FastQCFastQC Report
Mon 6 Jun 2016
ERR1211450_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211450_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4125855
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171170.41487158419285214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157490.3817148203220908No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86730.21021097445256803No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54940.13316027829383242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79750.057.541131
GTATCAA138450.039.6651541
TACCTGG26750.033.3777772
ATCAACG169450.031.8370743
TATCAAC171950.031.7290882
CAACGCA169300.031.7230345
GTGGTAT27450.031.5162641
AACGCAG181900.030.117926
TCAACGC179900.030.0145784
ACGCAGA201250.026.6617937
ACCTGGG33650.026.2546123
GTACATG141650.026.1890471
CGCAGAG205350.026.1294658
TACATGG143550.025.3048552
TGGTATC34200.024.5954342
TGGGGGG88200.023.9719356
GCAGAGT219350.023.8834139
ACATGGG144450.023.8787363
CATGGGG112950.023.3826354
GAGTACT142300.023.32655512-13
Produced by FastQC (version 0.11.5)