##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211452_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5949012 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.985752424100003 31.0 31.0 34.0 30.0 34.0 2 32.1188321691064 33.0 31.0 34.0 30.0 34.0 3 32.195003136655295 34.0 31.0 34.0 30.0 34.0 4 35.860165856111905 37.0 35.0 37.0 35.0 37.0 5 35.71299469558979 37.0 35.0 37.0 33.0 37.0 6 35.753414348466606 37.0 35.0 37.0 33.0 37.0 7 35.68166461926787 37.0 35.0 37.0 33.0 37.0 8 35.66901327480933 37.0 35.0 37.0 33.0 37.0 9 37.23582386453415 39.0 37.0 39.0 33.0 39.0 10-11 37.211899555758166 39.0 37.0 39.0 33.0 39.0 12-13 37.117342425935604 39.0 37.0 39.0 33.0 39.0 14-15 38.2898651910603 40.0 38.0 41.0 33.0 41.0 16-17 38.2762320869415 40.0 38.0 41.0 33.0 41.0 18-19 38.2787866287713 40.0 38.0 41.0 33.5 41.0 20-21 38.23703692310589 40.0 38.0 41.0 33.5 41.0 22-23 38.1313081903348 40.0 37.0 41.0 33.0 41.0 24-25 38.00609328069939 40.0 37.0 41.0 33.0 41.0 26-27 37.870451933867336 40.0 37.0 41.0 33.0 41.0 28-29 37.78345639578471 40.0 37.0 41.0 33.0 41.0 30-31 37.82279469263132 40.0 37.0 41.0 33.0 41.0 32-33 37.650267641080575 40.0 36.5 41.0 33.0 41.0 34-35 37.5293378127326 40.0 36.0 41.0 32.0 41.0 36-37 37.3735641313213 40.0 36.0 41.0 32.0 41.0 38-39 37.24905942364883 39.0 36.0 41.0 31.0 41.0 40-41 37.1699394790261 39.0 36.0 41.0 31.0 41.0 42-43 37.02099130074036 39.0 35.5 41.0 31.0 41.0 44-45 36.84963898206963 39.0 35.0 41.0 31.0 41.0 46-47 36.60577916131284 39.0 35.0 41.0 30.0 41.0 48-49 36.362049866431605 39.0 35.0 40.5 30.0 41.0 50-51 36.164487481282606 39.0 35.0 40.0 29.0 41.0 52-53 35.94129151193509 38.0 35.0 40.0 28.5 41.0 54-55 35.72522454148689 38.0 35.0 40.0 28.0 41.0 56-57 35.47513082844681 38.0 34.0 40.0 27.5 41.0 58-59 35.19182933233283 37.5 34.0 40.0 27.0 41.0 60-61 34.921244905876804 37.0 34.0 40.0 26.5 41.0 62-63 34.613929338182544 36.5 33.5 39.5 26.0 41.0 64-65 34.26000090435185 36.0 33.0 39.0 26.0 41.0 66-67 33.905103906329316 35.5 33.0 39.0 26.0 40.0 68-69 33.53749051102939 35.0 32.5 38.5 25.0 40.0 70-71 33.16963816848916 35.0 32.0 38.0 24.5 40.0 72-73 32.81889665040178 35.0 32.0 37.0 24.0 39.5 74-75 32.45214667578415 35.0 31.0 36.5 23.5 39.0 76-77 31.44536655834616 34.0 30.5 35.5 22.5 38.0 78-79 31.928391134527885 34.0 31.0 36.0 23.0 37.5 80-81 31.82169938470455 34.0 31.0 35.0 23.0 37.0 82-83 31.548162871414615 34.0 31.0 35.0 23.0 37.0 84-85 31.2676657905548 34.0 31.0 35.0 21.5 36.0 86-87 30.9985505996626 34.0 30.5 35.0 20.0 36.0 88-89 30.748521939441375 34.0 30.0 35.0 20.0 35.5 90-91 30.512851125531434 34.0 30.0 35.0 19.0 35.0 92-93 30.30820479098042 34.0 29.5 35.0 19.0 35.0 94-95 30.11608457000927 34.0 29.0 35.0 18.0 35.0 96-97 29.920573701986143 34.0 29.0 35.0 18.0 35.0 98-99 29.687236384798013 33.5 29.0 35.0 17.5 35.0 100 27.851083339552854 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.30268528413358453 1101 2 -0.2870442027670208 1101 3 -0.3508603854755492 1101 4 -0.2146071054562455 1101 5 -0.1793397195985591 1101 6 -0.26069946479209705 1101 7 -0.29801819376122296 1101 8 -0.37058159587729733 1101 9 -0.5491877905243285 1101 10-11 -0.48486854165263793 1101 12-13 -0.48947115817625786 1101 14-15 -0.5818349272101742 1101 16-17 -0.6995147763076446 1101 18-19 -0.6623829994992647 1101 20-21 -0.7420853831886447 1101 22-23 -0.7686662948757004 1101 24-25 -0.8324607653207821 1101 26-27 -0.7484467930606584 1101 28-29 -0.7792945048862876 1101 30-31 -0.7163583239991453 1101 32-33 -0.6388352781092053 1101 34-35 -0.7224594920113034 1101 36-37 -0.6278564623479781 1101 38-39 -0.7236127710573115 1101 40-41 -0.7915474749844904 1101 42-43 -0.7077690820433276 1101 44-45 -0.8047778024838195 1101 46-47 -0.8157480201869234 1101 48-49 -0.791938469429148 1101 50-51 -0.8582798968483587 1101 52-53 -0.8418743022885025 1101 54-55 -0.9398155758818874 1101 56-57 -1.0242686795621339 1101 58-59 -0.9013451973083377 1101 60-61 -0.7562113762533116 1101 62-63 -0.930695337431203 1101 64-65 -0.9249916088537375 1101 66-67 -0.9277574193959239 1101 68-69 -0.8495098442054356 1101 70-71 -0.86953436040573 1101 72-73 -1.0279946517171865 1101 74-75 -1.0316286045205914 1101 76-77 -0.8853196184711862 1101 78-79 -0.8438094103764371 1101 80-81 -0.8451846543398034 1101 82-83 -0.890665575212946 1101 84-85 -0.9190482570347065 1101 86-87 -1.038082074098444 1101 88-89 -1.1044129390661155 1101 90-91 -1.2686341668504504 1101 92-93 -1.362844452311915 1101 94-95 -1.4591952308802973 1101 96-97 -1.5987075875029007 1101 98-99 -1.745744649143127 1101 100 -1.5138901933896314 1102 1 -0.3320482397805087 1102 2 -0.28116538828862403 1102 3 -0.35376370970613635 1102 4 -0.2691183661484544 1102 5 -0.2702946637932797 1102 6 -0.28055986312368475 1102 7 -0.3390306845348334 1102 8 -0.36706400692375496 1102 9 -0.4948480987182293 1102 10-11 -0.4998246771298227 1102 12-13 -0.46249860485254857 1102 14-15 -0.6731056559286515 1102 16-17 -0.7242991085531614 1102 18-19 -0.7231795323023604 1102 20-21 -0.7347128485505081 1102 22-23 -0.7414124492805598 1102 24-25 -0.816524141982697 1102 26-27 -0.7563608444205201 1102 28-29 -0.832833891080945 1102 30-31 -0.7478707017902622 1102 32-33 -0.8091671378073002 1102 34-35 -0.8346467260446104 1102 36-37 -0.7055627345526005 1102 38-39 -0.8232669406390727 1102 40-41 -0.9290879888313626 1102 42-43 -0.8706805680176757 1102 44-45 -0.9723114442635037 1102 46-47 -1.0127545349935332 1102 48-49 -1.0606482465742815 1102 50-51 -1.1303285162581318 1102 52-53 -1.1819513042048584 1102 54-55 -1.1111306005673285 1102 56-57 -1.0508597229679069 1102 58-59 -1.11075681768218 1102 60-61 -1.134576446597329 1102 62-63 -1.1096187942734375 1102 64-65 -1.0678471110702432 1102 66-67 -1.2104123869941574 1102 68-69 -1.2110887497526832 1102 70-71 -1.2057078791710651 1102 72-73 -1.2959891705141828 1102 74-75 -1.1756289080834534 1102 76-77 -1.0674523291385327 1102 78-79 -0.9714234885541195 1102 80-81 -0.8615382110225625 1102 82-83 -1.0516882514827124 1102 84-85 -1.1356779534904682 1102 86-87 -1.187601409624797 1102 88-89 -1.2440659772709601 1102 90-91 -1.2419735569560402 1102 92-93 -1.4166179124753455 1102 94-95 -1.4156050553290882 1102 96-97 -1.5735706505452747 1102 98-99 -1.6245070205712189 1102 100 -1.4699313891665717 1103 1 -0.3223175311547948 1103 2 -0.30223018813886426 1103 3 -0.30873315405781554 1103 4 -0.15654594086701934 1103 5 -0.19899472975769328 1103 6 -0.2571967258034178 1103 7 -0.25447498228956533 1103 8 -0.2919835658782617 1103 9 -0.36635294984485256 1103 10-11 -0.4278689670411282 1103 12-13 -0.5070363805864133 1103 14-15 -0.6305418530510352 1103 16-17 -0.7571326240826792 1103 18-19 -0.7333596675462601 1103 20-21 -0.6644459446713498 1103 22-23 -0.82016512898916 1103 24-25 -0.7303675273573518 1103 26-27 -0.7676226673852611 1103 28-29 -0.7963734050828322 1103 30-31 -0.6899462362536752 1103 32-33 -0.678455704853711 1103 34-35 -0.6448214268072903 1103 36-37 -0.6471618231835947 1103 38-39 -0.7786473413031914 1103 40-41 -0.8328689477572553 1103 42-43 -0.8848164043119482 1103 44-45 -0.7480992257420596 1103 46-47 -0.7976165788303007 1103 48-49 -0.7717110886325571 1103 50-51 -0.7913015457589054 1103 52-53 -0.8620014500281883 1103 54-55 -0.817899915226775 1103 56-57 -0.7878765022254797 1103 58-59 -0.8547522521051931 1103 60-61 -0.8726414028564591 1103 62-63 -0.9473342318154323 1103 64-65 -0.9152618912046933 1103 66-67 -0.8624677545152153 1103 68-69 -0.8258290696356738 1103 70-71 -0.7844400694684452 1103 72-73 -0.8365100067517801 1103 74-75 -0.8316674937426711 1103 76-77 -0.8573834916104488 1103 78-79 -0.7829406478807002 1103 80-81 -0.7174818138990133 1103 82-83 -0.731536586179967 1103 84-85 -0.7757538899293301 1103 86-87 -0.8917467775165804 1103 88-89 -0.9060731092711762 1103 90-91 -1.12488021010914 1103 92-93 -1.0832682131450042 1103 94-95 -1.1621719683078382 1103 96-97 -1.2551983852880504 1103 98-99 -1.3627764879629503 1103 100 -1.2352354474334 1104 1 -0.41981503287698985 1104 2 -0.32458259637131803 1104 3 -0.35216789468874765 1104 4 -0.25007685903548804 1104 5 -0.26653664718654824 1104 6 -0.285387872675976 1104 7 -0.36640815851441744 1104 8 -0.3829688545862382 1104 9 -0.54707341412049 1104 10-11 -0.5120186691558288 1104 12-13 -0.6233197880439576 1104 14-15 -0.7237593054598648 1104 16-17 -0.7487098011430859 1104 18-19 -0.7505037795920444 1104 20-21 -0.6960720534923652 1104 22-23 -0.756811112638573 1104 24-25 -0.8209366876499828 1104 26-27 -0.8676573980605795 1104 28-29 -0.8756864185753344 1104 30-31 -0.802662461085518 1104 32-33 -0.758640716745667 1104 34-35 -0.8062312937013374 1104 36-37 -0.7845986824335114 1104 38-39 -0.8764908630488151 1104 40-41 -0.9463919705517085 1104 42-43 -0.7945945691543841 1104 44-45 -0.9309626465801202 1104 46-47 -0.8802369474394212 1104 48-49 -0.8832782637584629 1104 50-51 -0.9098636046265725 1104 52-53 -0.7912848393244687 1104 54-55 -0.7764409849580431 1104 56-57 -0.7953977319803514 1104 58-59 -0.8664960878790637 1104 60-61 -0.8058595934805552 1104 62-63 -0.8399620715453722 1104 64-65 -0.9261746465865528 1104 66-67 -0.948755817728788 1104 68-69 -0.7996967823425862 1104 70-71 -0.8108735066788597 1104 72-73 -0.7966139017269818 1104 74-75 -0.7091093300634341 1104 76-77 -0.6709951112657677 1104 78-79 -0.5689705342756923 1104 