##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211449_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2574604 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.032851265670374 33.0 31.0 34.0 30.0 34.0 2 32.162050940649515 33.0 31.0 34.0 30.0 34.0 3 32.24117689555364 34.0 31.0 34.0 30.0 34.0 4 35.89092458490704 37.0 35.0 37.0 35.0 37.0 5 35.75188533848312 37.0 35.0 37.0 35.0 37.0 6 35.787573933700095 37.0 35.0 37.0 33.0 37.0 7 35.71993557067417 37.0 35.0 37.0 33.0 37.0 8 35.7075453157068 37.0 35.0 37.0 33.0 37.0 9 37.29047418554465 39.0 37.0 39.0 34.0 39.0 10-11 37.26988674763187 39.0 37.0 39.0 34.0 39.0 12-13 37.189920857731906 39.0 37.0 39.0 33.0 39.0 14-15 38.38403575850888 40.0 38.0 41.0 33.5 41.0 16-17 38.36955372554381 40.0 38.0 41.0 33.0 41.0 18-19 38.380867504284154 40.0 38.0 41.0 33.5 41.0 20-21 38.33781816543437 40.0 38.0 41.0 34.0 41.0 22-23 38.22969241094941 40.0 38.0 41.0 33.5 41.0 24-25 38.111221958794445 40.0 37.0 41.0 33.0 41.0 26-27 37.98260237302513 40.0 37.0 41.0 33.0 41.0 28-29 37.89032216216552 40.0 37.0 41.0 33.0 41.0 30-31 37.930343462528604 40.0 37.0 41.0 33.0 41.0 32-33 37.75245688268953 40.0 37.0 41.0 33.0 41.0 34-35 37.630041552021204 40.0 37.0 41.0 33.0 41.0 36-37 37.47625362968441 40.0 36.0 41.0 32.0 41.0 38-39 37.35217746107751 40.0 36.0 41.0 32.0 41.0 40-41 37.28246343903761 40.0 36.0 41.0 31.5 41.0 42-43 37.13755358105557 39.0 36.0 41.0 31.0 41.0 44-45 36.97667718996786 39.0 36.0 41.0 31.0 41.0 46-47 36.75491337696982 39.0 35.0 41.0 30.0 41.0 48-49 36.53633316036175 39.0 35.0 41.0 30.0 41.0 50-51 36.35397793214024 39.0 35.0 41.0 29.5 41.0 52-53 36.1501351275769 39.0 35.0 40.0 29.0 41.0 54-55 35.95361247788009 38.5 35.0 40.0 28.0 41.0 56-57 35.726046413351334 38.0 35.0 40.0 28.0 41.0 58-59 35.465002190628155 38.0 34.0 40.0 27.5 41.0 60-61 35.20904515801265 38.0 34.0 40.0 27.0 41.0 62-63 34.91335987981064 37.0 34.0 40.0 26.0 41.0 64-65 34.56141313382563 36.5 33.5 39.5 26.0 41.0 66-67 34.19937046629307 36.0 33.0 39.0 26.0 41.0 68-69 33.818565884306864 35.5 33.0 39.0 25.5 40.0 70-71 33.422853961230544 35.0 32.5 38.0 25.0 40.0 72-73 33.051513941561495 35.0 32.0 37.5 24.0 40.0 74-75 32.66282426345954 35.0 32.0 37.0 24.0 39.0 76-77 31.61598133149797 34.0 30.5 36.0 22.5 38.5 78-79 32.08282535877362 34.5 31.0 36.0 23.5 38.0 80-81 31.95535915426217 35.0 31.0 35.5 23.0 37.0 82-83 31.67052078688606 34.0 31.0 35.0 23.0 37.0 84-85 31.39011067333073 34.0 31.0 35.0 22.0 36.5 86-87 31.13134078095117 34.0 31.0 35.0 20.5 36.0 88-89 30.894578739099295 34.0 30.0 35.0 20.0 36.0 90-91 30.67105135391695 34.0 30.0 35.0 20.0 35.0 92-93 30.486518315049615 34.0 30.0 35.0 19.0 35.0 94-95 30.313243706604975 34.0 29.5 35.0 19.0 35.0 96-97 30.139210729106303 34.0 29.0 35.0 18.5 35.0 98-99 29.93465577618927 34.0 29.0 35.0 18.0 35.0 100 28.06845984858254 32.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3247208795817791 1101 2 -0.26329277951017716 1101 3 -0.30643931541506575 1101 4 -0.1497934569909134 1101 5 -0.2139160148089232 1101 6 -0.22718654600168264 1101 7 -0.1974163515147609 1101 8 -0.31850787548449944 1101 9 -0.4579406165566482 1101 10-11 -0.5329876301441132 1101 12-13 -0.541975894206594 1101 14-15 -0.7653125228808406 1101 16-17 -0.7749273948628925 1101 18-19 -0.8402565178415387 1101 20-21 -0.7128554571757064 1101 22-23 -0.7712136782986079 1101 24-25 -0.7088049836457131 1101 26-27 -0.8168269400935628 1101 28-29 -0.7440762933370095 1101 30-31 -0.6599537813403771 1101 32-33 -0.5300455068159309 1101 34-35 -0.7091789014527166 1101 36-37 -0.7404890799288353 1101 38-39 -0.7064381645774276 1101 40-41 -0.7185658175781882 1101 42-43 -0.684137165907984 1101 44-45 -0.8177056843754187 1101 46-47 -0.6474162646436739 1101 48-49 -0.6312489239052397 1101 50-51 -0.7788212335405902 1101 52-53 -0.7740747288956129 1101 54-55 -0.6276536487951674 1101 56-57 -0.727448178004316 1101 58-59 -0.7319667259625859 1101 60-61 -0.7318462630594311 1101 62-63 -0.7943100558751794 1101 64-65 -0.888678956703906 1101 66-67 -0.8712396319955289 1101 68-69 -0.8948259887733272 