FastQCFastQC Report
Mon 6 Jun 2016
ERR1211448_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211448_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230907
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39490.3208203381733957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35540.28873018026544656No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT19550.15882597141782442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19150.15557633517398148No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21600.053.109241
GTATCAA35500.036.684741
CTGCGCG550.002722113434.1689229
ATACCGA2350.029.9886826
CAACGCA43350.029.9138035
ATCAACG43500.029.8142833
GTGGTAT7950.029.5690171
TATCAAC44450.029.3885142
AACGCAG46650.028.199436
TCAACGC46150.028.1023044
ATGGGCG1205.8589394E-627.4074365
TATACCG2400.027.4074365
GTACTAG5650.026.6278151
CGTTAAC1606.895789E-826.445791
GTACATG38600.025.6997661
TACCGAC2208.913048E-1125.6266947
TACCTGG7350.025.5727392
ACGCAGA51900.025.0753157
CGCAGAG53300.024.2403838
TACATGG40850.024.0413442
Produced by FastQC (version 0.11.5)