80-81 -0.7544963426959939 1104 82-83 -0.7854535424037259 1104 84-85 -0.7433632430285009 1104 86-87 -0.7113921856669023 1104 88-89 -0.8948277710013102 1104 90-91 -0.9252617107875416 1104 92-93 -1.0257051793773186 1104 94-95 -1.0479261893881642 1104 96-97 -1.2767221027695435 1104 98-99 -1.2922772059214154 1104 100 -1.2497926995197233 1105 1 -0.4088380620763239 1105 2 -0.4008182759703196 1105 3 -0.4278945622554886 1105 4 -0.21704971622025937 1105 5 -0.277809012918631 1105 6 -0.3306255376489631 1105 7 -0.3668426370281068 1105 8 -0.3354844797743297 1105 9 -0.5054188363243952 1105 10-11 -0.49112347834822145 1105 12-13 -0.5808237347523431 1105 14-15 -0.8076952742474717 1105 16-17 -0.802073241500743 1105 18-19 -0.6739145523381254 1105 20-21 -0.7731995583574118 1105 22-23 -0.8539616976654258 1105 24-25 -0.7801873605240317 1105 26-27 -0.8194434561550636 1105 28-29 -0.8019981958876343 1105 30-31 -0.7685719001213158 1105 32-33 -0.7418281282506243 1105 34-35 -0.7858923831608067 1105 36-37 -0.9061232057271553 1105 38-39 -0.7691212568806094 1105 40-41 -0.7459122313065762 1105 42-43 -0.7352933338658403 1105 44-45 -0.8187952515056978 1105 46-47 -0.8276241043050092 1105 48-49 -0.787908536058815 1105 50-51 -0.7952716896968539 1105 52-53 -0.8082906635350753 1105 54-55 -0.8284404222804369 1105 56-57 -0.8656664411729835 1105 58-59 -0.8949419061558146 1105 60-61 -0.8792563857857019 1105 62-63 -0.8304305752590295 1105 64-65 -0.9842183449974087 1105 66-67 -0.8861444184109999 1105 68-69 -0.9987666739511027 1105 70-71 -1.0283690896308144 1105 72-73 -1.0690881723455021 1105 74-75 -1.0511339696625157 1105 76-77 -1.012962841568033 1105 78-79 -0.9585850237070339 1105 80-81 -0.8268863197974774 1105 82-83 -0.8397671049163371 1105 84-85 -0.7895747633875736 1105 86-87 -0.932400142660633 1105 88-89 -0.8870230339709195 1105 90-91 -0.8233851132095076 1105 92-93 -0.9048872828559382 1105 94-95 -1.0557297010125453 1105 96-97 -1.0179271655993496 1105 98-99 -1.0925193930029948 1105 100 -1.089703784059548 1106 1 -0.41785386637897304 1106 2 -0.3590927401929598 1106 3 -0.3835202245329832 1106 4 -0.2750948989472377 1106 5 -0.23676173828464897 1106 6 -0.38717866222969377 1106 7 -0.3695777917443479 1106 8 -0.38742298482748794 1106 9 -0.5604479696889442 1106 10-11 -0.5065650172141503 1106 12-13 -0.635700835215907 1106 14-15 -0.6497713721563869 1106 16-17 -0.731307299608666 1106 18-19 -0.7997453084365489 1106 20-21 -0.7624139671883228 1106 22-23 -0.675248939223188 1106 24-25 -0.7660977405172957 1106 26-27 -0.6901119388653427 1106 28-29 -0.743366050478194 1106 30-31 -0.7432947703601087 1106 32-33 -0.7544014066029519 1106 34-35 -0.6717461016350299 1106 36-37 -0.6764189648237959 1106 38-39 -0.698395393900519 1106 40-41 -0.6690769867807163 1106 42-43 -0.6324798353156638 1106 44-45 -0.7416806696269802 1106 46-47 -0.7676219343622037 1106 48-49 -0.9356789198306572 1106 50-51 -0.9610847200322397 1106 52-53 -0.9594941603602507 1106 54-55 -1.0913840797498295 1106 56-57 -1.1237959744367672 1106 58-59 -1.0445090641741999 1106 60-61 -1.0800834625647084 1106 62-63 -1.0062036943909476 1106 64-65 -0.9421597709167955 1106 66-67 -1.029186003961506 1106 68-69 -0.9661723019749857 1106 70-71 -0.9955291102486967 1106 72-73 -1.083827440369717 1106 74-75 -1.0060456899548385 1106 76-77 -0.9951933374102317 1106 78-79 -0.9065905929987181 1106 80-81 -0.8525480667862553 1106 82-83 -0.8663018260547268 1106 84-85 -0.757494127080939 1106 86-87 -0.8949867521284283 1106 88-89 -0.9511175141019095 1106 90-91 -0.9702488271111065 1106 92-93 -0.9119264883146059 1106 94-95 -0.9220346306844931 1106 96-97 -0.8987068801252356 1106 98-99 -0.9662135768717626 1106 100 -0.980311046317329 1107 1 -0.35926118074388924 1107 2 -0.3385712924401112 1107 3 -0.4117305952125214 1107 4 -0.18680424193015455 1107 5 -0.23412362268387454 1107 6 -0.27928492186024556 1107 7 -0.3949821765832624 1107 8 -0.44053370306011175 1107 9 -0.49933670545147635 1107 10-11 -0.5361123778844785 1107 12-13 -0.6019027415078284 1107 14-15 -0.6980488810880345 1107 16-17 -0.6928972368443524 1107 18-19 -0.7278153224016748 1107 20-21 -0.7474474132664071 1107 22-23 -0.7131678929413994 1107 24-25 -0.7698343135231269 1107 26-27 -0.7312396062171374 1107 28-29 -0.7885437022111006 1107 30-31 -0.7988956185051279 1107 32-33 -0.8153122503116847 1107 34-35 -0.7976724395437813 1107 36-37 -0.8833694194778303 1107 38-39 -0.7979130130568919 1107 40-41 -0.8679327063127289 1107 42-43 -0.9179846863587073 1107 44-45 -0.9243431290375526 1107 46-47 -0.9685295689799673 1107 48-49 -0.9514015460877161 1107 50-51 -0.8284231629734151 1107 52-53 -0.8909108657460081 