1101 70-71 -0.9438525565985287 1101 72-73 -0.9474772165662486 1101 74-75 -0.9961056396077694 1101 76-77 -0.8496228146562039 1101 78-79 -0.9334596134763053 1101 80-81 -0.7664321979719446 1101 82-83 -0.8985938052115294 1101 84-85 -0.8079290674120969 1101 86-87 -1.065571116081479 1101 88-89 -1.0805437993734692 1101 90-91 -1.09970968090105 1101 92-93 -1.1506056956946402 1101 94-95 -1.1496249225882522 1101 96-97 -1.3180593986692486 1101 98-99 -1.5419334417122421 1101 100 -1.3941734976989473 1102 1 -0.33608306043738523 1102 2 -0.2868817129915229 1102 3 -0.30586257844412756 1102 4 -0.20955742533976007 1102 5 -0.20049985336526532 1102 6 -0.38097963227384213 1102 7 -0.3694227272868247 1102 8 -0.34560561834733505 1102 9 -0.49990365700295314 1102 10-11 -0.5791458005252323 1102 12-13 -0.48504046313039595 1102 14-15 -0.7686740348496457 1102 16-17 -0.7508286130907678 1102 18-19 -0.8965819455021418 1102 20-21 -0.8009892985783722 1102 22-23 -0.7459973574606522 1102 24-25 -0.8667494375779157 1102 26-27 -0.8965324520054381 1102 28-29 -0.7695060886861143 1102 30-31 -0.7492994463846401 1102 32-33 -0.6826372652766466 1102 34-35 -0.751139629258482 1102 36-37 -0.6990261035248935 1102 38-39 -0.8767659191101984 1102 40-41 -0.8492469080059877 1102 42-43 -0.9815514558917613 1102 44-45 -0.8230044775328551 1102 46-47 -0.8519139946980303 1102 48-49 -0.9112643296491214 1102 50-51 -0.9227430805220607 1102 52-53 -0.8973576822443121 1102 54-55 -0.9186939456670515 1102 56-57 -0.9707297793477849 1102 58-59 -1.0766248821120783 1102 60-61 -1.0538798568316636 1102 62-63 -1.0839778895358378 1102 64-65 -0.8755812280727113 1102 66-67 -0.9885819454899618 1102 68-69 -1.0549567774904958 1102 70-71 -1.0422760473337362 1102 72-73 -1.1373163635221033 1102 74-75 -1.0170572733993595 1102 76-77 -0.9925911421545663 1102 78-79 -1.0201793641380767 1102 80-81 -1.1099793971863576 1102 82-83 -1.0140623461844065 1102 84-85 -1.1440194240568466 1102 86-87 -1.1031187920556356 1102 88-89 -1.0911002918449348 1102 90-91 -1.1428551922436618 1102 92-93 -1.1441895413667993 1102 94-95 -1.3318567274440838 1102 96-97 -1.3475758077433397 1102 98-99 -1.2328903056293576 1102 100 -1.307624170855238 1103 1 -0.40855941499594195 1103 2 -0.3981067050804086 1103 3 -0.35340310092927396 1103 4 -0.21314379712696763 1103 5 -0.2046523093267325 1103 6 -0.3294624563658246 1103 7 -0.30469926468002484 1103 8 -0.4552325653604541 1103 9 -0.5804546221588893 1103 10-11 -0.5214430122969773 1103 12-13 -0.5567468025699398 1103 14-15 -0.7105906341253387 1103 16-17 -0.7666490835383613 1103 18-19 -0.8018586586978742 1103 20-21 -0.7339513955510597 1103 22-23 -0.8229443705755273 1103 24-25 -0.639882414618107 1103 26-27 -0.8409190769483033 1103 28-29 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-0.8668997133016809 1103 94-95 -0.7150711010596424 1103 96-97 -0.8521730441274329 1103 98-99 -1.1601757386310076 1103 100 -1.085705110344005 1104 1 -0.4727044505112623 1104 2 -0.3367653432800175 1104 3 -0.4090928379178891 1104 4 -0.24461810316775257 1104 5 -0.2195519285786105 1104 6 -0.4170341502278987 1104 7 -0.4201608517159059 1104 8 -0.42495692464618173 1104 9 -0.7272177026689945 1104 10-11 -0.5416668466061552 1104 12-13 -0.6464994844341447 1104 14-15 -0.7760716508984302 1104 16-17 -0.6846228982309768 1104 18-19 -0.7887233156322324 1104 20-21 -0.7517861194806414 1104 22-23 -0.7680017443476146 1104 24-25 -0.7520969002344984 1104 26-27 -0.9051924608238622 1104 28-29 -0.9006715904153779 1104 30-31 -0.8584546078604021 1104 32-33 -0.6398368388667635 1104 34-35 -0.7741203682573143 1104 36-37 -0.7685944472425064 1104 38-39 -0.8368109228403569 1104 40-41 -0.8195140281207429 1104 42-43 -0.7436083254255976 1104 44-45 -0.6820851289055625 1104 46-47 -0.6799546301454953 1104 48-49 -0.657723936987388 1104 50-51 -0.7475355033748059 1104 52-53 -0.7513972274815259 1104 54-55 -0.7955424194657255 1104 56-57 -0.9296300623187363 1104 58-59 -0.989426201526939 1104 60-61 -0.8287664172927478 1104 62-63 -0.9157086820219504 1104 64-65 -0.906198647995275 1104 66-67 -0.9196218683938682 