##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211453_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4921448 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03392619407946 33.0 31.0 34.0 30.0 34.0 2 32.16437865441228 33.0 31.0 34.0 30.0 34.0 3 32.2400322019048 34.0 31.0 34.0 30.0 34.0 4 35.8973489103207 37.0 35.0 37.0 35.0 37.0 5 35.753613570640184 37.0 35.0 37.0 35.0 37.0 6 35.79436092792203 37.0 35.0 37.0 35.0 37.0 7 35.72588108215306 37.0 35.0 37.0 33.0 37.0 8 35.71243483625145 37.0 35.0 37.0 33.0 37.0 9 37.288452301030105 39.0 37.0 39.0 34.0 39.0 10-11 37.26638410077685 39.0 37.0 39.0 34.0 39.0 12-13 37.178910048424775 39.0 37.0 39.0 33.0 39.0 14-15 38.3684012306947 40.0 38.0 41.0 33.0 41.0 16-17 38.35169161596343 40.0 38.0 41.0 33.0 41.0 18-19 38.36217145848133 40.0 38.0 41.0 33.5 41.0 20-21 38.3244340893168 40.0 38.0 41.0 34.0 41.0 22-23 38.21648852126447 40.0 37.5 41.0 33.5 41.0 24-25 38.09033276385324 40.0 37.0 41.0 33.0 41.0 26-27 37.95429028204707 40.0 37.0 41.0 33.0 41.0 28-29 37.869682865693186 40.0 37.0 41.0 33.0 41.0 30-31 37.89980865387585 40.0 37.0 41.0 33.0 41.0 32-33 37.719768856645445 40.0 37.0 41.0 33.0 41.0 34-35 37.597009863763674 40.0 36.0 41.0 33.0 41.0 36-37 37.44075432677538 40.0 36.0 41.0 32.0 41.0 38-39 37.315234967432346 40.0 36.0 41.0 32.0 41.0 40-41 37.23351328714638 39.0 36.0 41.0 31.0 41.0 42-43 37.08275714789632 39.0 36.0 41.0 31.0 41.0 44-45 36.908058664848234 39.0 35.0 41.0 31.0 41.0 46-47 36.6657256157131 39.0 35.0 41.0 30.0 41.0 48-49 36.4208364083091 39.0 35.0 41.0 30.0 41.0 50-51 36.22723505358586 39.0 35.0 40.0 29.0 41.0 52-53 36.009386566717765 38.5 35.0 40.0 28.5 41.0 54-55 35.79694085968195 38.0 35.0 40.0 28.0 41.0 56-57 35.55513346884901 38.0 34.5 40.0 28.0 41.0 58-59 35.277899918885666 38.0 34.0 40.0 27.0 41.0 60-61 35.011328678063855 37.0 34.0 40.0 26.5 41.0 62-63 34.70326985066184 37.0 34.0 40.0 26.0 41.0 64-65 34.35214788411866 36.0 33.0 39.0 26.0 41.0 66-67 33.99376301446241 36.0 33.0 39.0 26.0 40.5 68-69 33.6179450641356 35.0 33.0 38.5 25.0 40.0 70-71 33.24469434605425 35.0 32.0 38.0 24.5 40.0 72-73 32.891263302995384 35.0 32.0 37.0 24.0 39.5 74-75 32.52374677127544 35.0 31.5 37.0 23.5 39.0 76-77 31.505363462135534 34.0 30.5 35.5 22.5 38.0 78-79 31.996933930826863 34.5 31.0 36.0 23.0 38.0 80-81 31.894357311100308 34.5 31.0 35.0 23.0 37.0 82-83 31.634889772278402 34.0 31.0 35.0 23.0 37.0 84-85 31.375964451925533 34.0 31.0 35.0 22.0 36.0 86-87 31.14048649909539 34.0 31.0 35.0 20.5 36.0 88-89 30.919117910013476 34.0 30.5 35.0 20.0 35.5 90-91 30.702847414013114 34.0 30.0 35.0 20.0 35.0 92-93 30.53005985230363 34.0 30.0 35.0 19.5 35.0 94-95 30.376632751174043 34.0 30.0 35.0 19.0 35.0 96-97 30.219954879133134 34.0 29.0 35.0 19.0 35.0 98-99 30.02199444147332 34.0 29.0 35.0 18.0 35.0 100 28.127134331196835 32.0 25.0 34.0 12.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3449329473191085 1101 2 -0.26262887317249195 1101 3 -0.3336763662601676 1101 4 -0.16665525997794362 1101 5 -0.10707722371498107 1101 6 -0.24688064976954394 1101 7 -0.2703966605975836 1101 8 -0.32325130618954745 1101 9 -0.4306429741568607 1101 10-11 -0.4416233280423114 1101 12-13 -0.4987198217878799 1101 14-15 -0.627041950539585 1101 16-17 -0.629781830074954 1101 18-19 -0.6552461075935696 1101 20-21 -0.7502965387979401 1101 22-23 -0.7087064152726867 1101 24-25 -0.8129291231684235 1101 26-27 -0.7237131755833772 1101 28-29 -0.7663990687696227 1101 30-31 -0.7076713753750283 1101 32-33 -0.5066758467788901 1101 34-35 -0.5421572344615413 1101 36-37 -0.6180327661469391 1101 38-39 -0.5942255943352777 1101 40-41 -0.6344327017471798 1101 42-43 -0.6494521163245253 1101 44-45 -0.6298244775233073 1101 46-47 -0.7350151702306889 1101 48-49 -0.6588591232267262 1101 50-51 -0.7052413894669058 1101 52-53 -0.6815892713319016 1101 54-55 -0.6741362363794323 1101 56-57 -0.6027267438431281 1101 58-59 -0.5398313746541916 1101 60-61 -0.5952404745953928 1101 62-63 -0.7806553946421744 1101 64-65 -0.7847573425827576 1101 66-67 -0.8614161869598718 1101 68-69 -0.7623885557597134 1101 70-71 -0.8443745750657357 1101 72-73 -0.8747034489628263 1101 74-75 -0.8255086528210285 1101 76-77 -0.6750409020716965 1101 78-79 -0.6492270834093539 1101 80-81 -0.5392635786336406 1101 82-83 -0.6878531855228616 1101 84-85 -0.7650486359104463 1101 86-87 -0.8389549502386728 1101 88-89 -0.7190473821158783 1101 90-91 -0.8453709098595183 1101 92-93 -0.9936879821382156 