##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211448_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1230907 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.078668819009074 33.0 31.0 34.0 30.0 34.0 2 32.21407141238128 33.0 31.0 34.0 30.0 34.0 3 32.296079232630895 34.0 31.0 34.0 30.0 34.0 4 35.932915321791164 37.0 35.0 37.0 35.0 37.0 5 35.792552158692736 37.0 35.0 37.0 35.0 37.0 6 35.82643286617105 37.0 35.0 37.0 35.0 37.0 7 35.75862351907983 37.0 35.0 37.0 33.0 37.0 8 35.745476303246306 37.0 35.0 37.0 33.0 37.0 9 37.33802878690267 39.0 37.0 39.0 34.0 39.0 10-11 37.31978817246144 39.0 37.0 39.0 34.0 39.0 12-13 37.2372803144348 39.0 37.0 39.0 33.0 39.0 14-15 38.45228193519088 40.0 38.0 41.0 34.0 41.0 16-17 38.440320024177296 40.0 38.0 41.0 34.0 41.0 18-19 38.44832428445041 40.0 38.0 41.0 34.0 41.0 20-21 38.4094886941093 40.0 38.0 41.0 34.0 41.0 22-23 38.310334980628106 40.0 38.0 41.0 34.0 41.0 24-25 38.189413984972056 40.0 37.5 41.0 33.0 41.0 26-27 38.05634747385464 40.0 37.0 41.0 33.0 41.0 28-29 37.98123822514617 40.0 37.0 41.0 33.0 41.0 30-31 38.02444335762166 40.0 37.0 41.0 33.0 41.0 32-33 37.86394626076543 40.0 37.0 41.0 33.0 41.0 34-35 37.746265152444494 40.0 37.0 41.0 33.0 41.0 36-37 37.5952236846488 40.0 37.0 41.0 32.5 41.0 38-39 37.48069147384815 40.0 36.0 41.0 32.0 41.0 40-41 37.41616019731791 40.0 36.0 41.0 32.0 41.0 42-43 37.279285112522714 40.0 36.0 41.0 31.5 41.0 44-45 37.1277622923584 40.0 36.0 41.0 31.0 41.0 46-47 36.902828970832076 39.0 36.0 41.0 31.0 41.0 48-49 36.69450738357975 39.0 35.5 41.0 30.0 41.0 50-51 36.51793636724789 39.0 35.0 41.0 30.0 41.0 52-53 36.31888964804003 39.0 35.0 40.5 29.5 41.0 54-55 36.12486077339718 39.0 35.0 40.0 29.0 41.0 56-57 35.885739946234764 38.0 35.0 40.0 28.0 41.0 58-59 35.61020044568761 38.0 34.5 40.0 28.0 41.0 60-61 35.34507359207478 38.0 34.0 40.0 27.0 41.0 62-63 35.040965726898946 37.0 34.0 40.0 26.5 41.0 64-65 34.688912322376915 37.0 34.0 40.0 26.0 41.0 66-67 34.3050295432555 36.0 33.0 39.0 26.0 41.0 68-69 33.89480846237774 36.0 33.0 39.0 25.5 40.0 70-71 33.51129532937907 35.0 32.5 38.5 25.0 40.0 72-73 33.13852346277989 35.0 32.0 37.5 24.0 40.0 74-75 32.75193536148548 35.0 32.0 37.0 24.0 39.0 76-77 31.68145156376558 34.0 30.5 36.0 22.5 38.5 78-79 32.12698684790971 35.0 31.0 36.0 23.0 38.5 80-81 31.985459908831455 35.0 31.0 35.5 23.0 37.5 82-83 31.708887430163287 34.5 31.0 35.0 23.0 37.0 84-85 31.42667845743017 34.0 31.0 35.0 22.5 36.5 86-87 31.15453401434877 34.0 31.0 35.0 20.5 36.0 88-89 30.901076198283057 34.0 30.0 35.0 20.0 36.0 90-91 30.68287206100867 34.0 30.0 35.0 20.0 35.0 92-93 30.509265525340258 34.0 30.0 35.0 19.0 35.0 94-95 30.339548804255724 34.0 30.0 35.0 19.0 35.0 96-97 30.16118073908102 34.0 29.0 35.0 18.0 35.0 98-99 29.93065926182888 34.0 29.0 35.0 18.0 35.0 100 27.9646561438029 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.31943228112368516 1101 2 -0.22438196823995327 1101 3 -0.3375186280744771 1101 4 -0.08648308672485427 1101 5 -0.2548125155619232 1101 6 -0.38439099092960305 1101 7 -0.35904168783756774 1101 8 -0.4284972706896255 1101 9 -0.46663855234507423 1101 10-11 -0.48800937668418953 1101 12-13 -0.5004019737444807 1101 14-15 -0.638294181383877 1101 16-17 -0.5967442992289378 1101 18-19 -0.6434963783928325 1101 20-21 -0.6042345689321493 1101 22-23 -0.7805484305336492 1101 24-25 -0.7901253916366784 1101 26-27 -0.6671570744644839 1101 28-29 -0.8660263633443819 1101 30-31 -0.8419898023733552 1101 32-33 -0.6540170291544882 1101 34-35 -0.754314915584871 1101 36-37 -0.6254668471387745 1101 38-39 -0.6029790303983944 1101 40-41 -0.6034475692185808 1101 42-43 -0.6236557224995849 1101 44-45 -0.8169469665985503 1101 46-47 -0.732652458551847 1101 48-49 -0.7285664209219433 1101 50-51 -0.8683637702343177 1101 52-53 -0.8626656117622602 1101 54-55 -0.801990216748095 1101 56-57 -0.79065074305867 1101 58-59 -0.6930504728880607 1101 60-61 -0.5527416603734281 1101 62-63 -0.7931933799740563 1101 64-65 -0.8182129336168202 1101 66-67 -0.7645908951269149 1101 68-69 -0.9413130360876067 1101 70-71 -0.9751926853043571 1101 72-73 -0.9368800596175149 1101 74-75 -0.7385356765165199 1101 76-77 -0.6241249813089951 1101 78-79 -0.830287198129458 1101 80-81 -0.7827527524517848 1101 82-83 -0.9623997529317627 1101 84-85 -1.1040463029954886 1101 86-87 -1.4658348523248925 1101 88-89 -1.3457325254864543 1101 90-91 -1.4805004200424179 1101 