1104 68-69 -0.9535667594257475 1104 70-71 -0.920346077528464 1104 72-73 -0.8260817097196664 1104 74-75 -0.6524363633156618 1104 76-77 -0.739983288547851 1104 78-79 -0.7045063825618847 1104 80-81 -0.8749949038457565 1104 82-83 -0.8618302058189045 1104 84-85 -0.8196551784879205 1104 86-87 -0.8925045906718516 1104 88-89 -0.8168278583505497 1104 90-91 -0.6660066254236341 1104 92-93 -0.8749623069474133 1104 94-95 -0.821566798493798 1104 96-97 -0.9640827257412958 1104 98-99 -1.0984281271809166 1104 100 -1.1671351390028697 1105 1 -0.2789987027689982 1105 2 -0.3091981037191118 1105 3 -0.3365100889311208 1105 4 -0.19643553734244534 1105 5 -0.20859368988406857 1105 6 -0.2516542048163757 1105 7 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-0.8179298063549041 1105 70-71 -0.900503143974511 1105 72-73 -0.9763093448585529 1105 74-75 -0.9461945322163672 1105 76-77 -1.0295616894391806 1105 78-79 -0.9721618647219223 1105 80-81 -0.7119349783341953 1105 82-83 -0.6999208176952614 1105 84-85 -0.5783439334011007 1105 86-87 -0.6579152650746956 1105 88-89 -0.7763463907551404 1105 90-91 -0.5763810322281167 1105 92-93 -0.6798162561437877 1105 94-95 -0.7735550229624479 1105 96-97 -0.7663223425344796 1105 98-99 -0.7859747203455534 1105 100 -0.8263631289839282 1106 1 -0.36050724310165094 1106 2 -0.28486059458095525 1106 3 -0.41599846975909927 1106 4 -0.25574978887486566 1106 5 -0.3287913077473661 1106 6 -0.3151106126563832 1106 7 -0.3429245338584366 1106 8 -0.5045387340397269 1106 9 -0.6251741173240575 1106 10-11 -0.5483240074744558 1106 12-13 -0.5559346066157929 1106 14-15 -0.6039507827054891 1106 16-17 -0.7200480655673331 1106 18-19 -0.6366848579092519 1106 20-21 -0.6894551751290265 1106 22-23 -0.6704901951003919 1106 24-25 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1.1576373575911063 2310 76-77 1.0173824874646442 2310 78-79 0.9523687178562277 2310 80-81 0.9466905511276984 2310 82-83 0.9537176193583043 2310 84-85 0.9406073471537297 2310 86-87 1.004301833098193 2310 88-89 0.9127220069490605 2310 90-91 0.9558730521921852 2310 92-93 0.9507473455218438 2310 94-95 1.0771137728899376 2310 96-97 1.1189193928473564 2310 98-99 1.1081856059068045 2310 100 1.178832704782046 2311 1 0.4930524044603679 2311 2 0.540803291297216 2311 3 0.5991733491966826 2311 4 0.41250843810077953 2311 5 0.04954840353492074 2311 6 0.15888676288752634 2311 7 0.35304667673701573 2311 8 0.1906225782764679 2311 9 0.4434866621534539 2311 10-11 0.562681091441263 2311 12-13 0.5607326867578806 2311 14-15 0.6851008112583372 2311 16-17 0.9141801089481092 2311 18-19 0.9027604939977536 2311 20-21 0.9475106759507952 2311 22-23 0.9567303621013536 2311 24-25 0.8870563865687089 2311 26-27 0.8595046858440796 2311 28-29 0.83156368624811 2311 30-31 0.9140159472223175 2311 32-33 1.1200342807753074 2311 34-35 1.0629234365209683 2311 36-37 1.121422775138953 2311 38-39 1.112540117752289 2311 40-41 1.1159953884441975 2311 42-43 1.1122514907392755 2311 44-45 1.1969091694634457 2311 46-47 1.2841491922399726 2311 48-49 1.2359278209289286 2311 50-51 1.2299325270949524 2311 52-53 1.198470952753155 2311 54-55 1.2015033224829779 2311 56-57 1.2572259729986328 2311 58-59 1.216526837306752 2311 60-61 1.2563196968092214 2311 62-63 1.248468852331733 2311 64-65 1.3440720657188834 2311 66-67 1.3875118202141792 2311 68-69 1.4083290837196358 2311 70-71 1.3622602389639198 2311 72-73 1.3299894833417554 2311 74-75 1.297930521253413 2311 76-77 1.2145734586211248 2311 78-79 1.1095534950247448 2311 80-81 1.1066696464183288 2311 82-83 1.052729678278613 2311 84-85 1.1547201828111895 2311 86-87 1.1742571202476064 2311 88-89 1.152029054545988 2311 90-91 1.2668677126427461 2311 92-93 1.3516836331662212 2311 94-95 1.4699229952955832 2311 96-97 1.5250297619703268 2311 98-99 1.5098252430054764 2311 100 1.589295235302643 2312 1 0.13166734935269986 2312 2 0.12403854281600246 2312 3 0.3225162399921828 2312 4 0.288537974338972 2312 5 0.3116564771047834 2312 6 0.4498880281052706 2312 7 0.482704486879463 2312 8 0.18831542283417235 2312 9 0.39254717502826253 2312 10-11 