##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211454_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4063637 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.064254755038405 33.0 31.0 34.0 30.0 34.0 2 32.19628623324377 33.0 31.0 34.0 30.0 34.0 3 32.2733627536121 34.0 31.0 34.0 30.0 34.0 4 35.918971847140874 37.0 35.0 37.0 35.0 37.0 5 35.77748061650192 37.0 35.0 37.0 35.0 37.0 6 35.81578325032477 37.0 35.0 37.0 35.0 37.0 7 35.75076956923072 37.0 35.0 37.0 33.0 37.0 8 35.73428901252745 37.0 35.0 37.0 33.0 37.0 9 37.31490386567501 39.0 37.0 39.0 34.0 39.0 10-11 37.29837286155235 39.0 37.0 39.0 34.0 39.0 12-13 37.20765781982987 39.0 37.0 39.0 33.0 39.0 14-15 38.407048168918635 40.0 38.0 41.0 33.5 41.0 16-17 38.38736678497612 40.0 38.0 41.0 33.5 41.0 18-19 38.39619286860514 40.0 38.0 41.0 33.5 41.0 20-21 38.35574351252339 40.0 38.0 41.0 34.0 41.0 22-23 38.25076624708358 40.0 38.0 41.0 34.0 41.0 24-25 38.13234006876107 40.0 37.0 41.0 33.0 41.0 26-27 37.99980866893377 40.0 37.0 41.0 33.0 41.0 28-29 37.90758229635176 40.0 37.0 41.0 33.0 41.0 30-31 37.93751804110455 40.0 37.0 41.0 33.0 41.0 32-33 37.755265910808475 40.0 37.0 41.0 33.0 41.0 34-35 37.6384515890568 40.0 36.5 41.0 33.0 41.0 36-37 37.488486422384675 40.0 36.0 41.0 32.0 41.0 38-39 37.36910949477033 40.0 36.0 41.0 32.0 41.0 40-41 37.288147046598894 40.0 36.0 41.0 32.0 41.0 42-43 37.14552185640598 39.0 36.0 41.0 31.0 41.0 44-45 36.98508171374559 39.0 35.5 41.0 31.0 41.0 46-47 36.754667309112506 39.0 35.0 41.0 30.5 41.0 48-49 36.5245760140485 39.0 35.0 41.0 30.0 41.0 50-51 36.335099567210364 39.0 35.0 41.0 29.5 41.0 52-53 36.12321142365816 39.0 35.0 40.0 29.0 41.0 54-55 35.9164478766189 38.0 35.0 40.0 28.0 41.0 56-57 35.680717175279185 38.0 34.5 40.0 28.0 41.0 58-59 35.41406589712615 38.0 34.0 40.0 27.5 41.0 60-61 35.14289206934576 37.5 34.0 40.0 27.0 41.0 62-63 34.829437644159654 37.0 34.0 40.0 26.0 41.0 64-65 34.46701341679879 36.0 33.5 39.0 26.0 41.0 66-67 34.105001258724634 36.0 33.0 39.0 26.0 40.5 68-69 33.72570372304416 35.0 33.0 39.0 25.5 40.0 70-71 33.337838369913456 35.0 32.5 38.0 25.0 40.0 72-73 32.96947894213976 35.0 32.0 37.0 24.5 39.5 74-75 32.592329728270514 35.0 32.0 37.0 24.0 39.0 76-77 31.564862707963336 34.0 30.5 35.5 22.5 38.0 78-79 32.02655466027109 34.5 31.0 36.0 24.0 37.5 80-81 31.920691119802285 35.0 31.0 35.0 23.5 37.0 82-83 31.656480881535433 34.0 31.0 35.0 23.0 37.0 84-85 31.38939870859528 34.0 31.0 35.0 22.5 36.0 86-87 31.146793746587107 34.0 31.0 35.0 20.5 36.0 88-89 30.92317953104571 34.0 30.5 35.0 20.0 35.5 90-91 30.707927036789947 34.0 30.0 35.0 20.0 35.0 92-93 30.53377811551573 34.0 30.0 35.0 19.5 35.0 94-95 30.368789092135934 34.0 30.0 35.0 19.0 35.0 96-97 30.20097648977997 34.0 29.0 35.0 18.5 35.0 98-99 30.00039152119148 34.0 29.0 35.0 18.0 35.0 100 28.2112843740718 32.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.266836701257013 1101 2 -0.2067709537455258 1101 3 -0.30488001516238583 1101 4 -0.20562678244799315 1101 5 -0.20505876167347026 1101 6 -0.23995065871405785 1101 7 -0.17814451366287187 1101 8 -0.3122985714734696 1101 9 -0.4556455302828297 1101 10-11 -0.4399939174056655 1101 12-13 -0.44987338177276825 1101 14-15 -0.6567877196101364 1101 16-17 -0.6642185613979734 1101 18-19 -0.6256167325633868 1101 20-21 -0.707707914114124 1101 22-23 -0.72307208228451 1101 24-25 -0.7970975566147658 1101 26-27 -0.685475605624994 1101 28-29 -0.6791352961332606 1101 30-31 -0.6315421016415641 1101 32-33 -0.5258845856204672 1101 34-35 -0.7096410505741346 1101 36-37 -0.7159340742200797 1101 38-39 -0.750921237234337 1101 40-41 -0.583350164212014 1101 42-43 -0.5486186304921574 1101 44-45 -0.655658172421802 1101 46-47 -0.5908353192401705 1101 48-49 -0.6035955435278737 1101 50-51 -0.5259288972143779 1101 52-53 -0.6409035660674078 1101 54-55 -0.7030182791815847 1101 56-57 -0.6894949701559554 1101 58-59 -0.6771351252981361 1101 60-61 -0.5707479598985614 1101 62-63 -0.5867949295252046 1101 64-65 -0.6850942269944582 1101 66-67 -0.6390289817843566 1101 68-69 -0.624994322361168 1101 70-71 -0.7939589566920233 1101 72-73 -0.79080990463099 1101 74-75 -0.8550042965164266 1101 76-77 -0.6611680094671897 1101 78-79 -0.5372695089956103 1101 80-81 -0.5655009166024634 1101 82-83 -0.6160913688963738 1101 84-85 -0.6829378750043382 1101 86-87 -0.5675694791145425 1101 88-89 -0.7322153032335557 1101 90-91 -0.8027069860752398 1101 92-93 -1.0221083544331613 