##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211452_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5949012 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.380469563685534 31.0 31.0 34.0 29.0 34.0 2 31.423339371310732 31.0 31.0 34.0 28.0 34.0 3 31.486682158314693 31.0 31.0 34.0 28.0 34.0 4 35.133276752509495 37.0 35.0 37.0 32.0 37.0 5 35.18421075634072 37.0 35.0 37.0 32.0 37.0 6 35.25209446543393 37.0 35.0 37.0 32.0 37.0 7 35.18090533352429 37.0 35.0 37.0 32.0 37.0 8 35.18789035893691 37.0 35.0 37.0 32.0 37.0 9 36.76078179032081 39.0 37.0 39.0 32.0 39.0 10-11 36.68542003277183 39.0 37.0 39.0 32.0 39.0 12-13 36.58746612042471 39.0 37.0 39.0 32.0 39.0 14-15 37.75995997318546 40.0 37.0 41.0 32.0 41.0 16-17 37.74861682914743 40.0 37.0 41.0 32.0 41.0 18-19 37.74510019478865 40.0 37.0 41.0 32.0 41.0 20-21 37.72723000054462 40.0 37.0 41.0 32.0 41.0 22-23 37.6000170280376 40.0 37.0 41.0 32.0 41.0 24-25 37.501616486905725 40.0 37.0 41.0 32.0 41.0 26-27 37.35454273751675 40.0 36.5 41.0 31.0 41.0 28-29 37.22164344264224 40.0 36.0 41.0 31.0 41.0 30-31 37.05756846683113 39.0 36.0 41.0 31.0 41.0 32-33 36.8457815348162 39.0 35.5 41.0 30.0 41.0 34-35 36.72170454522532 39.0 35.0 41.0 30.0 41.0 36-37 36.57797185482228 39.0 35.0 41.0 30.0 41.0 38-39 36.414815687041816 39.0 35.0 40.0 30.0 41.0 40-41 36.23775721077718 39.0 35.0 40.0 29.0 41.0 42-43 36.02920182376502 38.0 35.0 40.0 28.0 41.0 44-45 35.873763071918496 38.0 35.0 40.0 27.5 41.0 46-47 35.62847452652642 38.0 34.5 40.0 27.0 41.0 48-49 35.361791504202714 38.0 34.0 40.0 26.0 41.0 50-51 34.67748274839587 37.5 33.5 39.5 25.0 40.5 52-53 34.97398492388316 38.0 33.5 39.5 26.0 40.5 54-55 35.366962194730824 38.0 34.0 40.0 26.0 41.0 56-57 35.26654636097557 38.0 34.0 40.0 26.0 41.0 58-59 35.03955043291222 38.0 34.0 40.0 26.0 41.0 60-61 34.79799166651538 37.0 34.0 40.0 26.0 41.0 62-63 34.497283918741466 37.0 33.0 40.0 25.5 41.0 64-65 34.27856625604386 36.0 33.0 40.0 25.0 41.0 66-67 34.072966906101385 36.0 33.0 39.0 25.0 41.0 68-69 33.729659647686034 35.5 33.0 39.0 24.5 41.0 70-71 33.359663671883666 35.0 32.5 38.5 24.0 40.5 72-73 32.9826461099759 35.0 32.0 37.5 23.0 40.0 74-75 32.60324890923064 35.0 32.0 37.0 22.5 39.5 76-77 32.22383178921139 35.0 31.0 36.5 21.0 39.0 78-79 31.83544317947249 35.0 31.0 36.0 20.0 39.0 80-81 31.450401680144537 35.0 31.0 35.5 19.5 37.5 82-83 31.089332816945067 34.5 30.0 35.0 18.5 37.0 84-85 30.76539490927233 34.0 30.0 35.0 18.0 37.0 86-87 30.434982565172167 34.0 29.5 35.0 17.0 36.0 88-89 30.129974355405572 34.0 29.0 35.0 15.0 36.0 90-91 29.824051035701387 34.0 29.0 35.0 13.5 35.5 92-93 29.520830517739753 34.0 28.5 35.0 10.5 35.0 94-95 29.229085350643096 34.0 27.0 35.0 10.0 35.0 96-97 28.93290944782092 34.0 27.0 35.0 10.0 35.0 98-99 28.60810215208845 34.0 25.5 35.0 10.0 35.0 100 27.343221361799237 32.0 24.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.29309624761839714 1101 2 -0.2679121407610765 1101 3 -0.3652410949351541 1101 4 -0.2043413941264518 1101 5 -0.19328335340070169 1101 6 -0.3644912077873883 1101 7 -0.3248168171294097 1101 8 -0.4150669697983602 1101 9 -0.6044595005016546 1101 10-11 -0.46493890708156016 1101 12-13 -0.4950337457423828 1101 14-15 -0.7253325697426405 1101 16-17 -0.6771880529634444 1101 18-19 -0.6565924685507198 1101 20-21 -0.6407217268058716 1101 22-23 -0.5908914116804311 1101 24-25 -0.6706193695322824 1101 26-27 -0.7323910851130009 1101 28-29 -0.772157462061152 1101 30-31 -0.7452188615963067 1101 32-33 -0.8122259997753574 1101 34-35 -0.8903183844428995 1101 36-37 -0.9236370321525769 1101 38-39 -0.9278879126101174 1101 40-41 -1.0300889480916737 1101 42-43 -1.0331651826617545 1101 44-45 -1.0779719470611298 1101 46-47 -1.0911151745900938 1101 48-49 -1.1782362704542564 1101 50-51 -1.0469219812923214 1101 52-53 -0.8332576264097327 1101 54-55 -0.8280516589229521 1101 56-57 -0.8793499194841345 1101 58-59 -0.8687936169351715 1101 60-61 -0.8446631172592802 1101 62-63 -1.0048047886038134 1101 64-65 -0.9278720584563942 1101 66-67 -0.9252207087903983 1101 68-69 -0.9406057296734431 1101 70-71 -0.8834501738272778 1101 72-73 -1.0937024524979435 1101 74-75 -1.1257996174141525 1101 76-77 -1.0284063243123072 1101 78-79 -1.1681881926676176 1101 80-81 -1.2779494702750362 1101 82-83 -1.361980499650187 1101 84-85 -1.3398802436519155 1101 86-87 -1.4939380137093394 1101 88-89 -1.7314890604075828 1101 90-91 -1.7738849416323355 1101 92-93 -1.907617402066922 1101 94-95 -1.9793823665422003 1101 96-97 -1.9432230800840244 1101 98-99 -2.1931792578421927 1101 100 -2.0515993669759673 1102 1 -0.3553673727067803 1102 2 -0.3910934795291574 1102 3 -0.47215210073388647 1102 4 -0.4324186772574876 1102 5 -0.290613117645961 1102 6 -0.2514090763415666 1102 7 -0.45201570791444823 1102 8 -0.41831405258410825 1102 9 -0.5912438076054656 1102 10-11 -0.4594126973152868 1102 12-13 -0.6145303109503644 1102 14-15 -0.7570912431667338 1102 16-17 -0.7622065274638459 1102 18-19 -0.7575225410767956 1102 20-21 -0.8413698319590495 1102 22-23 -0.7637267337953872 1102 24-25 -0.817255478675861 1102 26-27 -0.8764758151562262 1102 28-29 -0.9162403297555457 1102 30-31 -0.9353441914648428 1102 32-33 -0.9497551843335117 1102 34-35 -0.9877887948238921 1102 36-37 -0.9668718482673597 1102 38-39 -1.0979681612212744 1102 40-41 -1.2037227910671078 1102 42-43 -1.105122616816864 1102 44-45 -1.1181278349700747 1102 46-47 -1.256862319454136 1102 48-49 -1.3029301080661284 1102 50-51 -1.2043165692445328 1102 52-53 -1.0060582622777048 1102 54-55 -1.05017284009908 1102 56-57 -0.9655015944496412 1102 58-59 -0.8964293992365171 1102 60-61 -0.9605126214709827 1102 62-63 -1.0474376004587782 1102 64-65 -1.1348707343263769 1102 66-67 -1.1800262283577965 1102 68-69 -1.1013094399068635 1102 70-71 -0.9916861244228912 1102 72-73 -1.1336690404079448 1102 74-75 -1.3452761856515423 1102 76-77 -1.3222081480484391 1102 78-79 -1.375266755542686 1102 80-81 -1.4021444311936442 1102 82-83 -1.5150472605103147 1102 84-85 -1.502109411573084 1102 86-87 -1.5599681735174897 1102 88-89 -1.6582328319371094 1102 90-91 -1.6476186996225906 1102 92-93 -1.7294918841011544 1102 94-95 -1.8809234369209804 1102 96-97 -2.060621631735337 1102 98-99 -2.1213158723030823 1102 100 -2.1486413207288813 1103 1 -0.37234650736273167 1103 2 -0.3787858923346512 1103 3 -0.48799991350647076 1103 4 -0.2708862485322996 1103 5 -0.2660737732274612 1103 6 -0.3008672214148689 1103 7 -0.42287179917512674 1103 8 -0.36561463584679643 1103 9 -0.3762536624377901 1103 10-11 -0.41989946138541967 1103 12-13 -0.5551886675203477 1103 14-15 -0.6621331817361877 1103 16-17 -0.6830006296385278 1103 18-19 -0.6753051976748807 1103 20-21 -0.6759676428167367 1103 22-23 -0.631678778462458 1103 24-25 -0.6038649437574577 1103 26-27 -0.7408410064729409 1103 28-29 -0.6850636646136437 1103 30-31 -0.6923182508744858 1103 32-33 -0.8610565614364134 1103 34-35 -0.6988159240298799 1103 36-37 -0.8156814019857705 1103 38-39 -0.787554653498411 1103 40-41 -0.7719304325221756 1103 42-43 -0.8089654499348526 1103 44-45 -0.8622499782099737 1103 46-47 -0.9158680590935404 1103 48-49 -0.8423595671977537 1103 50-51 -0.8422815218577 1103 52-53 -0.6076057183583643 1103 54-55 -0.6843356282031792 1103 56-57 -0.6345735328873374 1103 58-59 -0.5993395620151247 1103 60-61 -0.5205395112381055 1103 62-63 -0.6084227053078877 1103 64-65 -0.514723814141476 1103 66-67 -0.5872661637292111 1103 68-69 -0.6677297919356349 1103 70-71 -0.7734512416253878 1103 72-73 -0.6884766643685012 1103 74-75 -0.7507547401699242 1103 76-77 -0.8457902731320459 1103 78-79 -0.8495980232880491 1103 80-81 -0.9008802728189984 1103 82-83 -0.9103573223661243 1103 84-85 -1.0481293376978407 1103 86-87 -1.0760632953984661 1103 88-89 -1.0409822144973226 1103 90-91 -1.048929171726197 1103 92-93 -1.2648666036451601 1103 94-95 -1.2808669972100972 1103 96-97 -1.4056945104646807 1103 98-99 -1.5723377539219285 1103 100 -1.4770568251544383 1104 1 -0.48275711460434323 1104 2 -0.47156252949795174 1104 3 -0.471186362344735 1104 4 -0.3244139138478488 1104 5 -0.3642706677381895 1104 6 -0.327871415198139 1104 7 -0.3241644405733126 1104 8 -0.46826821090787973 1104 9 -0.5271356715950404 1104 10-11 -0.49321892426175395 1104 12-13 -0.5680512413225713 1104 14-15 -0.7620082292440884 1104 16-17 -0.6807966085564559 1104 18-19 -0.7201165369848539 1104 20-21 -0.7305954988697607 1104 22-23 -0.7156002147117206 1104 24-25 -0.7333186188746552 1104 26-27 -0.7112390155269637 1104 28-29 -0.9019043804795146 1104 30-31 -0.919748771325807 1104 32-33 -0.9125574618899179 1104 34-35 -0.8565917902582143 1104 36-37 -1.0465398441353955 1104 38-39 -0.9675753573484371 1104 40-41 -0.9439957272211643 1104 42-43 -0.917982731010099 1104 44-45 -1.00059958843012 1104 46-47 -1.0206485249764867 1104 48-49 -1.042452373503231 1104 50-51 -1.0404970629213253 1104 52-53 -0.8333446670589453 1104 54-55 -0.8232281097233525 1104 56-57 -0.6983082742020841 1104 58-59 -0.8150766497367883 1104 60-61 -0.8140883480380481 1104 62-63 -0.8613090975815112 1104 64-65 -0.8172610100072646 1104 66-67 -0.9201510472202372 1104 68-69 -0.9334110024969036 1104 70-71 -0.8958453656377543 1104 72-73 -0.8451047492388426 1104 74-75 -0.9868816239331508 1104 76-77 -1.0562173946314672 1104 