FastQCFastQC Report
Mon 6 Jun 2016
ERR1211447_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211447_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733029
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138030.5050440372202417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111580.4082649690142329No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62620.2291230718737342No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43190.15802979039007636No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40830.1493946826030752No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30270.11075623420022254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55100.056.004251
TACCTGG25150.045.8413772
GTATCAA102250.041.1912961
ACCTGGG28700.039.147143
ATCAACG119500.034.8144383
CAACGCA120200.034.4533655
TATCAAC123650.033.946912
TCAACGC124500.033.452194
TAACGCA2700.033.0788234
AACGCAG127600.032.826686
ACGCAGA141350.029.3008377
CGCAGAG142650.028.965258
GTGGTAT21050.027.5286081
GTACCTG42500.027.4912171
GCAGAGT151100.026.7834859
CCTGGGG26800.026.4851464
GTACATG85850.025.7373491
TACATGG86100.024.813172
ACATGGG86000.024.4339183
ATGGGGG41900.024.1204075
Produced by FastQC (version 0.11.5)