1101 94-95 -0.9815915816655831 1101 96-97 -1.1593157165021388 1101 98-99 -1.2044460116946354 1101 100 -1.0323310841981304 1102 1 -0.3029706080918828 1102 2 -0.23176226837509262 1102 3 -0.2912069207472676 1102 4 -0.14804720973624796 1102 5 -0.18953491239376064 1102 6 -0.2070547663703195 1102 7 -0.23060785065231926 1102 8 -0.21601343851657617 1102 9 -0.4239706936594203 1102 10-11 -0.4399317798067628 1102 12-13 -0.45007364602184197 1102 14-15 -0.5212822103654204 1102 16-17 -0.5694866678626411 1102 18-19 -0.6426258384852659 1102 20-21 -0.7306819625825511 1102 22-23 -0.6405095614023324 1102 24-25 -0.779016743270212 1102 26-27 -0.7418140411047318 1102 28-29 -0.7382814422822079 1102 30-31 -0.6273578291218982 1102 32-33 -0.5667089064693513 1102 34-35 -0.6940663134105307 1102 36-37 -0.6116977285850922 1102 38-39 -0.713644993718134 1102 40-41 -0.6756777820632394 1102 42-43 -0.6671615405533871 1102 44-45 -0.527663999770418 1102 46-47 -0.6471431885797969 1102 48-49 -0.7246460457200996 1102 50-51 -0.7864552088609358 1102 52-53 -0.7638401016413212 1102 54-55 -0.7922700915229441 1102 56-57 -0.8746143045018897 1102 58-59 -0.9303639492000713 1102 60-61 -1.0182231714888914 1102 62-63 -1.0423987118514333 1102 64-65 -1.0088453774470665 1102 66-67 -0.8974619114441822 1102 68-69 -0.9800376009797063 1102 70-71 -1.1565821575439763 1102 72-73 -1.0429520121965439 1102 74-75 -0.9472743469187037 1102 76-77 -0.7581403432194485 1102 78-79 -0.8282913170116437 1102 80-81 -0.6342930899396322 1102 82-83 -0.7006761298181416 1102 84-85 -0.7737376024949754 1102 86-87 -0.9461923211587404 1102 88-89 -0.9190707974793249 1102 90-91 -0.7627601428500199 1102 92-93 -0.8586303828540451 1102 94-95 -0.9320719835606468 1102 96-97 -1.0752466398204312 1102 98-99 -1.1274702005285064 1102 100 -1.0273324426866104 1103 1 -0.2992043154253068 1103 2 -0.2822994650927555 1103 3 -0.2988451425613441 1103 4 -0.1435344192711412 1103 5 -0.16033205936368233 1103 6 -0.32570707023153744 1103 7 -0.3319083061568264 1103 8 -0.41084795544799135 1103 9 -0.5487330115773403 1103 10-11 -0.4591410696197471 1103 12-13 -0.5342539491844605 1103 14-15 -0.7126419913176036 1103 16-17 -0.7956131752952231 1103 18-19 -0.787610852189836 1103 20-21 -0.6971353374568423 1103 22-23 -0.7626999831313341 1103 24-25 -0.8243200044796524 1103 26-27 -0.916037119999686 1103 28-29 -0.835376157359498 1103 30-31 -0.7564794124859802 1103 32-33 -0.756161596819247 1103 34-35 -0.7478021827706058 1103 36-37 -0.7213494521499157 1103 38-39 -0.7376596870483851 1103 40-41 -0.7394547510568188 1103 42-43 -0.8534539395156244 1103 44-45 -0.8647577570769371 1103 46-47 -0.8217046563353136 1103 48-49 -0.8806992977743775 1103 50-51 -0.8733679756634771 1103 52-53 -0.8815367474717775 1103 54-55 -0.906535664452953 1103 56-57 -0.9755091159196425 1103 58-59 -0.9462203371084925 1103 60-61 -0.9114472250564418 1103 62-63 -1.030605807242587 1103 64-65 -1.0102084401726046 1103 66-67 -0.9935766721000903 1103 68-69 -1.0253450876483896 1103 70-71 -0.9265474524387756 1103 72-73 -1.0955607403051424 1103 74-75 -1.083982742342812 1103 76-77 -1.0805175302467234 1103 78-79 -0.8996251677289884 1103 80-81 -0.7773636184882413 1103 82-83 -0.7922619024151949 1103 84-85 -0.8641423592352879 1103 86-87 -0.8788149087736379 1103 88-89 -0.803336173221247 1103 90-91 -1.0678238627753132 1103 92-93 -0.9787630059346277 1103 94-95 -1.035329178813484 1103 96-97 -1.1759340516139716 1103 98-99 -1.1735963699243541 1103 100 -1.3885592061820233 1104 1 -0.3838604216861583 1104 2 -0.38510987377654615 1104 3 -0.3733687764678635 1104 4 -0.20145784444144255 1104 5 -0.17634368945290646 1104 6 -0.32144049814189657 1104 7 -0.31187303386190734 1104 8 -0.3265125208763351 1104 9 -0.47859913817355704 1104 10-11 -0.46809267258841913 1104 12-13 -0.5648177209835197 1104 14-15 -0.694828989207501 1104 16-17 -0.6638925990704152 1104 18-19 -0.7736929213394248 1104 20-21 -0.743869086569191 1104 22-23 -0.787423017344139 1104 24-25 -0.7862375766359477 1104 26-27 -0.7291264377506508 1104 28-29 -0.7995460411280746 1104 30-31 -0.7477436941620468 1104 32-33 -0.6603728481372357 1104 34-35 -0.7595062751872632 1104 36-37 -0.7542925421257678 1104 38-39 -0.8501134734958882 1104 40-41 -0.9844692926455849 1104 42-43 -0.8330381513110225 1104 44-45 -0.8287134266420182 1104 46-47 -0.8215839072967341 1104 48-49 -0.7855418963382377 1104 50-51 -0.7097152711103902 1104 52-53 -0.7906899859562202 1104 54-55 -0.7385490398616099 