92-93 -1.8721673100305622 1101 94-95 -1.7933345208411566 1101 96-97 -1.6763977980716653 1101 98-99 -1.82696752868846 1101 100 -2.0844728026054646 1102 1 -0.28930066325914794 1102 2 -0.24878303301369087 1102 3 -0.365986536977168 1102 4 -0.2231660207680406 1102 5 -0.25037595833033777 1102 6 -0.3560709672679607 1102 7 -0.2771872069147676 1102 8 -0.39570371848613206 1102 9 -0.5328541690265283 1102 10-11 -0.5817316482014903 1102 12-13 -0.646937761972822 1102 14-15 -0.7361942109609174 1102 16-17 -0.6573587624055008 1102 18-19 -0.9134608859349882 1102 20-21 -0.901021762070279 1102 22-23 -0.8557295899539383 1102 24-25 -0.9574945131983483 1102 26-27 -0.885084462811129 1102 28-29 -0.668340767366864 1102 30-31 -0.7693411777060959 1102 32-33 -0.8281703861494663 1102 34-35 -0.9958484855345802 1102 36-37 -0.9571179858551275 1102 38-39 -0.9538552504516318 1102 40-41 -1.008301902256143 1102 42-43 -0.9136956515146721 1102 44-45 -0.7701597734604277 1102 46-47 -0.915789843940793 1102 48-49 -1.0040026823830388 1102 50-51 -1.0422213567471843 1102 52-53 -1.0098668835753344 1102 54-55 -1.1058204444913642 1102 56-57 -1.0136616865665076 1102 58-59 -1.2131258943609993 1102 60-61 -1.391195520178215 1102 62-63 -1.160762146611141 1102 64-65 -1.3054208602657411 1102 66-67 -1.1804257073126578 1102 68-69 -1.3873903800686733 1102 70-71 -1.3920996654877342 1102 72-73 -1.1058019762102376 1102 74-75 -1.1367351737066969 1102 76-77 -1.0663757946778212 1102 78-79 -1.1946764320839094 1102 80-81 -0.9679235599052021 1102 82-83 -0.8223524296546252 1102 84-85 -0.9815973234036548 1102 86-87 -1.300018525794279 1102 88-89 -1.43764320280086 1102 90-91 -1.364133220988883 1102 92-93 -1.353958939725917 1102 94-95 -1.4638646894303342 1102 96-97 -1.4288933615144366 1102 98-99 -1.6314262687062069 1102 100 -1.6697286440724852 1103 1 -0.33350927196953606 1103 2 -0.19336869646056343 1103 3 -0.3412691767234577 1103 4 -0.20437078025190658 1103 5 -0.2205444527743765 1103 6 -0.3406135387449538 1103 7 -0.17480141273944128 1103 8 -0.2498295325128126 1103 9 -0.5463173834938075 1103 10-11 -0.4703582732287259 1103 12-13 -0.6084621404074753 1103 14-15 -0.6749999823798092 1103 16-17 -0.6987515650781759 1103 18-19 -0.5285473119644664 1103 20-21 -0.5319605254759949 1103 22-23 -0.7312848832456353 1103 24-25 -0.6251174342528927 1103 26-27 -0.6614313894821535 1103 28-29 -0.848567484388191 1103 30-31 -0.7385110520615328 1103 32-33 -0.6283177582655668 1103 34-35 -0.594442549494687 1103 36-37 -0.5946536940885991 1103 38-39 -0.8646376238992275 1103 40-41 -0.8884569001983849 1103 42-43 -0.7377841837247061 1103 44-45 -0.8034758579013186 1103 46-47 -0.7880466648186015 1103 48-49 -0.9054994020673561 1103 50-51 -0.7672513153498457 1103 52-53 -0.7954525591266943 1103 54-55 -0.6937232212604343 1103 56-57 -0.9214194515942253 1103 58-59 -0.8083034671694804 1103 60-61 -0.8587176713874527 1103 62-63 -0.7349686975589265 1103 64-65 -0.7640991714478602 1103 66-67 -0.876825530587162 1103 68-69 -0.8905602423196228 1103 70-71 -0.7017641896603948 1103 72-73 -0.9410478183193263 1103 74-75 -1.1266385193866064 1103 76-77 -0.871088071047442 1103 78-79 -0.9106749311276516 1103 80-81 -0.9141387897693889 1103 82-83 -1.0985404849479217 1103 84-85 -1.0415871556354759 1103 86-87 -0.9179646366229512 1103 88-89 -0.9406148540948323 1103 90-91 -1.3752103386686976 1103 92-93 -1.3418317578923329 1103 94-95 -1.420719762420383 1103 96-97 -1.398231185612044 1103 98-99 -1.4297776377235643 1103 100 -1.5183862565836819 1104 1 -0.45319528247867424 1104 2 -0.3307840644732032 1104 3 -0.3712420687927853 1104 4 -0.24641376752016697 1104 5 -0.3210493540424082 1104 6 -0.2989784551728718 1104 7 -0.34832107562470327 1104 8 -0.3894996122949195 1104 9 -0.5862598273187274 1104 10-11 -0.6244543329432588 1104 12-13 -0.5532698796574422 1104 14-15 -0.8217746525238212 1104 16-17 -0.9019389952206538 1104 18-19 -0.8651112211593315 1104 20-21 -0.7575184221061306 1104 22-23 -0.8074520659479418 1104 24-25 -0.7457782382407103 1104 26-27 -0.873000116437197 1104 28-29 -0.9451924827822751 1104 30-31 -1.018917542548067 1104 32-33 -0.8314136509644143 1104 34-35 -0.9194068980692762 1104 36-37 -1.1185337254361585 1104 38-39 -1.279266913311723 1104 40-41 -1.3113775548998703 1104 42-43 -1.1594040431745825 1104 44-45 -1.1693269312982935 1104 46-47 -1.1279589173463265 1104 48-49 -1.0849384863075002 1104 50-51 -1.0881719572039756 1104 52-53 -1.0872739294261677 