FastQCFastQC Report
Mon 6 Jun 2016
ERR1211447_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211447_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733029
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122920.449757393719569No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105990.3878114721797683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60510.2214027000811188No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44370.16234734428357694No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32720.11972064694520256No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28620.1047189766372768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55750.055.4150351
TACCTGG24450.043.6314542
GTATCAA98600.039.5829621
ACCTGGG26500.039.0155263
ATCAACG117400.032.7450643
CAACGCA119200.032.244695
TATCAAC121000.032.042122
TCAACGC122900.031.3196224
AACGCAG127450.030.6724726
ACGCAGA141050.027.2486847
GTACATG88900.027.1345561
GTGGTAT22950.026.8408151
CGCAGAG143350.026.7131568
TACATGG88400.026.5277862
CCTGGGG25150.025.7884714
ACATGGG87850.025.7314323
GTACCTG44550.025.1209681
GCAGAGT153050.024.8052339
CATGGGG58050.024.7744314
TGGTATC24600.023.8796312
Produced by FastQC (version 0.11.5)