0.2859555455565115 2312 12-13 0.41720305206126085 2312 14-15 0.8804654743873925 2312 16-17 0.8619253678249166 2312 18-19 0.805580569445965 2312 20-21 0.8708700948502042 2312 22-23 0.6066003969717855 2312 24-25 0.3837650234284027 2312 26-27 0.7025071751745244 2312 28-29 0.9058521868843741 2312 30-31 0.7845445624706642 2312 32-33 0.6723728460713332 2312 34-35 0.7731709793105779 2312 36-37 0.8567634983424881 2312 38-39 0.9342038672516537 2312 40-41 0.898689774979303 2312 42-43 0.7745989528037285 2312 44-45 0.7348065729800979 2312 46-47 0.8854982664401874 2312 48-49 0.9414233186346834 2312 50-51 1.0022010265733954 2312 52-53 1.0225221309078307 2312 54-55 0.942413349554208 2312 56-57 0.9965391441972926 2312 58-59 0.959778022950708 2312 60-61 1.0453630919055783 2312 62-63 1.0155663472694627 2312 64-65 1.1673976106136692 2312 66-67 1.1288001321115573 2312 68-69 1.1402237080184037 2312 70-71 1.0669913656662615 2312 72-73 1.159737799917778 2312 74-75 1.142723232359593 2312 76-77 1.1134233995783447 2312 78-79 1.0822652834150865 2312 80-81 0.9714087444874444 2312 82-83 0.9405452267910626 2312 84-85 0.9445604993760313 2312 86-87 1.0625252009279968 2312 88-89 0.7831494297167261 2312 90-91 1.1051529132536793 2312 92-93 1.207757474622536 2312 94-95 1.2384683133980978 2312 96-97 1.3598758041888743 2312 98-99 1.4898197271812137 2312 100 1.57400437187923 2313 1 -0.38319505050014513 2313 2 -0.035255470836709435 2313 3 0.22056562718697137 2313 4 0.16841312251588647 2313 5 0.25946435296318526 2313 6 0.36275020995070406 2313 7 0.11350254729476461 2313 8 0.06891506244066647 2313 9 0.2679708417766804 2313 10-11 0.37971299698173766 2313 12-13 0.5723118130638127 2313 14-15 0.629351592765417 2313 16-17 0.7958235617697582 2313 18-19 0.6262679825836344 2313 20-21 0.8066525413362058 2313 22-23 0.9316821284615955 2313 24-25 0.6674472750702733 2313 26-27 0.9351926177295624 2313 28-29 0.7825599470966651 2313 30-31 0.8812843936171078 2313 32-33 1.0066021600633164 2313 34-35 1.0705032839214326 2313 36-37 0.8843780544249 2313 38-39 0.7654042833392367 2313 40-41 0.8601072532126253 2313 42-43 0.8035229371957584 2313 44-45 0.9140551840344386 2313 46-47 0.9903587672440892 2313 48-49 1.0351221166985098 2313 50-51 0.9626919733046506 2313 52-53 0.8830721142166311 2313 54-55 0.9652127946572122 2313 56-57 1.0423488032881991 2313 58-59 0.9211243932976174 2313 60-61 0.9880995277569014 2313 62-63 1.1271331307744745 2313 64-65 1.2450636028199042 2313 66-67 1.1521580083106002 2313 68-69 1.110073431932456 2313 70-71 1.0228745639371866 2313 72-73 1.112202710091914 2313 74-75 0.9404643008684204 2313 76-77 0.9671355293063826 2313 78-79 0.8881948295509012 2313 80-81 0.874089831152201 2313 82-83 0.9926456984024163 2313 84-85 1.0721397569926694 2313 86-87 1.1638778900834872 2313 88-89 1.1085871264343545 2313 90-91 1.2175635174407873 2313 92-93 0.958682941635292 2313 94-95 0.9688347659746732 2313 96-97 1.3199713995875548 2313 98-99 1.3296950925139477 2313 100 1.5997279691377884 2314 1 -0.3696281419108942 2314 2 -0.2336957199569909 2314 3 0.08005496559868419 2314 4 -0.16310266743133184 2314 5 0.18061895848941845 2314 6 0.2524942418604894 2314 7 0.1552479387694916 2314 8 0.21189056028136122 2314 9 0.28114287672549665 2314 10-11 0.43234427997117564 2314 12-13 0.5000702428662791 2314 14-15 0.6118272005709144 2314 16-17 0.7656269746776303 2314 18-19 0.747023715749016 2314 20-21 0.5061458909643193 2314 22-23 0.6319471061687238 2314 24-25 0.8403066475710688 2314 26-27 0.8059039900904139 2314 28-29 0.6912407317455731 2314 30-31 0.6966312951086238 2314 32-33 0.7475297507343015 2314 34-35 0.7653684270756216 2314 36-37 0.7827430776174822 2314 38-39 0.7154268386215534 2314 40-41 0.7609799114619804 2314 42-43 0.5786183441655197 2314 44-45 0.6285849720022938 2314 46-47 0.7372928733301407 2314 48-49 0.616391423020481 2314 50-51 0.753679794701803 2314 52-53 0.8368775214863149 2314 54-55 0.8833171262710948 2314 56-57 0.7124118831026536 2314 58-59 0.7524480572251591 2314 60-61 0.8781222204952215 2314 62-63 0.6215405454017926 2314 64-65 