FastQCFastQC Report
Mon 6 Jun 2016
ERR1211452_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211452_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5949012
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239380.40238614411939333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198400.3335007560919359No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114630.192687458018239No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66960.11255650518102837No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107850.053.2381361
TACCTGG47800.045.776232
GTATCAA192650.040.1950041
ACCTGGG62000.034.3476943
ATCAACG231350.032.7759973
CAACGCA230900.032.7380755
TATCAAC236700.032.3249052
AACGCAG247800.030.8470736
TCAACGC246600.030.8444144
GTACATG222300.030.2359661
TACATGG225950.029.3852652
ACATGGG224350.028.5182653
GTACTAG26800.027.7690871
CATGGGG172350.027.4668754
ACGCAGA276250.027.4152137
CCTGGGG60900.027.0944844
ATGGGGG133450.026.8427775
CGCAGAG281750.026.8281848
GTGGTAT45100.025.9008731
TGGGGGG151350.025.1592276
Produced by FastQC (version 0.11.5)