FastQCFastQC Report
Mon 6 Jun 2016
ERR1211450_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211450_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4125855
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185350.44924021808812964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161070.3903918096976263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83770.20303670390743253No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52530.12731906477566468No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73650.057.256841
GTATCAA136100.041.4047471
CAACGCA162200.034.024575
ATCAACG163300.033.824163
TATCAAC166500.033.3884282
AACGCAG173900.032.303856
TACCTGG25050.032.132812
GTGGTAT27050.031.8757881
TCAACGC173850.031.7711754
ACGCAGA192850.028.6420547
GTACTAG15950.028.3587881
CGCAGAG196550.028.0058468
GTACATG142800.026.3960461
ACCTGGG30050.026.1244623
TACATGG143800.025.6635932
GCAGAGT210600.025.3336099
TGGTATC33900.024.9938492
ACATGGG146150.024.6379953
CTGTGCG16100.023.065399
GAGTACT136500.022.84640312-13
Produced by FastQC (version 0.11.5)