.0 34.0 40.0 26.0 41.0 50-51 34.688723564206185 37.5 33.5 39.5 25.0 40.5 52-53 34.98211995871777 38.0 33.5 39.5 26.0 40.5 54-55 35.37613428537394 38.0 34.0 40.0 26.0 41.0 56-57 35.28089172723696 38.0 34.0 40.0 26.0 41.0 58-59 35.058444413635144 38.0 34.0 40.0 26.0 41.0 60-61 34.820573071251886 37.5 34.0 40.0 26.0 41.0 62-63 34.53826939959579 37.0 33.0 40.0 25.5 41.0 64-65 34.32289432089027 36.5 33.0 40.0 25.0 41.0 66-67 34.117473425314095 36.0 33.0 39.0 25.0 41.0 68-69 33.769432003940025 35.5 33.0 39.0 24.0 41.0 70-71 33.39566226249453 35.0 32.5 39.0 23.5 41.0 72-73 33.016426804621126 35.0 32.0 38.0 23.0 40.0 74-75 32.63359767041683 35.0 32.0 37.0 22.0 39.5 76-77 32.25102980348897 35.0 31.0 37.0 20.5 39.0 78-79 31.85095602801666 35.0 31.0 36.0 20.0 39.0 80-81 31.448715160609304 35.0 31.0 36.0 19.5 37.5 82-83 31.07735550968641 34.5 30.0 35.0 18.5 37.0 84-85 30.74749368604082 34.0 30.0 35.0 18.0 37.0 86-87 30.412737105058476 34.0 29.0 35.0 16.5 36.0 88-89 30.08915520577551 34.0 29.0 35.0 15.0 36.0 90-91 29.77165865680591 34.0 29.0 35.0 11.5 36.0 92-93 29.464705268489666 34.0 28.5 35.0 10.0 35.0 94-95 29.170237527991134 34.0 27.0 35.0 10.0 35.0 96-97 28.872267329478056 34.0 26.5 35.0 10.0 35.0 98-99 28.544693221165254 33.5 25.5 35.0 9.0 35.0 100 27.27954347297285 32.0 24.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4110203120900877 1101 2 -0.37632845166356077 1101 3 -0.48619249775732953 1101 4 -0.3632333708724502 1101 5 -0.29055475861779456 1101 6 -0.39089421679587844 1101 7 -0.3200058778978274 1101 8 -0.35422484762131035 1101 9 -0.5331702679005303 1101 10-11 -0.4511708765040794 1101 12-13 -0.4958812149307974 1101 14-15 -0.7406451332849926 1101 16-17 -0.7108647506135171 1101 18-19 -0.6299723953837315 1101 20-21 -0.8004025990944044 1101 22-23 -0.852662023336201 1101 24-25 -0.8062822004209664 1101 26-27 -0.8963792513323128 1101 28-29 -0.9424867567393775 1101 30-31 -0.8668651669512144 1101 32-33 -0.9643679946394244 1101 34-35 -0.8697039402895328 1101 36-37 -0.9485241657931738 1101 38-39 -0.8943799333923081 1101 40-41 -1.1240968314226976 1101 42-43 -1.229155794259377 1101 44-45 -1.159647873333519 1101 46-47 -1.2456868784387538 1101 48-49 -1.2325749810607078 1101 50-51 -1.1528273664470134 1101 52-53 -0.9680920138799465 1101 54-55 -0.803594019249168 1101 56-57 -0.8530070263323921 1101 58-59 -0.8409483707425736 1101 60-61 -0.8315568424911888 1101 62-63 -0.8600431405368099 1101 64-65 -0.8613227714202054 1101 66-67 -0.8630538743015208 1101 68-69 -0.9574628835412327 1101 70-71 -1.0012213018921585 1101 72-73 -1.197012412263291 1101 74-75 -1.2706682659242112 1101 76-77 -1.0986594462156418 1101 78-79 -1.1845699225925443 1101 80-81 -1.3841583992063633 1101 82-83 -1.4110799325052845 1101 84-85 -1.5645067678882612 1101 86-87 -1.5560609627426594 1101 88-89 -1.6925212037928041 1101 90-91 -1.8202036544831657 1101 92-93 -1.9150018868997556 1101 94-95 -2.032234510214362 1101 96-97 -2.123142882669029 1101 98-99 -2.15351050136584 1101 100 -2.1610900331225658 1102 1 -0.329877052818663 1102 2 -0.3185545884921126 1102 3 -0.4309069880878944 1102 4 -0.41794158494046485 1102 5 -0.28004296067577883 1102 6 -0.3190591144807442 1102 7 -0.4607539661994906 1102 8 -0.3322129475864273 1102 9 -0.5673859196787205 1102 10-11 -0.4185748847227444 1102 12-13 -0.6062833854778731 1102 14-15 -0.7823725596481594 1102 16-17 -0.717520527374468 1102 18-19 -0.7798096118876643 1102 20-21 -0.8008383962437975 1102 22-23 -0.7159355651599952 1102 24-25 -0.7550707988705057 1102 26-27 -0.8719324929503429 1102 28-29 -0.8708861666350813 1102 30-31 -0.8166829066980412 1102 32-33 -0.7908972967988959 1102 34-35 -0.9538832756385958 1102 36-37 -0.9616145432203282 1102 38-39 -1.1940017377160785 1102 40-41 -1.319718252992395 1102 42-43 -1.2040428705080544 1102 44-45 -1.169483183601649 1102 46-47 -1.2069967713505036 1102 48-49 -1.2550534191227314 