1104 56-57 -0.8039835952629986 1104 58-59 -0.8754962676463478 1104 60-61 -0.9544218980384116 1104 62-63 -0.9511358377193915 1104 64-65 -1.0492570459295436 1104 66-67 -0.9977363889799165 1104 68-69 -0.96255267094255 1104 70-71 -0.9488185175380934 1104 72-73 -0.9350218442928693 1104 74-75 -0.886520015322553 1104 76-77 -0.8259732008989218 1104 78-79 -0.7989031552959176 1104 80-81 -0.8963044972241256 1104 82-83 -0.8563091386754564 1104 84-85 -0.9265631955663558 1104 86-87 -0.860177159442383 1104 88-89 -0.7816143120824286 1104 90-91 -0.8226554019575261 1104 92-93 -1.0172652713446233 1104 94-95 -0.938508786830397 1104 96-97 -1.174870802503932 1104 98-99 -1.1583732411327503 1104 100 -1.2933098509325731 1105 1 -0.40246550768546285 1105 2 -0.3653425756680555 1105 3 -0.39255342886709954 1105 4 -0.20779435631190069 1105 5 -0.21509836224171153 1105 6 -0.2550597259954941 1105 7 -0.3333238560046894 1105 8 -0.26571251732251255 1105 9 -0.40693740100997644 1105 10-11 -0.43591134084569916 1105 12-13 -0.5251509416275937 1105 14-15 -0.8011397459366592 1105 16-17 -0.669677712649225 1105 18-19 -0.6892547259297785 1105 20-21 -0.7116909855772349 1105 22-23 -0.7329885014567452 1105 24-25 -0.7456663889862227 1105 26-27 -0.7518300592253908 1105 28-29 -0.793441234664428 1105 30-31 -0.7085708663106303 1105 32-33 -0.5905911807598869 1105 34-35 -0.686329997398829 1105 36-37 -0.8132789082060725 1105 38-39 -0.7561137954604007 1105 40-41 -0.7379340835450847 1105 42-43 -0.7624793369319391 1105 44-45 -0.7268755938740625 1105 46-47 -0.8179496660433472 1105 48-49 -0.7897824838866043 1105 50-51 -0.7721538458303527 1105 52-53 -0.8331628995184417 1105 54-55 -0.8852246452752794 1105 56-57 -0.7956008066233053 1105 58-59 -0.715127453215274 1105 60-61 -0.92015887746917 1105 62-63 -0.8835508789440212 1105 64-65 -0.9428202899203271 1105 66-67 -0.9072226153050167 1105 68-69 -0.920953776424696 1105 70-71 -0.9286917226856772 1105 72-73 -0.9396983307928473 1105 74-75 -0.8886377219721027 1105 76-77 -0.8670511873355231 1105 78-79 -0.8205425472746803 1105 80-81 -0.6481805664322593 1105 82-83 -0.7539379796279668 1105 84-85 -0.5102250011814675 1105 86-87 -0.5170070650080447 1105 88-89 -0.7035872639340965 1105 90-91 -0.6500515829173317 1105 92-93 -0.5873686508260327 1105 94-95 -0.6908466514767788 1105 96-97 -0.7711451562439855 1105 98-99 -0.8531354924551735 1105 100 -0.9442280378736037 1106 1 -0.38488260094163707 1106 2 -0.4025115051138606 1106 3 -0.42504842107720364 1106 4 -0.24251503291996102 1106 5 -0.305461148798571 1106 6 -0.34631278631154316 1106 7 -0.3700476485709032 1106 8 -0.3478402716069553 1106 9 -0.5010838510433615 1106 10-11 -0.5587691196126698 1106 12-13 -0.6727138172118359 1106 14-15 -0.7200135496312967 1106 16-17 -0.7248746857184472 1106 18-19 -0.8059918726023838 1106 20-21 -0.676080035209992 1106 22-23 -0.6939277402726844 1106 24-25 -0.8295746907890234 1106 26-27 -0.8509842991541703 1106 28-29 -0.8567619557861761 1106 30-31 -0.7860246833593649 1106 32-33 -0.7197921600610258 1106 34-35 -0.7587760245521764 1106 36-37 -0.7975878332005095 1106 38-39 -0.8510392350330633 1106 40-41 -0.8329708073376523 1106 42-43 -0.7791719699165824 1106 44-45 -0.890574681343459 1106 46-47 -0.8714773758141021 1106 48-49 -0.851100089050199 1106 50-51 -0.852834905256131 1106 52-53 -0.8152461401149296 1106 54-55 -0.8137801761010124 1106 56-57 -0.8288747883726941 1106 58-59 -0.8757105825275389 1106 60-61 -0.8661860308188309 1106 62-63 -0.8493866234354499 1106 64-65 -0.9515004590723422 1106 66-67 -0.8819610973882561 1106 68-69 -0.8076664405689229 1106 70-71 -0.7382844224408487 1106 72-73 -0.8405440908640678 1106 74-75 -0.8652680357936013 1106 76-77 -0.787149117449033 1106 78-79 -0.7578895314723191 1106 80-81 -0.7247441097218186 1106 82-83 -0.6694732567256771 1106 84-85 -0.6780416047870794 1106 86-87 -0.706078954614096 1106 88-89 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1.1329264331716722 2210 80-81 1.04721286829475 2210 82-83 1.1110100374215754 2210 84-85 1.1029045567313744 2210 86-87 1.1629045262305482 2210 88-89 1.1089960503376552 2210 90-91 1.2559978225898298 2210 92-93 1.2363003408912867 2210 94-95 1.315149554744771 2210 96-97 1.3635627028589106 2210 98-99 1.3855459807081054 2210 100 1.4191119524652294 2211 1 0.6410480640948357 2211 2 0.6062602563009705 2211 3 0.5289100758656886 2211 4 0.3790936914593388 2211 5 0.4613916188775349 2211 6 0.4902781936793801 2211 7 0.4446444712845903 