FastQCFastQC Report
Mon 6 Jun 2016
ERR1211452_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211452_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5949012
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222880.3746504461581184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194240.3265079983029115No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121280.20386578477232858No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73160.12297840380890138No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106250.053.061481
TACCTGG52800.044.7709962
GTATCAA188250.040.089361
ACCTGGG61500.037.216123
ATCAACG238250.031.2463673
CAACGCA239050.031.1556975
TATCAAC243600.030.964242
AACGCAG255750.029.543376
TCAACGC255600.029.2178084
GTACATG226450.027.6372491
TACATGG230800.026.7764262
GTACTAG24950.026.572821
CCTGGGG61400.026.5610774
GTGGTAT42750.026.5075761
TGGGGGG131200.026.3951026
ACATGGG227400.026.1649863
ACGCAGA286950.025.9053767
CTGGGGG43200.025.561195
CGCAGAG292250.025.3551868
ATGGGGG130200.024.7937775
Produced by FastQC (version 0.11.5)