FastQCFastQC Report
Mon 6 Jun 2016
ERR1211453_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211453_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4921448
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224900.4569793280351636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187670.3813308603484178No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107910.21926473671976215No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70500.1432505230168032No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67100.1363419871549999No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93900.054.7969251
TACCTGG43300.045.8755572
GTATCAA170700.041.9022521
ACCTGGG54150.035.592543
ATCAACG208900.033.7990953
CAACGCA209900.033.5040365
TATCAAC212200.033.4956472
TCAACGC222400.031.768584
AACGCAG226000.031.451016
GTACTAG16650.029.7148951
GTACATG185050.029.078781
GTGGTAT36400.028.608171
ACGCAGA249750.028.064897
TACATGG188200.028.0377272
CGCAGAG253350.027.6079128
ACATGGG184950.027.0179563
CATGGGG135250.026.275934
CCTGGGG56000.025.6026764
ATGGGGG98650.025.3985025
GCAGAGT268350.025.364049
Produced by FastQC (version 0.11.5)