0.7171900223656493 2314 66-67 0.8299098777123888 2314 68-69 0.9567790486351058 2314 70-71 0.9491359727863582 2314 72-73 0.9601241877190745 2314 74-75 0.919958362768611 2314 76-77 0.7419319694455311 2314 78-79 0.720197338747969 2314 80-81 0.6636093723990051 2314 82-83 0.7912348373779459 2314 84-85 0.67666443409599 2314 86-87 0.7886738928625974 2314 88-89 0.8859424352355383 2314 90-91 0.9920491195481098 2314 92-93 1.0581442390201268 2314 94-95 1.0827611220407292 2314 96-97 1.148997673679844 2314 98-99 1.2257580398707937 2314 100 1.1469765995760461 2315 1 -0.007150927072473223 2315 2 0.1551066557356151 2315 3 0.13991384849200728 2315 4 -0.05474307834889203 2315 5 0.27300479124934895 2315 6 0.3513287280324633 2315 7 0.3669592100306147 2315 8 0.3434065129123667 2315 9 0.596697748223221 2315 10-11 0.560074877877959 2315 12-13 0.6922318454930618 2315 14-15 0.7670677877339713 2315 16-17 0.73378297607794 2315 18-19 0.8146217070333179 2315 20-21 0.7721344350075867 2315 22-23 0.9106307575889474 2315 24-25 0.8752198527039141 2315 26-27 0.859465416471707 2315 28-29 0.8783373891170996 2315 30-31 0.8877462892538972 2315 32-33 0.889708857193817 2315 34-35 0.8783010433553997 2315 36-37 0.9477674019091751 2315 38-39 0.9408888794477264 2315 40-41 0.8968229322613652 2315 42-43 0.9935847247350651 2315 44-45 0.7949483394982835 2315 46-47 0.7511550034862253 2315 48-49 0.8777622749139127 2315 50-51 0.86324413034081 2315 52-53 0.8279436256160082 2315 54-55 0.7914669711509177 2315 56-57 0.8466677891372569 2315 58-59 0.9189028671576764 2315 60-61 1.0937353375053291 2315 62-63 1.117081293117245 2315 64-65 1.0591310163419152 2315 66-67 1.0915619980904694 2315 68-69 1.0035022104308808 2315 70-71 0.6845054847312113 2315 72-73 0.9143112252255676 2315 74-75 1.2012494816860269 2315 76-77 1.0735906688504073 2315 78-79 0.8937544797081394 2315 80-81 0.9879502625594334 2315 82-83 0.9545259804385786 2315 84-85 0.9193058831719085 2315 86-87 0.9718101571825422 2315 88-89 1.0538781297755513 2315 90-91 1.0410176005775789 2315 92-93 1.1009257187579315 2315 94-95 1.156969224504543 2315 96-97 1.2071010428658582 2315 98-99 1.1888729374431932 2315 100 1.0004821947667182 2316 1 0.5308112268778267 2316 2 0.46758254934476895 2316 3 0.4799332123194304 2316 4 0.3026348547593187 2316 5 0.38853560413652843 2316 6 0.4196972074997234 2316 7 0.45311407342117604 2316 8 0.524581487920436 2316 9 0.4758568385956252 2316 10-11 0.41994354232486586 2316 12-13 0.5810057452577055 2316 14-15 0.7248420495864423 2316 16-17 0.7252380968657164 2316 18-19 0.8129991993389538 2316 20-21 0.8035955381160278 2316 22-23 0.805752214441469 2316 24-25 0.9779479090007044 2316 26-27 1.0226782768868432 2316 28-29 0.8727970517456995 2316 30-31 0.8534140657984821 2316 32-33 0.8223111273270831 2316 34-35 0.7550650408050359 2316 36-37 0.6192077243247738 2316 38-39 0.6215760198197415 2316 40-41 0.7456520504200697 2316 42-43 0.8409747405567316 2316 44-45 0.710310309256073 2316 46-47 0.7394930203469201 2316 48-49 0.484027771879461 2316 50-51 0.5678341839455499 2316 52-53 0.7380173419752936 2316 54-55 0.766244601994508 2316 56-57 0.8367940082318768 2316 58-59 0.8675273269777364 2316 60-61 0.8784784519329563 2316 62-63 0.813420270297847 2316 64-65 0.9128922036751348 2316 66-67 0.8723689816627029 2316 68-69 0.8341985997855019 2316 70-71 0.7633148848461317 2316 72-73 0.8019057813137564 2316 74-75 0.8978671090661692 2316 76-77 0.7546111661701644 2316 78-79 0.6854422025692664 2316 80-81 0.6513213396133004 2316 82-83 0.6741243778063613 2316 84-85 0.6874185397158605 2316 86-87 0.6145333725224731 2316 88-89 0.6012201296023783 2316 90-91 0.6938777008601633 2316 92-93 0.7525084755057527 2316 94-95 0.6545158826700188 2316 96-97 0.8301083790801478 2316 98-99 0.8634207212295308 2316 100 0.7095568242170529 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 18.0 13 89.0 14 431.0 15 1195.0 16 2721.0 17 5539.0 18 8380.0 19 11399.0 20 14748.0 21 18990.0 22 22260.0 23 22845.0 24 23592.0 25 25755.0 26 29347.0 27 35988.0 28 43548.0 29 53125.0 30 64622.0 31 79990.0 32 99802.0 33 127975.0 34 175820.0 35 241179.0 36 359266.0 37 546579.0 38 488470.0 39 70922.