1101 94-95 -1.0229667301672052 1101 96-97 -0.9847764178487601 1101 98-99 -1.0896310688269182 1101 100 -1.0637380289660499 1102 1 -0.2898332672937549 1102 2 -0.21217713093460944 1102 3 -0.25091738633858185 1102 4 -0.15259571444938302 1102 5 -0.22494733219713936 1102 6 -0.34917036175787786 1102 7 -0.2634565119201895 1102 8 -0.34958736117662426 1102 9 -0.3950826437743302 1102 10-11 -0.466532098962972 1102 12-13 -0.4851763864622498 1102 14-15 -0.6470265784734366 1102 16-17 -0.6462456077313874 1102 18-19 -0.6889109843862542 1102 20-21 -0.6877027021212001 1102 22-23 -0.7137103061783563 1102 24-25 -0.7954140881364609 1102 26-27 -0.8129067817035107 1102 28-29 -0.8783538637445929 1102 30-31 -0.7679168812860624 1102 32-33 -0.7105109257864299 1102 34-35 -0.6985505981474915 1102 36-37 -0.7718160726755059 1102 38-39 -0.7272390043788661 1102 40-41 -0.8454022651155739 1102 42-43 -0.901325851502385 1102 44-45 -0.821910884783243 1102 46-47 -0.8209352503898444 1102 48-49 -0.8661423997544517 1102 50-51 -1.0275435502804058 1102 52-53 -0.9763255948016152 1102 54-55 -0.9330786316509005 1102 56-57 -0.814504472093823 1102 58-59 -0.9768773445964953 1102 60-61 -0.9853280911915618 1102 62-63 -0.9175051316348046 1102 64-65 -0.9600526431979262 1102 66-67 -0.9151214777276593 1102 68-69 -1.0667053117679615 1102 70-71 -1.0459794548702277 1102 72-73 -1.167623814752389 1102 74-75 -1.0244156232103734 1102 76-77 -0.8621285626575741 1102 78-79 -0.8574055269998517 1102 80-81 -0.8502380159817804 1102 82-83 -0.8015051807998042 1102 84-85 -0.8643536292740563 1102 86-87 -0.8350948707798409 1102 88-89 -0.8616645556415925 1102 90-91 -0.873636781350914 1102 92-93 -0.977776305101461 1102 94-95 -1.015327172118372 1102 96-97 -1.2208429114281536 1102 98-99 -1.2381906274098675 1102 100 -1.258928211288925 1103 1 -0.3482279332516818 1103 2 -0.25774548354943505 1103 3 -0.27649588042983453 1103 4 -0.17002305913751314 1103 5 -0.1936954182146593 1103 6 -0.27596524030369096 1103 7 -0.33786441969477465 1103 8 -0.26802484192941733 1103 9 -0.45457959183933383 1103 10-11 -0.5162273521489809 1103 12-13 -0.5807956655068764 1103 14-15 -0.7437524346769848 1103 16-17 -0.820784746520232 1103 18-19 -0.6426830406586461 1103 20-21 -0.664154307237915 1103 22-23 -0.7187446653046479 1103 24-25 -0.7408963077814619 1103 26-27 -0.8179504181418125 1103 28-29 -0.8722537734024698 1103 30-31 -0.7474788770523375 1103 32-33 -0.7065008506256092 1103 34-35 -0.7259577535148907 1103 36-37 -0.699616379909159 1103 38-39 -0.7909360836491146 1103 40-41 -0.8383848375990866 1103 42-43 -0.7880989214929173 1103 44-45 -0.6822918060598369 1103 46-47 -0.8091458055141274 1103 48-49 -0.7562561181242984 1103 50-51 -0.809249257966222 1103 52-53 -0.9043385930029331 1103 54-55 -0.6669291293142621 1103 56-57 -0.8474712284783976 1103 58-59 -0.9389808565487172 1103 60-61 -0.9134614796962737 1103 62-63 -0.9309464964029956 1103 64-65 -0.8351023691607296 1103 66-67 -0.9277075258702041 1103 68-69 -0.9299138804307674 1103 70-71 -0.6755368162755602 1103 72-73 -0.8618823956002579 1103 74-75 -0.8747048906574975 1103 76-77 -0.797363046288833 1103 78-79 -0.6235251357756049 1103 80-81 -0.5572561650669812 1103 82-83 -0.5367357215734643 1103 84-85 -0.7021087757555122 1103 86-87 -0.7534284112778415 1103 88-89 -0.6899289260475534 1103 90-91 -0.8529118247030212 1103 92-93 -0.8209767183198089 1103 94-95 -0.8958691956358429 1103 96-97 -0.8580670262888361 1103 98-99 -1.186250522423336 1103 100 -1.2258424819680762 1104 1 -0.4388320942832671 1104 2 -0.34895685180813985 1104 3 -0.3707656355118125 1104 4 -0.29284748383894055 1104 5 -0.27706023215290543 1104 6 -0.35845472003820333 1104 7 -0.3557408247809164 1104 8 -0.3851103415184909 1104 9 -0.41115163680208866 1104 10-11 -0.4675155320000073 1104 12-13 -0.6063456712286523 1104 14-15 -0.6390583188734453 1104 16-17 -0.7324503181386604 1104 18-19 -0.7641215601751057 1104 20-21 -0.6383202852324814 1104 22-23 -0.6738978479775497 1104 24-25 -0.8575567379289808 1104 26-27 -0.8811349993858286 1104 28-29 -0.8942530269707802 1104 30-31 -0.7750412167775877 1104 32-33 -0.7673146334738021 1104 34-35 -0.7837183511544055 1104 36-37 -0.7068927478687641 1104 38-39 -0.8910737069924011 1104 40-41 -1.0046980112081698 1104 42-43 -0.6683216676333217 1104 44-45 -0.923431709010714 1104 46-47 -0.8479878870962594 1104 48-49 -0.7787782965084702 1104 50-51 -0.8178614249328433 1104 52-53 -0.7887667632569801 1104 