1107 54-55 -0.9503776228487766 1107 56-57 -0.9198871342770545 1107 58-59 -0.953132413362674 1107 60-61 -0.9361268896376203 1107 62-63 -0.9590169154520396 1107 64-65 -0.9151147629236007 1107 66-67 -0.9233618811786712 1107 68-69 -0.8989643390980646 1107 70-71 -0.9587685300922928 1107 72-73 -0.9504456589501586 1107 74-75 -0.9509483704973754 1107 76-77 -0.9210805952846428 1107 78-79 -0.854046157916855 1107 80-81 -0.8523408704161355 1107 82-83 -0.8848792307729454 1107 84-85 -0.8049311035528568 1107 86-87 -0.8674534898019743 1107 88-89 -0.8336882289482546 1107 90-91 -0.7733481264923867 1107 92-93 -0.760189674462886 1107 94-95 -0.8918885022001035 1107 96-97 -0.9387126945518389 1107 98-99 -0.8570077678264596 1107 100 -0.8498480118953964 1108 1 -0.372838268796599 1108 2 -0.3475339568043836 1108 3 -0.4249946834918461 1108 4 -0.263274368629439 1108 5 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1.0408525975657454 2311 38-39 1.028661996393879 2311 40-41 1.0130947801232892 2311 42-43 1.1871539322953097 2311 44-45 1.1489157677814958 2311 46-47 1.1240451110349525 2311 48-49 1.1346840933491578 2311 50-51 1.1313160525779722 2311 52-53 1.1269241853449472 2311 54-55 1.1959264918807335 2311 56-57 1.2239488748285012 2311 58-59 1.2293250139957692 2311 60-61 1.2062462602045727 2311 62-63 1.2158355799614213 2311 64-65 1.2406464326887203 2311 66-67 1.25847880692573 2311 68-69 1.2942869087506566 2311 70-71 1.3445119820100615 2311 72-73 1.3085557212724623 2311 74-75 1.2194954528013113 2311 76-77 1.1154022794155054 2311 78-79 1.0435699887313206 2311 80-81 1.0715921518674847 2311 82-83 1.1021324389556248 2311 84-85 1.1479761930725694 2311 86-87 1.2310674442054648 2311 88-89 1.2927602443185329 2311 90-91 1.3599827034650716 2311 92-93 1.4187997905320309 2311 94-95 1.4941832403451514 2311 96-97 1.520829301261422 2311 98-99 1.5628212284607628 2311 100 1.6185758647567532 2312 1 0.18820607990506133 2312 2 0.16772337094183598 2312 3 0.34846323131578316 2312 4 0.26881173454789575 2312 5 0.33191646026140376 2312 6 0.43087574294620623 2312 7 0.4829129663084828 2312 8 0.3014547555291145 2312 9 0.3941639192818229 2312 10-11 0.26692513485927094 2312 12-13 0.45250855147610025 2312 14-15 0.761915795798366 2312 16-17 0.8946637899971179 2312 18-19 0.8986869989949682 2312 20-21 0.9008480113090513 2312 22-23 0.5167205395127183 2312 24-25 0.5056694431096886 2312 26-27 0.8726260501559722 2312 28-29 0.9816101595012796 2312 30-31 0.937315182927712 2312 32-33 0.7902805404890572 2312 34-35 0.9852863299359811 2312 36-37 1.0615090398494544 2312 38-39 1.0149202929471883 2312 40-41 1.042066005072563 2312 42-43 0.968329025382161 2312 44-45 0.8506696905095268 2312 46-47 1.0274206313769128 2312 48-49 1.1014860786163254 2312 50-51 1.0619150150420964 2312 52-53 1.150004414522357 2312 54-55 1.1249731136418504 2312 56-57 1.1323272723338818 2312 58-59 1.1313001390999133 2312 60-61 1.1561097230598776 2312 62-63 1.1373705259054034 2312 64-65 1.2207169488162108 2312 66-67 1.2289475820155005 2312 68-69 1.2151288560851512 2312 70-71 1.0852817830684813 2312 72-73 1.2124913489504294 2312 74-75 1.2453669551198772 2312 76-77 1.1521902424765358 2312 78-79 1.0774240357703775 2312 80-81 0.9783140353430966 2312 82-83 1.0322675118950109 2312 84-85 0.9120160624068951 2312 86-87 1.1335939695047301 2312 88-89 0.8631488980940105 2312 90-91 1.1464643791797116 2312 92-93 1.4035040869496562 2312 94-95 1.4736285310460318 2312 96-97 1.5678245323447442 2312 98-99 1.5951416473406148 2312 100 1.8169578593265037 2313 1 -0.2752665802515146 2313 2 -4.88397711826849E-4 2313 3 0.28745762061765845 2313 4 0.15276250055271134 2313 5 0.31611768442033394 2313 6 0.4239256109649503 2313 7 0.17502632506079152 2313 8 0.1339648136240541 2313 9 0.3843569789499739 2313 10-11 0.47050207091232465 2313 12-13 0.6246181003852769 2313 14-15 0.7155109133672894 2313 16-17 0.869236846771912 2313 18-19 0.7664943678767742 2313 20-21 0.9360903231299815 2313 22-23 0.968538958011095 2313 24-25 0.810333780182539 2313 26-27 0.9797967539163892 2313 28-29 0.8472099355180163 2313 30-31 0.8350987580839728 2313 32-33 0.9207015333217115 2313 34-35 0.966213168798042 2313 36-37 0.7972455999877752 2313 38-39 0.6269519046193253 2313 40-41 0.7473617520864266 2313 42-43 0.7976006211437223 2313 44-45 1.0018695873269152 2313 46-47 1.0517193639477966 2313 48-49 1.0229169226831303 2313 50-51 0.9908953640617639 2313 52-53 0.960969381089896 2313 54-55 0.9771125791567457 2313 56-57 1.0184731753586433 2313 58-59 0.9435896824332346 2313 60-61 1.0096467210048914 2313 62-63 1.0369994125141488 2313 64-65 1.097069285757577 2313 66-67 1.1192530580776108 