78-79 -1.0033606895804148 1104 80-81 -1.0756541521159484 1104 82-83 -1.218274041390444 1104 84-85 -1.2096278786565442 1104 86-87 -1.1847013955853072 1104 88-89 -1.1563587899417094 1104 90-91 -1.1906779544246682 1104 92-93 -1.209430350408205 1104 94-95 -1.2627208719101155 1104 96-97 -1.3616067728452066 1104 98-99 -1.5024420216811372 1104 100 -1.444880529055414 1105 1 -0.599753762686408 1105 2 -0.5971371277828297 1105 3 -0.5816864483754465 1105 4 -0.38435248640698205 1105 5 -0.46553908550043843 1105 6 -0.38216656057922904 1105 7 -0.46666016588861226 1105 8 -0.4656914343556977 1105 9 -0.5062831537497061 1105 10-11 -0.5696876292303372 1105 12-13 -0.5659514180189049 1105 14-15 -0.833893248965559 1105 16-17 -0.8308353926567094 1105 18-19 -0.7763596634482752 1105 20-21 -0.8450280929973388 1105 22-23 -0.7649904202433078 1105 24-25 -0.7597543568173961 1105 26-27 -0.8169149303808538 1105 28-29 -0.8259176204868837 1105 30-31 -0.8574236124357029 1105 32-33 -0.8915120327011934 1105 34-35 -0.858333668861853 1105 36-37 -0.8855944419560586 1105 38-39 -0.9304472851924857 1105 40-41 -0.8715439932608433 1105 42-43 -0.8614439212844403 1105 44-45 -0.915564538574472 1105 46-47 -0.9476756690892145 1105 48-49 -1.0730458599801196 1105 50-51 -0.9952559740197771 1105 52-53 -0.9194634984281507 1105 54-55 -0.8865900702230434 1105 56-57 -0.8476233118872827 1105 58-59 -0.7141421551126186 1105 60-61 -0.7942542867534783 1105 62-63 -0.832633281408846 1105 64-65 -0.7741269346593569 1105 66-67 -0.8889379558185695 1105 68-69 -0.8344644047537955 1105 70-71 -0.8286660671855799 1105 72-73 -0.8804750665750305 1105 74-75 -0.9031429815547014 1105 76-77 -0.9967644432663221 1105 78-79 -1.0732539920373618 1105 80-81 -1.0178222867589497 1105 82-83 -1.078239580090095 1105 84-85 -1.14778868335328 1105 86-87 -1.031572407834826 1105 88-89 -1.1846041462115657 1105 90-91 -1.2828885854273118 1105 92-93 -1.3602949860773386 1105 94-95 -1.4397821652050595 1105 96-97 -1.4556689382688184 1105 98-99 -1.4271338122465593 1105 100 -1.5912179742986865 1106 1 -0.46253541030959866 1106 2 -0.4970109699095637 1106 3 -0.545457439320554 1106 4 -0.3953426306226149 1106 5 -0.397905288333277 1106 6 -0.45684558702104283 1106 7 -0.4852681099328322 1106 8 -0.4463208957444067 1106 9 -0.5694505309528495 1106 10-11 -0.6534746140913938 1106 12-13 -0.6186487356410382 1106 14-15 -0.805165020268646 1106 16-17 -0.8416152680458566 1106 18-19 -0.8597963942138165 1106 20-21 -0.8601859306664537 1106 22-23 -0.8024463197496274 1106 24-25 -0.8309989696092472 1106 26-27 -0.8293220917397193 1106 28-29 -0.9019824497527722 1106 30-31 -0.875698244366852 1106 32-33 -0.8593502973493372 1106 34-35 -0.7916660096346035 1106 36-37 -0.7937096515437787 1106 38-39 -0.9199917033055556 1106 40-41 -0.9419855903113685 1106 42-43 -0.8776081298234715 1106 44-45 -0.9626727475296946 1106 46-47 -1.0144368857083705 1106 48-49 -1.0019060821220265 1106 50-51 -1.0185246232064245 1106 52-53 -0.8735634752912276 1106 54-55 -0.8488646905172743 1106 56-57 -0.8757386857608935 1106 58-59 -0.9243407664861181 1106 60-61 -0.8227328180102305 1106 62-63 -0.849387584330124 1106 64-65 -0.7922084108775991 1106 66-67 -0.771967514272859 1106 68-69 -0.7764066438883432 1106 70-71 -0.8663989766613156 1106 72-73 -0.8866623470137824 1106 74-75 -0.834753674649491 1106 76-77 -0.7763481736238873 1106 78-79 -0.7572850270110898 1106 80-81 -0.9368489886923221 1106 82-83 -0.8349468595978955 1106 84-85 -0.8770155197272729 1106 86-87 -0.8860858032956074 1106 88-89 -0.8615955136725582 1106 90-91 -0.8663002968208104 1106 92-93 -0.9413601302245169 1106 94-95 -0.9362628876248849 1106 96-97 -0.9539471023466142 1106 98-99 -1.0423004118298245 1106 100 -1.0317937826537218 1107 1 -0.5796969594933508 1107 2 -0.5375562266237175 1107 3 -0.5264146015653708 1107 4 -0.3873149484394389 1107 5 -0.4074563371613351 1107 6 -0.37710302305578836 1107 7 -0.40188244019751806 1107 8 -0.4520582779136717 1107 9 -0.5705567157702376 1107 10-11 -0.588892593269712 1107 12-13 -0.6854624528315227 1107 14-15 -0.8417234244119811 1107 16-17 -0.9231753489228254 1107 18-19 -0.8739742434797293 1107 20-21 -0.8938313792698054 1107 22-23 -0.8254831140449284 1107 24-25 -0.9151427296663712 1107 26-27 -0.9194159592915412 1107 28-29 -0.944853690391291 1107 30-31 -0.9403705988027511 1107 32-33 -0.9765154887529093 1107 34-35 -0.9817229792027078 1107 36-37 -1.0352704313332026 1107 38-39 -0.9584576954724895 1107 40-41 -0.9044324171832017 1107 42-43 -0.9957055162190258 1107 44-45 -1.0099071706414549 1107 46-47 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1.5492336196946113 2310 98-99 