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.45128774695386 19.5456025716855 13.033419942221016 23.96968973913963 2 16.203423905190856 21.387250233433956 39.130872165195115 23.278453696180073 3 18.732783760143306 26.34541078938742 31.063379067227427 23.858426383241852 4 12.791480504385344 17.535156360777158 38.300902475115016 31.372460659722478 5 12.735339894294901 37.61981354978266 34.87029237671784 14.774554179204596 6 29.440305942314215 37.3620321268949 18.77637057053068 14.421291360260202 7 25.92590147048142 33.41300917152535 22.809338463970505 17.851750894022718 8 25.413511639694786 35.10478347079832 21.025442836640327 18.456262052866567 9 26.525730874446946 16.005846451791825 20.752184942395825 36.71623773136541 10-11 24.440507677246288 26.022100230774743 29.163553709651076 20.373838382327893 12-13 25.052969537321047 24.32533996635585 28.558475067709733 22.06321542861338 14-15 22.669529502309775 25.865846915845488 26.607048425126433 24.857575156718305 16-17 21.73352298084247 28.29879270613194 27.47023713577366 22.49744717725193 18-19 21.295954640014543 27.576648680729154 29.635256528771027 21.492140150485277 20-21 22.15566396922866 27.081237945959447 29.374095855925 21.389002228886895 22-23 22.107792520285162 26.897695120821517 29.077720122729577 21.916792236163744 24-25 21.723757021956395 26.7626698174437 29.451297928263116 22.06227523233679 26-27 21.751543490094686 26.9105708898477 29.267788224478057 22.070097395579552 28-29 21.491225344464755 27.613908165001277 29.212483307918124 21.68238318261584 30-31 22.380517544006157 27.000935685507127 29.291131806587707 21.32741496389901 32-33 22.164198252405047 27.090363365884894 28.936861931374647 21.808576450335412 34-35 22.215017691353626 27.077318451163997 29.233730801991843 21.473933055490534 36-37 21.999697334033467 26.856165090103346 29.19246272381109 21.951674852052097 38-39 21.834451406176672 26.973546194512043 29.33470780926097 21.857294590050316 40-41 21.847516984667337 26.403794917388186 29.75495431040493 21.993733787539547 42-43 21.496925595348493 26.737017750776005 29.302404077429046 22.463652576446457 44-45 21.107402728768605 27.058521225568573 29.562495047289644 22.27158099837318 46-47 21.06394814473542 26.503335739219548 29.28871877624559 23.143997339799444 48-49 21.16269268641324 26.578576824997914 29.415351987119603 22.843378501469243 50-51 21.042234372116965 26.830897419711963 29.13480232682354 22.992065881347536 52-53 20.7793643113658 27.13529890154244 28.8208707907562 23.264465996335563 54-55 20.56185855142407 26.79928012868496 29.126140239726627 23.512721080164344 56-57 20.92201157262918 26.620018543240825 28.684206744317297 23.773763139812694 58-59 20.52814259585947 26.752746922997588 28.822759494954227 23.896350986188715 60-61 20.98532622195223 26.828114412626476 28.401335607639133 23.78522375778216 62-63 20.86399773788214 26.68891237924965 28.248390950207963 24.19869893266024 64-65 20.503925237130318 26.395665767456894 28.172161895053517 24.92824710035927 66-67 20.66954984795587 26.47866340280027 27.79771981407697 25.05406693516689 68-69 20.606530129087744 26.603512109966886 27.674042276362105 25.11591548458327 70-71 20.750703110200078 26.287296413773674 27.5883830165492 25.37361745947705 72-73 20.869451519076637 26.462574024592843 27.21921265674096 25.448761799589555 74-75 21.16820565380113 26.42655307417326 27.002803939112912 25.402437332912697 76-77 21.091785384388995 26.1963803357412 27.09050520473647 25.621329075133335 78-79 21.038539686128313 26.11440881166825 27.090919226180176 25.75613227602327 80-81 20.989405318567755 26.301991438103762 26.827404360340743 25.881198882987743 82-83 21.299020031590075 25.890770440841067 27.022628417976623 25.78758110959224 84-85 21.50098215794965 25.871478172001016 26.70494265402199 25.922597016027343 86-87 21.13076525516925 25.962038400708792 26.956874006660474 25.95032233746148 88-89 21.189311809114407 25.423093492425387 27.03546673149116 26.352127966969046 