1102 50-51 -1.0245583039006192 1102 52-53 -1.0336401922945768 1102 54-55 -1.0654128695855505 1102 56-57 -0.9781655339833293 1102 58-59 -0.910821406396721 1102 60-61 -1.0123940868728738 1102 62-63 -0.9487893045198135 1102 64-65 -1.186953166613776 1102 66-67 -1.1822021636244173 1102 68-69 -1.2520523383653241 1102 70-71 -1.3069543249033622 1102 72-73 -1.174495548104069 1102 74-75 -1.1967083134520209 1102 76-77 -1.225416780891127 1102 78-79 -1.2966835613706564 1102 80-81 -1.4853900686394574 1102 82-83 -1.472753366434624 1102 84-85 -1.6622870956854712 1102 86-87 -1.7282443863270949 1102 88-89 -1.7785572730893726 1102 90-91 -1.712974010331429 1102 92-93 -1.8685848567548575 1102 94-95 -1.919344551254941 1102 96-97 -2.0162221542006407 1102 98-99 -2.094307526432466 1102 100 -2.1224152964731857 1103 1 -0.44544118769575647 1103 2 -0.5113259313468319 1103 3 -0.5107577535021015 1103 4 -0.3226129011294958 1103 5 -0.35571172146741503 1103 6 -0.33858011157550294 1103 7 -0.29956032274078837 1103 8 -0.4208202443460394 1103 9 -0.6375079832456834 1103 10-11 -0.5773032732675389 1103 12-13 -0.6745590636455105 1103 14-15 -0.6889416490575186 1103 16-17 -0.8131766627770958 1103 18-19 -0.8102640092780078 1103 20-21 -0.8397690631115537 1103 22-23 -0.8347681520353447 1103 24-25 -0.8699844230179679 1103 26-27 -0.9429893328558947 1103 28-29 -1.0075101733769145 1103 30-31 -0.8788621312472671 1103 32-33 -0.8920727640510151 1103 34-35 -0.8756359890477157 1103 36-37 -0.904594935773531 1103 38-39 -0.928171956569102 1103 40-41 -0.890085555411666 1103 42-43 -0.8827687401944502 1103 44-45 -0.9201777354819711 1103 46-47 -1.0383596680998508 1103 48-49 -1.0246800294623455 1103 50-51 -0.9604380812770756 1103 52-53 -0.859103168574542 1103 54-55 -0.8664335514343051 1103 56-57 -0.8420058834516198 1103 58-59 -0.7182994616203615 1103 60-61 -0.7749107140592884 1103 62-63 -0.8641128650971908 1103 64-65 -0.7763459351578987 1103 66-67 -0.8328127535581515 1103 68-69 -0.7938128338030026 1103 70-71 -0.8502560662585879 1103 72-73 -0.896194813514775 1103 74-75 -1.0471547111346666 1103 76-77 -1.0942505339046207 1103 78-79 -1.1661668922032717 1103 80-81 -1.140418028745092 1103 82-83 -1.1001698184670872 1103 84-85 -1.3355389185537518 1103 86-87 -1.4280989300288986 1103 88-89 -1.5303564687650208 1103 90-91 -1.5563817536442102 1103 92-93 -1.607710618534604 1103 94-95 -1.5655046915646835 1103 96-97 -1.8063447697396633 1103 98-99 -1.7435634525521735 1103 100 -1.940669556570196 1104 1 -0.4612940867786044 1104 2 -0.4083222164572433 1104 3 -0.43654345184278753 1104 4 -0.3118255087462174 1104 5 -0.34650941122885115 1104 6 -0.3819242093702826 1104 7 -0.29397317558007785 1104 8 -0.42848464246536366 1104 9 -0.4127066456176465 1104 10-11 -0.41161197961535123 1104 12-13 -0.5805488181312981 1104 14-15 -0.7491782332828336 1104 16-17 -0.8311827619460317 1104 18-19 -0.7524406758746451 1104 20-21 -0.751837748824606 1104 22-23 -0.7268948437983127 1104 24-25 -0.6667564081165409 1104 26-27 -0.6568363362242096 1104 28-29 -0.8761096414560043 1104 30-31 -0.9208751955744887 1104 32-33 -0.8483657725529312 1104 34-35 -0.8397913866567848 1104 36-37 -0.9511630240124092 1104 38-39 -1.025023830608049 1104 40-41 -0.936908230973998 1104 42-43 -0.9614430331692532 1104 44-45 -1.009991772465888 1104 46-47 -1.0164177574091013 1104 48-49 -1.1352901610451624 1104 50-51 -0.9408205447697497 1104 52-53 -0.882966584495442 1104 54-55 -0.831507918095916 1104 56-57 -0.7202909044445818 1104 58-59 -0.7492879368211902 1104 60-61 -0.7818772574298194 1104 62-63 -0.9468451653530963 1104 64-65 -0.9385625754655251 1104 66-67 -0.794412312976263 1104 68-69 -0.920423261179593 1104 70-71 -0.9550674781113102 1104 72-73 -0.9042252217909734 1104 74-75 -0.9252510135510121 1104 76-77 -0.9795363023077996 1104 78-79 -0.9167838153463705 1104 80-81 -1.1022630569743903 1104 82-83 -1.274056038363586 