54-55 -0.7357704257776874 1104 56-57 -0.7703515624090471 1104 58-59 -0.8630610566678385 1104 60-61 -0.8307217128976561 1104 62-63 -0.8769932774488254 1104 64-65 -0.9174281605863897 1104 66-67 -0.8334365110221924 1104 68-69 -0.8251338973490192 1104 70-71 -0.7986048228347968 1104 72-73 -0.8042742296505452 1104 74-75 -0.8007848677573897 1104 76-77 -0.7474015788743493 1104 78-79 -0.6838272097893743 1104 80-81 -0.7326307138269179 1104 82-83 -0.9223097099894666 1104 84-85 -0.7445094119129791 1104 86-87 -0.6561602911283444 1104 88-89 -0.762472349776278 1104 90-91 -0.8175461284408989 1104 92-93 -0.9902388010202792 1104 94-95 -1.0630946836773312 1104 96-97 -1.1501248028976967 1104 98-99 -1.0637619269633092 1104 100 -1.3695748777537275 1105 1 -0.38386472458143217 1105 2 -0.359015511382502 1105 3 -0.3630456768216348 1105 4 -0.17996412275095963 1105 5 -0.23482654668246283 1105 6 -0.36090400585160864 1105 7 -0.31482757602491773 1105 8 -0.3150732971160082 1105 9 -0.5346861541685684 1105 10-11 -0.4923571818156347 1105 12-13 -0.48242952743974143 1105 14-15 -0.6415132578379925 1105 16-17 -0.6615826089999501 1105 18-19 -0.6155626335861513 1105 20-21 -0.675752160889175 1105 22-23 -0.7063023685779442 1105 24-25 -0.689161213737826 1105 26-27 -0.6742044993808705 1105 28-29 -0.6691259627187733 1105 30-31 -0.6643411723577586 1105 32-33 -0.6848542860698501 1105 34-35 -0.6522321733412682 1105 36-37 -0.7405679884297101 1105 38-39 -0.6868393341607373 1105 40-41 -0.6876748707696478 1105 42-43 -0.5182449086824477 1105 44-45 -0.671221597954947 1105 46-47 -0.7441387261787682 1105 48-49 -0.6600390452396852 1105 50-51 -0.7029217881965977 1105 52-53 -0.7161003066087517 1105 54-55 -0.7892464864637248 1105 56-57 -0.8255671673984466 1105 58-59 -0.8481360019639155 1105 60-61 -0.8765217236154044 1105 62-63 -0.7938024732207083 1105 64-65 -0.8258568695491633 1105 66-67 -0.7730710402215735 1105 68-69 -0.8621716786746063 1105 70-71 -0.842731694415022 1105 72-73 -0.9198647918928273 1105 74-75 -0.8788763747728723 1105 76-77 -0.8975085783118182 1105 78-79 -0.8937825328584452 1105 80-81 -0.7612578065770066 1105 82-83 -0.7919948960695216 1105 84-85 -0.6672681620616601 1105 86-87 -0.7642113308744882 1105 88-89 -0.7971514893078187 1105 90-91 -0.7081362028084932 1105 92-93 -0.7779156594666112 1105 94-95 -0.8215862587784386 1105 96-97 -0.9244639805051449 1105 98-99 -0.8786833342231297 1105 100 -0.9243944136811315 1106 1 -0.30674706061420665 1106 2 -0.3051040176740827 1106 3 -0.3881933230069876 1106 4 -0.1767109360269501 1106 5 -0.23217950889520722 1106 6 -0.23057681137878916 1106 7 -0.30142549932941876 1106 8 -0.3473280676993227 1106 9 -0.41141030489655606 1106 10-11 -0.49644053392946574 1106 12-13 -0.5931885698548811 1106 14-15 -0.6726280410795837 1106 16-17 -0.6317552456151247 1106 18-19 -0.6971602892404789 1106 20-21 -0.574084902693123 1106 22-23 -0.5227549600839438 1106 24-25 -0.6546432247218306 1106 26-27 -0.6652157267436252 1106 28-29 -0.6972944572787227 1106 30-31 -0.6396084964092594 1106 32-33 -0.7104343334911647 1106 34-35 -0.7238947217938261 1106 36-37 -0.7389545526605872 1106 38-39 -0.7335548616047731 1106 40-41 -0.6421321684204884 1106 42-43 -0.5410045079898822 1106 44-45 -0.7434132615739983 1106 46-47 -0.7175763576141279 1106 48-49 -0.8394643035895797 1106 50-51 -0.886578955755958 1106 52-53 -0.8483229816620934 1106 54-55 -0.9084588886983056 1106 56-57 -1.0352885143221116 1106 58-59 -0.9572214435484838 1106 60-61 -0.9991023571681268 1106 62-63 -0.8371228918705711 1106 64-65 -0.9712127340794794 1106 66-67 -0.8541419423286314 1106 68-69 -0.8368382517761646 1106 70-71 -0.831996673041246 1106 72-73 -0.7506657946588433 1106 74-75 -0.7903554636466765 1106 76-77 -0.912939285929415 1106 78-79 -0.7468350494223941 1106 80-81 -0.622205666072805 1106 82-83 -0.7100934911984673 1106 84-85 -0.6747757496068871 1106 86-87 -0.7508080171403506 1106 88-89 -0.8075543095759983 1106 90-91 -0.8168414236963635 1106 92-93 -0.9365333654931511 1106 94-95 -0.8293764534253434 1106 96-97 -0.8980547798165439 1106 98-99 -0.9518394381541668 1106 100 -0.9756874339492221 1107 1 -0.3950130744650977 1107 2 -0.37526655970989253 1107 3 -0.4509744463035936 1107 4 -0.2374839617431448 1107 5 -0.2589719895622764 1107 6 -0.3349847331747071 1107 7 -0.4038037364780749 1107 8 -0.4319623792127061 1107 9 -0.5273686655889946 1107 10-11 -0.5058488495821294 1107 12-13 -0.47256447202629204 1107 14-15 -0.6477462660108202 1107 16-17 -0.652122893378241 1107 18-19 -0.6638614880660825 1107 20-21 -0.7139470085129247 1107 22-23 -0.6597327264057995 1107 24-25 -0.7418039021211271 1107 26-27 -0.6383213150919786 1107 28-29 -0.7097444764283054 1107 30-31 -0.751960887691574 1107 32-33 -0.7273304663897235 1107 34-35 -0.7775803274661612 1107 36-37 -0.7687333752905232 1107 38-39 -0.6880611189234713 1107 40-41 -0.7587376050160586 1107 42-43 -0.6931029424414703 1107 44-45 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0.9740470864035444 2311 34-35 0.9134361498505399 2311 36-37 0.9454089280099112 2311 38-39 1.0786516476380896 2311 40-41 1.0346205571282567 2311 42-43 1.0826526804392387 2311 44-45 0.9885550672338752 2311 46-47 1.0920518598631332 2311 48-49 1.097354685093265 2311 50-51 1.086803447644975 2311 52-53 1.0946750367584812 2311 54-55 1.1063644513739348 2311 56-57 1.159274537557451 2311 58-59 1.1808516433553606 2311 60-61 1.2519297780964749 2311 62-63 1.2051178932505096 2311 64-65 1.1997537026892218 2311 66-67 1.1709406067756802 2311 68-69 1.1749202554674127 2311 70-71 1.2117542687184937 2311 72-73 1.2234787868022536 2311 74-75 1.1847221072864116 2311 76-77 1.0804136025959714 2311 78-79 1.0241683227039253 2311 80-81 1.0127640823491326 2311 82-83 0.9933588538375808 2311 84-85 1.07425701511238 2311 86-87 1.0588837638989688 2311 88-89 1.0711126230386085 2311 90-91 1.1186191757489574 2311 92-93 1.277550298521053 2311 94-95 1.2366232385294325 2311 96-97 1.2675739882664985 2311 98-99 1.4179831384871164 2311 100 1.499342694009826 2312 1 0.1436945689693161 2312 2 0.15726248637913187 2312 3 0.2761459638380259 2312 4 0.19632022189671972 2312 5 0.30360723979222826 2312 6 0.4329568180807897 2312 7 0.446650286354938 2312 8 0.28662897648115404 2312 9 0.38680418049989385 2312 10-11 0.2627532899926308 2312 12-13 0.42414822053004997 2312 14-15 0.7648897258213694 2312 16-17 0.8228888033656148 2312 18-19 0.8867577519612198 2312 20-21 0.8591888483695271 2312 22-23 0.5017946868683723 2312 24-25 0.4152185875222756 2312 26-27 0.758359317277872 2312 28-29 0.8626113225720289 2312 30-31 0.816047676502599 2312 32-33 0.7630315156622842 2312 34-35 0.9027233292428178 2312 36-37 0.9190745372294913 2312 38-39 0.8758217172075149 2312 40-41 0.9102931567053858 2312 42-43 0.8597137126659362 2312 44-45 0.7187304404890469 2312 46-47 0.7995812481014681 2312 48-49 0.967507495707423 2312 50-51 0.9486989690969594 2312 52-53 0.8891554791281209 2312 54-55 0.8427014330796396 2312 56-57 0.8775674468015566 2312 58-59 0.8620851319899856 2312 60-61 0.9745905041415952 2312 62-63 1.0069797095405377 2312 64-65 1.0648060789635423 2312 66-67 1.0175397731557538 2312 68-69 1.047672345043516 2312 70-71 0.8626899962929784 2312 72-73 0.9558310642204262 2312 74-75 1.0437909369644345 2312 76-77 0.9566932575093965 2312 78-79 0.9062932387173355 2312 80-81 0.7618172251055135 2312 82-83 0.8621852932019571 2312 84-85 0.702658568433776 2312 86-87 0.7839226534818522 2312 88-89 0.4684438029223337 2312 90-91 0.8591389724230112 2312 92-93 1.0732411245378515 2312 94-95 1.1810445497124107 2312 96-97 1.1937162316068175 2312 98-99 1.1616260539771979 2312 100 1.4096923095607217 2313 1 -0.46920652838789323 2313 2 -0.04026799994697683 2313 3 0.19328821886444558 2313 4 0.04257756646286737 2313 5 0.18867951885312806 2313 6 0.31925214615898057 2313 7 0.04351643754302614 2313 8 0.016372775936233097 2313 9 0.18299763382164969 2313 10-11 0.28790448812072356 2313 12-13 0.49372034989810487 2313 14-15 0.4655655178971827 2313 16-17 0.6937298095021802 2313 18-19 0.6029527607504477 2313 20-21 0.7889091067443772 2313 22-23 0.8110080719168096 2313 24-25 0.7245145963265571 2313 26-27 0.8757414153526 2313 28-29 0.7449037889245957 2313 30-31 0.7509699282173798 2313 32-33 0.8643810091318329 2313 34-35 0.8399462929602919 2313 36-37 0.6248873860597968 2313 38-39 0.4249014407691618 2313 40-41 0.5915356407032988 2313 42-43 0.6276384681815657 2313 44-45 0.7754644020305363 2313 46-47 0.8539455887588758 2313 48-49 0.8671614041957554 2313 50-51 0.8065294515762034 2313 52-53 0.7918929764311429 2313 54-55 0.8089663542776506 2313 56-57 0.8226128647790816 2313 58-59 0.6894552128241145 2313 60-61 0.7642304217854488 2313 62-63 0.7943531605541878 2313 64-65 0.9474438841822206 2313 66-67 0.9715783437313306 2313 68-69 0.981736622645947 2313 70-71 0.8826255280203341 2313 72-73 0.9297372611693717 2313 74-75 0.6331440966540853 2313 76-77 