##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211451_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2504900 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.426028184757875 31.0 31.0 34.0 30.0 34.0 2 31.47432592119446 31.0 31.0 34.0 28.0 34.0 3 31.54041279092978 31.0 31.0 34.0 28.0 34.0 4 35.16606611042357 37.0 35.0 37.0 32.0 37.0 5 35.22162880753723 37.0 35.0 37.0 32.0 37.0 6 35.28783344644497 37.0 35.0 37.0 32.0 37.0 7 35.23161044353068 37.0 35.0 37.0 32.0 37.0 8 35.239680625973094 37.0 35.0 37.0 32.0 37.0 9 36.81667930855523 39.0 37.0 39.0 32.0 39.0 10-11 36.74872849215538 39.0 37.0 39.0 32.0 39.0 12-13 36.67071280290631 39.0 37.0 39.0 32.0 39.0 14-15 37.869686813844865 40.0 37.5 41.0 32.0 41.0 16-17 37.860416583496345 40.0 37.5 41.0 32.0 41.0 18-19 37.86196015809014 40.0 37.5 41.0 32.0 41.0 20-21 37.84720348117689 40.0 37.5 41.0 32.0 41.0 22-23 37.712646812247996 40.0 37.0 41.0 32.0 41.0 24-25 37.61929278613917 40.0 37.0 41.0 32.0 41.0 26-27 37.479811968541654 40.0 37.0 41.0 32.0 41.0 28-29 37.35385983472394 40.0 36.5 41.0 31.0 41.0 30-31 37.1858469399976 39.5 36.0 41.0 31.0 41.0 32-33 36.97819633518304 39.0 36.0 41.0 30.5 41.0 34-35 36.83635055291629 39.0 35.0 41.0 30.0 41.0 36-37 36.686532396502855 39.0 35.0 41.0 30.0 41.0 38-39 36.528461016407846 39.0 35.0 41.0 30.0 41.0 40-41 36.36779132899517 39.0 35.0 40.0 29.5 41.0 42-43 36.1642017645415 39.0 35.0 40.0 29.0 41.0 44-45 36.01772785340732 39.0 35.0 40.0 28.0 41.0 46-47 35.77228452233622 38.0 35.0 40.0 27.0 41.0 48-49 35.5172118647451 38.0 34.0 40.0 26.0 41.0 50-51 34.84403848457025 37.5 33.5 39.5 25.0 40.5 52-53 35.13984650085832 38.0 34.0 40.0 26.0 40.5 54-55 35.544585212982554 38.0 35.0 40.0 27.0 41.0 56-57 35.457172342209276 38.0 34.0 40.0 26.0 41.0 58-59 35.25751207633039 38.0 34.0 40.0 26.0 41.0 60-61 35.02829194778235 38.0 34.0 40.0 26.0 41.0 62-63 34.748409317737234 37.0 34.0 40.0 26.0 41.0 64-65 34.535161882709886 37.0 34.0 40.0 25.5 41.0 66-67 34.32672501896283 36.5 33.0 40.0 25.0 41.0 68-69 33.98103616910855 36.0 33.0 39.0 24.5 41.0 70-71 33.596685496427 35.5 33.0 39.0 24.0 41.0 72-73 33.20515968701345 35.0 32.0 38.5 23.0 40.0 74-75 32.82548704539103 35.0 32.0 37.0 22.5 40.0 76-77 32.43415685256896 35.0 32.0 37.0 21.5 39.0 78-79 32.03174577827458 35.0 31.0 36.0 20.0 39.0 80-81 31.62888438660226 35.0 31.0 36.0 20.0 38.5 82-83 31.264807776757557 35.0 31.0 35.5 19.0 37.0 84-85 30.934369835123157 34.0 30.0 35.0 18.0 37.0 86-87 30.59984949498982 34.0 30.0 35.0 17.5 36.0 88-89 30.277864585412594 34.0 29.0 35.0 15.5 36.0 90-91 29.965462493512714 34.0 29.0 35.0 13.5 36.0 92-93 29.67033115094415 34.0 29.0 35.0 10.5 35.0 94-95 29.383899756477305 34.0 28.5 35.0 10.0 35.0 96-97 29.08519881033175 34.0 27.0 35.0 10.0 35.0 98-99 28.758740269072618 34.0 26.5 35.0 10.0 35.0 100 27.499660265878877 32.0 24.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3130720249421337 1101 2 -0.33041808934762784 1101 3 -0.5287859635063619 1101 4 -0.31901493998879005 1101 5 -0.2676202671788417 1101 6 -0.4643741139669828 1101 7 -0.46453260579532696 1101 8 -0.5031095582808405 1101 9 -0.525527793053449 1101 10-11 -0.595283012053244 1101 12-13 -0.7158806860015972 1101 14-15 -0.9791578507888019 1101 16-17 -0.8686755438515874 1101 18-19 -0.808826536989244 1101 20-21 -0.8746608446406086 1101 22-23 -0.7001880388979416 1101 24-25 -0.9236658418356214 1101 26-27 -0.9962628871061128 1101 28-29 -1.0856558497328663 1101 30-31 -1.1376437515205424 1101 32-33 -1.0238180262564427 1101 34-35 -1.0565391925792156 1101 36-37 -0.9518376556340584 1101 38-39 -1.0100185107214443 1101 40-41 -1.2429463888287628 1101 42-43 -1.2668090150978983 1101 44-45 -1.3801699778748215 1101 46-47 -1.4379394121571138 1101 48-49 -1.4778294826557001 1101 50-51 -1.4274168600242731 1101 52-53 -1.2504194426537438 1101 54-55 -1.2373565443109271 1101 56-57 -1.243198281350793 1101 58-59 -1.4001929403974671 1101 60-61 -1.4373878359728707 1101 62-63 -1.4248303573656287 1101 64-65 -1.348060622652639 1101 66-67 -1.1696006998572912 1101 68-69 -1.2006248961792423 1101 70-71 -1.3676540052941917 1101 72-73 -1.2708883954961259 1101 74-75 -1.3039466512633524 1101 76-77 -1.2837669777306608 1101 78-79 -1.5230280897000803 1101 80-81 -1.392549948041598 1101 82-83 -1.5992786268111168 1101 84-85 -1.4706556549920613 1101 86-87 -1.5481204541865772 1101 88-89 -1.6350738258556312 