FastQCFastQC Report
Mon 6 Jun 2016
ERR1211454_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211454_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4063637
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166230.40906705003424276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143990.35433775211713053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85170.20959057120505595No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49160.12097537255419222No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45530.11204248804703766No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76100.054.7448731
TACCTGG38500.048.3415262
GTATCAA134650.040.2640381
ACCTGGG45400.039.855733
ATCAACG168100.031.6493343
TATCAAC172600.031.2598552
CAACGCA171150.031.1058945
CCTGGGG45250.030.951634
TCAACGC175650.030.4227314
AACGCAG182950.029.4063646
GTACATG141700.029.1020871
TGGGGGG96400.028.0746086
TACATGG143600.028.0486682
CTGGGGG32950.027.8079435
ACATGGG145150.026.551113
ACGCAGA202050.026.4870227
ATGGGGG91750.026.4773275
CATGGGG115800.026.218624
GTACCTG71800.026.1302051
CGCAGAG204900.025.9810228
Produced by FastQC (version 0.11.5)