1.5795955362575604 2310 100 1.7329720624203304 2311 1 0.6347536368044722 2311 2 0.7159019725738993 2311 3 0.6905376119343067 2311 4 0.606884430933718 2311 5 0.5932628427579729 2311 6 0.6311183578357955 2311 7 0.6886869726304141 2311 8 0.6651879665491904 2311 9 0.8767525695874738 2311 10-11 0.6886010183885318 2311 12-13 0.8589859030500762 2311 14-15 1.1431330911286182 2311 16-17 1.0974430185773798 2311 18-19 1.0452037320296839 2311 20-21 1.1218986019801136 2311 22-23 1.152945476947842 2311 24-25 1.134367957119025 2311 26-27 1.1859891616057396 2311 28-29 1.2314728979218188 2311 30-31 1.2369437164471648 2311 32-33 1.3097464304191888 2311 34-35 1.2888921128720838 2311 36-37 1.320235584026591 2311 38-39 1.3427847068343226 2311 40-41 1.4126372572369803 2311 42-43 1.4665125210880063 2311 44-45 1.4436033848744856 2311 46-47 1.4393793921283233 2311 48-49 1.4895316419601912 2311 50-51 1.4081475350524215 2311 52-53 1.3281404963292331 2311 54-55 1.2961936339790157 2311 56-57 1.2795184722454636 2311 58-59 1.239818641179376 2311 60-61 1.2534476218221116 2311 62-63 1.2405310517351182 2311 64-65 1.225026730262627 2311 66-67 1.2552692049111016 2311 68-69 1.3045521123607173 2311 70-71 1.3047468093307018 2311 72-73 1.2984348604800289 2311 74-75 1.3577407706365747 2311 76-77 1.367090888639403 2311 78-79 1.340075347679612 2311 80-81 1.3435400937699278 2311 82-83 1.4120251900915477 2311 84-85 1.4383221816311043 2311 86-87 1.4406690444547365 2311 88-89 1.5471414832471027 2311 90-91 1.598481049439858 2311 92-93 1.6021765182630858 2311 94-95 1.7112985929588689 2311 96-97 1.7149151281235184 2311 98-99 1.7860367088084637 2311 100 1.9370354351415635 2312 1 0.5904985976198986 2312 2 0.6180280279608787 2312 3 0.6466403659580671 2312 4 0.45749798664404295 2312 5 0.5656693861938678 2312 6 0.3994127490021455 2312 7 0.5476112209928203 2312 8 0.5662039981330693 2312 9 0.7241995763369715 2312 10-11 0.7763234714400085 2312 12-13 0.8256229699930557 2312 14-15 1.047299067585179 2312 16-17 1.0082654773392505 2312 18-19 1.0495478437036425 2312 20-21 1.0594374485670315 2312 22-23 1.075777132871309 2312 24-25 1.1002357794016007 2312 26-27 1.1357367374918326 2312 28-29 1.201107398112896 2312 30-31 1.0603186413456314 2312 32-33 1.163489568787071 2312 34-35 1.1574819904303624 2312 36-37 1.1956041757490468 2312 38-39 1.2643853077441776 2312 40-41 1.2997850292289783 2312 42-43 1.3028975153263573 2312 44-45 1.280310338336676 2312 46-47 1.251773558148706 2312 48-49 1.3237459420724917 2312 50-51 1.2888281172009286 2312 52-53 1.1771945952987792 2312 54-55 1.1693393570597195 2312 56-57 1.1197360336310496 2312 58-59 1.165674466739823 2312 60-61 1.2010103203134506 2312 62-63 1.1301963783285487 2312 64-65 1.120272201841665 2312 66-67 1.0845485279406617 2312 68-69 1.1556883015126118 2312 70-71 1.144922992274367 2312 72-73 1.145916607940734 2312 74-75 1.1454904579067247 2312 76-77 1.125582776697975 2312 78-79 1.1698960166945405 2312 80-81 1.0295176826435402 2312 82-83 1.2046952319141297 2312 84-85 1.365942355061346 2312 86-87 1.4458154723062613 2312 88-89 1.4884050826639594 2312 90-91 1.515381717263896 2312 92-93 1.5681218360517661 2312 94-95 1.654475879425263 2312 96-97 1.6542193963307845 2312 98-99 1.5953795295414146 2312 100 1.7112705952987781 2313 1 0.6164302513070723 2313 2 0.6426553958557832 2313 3 0.7008270232835478 2313 4 0.545624564205049 2313 5 0.605353230440393 2313 6 0.6480459400333487 2313 7 0.7090333486467131 2313 8 0.6814440805299213 2313 9 0.8848384016195183 2313 10-11 0.7763524394116317 2313 12-13 0.9059739623154073 2313 14-15 1.1157545747419348 2313 16-17 1.132491418793066 2313 18-19 1.0960284465757297 2313 20-21 1.128887194292723 2313 22-23 1.1637139740448745 2313 24-25 1.16873926356552 2313 26-27 1.2381898329597263 2313 28-29 1.25188896986797 2313 30-31 1.2066702869971593 2313 32-33 1.2677350527156932 2313 34-35 1.2592209316441085 2313 36-37 1.2651109390146473 2313 38-39 1.2784500807027257 2313 40-41 1.3057612997151438 2313 42-43 1.3279345293721292 2313 44-45 1.3342086552841295 2313 46-47 1.3729814923345458 2313 48-49 1.4084310040644326 2313 50-51 1.3625098463263257 2313 52-53 1.210199743580823 2313 54-55 1.1784328989068413 2313 56-57 1.159233891637058 2313 58-59 1.2117412236791552 2313 60-61 1.1900079154169347 2313 62-63 1.1326229338536251 2313 64-65 1.129631506651684 2313 66-67 1.224426185902729 2313 68-69 1.2035597496905837 2313 70-71 1.2145739639957966 2313 72-73 1.2222835283616007 2313 74-75 1.3010326230567273 2313 76-77 1.3219561014513843 2313 78-79 1.3154919407746526 2313 80-81 1.407790860144356 2313 82-83 1.4845250437415132 2313 