1101 90-91 -1.8362077875884033 1101 92-93 -2.006574788215321 1101 94-95 -2.0826984258411727 1101 96-97 -1.8154413270875587 1101 98-99 -2.0281372553034345 1101 100 -2.1005029673755544 1102 1 -0.25954047448119866 1102 2 -0.3076880247359206 1102 3 -0.39826032417315815 1102 4 -0.25292820188988685 1102 5 -0.19908033773101153 1102 6 -0.247692962837192 1102 7 -0.3133006013656541 1102 8 -0.20988122813775334 1102 9 -0.358685571026804 1102 10-11 -0.28470014075595884 1102 12-13 -0.43355168517285847 1102 14-15 -0.6298684603830154 1102 16-17 -0.6944952911252074 1102 18-19 -0.5957658140861923 1102 20-21 -0.7920450881115997 1102 22-23 -0.6981499007564977 1102 24-25 -0.6178371746995808 1102 26-27 -0.6526396261584253 1102 28-29 -0.631964878503787 1102 30-31 -0.7112007598005192 1102 32-33 -0.6977019433686493 1102 34-35 -0.8084556337282933 1102 36-37 -0.9056326766456948 1102 38-39 -0.7003672745916916 1102 40-41 -0.8913924259529935 1102 42-43 -0.8554216143806457 1102 44-45 -0.7660748076182173 1102 46-47 -0.9083539794933628 1102 48-49 -0.9372028554454559 1102 50-51 -0.7676041863023926 1102 52-53 -0.5259758110504791 1102 54-55 -0.7195250701226072 1102 56-57 -0.7727397105822789 1102 58-59 -0.7174329351170528 1102 60-61 -0.6447129865012755 1102 62-63 -0.7167218540690428 1102 64-65 -0.8195150002607647 1102 66-67 -0.7584417961294676 1102 68-69 -0.6725378720580366 1102 70-71 -0.8422356059923786 1102 72-73 -0.9065741248163519 1102 74-75 -1.0463695810852123 1102 76-77 -0.8686748088748217 1102 78-79 -1.0351899966621545 1102 80-81 -1.1492751392731826 1102 82-83 -1.080850814441753 1102 84-85 -1.1721492048222828 1102 86-87 -1.2479897731601248 1102 88-89 -1.188046624400581 1102 90-91 -1.202870283096388 1102 92-93 -1.2730730959166543 1102 94-95 -1.34333740151461 1102 96-97 -1.627666534820431 1102 98-99 -1.7272573700057166 1102 100 -1.4665950225558575 1103 1 -0.5260947967081577 1103 2 -0.6133687558995575 1103 3 -0.6325116087243252 1103 4 -0.46333203799594713 1103 5 -0.4579111620579752 1103 6 -0.5598202985936211 1103 7 -0.5182695454650315 1103 8 -0.5493195526156853 1103 9 -0.5538253247675584 1103 10-11 -0.6682332931596022 1103 12-13 -0.7547737132015016 1103 14-15 -1.04768716078744 1103 16-17 -1.0061845973406705 1103 18-19 -0.9800918831014513 1103 20-21 -0.8797332635894648 1103 22-23 -0.9476788330233958 1103 24-25 -0.963562820300865 1103 26-27 -0.9948371866708285 1103 28-29 -0.9663120394509761 1103 30-31 -1.019992988517572 1103 32-33 -0.9997651018898281 1103 34-35 -0.9787226610874171 1103 36-37 -1.103036749926595 1103 38-39 -1.0333666523359426 1103 40-41 -1.040872960550324 1103 42-43 -1.137827048572511 1103 44-45 -1.1956618871032916 1103 46-47 -1.3137555707527255 1103 48-49 -1.161412279950774 1103 50-51 -1.125486136247126 1103 52-53 -1.0316365005028487 1103 54-55 -0.9441911218267194 1103 56-57 -0.8464282256315059 1103 58-59 -0.8369574564570996 1103 60-61 -0.992012869139117 1103 62-63 -0.9198233745260254 1103 64-65 -0.8104301538074026 1103 66-67 -0.9496690402541432 1103 68-69 -0.9172775792390055 1103 70-71 -0.9288475240716636 1103 72-73 -0.8876902818488404 1103 74-75 -0.9261228142333238 1103 76-77 -0.9745118916418392 1103 78-79 -1.037899566010413 1103 80-81 -1.0533757696681398 1103 82-83 -1.0731386939324281 1103 84-85 -1.2024948937101598 1103 86-87 -1.1143491919329946 1103 88-89 -1.1313502423232968 1103 90-91 -1.0231939348537118 1103 92-93 -1.0839012211764718 1103 94-95 -1.1801276383131167 1103 96-97 -1.521892027702119 1103 98-99 -1.4325046225846592 1103 100 -1.5178759854681907 1104 1 -0.39058197367196 1104 2 -0.4125598122500591 1104 3 -0.48329004476660486 1104 4 -0.199027906675461 1104 5 -0.2711554000566778 1104 6 -0.29869486312212956 1104 7 -0.2831641466026653 1104 8 -0.4044379418140238 1104 9 -0.38241193684856967 1104 10-11 -0.4664648360572414 1104 12-13 -0.5092957278845205 1104 14-15 -0.7233617725535382 1104 16-17 -0.6447826251714872 1104 18-19 -0.7207821423176952 1104 20-21 -0.7681492298933179 1104 22-23 -0.6264238878386763 1104 24-25 -0.7441442362474859 1104 26-27 -0.6528419891586736 1104 28-29 -0.7596722063571164 1104 30-31 -0.8847458768991032 1104 32-33 -0.77961827172863 1104 34-35 -0.7739674108266286 1104 36-37 -0.9282232399771786 1104 38-39 -0.9428623911120937 1104 40-41 -0.9527305785950801 1104 42-43 -0.9414121844789776 1104 44-45 -0.9314955320734768 1104 46-47 -0.9027984191791276 1104 48-49 -0.743462981625747 1104 50-51 -0.8328050096307109 1104 52-53 -0.8159132733884746 1104 54-55 -0.7558410913191125 1104 56-57 -0.6546091849481357 1104 58-59 -0.7008590841855593 1104 60-61 -0.7155352184216923 1104 62-63 -0.8320598773701207 1104 64-65 -0.7616179822562401 1104 66-67 -0.6918502357587002 1104 68-69 -0.7814350772215661 1104 70-71 -0.7851370117406873 1104 72-73 -0.8403247613496987 1104 74-75 -0.8256297719360077 1104 76-77 -0.8936519707647932 1104 78-79 -0.7463009673921128 1104 80-81 -0.8024999061060321 1104 82-83 -0.8927944500290295 1104 84-85 -0.943800225652609 1104 86-87 -1.1173458262550362 1104 88-89 -1.0247999638183671 1104 90-91 -1.007964734153024 1104 92-93 -1.0475184392763417 1104 94-95 -0.9861041769790475 1104 96-97 -1.0275408556195345 1104 98-99 -1.0713585206695697 1104 100 -0.8590199223272847 1105 1 -0.39482289427139605 1105 2 -0.3886476559225045 1105 3 -0.49436245108115173 1105 4 -0.30711484417832224 1105 5 -0.39231039066044815 1105 6 -0.36440878663687926 1105 7 -0.4478851179726391 1105 8 -0.36895940719509923 1105 9 -0.5304879111080325 1105 10-11 -0.4514364478300905 1105 12-13 -0.4956051722765693 1105 14-15 -0.5887132282351573 1105 16-17 -0.5497104309225378 1105 18-19 -0.5348196912307017 1105 20-21 -0.7289181711265016 1105 22-23 -0.7695298192976665 1105 24-25 -0.7179143242781834 1105 26-27 -0.83567608148077 1105 28-29 -0.7168081548791676 1105 30-31 -0.730771485971438 1105 32-33 -0.788490888778469 1105 34-35 -0.9012914756154018 1105 36-37 -0.7584985770225714 1105 38-39 -0.8464430717017919 1105 40-41 -0.7667469992017502 1105 42-43 -0.7627619858924604 1105 44-45 -0.8064941137380899 1105 46-47 -0.9549152015884843 1105 48-49 -0.8676637740937352 1105 50-51 -0.7447375336455835 1105 52-53 -0.7502598981219819 1105 54-55 -0.7202957482130259 1105 56-57 -0.6073957346683159 1105 58-59 -0.6131258462687086 1105 60-61 -0.7539076462949339 1105 62-63 -0.5963053444680639 1105 64-65 -0.8010739205428052 1105 66-67 -0.7128376867185295 1105 68-69 -0.7196915146056782 1105 70-71 -0.6452372029518685 1105 72-73 -0.635710417499034 1105 74-75 -0.6014446496285402 1105 76-77 -0.654975177900841 1105 78-79 -0.7466890043885606 1105 80-81 -0.7456550782567355 1105 82-83 -0.8091826990910711 1105 84-85 -0.7348245494196455 1105 86-87 -0.7888804807857106 1105 88-89 -0.877693739693207 1105 90-91 -0.9542382878128564 1105 92-93 -0.7941232378277228 1105 94-95 -0.8918833752224238 1105 96-97 -1.0497630408889584 1105 98-99 -1.020444780110303 1105 100 -0.9080193992060686 1106 1 -0.4124931141888055 1106 2 -0.5316131797869055 1106 3 -0.47400808634476377 1106 4 -0.3869189352183682 1106 5 -0.2808631612356933 1106 6 -0.3072830783875702 1106 7 -0.5311272191922711 1106 8 -0.50815080256627 1106 9 -0.6788745432025252 1106 10-11 -0.6764460512104762 1106 12-13 -0.6995233514756549 1106 14-15 -0.8185101167399083 1106 16-17 -0.7791207833665865 1106 18-19 -0.7063456683746594 1106 20-21 -0.7615384534658816 1106 22-23 -0.7708959001581306 1106 24-25 -0.7142554363496316 1106 26-27 -0.7356064569729384 1106 28-29 -0.6777896203550213 1106 30-31 -0.812719531683527 1106 32-33 -0.8436214947517726 1106 34-35 -0.899361196157038 1106 36-37 -0.872094562809572 1106 38-39 -0.9722377514290557 1106 40-41 -0.9022362914326223 1106 42-43 -0.8316830461056597 1106 44-45 -0.9787475469465861 1106 46-47 -0.9770245840911187 1106 48-49 -1.1335117525821587 1106 50-51 -0.9068618013878265 1106 52-53 -0.6790196215446187 1106 54-55 -0.8581426703296131 1106 56-57 -1.0081928152406832 1106 58-59 -0.9996811137228505 1106 60-61 -0.9510866533054028 1106 62-63 -0.8528404448233928 1106 64-65 -0.8296559814282318 1106 66-67 -0.8725971220397568 1106 68-69 -0.8940456872744562 1106 70-71 -0.9968529717024595 1106 72-73 -0.988492996967004 1106 74-75 -1.1020496625931067 1106 76-77 -0.9548239246596992 1106 78-79 -0.939284798107952 1106 80-81 -0.9645833410052198 1106 82-83 -0.8674584121420637 1106 84-85 -0.9149311469419708 1106 86-87 -0.9700482518411206 1106 88-89 -0.9768150303875309 1106 90-91 -0.8962194651872899 1106 92-93 -0.928738622443106 1106 94-95 -0.9170442318439562 1106 96-97 -1.0623914861016708 1106 98-99 -1.3300553630354912 1106 100 -1.2640887356124857 1107 1 -0.5848941258755325 1107 2 -0.6491868759320276 1107 3 -0.6721177148115345 1107 4 -0.5865853402419106 1107 5 -0.5307214378796843 1107 6 -0.4654419829750509 1107 7 -0.5930625061275592 1107 8 -0.5939871884026573 1107 9 -0.6255712988278574 1107 10-11 -0.5579718666648645 1107 12-13 -0.6202520275614773 1107 14-15 -0.7758622883777946 1107 16-17 -0.8687052068795893 1107 18-19 -0.841188900539322 1107 20-21 -0.954464277641712 1107 22-23 -0.9143897079376515 1107 24-25 -0.8648539449838566 1107 26-27 -0.9246316183195304 1107 28-29 -0.8990012460309273 1107 30-31 -1.062106850713704 1107 32-33 -1.1154174567263055 1107 34-35 -0.9850068005526396 1107 36-37 -0.8425343412850665 1107 38-39 -1.1078026825297016 1107 40-41 -0.9452482398017068 1107 42-43 -0.8773037944049733 1107 44-45 -1.0516173358877978 1107 46-47 -0.9487169789978012 1107 48-49 -1.1256799018931716 1107 50-51 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2311 56-57 1.3176814688820784 2311 58-59 1.3479184127972488 2311 60-61 1.2793827358707048 2311 62-63 1.1382809366238575 2311 64-65 1.2168455765295363 2311 66-67 1.1905249731088148 2311 68-69 1.1319861333602148 2311 70-71 1.1370291283201723 2311 72-73 1.0798478215265632 2311 74-75 1.048462113478756 2311 76-77 1.0926707225803582 2311 78-79 1.175081487337767 2311 80-81 1.1304137867821247 2311 82-83 1.2089803052844346 2311 84-85 1.2594434940314478 2311 86-87 1.337087020670932 2311 88-89 1.3976666657845946 2311 90-91 1.378022879930814 2311 92-93 1.4198957709978046 2311 94-95 1.4803437953811382 2311 96-97 1.5781032307677911 2311 98-99 1.842473663719602 2311 100 1.9374317963561118 2312 1 0.6576809529364844 2312 2 0.681394950294397 2312 3 0.738848907609789 2312 4 0.5861892574020189 2312 5 0.6534186815827354 2312 6 0.4693168799197025 2312 7 0.5267110130362056 2312 8 0.6316709610359794 2312 9 0.768900107620901 2312 10-11 0.8119399191876937 2312 12-13 0.911165620798684 2312 14-15 1.0973748113401385 2312 16-17 1.117955514319462 2312 18-19 1.0403632121756843 2312 20-21 1.1080962140346386 2312 22-23 1.1734234661124603 2312 24-25 1.178667223646194 2312 26-27 1.1793846833230788 2312 28-29 1.2098198230471127 2312 30-31 1.0753198679412392 2312 32-33 1.1933130476705784 2312 34-35 1.2487209385104663 2312 36-37 1.3033145470507534 2312 38-39 1.3720086525734416 2312 40-41 1.3578012320195398 2312 42-43 1.3546990677154795 2312 44-45 1.344973007566658 2312 46-47 1.3043283448227854 2312 48-49 1.293638028531035 2312 50-51 1.231529380600918 2312 52-53 1.2090640766000575 2312 54-55 1.1952709214986115 2312 56-57 1.1215367032083563 2312 58-59 1.0870762346198646 2312 60-61 1.158036236000413 2312 62-63 1.1593833408760048 2312 64-65 1.1762331159242763 2312 66-67 1.0763657714353272 2312 68-69 1.1143606083269617 2312 70-71 1.157449196319213 2312 72-73 1.1851780474357483 2312 74-75 1.18224520920176 2312 76-77 1.1253407616025441 2312 78-79 1.1916824731032243 2312 80-81 1.0185154707521313 2312 82-83 1.140499686669692 2312 84-85 1.2996144023596763 2312 86-87 1.251757043134905 2312 88-89 1.2749516988182243 2312 90-91 1.3650688400034525 2312 92-93 1.353234485749514 2312 94-95 1.4419301271304015 2312 96-97 1.358561713814943 2312 98-99 1.2438502029432428 2312 100 1.4914094924467207 2313 1 0.6032948368603996 2313 2 0.5912662885739977 2313 3 0.6221811499857814 2313 4 0.532332478282143 2313 5 0.4855223501383179 2313 6 0.503819185179367 2313 7 0.6126653157274404 2313 8 0.5463818083580634 2313 9 0.7299481435859789 2313 10-11 0.7033970663832179 2313 12-13 0.8270032712060811 2313 14-15 0.9443283018247683 2313 16-17 0.9568058634703434 2313 18-19 0.9626802029680022 2313 20-21 1.0371700157404504 2313 22-23 1.1286495748145526 2313 24-25 1.126458012760132 2313 26-27 1.1196409091271988 2313 28-29 1.071219314260226 2313 30-31 1.1349620606497268 2313 32-33 1.0888556631361652 2313 34-35 1.1735181317666559 2313 36-37 1.2498389907293088 2313 38-39 1.1986969424599607 2313 40-41 1.2496049808161516 2313 42-43 1.2328119697777922 2313 44-45 1.1893571265228644 2313 46-47 1.1338824015365319 2313 48-49 1.1608008915415269 2313 50-51 1.112686777247184 2313 52-53 0.9582127402441003 2313 54-55 1.011559827486586 2313 56-57 0.950616249138541 2313 58-59 0.9924801025757333 2313 60-61 1.0379629659693208 2313 62-63 0.9289232218236165 2313 64-65 1.0412439542515273 2313 66-67 0.9942108946778845 2313 68-69 1.0457245855388706 2313 70-71 1.052198585605744 2313 72-73 1.0878266914870878 2313 74-75 1.1041903839196934 2313 76-77 1.0743154620790918 2313 78-79 1.091944926269985 2313 80-81 1.1853534622506707 2313 82-83 1.1523468154926064 2313 84-85 