1104 84-85 -1.2229760787304613 1104 86-87 -1.4040718425840595 1104 88-89 -1.4268588536984375 1104 90-91 -1.4653113241792113 1104 92-93 -1.5266989469158503 1104 94-95 -1.6420553330500987 1104 96-97 -1.7572972156593814 1104 98-99 -1.7642036475236615 1104 100 -1.6424127160123447 1105 1 -0.4753205397711824 1105 2 -0.4322383471859439 1105 3 -0.41042006514572904 1105 4 -0.30280417098771295 1105 5 -0.3826038450029543 1105 6 -0.32717510355102775 1105 7 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-0.5403876042936915 1105 70-71 -0.5667473489052028 1105 72-73 -0.5513216987312077 1105 74-75 -0.5296434867068243 1105 76-77 -0.6323592967430827 1105 78-79 -0.7088115115483831 1105 80-81 -0.7354733004135916 1105 82-83 -0.8995453522196222 1105 84-85 -0.8814272943951735 1105 86-87 -0.8466745175185686 1105 88-89 -0.7818122611184073 1105 90-91 -0.8914118238725379 1105 92-93 -1.045848628295584 1105 94-95 -1.0654330749773244 1105 96-97 -1.233939298126007 1105 98-99 -1.0909793551122746 1105 100 -1.1527207627720912 1106 1 -0.4533091793951165 1106 2 -0.4550754146593192 1106 3 -0.4796263030174899 1106 4 -0.36434458313516416 1106 5 -0.4524020605878718 1106 6 -0.3765996026241325 1106 7 -0.4891838333385792 1106 8 -0.4487449847596068 1106 9 -0.5839732624829495 1106 10-11 -0.6750057755405834 1106 12-13 -0.5214133724458279 1106 14-15 -0.8083687542010765 1106 16-17 -0.7791884043201875 1106 18-19 -0.7859397689691505 1106 20-21 -0.7165134958951072 1106 22-23 -0.7384883471906107 1106 24-25 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54-55 1.187420651168999 2311 56-57 1.245360369148898 2311 58-59 1.1932271318978138 2311 60-61 1.1971406924641457 2311 62-63 1.2239115652186499 2311 64-65 1.2442369841112892 2311 66-67 1.2584455376953727 2311 68-69 1.2619195832562013 2311 70-71 1.2393756596508894 2311 72-73 1.271820334853885 2311 74-75 1.268768818559323 2311 76-77 1.3153743366633819 2311 78-79 1.265341139825523 2311 80-81 1.297365839329327 2311 82-83 1.3822620613118026 2311 84-85 1.3993817652295775 2311 86-87 1.4368146783590277 2311 88-89 1.4625252974576135 2311 90-91 1.5020441897288208 2311 92-93 1.5581111452325658 2311 94-95 1.6278412501777808 2311 96-97 1.6766847428601075 2311 98-99 1.8103595018751406 2311 100 1.7930729367948182 2312 1 0.4584253440214958 2312 2 0.5676280185796969 2312 3 0.5931884700029784 2312 4 0.4963181038672104 2312 5 0.544707054485734 2312 6 0.4083444357753194 2312 7 0.5856196699397955 2312 8 0.5856099070894132 2312 9 0.7743279378246726 2312 10-11 0.8148277312238079 2312 12-13 0.835012794841461 2312 14-15 1.086662105276389 2312 16-17 0.9969343696743564 2312 18-19 0.9959238601109703 2312 20-21 1.0324544863712575 2312 22-23 1.1198892602123962 2312 24-25 1.050923583263284 2312 26-27 1.08806176669043 2312 28-29 1.1197054986755575 2312 30-31 1.0227000106894266 2312 32-33 1.235779401485786 2312 34-35 1.2467397274699579 2312 36-37 1.2581213942505798 2312 38-39 1.375824178084386 2312 40-41 1.358282570833218 2312 42-43 1.389608958691035 2312 44-45 1.4013070044671707 2312 46-47 1.3511638157993815 2312 48-49 1.3766153501406393 2312 50-51 1.242551209063521 2312 52-53 1.123163994805111 2312 54-55 1.0539601823100568 2312 56-57 1.0576590211838592 2312 58-59 1.1145341686269532 2312 60-61 1.1176472365080983 2312 62-63 1.1024356857377953 2312 64-65 1.1336273122018312 2312 66-67 1.097599307612846 2312 68-69 1.1755373532464901 2312 70-71 1.1531662706769268 2312 72-73 1.1870060962446516 2312 74-75 1.2224018888355985 2312 76-77 1.2635085590119957 2312 78-79 1.2690126883613857 2312 80-81 1.1451637739486067 2312 82-83 1.3020833245485086 2312 84-85 1.40381656343326 2312 86-87 1.5153177347476507 2312 88-89 1.4280958693069614 2312 90-91 1.4517016143379458 2312 92-93 1.4903501685033227 2312 94-95 1.6018632239295343 2312 96-97 1.6755460445695043 2312 98-99 1.5213238740530635 2312 100 1.5649699038479383 2313 1 