##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211449_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2574604 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.398855513313894 31.0 31.0 34.0 30.0 34.0 2 31.44224043775276 31.0 31.0 34.0 28.0 34.0 3 31.510879342997992 31.0 31.0 34.0 28.0 34.0 4 35.14182685958695 37.0 35.0 37.0 32.0 37.0 5 35.19790111411308 37.0 35.0 37.0 32.0 37.0 6 35.26715953210668 37.0 35.0 37.0 32.0 37.0 7 35.20385270899913 37.0 35.0 37.0 32.0 37.0 8 35.20551043966373 37.0 35.0 37.0 32.0 37.0 9 36.78554527220497 39.0 37.0 39.0 32.0 39.0 10-11 36.721057879192294 39.0 37.0 39.0 32.0 39.0 12-13 36.631285432633526 39.0 37.0 39.0 32.0 39.0 14-15 37.82689104809905 40.0 37.0 41.0 32.0 41.0 16-17 37.8117644499892 40.0 37.0 41.0 32.0 41.0 18-19 37.815916350631014 40.0 37.0 41.0 32.0 41.0 20-21 37.79840317190527 40.0 37.0 41.0 32.0 41.0 22-23 37.66904793902286 40.0 37.0 41.0 32.0 41.0 24-25 37.57630202547654 40.0 37.0 41.0 32.0 41.0 26-27 37.43532306327498 40.0 37.0 41.0 31.5 41.0 28-29 37.29978862768799 40.0 36.0 41.0 31.0 41.0 30-31 37.13533013232326 39.0 36.0 41.0 31.0 41.0 32-33 36.9214424820283 39.0 36.0 41.0 30.0 41.0 34-35 36.78485254431361 39.0 35.0 41.0 30.0 41.0 36-37 36.64515164273807 39.0 35.0 41.0 30.0 41.0 38-39 36.48512839256057 39.0 35.0 41.0 30.0 41.0 40-41 36.32022944111017 39.0 35.0 40.0 29.5 41.0 42-43 36.11495709631462 39.0 35.0 40.0 28.5 41.0 44-45 35.97713104617254 38.5 35.0 40.0 28.0 41.0 46-47 35.73913366871177 38.0 35.0 40.0 27.0 41.0 48-49 35.49584285583336 38.0 34.0 40.0 26.0 41.0 50-51 34.82528264540877 37.5 33.5 39.5 25.0 40.5 52-53 35.12471160613438 38.0 34.0 39.5 26.5 40.5 54-55 35.533648087239825 38.0 34.0 40.0 27.0 41.0 56-57 35.4559567218881 38.0 34.0 40.0 26.0 41.0 58-59 35.25401420956388 38.0 34.0 40.0 26.0 41.0 60-61 35.031085363030584 38.0 34.0 40.0 26.0 41.0 62-63 34.743714178957234 37.0 34.0 40.0 26.0 41.0 64-65 34.52896406593014 37.0 33.5 40.0 25.5 41.0 66-67 34.326108403467096 36.5 33.0 40.0 25.0 41.0 68-69 33.97699393770848 36.0 33.0 39.0 24.5 41.0 70-71 33.58899621067939 35.5 33.0 39.0 24.0 41.0 72-73 33.18924988075837 35.0 32.0 38.5 23.0 40.0 74-75 32.8026018758613 35.0 32.0 37.0 22.5 40.0 76-77 32.408368044173 35.0 31.5 37.0 21.5 39.0 78-79 32.006656946077925 35.0 31.0 36.0 20.0 39.0 80-81 31.596892570663293 35.0 31.0 36.0 20.0 38.0 82-83 31.225349412958266 35.0 30.5 35.5 19.0 37.0 84-85 30.888501105412715 34.0 30.0 35.0 18.0 37.0 86-87 30.543067982493618 34.0 30.0 35.0 17.5 36.0 88-89 30.21677547304362 34.0 29.0 35.0 15.5 36.0 90-91 29.896821414089313 34.0 29.0 35.0 12.5 36.0 92-93 29.59984914184861 34.0 29.0 35.0 10.5 35.0 94-95 29.30525626465274 34.0 27.5 35.0 10.0 35.0 96-97 28.992252983371422 34.0 27.0 35.0 10.0 35.0 98-99 28.657967205830488 34.0 25.5 35.0 10.0 35.0 100 27.38058474235261 32.0 24.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.25302060105048696 1101 2 -0.3525668407364968 1101 3 -0.36611631308561243 1101 4 -0.25127305302038394 1101 5 -0.2217727117491961 1101 6 -0.27790260135844136 1101 7 -0.2593668341023516 1101 8 -0.447911203250726 1101 9 -0.5753363068703052 1101 10-11 -0.4866604174014313 1101 12-13 -0.5656371614272331 1101 14-15 -0.7170441855022958 1101 16-17 -0.657960626996676 1101 18-19 -0.6977608686987296 1101 20-21 -0.6779368660352105 1101 22-23 -0.6586313707099194 1101 24-25 -0.7144105026090628 1101 26-27 -0.7432999570147061 1101 28-29 -0.9168084039629534 1101 30-31 -0.8803537898890781 1101 32-33 -0.9452902109107768 1101 34-35 -0.9134478808120079 1101 36-37 -0.9725175811260769 1101 38-39 -0.9030744335438641 1101 40-41 -1.1689188536382176 1101 42-43 -1.0657122688045177 1101 44-45 -0.9895009393670193 1101 46-47 -1.104536638728156 1101 48-49 -1.2123482903600902 1101 50-51 -1.1222090875437445 1101 52-53 -0.9237042807388676 1101 54-55 -0.958290710325322 1101 56-57 -1.0981046546263045 1101 58-59 -1.108786031949741 1101 60-61 -0.9636449425436098 1101 62-63 -1.0690289379077527 1101 64-65 -0.8739115852435191 1101 66-67 -0.8991259190600616 1101 68-69 -1.1783591911493403 1101 70-71 -0.9351288655059946 1101 72-73 -1.0669243363860161 1101 74-75 -1.1540310716571653 1101 76-77 -1.3520784702745026 1101 78-79 -1.3015492732904974 1101 80-81 -1.5152324356755251 1101 82-83 -1.3545324703908754 1101 84-85 -1.36875985080637 1101 86-87 -1.5204914899292277 1101 88-89 -1.7100430572333565 1101 90-91 -1.8266273950485221 1101 92-93 -1.96184908127481 1101 94-95 -1.9441945448409967 1101 96-97 -1.8716249594797567 1101 98-99 -2.1806484250115474 1101 100 -2.271602814858234 1102 1 -0.42611605262023744 1102 2 -0.5228063591379986 1102 3 -0.6032420211094092 1102 4 -0.6226073398732055 1102 5 -0.4166550161353868 1102 6 -0.5260987417392826 1102 7 -0.5733262881768653 1102 8 -0.5190864515927416 1102 9 -0.7505725519959725 1102 10-11 -0.6521421226358299 1102 12-13 -0.7252666408340573 1102 14-15 -0.816207365418613 1102 16-17 -0.8610727295782965 1102 18-19 -0.9457668388692326 1102 20-21 -1.1273738270120788 1102 22-23 -0.9909241936968485 1102 24-25 -0.964420998439202 1102 26-27 -0.9955394825320312 1102 28-29 -1.0027247788811167 1102 30-31 -1.0620351862970097 1102 32-33 -1.1636321188036405 1102 34-35 -1.1783250973890205 1102 36-37 -0.9865830608744588 1102 38-39 -1.1370456998746619 1102 40-41 -1.2391448519873478 1102 42-43 -1.2663884493185549 1102 44-45 -1.2016342968941274 1102 46-47 -1.4496911231160539 1102 48-49 -1.3697014198964226 1102 50-51 -1.2494213950608852 1102 52-53 -1.0028433736145175 1102 54-55 -1.0318960101858465 1102 56-57 -0.9758680821730579 1102 58-59 -0.9772305855033707 1102 60-61 -1.0370947618018533 1102 62-63 -1.0574125444884572 1102 64-65 -1.1244692874038833 1102 66-67 -1.270216489179326 1102 68-69 -1.3464940287663936 1102 70-71 -1.181411562685291 1102 72-73 -1.088546458038028 1102 74-75 -1.283937605400368 1102 76-77 -1.2177734721246125 1102 78-79 -1.356710553283662 1102 80-81 -1.503785576597167 1102 82-83 -1.5941698839336986 1102 84-85 -1.58558769072895 1102 86-87 -1.6006543709812284 1102 88-89 -1.638218312691187 1102 90-91 -1.6318185614732528 1102 92-93 -1.6125878809787935 1102 94-95 -1.608604963820305 1102 96-97 -1.867500631924468 1102 98-99 -1.8331032039942698 1102 100 -1.7435949589962263 1103 1 -0.4215029940076711 1103 2 -0.4360302260906366 1103 3 -0.5203229957586828 1103 4 -0.5307698517398762 1103 5 -0.4123689502445913 1103 6 -0.4299734296897739 1103 7 -0.4982673943264402 1103 8 -0.4690196466280767 1103 9 -0.5511966510079631 1103 10-11 -0.5543299852285699 1103 12-13 -0.7453740561851347 1103 14-15 -0.7846912443258205 1103 16-17 -0.8602965613704185 1103 18-19 -0.8169510447691621 1103 20-21 -0.7901142369835981 1103 22-23 -0.6811328713101545 1103 24-25 -0.7666739079712102 1103 26-27 -0.9056824099958973 1103 28-29 -0.9101357348953201 1103 30-31 -0.9139022092568254 1103 32-33 -0.804671463411772 1103 34-35 -0.8294317743694251 1103 36-37 -0.9632988936510927 1103 38-39 -0.8825012042521365 1103 40-41 -0.976531898856301 1103 42-43 -1.0520042856112326 1103 44-45 -1.0296044807835827 1103 46-47 -1.0853939816653266 1103 48-49 -1.0556706192916607 1103 50-51 -0.8910066065513433 1103 52-53 -0.959341035440751 1103 54-55 -0.8960358083645303 1103 56-57 -0.853731905526665 1103 58-59 -0.9727766281882282 1103 60-61 -0.8632497844803808 1103 62-63 -0.7947767370273979 1103 64-65 -0.8739040822423192 1103 66-67 -0.9234556509528247 1103 68-69 -0.8458136729420644 1103 70-71 -0.9476038555019954 1103 72-73 -1.107580598891019 1103 74-75 -1.0060693869832917 1103 76-77 -1.1003127639919903 1103 78-79 -1.0179733429183457 1103 80-81 -1.095119390457441 1103 82-83 -0.9949961558650671 1103 84-85 -1.2176444046278974 1103 86-87 -1.2378234227023341 1103 88-89 -1.172675610254565 1103 90-91 -1.2724632293822573 1103 92-93 -1.2928689892379985 1103 94-95 -1.1682916836965411 1103 96-97 -1.4480955477150523 1103 98-99 -1.4347425626666102 1103 100 -1.6226832470311052 1104 1 -0.417843477733399 1104 2 -0.3853081497990267 1104 3 -0.44197183315046473 1104 4 -0.2746827392603777 1104 5 -0.2945866305581575 1104 6 -0.30707996817000094 1104 7 -0.5135102099942799 1104 8 -0.4271158686651333 1104 9 -0.5859854086630918 1104 10-11 -0.4477936534882261 1104 12-13 -0.5697151265394709 1104 14-15 -0.8073065243195501 1104 16-17 -0.8845513188536742 1104 18-19 -0.6314491783934457 1104 20-21 -0.7489579434301561 1104 22-23 -0.7631219719748756 1104 24-25 -0.8410564533415155 1104 26-27 -0.8871030040227552 1104 28-29 -0.8434303644412253 1104 30-31 -0.9445401024754716 1104 32-33 -0.8029377330143248 1104 34-35 -0.9357229925018586 1104 36-37 -1.0755692397619683 1104 38-39 -0.9431132036094709 1104 40-41 -0.9420281418176941 1104 42-43 -0.9522471573263971 1104 44-45 -1.0906175921197132 1104 46-47 -1.1273107439238146 1104 48-49 -0.9357327197209813 1104 50-51 -0.8806648486832316 1104 52-53 -0.7734074178852381 1104 54-55 -0.7254279565088027 1104 56-57 -0.5563723881314431 1104 58-59 -0.5965876777303194 1104 60-61 -0.8543958961042222 1104 62-63 -0.8857440577040023 1104 64-65 -0.8554087472474947 1104 66-67 -0.7562101711690872 1104 68-69 -0.6767824321665117 1104 70-71 -0.5656850008102765 1104 72-73 -0.6022181782446836 1104 74-75 -0.7826251671432871 1104 76-77 -0.7889844798993408 1104 78-79 -0.7594480786552396 1104 80-81 -0.8963067529079751 1104 82-83 -1.045271721849577 1104 84-85 -0.9926393603691963 1104 86-87 -0.956665503776648 1104 88-89 -0.868172955479892 1104 90-91 -1.022060342571688 1104 92-93 -1.13020612507054 1104 94-95 -1.0922496706263054 1104 96-97 -1.2514286817690277 1104 98-99 -1.2076979564737513 1104 100 -1.1681146432445466 1105 1 -0.47059409188695867 1105 2 -0.41813360855994475 1105 3 -0.4554391640837032 1105 4 -0.3645481777272792 1105 5 -0.36681965255363735 1105 6 -0.4467862391765891 1105 7 -0.4275006726398942 1105 8 -0.29689485295261875 1105 9 -0.5218069061880044 1105 10-11 -0.4543963134215403 1105 12-13 -0.5914700020197969 1105 14-15 -0.663358629473322 1105 16-17 -0.6902617771116013 1105 18-19 -0.5846245990651866 1105 20-21 -0.7908102802070118 1105 22-23 -0.7855750700047182 1105 24-25 -0.6712408860193548 1105 26-27 -0.7354426438721546 1105 28-29 -0.6507834596861315 1105 30-31 -0.8804873008989347 1105 32-33 -0.7730469687054935 1105 34-35 -0.8717957970982511 1105 36-37 -0.8646017369017258 1105 38-39 -0.8731094543693985 1105 40-41 -0.8150157670520741 1105 42-43 -0.8357929949594833 1105 44-45 -0.7620718179527941 1105 46-47 -1.06235965928704 1105 48-49 -0.9593728245026725 1105 50-51 -0.9933638979979591 1105 52-53 -0.8082745926727597 1105 54-55 -0.790079518733215 1105 56-57 -0.717307523247122 1105 58-59 -0.7229136706490138 1105 60-61 -0.7592381372138917 1105 62-63 -0.7197624151503703 1105 64-65 -0.7958649799375692 1105 66-67 -0.7235823100527696 1105 68-69 -0.5827476455085048 1105 70-71 -0.6212334308941436 1105 72-73 -0.6006024433339476 1105 74-75 -0.6271615733252389 1105 76-77 -0.7380577128449879 1105 78-79 -0.710849193727352 1105 80-81 -0.8089028544182248 1105 82-83 -0.8631754514435421 1105 84-85 -0.8592001364221034 1105 86-87 -0.975787764809013 1105 88-89 -1.0319916332125345 1105 90-91 -1.014885255713029 1105 92-93 -0.9100940002479412 1105 94-95 -0.8564038096717681 1105 96-97 -0.8708488927684535 1105 98-99 -0.9746145787930445 1105 100 -0.9461895647691065 1106 1 -0.6597293184428459 1106 2 -0.6111894819235673 1106 3 -0.701678719295483 1106 4 -0.4181463427297416 1106 5 -0.38119244448112966 1106 6 -0.4994441480734011 1106 7 -0.5869035101373541 1106 8 -0.5368278079838689 1106 9 -0.7037113278707494 1106 10-11 -0.8202127426848378 1106 12-13 -0.7316230932633445 1106 14-15 -0.8813388694704898 1106 16-17 -0.9605558907195757 1106 18-19 -1.0180740138395876 1106 20-21 -1.0656041399351466 1106 22-23 -1.003657414823671 1106 24-25 -0.9363700116114941 1106 26-27 -0.8530798830077089 1106 28-29 -1.0116464228875515 1106 30-31 -0.9885920517069877 1106 32-33 -1.0928357035741456 1106 34-35 -1.02994225482783 1106 36-37 -0.9595668901757719 1106 38-39 -1.2110383369788167 1106 40-41 -1.0185706021567995 1106 42-43 -0.9044280345164069 1106 44-45 -0.927610397256494 1106 46-47 -0.9293258155960515 1106 48-49 -1.0023973886934314 1106 50-51 -0.983221010102632 1106 52-53 -0.8345706417244543 1106 54-55 -0.8619986773966204 1106 56-57 -0.798024461393716 1106 58-59 -0.9001439208566566 1106 60-61 -0.9228732864554701 1106 62-63 -0.9385103585630787 1106 64-65 -0.8708968642860668 1106 66-67 -0.8008028937427767 1106 68-69 -0.928531732958028 1106 70-71 -0.7668882331291442 1106 72-73 -0.8526863180309121 1106 74-75 -0.8865401018493984 1106 76-77 -0.9774822839556876 1106 78-79 -0.7508003581949474 1106 80-81 -0.9851742110461998 1106 82-83 -0.9497565949925821 1106 84-85 -0.8732936337628345 1106 86-87 -0.922000639005347 1106 88-89 -0.9810608563580274 1106 90-91 -0.8730799390213271 1106 92-93 -0.9950311224043666 1106 94-95 -0.9907545826275665 1106 96-97 -1.1105025532129424 1106 98-99 -1.044003232191848 1106 100 -1.213885043781616 1107 1 -0.6513042335294799 1107 2 -0.6607096810339854 1107 3 -0.7004545779004125 1107 4 -0.5987078895220534 1107 5 -0.5296400600917508 1107 6 -0.5724743921052848 1107 7 -0.6206282082351677 1107 8 -0.5724602387130346 1107 9 -0.7920080973363852 1107 10-11 -0.7296561660516971 1107 12-13 -0.8737917583877675 1107 14-15 -0.9040025016393187 1107 16-17 -1.0078106681274477 1107 18-19 -0.9612514640701946 1107 20-21 -0.9667204304308399 1107 22-23 -0.938778525527276 1107 24-25 -0.9899908079875175 1107 26-27 -1.0680064440255208 1107 28-29 -0.977815307756245 1107 30-31 -1.05070064303397 1107 32-33 -1.0882614986830035 1107 34-35 -1.0202805901648375 1107 36-37 -0.9334975674251851 1107 38-39 -1.047958811149627 1107 40-41 -0.8123648824831022 