1.2879624213147203 2313 86-87 1.291697214143582 2313 88-89 1.3632875168625667 2313 90-91 1.4092250045358519 2313 92-93 1.6267607582288015 2313 94-95 1.6124216011874388 2313 96-97 1.6356453421520492 2313 98-99 1.7111484225899147 2313 100 1.6423934246488407 2314 1 0.43956116185754013 2314 2 0.4663525982846224 2314 3 0.4997653642627924 2314 4 0.4199175418097809 2314 5 0.44227703117086037 2314 6 0.5224436207046281 2314 7 0.48619897374528875 2314 8 0.5949940220590548 2314 9 0.7027979702280831 2314 10-11 0.6534112821002651 2314 12-13 0.44313090988316617 2314 14-15 0.8378590428513277 2314 16-17 0.8302656736074354 2314 18-19 0.9526987387860757 2314 20-21 0.9184457009564753 2314 22-23 0.7077821945311982 2314 24-25 0.665137686500195 2314 26-27 0.8735363848367896 2314 28-29 0.9176674158158349 2314 30-31 0.877508040186477 2314 32-33 0.9200606390063726 2314 34-35 0.9753418051817846 2314 36-37 0.9116295433699193 2314 38-39 0.9746332412780276 2314 40-41 1.0349587306269115 2314 42-43 1.1106555747359366 2314 44-45 1.093284726060574 2314 46-47 0.9756227006943021 2314 48-49 1.1070553270298618 2314 50-51 0.9954246505020663 2314 52-53 0.8760302030524656 2314 54-55 0.8720704117341 2314 56-57 0.8287220140733567 2314 58-59 0.8422735131293351 2314 60-61 0.8459067825016433 2314 62-63 0.8888616696150109 2314 64-65 0.8084385101857521 2314 66-67 0.9521554496890161 2314 68-69 0.8745811988012093 2314 70-71 0.8990583890965382 2314 72-73 0.876377131435838 2314 74-75 0.8516795559741794 2314 76-77 0.8791521290288458 2314 78-79 1.0141147985500787 2314 80-81 1.0197117426289246 2314 82-83 0.8766172907406471 2314 84-85 0.947302147412934 2314 86-87 1.2117550370342123 2314 88-89 1.307813911941345 2314 90-91 1.3834631713360572 2314 92-93 1.4304306286626947 2314 94-95 1.4117289080659994 2314 96-97 1.6386934134300226 2314 98-99 1.5649942355996949 2314 100 1.4826715357677465 2315 1 0.5576535808862566 2315 2 0.46103247446333384 2315 3 0.5896916173262845 2315 4 0.47615749805864027 2315 5 0.44119987601359156 2315 6 -0.3319775656895132 2315 7 0.2679519569642679 2315 8 0.3786784776727501 2315 9 0.6474215898271325 2315 10-11 0.7119965075042956 2315 12-13 0.6970162121126222 2315 14-15 0.876463923864506 2315 16-17 0.7781392758991998 2315 18-19 0.8568593373804916 2315 20-21 0.8710363022464023 2315 22-23 0.7759589721265385 2315 24-25 0.9038634870701472 2315 26-27 0.965143549149559 2315 28-29 1.01252703529115 2315 30-31 1.0173707139511308 2315 32-33 1.0870920212769306 2315 34-35 1.1220038762109041 2315 36-37 1.2055356627432943 2315 38-39 1.2463146449170353 2315 40-41 1.2806819364712823 2315 42-43 1.3386369156899534 2315 44-45 1.3409307769581034 2315 46-47 1.3860145019422347 2315 48-49 1.324604844288011 2315 50-51 1.3219653727115528 2315 52-53 1.2668923859003343 2315 54-55 1.0659787510697427 2315 56-57 1.072336413569353 2315 58-59 0.9104148870608313 2315 60-61 0.8699643348659265 2315 62-63 1.0308516669489478 2315 64-65 1.1070526855812943 2315 66-67 1.1479640662148327 2315 68-69 1.1493395648500524 2315 70-71 1.0865997503605769 2315 72-73 1.2167092903986045 2315 74-75 1.2090479790202906 2315 76-77 1.183521898571506 2315 78-79 1.1757018548443163 2315 80-81 1.12873456363074 2315 82-83 1.3052774877428348 2315 84-85 1.308949047998393 2315 86-87 1.3604687319709932 2315 88-89 1.5919443064233825 2315 90-91 1.7045715616782324 2315 92-93 1.87310057624423 2315 94-95 1.8135474464554555 2315 96-97 1.7239761577158284 2315 98-99 1.859765982444877 2315 100 1.8964003296043757 2316 1 -0.10598098167048065 2316 2 -0.0784312052427012 2316 3 0.039435590721204505 2316 4 -0.015703566479778885 2316 5 0.03736869290947453 2316 6 0.13232291245680727 2316 7 0.149370782977563 2316 8 0.17947588103564982 2316 9 0.33326723658630897 2316 10-11 0.09312673066880706 2316 12-13 0.2923516981393348 2316 14-15 0.37728054770401087 2316 16-17 0.3741485135559586 2316 18-19 0.3088854713146816 2316 20-21 0.35322498227278487 2316 22-23 0.2345293333630778 2316 24-25 0.3516928052952082 2316 26-27 0.41695830712433235 2316 28-29 0.5307018194057704 2316 30-31 0.4888674863895588 2316 32-33 0.4421098963201828 2316 34-35 0.5400807344613625 2316 36-37 0.6032878033622708 2316 38-39 0.5294041738970776 2316 40-41 0.5208790005681223 2316 42-43 0.530520256267927 2316 44-45 0.4743001363642563 2316 46-47 0.5434280369033004 2316 48-49 0.5389664829765692 2316 50-51 0.23302335645399097 