0.6436269633037384 2313 2 0.6776337146599793 2313 3 0.6974404079475249 2313 4 0.5558195556730396 2313 5 0.5585025486218171 2313 6 0.6016226128862172 2313 7 0.6516806668479518 2313 8 0.6931369791562574 2313 9 0.8429726068358008 2313 10-11 0.7115871500353919 2313 12-13 0.8412438181774675 2313 14-15 1.0643960109365693 2313 16-17 1.0676395094568818 2313 18-19 1.0320878973636027 2313 20-21 1.0630483531582726 2313 22-23 1.0985323203385278 2313 24-25 1.1199018382191213 2313 26-27 1.184717997265615 2313 28-29 1.1663444751916572 2313 30-31 1.1726713286171915 2313 32-33 1.2146201662394631 2313 34-35 1.2408083197493767 2313 36-37 1.3374490371802992 2313 38-39 1.378508237440144 2313 40-41 1.3051785207901716 2313 42-43 1.3954892092468683 2313 44-45 1.350224771621626 2313 46-47 1.37209072626068 2313 48-49 1.3898683721778866 2313 50-51 1.363631053230236 2313 52-53 1.1603541555994994 2313 54-55 1.1591242773749926 2313 56-57 1.1193327142112963 2313 58-59 1.1411840488120788 2313 60-61 1.0510134496544339 2313 62-63 1.0478179841487574 2313 64-65 1.118794781891829 2313 66-67 1.087921012905447 2313 68-69 1.0727763900845773 2313 70-71 1.0389224174914844 2313 72-73 1.088427853120173 2313 74-75 1.0498262970101635 2313 76-77 1.0741891352886057 2313 78-79 1.1181873841409526 2313 80-81 1.2444887817129278 2313 82-83 1.2686763963043326 2313 84-85 1.3781573127255058 2313 86-87 1.4354970066622101 2313 88-89 1.4974971400123955 2313 90-91 1.6222511762865182 2313 92-93 1.6787385004570794 2313 94-95 1.8016823252036893 2313 96-97 1.908974887709494 2313 98-99 1.9268525451454366 2313 100 2.0534717540656757 2314 1 0.548884374917705 2314 2 0.5692998963282676 2314 3 0.6272836054185902 2314 4 0.5162297286489874 2314 5 0.48778571518134584 2314 6 0.5671805805490067 2314 7 0.6430595690245866 2314 8 0.5632741775401371 2314 9 0.780786727158862 2314 10-11 0.7150466252481849 2314 12-13 0.4847039979142167 2314 14-15 0.9022716348888125 2314 16-17 0.9038499625795637 2314 18-19 0.9489879612958703 2314 20-21 1.000879758386013 2314 22-23 0.8743245706624592 2314 24-25 0.865909244034988 2314 26-27 0.9273644737049764 2314 28-29 0.9851937731400966 2314 30-31 0.9291617446845422 2314 32-33 0.9938723074890774 2314 34-35 1.0702021096988332 2314 36-37 1.1351092955961803 2314 38-39 1.2116881654211653 2314 40-41 1.196649361155984 2314 42-43 1.2329067175862818 2314 44-45 1.2740472210807852 2314 46-47 1.2343164487368767 2314 48-49 1.2797741410038839 2314 50-51 1.2390150297297708 2314 52-53 1.14749138592763 2314 54-55 1.1522645113382097 2314 56-57 1.050971419445034 2314 58-59 1.1198513914133343 2314 60-61 1.1086993466162767 2314 62-63 1.1313626236184717 2314 64-65 1.0782292110589466 2314 66-67 1.1167249937721593 2314 68-69 1.1419982324278664 2314 70-71 1.1553486999985978 2314 72-73 1.1354563810895542 2314 74-75 1.1383039607561969 2314 76-77 1.2079921950230528 2314 78-79 1.306138011451722 2314 80-81 1.3579233754148383 2314 82-83 1.2246782840784753 2314 84-85 1.3171562585651202 2314 86-87 1.434909888773472 2314 88-89 1.6146879841765056 2314 90-91 1.6754303953676484 2314 92-93 1.7219647651104104 2314 94-95 1.753450942905797 2314 96-97 1.9962514256174764 2314 98-99 1.9892449466138196 2314 100 1.9958040609528105 2315 1 0.48868460836138183 2315 2 0.4268313038575684 2315 3 0.494841865348981 2315 4 0.3639852038386877 2315 5 0.3362857864050568 2315 6 -0.3131290618654532 2315 7 0.18685696202425106 2315 8 0.3212278672471456 2315 9 0.5269113707745632 2315 10-11 0.5796181880664264 2315 12-13 0.6234622603959039 2315 14-15 0.7729199194604846 2315 16-17 0.6919417837591553 2315 18-19 0.7202083954841001 2315 20-21 0.7367508668607883 2315 22-23 0.6539904357966435 2315 24-25 0.8351976235149081 2315 26-27 0.8096679122155237 2315 28-29 0.8614483202955086 2315 30-31 0.9333286292744916 2315 32-33 0.9206730022922969 2315 34-35 0.9966755156165661 2315 36-37 1.0627229824542468 