2211 8 0.5004587341047753 2211 9 0.7264531844436704 2211 10-11 0.677094654235944 2211 12-13 0.7134488380501338 2211 14-15 0.8636542940250393 2211 16-17 0.8853782295828339 2211 18-19 0.8011894578439112 2211 20-21 0.937570027254111 2211 22-23 0.9079578006630982 2211 24-25 0.9286584064355949 2211 26-27 0.9653675795002741 2211 28-29 1.035313238046804 2211 30-31 0.9936750184687284 2211 32-33 1.0288862781717611 2211 34-35 0.9574429732311671 2211 36-37 1.0998020642867274 2211 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1.0783872204058866 2311 50-51 1.113533319622526 2311 52-53 1.1102900545191687 2311 54-55 1.0353295902319104 2311 56-57 1.03285988538088 2311 58-59 1.061723745177737 2311 60-61 1.113457357189823 2311 62-63 1.0886367527036427 2311 64-65 1.1219045711576712 2311 66-67 1.2191294192374187 2311 68-69 1.171133256884623 2311 70-71 1.2279416854824277 2311 72-73 1.1908343744919776 2311 74-75 1.039622883715701 2311 76-77 0.9206467001199918 2311 78-79 1.020875988418954 2311 80-81 1.0166667015601902 2311 82-83 1.044478664902126 2311 84-85 1.0296341846655395 2311 86-87 1.0818569081032194 2311 88-89 1.0643391080281326 2311 90-91 1.1239717286162083 2311 92-93 1.211541909307595 2311 94-95 1.242032029784788 2311 96-97 1.2536676355954945 2311 98-99 1.3114189122788709 2311 100 1.4824478797591958 2312 1 0.25462676941736717 2312 2 0.2019519313314646 2312 3 0.3431856169423426 2312 4 0.25518112757522005 2312 5 0.35400659575964966 2312 6 0.4221194951060454 2312 7 0.4579658816169072 2312 8 0.3408969494448755 2312 9 0.4648939919174353 2312 10-11 0.30051872968743254 2312 12-13 0.4990122114746356 2312 14-15 0.8668351222347113 2312 16-17 0.8866836557690831 2312 18-19 0.9259264149905775 2312 20-21 0.896765343857453 2312 22-23 0.47684879589070306 2312 24-25 0.49377890519024703 2312 26-27 0.7620140392671217 2312 28-29 1.0068934338898003 2312 30-31 0.8433437061160447 2312 32-33 0.7168939685364748 2312 34-35 0.8807104248626416 2312 36-37 0.9701269733309772 2312 38-39 0.9236977670554367 2312 40-41 0.9596341522765144 2312 42-43 0.8739285526543767 2312 44-45 0.8003279997049688 2312 46-47 0.9246559102352947 2312 48-49 1.0089591600725711 2312 50-51 1.0207536778367512 2312 52-53 0.9615861360657547 2312 54-55 0.9736304573576078 2312 56-57 1.015142361350975 2312 58-59 0.9972475987031331 2312 60-61 1.0591089387739174 2312 62-63 1.0984395773668183 2312 64-65 1.110274521388881 2312 66-67 1.2046095062351867 2312 68-69 1.2204822434975497 2312 70-71 1.0321615246409905 2312 72-73 1.0574705910632431 2312 74-75 1.1029785632069462 2312 76-77 1.0258144410958643 2312 78-79 0.9470905369831293 2312 80-81 0.9130835502034671 2312 82-83 0.9904989383474962 2312 84-85 0.7649736264459719 2312 86-87 1.0274834363667367 2312 88-89 0.6923514407555338 2312 90-91 0.9716023908970115 2312 92-93 1.0934665747185228 2312 94-95 1.1348524325772473 2312 96-97 1.2660126922155612 2312 98-99 1.257087210702089 2312 100 1.3442620999537525 2313 1 -0.39076784893211425 2313 2 -0.0778578737908262 2313 3 0.17055014511736033 2313 4 0.07623944680003092 2313 5 0.18606836106589242 2313 6 0.30931376044230774 2313 7 0.12236077613319907 2313 8 0.036870917721714136 2313 9 0.26324659689083774 2313 10-11 0.35412991084085377 2313 12-13 0.5656224440551156 2313 14-15 0.5960835883082112 2313 16-17 0.699724732579476 2313 18-19 0.5947435805585783 2313 20-21 0.7986288694268993 2313 22-23 0.8490976341890359 2313 24-25 0.6487144862812286 2313 26-27 0.871909215953842 2313 28-29 0.7567355385209922 2313 30-31 0.7217846844669751 2313 32-33 0.8403357273588057 2313 34-35 0.8695640924778871 2313 36-37 0.7642602298178929 2313 38-39 0.4886341886540819 2313 40-41 0.6051272300955262 2313 42-43 0.7311010848638304 2313 44-45 0.971529805595722 2313 46-47 0.9667113116977788 2313 48-49 0.9215967932029869 2313 50-51 0.8415346295315729 2313 52-53 0.8222683881460142 2313 54-55 0.9022113201987665 2313 56-57 0.9495719568266381 2313 58-59 0.8729893467189314 2313 60-61 0.8699923123315401 2313 62-63 1.0132048956190474 2313 64-65 1.0075332656704248 2313 66-67 1.0257674856547538 2313 68-69 0.9827182340358362 2313 70-71 0.9533231062362759 2313 72-73 1.0294756821382478 2313 74-75 0.7620830623244146 2313 76-77 0.8819723604791712 2313 78-79 0.816356773790158 2313 80-81 0.8824674580838305 2313 82-83 0.8882751952066421 2313 84-85 0.9500510131875615 2313 86-87 0.9929289654663194 2313 88-89 0.9031231468660224 2313 90-91 1.0683604327542895 2313 92-93 0.8187202279250592 