FastQCFastQC Report
Mon 6 Jun 2016
ERR1211453_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211453_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4921448
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202410.4112813952316473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182030.36987081850707354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107240.21790334877052447No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69000.14020263954836057No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59110.12010692787976221No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95700.053.9471441
TACCTGG44550.046.414952
GTATCAA171300.039.4711341
ACCTGGG53250.037.5968743
ATCAACG208350.032.2104263
CAACGCA210350.031.7652745
GTGGTAT35100.031.6142541
TATCAAC214950.031.4831332
TCAACGC220550.030.4079744
AACGCAG226900.029.963626
CCTGGGG51950.028.1349034
GTACATG189550.027.856981
TACATGG192400.027.1370052
ACGCAGA252450.026.5029897
CGCAGAG254800.026.2032348
ACATGGG191750.026.0040953
TGGGGGG104350.025.9802676
TGGTATC43100.025.5147612
CTGGGGG36500.024.9749375
CATGGGG138800.024.9544834
Produced by FastQC (version 0.11.5)