2313 68-69 1.1927333996100842 2313 70-71 1.0167982550435255 2313 72-73 1.1354551995048539 2313 74-75 0.7495660577671259 2313 76-77 0.9554671112890603 2313 78-79 0.8545328577059266 2313 80-81 0.9814527552484336 2313 82-83 0.9475079731332308 2313 84-85 1.0572737557615461 2313 86-87 1.1488003656328658 2313 88-89 1.1839838495551511 2313 90-91 1.3223165842823654 2313 92-93 1.0764422652610683 2313 94-95 1.114317292213503 2313 96-97 1.4021871630184712 2313 98-99 1.4686196975680552 2313 100 1.4772534791989074 2314 1 -0.27839487160767007 2314 2 -0.12328912250017865 2314 3 0.1403168615285395 2314 4 -0.11029733429511168 2314 5 0.19027341017579147 2314 6 0.2684306065378763 2314 7 0.14001286254930534 2314 8 0.1657935271765325 2314 9 0.2279892309956537 2314 10-11 0.48650872624997277 2314 12-13 0.5806519918916706 2314 14-15 0.6179972658702084 2314 16-17 0.8117570227805189 2314 18-19 0.8382346757046335 2314 20-21 0.4959096506614742 2314 22-23 0.6991534113350113 2314 24-25 0.9063547419359566 2314 26-27 0.8512278361279826 2314 28-29 0.7797623941023275 2314 30-31 0.7895011691565941 2314 32-33 0.8617600549198059 2314 34-35 0.9284601806517756 2314 36-37 0.8248771199344702 2314 38-39 0.7990112589485747 2314 40-41 0.838324837767459 2314 42-43 0.701819354471894 2314 44-45 0.7054789744134098 2314 46-47 0.8550218155396507 2314 48-49 0.8312751877580169 2314 50-51 0.9228788439604116 2314 52-53 0.9871554698625573 2314 54-55 1.021732509978058 2314 56-57 0.8750172645288004 2314 58-59 0.9179464801283999 2314 60-61 1.0115771196577228 2314 62-63 0.704310519655877 2314 64-65 0.8521348849784474 2314 66-67 0.9469497262503097 2314 68-69 0.992904609325592 2314 70-71 1.1099589603151614 2314 72-73 1.042746173554633 2314 74-75 1.07913152439032 2314 76-77 0.8943518209423402 2314 78-79 0.9189575957996254 2314 80-81 0.9078511316372868 2314 82-83 0.8562405491552774 2314 84-85 0.7728748061393382 2314 86-87 0.924862569554179 2314 88-89 1.0177588853202622 2314 90-91 1.1368020623953896 2314 92-93 1.2106224620206234 2314 94-95 1.3641448486440986 2314 96-97 1.3404457334379707 2314 98-99 1.3897867646422029 2314 100 1.4250077393061105 2315 1 -0.1603812583812072 2315 2 0.04789949001617089 2315 3 0.10592106004142465 2315 4 -0.07037108709714346 2315 5 0.21537989755874776 2315 6 0.35422077248312434 2315 7 0.4192287078950798 2315 8 0.4103722788568831 2315 9 0.610925352478219 2315 10-11 0.6319858818948774 2315 12-13 0.6650191498958691 2315 14-15 0.7434886541306227 2315 16-17 0.7307963596371607 2315 18-19 0.7818338608471791 2315 20-21 0.7981736814031066 2315 22-23 0.8781669190595522 2315 24-25 0.7970997087115421 2315 26-27 0.9177257504360696 2315 28-29 0.9173623383370284 2315 30-31 0.8658164144166847 2315 32-33 0.9556266251275716 2315 34-35 0.8396779916955026 2315 36-37 0.9016056414525195 2315 38-39 0.9284996557656555 2315 40-41 0.831320517115806 2315 42-43 0.9283033665825897 2315 44-45 0.8054325564646305 2315 46-47 0.7944680579610477 2315 48-49 0.890386373164894 2315 50-51 0.7989790783254023 2315 52-53 0.8543382191963644 2315 54-55 0.7589964131898981 2315 56-57 0.8573519598224308 2315 58-59 0.8576306815628811 2315 60-61 0.9260657802296279 2315 62-63 0.9006464870592623 2315 64-65 0.8398221364040239 2315 66-67 0.8758761369182935 2315 68-69 0.8434048667039136 2315 70-71 0.38746967426755674 2315 72-73 0.7029562093383319 2315 74-75 0.8851581914511684 2315 76-77 0.8410338940783575 2315 78-79 0.7257459273446472 2315 80-81 0.7665034778667774 2315 82-83 0.8018622409888003 2315 84-85 0.8031647639791473 2315 86-87 0.7684418716415493 2315 88-89 0.8441974939237724 2315 90-91 0.9230939276752252 2315 92-93 1.059098763505709 2315 94-95 1.18608904624109 2315 96-97 1.2354784549760716 2315 98-99 1.148263619722556 2315 100 0.9862785621689589 2316 1 0.4794314138585527 2316 2 0.4797432473533263 2316 3 0.4770936669071091 2316 4 0.2823122758033705 2316 5 0.4093160772316651 2316 6 0.44787521627146987 2316 7 0.48321606342053514 2316 8 0.5077383474198953 2316 9 0.5185139991074479 2316 10-11 0.5017552472251694 2316 12-13 0.6392753375187681 2316 14-15 0.788977862482966 2316 16-17 0.8062703195132244 2316 18-19 0.8580577960395104 2316 20-21 0.8992176011944863 2316 22-23 0.9267783278328068 2316 24-25 0.9643863212083161 2316 26-27 1.0061596356403513 2316 28-29 0.9488604486178147 2316 30-31 0.8293276295798222 2316 32-33 0.7671134587224557 2316 34-35 0.73800567602337 2316 36-37 0.7011638024314806 2316 38-39 0.5791908752407195 2316 40-41 0.68344964199337 2316 42-43 0.8541720584465082 2316 44-45 0.8005362985629034 2316 46-47 0.9014920812684792 2316 48-49 0.5978176302701357 2316 50-51 