90-91 21.529513402873388 25.12174496026278 26.912518278499935 26.436223358363893 92-93 21.534780548744205 25.041847461752575 26.932457215741756 26.49091477376146 94-95 21.13551459499248 24.78879284811866 27.30778007926034 26.76791247762852 96-97 21.162346312360906 24.66211393709729 27.398742940132635 26.77679681040917 98-99 21.129997672903468 24.773690833376392 27.188995591725483 26.90731590199466 100 21.08638274629366 24.794044680436127 27.34596375745794 26.77360881581227 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1859.0 1 1259.5 2 1337.0 3 2084.0 4 1519.0 5 923.5 6 1212.5 7 1965.5 8 2886.0 9 3251.5 10 2740.5 11 2142.0 12 2057.0 13 2264.5 14 2495.0 15 2750.0 16 3020.5 17 3269.0 18 3632.5 19 3965.5 20 4378.5 21 4903.0 22 5511.0 23 6405.0 24 7560.5 25 8844.5 26 10673.5 27 13088.5 28 15718.0 29 19371.5 30 24264.0 31 28944.5 32 34477.5 33 40272.5 34 45689.5 35 51030.5 36 56039.0 37 62406.0 38 66659.0 39 68567.0 40 70796.0 41 73020.5 42 76095.5 43 81224.0 44 87593.5 45 94049.5 46 100513.5 47 105696.5 48 109396.0 49 112063.5 50 113981.5 51 114502.5 52 112570.5 53 108911.0 54 103408.5 55 92912.5 56 81725.5 57 70820.5 58 58723.5 59 48163.0 60 37213.5 61 27516.5 62 20602.5 63 15241.0 64 10780.5 65 7401.0 66 5244.5 67 3880.0 68 2807.5 69 1945.5 70 1420.5 71 1100.5 72 836.5 73 632.0 74 456.5 75 315.5 76 221.5 77 132.0 78 74.5 79 43.5 80 28.0 81 13.0 82 5.5 83 5.0 84 3.0 85 2.0 86 0.5 87 1.0 88 1.0 89 1.0 90 1.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.052435248294494995 2 0.0 3 0.0 4 0.017905666269453478 5 0.0032237967469948775 6 3.884092466258889E-5 7 1.9420462331294446E-4 8 3.495683219633E-4 9 0.0019808871577920335 10-11 6.020343322701277E-4 12-13 4.272501712884778E-4 14-15 1.9420462331294444E-5 16-17 1.1652277398776665E-4 18-19 0.0 20-21 1.9420462331294444E-5 22-23 1.9420462331294444E-5 24-25 3.884092466258889E-5 26-27 1.9420462331294444E-5 28-29 0.0011652277398776665 30-31 8.156594179143666E-4 32-33 0.014778971834115072 34-35 0.02355702080786016 36-37 0.031441728514365705 38-39 0.02066337192049729 40-41 9.710231165647223E-5 42-43 0.00157305744883485 44-45 0.009943276713622756 46-47 0.014060414727857178 48-49 0.005787297774725744 50-51 0.010836617980862301 52-53 0.015672313101354616 54-55 0.009904435788960166 56-57 0.0031461148976697 58-59 0.008680946662088617 60-61 0.0017672620721477944 62-63 9.904435788960168E-4 64-65 0.019109734933993733 66-67 0.04557982509154806 68-69 0.05410540805498632 70-71 0.04724998485203938 72-73 0.040802391358049625 74-75 0.028159670380376943 76-77 0.028994750260622603 78-79 0.013031130224298571 80-81 0.002233353168098861 82-83 0.026975022178167978 84-85 0.04618185942381819 86-87 0.047152882540382905 88-89 0.0583584893055398 90-91 0.034315956939397284 92-93 0.026120521835591028 94-95 0.006855423202946939 96-97 0.004058876627240539 98-99 0.022566577228964144 100 0.019498144180619622 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2574604.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.21812022973863 #Duplication Level Percentage of deduplicated Percentage of total 1 83.21244937874036 60.094466738449114 2 11.193419808002284 16.167354749524936 3 2.762117551015868 5.984248121638056 4 1.028304054659077 2.9704874340838785 5 0.5371850857526583 1.9397248554253967 6 0.3127307120102241 1.3550894495691683 7 0.22012543538078808 1.1127931610567323 8 0.14249249735799935 0.823243224482857 9 0.10584591001586997 0.6879593389816976 >10 0.4620286287677795 5.472026270191834 >50 0.01530584621926153 0.7552093716989774 >100 0.006969624225077673 0.9228459562339795 >500 5.127339263725569E-4 0.22719152537790857 >1k 3.9879305383413516E-4 0.7209895491901778 >5k 1.1394087252403861E-4 0.766370254095266 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9933 0.3858069046734954 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8694 0.3376829990165478 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4835 0.18779587074361725 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3337 0.1296121655990591 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2619 