1104 54-55 -1.1121135754621676 1104 56-57 -1.1524728066824181 1104 58-59 -1.11764221352405 1104 60-61 -1.4451675602577865 1104 62-63 -1.4183599510758853 1104 64-65 -1.5346228298622364 1104 66-67 -1.358418474816773 1104 68-69 -1.1370480951402584 1104 70-71 -1.092118391389647 1104 72-73 -1.187333325211931 1104 74-75 -1.0620387789032897 1104 76-77 -0.9747999946827335 1104 78-79 -1.1952873262610737 1104 80-81 -1.35762646272698 1104 82-83 -1.2886396665710542 1104 84-85 -1.2078737545537308 1104 86-87 -1.229879278759725 1104 88-89 -1.2919741048367648 1104 90-91 -1.1676018105907922 1104 92-93 -1.280907968434878 1104 94-95 -1.5091706276901107 1104 96-97 -1.7469374564087126 1104 98-99 -1.406273391670915 1104 100 -1.7600611669733404 1105 1 -0.3562869277910252 1105 2 -0.23295828434793364 1105 3 -0.26130235226940357 1105 4 -0.12032657917037426 1105 5 -0.23755658210026098 1105 6 -0.2667948571714476 1105 7 -0.1980413000724468 1105 8 -0.42031334513436036 1105 9 -0.5140129661683233 1105 10-11 -0.41608819707646916 1105 12-13 -0.5397790209785924 1105 14-15 -0.6113102000150974 1105 16-17 -0.4892296732372543 1105 18-19 -0.4220558964848138 1105 20-21 -0.5016178867060503 1105 22-23 -0.5754900614752998 1105 24-25 -0.5175376877227222 1105 26-27 -0.5863450495827607 1105 28-29 -0.5380422100638427 1105 30-31 -0.5591756605870017 1105 32-33 -0.40126971710687087 1105 34-35 -0.41641075909736003 1105 36-37 -0.6242260758330858 1105 38-39 -0.36550372826152255 1105 40-41 -0.43115920764572024 1105 42-43 -0.3719240775505952 1105 44-45 -0.48343889483964375 1105 46-47 -0.7034563658058275 1105 48-49 -0.6760196212176552 1105 50-51 -0.5592440433196444 1105 52-53 -0.6197288344373462 1105 54-55 -0.6669222961790453 1105 56-57 -0.699971136497652 1105 58-59 -0.5337499302481632 1105 60-61 -0.8665242050839765 1105 62-63 -0.5968913332988492 1105 64-65 -0.856953984375501 1105 66-67 -0.8660558455538805 1105 68-69 -0.9073157481307561 1105 70-71 -0.9362431047922186 1105 72-73 -0.6045807666143261 1105 74-75 -0.7721405739206517 1105 76-77 -0.7077982194253032 1105 78-79 -0.7830704343416315 1105 80-81 -0.6755214339809861 1105 82-83 -0.8245392296569918 1105 84-85 -0.7297045593475531 1105 86-87 -0.6512814027956431 1105 88-89 -0.9211609365668281 1105 90-91 -0.8838613337804837 1105 92-93 -0.7807487912008249 1105 94-95 -0.876785352917274 1105 96-97 -0.9255499954973097 1105 98-99 -0.9173511077990959 1105 100 -0.7319534471127263 1106 1 -0.3548801040554146 1106 2 -0.27015584926941827 1106 3 -0.3463055509661146 1106 4 -0.19629807497740615 1106 5 -0.1950986869707947 1106 6 -0.29718435971948054 1106 7 -0.3203709157842667 1106 8 -0.27856549285763066 1106 9 -0.5266171121916514 1106 10-11 -0.5203412203911668 1106 12-13 -0.6549407680321409 1106 14-15 -0.7817777522698961 1106 16-17 -0.7531304405477144 1106 18-19 -0.843921795363805 1106 20-21 -0.7459335125467064 1106 22-23 -0.7808889641979988 1106 24-25 -0.7666193339852896 1106 26-27 -0.8224989125459246 1106 28-29 -0.8856037783821975 1106 30-31 -0.8499582901191474 1106 32-33 -0.9145071975435783 1106 34-35 -0.8899350447029235 1106 36-37 -0.8816054955164176 1106 38-39 -0.9770146659958172 1106 40-41 -0.9621892030567736 1106 42-43 -0.7645085265345841 1106 44-45 -0.975715304432569 1106 46-47 -1.1238521451839247 1106 48-49 -0.9378207022189073 1106 50-51 -0.9692340742366099 1106 52-53 -1.139836542731274 1106 54-55 -1.096004289061284 1106 56-57 -1.2296259560557417 1106 58-59 -1.369791292523736 1106 60-61 -1.1022233181143903 1106 62-63 -1.2254160220381607 1106 64-65 -1.1539806658011358 1106 66-67 -1.0578996587319125 1106 68-69 -1.0146578308477672 1106 70-71 -1.0984000737689996 1106 72-73 -1.0272823202993067 1106 74-75 -1.165706107266594 1106 76-77 -1.1312804208821632 1106 78-79 -0.8195178690923655 1106 80-81 -0.8801434565368815 1106 82-83 -0.6528950543367635 1106 84-85 -0.7572137278145057 1106 86-87 -0.881839241938497 1106 88-89 -0.9304850056104499 1106 90-91 -1.000881263657817 1106 92-93 -1.237598410258279 1106 94-95 -1.0166053216762947 1106 96-97 -1.1508531558397976 1106 98-99 -0.9921821495494392 1106 100 -1.0617243842592785 1107 1 -0.2096482741944925 1107 2 -0.2863834224028352 1107 3 -0.38319324930434817 1107 4 -0.17939306013574452 1107 5 -0.1481439241197151 1107 6 -0.33489503522564235 1107 7 -0.3190204649198449 1107 8 -0.3713306813666861 1107 9 -0.5324326205093399 1107 10-11 -0.560971470195021 1107 12-13 -0.4445807118273777 