FastQCFastQC Report
Mon 6 Jun 2016
ERR1211451_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211451_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504900
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111180.44385005389436705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92550.36947582737833845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52540.20974889217134415No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36050.14391792087508481No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33720.13461615234141083No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28120.11225997045790251No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54550.045.0701941
TACCTGG20700.043.649542
GTATCAA93500.038.334231
ACCTGGG24750.035.3250773
ATCAACG109650.032.3656043
CAACGCA111300.031.8435555
TATCAAC112350.031.6663132
TCAACGC114200.031.0754594
AACGCAG119000.029.8243796
GTACATG71200.028.5770531
ACGCAGA127600.027.4464197
TACATGG73050.026.9924682
CGCAGAG129800.026.8709668
ACATGGG73950.025.4889283
GTACCTG37300.024.7491861
GTACTAG7850.024.599591
GCAGAGT137450.024.4847859
CCTGGGG25450.024.3793894
CATGGGG52750.023.79174
CCGTATC8950.023.63103994
Produced by FastQC (version 0.11.5)