FastQCFastQC Report
Mon 6 Jun 2016
ERR1211449_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211449_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2574604
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99330.3858069046734954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86940.3376829990165478No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48350.18779587074361725No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33370.1296121655990591No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26190.10172438169132031No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44150.053.9896771
TACCTGG21450.042.7231832
GTATCAA75650.039.090861
ACCTGGG25750.034.6762923
ATCAACG92200.031.6021823
CAACGCA93550.031.2405415
TATCAAC95200.030.853142
TCAACGC96950.030.102334
GTGGTAT17500.030.0893961
AACGCAG101700.029.4773566
CCTGGGG24950.027.1236574
CTGGGGG16550.026.6871265
GTACATG71600.026.2651841
ACGCAGA112350.026.013947
TGGGGGG45050.025.9712816
TGGTATC19900.025.7412552
CGCAGAG114050.025.3789848
TACATGG73300.025.2608852
ACATGGG73800.023.6251133
CATGGGG55950.023.4347554
Produced by FastQC (version 0.11.5)