0.6651786304567295 2316 52-53 0.817479098227679 2316 54-55 0.8885911615553894 2316 56-57 0.9790176284768037 2316 58-59 0.8549079259656409 2316 60-61 0.8847721153259016 2316 62-63 0.873767142480375 2316 64-65 0.9347083949902881 2316 66-67 0.9569636760311013 2316 68-69 0.9197998287890456 2316 70-71 0.778220355442933 2316 72-73 0.8185820376832069 2316 74-75 0.8376433700090047 2316 76-77 0.7054355579866325 2316 78-79 0.7909432128365097 2316 80-81 0.727612666169307 2316 82-83 0.6987089539777322 2316 84-85 0.7949980965661041 2316 86-87 0.8192235039930154 2316 88-89 0.6927586982004712 2316 90-91 0.8508163527342063 2316 92-93 0.8948452926940504 2316 94-95 0.8879970090357006 2316 96-97 1.0486416506711436 2316 98-99 1.1720248156398334 2316 100 0.8512062883792133 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 9.0 12 36.0 13 226.0 14 869.0 15 2460.0 16 5645.0 17 11105.0 18 18352.0 19 25451.0 20 34007.0 21 45002.0 22 53151.0 23 54659.0 24 55794.0 25 62004.0 26 71838.0 27 87764.0 28 106785.0 29 129648.0 30 158885.0 31 195772.0 32 244884.0 33 315923.0 34 436620.0 35 592727.0 36 870005.0 37 1250037.0 38 988453.0 39 130886.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.193779310843574 19.816194679921594 12.980432949067142 24.009593060167695 2 16.159960679185048 21.005521589131103 39.33639737152993 23.49812036015392 3 18.79589733486793 26.514776994024718 30.437227297108716 24.25209837399864 4 12.745441535856422 17.02611744072349 37.79214587085259 32.436295152567496 5 12.826264925354316 37.86555876256772 34.41076054377628 14.897415768301684 6 30.079991447317884 37.04993989757289 18.428777489233084 14.441291165876144 7 26.713694138767746 33.09674474975227 22.47686798044379 17.712693131036197 8 26.28455106207193 35.000374013382114 20.305665588331454 18.409409336214498 9 27.272446090535258 15.36821289416261 20.402883957377732 36.9564570579244 10-11 25.28133876015786 25.85235386244368 28.312217902704788 20.554089474693672 12-13 25.549282222817283 23.96743414135271 28.09346996826857 22.38981366756144 14-15 23.051869789470924 25.37893687220668 25.948107013399873 25.621086324922526 16-17 22.14632999193479 27.879741590443853 26.95527622597792 23.018652191643444 18-19 21.757436360861266 27.321150133837353 29.113010698247038 21.808402807054346 20-21 22.646174074465986 26.67955003953178 28.79065706966611 21.883618816336124 22-23 22.729456818162145 26.44264513524642 28.554357307932587 22.273540738658852 24-25 22.263191690718287 26.343357970485847 28.996009420054765 22.3974409187411 26-27 22.161822837136654 26.570546504192627 28.737906395213187 22.529724263457528 28-29 21.88090440131304 27.33039365589099 28.657896382421644 22.13080556037433 30-31 22.838012033524834 26.687850749126298 28.67781925372378 21.796317963625093 32-33 22.789169712901717 26.65555628313456 28.335630530937188 22.21964347302654 34-35 22.70670042230983 26.739622682749776 28.75481440350996 21.798862491430434 36-37 22.413560162197733 26.615177885404783 28.669460537257496 22.301801415139984 38-39 22.40458744255441 26.57323663430625 28.8381825756809 22.18399334745844 40-41 22.24085279499775 25.941573739330774 29.347413339680617 22.47016012599086 42-43 22.0171785716507 26.34805493364093 28.729889427044192 22.90487706766418 44-45 21.505638995202865 26.71247920359684 29.109458783720687 22.672423017479613 46-47 21.521730541786205 26.262723397602127 28.750873164043885 23.46467289656778 48-49 21.815691426747254 26.316638401299514 28.737174784025672 23.13049538792756 50-51 21.604896035978726 26.450246150059748 28.5704871424335 23.374370671528027 52-53 21.225467507923984 26.799315611203006 28.355338469667778 23.619878411205228 54-55 21.072274983598607 26.4343055370516 28.635509132455596 23.8579103468942 56-57 21.590956751115407 26.259677209094118 28.097793257184538 24.05157278260594 58-59 21.008179027107136 26.461118731137073 28.397797968465028 24.13290427329076 60-61 21.48023930071972 26.656459205638537 27.88293464868771 23.980366844954034 62-63 21.461213388558672 26.39460510744735 27.735394085688924 24.408787418305046 64-65 20.92814153548958 26.252341684869844 27.77311180444551 25.04640497519506 66-67 21.17010401987821 26.194823550304246 27.516393710727886 25.118678719089655 68-69 20.88637883979278 26.42828581651741 27.600543401709842 25.084791941979965 70-71 21.032471145462555 26.2070782948274 27.58038955149621 25.180061008213833 72-73 21.139658247144002 26.56552925616666 27.1667287459673 25.12808375072204 74-75 21.294864837485093 26.759340566493385 26.874097028480197 25.071697567541325 76-77 21.24276432487756 26.533696620909346 26.83880706959708 25.384731984616018 78-79 