2315 38-39 1.063859042790945 2315 40-41 1.039731115342768 2315 42-43 1.2188349325925856 2315 44-45 1.116388907785769 2315 46-47 1.222877177047124 2315 48-49 1.2727277350372503 2315 50-51 1.1453074849066667 2315 52-53 1.105913281158621 2315 54-55 0.9347216229723401 2315 56-57 0.8876457825243591 2315 58-59 0.9303954980235289 2315 60-61 0.8790062676472559 2315 62-63 1.028638454969233 2315 64-65 1.1423465154435632 2315 66-67 1.0689185139029505 2315 68-69 1.1606715579797395 2315 70-71 1.1920699006389768 2315 72-73 1.1590662537585743 2315 74-75 1.1972957944796647 2315 76-77 1.1227107338102087 2315 78-79 1.1925497185032867 2315 80-81 1.1524929397170887 2315 82-83 1.3816789793069084 2315 84-85 1.36756406213226 2315 86-87 1.4478511090163124 2315 88-89 1.6169481994634545 2315 90-91 1.5485476125829507 2315 92-93 1.6428045245231004 2315 94-95 1.7780241215454886 2315 96-97 1.94728984602445 2315 98-99 2.129926738219627 2315 100 2.0412447018386217 2316 1 0.026128352735820926 2316 2 0.020847930009981752 2316 3 0.12060680919030631 2316 4 0.12846969310394485 2316 5 0.0888532064123666 2316 6 0.24170446230328935 2316 7 0.13680616102578114 2316 8 0.20472797235969864 2316 9 0.33519624007782056 2316 10-11 0.1295637336413975 2316 12-13 0.32464219471587086 2316 14-15 0.5251830029116817 2316 16-17 0.5237008780359318 2316 18-19 0.45660195320721897 2316 20-21 0.4430342298909622 2316 22-23 0.30547305344616404 2316 24-25 0.39182944996285585 2316 26-27 0.5365008812267149 2316 28-29 0.536436125198442 2316 30-31 0.5897083284689089 2316 32-33 0.6743019978449141 2316 34-35 0.6984627147446432 2316 36-37 0.7741934560322079 2316 38-39 0.7690310293773592 2316 40-41 0.7252632603940086 2316 42-43 0.7701897619530698 2316 44-45 0.6378871188313582 2316 46-47 0.6162390642562556 2316 48-49 0.8262774369462136 2316 50-51 0.5351245278354497 2316 52-53 0.3309036985677096 2316 54-55 0.2777618861823328 2316 56-57 0.32875713855791844 2316 58-59 0.4886668557715481 2316 60-61 0.54602825556033 2316 62-63 0.5925831963821437 2316 64-65 0.5139081818142799 2316 66-67 0.5054892526094719 2316 68-69 0.5291951042119152 2316 70-71 0.47646625864211245 2316 72-73 0.5185468548799079 2316 74-75 0.6077281642472556 2316 76-77 0.7529940682661049 2316 78-79 0.8050851761306888 2316 80-81 1.0151924749767396 2316 82-83 1.0160191888380687 2316 84-85 1.158530424074577 2316 86-87 1.2559408771768013 2316 88-89 1.324885503397926 2316 90-91 1.3416775582305789 2316 92-93 1.4731682955940855 2316 94-95 1.5358161660560583 2316 96-97 1.6928296449023001 2316 98-99 1.854727871394168 2316 100 1.6452437956255501 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 9.0 1 217.0 2 115.0 3 74.0 4 68.0 5 58.0 6 71.0 7 318.0 8 1193.0 9 2089.0 10 2925.0 11 3891.0 12 5142.0 13 6482.0 14 7644.0 15 9840.0 16 13018.0 17 17131.0 18 22278.0 19 27890.0 20 33559.0 21 39949.0 22 43663.0 23 44821.0 24 44829.0 25 47673.0 26 52898.0 27 60958.0 28 72076.0 29 85207.0 30 102408.0 31 126122.0 32 158873.0 33 202102.0 34 273565.0 35 375454.0 36 534013.0 37 737748.0 38 662625.0 39 176721.0 40 191.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.96857546365581 19.09125542897458 12.32277221117429 23.617396896195324 2 16.390300847943998 20.74076932986009 39.265014954429965 23.603914867765948 3 19.175887648577646 26.180279426728394 30.062991790916815 24.58084113377714 4 12.913299656346549 16.75488915805781 38.23567032197392 32.09614086362172 5 13.068623867908943 37.54146619872735 35.04931386145058 14.340596071913128 6 30.043007112599213 37.17869234941126 18.259544018440778 14.518756519548756 7 26.342104828236923 32.98918783833392 22.87982419281897 17.78888314061019 8 25.690376988101438 34.656188654300706 20.808361043227436 18.845073314370417 9 26.524805933335106 15.454155803122793 20.90331942499285 37.11771883854925 10-11 24.805611254555682 25.79075541530729 28.65576899629386 20.747864333843157 