2313 94-95 0.7939821469247121 2313 96-97 1.1672650486388392 2313 98-99 1.2539761316103224 2313 100 1.3094722869066722 2314 1 -0.40338847656172305 2314 2 -0.18200205221593535 2314 3 0.11311005739090518 2314 4 -0.08460907718402666 2314 5 0.15907268564997423 2314 6 0.16965200503814515 2314 7 0.07884331145622525 2314 8 0.12996386282058126 2314 9 0.20441230065348748 2314 10-11 0.41678717974968293 2314 12-13 0.5439116717915979 2314 14-15 0.6218162060519745 2314 16-17 0.7975682055594504 2314 18-19 0.8369650737864518 2314 20-21 0.4203051333329739 2314 22-23 0.641099545505007 2314 24-25 0.877834674447179 2314 26-27 0.839095720405787 2314 28-29 0.798234482349919 2314 30-31 0.749249997911754 2314 32-33 0.807687536655699 2314 34-35 0.7913387433635677 2314 36-37 0.6559723227211478 2314 38-39 0.7684126864311409 2314 40-41 0.7413473356294915 2314 42-43 0.5306762313778464 2314 44-45 0.6266282041598927 2314 46-47 0.7166680740342812 2314 48-49 0.5872570387983629 2314 50-51 0.6796482244478881 2314 52-53 0.757353165994374 2314 54-55 0.7165940819385952 2314 56-57 0.6980308765487848 2314 58-59 0.6641933135990925 2314 60-61 0.7977421970651832 2314 62-63 0.5449316095988692 2314 64-65 0.6713756077248476 2314 66-67 0.7574726114678398 2314 68-69 0.8211394330704138 2314 70-71 0.8112582907639947 2314 72-73 0.8556977134348216 2314 74-75 0.8704305140553075 2314 76-77 0.6440898843327965 2314 78-79 0.7580178878186139 2314 80-81 0.7156358500093631 2314 82-83 0.741144901761583 2314 84-85 0.6780113689180283 2314 86-87 0.7799884404568189 2314 88-89 0.7661499086968924 2314 90-91 0.9402127887742324 2314 92-93 0.9466456391735072 2314 94-95 1.0320098934431101 2314 96-97 1.0950155330437745 2314 98-99 1.033390465362558 2314 100 1.0803674039877222 2315 1 -0.07109960710831231 2315 2 0.13611474161211845 2315 3 0.17596711112800278 2315 4 0.006480807325424109 2315 5 0.28890241491761515 2315 6 0.4032510663919311 2315 7 0.4475944781728174 2315 8 0.4165882604181803 2315 9 0.6413205110970992 2315 10-11 0.6112416958785261 2315 12-13 0.6831995305282774 2315 14-15 0.7209959091326468 2315 16-17 0.7599307836515479 2315 18-19 0.8487326323838076 2315 20-21 0.8526524665934829 2315 22-23 0.9409550861641947 2315 24-25 0.8760044777237042 2315 26-27 0.9489407051145378 2315 28-29 1.0093865795433885 2315 30-31 0.9539132278692009 2315 32-33 0.9851947366796381 2315 34-35 1.0010948785754294 2315 36-37 0.9711211937691715 2315 38-39 0.9879862030108413 2315 40-41 0.9443019010130698 2315 42-43 0.9885971701259066 2315 44-45 0.8930005501266436 2315 46-47 0.8824252874925307 2315 48-49 0.9706668208579643 2315 50-51 0.8337605785065278 2315 52-53 0.7927765119118391 2315 54-55 0.7270137264567964 2315 56-57 0.9006572229045915 2315 58-59 0.932950681585119 2315 60-61 0.9426565142473251 2315 62-63 0.994971180488001 2315 64-65 1.0351273656574094 2315 66-67 1.0426679055690116 2315 68-69 0.8898443528520374 2315 70-71 0.5505325613469623 2315 72-73 0.864167286818784 2315 74-75 0.97801074901286 2315 76-77 0.8778163528626273 2315 78-79 0.8274667212187161 2315 80-81 0.8865780063822513 2315 82-83 0.8412333905344944 2315 84-85 0.7391797305187708 2315 86-87 0.7130898724832413 2315 88-89 0.756530872465401 2315 90-91 0.8872088572542864 2315 92-93 0.8952845401027325 2315 94-95 0.9353885190306706 2315 96-97 1.0711149706379715 2315 98-99 0.9788650080905512 2315 100 0.9363497113257111 2316 1 0.4105609196843112 2316 2 0.4622401233644595 2316 3 0.4763872607546489 2316 4 0.26561982191795863 2316 5 0.34438370239141847 2316 6 0.4076039782847616 2316 7 0.42183145940710176 2316 8 0.46869258233636657 2316 9 0.4642476165423517 2316 10-11 0.4372284889666176 2316 12-13 0.6308612406408116 2316 14-15 0.7425265267516536 2316 16-17 0.7450938287086117 2316 18-19 0.7651120726287601 2316 20-21 0.7965715251707479 2316 22-23 0.8147173235040555 2316 24-25 0.8921859647651829 2316 26-27 0.9661072297006115 2316 28-29 0.905005124394286 2316 30-31 0.7207655833174371 2316 32-33 0.6994109345778767 2316 34-35 0.6383628098325218 2316 36-37 0.6806654776673042 2316 38-39 0.6775959509168814 2316 40-41 0.7784106690541606 2316 42-43 0.928400001692161 2316 44-45 0.86201768924699 2316 46-47 0.8129993157312825 2316 48-49 0.6857669558049153 2316 50-51 0.6662812066650687 2316 52-53 0.8869947548092583 2316 54-55 0.9140230755618717 2316 56-57 0.9056871946298841 2316 58-59 0.8981722154996064 2316 60-61 1.013136726450739 2316 62-63 0.969956932371602 2316 64-65 