0.7591797057081457 2313 78-79 0.5902162698383897 2313 80-81 0.7239321005593986 2313 82-83 0.7057390513284396 2313 84-85 0.7658433295357128 2313 86-87 0.941507355375677 2313 88-89 0.9690723873967606 2313 90-91 1.0071355554122157 2313 92-93 0.860252427601452 2313 94-95 0.9310663764333356 2313 96-97 1.1032961132240366 2313 98-99 1.0916675247469527 2313 100 1.222699864449492 2314 1 -0.4314530308432296 2314 2 -0.23973072784395555 2314 3 0.11291132100529921 2314 4 -0.06260066222591121 2314 5 0.24955410286894875 2314 6 0.16872962285867033 2314 7 0.05608121836143454 2314 8 0.09429321873606256 2314 9 0.10825789623043391 2314 10-11 0.36394659695169906 2314 12-13 0.4914974775028327 2314 14-15 0.6148575753671537 2314 16-17 0.7748392312292083 2314 18-19 0.7924655226616082 2314 20-21 0.31457142391318627 2314 22-23 0.5478567763803852 2314 24-25 0.7889225378173776 2314 26-27 0.743165626891205 2314 28-29 0.6450019144277093 2314 30-31 0.658393292399289 2314 32-33 0.723173373968443 2314 34-35 0.7346756544290542 2314 36-37 0.6663142547810708 2314 38-39 0.7200375334476092 2314 40-41 0.6998671953869788 2314 42-43 0.4896882399948055 2314 44-45 0.6081367172088505 2314 46-47 0.7517273528435808 2314 48-49 0.71462349097947 2314 50-51 0.8061363500189103 2314 52-53 0.8066423786849555 2314 54-55 0.8497254899418536 2314 56-57 0.7629624713577599 2314 58-59 0.7587705885908349 2314 60-61 0.7414999587529749 2314 62-63 0.4651786698275444 2314 64-65 0.6812479401429314 2314 66-67 0.8482893047697075 2314 68-69 0.9025773203882252 2314 70-71 0.9892982620806379 2314 72-73 0.9260118894715674 2314 74-75 0.8796475112617088 2314 76-77 0.6792550245261033 2314 78-79 0.6567454009220732 2314 80-81 0.6987521771572496 2314 82-83 0.7023551803632735 2314 84-85 0.4981596349966111 2314 86-87 0.6946128776631575 2314 88-89 0.7262207203823614 2314 90-91 0.8578270704140287 2314 92-93 0.8899747424200406 2314 94-95 1.076995227249796 2314 96-97 1.1510756390080594 2314 98-99 1.1216597946156703 2314 100 1.2082491554277794 2315 1 -0.073616320473743 2315 2 0.13704510347978527 2315 3 0.1177029241504215 2315 4 -0.07671376290871024 2315 5 0.24414252947499904 2315 6 0.3725443801832853 2315 7 0.38891284700756756 2315 8 0.3414024111116305 2315 9 0.5223703039509573 2315 10-11 0.5357582775238043 2315 12-13 0.6143959539097779 2315 14-15 0.749900793114378 2315 16-17 0.6878944095053043 2315 18-19 0.7366415212202426 2315 20-21 0.7733385516307507 2315 22-23 0.8392702808292043 2315 24-25 0.7746497093301556 2315 26-27 0.843260678216609 2315 28-29 0.8663937753548012 2315 30-31 0.8392607194765631 2315 32-33 0.8876415023235396 2315 34-35 0.8336382899031847 2315 36-37 0.8597987924828345 2315 38-39 0.9332404281820175 2315 40-41 0.889221562435246 2315 42-43 0.9304549796535042 2315 44-45 0.7307628594416613 2315 46-47 0.7221273537675259 2315 48-49 0.8855619235911973 2315 50-51 0.7602389176751316 2315 52-53 0.7677354342790039 2315 54-55 0.695934870803157 2315 56-57 0.8132947467686904 2315 58-59 0.9047210325486716 2315 60-61 0.9183553652446435 2315 62-63 0.8978044886779699 2315 64-65 0.922150846888691 2315 66-67 0.8610192527513405 2315 68-69 0.8017411459095669 2315 70-71 0.3583377470710758 2315 72-73 0.6908438230211047 2315 74-75 0.8370038832808149 2315 76-77 0.8459809394672924 2315 78-79 0.7018909691973327 2315 80-81 0.7768039830169364 2315 82-83 0.7520401135155161 2315 84-85 0.7583889131147146 2315 86-87 0.7651244455134005 2315 88-89 0.7970795778261603 2315 90-91 0.9135018539788113 2315 92-93 1.0096534851092933 2315 94-95 1.11336442831049 2315 96-97 1.155701046700866 2315 98-99 1.1202237457941706 2315 100 1.0039634147435024 2316 1 0.5016944417114075 2316 2 0.4824291249022181 2316 3 0.4527608544818804 2316 4 0.31202862018461275 2316 5 0.383709364265151 2316 6 0.42853729769627336 2316 7 0.4768315391438023 2316 8 0.5024572908767126 2316 9 0.5631436719740179 2316 10-11 0.4642829749395716 2316 12-13 0.6331715813298828 2316 14-15 0.8124574682252543 2316 16-17 0.8186703260065116 2316 18-19 0.7977972940937903 2316 20-21 0.8380795958221299 2316 22-23 0.7904994460694823 2316 24-25 0.9383952304749172 2316 26-27 0.9480800595830488 2316 28-29 0.8906049833334393 2316 30-31 0.7478683945798323 2316 32-33 0.7297164960474376 2316 34-35 0.6601145053751054 2316 36-37 0.6557229391230308 2316 38-39 0.6249026255026919 2316 40-41 0.6924161938948714 2316 42-43 0.8344399246139744 2316 44-45 0.7465790190752912 2316 46-47 0.7684487016462072 