84-85 1.591067931887995 2313 86-87 1.6156523843480386 2313 88-89 1.6330154451732497 2313 90-91 1.7339055335381524 2313 92-93 1.8077532873018 2313 94-95 1.8836148118261704 2313 96-97 1.906790088561415 2313 98-99 2.0012275373495285 2313 100 2.0985849273902453 2314 1 0.4431560965913377 2314 2 0.480362355886907 2314 3 0.5572762425925504 2314 4 0.47871388797615566 2314 5 0.4540423226391397 2314 6 0.4899594909383538 2314 7 0.5523720285569667 2314 8 0.5292714543427408 2314 9 0.7136064508487721 2314 10-11 0.6900216057022845 2314 12-13 0.445803487732654 2314 14-15 0.80388137067456 2314 16-17 0.8278678093482625 2314 18-19 0.8576618390205937 2314 20-21 0.9014396758152472 2314 22-23 0.7677774896620235 2314 24-25 0.8459983964152187 2314 26-27 0.8749080690466329 2314 28-29 0.9274987434263622 2314 30-31 0.8436256561151936 2314 32-33 0.899878234376132 2314 34-35 0.9456522421279203 2314 36-37 0.9574726933617725 2314 38-39 0.9829654347288965 2314 40-41 1.0418459804958644 2314 42-43 1.1587936279920967 2314 44-45 1.1178053131420285 2314 46-47 1.1157008386309784 2314 48-49 1.1177432379904033 2314 50-51 1.0737182010748185 2314 52-53 0.9218377643652147 2314 54-55 0.9159046109100188 2314 56-57 0.8785973639823794 2314 58-59 0.8275078250631722 2314 60-61 0.8958562386261306 2314 62-63 0.9102653864488417 2314 64-65 0.8335331062295026 2314 66-67 0.8886730991961329 2314 68-69 0.9064349289601168 2314 70-71 0.9618010484615525 2314 72-73 0.9668489661270456 2314 74-75 1.0125905635780867 2314 76-77 1.0696964469423236 2314 78-79 1.1139604783510784 2314 80-81 1.1877356989205303 2314 82-83 1.144199002194938 2314 84-85 1.1604593574062072 2314 86-87 1.3683853799914054 2314 88-89 1.4916487708753117 2314 90-91 1.5318333489781715 2314 92-93 1.6313586938683144 2314 94-95 1.6964086151935902 2314 96-97 1.7277643340532798 2314 98-99 1.8490567332704337 2314 100 1.6923496846158947 2315 1 0.3490651898718511 2315 2 0.38456892633813666 2315 3 0.4724735630755177 2315 4 0.3586100178525058 2315 5 0.33129452789023617 2315 6 -0.3459562483837786 2315 7 0.17928309159320577 2315 8 0.20905282189116292 2315 9 0.4407455748465807 2315 10-11 0.5656467191023609 2315 12-13 0.6136399504733703 2315 14-15 0.671830059639241 2315 16-17 0.5449534911138869 2315 18-19 0.6746241864268399 2315 20-21 0.7019787935524988 2315 22-23 0.6267146765981053 2315 24-25 0.7083456287576979 2315 26-27 0.7024431803192854 2315 28-29 0.8195550208778783 2315 30-31 0.8513388540582483 2315 32-33 0.9063256628246705 2315 34-35 0.9107605960834064 2315 36-37 0.9439549330980839 2315 38-39 0.9541157303371719 2315 40-41 0.9795059361966452 2315 42-43 0.9255418050405595 2315 44-45 0.9709648929197385 2315 46-47 1.032662091889513 2315 48-49 1.0345638099383692 2315 50-51 0.9038977079634591 2315 52-53 0.783532710765023 2315 54-55 0.7016779153868313 2315 56-57 0.7063463050502818 2315 58-59 0.6570831232945054 2315 60-61 0.6129083351290845 2315 62-63 0.6921166785865722 2315 64-65 0.761785529150778 2315 66-67 0.7522047645556214 2315 68-69 0.8172700781438991 2315 70-71 0.8316073973587308 2315 72-73 0.9403655916653548 2315 74-75 0.9777405096387781 2315 76-77 0.948005555410468 2315 78-79 0.9590597400115008 2315 80-81 1.0224072980920589 2315 82-83 1.1629882719535019 2315 84-85 1.1523983146877832 2315 86-87 1.3196044344506888 2315 88-89 1.421599260328886 2315 90-91 1.4407692823542888 2315 92-93 1.5900489294912106 2315 94-95 1.7019143520057014 2315 96-97 1.7788376639780878 2315 98-99 1.9456942144525406 2315 100 1.910195074442612 2316 1 0.03158329857031461 2316 2 -0.023738374441656163 2316 3 0.08852197392220518 2316 4 0.08279108799037971 2316 5 0.07109955509993426 2316 6 0.1654743510569503 2316 7 0.1856726627059544 2316 8 0.18422161498508416 2316 9 0.30178057330370933 2316 10-11 0.20743614341347438 2316 12-13 0.298288298283218 2316 14-15 0.520493117681113 2316 16-17 0.5594123008221601 2316 18-19 0.5161511367038614 2316 20-21 0.43019016466571003 2316 22-23 0.3588947726077336 2316 24-25 0.4502150006774315 2316 26-27 0.5151918970311442 2316 28-29 0.6631717992984605 2316 30-31 0.6739191114132055 2316 32-33 0.7691701450002313 2316 34-35 0.7389318814358035 2316 36-37 0.8316085507540336 2316 38-39 0.8118960731629272 2316 40-41 0.7622731001421243 2316 42-43 0.8199539438128838 2316 44-45 0.6976210282109747 2316 46-47 0.6743555268239092 2316 48-49 0.7326029823473377 2316 50-51 0.4806609536444455 2316 52-53 0.2849558912212089 2316 54-55 0.19229236148681395 2316 56-57 0.30453125549406224 2316 58-59 0.38289236092187906 2316 60-61 0.4927017263258122 2316 62-63 0.49490083966234977 2316 64-65 0.47146764517567874 2316 66-67 