FastQCFastQC Report
Mon 6 Jun 2016
ERR1211454_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211454_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4063637
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180940.4452661495108938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148740.3660267883179526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84430.2077695424074542No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54940.13519908397329783No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43880.10798208599833105No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40920.10069797080792403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73450.054.2771031
TACCTGG33200.045.7896082
GTATCAA133400.040.623761
ACCTGGG41950.036.082963
CAACGCA163050.032.5788575
ATCAACG162050.032.576843
TATCAAC166750.032.1484762
TCAACGC169100.031.3298664
AACGCAG173850.030.9609556
GTACATG139200.030.2647021
TACATGG140300.029.3865722
ACATGGG142550.027.8655573
ACGCAGA193050.027.4677377
CGCAGAG197200.026.8403578
GTGGTAT27900.026.348661
CATGGGG112650.025.997714
ATGGGGG91650.025.7483545
CCTGGGG45500.025.4156914
CTAACGC6950.025.0262133
TCTAACG7300.024.499842
Produced by FastQC (version 0.11.5)