1107 14-15 -0.5344912128962775 1107 16-17 -0.38611402089731683 1107 18-19 -0.4816648285000795 1107 20-21 -0.5552831685067829 1107 22-23 -0.5079745168855254 1107 24-25 -0.5487967069889805 1107 26-27 -0.44071655380766117 1107 28-29 -0.5870137731961123 1107 30-31 -0.5910174632117844 1107 32-33 -0.5250110900630531 1107 34-35 -0.45810178549170644 1107 36-37 -0.5686233631338098 1107 38-39 -0.43264962169245536 1107 40-41 -0.3638186830740935 1107 42-43 -0.29396283504907217 1107 44-45 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2311 26-27 0.8457392769411811 2311 28-29 0.8135113751995604 2311 30-31 0.9331996676918948 2311 32-33 1.114758708565347 2311 34-35 1.0808129482697098 2311 36-37 1.156082636274057 2311 38-39 1.2448081892832406 2311 40-41 1.186814819646294 2311 42-43 1.205206610244737 2311 44-45 1.0261910318189464 2311 46-47 1.0733483326973854 2311 48-49 1.0085489194542703 2311 50-51 1.0024433039889047 2311 52-53 1.0645306104650203 2311 54-55 0.9840466989472034 2311 56-57 1.1018174110554497 2311 58-59 0.9638320683112482 2311 60-61 1.0371251670710393 2311 62-63 1.0272865771518696 2311 64-65 1.1390017805854669 2311 66-67 1.1873391336664696 2311 68-69 1.2445177250537114 2311 70-71 1.1838967587229732 2311 72-73 1.1139564399767536 2311 74-75 0.9612610760752673 2311 76-77 0.9453109312946246 2311 78-79 0.8064791583573978 2311 80-81 0.7843839870452065 2311 82-83 0.8074588965852705 2311 84-85 0.9568438650375342 2311 86-87 0.9278508270750727 2311 88-89 1.044681350952267 2311 90-91 1.1623399751271712 2311 92-93 1.132838984572004 2311 94-95 1.252757643263429 2311 96-97 1.182904562783488 2311 98-99 1.2498895054667116 2311 100 1.63017251297153 2312 1 0.3198485675614222 2312 2 0.21507721325721008 2312 3 0.35663811204151585 2312 4 0.33087973909161406 2312 5 0.3970934008218734 2312 6 0.392353490111347 2312 7 0.4113809323241142 2312 8 0.3428735975138508 2312 9 0.488442471563836 2312 10-11 0.21172150741254114 2312 12-13 0.5447716302634049 2312 14-15 0.9806490785623154 2312 16-17 0.9436317914894872 2312 18-19 1.011181220381964 2312 20-21 1.0361981679479797 2312 22-23 0.5815940255469201 2312 24-25 0.6815673834594094 2312 26-27 0.8541694903468695 2312 28-29 1.043309309622579 2312 30-31 1.0535872578587373 2312 32-33 0.9356129302604899 2312 34-35 1.0076654988959035 2312 36-37 1.1696288761692841 2312 38-39 1.0977398970677896 2312 40-41 1.2434681996178227 2312 42-43 1.096764068463024 2312 44-45 1.0347330491206037 2312 46-47 1.2468911885282594 2312 48-49 1.315418919474233 2312 50-51 1.309604044050836 2312 52-53 1.3417863039786013 2312 54-55 1.1964608744833143 2312 56-57 1.2366112326922973 2312 58-59 1.2928896513106665 2312 60-61 1.3068964881738196 2312 62-63 1.3851848236275544 2312 64-65 1.3871922045817868 2312 66-67 1.4208842455402433 2312 68-69 1.3508285059440368 2312 70-71 1.190285303215937 2312 72-73 1.2456399670702254 2312 74-75 1.2910785761800767 2312 76-77 1.2312718558329827 2312 78-79 1.1627619882737221 2312 80-81 1.2938349101261188 2312 82-83 1.2528898606742622 2312 84-85 1.1108043184023302 2312 86-87 1.3357683397578306 2312 88-89 0.7448587398122051 2312 90-91 1.2398183280960637 2312 92-93 1.4906805804128993 2312 94-95 1.4803873807039594 2312 96-97 1.5822376325790692 2312 98-99 1.7128756777205787 2312 100 1.897217615861063 2313 1 -0.4539304409924938 2313 2 -0.0010073657138818248 2313 3 0.19621504064478046 2313 4 0.1345903427990791 2313 5 0.29190421476059214 2313 6 0.47397882731747387 2313 7 0.14737471022437632 2313 8 0.010409566575965812 2313 9 0.26133436012901257 2313 10-11 0.3933454715197371 2313 12-13 0.5504045234367965 2313 14-15 0.5588625101179971 2313 16-17 0.6993047742477856 2313 18-19 0.43540945296334854 2313 20-21 0.706407021199503 2313 22-23 0.7942328617867318 2313 24-25 0.7161444665363064 2313 26-27 0.8621006078581459 2313 28-29 0.7463175732262002 2313 30-31 0.7852192398829061 2313 32-33 0.9657482834877413 2313 34-35 0.9993928230252322 2313 36-37 0.8775741660231162 2313 38-39 0.6831152655364363 2313 40-41 0.8868364014908323 2313 42-43 0.7957932875179026 2313 44-45 0.9433187425085947 2313 46-47 0.9749408535166353 2313 48-49 0.969485033207043 2313 50-51 0.8915101309493636 2313 52-53 0.7353431843566725 2313 54-55 0.7892122361806244 2313 56-57 0.8590288872137393 2313 58-59 0.7623725275409967 2313 60-61 0.8712016535268106 2313 62-63 1.0540769730815214 2313 64-65 1.1699353423972738 2313 66-67 1.1467276347799498 