21.059396435442228 26.559730437788943 26.95438865298161 25.426484473787227 80-81 20.886658070663117 26.91517919692057 26.80575539447411 25.392407337942206 82-83 21.259160891201894 26.44213995660982 27.07116806270407 25.227531089484213 84-85 21.53155756031531 26.561556922943417 26.483466253681076 25.423419263060193 86-87 21.003985127728335 26.815605206220038 26.668848439941016 25.51156122611061 88-89 21.127347459691038 26.245606817573268 26.719597899345665 25.90744782339003 90-91 21.364966826181025 26.017910852329294 26.756921000119554 25.86020132137012 92-93 21.41620189906476 26.020072445486186 26.7177934147697 25.845932240679346 94-95 20.850219393045773 25.826255232244456 27.074034005312704 26.249491369397067 96-97 20.910884474900314 25.65063026688035 27.11568462793985 26.322800630279485 98-99 20.836829432959753 25.956363464563065 26.89255736608751 26.314249736389673 100 20.80117352342844 25.955631399317408 26.94492173708368 26.298273340170482 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3841.0 1 2579.0 2 2474.5 3 3577.0 4 2639.5 5 1889.0 6 2683.0 7 4455.5 8 6236.0 9 6984.0 10 5964.5 11 4660.0 12 4634.5 13 5111.5 14 5656.5 15 6238.0 16 6951.0 17 7627.0 18 8298.0 19 9105.5 20 9989.0 21 10982.0 22 12344.5 23 14204.5 24 16655.0 25 19648.5 26 23531.0 27 28348.0 28 33909.5 29 41571.0 30 52063.5 31 62477.5 32 75500.0 33 88990.5 34 101006.5 35 113401.5 36 125827.5 37 143037.5 38 153960.0 39 155205.0 40 155903.5 41 158200.5 42 165271.0 43 176463.0 44 190996.5 45 211366.0 46 229506.5 47 245095.5 48 260681.0 49 269722.0 50 272784.0 51 274942.0 52 268843.0 53 253792.0 54 239089.5 55 216109.0 56 190457.5 57 163385.5 58 136291.5 59 115241.0 60 91953.5 61 70461.0 62 54762.0 63 42150.0 64 30943.5 65 21445.0 66 14992.5 67 11257.0 68 8673.0 69 6469.0 70 4997.0 71 3807.0 72 2902.5 73 2372.5 74 1833.0 75 1250.5 76 801.0 77 515.0 78 335.5 79 215.0 80 142.0 81 92.0 82 59.5 83 34.5 84 20.5 85 14.0 86 12.5 87 8.0 88 6.5 89 5.0 90 1.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.051218588901821 2 0.0 3 1.680951391592419E-5 4 0.016069895303623526 5 0.004067902367653654 6 1.680951391592419E-5 7 1.1766659741146933E-4 8 4.538568757299531E-4 9 0.0017313799333401917 10-11 7.564281262165886E-4 12-13 3.52999792234408E-4 14-15 0.0 16-17 1.0085708349554515E-4 18-19 0.0 20-21 8.404756957962096E-6 22-23 8.404756957962096E-6 24-25 3.361902783184838E-5 26-27 0.0 28-29 0.0010085708349554514 30-31 8.152614249223233E-4 32-33 0.013825825195847647 34-35 0.022936581738278557 36-37 0.030702577167435532 38-39 0.020339511838268272 40-41 1.512856252433177E-4 42-43 0.0014960467385172528 44-45 0.009539399147286978 46-47 0.013733372869310064 48-49 0.005555544349212945 50-51 0.010220184460881908 52-53 0.01564965745572542 54-55 0.00982516088385769 56-57 0.0029668792061606193 58-59 0.008488804527541716 60-61 0.0016053085789707604 62-63 8.740947236280579E-4 64-65 0.01856610812013827 66-67 0.04371314093836086 68-69 0.052689421369464376 70-71 0.0464530917066565 72-73 0.03894764374319635 74-75 0.02747515049557809 76-77 0.02786176931564435 78-79 0.011775064498104896 80-81 0.0020759749686166374 82-83 0.026609460528907995 84-85 0.045705068337397875 86-87 0.04670523441539536 88-89 0.05775748981511552 90-91 0.033669456373596154 92-93 0.025306723200423868 94-95 0.0074214003938805305 96-97 0.0037401168462931327 98-99 0.0220624870146505 100 0.0186921794745077 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5949012.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.821318251599486 #Duplication Level Percentage of deduplicated Percentage of total 1 75.93268896653525 46.18326241333357 2 14.45451488448326 17.582852999232763 3 4.480848538528387 8.175933449971485 4 1.9025624061323108 4.628654143876086 5 0.9656927151183936 2.9367351979733507 6 0.5756711833907759 2.1007848151971165 7 0.3716671216484383 1.5823699002604923 8 0.23712227910691008 1.1537671681684782 9 0.18103286498396967 0.9909591745669972 >10 0.8396308168914646 8.825455621556618 >50 0.03951493872404321 1.6115958522234513 >100 0.01735832923091218 1.8783645149462445 >500 9.452652340436171E-4 0.3653334664442928 >1k 6.193091237118585E-4 0.7715233250913697 >5k 3.259521703670694E-5 0.14503853447849338 >10k+ 9.778565111012083E-5 1.0673694226793444 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22288 0.3746504461581184 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19424 0.3265079983029115 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12128 0.20386578477232858 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7316 0.12297840380890138 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.680951391592419E-5 