2316 52-53 0.08221519626914642 2316 54-55 -0.031769989003372245 2316 56-57 -0.006544274582850562 2316 58-59 0.08838615096980362 2316 60-61 0.16529489256529928 2316 62-63 0.22938415891824349 2316 64-65 0.2377337409924536 2316 66-67 0.1950955825724492 2316 68-69 0.25940222360379295 2316 70-71 0.29876966331645605 2316 72-73 0.34287624438675124 2316 74-75 0.3574645074473821 2316 76-77 0.4934197047758815 2316 78-79 0.5695466697018468 2316 80-81 0.6048736725694361 2316 82-83 0.5204375154597471 2316 84-85 0.6121784823348406 2316 86-87 0.6783706838843138 2316 88-89 0.7570938368030689 2316 90-91 0.795605307906829 2316 92-93 0.8270841925128352 2316 94-95 0.8598695836147492 2316 96-97 0.9629028961586563 2316 98-99 1.0420735830098593 2316 100 1.1233540417683727 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 10.0 1 124.0 2 62.0 3 38.0 4 53.0 5 22.0 6 66.0 7 421.0 8 1306.0 9 1895.0 10 2317.0 11 2705.0 12 3266.0 13 3803.0 14 4552.0 15 5851.0 16 7722.0 17 10705.0 18 13485.0 19 16760.0 20 20492.0 21 24713.0 22 26784.0 23 27091.0 24 27023.0 25 28314.0 26 31044.0 27 36003.0 28 42368.0 29 50007.0 30 60927.0 31 74716.0 32 93489.0 33 121345.0 34 163826.0 35 228179.0 36 326888.0 37 473265.0 38 453765.0 39 119364.0 40 134.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.10489692361308 19.06321983720801 12.47391731082323 23.35796592835568 2 16.41032427479158 21.17737320946805 39.600041899687035 22.812260616053337 3 19.128978157568984 26.601964620852137 30.297128938226255 23.971928283352632 4 12.864963030017224 17.275405907131212 38.45165711054738 31.40797395230419 5 12.938924271399586 37.30728023387731 34.871750433967634 14.88204506075547 6 29.133662011830346 37.25042050103832 19.06588255688597 14.55003493024536 7 25.923095697566147 33.14184685848869 23.18881104355402 17.74624640039115 8 25.174499767333643 34.590796797392535 21.419085032064018 18.815618403209797 9 26.48243459826804 16.035969904200336 21.134435440164452 36.34716005736717 10-11 24.29361822114724 25.87136994092218 29.336001527268778 20.499010310661806 12-13 25.10618014470733 24.358061673445807 28.6452054540121 21.890552727834763 14-15 22.673366043606244 25.74019370614739 26.88052125608726 24.705918994159106 16-17 21.765420139815017 28.253645907368156 27.5156401480244 22.465293804792424 18-19 21.249707273921135 27.617190751992084 29.72459891286575 21.408503061221026 20-21 22.189963242465364 27.044149969782993 29.470294996634898 21.29559179111674 22-23 22.25219940646373 26.761661486268483 29.20802200364811 21.778117103619678 24-25 21.89670442176246 26.703591219564398 29.520369463812685 21.879334894860456 26-27 21.73983092857518 26.870948827175052 29.41135249752989 21.97786774671988 28-29 21.481273112512657 27.462880728204738 29.383693613854163 21.672152545428446 30-31 22.260218251017218 26.958039354338574 29.375187038218982 21.406555356425223 32-33 22.02029224863096 27.147361384750905 29.021972554472175 21.81037381214596 34-35 22.169785327519286 27.040306994425546 29.320882648905073 21.469025029150096 36-37 21.969071683304005 26.75418788511153 29.36031804407367 21.9164223875108 38-39 21.847726130166674 26.903862609874125 29.39321810151405 21.85519315844515 40-41 21.781024363636075 26.467532322328026 29.71346153385409 22.037981780181816 42-43 21.52686915211407 26.735973909051864 29.2837708080962 22.45338613073787 44-45 21.25784141545349 27.025349125825077 29.472140323271496 22.24466913544994 46-47 21.22461972015493 26.630312794800254 29.303574466793457 22.841493018251356 48-49 21.28975894772891 26.68402613105264 29.345595451926833 22.68061946929162 50-51 21.15061927763189 26.92711627788107 29.203784190450634 22.718480254036404 52-53 21.01864249562419 27.139284034710926 28.933580555330902 22.908492914333983 54-55 20.797302241023445 26.93853001131055 29.193272296809404 23.0708954508566 56-57 21.2280073935736 26.781754583839895 28.811971901864208 23.178266120722295 58-59 21.066346981846184 26.780912851138066 28.94200061029286 23.21073955672289 60-61 21.22509397783185 26.73853997541842 28.733796959965886 23.302569086783844 62-63 21.293991157094837 26.492716932196743 28.73544858925814 23.477843321450283 64-65 21.0238100895137 26.33177911102051 28.78408675479539 23.860324044670396 66-67 21.114573622267432 26.31663334496082 28.452969671823297 24.11582336094845 68-69 20.767153956146142 26.35837991007665 28.572198761072936 