2313 68-69 1.0056348671190705 2313 70-71 1.0837632304054026 2313 72-73 1.1276726242667579 2313 74-75 0.8429277726474922 2313 76-77 1.0369729625997586 2313 78-79 0.7537937887545993 2313 80-81 0.7939273491078076 2313 82-83 0.8769282623282528 2313 84-85 1.0286496025185698 2313 86-87 1.2120403565410207 2313 88-89 1.1779709224853647 2313 90-91 1.1396284481294288 2313 92-93 0.9698335058737335 2313 94-95 1.021376345402075 2313 96-97 1.2468351699261326 2313 98-99 1.3675008388758059 2313 100 1.1942895314272448 2314 1 -0.4778143521134339 2314 2 -0.3204062327022825 2314 3 -0.02518318566389155 2314 4 -0.24010099639316707 2314 5 0.1454145104457325 2314 6 0.1462182317904066 2314 7 0.12770908402667658 2314 8 0.15290932346088937 2314 9 0.30007832277127733 2314 10-11 0.44696211813337783 2314 12-13 0.5425664457048001 2314 14-15 0.4390778052024942 2314 16-17 0.578381242043136 2314 18-19 0.6048828275722187 2314 20-21 0.292794388368101 2314 22-23 0.561770039325566 2314 24-25 0.7380512800508328 2314 26-27 0.6333507723183445 2314 28-29 0.44557468982418413 2314 30-31 0.46710435418513185 2314 32-33 0.4414372525463435 2314 34-35 0.5093045293498406 2314 36-37 0.4062796211440727 2314 38-39 0.4683316760901093 2314 40-41 0.4954435332477587 2314 42-43 0.25341356274324056 2314 44-45 0.47840716012499485 2314 46-47 0.6601070679574903 2314 48-49 0.4920468273391023 2314 50-51 0.5736693455827933 2314 52-53 0.61889940495643 2314 54-55 0.6771180715144922 2314 56-57 0.44653220025252693 2314 58-59 0.4669469522163183 2314 60-61 0.5891087950083431 2314 62-63 0.47620054037101056 2314 64-65 0.5423821573990892 2314 66-67 0.6620076556783161 2314 68-69 0.6708046281798588 2314 70-71 0.764187985812562 2314 72-73 0.6892405639665995 2314 74-75 0.6898498881109489 2314 76-77 0.5017834853829761 2314 78-79 0.6671768923521313 2314 80-81 0.7502095380206164 2314 82-83 0.6766929650700213 2314 84-85 0.5451154052845553 2314 86-87 0.7785139771251472 2314 88-89 0.8240854668686453 2314 90-91 1.0342580837619018 2314 92-93 0.8474894189090882 2314 94-95 0.9659127392523104 2314 96-97 0.9773688027518155 2314 98-99 1.0413271650923797 2314 100 0.886027718418017 2315 1 -0.2216070732736135 2315 2 -0.012228966208631675 2315 3 0.08177764134937604 2315 4 -0.11802601232080434 2315 5 0.22620992040689458 2315 6 0.2810281260021057 2315 7 0.37074501749415134 2315 8 0.3316695954365301 2315 9 0.5354167985414762 2315 10-11 0.5477638091624328 2315 12-13 0.5460259823095654 2315 14-15 0.5900376027685397 2315 16-17 0.7068524769118838 2315 18-19 0.7375109955520927 2315 20-21 0.8512164185388187 2315 22-23 0.8406422546888095 2315 24-25 0.7987162273726511 2315 26-27 0.7924192665538001 2315 28-29 0.8202565870306344 2315 30-31 0.7838326017132005 2315 32-33 0.8864113183949698 2315 34-35 0.9432718785952119 2315 36-37 0.9370932983943661 2315 38-39 0.8596692016013137 2315 40-41 0.7682254524109524 2315 42-43 0.8289006604579399 2315 44-45 0.6779296009947728 2315 46-47 0.6860650657906078 2315 48-49 0.7315224391583044 2315 50-51 0.6465625782270692 2315 52-53 0.8070016405846658 2315 54-55 0.740756627552777 2315 56-57 0.7641310685804612 2315 58-59 0.7968591432471754 2315 60-61 0.8516821791181428 2315 62-63 0.7970668238145464 2315 64-65 0.924667476257774 2315 66-67 0.946487838819138 2315 68-69 0.884676339617883 2315 70-71 0.5062880581618288 2315 72-73 0.8371016713782566 2315 74-75 1.0767614204395457 2315 76-77 0.9119781624928969 2315 78-79 0.7644286101972071 2315 80-81 0.8687993392941102 2315 82-83 0.8621455439073209 2315 84-85 0.7854341919766057 2315 86-87 0.7376064977950776 2315 88-89 0.9241209216071589 2315 90-91 0.9064608488715571 2315 92-93 1.0594484641867687 2315 94-95 1.222749024747543 2315 96-97 1.2268081859473519 2315 98-99 1.2027911038438717 2315 100 1.059328500581941 2316 1 0.44978335552374915 2316 2 0.40768855628384415 2316 3 0.44911203719770043 2316 4 0.3647003964805293 2316 5 0.37972439418658155 2316 6 0.3764630125714916 2316 7 0.37026548693577155 2316 8 0.46608590139888406 2316 9 0.49732755952194196 2316 10-11 0.4391914463837949 2316 12-13 0.6387987530170278 2316 14-15 0.8355224969002535 2316 16-17 0.8628780384697095 2316 18-19 0.910473121817688 2316 20-21 0.9442689518327612 2316 22-23 0.9519829556864181 2316 24-25 0.9299111108646514 2316 26-27 1.0513963671118418 2316 28-29 0.9709399945739108 2316 30-31 0.8243273618473808 2316 32-33 0.8976669540811315 2316 34-35 0.7852899460127531 2316 