0.48575342223975326 2316 68-69 0.5189360282889268 2316 70-71 0.45210426673492066 2316 72-73 0.5772363134606877 2316 74-75 0.6693882983058401 2316 76-77 0.7312484278860367 2316 78-79 0.8704416063840519 2316 80-81 1.0045519678640709 2316 82-83 1.0216647733062558 2316 84-85 1.0454302696339255 2316 86-87 1.1798797280379674 2316 88-89 1.3161816263046866 2316 90-91 1.2796460355132204 2316 92-93 1.4471898940359722 2316 94-95 1.6084665212856493 2316 96-97 1.682778980855307 2316 98-99 1.8450234830182914 2316 100 1.7703421511729083 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 21.0 1 316.0 2 182.0 3 113.0 4 95.0 5 100.0 6 111.0 7 406.0 8 1522.0 9 3124.0 10 4488.0 11 5979.0 12 7673.0 13 9322.0 14 11532.0 15 14296.0 16 19021.0 17 25380.0 18 32108.0 19 39908.0 20 48639.0 21 58214.0 22 63481.0 23 65345.0 24 66003.0 25 69526.0 26 78166.0 27 90581.0 28 107172.0 29 127708.0 30 154506.0 31 189638.0 32 239592.0 33 308301.0 34 421529.0 35 569419.0 36 807578.0 37 1112528.0 38 956166.0 39 239012.0 40 211.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.55460206661148 18.79262693616375 11.994857224295977 23.657913772928797 2 16.607295476617303 20.4946172010186 39.519395533331505 23.378691789032594 3 19.276147632134943 26.485852934817007 29.72586898164199 24.512130451406065 4 12.944211766960844 16.529565359207893 37.7564827907399 32.769740083091364 5 13.087744000258263 37.625553163653045 34.3021070955434 14.984595740545291 6 30.164746468985705 36.55772483905289 18.727048701406822 14.550479990554585 7 26.978078509383714 32.87406147288479 22.56463157023645 17.583228447495046 8 26.360345711437112 34.38401901991467 20.646113336410032 18.60952193223819 9 27.632967649454702 15.249969561845287 20.49501856895604 36.62204421974397 10-11 25.324412586315365 25.504455412493314 28.47250495807269 20.698627043118627 12-13 25.69267267890949 23.881978869002698 28.089671574474135 22.33567687761368 14-15 23.227363227074044 25.197435075754342 26.042313616950736 25.532888080220882 16-17 22.23945900669718 27.73128684299634 27.010777296223825 23.018476854082653 18-19 21.839360670029805 27.23716859077245 29.094592734982992 21.828878004214754 20-21 22.810473692192378 26.68154019821437 28.735535169717647 21.7724509398756 22-23 22.890747353070072 26.41971543186145 28.47423292815212 22.21530428691635 24-25 22.424302850136044 26.28922095855511 28.93870737183114 22.347768819477707 26-27 22.28970228305282 26.46112732379235 28.678425262248236 22.5707451309066 28-29 21.981825623737 27.30410056222558 28.561826109624445 22.152247704412975 30-31 22.948071392598855 26.630680801316952 28.63906734105769 21.782180465026507 32-33 22.818949759347838 26.66415524075767 28.302957325908125 22.213937673986372 34-35 22.81250168139117 26.728226320631883 28.652065689935323 21.807206308041625 36-37 22.481985130770948 26.557803318779644 28.64274884437078 22.31746270607863 38-39 22.484600124453287 26.606673971558003 28.758579282142264 22.150146621846446 40-41 22.343908376916474 25.946347065648396 29.214092783395085 22.49565177404004 42-43 22.080566897061498 26.37291073318422 28.611088411284534 22.93543395846974 44-45 21.59384027695682 26.742410955043283 29.016816412519965 22.646932355479933 46-47 21.631156951701282 26.313071289457472 28.6801118744794 23.37565988436184 48-49 21.94198954976792 26.338055750322475 28.680382200384035 23.039572499525566 50-51 21.745116327244705 26.51857131543518 28.59161534520524 23.144697012114875 52-53 21.372503003723534 26.898059395737178 28.354527918253314 23.374909682285974 54-55 21.242665319093568 26.55818535043978 28.666234759398083 23.532914571068574 56-57 21.81867623983587 26.398677237087714 28.180150770015477 23.602495753060936 58-59 21.407154725012997 26.431543291250193 28.550368042119523 23.610933941617287 60-61 21.75507051691669 26.509385511953713 28.21798138710858 23.517562584021018 62-63 21.884231093254 26.15361632078094 28.11093911426566 23.851213471699392 64-65 21.509490971076307 26.038135386608225 28.1060916609191 24.346281981396373 66-67 21.776773216438176 25.901887736627067 27.790364220651913 24.530974826282847 68-69 21.473970678384482 26.115543856347944 27.976044089959352 24.434441375308218 70-71 21.73356093685835 25.8324591640464 28.03161572381385 24.402364175281395 72-73 22.07836958490425 26.02729601270558 27.682828983027104 24.211505419363064 74-75 22.326303529541132 26.02535322901925 27.569593678809216 24.078749562630403 76-77 22.276994176426143 25.833521915488074 27.600786093363467 24.288697814722322 78-79 22.27567314197482 25.948851431960847 27.61342269106149 24.162052735002845 80-81 22.495670417584346 25.960782276373845 