FastQCFastQC Report
Mon 6 Jun 2016
ERR1211448_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211448_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230907
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45350.368427509145695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36900.29977894349451256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19010.15443896248863642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22550.052.0336271
GTATCAA42000.041.5129661
CGCCGTT802.4586552E-741.12523394
ATCAACG47300.036.2716523
CAACGCA47850.035.9529655
TATCAAC48700.035.4680982
AACGCAG50800.034.512846
TCAACGC50850.034.0167244
ACGCAGA56550.030.3386217
CGCAGAG56750.030.0648278
TACCTGG7600.029.725452
TACCGAC2600.028.9255897
TATACCG2750.027.3478325
ATACCGA2750.027.3478326
GTGGTAT9350.027.215321
GCAGAGT61150.026.9791729
GTACATG40300.026.192341
GTACTAG5850.024.971071
TACATGG41050.024.6505222
ACATGGG40000.023.9720823
Produced by FastQC (version 0.11.5)