2 0.0 0.0 0.0 0.0 1.680951391592419E-5 3 0.0 0.0 0.0 0.0 1.680951391592419E-5 4 0.0 0.0 0.0 0.0 1.680951391592419E-5 5 0.0 0.0 0.0 0.0 1.680951391592419E-5 6 0.0 0.0 0.0 0.0 1.680951391592419E-5 7 0.0 0.0 0.0 0.0 1.680951391592419E-5 8 0.0 0.0 0.0 0.0 3.361902783184838E-5 9 0.0 0.0 0.0 6.723805566369676E-5 5.042854174777257E-5 10-11 1.680951391592419E-5 0.0 0.0 1.2607135436943143E-4 5.042854174777257E-5 12-13 1.680951391592419E-5 0.0 0.0 1.76499896117204E-4 9.245232653758305E-5 14-15 2.521427087388629E-5 0.0 0.0 1.9330941003312818E-4 1.0085708349554514E-4 16-17 3.361902783184838E-5 0.0 0.0 2.1011892394905238E-4 1.4288086828535563E-4 18-19 6.723805566369676E-5 0.0 0.0 3.1938076440255966E-4 1.512856252433177E-4 20-21 6.723805566369676E-5 0.0 0.0 8.993089945019443E-4 1.512856252433177E-4 22-23 6.723805566369676E-5 0.0 0.0 0.0026474984417580597 1.9330941003312818E-4 24-25 6.723805566369676E-5 8.404756957962096E-6 0.0 0.007144043414267781 2.1852368090701448E-4 26-27 6.723805566369676E-5 1.680951391592419E-5 0.0 0.015943823949254095 2.1852368090701448E-4 28-29 6.723805566369676E-5 2.521427087388629E-5 0.0 0.04369633142444493 2.3533319482293866E-4 30-31 6.723805566369676E-5 3.361902783184838E-5 0.0 0.1293828286108685 2.3533319482293866E-4 32-33 6.723805566369676E-5 3.361902783184838E-5 0.0 0.32159121548250363 2.6895222265478706E-4 34-35 6.723805566369676E-5 3.361902783184838E-5 0.0 0.6238178709338627 2.8576173657071126E-4 36-37 6.723805566369676E-5 3.361902783184838E-5 0.0 1.0749600101663939 2.8576173657071126E-4 38-39 6.723805566369676E-5 4.2023784789810476E-5 0.0 1.697794524536175 2.8576173657071126E-4 40-41 6.723805566369676E-5 6.723805566369676E-5 0.0 2.370956723570233 2.8576173657071126E-4 42-43 6.723805566369676E-5 6.723805566369676E-5 0.0 2.991412691720911 2.8576173657071126E-4 44-45 6.723805566369676E-5 6.723805566369676E-5 0.0 3.704959747937977 2.8576173657071126E-4 46-47 6.723805566369676E-5 6.723805566369676E-5 0.0 4.530348904994645 3.025712504866354E-4 48-49 6.723805566369676E-5 6.723805566369676E-5 0.0 5.3689923637740185 3.193807644025596E-4 50-51 6.723805566369676E-5 6.723805566369676E-5 0.0 6.200727112333947 3.361902783184838E-4 52-53 6.723805566369676E-5 6.723805566369676E-5 0.0 6.98910171974775 3.445950352764459E-4 54-55 6.723805566369676E-5 6.723805566369676E-5 0.0 7.8272492978666035 3.698093061503322E-4 56-57 6.723805566369676E-5 6.723805566369676E-5 0.0 8.744460424689008 3.8661882006625636E-4 58-59 6.723805566369676E-5 7.564281262165885E-5 0.0 9.750946879918885 4.0342833398218056E-4 60-61 6.723805566369676E-5 8.404756957962095E-5 0.0 10.72889078051952 4.0342833398218056E-4 62-63 6.723805566369676E-5 8.404756957962095E-5 0.0 11.70975953654153 4.2023784789810476E-4 64-65 6.723805566369676E-5 8.404756957962095E-5 0.0 12.642015178318687 4.2023784789810476E-4 66-67 6.723805566369676E-5 8.404756957962095E-5 0.0 13.64104661412685 4.2023784789810476E-4 68-69 6.723805566369676E-5 8.404756957962095E-5 0.0 14.727218570075166 4.2023784789810476E-4 70-71 6.723805566369676E-5 8.404756957962095E-5 0.0 15.824190638714462 4.2864260485606684E-4 72-73 6.723805566369676E-5 8.404756957962095E-5 0.0 16.854159984884888 4.3704736181402896E-4 74-75 6.723805566369676E-5 8.404756957962095E-5 0.0 17.833036813507857 4.3704736181402896E-4 76-77 6.723805566369676E-5 8.404756957962095E-5 0.0 18.86770441881778 4.3704736181402896E-4 78-79 6.723805566369676E-5 8.404756957962095E-5 0.0 19.944858070550204 4.5385687572995317E-4 80-81 6.723805566369676E-5 8.404756957962095E-5 0.0 21.013211941747638 4.706663896458773E-4 82-83 6.723805566369676E-5 8.404756957962095E-5 0.0 22.06462182291782 5.042854174777257E-4 84-85 6.723805566369676E-5 8.404756957962095E-5 0.0 23.057980047779363 5.042854174777257E-4 86-87 6.723805566369676E-5 8.404756957962095E-5 0.0 24.039294255920144 5.126901744356878E-4 88 6.723805566369676E-5 8.404756957962095E-5 0.0 24.800655974471056 5.210949313936499E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10625 0.0 53.06148 1 TACCTGG 5280 0.0 44.770996 2 GTATCAA 18825 0.0 40.08936 1 ACCTGGG 6150 0.0 37.21612 3 ATCAACG 23825 0.0 31.246367 3 CAACGCA 23905 0.0 31.155697 5 TATCAAC 24360 0.0 30.96424 2 AACGCAG 25575 0.0 29.54337 6 TCAACGC 25560 0.0 29.217808 4 GTACATG 22645 0.0 27.637249 1 TACATGG 23080 0.0 26.776426 2 GTACTAG 2495 0.0 26.57282 1 CCTGGGG 6140 0.0 26.561077 4 GTGGTAT 4275 0.0 26.507576 1 TGGGGGG 13120 0.0 26.395102 6 ACATGGG 22740 0.0 26.164986 3 ACGCAGA 28695 0.0 25.905376 7 CTGGGGG 4320 0.0 25.56119 5 CGCAGAG 29225 0.0 25.355186 8 ATGGGGG 13020 0.0 24.793777 5 >>END_MODULE