27.47603734491285 24.067509961128962 82-83 22.880827111249836 25.552354483359363 27.824921460898327 23.741896944492478 84-85 23.121913979910047 25.729206672868443 27.27765840186346 23.871220945358054 86-87 22.797848498129774 25.934057214119793 27.49627325297648 23.77182103477395 88-89 23.211154804443826 25.21419974749048 27.41762652313352 24.157018924932174 90-91 23.85900165077053 25.002603891487457 27.31213584924313 23.826258608498883 92-93 24.206972951897765 24.935442635954637 27.15731047943521 23.70027393271239 94-95 23.794179834976593 24.735604671513066 27.327557819520052 24.14265767399029 96-97 24.03375855186943 24.49408409877714 27.225806343137403 24.246351006216027 98-99 24.09346253886482 24.64810851865671 26.956246930286888 24.302182012191583 100 24.686149411729296 24.508939283293568 26.669914145820783 24.134997159156352 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3877.0 1 2587.5 2 2841.5 3 4097.5 4 2817.5 5 2038.5 6 2930.5 7 4733.0 8 6870.5 9 7831.0 10 6524.5 11 5147.5 12 5153.5 13 5550.5 14 6141.0 15 6830.0 16 7369.5 17 7827.5 18 8401.5 19 8990.5 20 9713.5 21 10596.0 22 11829.0 23 13608.0 24 15895.5 25 18720.0 26 22218.5 27 26646.5 28 32093.5 29 39964.0 30 50550.5 31 61242.5 32 74322.0 33 87470.0 34 100058.5 35 112787.0 36 125454.5 37 143181.0 38 154109.5 39 154968.5 40 154243.5 41 155655.5 42 161988.0 43 172511.5 44 187600.0 45 206288.5 46 223053.5 47 238220.5 48 253281.0 49 263215.5 50 266958.5 51 269541.5 52 266863.0 53 256088.0 54 242902.5 55 220684.0 56 197186.0 57 171672.5 58 144155.5 59 122275.0 60 97616.5 61 74875.5 62 58219.0 63 45171.0 64 33348.5 65 23230.0 66 16719.0 67 12766.0 68 9712.0 69 7315.0 70 5748.5 71 4395.0 72 3322.0 73 2598.5 74 2030.0 75 1535.0 76 1147.5 77 797.0 78 545.5 79 367.5 80 267.5 81 211.0 82 153.5 83 110.5 84 81.0 85 63.5 86 51.0 87 36.5 88 28.5 89 29.5 90 24.5 91 15.5 92 10.0 93 7.0 94 5.0 95 4.5 96 6.0 97 6.0 98 5.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21714530076590868 2 0.1462595805824564 3 0.0238358907327805 4 0.022558367675170263 5 0.027416317196872354 6 0.0549671105050721 7 0.06150601141836662 8 0.054530063143258076 9 0.042494451179456355 10-11 0.04452840236328318 12-13 0.04260371301990986 14-15 0.02074294017225045 16-17 0.019129226836321726 18-19 0.0184484415227268 20-21 0.02202046322986069 22-23 0.025357151742171645 24-25 0.0223734630220951 26-27 0.01957467895509372 28-29 0.019036774509784145 30-31 0.01728858506252803 32-33 0.0256765325065742 34-35 0.02615560365317804 36-37 0.025121818547348705 38-39 0.023516509968377942 40-41 0.02473519972728245 42-43 0.030761410466141267 44-45 0.0400234526338155 46-47 0.045469735142574935 48-49 0.041107666281392605 50-51 0.028492126087491503 52-53 0.04441914052282968 54-55 0.042351570311171 56-57 0.04488980691247555 58-59 0.04850385240439925 60-61 0.02466796167161875 62-63 0.02607996084055638 64-65 0.02639093684800098 66-67 0.03221543341986871 68-69 0.03648504995451345 70-71 0.040578166593041 72-73 0.04452840236328318 74-75 0.02563450872178439 76-77 0.018793036558003243 78-79 0.01822151308486182 80-81 0.02130605888843391 82-83 0.01981001214991666 84-85 0.012027207206843757 86-87 0.01336356356315973 88-89 0.018784631801045282 90-91 0.01989405971949628 92-93 0.020188226213024952 94-95 0.024062819170645476 96-97 0.024911699623399654 98-99 0.030173077479083925 100 0.03084545803572089 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5949012.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.4627370405312 #Duplication Level Percentage of deduplicated Percentage of total 1 76.49127560514647 47.01363158413916 2 14.170154778379551 17.418729939343383 3 4.386039879030115 8.087340472023337 4 1.8024378323917931 4.431310500968073 5 0.9289774119980274 2.854874719511399 6 0.5545674672532513 2.0451140646611994 7 0.3611954218972653 1.554004146542074 8 0.2484457773974091 1.2216125988005846 9 0.17519319002389128 0.9691067672757219 >10 0.8258609141384857 8.722400365051381 >50 0.03897508519285213 1.632927448272364 >100 0.015265175169600067 1.7072148221854964 >500 9.668789467356734E-4 0.3975466203572404 >1k 4.834372758578885E-4 0.5995961623677233 >5k 6.445830344436276E-5 0.25032530653943275 >10k+ 9.668745516654415E-5 1.0942644819612888 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23938 0.40238614411939333 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19840 0.3335007560919359 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11463 0.192687458018239 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6696 0.11255650518102837 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.680951391592419E-5 