1107 42-43 -0.8915940711746586 1107 44-45 -0.9507973904691269 1107 46-47 -0.8248622947850492 1107 48-49 -1.0067826860746223 1107 50-51 -1.16128262038238 1107 52-53 -0.9007457112194359 1107 54-55 -0.7040776136132578 1107 56-57 -0.6505100678852926 1107 58-59 -0.8153574450795773 1107 60-61 -0.7029546263841269 1107 62-63 -0.6708634862132428 1107 64-65 -0.5001973605936882 1107 66-67 -0.5816732054116684 1107 68-69 -0.588711740134265 1107 70-71 -0.6324126903029423 1107 72-73 -0.7874758256569194 1107 74-75 -0.6370647153663995 1107 76-77 -0.5986386812346609 1107 78-79 -0.6103804047938581 1107 80-81 -0.6132631770682444 1107 82-83 -0.8086191040125463 1107 84-85 -0.7321525149293819 1107 86-87 -0.8068137881427759 1107 88-89 -0.9070265607049066 1107 90-91 -0.9156306862122854 1107 92-93 -0.7945202688170987 1107 94-95 -0.9080149935735236 1107 96-97 -1.025551614747723 1107 98-99 -0.9968565993667227 1107 100 -0.9498436530837431 1108 1 -0.48045553768074356 1108 2 -0.463077891629883 1108 3 -0.4226173574867751 1108 4 -0.3654066940286569 1108 5 -0.29731961100682014 1108 6 -0.34956616304867794 1108 7 -0.3307040204291667 1108 8 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62-63 1.4632514594436898 2311 64-65 1.4202320223475908 2311 66-67 1.5061992416063745 2311 68-69 1.5014035950115172 2311 70-71 1.4971813734958204 2311 72-73 1.5654685022894341 2311 74-75 1.5517548088352129 2311 76-77 1.6405299501914143 2311 78-79 1.580068983587779 2311 80-81 1.4750902234967285 2311 82-83 1.595473951230094 2311 84-85 1.5626005758914658 2311 86-87 1.5621807941867907 2311 88-89 1.5590482540196753 2311 90-91 1.6800790017253888 2311 92-93 1.7238157201837758 2311 94-95 1.8048797763978612 2311 96-97 1.9566197638784892 2311 98-99 1.9702099316578732 2311 100 2.0373971918571847 2312 1 0.6321565077263109 2312 2 0.6133555975651817 2312 3 0.7189952822392378 2312 4 0.5727846766771165 2312 5 0.5542357949890118 2312 6 0.3971234260570995 2312 7 0.5621337576320542 2312 8 0.5860734828408454 2312 9 0.7776883235181486 2312 10-11 0.7856718559264735 2312 12-13 0.870370155168473 2312 14-15 1.0170957752563723 2312 16-17 1.0300004141889332 2312 18-19 1.0959433608675155 2312 20-21 1.0298273956537685 2312 22-23 1.079656709401128 2312 24-25 1.0341978926784137 2312 26-27 1.071027940592991 2312 28-29 1.1693234326827664 2312 30-31 1.0622704965815615 2312 32-33 1.1489575356044455 2312 34-35 1.217176515774618 2312 36-37 1.2955261471566004 2312 38-39 1.3185782072377634 2312 40-41 1.303413113811935 2312 42-43 1.2639412161996901 2312 44-45 1.2769176048328745 2312 46-47 1.2532138028480873 2312 48-49 1.154211986464837 2312 50-51 1.0866097571949425 2312 52-53 0.9994863411606403 2312 54-55 1.0475682388789593 2312 56-57 1.123674786384889 2312 58-59 1.1663573969064416 2312 60-61 1.0503578626334047 2312 62-63 1.1140095570847492 2312 64-65 1.1712931137060423 2312 66-67 1.1463547650382964 2312 68-69 1.1396192183308713 2312 70-71 1.0486785227207136 2312 72-73 1.1559348530713223 2312 74-75 1.1780961301064679 2312 76-77 1.1611757686349264 2312 78-79 1.255618860477071 2312 80-81 1.1222821352280477 2312 82-83 1.235870670917972 2312 84-85 1.3647408254615243 2312 86-87 1.3761554782105492 2312 88-89 1.4825988867253912 2312 90-91 1.4842546507063368 2312 92-93 1.5807776244107323 2312 94-95 1.6548788402357246 2312 96-97 1.7793607454627818 2312 98-99 1.646534402010694 2312 100 1.8464548725725258 2313 1 0.7000645392556315 2313 2 0.7045521005900355 2313 3 0.7527851537511232 2313 4 0.6131043385612429 2313 5 0.6272586976895838 2313 6 0.6954910529782907 2313 7 0.6911074962547943 2313 8 0.7129957376506368 2313 9 0.9177546795242506 2313 10-11 0.7331525077686294 2313 12-13 0.9609492543890994 2313 14-15 1.1813933144977042 2313 16-17 1.0857747577822323 2313 18-19 1.1175259882315345 2313 20-21 1.19970051704302 2313 22-23 1.2804663386863382 2313 24-25 1.2470318868467203 2313 26-27 1.3242389726196464 2313 28-29 1.305874419166301 2313 30-31 1.343318882304807 2313 32-33 1.2794154916827623 2313 34-35 1.2559624614498972 2313 36-37 1.325789046212357 2313 38-39 1.3755049669663464 2313 40-41 1.369356417407694 2313 42-43 1.3309813695883435 2313 44-45 1.36441660421292 2313 46-47 1.4630188991119795 2313 48-49 1.4039888695038627 2313 50-51 1.4098381063338152 2313 52-53 1.2855923285298374 2313 54-55 1.2879658000913494 2313 56-57 1.3552661468192753 2313 58-59 1.3241533547424424 2313 60-61 1.2971105315210139 2313 62-63 1.2428499151959969 2313 64-65 1.2448204778942369 2313 66-67 1.2504110441627247 2313 68-69 1.2577922225411342 2313 70-71 