0.9313503974266908 2316 66-67 0.8938110621992266 2316 68-69 0.8764831777831859 2316 70-71 0.790846872600234 2316 72-73 0.9577375199486866 2316 74-75 0.9153623507268591 2316 76-77 0.8223979723640333 2316 78-79 0.7833817324322574 2316 80-81 0.7505108198517831 2316 82-83 0.6586310825799835 2316 84-85 0.7459276530733874 2316 86-87 0.6585052967719776 2316 88-89 0.6545685928252993 2316 90-91 0.6822810518905555 2316 92-93 0.7833579053103286 2316 94-95 0.6555452984694128 2316 96-97 0.9268241339271306 2316 98-99 0.9397074537047558 2316 100 0.5467137295390572 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 6.0 12 40.0 13 198.0 14 769.0 15 2183.0 16 4997.0 17 9988.0 18 15737.0 19 22085.0 20 29173.0 21 37773.0 22 44226.0 23 45898.0 24 46282.0 25 49622.0 26 57092.0 27 68613.0 28 83865.0 29 102041.0 30 124359.0 31 152797.0 32 191826.0 33 247728.0 34 344754.0 35 475341.0 36 706675.0 37 1048811.0 38 883353.0 39 125200.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.470597419579384 19.721062971956265 13.068522804341143 23.73981680412321 2 16.070290694933686 21.097246176328593 39.471533581173674 23.360929547564048 3 18.55051602699043 26.820155368907688 30.741013620381647 23.88831498372024 4 12.658321227989886 17.303684115973933 37.84766451547872 32.190330140557464 5 12.634594869189739 37.89943611887224 34.523362966725934 14.94260604521209 6 29.62471614045297 37.21945248634142 18.67129348923325 14.48453788397236 7 26.37004080715384 33.409158008802706 22.617115596834342 17.60368558720911 8 26.097277843488143 34.98784600976668 20.533182537023293 18.381693609721893 9 27.255963600276022 15.584810365273311 20.641038770615253 36.518187263835415 10-11 24.986700976589248 25.964387470755355 28.570203604086142 20.47870794856925 12-13 25.3452882560694 24.22673159222068 28.300475176785454 22.12750497492447 14-15 22.89634997519022 25.640980313509488 26.309076641296077 25.15359307000422 16-17 21.915970580173337 28.151885534384935 27.219567513024128 22.7125763724176 18-19 21.517244518279984 27.380102360118403 29.503064951615865 21.599588169985743 20-21 22.396317343627054 26.823592042788224 29.17844800742058 21.60164260616414 22-23 22.35623767194509 26.604784466358726 29.02120768330862 22.017770178387558 24-25 21.977387951145264 26.54584109104497 29.364290624281843 22.112480333527923 26-27 21.89228657907185 26.70274073809172 29.111584639317535 22.2933880435189 28-29 21.58578940229884 27.503021245489723 29.01576229109719 21.895427061114248 30-31 22.4837558843804 26.804114075321085 29.149707984368263 21.562422055930252 32-33 22.371463763630338 26.95086280844744 28.753805581404237 21.92386784651799 34-35 22.375896964327108 26.904951378240632 29.20108250013998 21.518069157292278 36-37 22.160057358976847 26.686365606565595 29.09213971646738 22.06143731799018 38-39 22.12220596910123 26.72616020270617 29.163786239901146 21.987847588291455 40-41 21.990020606767345 26.215337518489246 29.65737782314389 22.137264051599516 42-43 21.730733593369646 26.566933476166366 29.03979465855401 22.66253827190998 44-45 21.202038502403646 26.93302767515846 29.38322774260458 22.481706079833316 46-47 21.23416452031538 26.463281846643827 29.120110454617322 23.18244317842347 48-49 21.49311256196317 26.497705474278018 29.044579748777196 22.96460221498162 50-51 21.302839977654582 26.681103671895436 28.867514380952013 23.148541969497973 52-53 21.039977637162146 26.958874550149588 28.628273402560446 23.37287441012782 54-55 20.81686164452 26.635816891618447 28.92965546139678 23.61766600246477 56-57 21.277384762485138 26.467282962781134 28.353377062458225 23.901955212275503 58-59 20.819481180309108 26.62827147889785 28.648829835139733 23.903417505653305 60-61 21.202982020018034 26.81137863867767 28.184179501528234 23.80145983977606 62-63 21.162984819147894 26.587521795132563 28.035860549352144 24.213632836367395 64-65 20.632811063092102 26.378075014658048 28.078774434737895 24.910339487511955 66-67 20.92851882890268 26.30031209136791 27.771954263190445 24.999214816538963 68-69 20.67741880395399 26.416531843097484 27.787208763809844 25.118840589138674 70-71 20.630363231206942 26.147639870793032 27.83308560769794 25.388911290302087 72-73 20.68764696699158 26.426738479251433 27.476403971808356 25.40921058194863 74-75 20.777128223108054 26.53899487554719 27.25939588875085 25.42448101259391 76-77 20.52395706905258 26.41805489202782 27.357742278747505 25.7002457601721 78-79 20.483613799897903 26.43792289459053 