24.302267372704268 70-71 20.831403011302367 26.031151403810053 28.73856783417868 24.398877750708895 72-73 20.8386161294207 26.092283200339004 28.65398582596438 24.415114844275916 74-75 20.85097479076829 26.10755342551415 28.56346791753801 24.478003866179556 76-77 20.829740555344486 25.83917278295734 28.649535396158548 24.681551265539618 78-79 20.754723384749852 25.923160511730874 28.792637834473055 24.529478269046216 80-81 20.869704625378187 25.845349286190313 28.70452116073486 24.580424927696644 82-83 21.032601740561642 25.443680307009025 29.003045214234106 24.52067273819523 84-85 21.148956311847364 25.564944710809517 28.594645819188546 24.691453158154573 86-87 21.043541969859398 25.553053487689624 28.84254739788269 24.56085714456828 88-89 21.276338756390846 25.01760359167174 28.86933133902491 24.836726312912504 90-91 21.700348860563185 24.898310559770074 28.773649507933712 24.627691071733032 92-93 21.840591110188306 24.769968535880277 28.694019421507406 24.695420932424014 94-95 21.553027051093405 24.431635543966763 28.857940331465237 25.1573970734746 96-97 21.682438764741043 24.237067431958494 28.879730481404707 25.200763321895753 98-99 21.8673371734529 24.257764681429077 28.59204556549651 25.28285257962152 100 22.151317418355205 24.037274273802584 28.627632640347585 25.18377566749463 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2015.0 1 1377.0 2 1755.5 3 2622.0 4 1737.5 5 1020.5 6 1321.0 7 2168.5 8 3222.5 9 3713.0 10 3012.5 11 2258.0 12 2292.0 13 2468.5 14 2679.0 15 2938.5 16 3170.5 17 3438.5 18 3790.5 19 4125.5 20 4617.5 21 5247.0 22 5871.0 23 6767.0 24 7972.0 25 9451.0 26 11385.0 27 13997.5 28 16858.0 29 20523.0 30 25179.5 31 29642.5 32 35009.0 33 40779.0 34 46199.5 35 51533.0 36 57047.0 37 63608.5 38 68658.0 39 71101.5 40 72948.0 41 75663.0 42 78919.0 43 82925.0 44 87812.5 45 92960.0 46 98577.5 47 104177.5 48 108715.5 49 110500.5 50 109184.0 51 106686.5 52 102534.0 53 96766.0 54 90173.5 55 80687.5 56 70873.5 57 60794.5 58 50746.5 59 42500.0 60 34021.5 61 26445.5 62 20547.5 63 15811.0 64 11507.5 65 8015.5 66 5766.5 67 4276.5 68 3186.5 69 2342.5 70 1814.0 71 1364.0 72 1047.0 73 782.5 74 591.5 75 461.5 76 317.0 77 222.5 78 150.0 79 100.5 80 81.0 81 62.0 82 53.0 83 50.5 84 35.5 85 23.0 86 19.0 87 16.0 88 15.0 89 12.5 90 9.0 91 8.0 92 7.0 93 6.0 94 5.5 95 5.5 96 4.0 97 3.0 98 2.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22503892370952933 2 0.14723142640424766 3 0.023953052018044634 4 0.022795321170505808 5 0.027186714040480654 6 0.05345522775360294 7 0.0600023953052018 8 0.05249710567288115 9 0.041079484210946544 10-11 0.04327518064593397 12-13 0.040660305800630764 14-15 0.019481815641342968 16-17 0.01788494550680666 18-19 0.016986706056129983 20-21 0.02057966385883668 22-23 0.02347399097768374 24-25 0.020519781228791566 26-27 0.018184358657032218 28-29 0.017545610603217694 30-31 0.015908818715317977 32-33 0.02371352149786419 34-35 0.02475148708531279 36-37 0.02367359974450078 38-39 0.0222364166234181 40-41 0.02371352149786419 42-43 0.02940237135214979 44-45 0.03862429637909697 46-47 0.043355024152660786 48-49 0.03922312267954809 50-51 0.02762585332747814 52-53 0.04301568924907182 54-55 0.04167831051139766 56-57 0.044073615713202126 58-59 0.04726735598227474 60-61 0.023394147470956923 62-63 0.025250509002355388 64-65 0.025809413549443095 66-67 0.030999241486686094 68-69 0.03507126032975368 70-71 0.03992175336340772 72-73 0.043774202562976566 74-75 0.02471156533194938 76-77 0.01814443690366881 78-79 0.016747175535949538 80-81 0.02079923350233542 82-83 0.019102558984390595 84-85 0.01183679987225039 86-87 0.012176134775839354 88-89 0.017545610603217694 90-91 0.018403928300530958 92-93 0.018364006547167553 94-95 0.022555790650325362 96-97 0.023274382210866702 98-99 0.02892331031178889 100 0.03034053255618987 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2504900.