1.2013905860246084 2313 72-73 1.2597756795578547 2313 74-75 1.3034784946693634 2313 76-77 1.2062329402272027 2313 78-79 1.3057995256040655 2313 80-81 1.4032910283976037 2313 82-83 1.4740974190649005 2313 84-85 1.435479227637508 2313 86-87 1.5578195219755386 2313 88-89 1.54038543447583 2313 90-91 1.5593207342031796 2313 92-93 1.6302533847115832 2313 94-95 1.7516195229736269 2313 96-97 1.8639591179011923 2313 98-99 1.8809248371058018 2313 100 1.9958889474716983 2314 1 0.38262986835032464 2314 2 0.3765533102029579 2314 3 0.4439877189302628 2314 4 0.4015164216946445 2314 5 0.4038883012164547 2314 6 0.5075293257310065 2314 7 0.5021323433037352 2314 8 0.5326403263822428 2314 9 0.6239387006374955 2314 10-11 0.6416018039938436 2314 12-13 0.4303877840595973 2314 14-15 0.6959436583876553 2314 16-17 0.7242248287595245 2314 18-19 0.7621650162425695 2314 20-21 0.7779723450580533 2314 22-23 0.6990545779497737 2314 24-25 0.8080798514980287 2314 26-27 0.8090722634665752 2314 28-29 0.8665849917830641 2314 30-31 0.8289253632309084 2314 32-33 0.7989014436781261 2314 34-35 0.8425816183196986 2314 36-37 0.8751760548695913 2314 38-39 0.9343074001877198 2314 40-41 0.9134188799196963 2314 42-43 1.0061818679288734 2314 44-45 0.9833244911890802 2314 46-47 0.88623284564909 2314 48-49 1.0304434040528037 2314 50-51 1.1124782614841635 2314 52-53 0.9136257328404582 2314 54-55 0.9407016121314982 2314 56-57 0.9780187409891354 2314 58-59 0.9858881621040823 2314 60-61 0.9630405589122546 2314 62-63 0.8895838978215522 2314 64-65 0.8590861003950323 2314 66-67 0.9254640896881554 2314 68-69 0.8762261986687747 2314 70-71 0.8889568148313955 2314 72-73 0.8963135499393431 2314 74-75 0.9429913505554879 2314 76-77 0.9513060793226842 2314 78-79 0.9961942243004813 2314 80-81 0.934333907176395 2314 82-83 0.9319134179452639 2314 84-85 0.90769780519269 2314 86-87 1.1518819220075258 2314 88-89 1.2352352700284506 2314 90-91 1.3020730534610152 2314 92-93 1.4131578552810034 2314 94-95 1.4303276488733374 2314 96-97 1.5460470997470033 2314 98-99 1.6435005003935466 2314 100 1.51550204628008 2315 1 0.4545923497902251 2315 2 0.4455431579799729 2315 3 0.5790110774932593 2315 4 0.497743440369419 2315 5 0.35665752827134156 2315 6 -0.3105267831066172 2315 7 0.29882049743403627 2315 8 0.3826240297103851 2315 9 0.6215479185179618 2315 10-11 0.7092924448755511 2315 12-13 0.6898579611501034 2315 14-15 0.8485218522335529 2315 16-17 0.7711803690243784 2315 18-19 0.880036429465811 2315 20-21 0.8925795952662909 2315 22-23 0.7575146631753356 2315 24-25 0.8667400236391671 2315 26-27 0.9824709645614718 2315 28-29 1.0425292933801344 2315 30-31 1.0541716240500847 2315 32-33 1.1021595708887375 2315 34-35 1.1015734579121599 2315 36-37 1.119269416691914 2315 38-39 1.1841945306455983 2315 40-41 1.2952930575413504 2315 42-43 1.369614550602492 2315 44-45 1.1901751291736105 2315 46-47 1.2595388971465695 2315 48-49 1.3321630047239879 2315 50-51 1.2393683736294108 2315 52-53 1.1090089460612873 2315 54-55 0.9531872770960632 2315 56-57 0.9445583539733562 2315 58-59 0.9962181395301783 2315 60-61 0.8550962331719631 2315 62-63 0.9928124495906516 2315 64-65 1.0976156577291363 2315 66-67 1.0906411198309627 2315 68-69 1.1442542410159788 2315 70-71 1.1052157629114063 2315 72-73 1.2167362976789278 2315 74-75 1.21392955599012 2315 76-77 1.2536220174677624 2315 78-79 1.2732044489531233 2315 80-81 1.2652658957325116 2315 82-83 1.4457218294422596 2315 84-85 1.4053882046422288 2315 86-87 1.4295422027931437 2315 88-89 1.636360645757275 2315 90-91 1.7813922750836788 2315 92-93 1.7864542494914595 2315 94-95 1.910041913893437 2315 96-97 1.9416050495049682 2315 98-99 2.084265417884101 2315 100 2.1419962096572327 2316 1 0.04299727418471022 2316 2 0.04969299936976057 2316 3 0.1784254397857481 2316 4 0.10154733045214925 2316 5 0.04630260348328363 2316 6 0.1565275536822952 2316 7 0.21684971634761752 2316 8 0.25925677032168437 2316 9 0.4700531254983886 2316 10-11 0.23465177768937195 2316 12-13 0.3769127308701101 2316 14-15 0.6210346630584027 2316 16-17 0.5840803383256201 2316 18-19 0.42733198759271573 2316 20-21 0.49007528563396363 2316 22-23 0.37946072783162776 2316 24-25 0.4815649620448781 2316 26-27 0.5115623602526185 2316 28-29 0.6795120391078981 2316 30-31 0.7453012593646733 2316 32-33 0.8022925988088545 2316 34-35 0.7690439989186046 2316 36-37 0.7017601086732483 2316 38-39 0.8350564571102765 2316 40-41 0.874366545627872 2316 42-43 0.9463508011043018 2316 44-45 0.7594622097224075 2316 46-47 0.8451799500350106 2316 48-49 0.876575044931478 2316 