2316 48-49 0.4969179612021719 2316 50-51 0.630113053284596 2316 52-53 0.8902049286153044 2316 54-55 0.8768137724471217 2316 56-57 0.905356332454609 2316 58-59 0.9451614818415592 2316 60-61 0.902206857435651 2316 62-63 0.9562519329727976 2316 64-65 0.9530980249246142 2316 66-67 0.8774890780094253 2316 68-69 0.7997552348096093 2316 70-71 0.6464697316128394 2316 72-73 0.7029165725627564 2316 74-75 0.7825315894222697 2316 76-77 0.6618183047212689 2316 78-79 0.6793905698089695 2316 80-81 0.6839159486923556 2316 82-83 0.617359510096751 2316 84-85 0.6946170513563352 2316 86-87 0.6542086860288805 2316 88-89 0.6413616677303828 2316 90-91 0.766031028347971 2316 92-93 0.7965740867022824 2316 94-95 0.7634947049915297 2316 96-97 1.011943581942134 2316 98-99 1.0249987189780896 2316 100 0.7142292172098195 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 23.0 13 139.0 14 597.0 15 1640.0 16 3871.0 17 7727.0 18 12569.0 19 17361.0 20 23204.0 21 29717.0 22 35255.0 23 37391.0 24 38233.0 25 41039.0 26 46990.0 27 56663.0 28 68514.0 29 82995.0 30 101262.0 31 124430.0 32 156236.0 33 202157.0 34 282589.0 35 386025.0 36 573127.0 37 879415.0 38 752312.0 39 102145.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.10609325138451 18.94888145167902 12.742102232172424 24.202923064764047 2 16.482156255590745 20.88003923578804 39.10925114620228 23.528553362418936 3 18.934811352490392 26.188190529813564 30.7493508893634 24.12764722833265 4 13.14646929303162 16.90393245899412 37.04609995584494 32.90349829212932 5 12.875016088509813 37.44846447324804 34.07193231185549 15.604587126386656 6 30.09993264654299 36.13666771909006 18.811744257668682 14.951655376698264 7 26.71079798111541 33.415862172491096 22.176526898364273 17.696812948029226 8 26.387566178580414 34.870088930428146 20.421564386092616 18.320780504898828 9 27.777475634019964 15.702359141195332 20.157497560633487 36.36266766415122 10-11 25.251903448915687 25.60042356421709 28.57318633269601 20.574486654171213 12-13 25.43847247697222 23.875828093860544 28.070310138153758 22.61538929101348 14-15 23.278182799613795 25.30049754708561 26.135205778175724 25.286113875124872 16-17 22.32845309499491 27.73439090902268 27.020842307752407 22.91631368823 18-19 21.875009997202014 27.095579649461797 29.0802303453778 21.94918000795839 20-21 22.773767138592138 26.692351381865898 28.632079743362844 21.901801736179127 22-23 22.69485438783041 26.314186035809794 28.599220353589654 22.391739222770145 24-25 22.17960301301704 26.003064498049717 29.004084890016564 22.813247598916682 26-27 22.19070556516381 26.242618679428965 28.76337841283028 22.803297342576943 28-29 21.974770652925816 27.41851377632794 28.527148703822053 22.07956686692419 30-31 23.213751134614043 26.42326465419326 28.77430430333252 21.588679907860172 32-33 22.981698567831714 26.41465689737071 28.437240805597735 22.16640372919984 34-35 22.85286387375533 26.68156065132815 28.670858938138398 21.794716536778125 36-37 22.43767524404778 26.502924831696173 28.7158569116234 22.34354301263265 38-39 22.435883156864207 26.561931772380976 28.691781955659863 22.310403115094957 40-41 22.392370966479756 25.84787409496032 29.3371154980985 22.42263944046142 42-43 22.09022957164738 26.34639065963705 28.591474290574194 22.971905478141377 44-45 21.25007106368118 26.759579814912026 29.22336687046869 22.766982250938103 46-47 21.21528981027164 26.018502748659145 28.916568547148565 23.84963889392066 48-49 21.522654506168433 26.23977772473009 28.909172792443822 23.328394976657652 50-51 21.393591448209158 26.440447499749276 28.820763220506773 23.34519783153479 52-53 21.157084330798963 26.71498967943804 28.371755834443245 23.75617015531975 54-55 20.84761784754992 26.292571133804447 28.91344827229775 23.946362746347884 56-57 21.319762851248257 26.110049020241167 28.28787465465641 24.28231347385417 58-59 20.758869654553504 26.34056771252673 28.545151590213536 24.35541104270623 60-61 21.50399321976422 26.396716389298046 28.11074877284795 23.988541618089783 62-63 21.42420426210487 26.336298887501915 27.851629188632497 24.387867661760716 64-65 20.94879492495309 26.015424113905993 27.76611248384451 25.26966847729641 66-67 21.08802940172859 26.071568727377652 27.448039072313225 25.392362798580532 68-69 20.815519384334163 26.28537429236642 27.482971489802594 25.416134833496823 70-71 21.030437002184684 26.215022363258395 27.358070278476827 25.39647035608009 72-73 