36-37 0.8158634270939018 2316 38-39 0.8631660153677387 2316 40-41 0.8541053538296168 2316 42-43 0.8643588197774861 2316 44-45 0.6720567723240052 2316 46-47 0.8250412833479857 2316 48-49 0.6219973536340078 2316 50-51 0.7618472760004167 2316 52-53 0.8311952030727028 2316 54-55 0.8193935193204922 2316 56-57 0.8919803849543584 2316 58-59 0.8772200719540422 2316 60-61 0.8786816729227098 2316 62-63 0.9295720016080367 2316 64-65 1.0289378335757888 2316 66-67 1.011333309331718 2316 68-69 0.9584844854301409 2316 70-71 0.9297017387271609 2316 72-73 0.988416948950821 2316 74-75 1.1427563217664058 2316 76-77 0.9477305096572799 2316 78-79 0.9870813802402267 2316 80-81 0.9924106896119476 2316 82-83 0.9537745664780424 2316 84-85 1.0487515467863844 2316 86-87 1.0145200957430198 2316 88-89 1.0701578660052178 2316 90-91 1.1549763586470725 2316 92-93 1.2079540948459488 2316 94-95 1.0396697653840192 2316 96-97 1.4282435865804892 2316 98-99 1.3990390941431023 2316 100 1.1590721681120861 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 12.0 13 53.0 14 201.0 15 540.0 16 1441.0 17 2404.0 18 3860.0 19 5346.0 20 7204.0 21 9219.0 22 10495.0 23 10779.0 24 10969.0 25 12028.0 26 14111.0 27 16954.0 28 20516.0 29 24626.0 30 29993.0 31 36593.0 32 46128.0 33 59161.0 34 80997.0 35 111873.0 36 167270.0 37 260937.0 38 248069.0 39 39120.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.07332387760659 20.610747828772773 13.375166118695109 23.940762174925524 2 15.62587587851885 21.581159258985448 39.796913982941035 22.99605087955467 3 18.326257244663687 26.782548789265793 31.21578739562566 23.67540657044486 4 12.515235529091328 17.96906740685607 39.08729836285099 30.428398701201615 5 12.44510920475475 38.189450730509655 35.56533558434679 13.800104480388804 6 28.84498991394151 38.496734521779466 18.642188240053876 14.016087324225143 7 25.48413644292036 33.55455303951651 23.236355748584575 17.724954768978545 8 24.913457984166122 35.55302805659251 21.208785405859917 18.324728553381455 9 26.123137717506633 15.859684356420841 21.22437406672614 36.792803859346385 10-11 24.033590165212985 26.461293236796614 29.356379417919953 20.14873718007045 12-13 24.660219099222644 24.74483174351198 28.782313154524296 21.812636002741076 14-15 22.26537017012658 26.20961616109097 26.562486036719264 24.962527632063185 16-17 21.32041925267151 29.00009708307996 27.46833730805274 22.21114635619579 18-19 20.873266623717306 28.320458003732206 29.68233993307374 21.123935439476742 20-21 21.75307385248189 27.65994005231104 29.316442800488907 21.270543294718163 22-23 21.829309606655904 27.500859122581968 29.031315932072854 21.638515338689277 24-25 21.446876753027137 27.48876539363469 29.48286486422811 21.581492989110057 26-27 21.4944027023986 27.646819640646953 29.210626477315703 21.64815117963875 28-29 21.1484498258989 28.132073111088364 29.263649022580335 21.4558280404324 30-31 21.778006337662294 27.531581681791835 29.3741020257203 21.31630995482557 32-33 21.608609367033583 27.76971811960569 28.924011935787252 21.697660577573476 34-35 21.704319915490956 27.451666311159865 29.396268194330172 21.447745579019003 36-37 21.41270127636889 27.08082423556832 29.605647034348024 21.900827453714765 38-39 21.270750442951037 26.82534220278199 29.99882583949429 21.905081514772686 40-41 20.997022110958152 26.339308744659927 30.60795487549613 22.055714268885794 42-43 20.73844698977683 26.674766559385745 30.1131994535619 22.473586997275525 44-45 20.172442370253854 27.163045138561635 30.3704212260763 22.294091265108207 46-47 20.204114714078113 26.771317336587774 30.002368513958594 23.022199435375523 48-49 20.206479310024044 26.75578300904555 29.93868749063129 23.09905019029911 50-51 19.91605279119188 26.789738323095193 29.798009138155802 23.496199747557124 52-53 19.55692152684533 27.066707564551283 29.694951887430605 23.681419021172783 54-55 19.43693338684962 26.786268522836775 29.701822749512907 24.074975340800698 56-57 19.99014515384081 26.50328041261989 28.9269388678626 24.5796355656767 58-59 19.610299658560265 26.834035102685817 28.822875852837647 24.73278938591627 60-61 20.04368369656526 27.02105136591778 28.130813863744564 24.804451073772395 62-63 20.026582359208753 26.772302839039835 27.86569734212967 25.335417459621745 64-65 19.692450704477135 26.700006378667783 27.652987471402646 25.95455544545244 66-67 20.216631439234 26.667539055308765 27.20877222000287 25.90705728545437 