50-51 0.7313695779650189 2316 52-53 0.3792384389021777 2316 54-55 0.3887576835002946 2316 56-57 0.4290322639709174 2316 58-59 0.5060317036551467 2316 60-61 0.6977818622208503 2316 62-63 0.6726557678894594 2316 64-65 0.7474574410904182 2316 66-67 0.6844649641017142 2316 68-69 0.5696163288458891 2316 70-71 0.4813653917713836 2316 72-73 0.6552836957630106 2316 74-75 0.7749157730381597 2316 76-77 0.8406268033242981 2316 78-79 0.961130000546703 2316 80-81 1.0080050508084284 2316 82-83 0.9990097443673669 2316 84-85 1.0804246102120842 2316 86-87 1.1257739311328194 2316 88-89 1.2076748277929958 2316 90-91 1.284791374586991 2316 92-93 1.4704735818329837 2316 94-95 1.596521475609734 2316 96-97 1.5714735565223883 2316 98-99 1.7462694165931794 2316 100 1.6323587697756174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 12.0 1 140.0 2 72.0 3 47.0 4 50.0 5 34.0 6 56.0 7 330.0 8 1151.0 9 1951.0 10 2419.0 11 3030.0 12 3278.0 13 3979.0 14 4707.0 15 5817.0 16 7893.0 17 10794.0 18 14132.0 19 17088.0 20 20997.0 21 24853.0 22 26522.0 23 27801.0 24 27998.0 25 29607.0 26 32414.0 27 37695.0 28 45346.0 29 53169.0 30 64628.0 31 79148.0 32 98700.0 33 126287.0 34 172443.0 35 235867.0 36 334529.0 37 482603.0 38 458820.0 39 118069.0 40 128.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.351327049545645 18.87810450965242 12.223919783421467 23.546648657380466 2 16.54136157262042 21.01235728133587 39.510469925221486 22.935811220822224 3 19.223528113704248 26.438591502018667 30.40071080693837 23.937169577338715 4 12.938624265446885 17.15923068017501 38.386504502306515 31.515640552071588 5 12.973591028082698 37.410301418315726 34.83059546260186 14.785512090999722 6 29.405683830653295 36.9979468816346 19.11463691128298 14.481732376429129 7 25.984959191605128 33.25670423630004 23.05211815001943 17.7062184220754 8 25.37231125807997 34.824456713727706 21.27621704324955 18.527014984942774 9 26.68492969704741 16.03948700313386 21.044062475106905 36.23152082471182 10-11 24.428737881281 25.93380122726468 29.252521861247168 20.384939030207157 12-13 25.10886920191599 24.28823614212701 28.55664326534165 22.04625139061535 14-15 22.78480078319244 25.757532788413723 26.688001149925412 24.769665278468423 16-17 21.82459743014939 28.14462504348576 27.502929643681906 22.52784788268295 18-19 21.350788680053036 27.544577525988583 29.632987854836095 21.471645939122283 20-21 22.3218215758527 27.073349818475663 29.351310296868533 21.253518308803105 22-23 22.272931328443686 26.82847563881139 29.06924227657943 21.829350756165496 24-25 21.837283780494698 26.674594561414995 29.46744865597126 22.020673002119047 26-27 21.810772009627417 26.876218990939186 29.261881744209106 22.05112725522429 28-29 21.5396529902032 27.582333439260076 29.219969962386944 21.65804360814978 30-31 22.491941538153746 26.934424175676895 29.251162759077488 21.322471527091874 32-33 22.23206017720363 27.051399775013437 28.933229186557607 21.783310861225328 34-35 22.289374638803768 27.089401834755456 29.169600753716374 21.451622772724402 36-37 22.08809823053717 26.81960958761745 29.173486612961813 21.918805568883563 38-39 21.9178470695188 26.96221415861817 29.201372971040957 21.918565800822073 40-41 21.89098341580687 26.43022008526172 29.67716632406835 22.00163017486306 42-43 21.577394163600022 26.712320384428367 29.22137753368517 22.488907918286436 44-45 21.208482211637193 27.040502993821182 29.542474491951875 22.20854030258975 46-47 21.128768081447877 26.535181713086647 29.293393046232165 23.04265715923332 48-49 21.311121239780178 26.606621274168436 29.346937871495165 22.735319614556218 50-51 21.201882534494256 26.87242811944113 29.15861540042516 22.767073945639456 52-53 20.976753611440813 27.179624578411847 28.889952742491754 22.953669067655586 54-55 20.768450927957314 26.87584855189543 29.22764388758975 23.128056632557502 56-57 21.19751336828783 26.693423319205685 28.842705156247995 23.266358156258487 58-59 20.929079857973964 26.749629177124902 28.992791832842336 23.3284991320588 60-61 21.308859669212755 26.66436281505802 28.752650837690936 23.274126678038293 62-63 21.336308043762045 26.43262028345869 28.750893578666002 23.48017809411326 64-65 21.02648858677937 26.199745056239536 28.71000088970011 24.063765467280987 66-67 21.090973164427908 26.22714034163407 28.39802109608244 24.28386539785558 68-69 20.737105157276545 26.274369466849524 28.615959796948715 24.37256557892522 70-71 20.75094859425423 25.99534882817281 28.761688643485932 24.49201393408703 72-73 20.776441394146214 26.031962590238926 28.614589153998182 