12-13 25.380984403110666 24.06234149121063 28.419170719567937 22.13750338611076 14-15 22.82677515879652 25.407493150720935 26.286763992697608 25.478967697784938 16-17 21.887888962255037 28.191656850676637 27.14946402942344 22.77099015764488 18-19 21.510074215782062 27.513541648806576 29.42020429896116 21.556179836450198 20-21 22.35344797808096 26.840833167712375 29.250242674338793 21.555476179867867 22-23 22.534547400640708 26.599183067806177 28.94514719347896 21.921122338074156 24-25 22.18099093672377 26.630102512378667 29.263292323292273 21.92561422760529 26-27 22.075528275921453 26.820037014732307 28.98730615949375 22.117128549852495 28-29 21.792724182195432 27.236065036649666 29.02705463452521 21.944156146629695 30-31 22.26658590429659 26.933741483658135 29.02489449998248 21.774778112062794 32-33 22.243112607954114 27.119660399258912 28.629976045867178 22.007250946919797 34-35 22.356861909719413 26.91720020095617 28.98683847234262 21.739099416981798 36-37 22.25334202763235 26.619048274636388 28.962053389837934 22.16555630789333 38-39 22.165957763692195 26.66443055076661 29.146356497366412 22.023255188174783 40-41 21.972616270461547 26.320215441267486 29.527190924272524 22.179977363998443 42-43 21.743173810462547 26.55191533835245 29.070361539279926 22.634549311905076 44-45 21.517235975123857 26.905122712111535 29.21458540553166 22.363055907232944 46-47 21.552242677309916 26.619812232585033 28.98961942654289 22.838325663562163 48-49 21.710851614134008 26.572593657626385 28.95622246551281 22.760332262726795 50-51 21.517758701246066 26.748725763918973 28.799631625210942 22.93388390962402 52-53 21.192427529383192 27.066237211159667 28.656516127740062 23.084819131717076 54-55 21.06302149887238 26.861535665215246 28.823290244791522 23.25215259112085 56-57 21.4836242440979 26.649489885398687 28.488603899977257 23.378281970526153 58-59 21.265892572276694 26.600586598518518 28.64752224155796 23.48599858764683 60-61 21.33495621047757 26.627171093595997 28.46425967703076 23.573613018895674 62-63 21.458362902202328 26.385005823659153 28.386827641466855 23.769803632671664 64-65 21.261201049092183 26.331575459353314 28.368894454342524 24.038329037211984 66-67 21.59050727823983 26.231978694671902 28.072325666013676 24.105188361074596 68-69 21.406878340588474 26.318001053692374 28.08154011822934 24.193580487489815 70-71 21.59247166717092 26.094405085382466 28.005278955865293 24.307844291581322 72-73 21.91231583604122 26.052031959349048 27.91977815827404 24.115874046335698 74-75 22.049836637607083 26.09718138075484 27.8501809470826 24.00280103455548 76-77 22.134927179481277 26.05807294479287 27.712095478586242 24.094904397139615 78-79 22.280434703438186 26.155230882230924 27.617274552062653 23.94705986226824 80-81 22.428344578798185 26.10735872916068 27.550078946867806 23.914217745173328 82-83 22.69208545343935 25.667256919366494 27.941846947378984 23.69881067981517 84-85 22.87496700944309 25.750479637959216 27.562310386422194 23.812242966175496 86-87 22.73453334608132 25.791634984414586 27.74884729997132 23.724984369532777 88-89 23.288326476301755 25.30119501828333 27.52397958634541 23.886498919069506 90-91 23.77136263429573 25.1749403495115 27.328365223061223 23.725331793131545 92-93 24.0056964133096 25.018750881538608 27.30023224435756 23.67532046079423 94-95 23.7379957894916 24.805241145535632 27.459956553240428 23.99680651173234 96-97 24.01311598351228 24.609515508615452 27.28641720482216 24.090951303050108 98-99 24.172320541470274 24.68646997699232 27.013297192592383 24.127912288945026 100 24.886763990974163 24.401928128740842 26.740169732604315 23.971138147680684 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2327.0 1 1587.0 2 1755.0 3 2663.5 4 1960.5 5 1327.5 6 1882.0 7 3092.5 8 4591.0 9 5373.0 10 4541.0 11 3613.0 12 3546.0 13 3688.5 14 4071.5 15 4535.5 16 4919.5 17 5350.5 18 