FastQCFastQC Report
Mon 6 Jun 2016
ERR1211455_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211455_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3995908
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149490.37410771218956995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137380.34380170914845887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76130.19051990185960238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68800.053.2327081
GTATCAA123800.041.3938141
TACCTGG29950.035.616562
ATCAACG151800.033.0936933
CAACGCA153100.032.684185
TATCAAC157300.032.532852
AACGCAG163700.031.1123586
TCAACGC163650.030.7264584
ACCTGGG35050.029.8989623
GTACTAG15050.027.4900651
ACGCAGA183800.027.2498447
GTGGTAT27100.026.8900721
CGCAGAG186600.026.6898758
GTACATG125050.025.1895641
GCAGAGT201900.024.2484369
TACATGG130050.023.9205192
ACATGGG127150.023.2843153
TAGGCAT21250.023.216335
CCTGGGG35050.022.7932174
TAGTACT18800.022.2472764
Produced by FastQC (version 0.11.5)