24.57700686161668 74-75 20.92242661235257 25.94158515181625 28.556801062971132 24.57918717286005 76-77 20.75362299697915 25.753133507788316 28.64380026976293 24.8494432254696 78-79 20.68485208123513 25.77268264162781 28.889499666392464 24.65296561074459 80-81 20.805708865645194 25.707727352986936 28.769228348604127 24.71733543276374 82-83 21.08276291951129 25.378916712621567 28.960368970446353 24.57795139742079 84-85 21.187815584772256 25.44959510171451 28.664250335578163 24.69833897793507 86-87 21.024534505557344 25.459822892242094 28.931200208837364 24.584442393363197 88-89 21.26326119095241 24.892200165533616 29.02249893603569 24.822039707478286 90-91 21.804148933008584 24.691215339362536 28.79510318915451 24.709532538474374 92-93 21.904940979757967 24.552960685481327 28.727820426331874 24.814277908428835 94-95 21.62025975438719 24.267979036274703 28.94569478276748 25.166066426570627 96-97 21.826788668328962 24.05699557956358 28.822679037776123 25.29353671433134 98-99 21.916700905957054 24.054767323381114 28.643026500725092 25.385505269936736 100 22.188273059539206 23.893817000112286 28.701137586191223 25.216772354157285 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1865.0 1 1278.0 2 1575.5 3 2410.5 4 1677.0 5 1053.5 6 1407.5 7 2142.5 8 3188.0 9 3736.5 10 3027.5 11 2300.5 12 2290.5 13 2447.0 14 2662.5 15 2975.5 16 3237.5 17 3481.5 18 3824.5 19 4140.5 20 4528.0 21 5040.0 22 5770.0 23 6783.0 24 8007.0 25 9427.5 26 11265.5 27 13534.0 28 16129.0 29 19742.5 30 24513.0 31 29257.0 32 34512.5 33 40290.5 34 46090.5 35 51623.0 36 57410.5 37 64938.0 38 70349.5 39 73145.5 40 75684.0 41 78612.0 42 81968.0 43 86038.0 44 91089.0 45 96797.5 46 102917.5 47 108541.5 48 112696.5 49 115364.0 50 115420.0 51 113224.5 52 108444.5 53 101622.0 54 94073.0 55 83654.0 56 73376.0 57 62725.0 58 51726.0 59 42763.5 60 33799.0 61 25627.5 62 19753.5 63 15210.5 64 11110.5 65 7818.0 66 5619.5 67 4113.0 68 3101.0 69 2323.5 70 1774.5 71 1352.5 72 1033.0 73 787.0 74 601.5 75 463.0 76 334.0 77 228.0 78 150.5 79 106.0 80 85.0 81 64.5 82 54.0 83 44.5 84 28.0 85 22.5 86 23.5 87 19.5 88 14.5 89 14.0 90 11.0 91 9.0 92 9.0 93 6.0 94 3.0 95 2.0 96 2.0 97 1.0 98 1.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21591670019933162 2 0.14806160481378883 3 0.024392100688105822 4 0.022916145550927445 5 0.027421692811787756 6 0.05612513613744094 7 0.06230084315879258 8 0.05519295394553881 9 0.04268617620418519 10-11 0.04495837029694664 12-13 0.04284153990283554 14-15 0.020352644523196576 16-17 0.018857268923686905 18-19 0.01914857585865632 20-21 0.022100486133013075 22-23 0.025770953513627726 24-25 0.02215874752000696 26-27 0.019323360019637973 28-29 0.019342780481969267 30-31 0.01734247286184594 32-33 0.026120521835591028 34-35 0.026858499404180215 36-37 0.025596169352646077 38-39 0.024236736989455467 40-41 0.02513007825669501 42-43 0.03140288758970312 44-45 0.040918914132037396 46-47 0.045793450177192295 48-49 0.04082181182038092 50-51 0.02848981824000895 52-53 0.04468648382430851 54-55 0.04280269897817295 56-57 0.045171995382590875 58-59 0.04944449709547565 60-61 0.025382544267001837 62-63 0.0265671924692108 64-65 0.027305170037799985 66-67 0.033247831511176086 68-69 0.037617435535717336 70-71 0.04113253921768163 72-73 0.04497779075927793 74-75 0.02666429478086727 76-77 0.019109734933993733 78-79 0.01831349597841066 80-81 0.02223642936933214 82-83 0.020294383136202694 84-85 0.012254311731046795 86-87 0.01367200548123129 88-89 0.01959524649227609 90-91 0.02021670128687752 92-93 0.020566269608840816 94-95 0.024625146236081356 96-97 0.02524660103068278 98-99 0.03132520574037794 100 0.031888399147985476 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2574604.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.65429585624857 #Duplication Level Percentage of deduplicated Percentage of total 1 83.55396373481507 60.705544011515165 2 11.014401496819522 16.00487170058865 3 2.644722007340272 5.764512455364952 4 1.0125887971875032 2.942757042063349 5 0.5374663131458545 1.9524618264033025 6 0.309153753199026 1.3476808949995012 7 0.19749835915706293 1.004437295212462 8 0.14830627320451747 0.8620070280590912 9 0.10885670928238672 0.7118016805926147 >10 0.4509781047799548 5.344957123904171 >50 0.014522736579009323 0.728404463498836 >100 0.006579948271849538 0.8702534577779266 >500 4.5259586729273814E-4 0.20116196077354523 >1k 3.960213838745917E-4 0.7577484157758005 >5k 5.657448341065596E-5 0.3614667517402978 >10k+ 5.657448341065596E-5 0.43993389173031694 