FastQCFastQC Report
Mon 6 Jun 2016
ERR1211451_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211451_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504900
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98610.3936684099165635No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89000.3553036049343287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49500.19761267914886824No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36940.14747095692442813No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27160.10842748213501538No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26150.10439538504531118No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG22200.046.3615952
GGTATCA55600.045.5770261
ACCTGGG25750.038.6939623
GTATCAA89200.033.6269761
ATCAACG105750.027.95473
CAACGCA107800.027.5489375
TATCAAC109250.027.2301162
TCAACGC110100.026.893454
CCTGGGG26750.026.8819584
AACGCAG114100.026.3145376
GTACCTG40750.025.8435651
TATCACG1659.3332346E-825.6345562
CTGGGGG16550.024.9856835
GTACATG71450.024.7410221
TACATGG72300.024.3108732
GTACTAG8150.024.2283421
ACGCAGA127600.023.1254147
ACATGGG74150.022.944683
CATGGGG53750.022.6472024
CGCAGAG130250.022.5106168
Produced by FastQC (version 0.11.5)