27.434245073206892 25.644218232304677 80-81 20.440581447624755 26.476633638721182 27.260353511621588 25.822431402032475 82-83 20.63419056000553 26.111200313812848 27.513653094532053 25.74095603164957 84-85 20.73775610644612 26.25539067147021 27.05034573074269 25.956507491340975 86-87 20.287970222104317 26.47983313969976 27.282350274492106 25.94984636370382 88-89 20.445882495977465 25.86569423678337 27.317477266618656 26.37094600062051 90-91 20.74416788530587 25.52258863662857 27.331923577220735 26.40131990084483 92-93 20.67385213833007 25.534029943541466 27.35936710994837 26.4327508081801 94-95 20.073766709960232 25.341214977399662 27.76668090142062 26.818337411219485 96-97 20.1393550510872 25.23896150210033 27.81010080279523 26.81158264401724 98-99 20.027475908278554 25.484916043408234 27.605564730112054 26.88204331820116 100 19.980205223463518 25.49941164396229 27.597922158159044 26.922460974415152 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3517.0 1 2431.0 2 2933.0 3 4206.0 4 2814.0 5 1917.5 6 2757.0 7 4402.5 8 6217.5 9 7050.0 10 5908.0 11 4545.5 12 4460.5 13 4819.5 14 5370.0 15 5844.0 16 6357.5 17 7032.5 18 7583.5 19 8211.0 20 9013.0 21 9877.5 22 11042.0 23 12766.5 24 14902.0 25 17509.0 26 21285.0 27 25955.5 28 31204.5 29 38201.0 30 46958.0 31 55604.5 32 66411.0 33 76831.0 34 86126.0 35 95984.5 36 105747.5 37 119376.5 38 128716.5 39 130389.5 40 131303.5 41 134541.5 42 141050.0 43 149340.5 44 160472.0 45 176629.5 46 190462.5 47 201768.5 48 213886.5 49 220561.5 50 221936.0 51 221805.0 52 216187.0 53 203042.0 54 188973.0 55 170287.0 56 150023.0 57 129007.5 58 108279.5 59 92311.5 60 74406.0 61 57398.5 62 44553.0 63 33798.0 64 24493.5 65 17186.5 66 12098.0 67 9070.0 68 6830.5 69 4914.5 70 3655.5 71 2742.0 72 2181.0 73 1757.0 74 1304.5 75 936.5 76 635.5 77 399.5 78 247.0 79 146.0 80 103.0 81 75.0 82 44.0 83 26.0 84 18.0 85 10.5 86 4.5 87 6.5 88 8.0 89 4.5 90 1.5 91 2.0 92 1.5 93 1.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05201721119475406 2 0.0 3 0.0 4 0.01536133268095081 5 0.00402320617834426 6 0.0 7 1.4223456186065564E-4 8 3.860652393360653E-4 9 0.0018693685273114742 10-11 6.908535861803274E-4 12-13 3.352671815286883E-4 14-15 4.063844624590161E-5 16-17 1.0159611561475402E-4 18-19 0.0 20-21 1.0159611561475403E-5 22-23 1.0159611561475403E-5 24-25 2.0319223122950806E-5 26-27 0.0 28-29 0.0010261207677090157 30-31 0.0010261207677090157 32-33 0.014528244532909827 34-35 0.02380396988853687 36-37 0.03160655156774998 38-39 0.020837363312586052 40-41 1.3207495029918023E-4 42-43 0.0017068147423278677 44-45 0.009804025156823763 46-47 0.014101540847327858 48-49 0.005658903639741799 50-51 0.010748869032040975 52-53 0.01594043053995491 54-55 0.010251048065528682 56-57 0.003383150649971309 58-59 0.008777904389114749 60-61 0.0017474531885737692 62-63 0.0010362803792704912 64-65 0.01916102740494261 66-67 0.045017238828897514 68-69 0.05433360263077046 70-71 0.04802448385109423 72-73 0.040150784890950794 74-75 0.028660264214922113 76-77 0.02859930654555326 78-79 0.012191533873770484 80-81 0.002245274155086064 82-83 0.026780736076049163 84-85 0.046774851629032756 86-87 0.04801432423953275 88-89 0.05912893928778684 90-91 0.034227731350610635 92-93 0.026181318993922117 94-95 0.007995614298881144 96-97 0.004175600351766391 98-99 0.023113116302356542 100 0.019465815751786873 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4921448.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.73717089421602 #Duplication Level Percentage of deduplicated Percentage of total 1 76.78643892725489 48.17363941337472 2 14.363608809855993 18.022643611232045 3 4.215218812243758 7.933527089407528 4 1.7018810301071392 4.2708480410982395 5 0.8915367443026098 2.79662465428929 6 0.5046807168934294 1.8997344229655124 7 0.3313510420636307 1.4551618866345792 8 0.2247700076750795 1.1281147506724567 9 0.1653201379354848 0.9334545971322489 >10 0.770503941812882 8.284676313173076 >50 0.030378483576138416 1.3005233995560772 >100 0.012907978944339454 1.4525668505192535 >500 8.124759672462658E-4 0.3644761027614418 >1k 4.0623781511837546E-4 0.5479998800274247 >5k 7.386142093058693E-5 0.29682168370666645 >10k+ 1.107921313958804E-4 1.1391873034493307 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20241 0.4112813952316473 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18203 0.36987081850707354 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10724 