FastQCFastQC Report
Mon 6 Jun 2016
ERR1211449_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211449_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2574604
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107030.4157144166636889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87940.3415670914828067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48680.1890776212574827No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32950.12798084676323038No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32370.1257280731328002No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26290.10211279093794619No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43950.055.6156231
GTATCAA78750.041.923331
TACCTGG19850.036.979722
ATCAACG94950.034.156423
CAACGCA96200.033.6637425
TATCAAC98150.033.2232782
AACGCAG101900.032.1048166
TCAACGC101250.032.0775574
GTGGTAT16200.029.653331
GTACATG73000.029.16111
ACGCAGA112950.028.5489337
ACCTGGG25150.028.40693
CGCAGAG114550.027.8213488
TACATGG74800.027.7419222
ACATGGG75450.026.164253
GCAGAGT123750.025.1452719
CATGGGG57300.025.0186274
ATGGGGG44400.024.0304075
TGGTATC19550.023.3466472
TAACGCA2453.4015102E-1023.0214884
Produced by FastQC (version 0.11.5)