21.063243978197114 26.716364301503358 26.66653701067274 25.553854709626783 74-75 21.354372756384894 26.771332089682204 26.363762909075334 25.51053224485757 76-77 21.106623833977007 26.708432682699346 26.37507666203853 25.80986682128511 78-79 20.946056947511494 26.944059717545166 26.53471986788502 25.57516346705832 80-81 20.948729918773175 27.094869300531876 26.1705317924509 25.785868988244047 82-83 21.30304071222236 26.765309928994128 26.340490699010218 25.5911586597733 84-85 21.400296968006387 27.070596006565694 25.812248859287834 25.71685816614008 86-87 20.905626828675004 27.27797140299442 25.994230471892937 25.822171296437634 88-89 20.89632741623962 26.762951993830438 26.170544968172187 26.170175621757753 90-91 21.49525601283526 26.258148968028166 26.116133987404755 26.130461031731816 92-93 21.311959032864266 26.3934547891872 26.05908545408471 26.235500723863826 94-95 20.860083719366617 26.173376709832336 26.322146058768936 26.644393512032106 96-97 20.803373454838084 26.1341359274909 26.443414404858878 26.619076212812136 98-99 20.682681769820892 26.352653482174226 26.26137244006643 26.703292307938447 100 20.612764486940993 26.485244652420235 26.412955780476143 26.489035080162626 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2690.0 1 1943.5 2 2412.5 3 3461.0 4 2431.0 5 1692.5 6 2234.5 7 3531.0 8 5154.0 9 5802.0 10 4759.5 11 3671.5 12 3716.5 13 4146.5 14 4593.0 15 5031.5 16 5524.0 17 6046.0 18 6562.5 19 7070.0 20 7707.5 21 8388.0 22 9365.5 23 10843.0 24 12481.5 25 14469.5 26 17215.0 27 20448.0 28 24171.5 29 29087.5 30 35343.0 31 41175.5 32 47860.0 33 54808.5 34 61683.5 35 68936.5 36 76103.5 37 85839.5 38 93492.0 39 96903.5 40 99127.5 41 102762.0 42 109463.5 43 117255.5 44 126376.0 45 138523.0 46 151339.5 47 164592.0 48 178393.5 49 189611.5 50 197129.0 51 201482.0 52 198286.0 53 186886.5 54 173172.5 55 154590.0 56 134422.0 57 115587.5 58 97090.0 59 79910.5 60 61878.5 61 46886.5 62 36532.0 63 28019.5 64 20401.5 65 14426.0 66 10567.0 67 8053.0 68 6065.5 69 4399.0 70 3311.0 71 2565.5 72 1989.0 73 1513.5 74 1132.0 75 838.5 76 617.0 77 380.5 78 211.5 79 133.5 80 88.5 81 63.0 82 45.0 83 27.5 84 11.5 85 8.5 86 7.5 87 4.0 88 3.5 89 2.5 90 0.5 91 0.5 92 2.0 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05113645731643845 2 0.0 3 0.0 4 0.016807603631918893 5 0.0043803125131501655 6 0.0 7 1.7225948085422E-4 8 2.706934699137743E-4 9 0.002017896775720863 10-11 6.521251775195472E-4 12-13 3.0760621581110714E-4 14-15 3.691274589733286E-5 16-17 8.612974042711E-5 18-19 0.0 20-21 2.460849726488857E-5 22-23 0.0 24-25 6.152124316222142E-5 26-27 2.460849726488857E-5 28-29 8.859059015359886E-4 30-31 7.505591665791014E-4 32-33 0.01395301794919182 34-35 0.02271364297549215 36-37 0.031166661785981375 38-39 0.020658833453873954 40-41 7.382549179466572E-5 42-43 0.0016979863112773115 44-45 0.009351228960657657 46-47 0.013903800954662043 48-49 0.0050939589338319345 50-51 0.009708052170998542 52-53 0.015540266022777135 54-55 0.009326620463392768 56-57 0.0027438474450350754 58-59 0.008133108346045673 60-61 0.001513422581790647 62-63 8.489931556386558E-4 64-65 0.01914541087208331 66-67 0.04515659248107053 68-69 0.054458604447198404 70-71 0.04804809090969493 72-73 0.03952124660741104 74-75 0.027967557141545862 76-77 0.028631986567697856 78-79 0.012538029356460726 80-81 0.0019194627866613084 82-83 0.026712523781036543 84-85 0.04724831474858606 86-87 0.04806039515832738 88-89 0.05928186991111657 90-91 0.03419350694956267 92-93 0.026171136841209 94-95 0.007382549179466572 96-97 0.00361744909793862 98-99 0.022381428262416157 100 0.018973151391229088 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4063637.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.487029970488365 #Duplication Level Percentage of deduplicated Percentage of total 1 78.51158477390699 49.844673355715706 2 13.390666449860253 17.002672844541816 3 3.8042940349921235 7.2456998824828505 4 1.511531977566392 3.838507038444363 5 0.7740625155839983 2.4571465062956457 6 0.47701048820474035 1.8170387496534979 7 0.3112324391960024 1.383145623651736 8 0.21717141195298378 1.1030054351513883 9 0.16764815916055195 0.9579135334610847 >10 0.7829234307661689 8.409615493847799 >50 0.03314494422754389 1.4429008047133096 >100 0.01665745444340755 1.9231404324428818 >500 0.0013381151056161764 0.6113324117506909 >1k 6.043100272455748E-4 0.8796741048855163 >5k 4.316500194611224E-5 0.23340138166074528 >10k+ 8.633000389222448E-5 0.8501324013008854 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16623 0.40906705003424276 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14399 0.35433775211713053 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8517 0.20959057120505595 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4916 0.12097537255419222 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4553 0.11204248804703766 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.460849726488857E-5 0.0 0.0 0.0 6 0.0 2.460849726488857E-5 0.0 0.0 4.921699452977714E-5 7 0.0 2.460849726488857E-5 0.0 0.0 4.921699452977714E-5 8 0.0 2.460849726488857E-5 0.0 0.0 7.382549179466572E-5 9 0.0 2.460849726488857E-5 0.0 0.0 1.4765098358933144E-4 10-11 0.0 2.460849726488857E-5 0.0 1.1073823769199858E-4 1.4765098358933144E-4 12-13 0.0 2.460849726488857E-5 0.0 1.8456372948666427E-4 1.4765098358933144E-4 14-15 0.0 2.460849726488857E-5 0.0 2.3378072401644142E-4 1.5995523222177573E-4 16-17 0.0 2.460849726488857E-5 0.0 2.5838922128133E-4 1.7225948085422E-4 18-19 0.0 2.460849726488857E-5 0.0 4.552571994004386E-4 1.7225948085422E-4 20-21 0.0 2.460849726488857E-5 0.0 0.0010704696310226529 1.7225948085422E-4 22-23 0.0 2.460849726488857E-5 0.0 0.003715883086998174 1.7225948085422E-4 24-25 0.0 3.691274589733285E-5 0.0 0.00967113942510121 1.7225948085422E-4 26-27 0.0 4.921699452977714E-5 0.0 0.021372479874555726 1.7225948085422E-4 28-29 0.0 4.921699452977714E-5 0.0 0.057719230334796146 1.9686797811910856E-4 30-31 0.0 4.921699452977714E-5 0.0 0.16432324048629343 1.9686797811910856E-4 32-33 0.0 4.921699452977714E-5 0.0 0.39571694026804066 2.2147647538399713E-4 34-35 0.0 4.921699452977714E-5 0.0 0.7444562592574091 2.2147647538399713E-4 36-37 0.0 6.152124316222143E-5 0.0 1.2551317944983766 2.2147647538399713E-4 38-39 0.0 8.612974042711E-5 0.0 1.9686305641965558 2.2147647538399713E-4 40-41 0.0 1.1073823769199858E-4 0.0 2.7542322308808584 2.2147647538399713E-4 42-43 0.0 1.2304248632444287E-4 0.0 3.4700811120678345 2.2147647538399713E-4 44-45 0.0 1.2304248632444287E-4 0.0 4.2906760618628095 2.4608497264888573E-4 46-47 0.0 1.2304248632444287E-4 0.0 5.210541689624344 2.5838922128133E-4 48-49 0.0 1.2304248632444287E-4 0.0 6.150450938408131 2.829977185462186E-4 50-51 0.0 1.2304248632444287E-4 0.0 7.076640949966741 2.953019671786629E-4 52-53 0.0 1.4765098358933144E-4 0.0 7.960184435765301 3.568232103408843E-4 54-55 0.0 1.4765098358933144E-4 0.0 8.916741332948785 3.937359562382171E-4 56-57 0.0 1.4765098358933144E-4 0.0 9.957065554821948 4.0604020487066143E-4 58-59 0.0 1.4765098358933144E-4 0.0 11.067388647165089 4.4295295076799427E-4 60-61 0.0 1.4765098358933144E-4 0.0 12.143579753801827 4.4295295076799427E-4 62-63 0.0 1.4765098358933144E-4 0.0 13.206211578445615 4.4295295076799427E-4 64-65 0.0 1.4765098358933144E-4 0.0 14.23164765947352 4.4295295076799427E-4 66-67 0.0 1.7225948085422E-4 0.0 15.339731378565556 4.4295295076799427E-4 68-69 0.0 1.7225948085422E-4 0.0 16.536479513302986 4.552571994004386E-4 70-71 0.0 1.7225948085422E-4 0.0 17.735614672275105 4.6756144803288284E-4 72-73 0.0 1.7225948085422E-4 0.0 18.83207087640948 4.921699452977715E-4 74-75 0.0 1.7225948085422E-4 0.0 19.877304493486992 4.921699452977715E-4 76-77 0.0 1.7225948085422E-4 0.0 20.996683512823612 4.921699452977715E-4 78-79 0.0 1.7225948085422E-4 0.0 22.180413752507913 4.921699452977715E-4 80-81 0.0 1.7225948085422E-4 0.0 23.31829836178773 5.1677844256266E-4 82-83 0.0 1.7225948085422E-4 0.0 24.432177381985646 5.413869398275486E-4 84-85 0.0 1.7225948085422E-4 0.0 25.504258869579147 5.413869398275486E-4 86-87 0.0 1.7225948085422E-4 0.0 26.586749751515697 5.413869398275486E-4 88 0.0 1.7225948085422E-4 0.0 27.422011365680547 5.413869398275486E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7610 0.0 54.744873 1 TACCTGG 3850 0.0 48.341526 2 GTATCAA 13465 0.0 40.264038 1 ACCTGGG 4540 0.0 39.85573 3 ATCAACG 16810 0.0 31.649334 3 TATCAAC 17260 0.0 31.259855 2 CAACGCA 17115 0.0 31.105894 5 CCTGGGG 4525 0.0 30.95163 4 TCAACGC 17565 0.0 30.422731 4 AACGCAG 18295 0.0 29.406364 6 GTACATG 14170 0.0 29.102087 1 TGGGGGG 9640 0.0 28.074608 6 TACATGG 14360 0.0 28.048668 2 CTGGGGG 3295 0.0 27.807943 5 ACATGGG 14515 0.0 26.55111 3 ACGCAGA 20205 0.0 26.487022 7 ATGGGGG 9175 0.0 26.477327 5 CATGGGG 11580 0.0 26.21862 4 GTACCTG 7180 0.0 26.130205 1 CGCAGAG 20490 0.0 25.981022 8 >>END_MODULE