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.4106189733132 #Duplication Level Percentage of deduplicated Percentage of total 1 82.97069832691024 59.24988924172702 2 11.324531726969473 16.173836404116273 3 2.742860280715962 5.876080512097273 4 1.0365734925233152 2.960894188496759 5 0.5498452987929924 1.9632396563186962 6 0.32839410071198233 1.4070495599416326 7 0.23257673946749194 1.162591424691802 8 0.15979512538258897 0.9128855049991096 9 0.1216961290107552 0.7821356309382786 >10 0.5082748933329271 6.0480173058618245 >50 0.017830940291244154 0.8549251796779251 >100 0.0060890706515646445 0.8035267077793534 >500 3.5737510261264733E-4 0.1620118617862946 >1k 2.9781258551022066E-4 0.5528989738403796 >5k 1.1912503420408827E-4 0.6171252566697489 >10k+ 5.956251710204414E-5 0.47289259105756903 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11118 0.44385005389436705 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9255 0.36947582737833845 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5254 0.20974889217134415 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3605 0.14391792087508481 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3372 0.13461615234141083 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2812 0.11225997045790251 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.992175336340772E-5 0.0 12-13 0.0 0.0 0.0 1.596870134536309E-4 0.0 14-15 0.0 0.0 0.0 3.193740269072618E-4 0.0 16-17 1.996087668170386E-5 0.0 0.0 3.193740269072618E-4 0.0 18-19 3.992175336340772E-5 0.0 0.0 3.9921753363407724E-4 0.0 20-21 3.992175336340772E-5 0.0 0.0 0.0011178090941754162 0.0 22-23 3.992175336340772E-5 0.0 0.0 0.0046309233901552956 0.0 24-25 3.992175336340772E-5 0.0 0.0 0.012415665296019802 3.992175336340772E-5 26-27 3.992175336340772E-5 0.0 0.0 0.026827418260209988 3.992175336340772E-5 28-29 3.992175336340772E-5 0.0 0.0 0.06740788055411394 5.988263004511158E-5 30-31 3.992175336340772E-5 0.0 0.0 0.17228232663978602 7.984350672681544E-5 32-33 3.992175336340772E-5 0.0 0.0 0.4030300610802826 7.984350672681544E-5 34-35 5.988263004511158E-5 0.0 0.0 0.747095692442812 7.984350672681544E-5 36-37 7.984350672681544E-5 0.0 0.0 1.2345403010100204 7.984350672681544E-5 38-39 7.984350672681544E-5 0.0 0.0 1.8819314144277217 1.1976526009022317E-4 40-41 7.984350672681544E-5 0.0 0.0 2.557786737993533 1.1976526009022317E-4 42-43 7.984350672681544E-5 0.0 0.0 3.199788414707174 1.1976526009022317E-4 44-45 7.984350672681544E-5 0.0 0.0 3.9683420495828177 1.1976526009022317E-4 46-47 7.984350672681544E-5 0.0 0.0 4.838396742384926 1.1976526009022317E-4 48-49 7.984350672681544E-5 0.0 0.0 5.703940277056969 1.1976526009022317E-4 50-51 7.984350672681544E-5 0.0 0.0 6.539203161802867 1.1976526009022317E-4 52-53 7.984350672681544E-5 0.0 0.0 7.338237055371472 1.1976526009022317E-4 54-55 9.980438340851931E-5 0.0 0.0 8.211784901592878 1.3972613677192702E-4 56-57 1.1976526009022317E-4 0.0 0.0 9.190945746337178 1.596870134536309E-4 58-59 1.1976526009022317E-4 0.0 0.0 10.219948900155694 1.596870134536309E-4 60-61 1.1976526009022317E-4 0.0 0.0 11.216036568326082 1.596870134536309E-4 62-63 1.1976526009022317E-4 0.0 0.0 12.207213860832766 1.596870134536309E-4 64-65 1.1976526009022317E-4 0.0 0.0 13.179607968381973 1.596870134536309E-4 66-67 1.1976526009022317E-4 0.0 0.0 14.224519940915805 1.596870134536309E-4 68-69 1.1976526009022317E-4 0.0 0.0 15.351890295021757 1.996087668170386E-4 70-71 1.1976526009022317E-4 0.0 0.0 16.479779631921435 1.996087668170386E-4 72-73 1.1976526009022317E-4 0.0 0.0 17.546708451435187 1.996087668170386E-4 74-75 1.1976526009022317E-4 0.0 0.0 18.57205876482095 1.996087668170386E-4 76-77 1.1976526009022317E-4 0.0 0.0 19.659487404686814 1.996087668170386E-4 78-79 1.1976526009022317E-4 0.0 0.0 20.784143079564053 1.996087668170386E-4 80-81 1.1976526009022317E-4 0.0 0.0 21.908060202004073 1.996087668170386E-4 82-83 1.3972613677192702E-4 0.0 0.0 23.00902231626013 1.996087668170386E-4 84-85 1.596870134536309E-4 0.0 0.0 24.059523334264842 2.3953052018044633E-4 86-87 1.596870134536309E-4 5.988263004511158E-5 0.0 25.112220048704536 2.3953052018044633E-4 88 1.596870134536309E-4 1.596870134536309E-4 0.0 25.921553754640904 2.3953052018044633E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5455 0.0 45.070194 1 TACCTGG 2070 0.0 43.64954 2 GTATCAA 9350 0.0 38.33423 1 ACCTGGG 2475 0.0 35.325077 3 ATCAACG 10965 0.0 32.365604 3 CAACGCA 11130 0.0 31.843555 5 TATCAAC 11235 0.0 31.666313 2 TCAACGC 11420 0.0 31.075459 4 AACGCAG 11900 0.0 29.824379 6 GTACATG 7120 0.0 28.577053 1 ACGCAGA 12760 0.0 27.446419 7 TACATGG 7305 0.0 26.992468 2 CGCAGAG 12980 0.0 26.870966 8 ACATGGG 7395 0.0 25.488928 3 GTACCTG 3730 0.0 24.749186 1 GTACTAG 785 0.0 24.59959 1 GCAGAGT 13745 0.0 24.484785 9 CCTGGGG 2545 0.0 24.379389 4 CATGGGG 5275 0.0 23.7917 4 CCGTATC 895 0.0 23.631039 94 >>END_MODULE