68-69 20.20828913367035 26.76410507773857 26.949113690068305 26.078492098522776 70-71 20.18875983657558 26.470919337540188 26.817300099529096 26.523020726355135 72-73 20.131293251531748 26.382393017416227 26.77491546279977 26.71139826825225 74-75 20.07860786151754 26.41656478037867 26.737887518350707 26.766939839753086 76-77 19.967282470279137 26.250413438729396 26.795258639955204 26.987045451036256 78-79 20.037172455819622 26.053382084095063 26.823684745074143 27.08576071501117 80-81 19.789037283051734 26.045734721715586 27.055296165916932 27.109931829315748 82-83 19.970761136853806 25.49693065074755 27.35133931546279 27.180968896935852 84-85 20.19803412682995 25.380336228849547 26.989830877685232 27.43179876663526 86-87 19.84650860727125 25.459584149193244 27.329335924577563 27.364571318957942 88-89 20.135226861655646 24.849957932130504 27.41239925050095 27.6024159557129 90-91 20.20633780267185 24.26023480687679 27.560835071168622 27.97259231928274 92-93 20.165638553159468 24.104704238926484 27.756708064792683 27.97294914312136 94-95 19.663522259131085 23.78308747210677 28.460415685774283 28.09297458298786 96-97 19.809093837552652 23.688312258071853 28.459219582302385 28.043374322073113 98-99 19.752746839757965 24.02803740975743 28.183146757199395 28.036068993285205 100 19.672228388671574 24.07810679351315 28.304165034769564 27.94549978304571 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 492.0 1 335.0 2 296.5 3 427.0 4 330.5 5 254.5 6 363.0 7 557.5 8 803.5 9 982.0 10 889.0 11 720.0 12 739.0 13 836.5 14 946.0 15 1040.0 16 1170.0 17 1320.5 18 1451.0 19 1581.0 20 1740.5 21 1977.0 22 2289.5 23 2726.5 24 3256.0 25 3911.5 26 4890.5 27 6184.5 28 7474.0 29 9289.5 30 11689.0 31 13987.5 32 16925.0 33 19987.0 34 22694.5 35 25231.0 36 28014.0 37 31639.5 38 34188.0 39 35346.5 40 36323.0 41 37670.0 42 39447.0 43 42193.5 44 45855.5 45 49742.5 46 52999.0 47 54925.0 48 56519.5 49 57508.0 50 57409.5 51 56522.0 52 54352.0 53 50489.5 54 45468.5 55 39602.0 56 34112.0 57 28428.5 58 22716.0 59 18408.5 60 14111.5 61 10296.0 62 7652.5 63 5653.0 64 4031.0 65 2708.0 66 1836.0 67 1346.5 68 1023.0 69 738.0 70 568.0 71 432.5 72 317.0 73 236.5 74 178.0 75 125.0 76 72.5 77 52.0 78 38.0 79 23.5 80 16.0 81 9.0 82 3.5 83 4.0 84 5.0 85 3.5 86 3.0 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.049719434530797205 2 0.0 3 8.124090609607388E-5 4 0.018766649308193065 5 0.004387008929187989 6 0.0 7 3.249636243842955E-4 8 1.6248181219214776E-4 9 0.0020310226524018465 10-11 8.936499670568126E-4 12-13 2.8434317133625855E-4 14-15 0.0 16-17 2.031022652401847E-4 18-19 0.0 20-21 4.062045304803694E-5 22-23 0.0 24-25 4.062045304803694E-5 26-27 4.062045304803694E-5 28-29 0.0010561317792489604 30-31 9.342704201048495E-4 32-33 0.012551719991843414 34-35 0.02189442419289191 36-37 0.03127774884698844 38-39 0.019538437916105766 40-41 1.218613591441108E-4 42-43 0.0021528840115459576 44-45 0.009667667825432791 46-47 0.014420260832053111 48-49 0.006621133846830021 50-51 0.010845660963825863 52-53 0.016207560766166737 54-55 0.01048007688639353 56-57 0.0032902566968909916 58-59 0.007271061095598612 60-61 0.0010561317792489604 62-63 0.0012592340444891451 64-65 0.019700919728297916 66-67 0.046185455115618 68-69 0.05638118883067527 70-71 0.049841295889941316 72-73 0.043179541590063264 74-75 0.029937273896403223 76-77 0.03038409887993163 78-79 0.012754822257083598 80-81 0.0021122635584979207 82-83 0.028678039851914073 84-85 0.04988191634298936 86-87 0.049556952718605066 88-89 0.06109316138424756 90-91 0.036477166837137164 92-93 0.027296944448280822 94-95 0.007920988344367202 96-97 0.0032902566968909916 98-99 0.02156946056850761 100 0.01892913112038521 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1230907.