0.10172438169132031 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1652277398776667E-4 3.884092466258889E-5 10-11 0.0 0.0 0.0 1.1652277398776667E-4 5.826138699388333E-5 12-13 0.0 0.0 0.0 1.7478416098164998E-4 1.1652277398776667E-4 14-15 0.0 0.0 0.0 2.1362508564423888E-4 1.1652277398776667E-4 16-17 3.884092466258889E-5 0.0 0.0 3.495683219633E-4 1.1652277398776667E-4 18-19 3.884092466258889E-5 0.0 0.0 5.631934076075389E-4 1.5536369865035556E-4 20-21 3.884092466258889E-5 0.0 0.0 0.001359432363190611 2.330455479755333E-4 22-23 3.884092466258889E-5 0.0 0.0 0.0052823657541120886 3.4956832196329996E-4 24-25 3.884092466258889E-5 0.0 0.0 0.015633472176692028 3.8840924662588886E-4 26-27 3.884092466258889E-5 0.0 0.0 0.0307620123327704 3.8840924662588886E-4 28-29 3.884092466258889E-5 0.0 0.0 0.07313746113965487 3.8840924662588886E-4 30-31 3.884092466258889E-5 0.0 0.0 0.19484549856987715 3.8840924662588886E-4 32-33 3.884092466258889E-5 0.0 0.0 0.45335127266173747 4.6609109595106667E-4 34-35 3.884092466258889E-5 0.0 0.0 0.8221846932576815 4.855115582823611E-4 36-37 3.884092466258889E-5 0.0 0.0 1.347158630997233 5.049320206136556E-4 38-39 3.884092466258889E-5 0.0 0.0 2.0565492790347566 5.049320206136556E-4 40-41 3.884092466258889E-5 0.0 0.0 2.818200391205793 5.049320206136556E-4 42-43 3.884092466258889E-5 0.0 0.0 3.54279726124872 5.049320206136556E-4 44-45 3.884092466258889E-5 0.0 0.0 4.377838300569719 5.049320206136556E-4 46-47 3.884092466258889E-5 0.0 0.0 5.322779736223513 5.049320206136556E-4 48-49 3.884092466258889E-5 0.0 0.0 6.2500291306934965 5.437729452762444E-4 50-51 3.884092466258889E-5 0.0 0.0 7.168267430641761 5.437729452762444E-4 52-53 3.884092466258889E-5 0.0 0.0 8.032342061148045 5.437729452762444E-4 54-55 3.884092466258889E-5 1.9420462331294444E-5 0.0 8.963922218717908 5.631934076075389E-4 56-57 3.884092466258889E-5 3.884092466258889E-5 0.0 10.001460418767312 5.826138699388333E-4 58-59 3.884092466258889E-5 3.884092466258889E-5 0.0 11.090307480295998 5.826138699388333E-4 60-61 3.884092466258889E-5 3.884092466258889E-5 0.0 12.142974220501483 5.826138699388333E-4 62-63 3.884092466258889E-5 3.884092466258889E-5 0.0 13.180609522862547 6.602957192640111E-4 64-65 3.884092466258889E-5 3.884092466258889E-5 0.0 14.209505617174525 6.991366439266E-4 66-67 3.884092466258889E-5 7.768184932517778E-5 0.0 15.310898297369226 7.185571062578945E-4 68-69 3.884092466258889E-5 7.768184932517778E-5 0.0 16.483214505997815 7.379775685891889E-4 70-71 3.884092466258889E-5 7.768184932517778E-5 0.0 17.66990185675156 7.379775685891889E-4 72-73 3.884092466258889E-5 7.768184932517778E-5 0.0 18.780713461176944 8.156594179143667E-4 74-75 3.884092466258889E-5 7.768184932517778E-5 0.0 19.84526552432918 8.156594179143667E-4 76-77 3.884092466258889E-5 7.768184932517778E-5 0.0 20.960504994166094 8.156594179143667E-4 78-79 3.884092466258889E-5 7.768184932517778E-5 0.0 22.136744136185605 8.156594179143667E-4 80-81 3.884092466258889E-5 7.768184932517778E-5 0.0 23.28002675362891 8.350798802456612E-4 82-83 3.884092466258889E-5 7.768184932517778E-5 0.0 24.396994644613308 9.321821919021333E-4 84-85 3.884092466258889E-5 7.768184932517778E-5 0.0 25.483297625576594 9.321821919021333E-4 86-87 3.884092466258889E-5 7.768184932517778E-5 0.0 26.56930929960491 9.321821919021333E-4 88 3.884092466258889E-5 7.768184932517778E-5 0.0 27.395591710414493 9.321821919021333E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4415 0.0 53.989677 1 TACCTGG 2145 0.0 42.723183 2 GTATCAA 7565 0.0 39.09086 1 ACCTGGG 2575 0.0 34.676292 3 ATCAACG 9220 0.0 31.602182 3 CAACGCA 9355 0.0 31.240541 5 TATCAAC 9520 0.0 30.85314 2 TCAACGC 9695 0.0 30.10233 4 GTGGTAT 1750 0.0 30.089396 1 AACGCAG 10170 0.0 29.477356 6 CCTGGGG 2495 0.0 27.123657 4 CTGGGGG 1655 0.0 26.687126 5 GTACATG 7160 0.0 26.265184 1 ACGCAGA 11235 0.0 26.01394 7 TGGGGGG 4505 0.0 25.971281 6 TGGTATC 1990 0.0 25.741255 2 CGCAGAG 11405 0.0 25.378984 8 TACATGG 7330 0.0 25.260885 2 ACATGGG 7380 0.0 23.625113 3 CATGGGG 5595 0.0 23.434755 4 >>END_MODULE