5802.5 19 6298.0 20 6890.5 21 7695.5 22 8804.5 23 10216.5 24 12037.0 25 14319.0 26 17268.0 27 20853.0 28 25310.5 29 31124.0 30 38188.0 31 45279.5 32 53692.5 33 62379.0 34 70269.5 35 77608.5 36 86033.0 37 97502.0 38 105184.0 39 108055.0 40 109407.0 41 111570.5 42 116592.5 43 123507.5 44 132556.5 45 143843.0 46 154066.5 47 163023.5 48 170799.0 49 173956.0 50 172331.5 51 170709.0 52 167642.0 53 160452.5 54 151557.5 55 138102.5 56 123488.5 57 107140.5 58 90530.5 59 78010.5 60 63873.0 61 49510.0 62 38669.0 63 30143.0 64 22095.5 65 15518.0 66 11296.5 67 8580.0 68 6509.0 69 4841.0 70 3754.0 71 2814.0 72 2165.0 73 1723.5 74 1313.5 75 958.0 76 681.5 77 468.0 78 316.5 79 222.5 80 172.5 81 127.5 82 92.5 83 66.5 84 50.0 85 39.0 86 32.5 87 25.0 88 17.5 89 16.0 90 15.0 91 9.5 92 8.5 93 7.5 94 4.0 95 4.0 96 4.0 97 2.5 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2186486775971819 2 0.1447981284854406 3 0.023599141922186397 4 0.022397913065065563 5 0.027227854094738917 6 0.05380504255853738 7 0.061112518106022466 8 0.05375499135615735 9 0.0415925491778089 10-11 0.04306905964801992 12-13 0.04129224196352869 14-15 0.019507456127618554 16-17 0.018168586463952623 18-19 0.018356278472877754 20-21 0.02107155620199464 22-23 0.024074628344796727 24-25 0.020758736187119424 26-27 0.0185689960829929 28-29 0.01788079205026742 30-31 0.016216589571131267 32-33 0.02456262756800207 34-35 0.025200780398347514 36-37 0.02384939793408657 38-39 0.022160169853760396 40-41 0.023636680323971423 42-43 0.029605286207790567 44-45 0.03901491225523711 46-47 0.04415767329978568 48-49 0.0400159363028378 50-51 0.026614726865583492 52-53 0.04261859882659961 54-55 0.041342293165908726 56-57 0.04395746849026554 58-59 0.04666023341878742 60-61 0.023661705925161442 62-63 0.024950524386447334 64-65 0.02566375402036283 66-67 0.030693899859556326 68-69 0.03462291924638906 70-71 0.03897737385345208 72-73 0.04276875243373972 74-75 0.023911961937061615 76-77 0.01773063844312732 78-79 0.016979870407426798 80-81 0.020420890571054187 82-83 0.01818109926454763 84-85 0.011173930931342764 86-87 0.012662954202148798 88-89 0.018493919279422847 90-91 0.01903196970500822 92-93 0.019082020907388256 94-95 0.02261063067518071 96-97 0.023398937112666256 98-99 0.02890456937447008 100 0.028879543773280066 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3995908.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.72256946477263 #Duplication Level Percentage of deduplicated Percentage of total 1 77.86901586554413 50.39882788511162 2 13.639908286425536 17.656198231226096 3 3.999054074720079 7.764871654333572 4 1.625352484507413 4.207879563330874 5 0.8751399380715026 2.8320652716614823 6 0.5009659626131375 1.945428258882929 7 0.323243637703031 1.4644811156696158 8 0.22066642604634087 1.1425678470661929 9 0.16058385953335694 0.9354060003212086 >10 0.7555482934875091 8.05743117742212 >50 0.022438474554325856 0.970209057375286 >100 0.007118338139128841 0.828393214799973 >500 5.698483028982922E-4 0.2527838698957267 >1k 2.630069011885083E-4 0.4655081529040249 >5k 4.383448353141794E-5 0.2159869312566028 >10k+ 8.766896706283589E-5 0.8619617687426908 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16315 0.4082926834151337 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14067 0.3520351319399746 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7613 0.19051990185960238 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.0051202380034774E-5 5.0051202380034774E-5 10-11 1.2512800595008694E-5 0.0 0.0 1.7517920833012173E-4 1.0010240476006955E-4 12-13 2.5025601190017387E-5 0.0 0.0 3.25332815470226E-4 1.62666407735113E-4 14-15 2.5025601190017387E-5 0.0 0.0 4.6297362201532167E-4 1.876920089251304E-4 16-17 3.753840178502608E-5 0.0 0.0 5.630760267753912E-4 2.7528161309019124E-4 18-19 5.0051202380034774E-5 0.0 0.0 7.632808362955303E-4 3.0030721428020863E-4 