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.69994255067347 #Duplication Level Percentage of deduplicated Percentage of total 1 88.02657871173652 65.75580372699055 2 8.128546579911477 12.144039250797213 3 1.82502421922212 4.089876129884402 4 0.7150209150119751 2.1364808509569806 5 0.36747950155457587 1.3725348827338466 6 0.2198544663620578 0.9853869604052813 7 0.1485191194937548 0.7766058785702064 8 0.09810199584358659 0.5862570762897861 9 0.06895733441818651 0.4635998027537499 >10 0.3434084981053303 4.642096277524527 >50 0.03136988924420754 1.661901049386341 >100 0.024690981710359775 3.3818637476063658 >500 0.001915659693264079 1.0086141672375564 >1k 5.321276925733553E-4 0.9949401988632472 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3949 0.3208203381733957 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3554 0.28873018026544656 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 1955 0.15882597141782442 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1915 0.15557633517398148 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.124090609607386E-5 0.0 5 0.0 0.0 0.0 1.6248181219214773E-4 0.0 6 1.6248181219214773E-4 0.0 0.0 1.6248181219214773E-4 0.0 7 1.6248181219214773E-4 0.0 0.0 1.6248181219214773E-4 0.0 8 1.6248181219214773E-4 0.0 0.0 1.6248181219214773E-4 0.0 9 1.6248181219214773E-4 0.0 0.0 2.437227182882216E-4 0.0 10-11 1.6248181219214773E-4 0.0 0.0 4.4682498352840625E-4 0.0 12-13 1.6248181219214773E-4 0.0 0.0 6.09306795720554E-4 0.0 14-15 1.6248181219214773E-4 8.124090609607386E-5 0.0 6.905477018166279E-4 0.0 16-17 1.6248181219214773E-4 8.124090609607386E-5 0.0 9.748908731528865E-4 0.0 18-19 1.6248181219214773E-4 8.124090609607386E-5 0.0 0.0015029567627773666 0.0 20-21 1.6248181219214773E-4 8.124090609607386E-5 0.0 0.0028840521664106225 0.0 22-23 1.6248181219214773E-4 8.124090609607386E-5 0.0 0.010358215527249418 0.0 24-25 1.6248181219214773E-4 8.124090609607386E-5 0.0 0.02571274677940738 0.0 26-27 1.6248181219214773E-4 8.124090609607386E-5 0.0 0.053172173039880345 0.0 28-29 1.6248181219214773E-4 8.124090609607386E-5 0.0 0.14663983550341333 0.0 30-31 1.6248181219214773E-4 8.124090609607386E-5 0.0 0.4097385098955486 0.0 32-33 1.6248181219214773E-4 8.124090609607386E-5 0.0 0.9441005697424745 0.0 34-35 1.6248181219214773E-4 8.124090609607386E-5 0.0 1.7213729388166612 0.0 36-37 1.6248181219214773E-4 8.124090609607386E-5 0.0 2.808944948724802 0.0 38-39 1.6248181219214773E-4 8.124090609607386E-5 0.0 4.132440549935941 0.0 40-41 1.6248181219214773E-4 8.124090609607386E-5 0.0 5.420840079713577 0.0 42-43 1.6248181219214773E-4 8.124090609607386E-5 0.0 6.593511938757356 0.0 44-45 1.6248181219214773E-4 8.124090609607386E-5 0.0 7.940201818658924 0.0 46-47 1.6248181219214773E-4 8.124090609607386E-5 0.0 9.43430332267182 0.0 48-49 1.6248181219214773E-4 8.124090609607386E-5 0.0 10.845295379748428 0.0 50-51 1.6248181219214773E-4 8.124090609607386E-5 0.0 12.157945319995743 0.0 52-53 1.6248181219214773E-4 8.124090609607386E-5 0.0 13.339188094632657 0.0 54-55 1.6248181219214773E-4 8.124090609607386E-5 0.0 14.571937603734483 0.0 56-57 1.6248181219214773E-4 8.124090609607386E-5 0.0 15.912493795225798 0.0 58-59 1.6248181219214773E-4 8.124090609607386E-5 0.0 17.29277678979809 0.0 60-61 1.6248181219214773E-4 8.124090609607386E-5 0.0 18.60530486868626 0.0 62-63 1.6248181219214773E-4 8.124090609607386E-5 0.0 19.825421416890148 0.0 64-65 1.6248181219214773E-4 8.124090609607386E-5 0.0 20.990253528495654 0.0 66-67 1.6248181219214773E-4 8.124090609607386E-5 0.0 22.255621261395053 0.0 68-69 1.6248181219214773E-4 8.124090609607386E-5 0.0 23.553810320357265 0.0 70-71 1.6248181219214773E-4 8.124090609607386E-5 0.0 24.831161086905837 0.0 72-73 1.6248181219214773E-4 8.124090609607386E-5 0.0 26.025443026971168 0.0 74-75 1.6248181219214773E-4 8.124090609607386E-5 0.0 27.144780231162873 0.0 76-77 1.6248181219214773E-4 8.124090609607386E-5 0.0 28.308718692801325 0.0 78-79 1.6248181219214773E-4 8.124090609607386E-5 0.0 29.499060448921 0.0 80-81 1.6248181219214773E-4 8.124090609607386E-5 0.0 30.671163621622107 0.0 82-83 1.6248181219214773E-4 8.124090609607386E-5 0.0 31.800859041341063 0.0 84-85 1.6248181219214773E-4 8.124090609607386E-5 0.0 32.85609717062296 0.0 86-87 1.6248181219214773E-4 8.124090609607386E-5 0.0 33.91214770896583 0.0 88 1.6248181219214773E-4 8.124090609607386E-5 0.0 34.69961581175507 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2160 0.0 53.10924 1 GTATCAA 3550 0.0 36.68474 1 CTGCGCG 55 0.0027221134 34.168922 9 ATACCGA 235 0.0 29.988682 6 CAACGCA 4335 0.0 29.913803 5 ATCAACG 4350 0.0 29.814283 3 GTGGTAT 795 0.0 29.569017 1 TATCAAC 4445 0.0 29.388514 2 AACGCAG 4665 0.0 28.19943 6 TCAACGC 4615 0.0 28.102304 4 ATGGGCG 120 5.8589394E-6 27.407436 5 TATACCG 240 0.0 27.407436 5 GTACTAG 565 0.0 26.627815 1 CGTTAAC 160 6.895789E-8 26.44579 1 GTACATG 3860 0.0 25.699766 1 TACCGAC 220 8.913048E-11 25.626694 7 TACCTGG 735 0.0 25.572739 2 ACGCAGA 5190 0.0 25.075315 7 CGCAGAG 5330 0.0 24.240383 8 TACATGG 4085 0.0 24.041344 2 >>END_MODULE