0.21790334877052447 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6900 0.14020263954836057 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5911 0.12010692787976221 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.127689249180323E-5 2 0.0 0.0 0.0 0.0 8.127689249180323E-5 3 0.0 0.0 0.0 0.0 8.127689249180323E-5 4 0.0 0.0 0.0 2.0319223122950806E-5 8.127689249180323E-5 5 0.0 0.0 0.0 4.063844624590161E-5 1.0159611561475402E-4 6 4.063844624590161E-5 0.0 0.0 4.063844624590161E-5 1.0159611561475402E-4 7 4.063844624590161E-5 0.0 0.0 4.063844624590161E-5 1.0159611561475402E-4 8 4.063844624590161E-5 0.0 0.0 4.063844624590161E-5 1.0159611561475402E-4 9 4.063844624590161E-5 0.0 0.0 8.127689249180323E-5 1.0159611561475402E-4 10-11 4.063844624590161E-5 0.0 0.0 1.1175572717622943E-4 1.2191533873770484E-4 12-13 4.063844624590161E-5 0.0 0.0 2.4383067747540968E-4 2.3367106591393427E-4 14-15 4.063844624590161E-5 0.0 0.0 2.6414990059836046E-4 3.352671815286883E-4 16-17 4.063844624590161E-5 0.0 0.0 3.251075699672129E-4 4.6734213182786854E-4 18-19 4.063844624590161E-5 0.0 0.0 5.689382474426226E-4 5.486190243196717E-4 20-21 4.063844624590161E-5 0.0 0.0 0.0012394726104999992 6.095766936885242E-4 22-23 4.063844624590161E-5 0.0 0.0 0.004185759963327866 6.400555283729504E-4 24-25 4.063844624590161E-5 0.0 0.0 0.011368605337290977 6.908535861803274E-4 26-27 4.063844624590161E-5 0.0 0.0 0.023235031641094247 7.213324208647536E-4 28-29 4.063844624590161E-5 0.0 0.0 0.05772691289230324 7.518112555491798E-4 30-31 4.063844624590161E-5 0.0 0.0 0.1634478308010163 7.518112555491798E-4 32-33 4.063844624590161E-5 0.0 0.0 0.3758751489399055 7.924497017950814E-4 34-35 6.095766936885242E-5 0.0 0.0 0.690650393949098 8.330881480409831E-4 36-37 6.095766936885242E-5 0.0 0.0 1.1550665576472616 8.534073711639338E-4 38-39 6.095766936885242E-5 0.0 0.0 1.7858565202761465 8.8388620584836E-4 40-41 6.095766936885242E-5 0.0 0.0 2.4498887319341787 9.042054289713109E-4 42-43 6.095766936885242E-5 0.0 0.0 3.079144593217281 9.143650405327863E-4 44-45 6.095766936885242E-5 0.0 0.0 3.8114900330146737 9.448438752172125E-4 46-47 6.095766936885242E-5 0.0 0.0 4.64233290690057 0.001005801544586065 48-49 6.095766936885242E-5 0.0 0.0 5.489624191904497 0.0010769188255163927 50-51 1.1175572717622943E-4 0.0 0.0 6.333339293638783 0.001137876494885245 52-53 1.2191533873770484E-4 0.0 0.0 7.124884790004893 0.0012191533873770484 54-55 1.2191533873770484E-4 0.0 0.0 7.986399531194884 0.0012394726104999992 56-57 1.2191533873770484E-4 0.0 0.0 8.931527875535817 0.0012496322220614748 58-59 1.2191533873770484E-4 0.0 0.0 9.95046579787087 0.00125979183362295 60-61 1.3207495029918023E-4 0.0 0.0 10.945264483135858 0.00125979183362295 62-63 1.4223456186065564E-4 0.0 0.0 11.9276278038496 0.0013410687261147531 64-65 1.4223456186065564E-4 0.0 0.0 12.883931720908155 0.0013410687261147531 66-67 1.4223456186065564E-4 0.0 0.0 13.906527103405338 0.0013512283376762284 68-69 1.4223456186065564E-4 0.0 0.0 15.005431328340766 0.0013817071723606549 70-71 1.5239417342213104E-4 0.0 0.0 16.124268711159804 0.0014020263954836057 72-73 1.6255378498360645E-4 0.0 0.0 17.16721379561462 0.001412186007045081 74-75 1.6255378498360645E-4 0.0 0.0 18.156587248305783 0.0014426648417295072 76-77 1.6255378498360645E-4 0.0 0.0 19.195346572797277 0.0014629840648524581 78-79 1.6255378498360645E-4 0.0 0.0 20.305263816665338 0.0014833032879754088 80-81 1.6255378498360645E-4 0.0 0.0 21.392118742288854 0.0015341013457827858 82-83 1.6255378498360645E-4 0.0 0.0 22.469139163920865 0.0016052186267131137 84-85 1.7271339654508186E-4 0.0 0.0 23.495899987158253 0.0016052186267131137 86-87 1.9303261966803264E-4 0.0 0.0 24.530991691876046 0.00163569746139754 88 2.4383067747540968E-4 0.0 0.0 25.320820213888272 0.0016661762960819661 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9570 0.0 53.947144 1 TACCTGG 4455 0.0 46.41495 2 GTATCAA 17130 0.0 39.471134 1 ACCTGGG 5325 0.0 37.596874 3 ATCAACG 20835 0.0 32.210426 3 CAACGCA 21035 0.0 31.765274 5 GTGGTAT 3510 0.0 31.614254 1 TATCAAC 21495 0.0 31.483133 2 TCAACGC 22055 0.0 30.407974 4 AACGCAG 22690 0.0 29.96362 6 CCTGGGG 5195 0.0 28.134903 4 GTACATG 18955 0.0 27.85698 1 TACATGG 19240 0.0 27.137005 2 ACGCAGA 25245 0.0 26.502989 7 CGCAGAG 25480 0.0 26.203234 8 ACATGGG 19175 0.0 26.004095 3 TGGGGGG 10435 0.0 25.980267 6 TGGTATC 4310 0.0 25.514761 2 CTGGGGG 3650 0.0 24.974937 5 CATGGGG 13880 0.0 24.954483 4 >>END_MODULE