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10703 0.4157144166636889 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8794 0.3415670914828067 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4868 0.1890776212574827 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3295 0.12798084676323038 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3237 0.1257280731328002 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2629 0.10211279093794619 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.884092466258889E-5 2 0.0 0.0 0.0 0.0 3.884092466258889E-5 3 0.0 0.0 0.0 0.0 3.884092466258889E-5 4 0.0 0.0 0.0 0.0 7.768184932517778E-5 5 0.0 0.0 0.0 0.0 7.768184932517778E-5 6 0.0 0.0 0.0 0.0 7.768184932517778E-5 7 0.0 3.884092466258889E-5 0.0 0.0 7.768184932517778E-5 8 0.0 3.884092466258889E-5 0.0 0.0 7.768184932517778E-5 9 0.0 3.884092466258889E-5 0.0 7.768184932517778E-5 7.768184932517778E-5 10-11 0.0 3.884092466258889E-5 0.0 7.768184932517778E-5 7.768184932517778E-5 12-13 0.0 3.884092466258889E-5 0.0 1.7478416098165E-4 1.1652277398776667E-4 14-15 0.0 3.884092466258889E-5 0.0 2.524660103068278E-4 1.1652277398776667E-4 16-17 0.0 7.768184932517778E-5 0.0 3.107273973007111E-4 1.1652277398776667E-4 18-19 0.0 7.768184932517778E-5 0.0 5.243524829449499E-4 2.3304554797553333E-4 20-21 0.0 7.768184932517778E-5 0.0 0.0013011709761967278 2.3304554797553333E-4 22-23 0.0 7.768184932517778E-5 0.0 0.005224104367118205 2.3304554797553333E-4 24-25 0.0 7.768184932517778E-5 0.0 0.01547810847804167 2.3304554797553333E-4 26-27 0.0 1.1652277398776667E-4 0.0 0.030490125860132278 2.3304554797553333E-4 28-29 0.0 1.359432363190611E-4 0.0 0.07234122218407181 3.107273973007111E-4 30-31 0.0 1.5536369865035556E-4 0.0 0.1937385322169934 3.301478596320056E-4 32-33 0.0 1.5536369865035556E-4 0.0 0.451273283192289 4.2725017128847776E-4 34-35 0.0 1.5536369865035556E-4 0.0 0.8177762483084777 4.6609109595106667E-4 36-37 0.0 1.9420462331294446E-4 0.0 1.3416432196951453 4.855115582823611E-4 38-39 0.0 2.3304554797553333E-4 0.0 2.048004275608987 5.049320206136556E-4 40-41 0.0 2.3304554797553333E-4 0.0 2.8066063751940105 5.437729452762444E-4 42-43 0.0 2.718864726381222E-4 0.0 3.52794060756528 6.020343322701277E-4 44-45 0.0 2.718864726381222E-4 0.0 4.359214077194007 6.214547946014222E-4 46-47 0.0 2.9130693496941663E-4 0.0 5.300601568241174 6.214547946014222E-4 48-49 0.0 3.107273973007111E-4 0.0 6.227481973926864 6.602957192640111E-4 50-51 0.0 3.107273973007111E-4 0.0 7.144652148446907 6.602957192640111E-4 52-53 0.0 3.107273973007111E-4 0.0 8.007445028439324 6.602957192640111E-4 54-55 0.0 3.107273973007111E-4 0.0 8.938675617687224 6.602957192640111E-4 56-57 0.0 3.107273973007111E-4 0.0 9.976077874500312 6.602957192640111E-4 58-59 0.0 3.107273973007111E-4 0.0 11.064847254179671 6.797161815953055E-4 60-61 0.0 3.107273973007111E-4 0.0 12.11667891450491 6.991366439266E-4 62-63 0.0 3.107273973007111E-4 0.0 13.153925807619347 6.991366439266E-4 64-65 0.0 3.107273973007111E-4 0.0 14.184123072907521 6.991366439266E-4 66-67 0.0 3.495683219633E-4 0.0 15.286836344540752 7.185571062578945E-4 68-69 0.0 3.495683219633E-4 0.0 16.460725610618177 7.379775685891889E-4 70-71 0.0 3.495683219633E-4 0.0 17.649743416851678 7.379775685891889E-4 72-73 0.0 3.495683219633E-4 0.0 18.760652123588713 7.379775685891889E-4 74-75 0.0 3.495683219633E-4 0.0 19.824466209172364 7.379775685891889E-4 76-77 0.0 3.495683219633E-4 0.0 20.93826856479676 7.573980309204832E-4 78-79 0.0 3.495683219633E-4 0.0 22.11075955758633 7.768184932517777E-4 80-81 0.0 3.495683219633E-4 0.0 23.252702163128774 7.962389555830722E-4 82-83 0.0 3.8840924662588886E-4 0.0 24.37021382705845 8.156594179143667E-4 84-85 0.0 4.078297089571833E-4 0.0 25.456167239699774 8.156594179143667E-4 86-87 0.0 4.2725017128847776E-4 0.0 26.540761219977906 8.545003425769555E-4 88 0.0 4.2725017128847776E-4 0.0 27.366383335068228 8.933412672395444E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4395 0.0 55.615623 1 GTATCAA 7875 0.0 41.92333 1 TACCTGG 1985 0.0 36.97972 2 ATCAACG 9495 0.0 34.15642 3 CAACGCA 9620 0.0 33.663742 5 TATCAAC 9815 0.0 33.223278 2 AACGCAG 10190 0.0 32.104816 6 TCAACGC 10125 0.0 32.077557 4 GTGGTAT 1620 0.0 29.65333 1 GTACATG 7300 0.0 29.1611 1 ACGCAGA 11295 0.0 28.548933 7 ACCTGGG 2515 0.0 28.4069 3 CGCAGAG 11455 0.0 27.821348 8 TACATGG 7480 0.0 27.741922 2 ACATGGG 7545 0.0 26.16425 3 GCAGAGT 12375 0.0 25.145271 9 CATGGGG 5730 0.0 25.018627 4 ATGGGGG 4440 0.0 24.030407 5 TGGTATC 1955 0.0 23.346647 2 TAACGCA 245 3.4015102E-10 23.021488 4 >>END_MODULE