20-21 5.0051202380034774E-5 0.0 0.0 0.0011261520535507824 3.0030721428020863E-4 22-23 5.0051202380034774E-5 0.0 0.0 0.0028779441368519997 3.0030721428020863E-4 24-25 5.0051202380034774E-5 0.0 0.0 0.007495167556410208 3.0030721428020863E-4 26-27 5.0051202380034774E-5 2.5025601190017387E-5 0.0 0.015841205553281005 3.0030721428020863E-4 28-29 5.0051202380034774E-5 2.5025601190017387E-5 0.0 0.04718577104377779 3.0030721428020863E-4 30-31 7.507680357005216E-5 2.5025601190017387E-5 0.0 0.14506089729793578 3.0030721428020863E-4 32-33 7.507680357005216E-5 2.5025601190017387E-5 0.0 0.3557639465172872 3.0030721428020863E-4 34-35 7.507680357005216E-5 2.5025601190017387E-5 0.0 0.6767923585828303 4.379480208253043E-4 36-37 7.507680357005216E-5 2.5025601190017387E-5 0.0 1.1713107508981688 4.50460821420313E-4 38-39 7.507680357005216E-5 2.5025601190017387E-5 0.0 1.8229899186868166 4.879992232053391E-4 40-41 7.507680357005216E-5 2.5025601190017387E-5 0.0 2.4687004805916453 5.255376249903651E-4 42-43 7.507680357005216E-5 1.0010240476006955E-4 0.0 3.087233239604115 5.380504255853738E-4 44-45 7.507680357005216E-5 1.0010240476006955E-4 0.0 3.831644772602372 5.505632261803825E-4 46-47 7.507680357005216E-5 1.0010240476006955E-4 0.0 4.700896517137031 6.006144285604173E-4 48-49 7.507680357005216E-5 1.0010240476006955E-4 0.0 5.577906198040596 6.256400297504347E-4 50-51 7.507680357005216E-5 1.0010240476006955E-4 0.0 6.424797567911973 6.256400297504347E-4 52-53 7.507680357005216E-5 1.0010240476006955E-4 0.0 7.2080112955553535 6.50665630940452E-4 54-55 7.507680357005216E-5 1.0010240476006955E-4 0.0 8.05987024726295 6.50665630940452E-4 56-57 7.507680357005216E-5 1.0010240476006955E-4 0.0 9.019627078501307 6.50665630940452E-4 58-59 7.507680357005216E-5 1.0010240476006955E-4 0.0 10.044488011235494 6.50665630940452E-4 60-61 7.507680357005216E-5 1.0010240476006955E-4 0.0 11.037754122467284 6.50665630940452E-4 62-63 7.507680357005216E-5 1.0010240476006955E-4 0.0 12.008009193404853 6.50665630940452E-4 64-65 7.507680357005216E-5 1.0010240476006955E-4 0.0 12.965551258937893 6.631784315354607E-4 66-67 7.507680357005216E-5 1.0010240476006955E-4 0.0 13.994166031850582 6.756912321304694E-4 68-69 8.758960416506085E-5 1.0010240476006955E-4 0.0 15.099772066824361 6.756912321304694E-4 70-71 1.0010240476006955E-4 1.0010240476006955E-4 0.0 16.21764064638125 6.756912321304694E-4 72-73 1.0010240476006955E-4 1.0010240476006955E-4 0.0 17.274409220632705 7.132296339154955E-4 74-75 1.0010240476006955E-4 1.0010240476006955E-4 0.0 18.278048443557758 7.257424345105042E-4 76-77 1.0010240476006955E-4 1.3764080654509565E-4 0.0 19.340109932460905 7.257424345105042E-4 78-79 1.0010240476006955E-4 1.5015360714010432E-4 0.0 20.431476400357568 7.257424345105042E-4 80-81 1.0010240476006955E-4 1.5015360714010432E-4 0.0 21.53982273866165 7.257424345105042E-4 82-83 1.0010240476006955E-4 1.751792083301217E-4 0.0 22.625295677478057 7.382552351055129E-4 84-85 1.0010240476006955E-4 1.751792083301217E-4 0.0 23.64331210828678 7.507680357005216E-4 86-87 1.0010240476006955E-4 1.751792083301217E-4 0.0 24.667534888190616 7.507680357005216E-4 88 1.0010240476006955E-4 1.751792083301217E-4 0.0 25.44790821009893 7.507680357005216E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7100 0.0 55.1317 1 GTATCAA 12835 0.0 40.333 1 TACCTGG 2935 0.0 33.353245 2 ATCAACG 15485 0.0 32.81643 3 TATCAAC 15640 0.0 32.679543 2 CAACGCA 15515 0.0 32.60107 5 TCAACGC 16315 0.0 31.089317 4 AACGCAG 16590 0.0 30.885664 6 GTGGTAT 2790 0.0 30.727406 1 ACGCAGA 18640 0.0 26.985195 7 TAACGCC 760 0.0 26.596941 4 CGCAGAG 18910 0.0 26.499794 8 ACCTGGG 3675 0.0 26.09454 3 GTACATG 12995 0.0 25.663488 1 CTAACGC 770 0.0 25.641024 3 TCTAACG 780 0.0 25.341805 2 GTACTAG 1590 0.0 25.181437 1 TACATGG 13120 0.0 24.823048 2 GCAGAGT 20140 0.0 24.132967 9 ACATGGG 13320 0.0 23.716024 3 >>END_MODULE