2 0.0 0.0 0.0 0.0 1.680951391592419E-5 3 0.0 0.0 0.0 0.0 1.680951391592419E-5 4 0.0 0.0 0.0 0.0 1.680951391592419E-5 5 0.0 0.0 0.0 0.0 5.042854174777257E-5 6 0.0 0.0 0.0 0.0 5.042854174777257E-5 7 1.680951391592419E-5 0.0 0.0 0.0 5.042854174777257E-5 8 1.680951391592419E-5 0.0 0.0 0.0 5.042854174777257E-5 9 1.680951391592419E-5 0.0 0.0 3.361902783184838E-5 5.042854174777257E-5 10-11 5.042854174777257E-5 0.0 0.0 8.404756957962095E-5 5.042854174777257E-5 12-13 5.042854174777257E-5 0.0 0.0 1.512856252433177E-4 1.1766659741146933E-4 14-15 5.042854174777257E-5 0.0 0.0 1.76499896117204E-4 2.0171416699109028E-4 16-17 5.042854174777257E-5 0.0 0.0 1.9330941003312818E-4 2.0171416699109028E-4 18-19 5.042854174777257E-5 0.0 0.0 3.1097600744459754E-4 2.1852368090701448E-4 20-21 5.042854174777257E-5 0.0 0.0 8.824994805860199E-4 2.6895222265478706E-4 22-23 5.042854174777257E-5 0.0 0.0 0.0026306889278421355 2.9416649352867333E-4 24-25 9.245232653758305E-5 0.0 0.0 0.007228090983847402 3.52999792234408E-4 26-27 1.0085708349554514E-4 0.0 0.0 0.015943823949254095 3.52999792234408E-4 28-29 1.0085708349554514E-4 0.0 0.0 0.043645902882697166 3.52999792234408E-4 30-31 1.344761113273935E-4 1.680951391592419E-5 0.0 0.12900461454776022 3.52999792234408E-4 32-33 1.512856252433177E-4 1.680951391592419E-5 0.0 0.32078435881453926 3.6140454919237014E-4 34-35 1.512856252433177E-4 1.680951391592419E-5 0.0 0.6217250864513301 3.782140631082943E-4 36-37 1.512856252433177E-4 1.680951391592419E-5 0.0 1.0712703218618487 4.0342833398218056E-4 38-39 1.512856252433177E-4 1.680951391592419E-5 0.0 1.6918859803947277 4.3704736181402896E-4 40-41 1.512856252433177E-4 1.680951391592419E-5 0.0 2.361745109944307 4.3704736181402896E-4 42-43 1.512856252433177E-4 3.361902783184838E-5 0.0 2.979116532291412 4.3704736181402896E-4 44-45 1.512856252433177E-4 3.361902783184838E-5 0.0 3.6891251858291763 4.454521187719911E-4 46-47 1.512856252433177E-4 3.361902783184838E-5 0.0 4.510278345379032 4.6226163268791524E-4 48-49 1.512856252433177E-4 3.361902783184838E-5 0.0 5.348022495163903 4.874759035618015E-4 50-51 1.512856252433177E-4 3.361902783184838E-5 0.0 6.18017748157173 5.042854174777257E-4 52-53 1.596903822012798E-4 3.361902783184838E-5 0.0 6.968484850929869 5.547139592254984E-4 54-55 1.680951391592419E-4 3.361902783184838E-5 0.0 7.8062037864438665 5.715234731414225E-4 56-57 1.680951391592419E-4 3.361902783184838E-5 0.0 8.72320479434232 5.715234731414225E-4 58-59 1.680951391592419E-4 3.361902783184838E-5 0.0 9.729657630544366 5.715234731414225E-4 60-61 1.680951391592419E-4 3.361902783184838E-5 0.0 10.709122792154394 5.715234731414225E-4 62-63 1.680951391592419E-4 3.361902783184838E-5 0.0 11.69108416658094 5.883329870573467E-4 64-65 1.680951391592419E-4 3.361902783184838E-5 0.0 12.626205830480759 5.883329870573467E-4 66-67 1.680951391592419E-4 6.723805566369676E-5 0.0 13.627674645806731 5.883329870573467E-4 68-69 1.849046530751661E-4 6.723805566369676E-5 0.0 14.715098910541784 5.883329870573467E-4 70-71 1.849046530751661E-4 6.723805566369676E-5 0.0 15.813810763871379 5.883329870573467E-4 72-73 1.849046530751661E-4 6.723805566369676E-5 0.0 16.84457015719585 6.051425009732708E-4 74-75 1.849046530751661E-4 6.723805566369676E-5 0.0 17.823396557277075 6.219520148891951E-4 76-77 1.849046530751661E-4 1.0085708349554514E-4 0.0 18.857148044078578 6.219520148891951E-4 78-79 1.849046530751661E-4 1.0926184045350723E-4 0.0 19.933251101191257 6.303567718471571E-4 80-81 1.9330941003312818E-4 1.1766659741146933E-4 0.0 21.00154613908999 6.387615288051192E-4 82-83 2.0171416699109028E-4 1.1766659741146933E-4 0.0 22.055116042798367 7.396186123006644E-4 84-85 2.0171416699109028E-4 1.1766659741146933E-4 0.0 23.048154886895503 7.396186123006644E-4 86-87 2.0171416699109028E-4 1.1766659741146933E-4 0.0 24.029132904757965 7.396186123006644E-4 88 2.0171416699109028E-4 1.1766659741146933E-4 0.0 24.790351742440592 7.396186123006644E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10785 0.0 53.238136 1 TACCTGG 4780 0.0 45.77623 2 GTATCAA 19265 0.0 40.195004 1 ACCTGGG 6200 0.0 34.347694 3 ATCAACG 23135 0.0 32.775997 3 CAACGCA 23090 0.0 32.738075 5 TATCAAC 23670 0.0 32.324905 2 AACGCAG 24780 0.0 30.847073 6 TCAACGC 24660 0.0 30.844414 4 GTACATG 22230 0.0 30.235966 1 TACATGG 22595 0.0 29.385265 2 ACATGGG 22435 0.0 28.518265 3 GTACTAG 2680 0.0 27.769087 1 CATGGGG 17235 0.0 27.466875 4 ACGCAGA 27625 0.0 27.415213 7 CCTGGGG 6090 0.0 27.094484 4 ATGGGGG 13345 0.0 26.842777 5 CGCAGAG 28175 0.0 26.828184 8 GTGGTAT 4510 0.0 25.900873 1 TGGGGGG 15135 0.0 25.159227 6 >>END_MODULE